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    Wyświetlanie 1-26 z 26
    Tytuł:
    Regulation of β1 integrin expression in endothelial cells by chimeric tRNAVal ribozyme
    Autorzy:
    Papiewska-Pająk, Izabela
    Antoszczyk, Sławomir
    Powiązania:
    https://bibliotekanauki.pl/articles/1041244.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    integrins
    cell migration
    β1 integrin subunit
    hammerhead ribozyme
    Opis:
    To downregulate expression of the β1 integrin subunit in endothelial cells, plasmid encoding the ribozyme cassette containing hammerhead ribozyme flanked at the 5' terminus by tRNAVal and at the 3' terminus by constitutive transport element sequences was constructed. When used to transfect immortalized human endothelial cell line EA.hy 926, it selectively blocked the synthesis of the β1 integrin subunit and thus inhibited migration and proliferation of the cells. Thus, this construct may be a valuable tool to control the proangiogenic phenotype of stimulated endothelial cells.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 2; 311-316
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Partial deglycosylation of α subunit modifies sturgeon gonadotropin function.
    Autorzy:
    Zenkeviès, Henriks
    Vose, Vija
    Vosekalne, Ilze
    Bcena, Ausma
    Powiązania:
    https://bibliotekanauki.pl/articles/1044331.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    α subunit
    CD spectrum
    sturgeon GTH
    gonadotropic hormone
    deglycosylation
    Opis:
    Chemical deglycosylation (dg) of sturgeon Acipenser gueldenstaedti Br. (αGTH) resulted in the loss of 83% of its initial carbohydrate content. It altered also recombinant dgαGTH + βGTH dimer molecule, reducing its immunoreactivity by 30%, and fully blocking the hormonal function. CD spectroscopy showed that deglycosylation led to changes in the secondary structure of dgαGTH and in the α-β recombinant. The sugar moiety of sturgeon αGTH is suggested to play an important role in maintaining the biological function of the hormone dimer molecule.
    Źródło:
    Acta Biochimica Polonica; 2000, 47, 3; 815-819
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Additional copies of the NOG2 and IST2 genes suppress the deficiency of cohesin Irr1p/Scc3p in Saccharomyces cerevisiae.
    Autorzy:
    Białkowska, Agnieszka
    Kurlandzka, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1043770.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    60S ribosomal subunit
    sister chromatid cohesion
    salt tolerance
    Opis:
    The protein encoded by the IRR1/SCC3 gene is an element of the cohesin complex of Saccharomyces cerevisiae, responsible for establishing and maintaining sister chromatid cohesion during mitotic cell division. We noticed previously that lowering the level of expression of IRR1/SCC3 affects colony formation on solid support. Here we describe two dosage suppressors (IST2, NOG2) overcoming the inability to form colonies of an Irr1p-deficient strain. Ist2 is probably involved in osmotolerance, Nog2p is a putative GTPase required for 60S ribosomal subunit maturation, but may also participate in mRNA splicing.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 2; 421-425
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ultrastructure and SSU rDNA Phylogeny of Paraphysomonas vestita (Stokes) De Saedeleer Isolated from Laguna de Bay, Philippines
    Autorzy:
    Petronio, Joy Ann G.
    Rivera, Windell L.
    Powiązania:
    https://bibliotekanauki.pl/articles/763493.pdf
    Data publikacji:
    2010
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Paraphysomonas vestita, chrysophyte, protist, ultrastructure, small subunit ribosomal RNA gene, Philippines
    Opis:
    Paraphysomonas vestita is a unicellular, colorless, silica-scaled chrysophyte that plays an important ecological role in freshwater microbial communities as a consumer of prokaryotic and eukaryotic prey. There is little biogeographical information for this minute protist despite its significant role in aquatic food webs. In addition, the phylogenetic relationship of P. vestita to other taxa is unclear as P. vestita may be polyphyletic or a cryptic species complex. In this study, a clonal isolate from a freshwater sample of Laguna de Bay, Philippines was subjected to morphological study by electron microscopy and its small subunit ribosomal RNA (SSU rRNA) gene was sequenced. Morphological studies showed that the isolate possesses two unequal flagella emerging from the anterior part of the cell. Negative staining revealed the structure of the scales which consist of a baseplate with slightly thickened rim. The narrowing spine arises from the center of the baseplate. These results agree with previously studied isolates of P. vestita. The 18S rRNA gene sequence of the isolate had a very high similarity (99%) to P. vestita strain PV10. Phylogenetic analysis also showed that the isolate clustered with other Paraphysomonas sequences with high bootstrap support. Phylogenetic studies confirmed that P. vestita may be polyphyletic. No studies on the ultrastructure and phylogeny of a silica-scaled chrysophyte isolated in the Philippines have been reported so far. Results from this study may contribute to further ultrastructural and phylogenetic studies on aquatic flagellates and specifically to a revision of this potentially polyphyletic species.
    Źródło:
    Acta Protozoologica; 2010, 49, 2
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Production and characterization of swine hyperimmune serum against recombinant, common antigens of Gram-negative outer membrane bacteria
    Autorzy:
    Rząsa, A.
    Pietrasina, O.
    Czerniecki, M.
    Bajzert, J.
    Stefaniak, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087590.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    swine
    recombinant outer membrane proteins
    antibody response
    hyperimmune serum
    subunit vaccine
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 1; 117-125
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Isolation and expression pattern of RGS21 gene, a novel RGS member
    Autorzy:
    Li, Xin
    Chen, Lei
    Ji, Chaoneng
    Liu, Bing
    Gu, Jiefeng
    Xu, Jian
    Zou, Xianqiong
    Gu, Shaohua
    Mao, Yumin
    Powiązania:
    https://bibliotekanauki.pl/articles/1041351.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    RGS21
    G-protein signaling pathway
    G-protein α subunit (Gα)
    Opis:
    Regulators of G-protein signaling (RGS) proteins are known for the RGS domain that is composed of a conserved stretch of 120 amino acids, which binds directly to activated G-protein α subunits and acts as a GTPase-activating protein (GAP), leading to their deactivation and termination of downstream signals. In this study, a novel human RGS cDNA (RGS21), 1795 bp long and encoding a 152-amino acid polypeptide, was isolated by large-scale sequencing analysis of a human fetal brain cDNA library. Unlike other RGS family members, RGS21 gene has no additional domain/motif and may represent the smallest known member of RGS family. It may belong to the B/R4 subfamily, which suggests that it may serve exclusively as a negative regulator of αi/o family members and/or αq/11. PCR analysis showed that RGS21 mRNA was expressed ubiquitously in the 16 tissues examined, implying general physiological roles.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 4; 943-946
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Policemen of Anti-Terrorist Subunits in Scientific Research
    Autorzy:
    Zubrzycki, Waldemar
    Powiązania:
    https://bibliotekanauki.pl/articles/1861556.pdf
    Data publikacji:
    2016
    Wydawca:
    Wyższa Szkoła Bezpieczeństwa Publicznego i Indywidualnego Apeiron w Krakowie
    Tematy:
    The Police
    anti-terrorism
    fighting terrorism
    anti-terrorist subunit
    police special commando
    Opis:
    Service in a special unit of the Police is extremely tedious, both in physical and mental terms, with very high expectations for their policemen. It cannot arise only from official duties, but – above all – specific lifestyle, which should be the primary stimulus for taking them. It is confirmed by research conducted among policemen-antiterrorists, relating to their motivation in taking service in anti-terrorist subunit of the Police. Studies have also shown that such service is not fully in line with the expectations of the officers, and their priorities change over its course. A lot of factors have impact on it, negatively affecting the attitude of policemen, which were revealed in the study. The results can be used to create such conditions of service, which will reduce or eliminate loss of idealistic motives for taking service in the special unit of the Police.
    Źródło:
    Security Dimensions; 2016, 17(17); 30-41
    2353-7000
    Pojawia się w:
    Security Dimensions
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Calcareous nannofossils and foraminifera from the youngest deposits of the Siary Subunit (Oligocene, Magura Nappe, Polish Outer Carpathians)
    Autorzy:
    Garecka, M.
    Szydło, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/191190.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Geologiczne
    Tematy:
    calcareous nannoplankton
    foraminifera
    palaeoenvironment
    Gładyszów Beds
    Siary Subunit
    Magura Nappe
    Outer Carpathians
    Oligocene
    Opis:
    The calcareous nannoplankton and foraminifera from the Gładyszów Beds, regarded as the youngest deposits in the northern marginal Siary Subunit of the Magura Nappe in Poland, are characterized. The material investigated comes from outcrops in the vicinity of Gładyszów and from the Gładyszów PIG-1 borehole. The analysis allowed the establishment of the age of these deposits as not older than late Rupelian–early Chattian (the calcareous nannoplankton NP24 Zone). The taxonomic affiliations, environmental associations and preservation of the microfossils were analyzed to document the sedimentary processes and environmental conditions during the geotectonic transformation of the Magura Basin in the Oligocene. These microfossils also were compared with those reported from the terminal deposits of the Outer Carpathians and the Podhale Basin.
    Źródło:
    Annales Societatis Geologorum Poloniae; 2015, 85, 1; 205-219
    0208-9068
    Pojawia się w:
    Annales Societatis Geologorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Geology of the Magura Nappe in the Osielec area with emphasis on an Eocene olistostrome with metabasite olistoliths (Outer Carpathians, Poland)
    Autorzy:
    Cieszkowski, M.
    Kysiak, T.
    Szczęch, M.
    Wolska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/191808.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Geologiczne
    Tematy:
    Magura Nappe
    Rača Subunit
    Late Cretaceous-Palaeogene
    lithostratigraphy
    tectonics
    olisto- strome
    olistoliths
    metabasite
    Opis:
    The Magura Nappe in the Polish sector of the Outer Carpathians consists of four tectonic subunits characterized by differing development of facies. From the south to the north, they include the Siary, Rača, Bystrica and Krynica subunits. The sedimentary succession in the Rača Subunit in the vicinity of the village of Osielec is composed of Campanian–Palaeogene flysch deposited in the Magura Basin. In this succession, the Middle Eocene Pasierbiec Sandstone Fm consists of thick-bedded sandstones and conglomerates with occasional intercalations of thin-bedded shale-sandstone flysch. Within the Pasierbiec Sandstone Fm at Osielec there is an olistostrome, rich in pebbles and cobbles of exotic rocks. In addition, large blocks of Neoproterozoic metabasites and boulders of Palaeogene organogenic limestones were found. The discovery of metabasites raised the possibility that the rocks in question could be evidence of supposed oceanic crust in the basement of the Magura sedimentary basin, because of the suggestion that they represent the Alpine orogenic cycle. This concept was abandoned when investigations of the absolute age of the metabasites gave a date of ca. 600 Ma. In the Osielec area, there are two tectonic thrust sheets in the Rača Subunit, namely the Osielczyk Thrust Sheet in the north and the Bystra Thrust Sheet in the south; they are folded and cut by a transverse system of strike-slip and oblique faults. The Osielczyk Thrust Sheet was overthrust northwards on to the Siary Subunit.
    Źródło:
    Annales Societatis Geologorum Poloniae; 2017, 87, 2; 169-182
    0208-9068
    Pojawia się w:
    Annales Societatis Geologorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Structure and functions of 5S rRNA.
    Autorzy:
    Barciszewska, Mirosława
    Szymański, Maciej
    Erdmann, Volker
    Barciszewski, Jan
    Powiązania:
    https://bibliotekanauki.pl/articles/1044186.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    50S subunit structure
    ribosomal 5S RNA
    5S rRNA
    crystal structure
    Opis:
    The ribosome is a macromolecular assembly that is responsible for protein biosynthesis in all organisms. It is composed of two-subunit, ribonucleoprotein particles that translate the genetic material into an encoded polypeptides. The small subunit is the site of codon-anticodon interaction between the messenger RNA (mRNA) and transfer RNA (tRNA) substrates, and the large subunit catalyses peptide bond formation. The peptidyltransferase activity is fulfilled by 23S rRNA, which means that ribosome is a ribozyme. 5S rRNA is a conserved component of the large ribosomal subunit that is thought to enhance protein synthesis by stabilizing ribosome structure. This paper shortly summarises new results obtained on the structure and function of 5S rRNA.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 1; 191-198
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Morphology and Molecular Phylogeny of Pseudouroleptus jejuensis nov. spec., a New Soil Ciliate (Ciliophora, Spirotrichea) from South Korea
    Autorzy:
    JUNG, Jae-Ho
    Park, Kyung-Min
    Min, Gi-Sik
    Powiązania:
    https://bibliotekanauki.pl/articles/763471.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Pseudouroleptus jejuensis, soil ciliate, morphology, small subunit ribosomal RNA gene, oxytrichid, South Korea
    Opis:
    A new soil ciliate, Pseudouroleptus jejuensis, was discovered from Jeju Island, South Korea and described based on live observation, protargol impregnation, and SSU rRNA gene sequence analyses. Pseudouroleptus jejuensis differs from other congeneric species mainly by number of dorsal kineties (5 vs. 4). Based on our observation of late dividers, we confirm that the dorsal kinety anlage 3 forms 3 kineties (i.e., dorsal kineties 3–5), and the dorsal kinety anlagen 1–3 form 3–5/1–2/0 caudal cirri, respectively. Our gene trees support the assignment of this new species in Pseudouroleptus to full supporting values.
    Źródło:
    Acta Protozoologica; 2014, 53, 2
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The uselessness of cytochrome oxydase gene analysis for genetic identification of selected Gobiidae species from some European countries
    Autorzy:
    Kiełpińska, Jolanta
    Czerniejewski, Przemysław
    Adamkowska, Natalia
    Uzunova, Eliza
    Powiązania:
    https://bibliotekanauki.pl/articles/2203561.pdf
    Data publikacji:
    2022
    Wydawca:
    Instytut Technologiczno-Przyrodniczy
    Tematy:
    cytochrome oxidase subunit 1 gene
    COI gene
    Gobiidae
    invasive species
    native species
    mt DNA
    Opis:
    The family Gobiidae is the largest teleost family, consisting of more than 2,000 species in more than 200 genera. They have adapted to a wide range of habitats, inhabiting mostly marine and brackish waters. Recently invasive Ponto-Caspian gobies have caused significant changes to the structure of fish assemblage throughout the European waters. The aim of the study was to verify the possibility of identifying three species of the genus Gobiidae based on the analysis of the mitochondrial region of the cytochrome oxidase subunit 1 (COI) gene. 339 samples originated from seven countries and 17 different sites were investigated. Qualitative and quantitative assessment of the extracted DNA was conducted by measuring light absorbance and electrophoretic separation. Approximately 650-bp (base pairs) fragments were amplified from the 5' region of the mitochondrial COI with the PCR (polymerase chain reaction) reaction. Sequences of 652 bp were obtained and compared with reference sequences in the National Center for Biotechnology Information (NCBI). Based on the sequence analysis, we determined that in the analysed geographical regions, the goby preliminary identified morphologically as the round goby was represented by three species: round goby (Neogobius melanostomus), tubenose goby (Proterorhinus marmoratus), and monkey goby (Neogobius fluviatilis). The latter two are newly reported for their new habitats. Genetic characterisation of such populations offers the opportunity to assess their genetic stability, which provides agencies managing the aquatic environment in EU countries with a tool for revising the legislation, including regulations relevant for updating the status of native and invasive species in European waters.
    Źródło:
    Journal of Water and Land Development; 2022, Special Issue; 8--14
    1429-7426
    2083-4535
    Pojawia się w:
    Journal of Water and Land Development
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influenza virus hemagglutinin as a vaccine antigen produced in bacteria
    Autorzy:
    Sączyńska, Violetta
    Powiązania:
    https://bibliotekanauki.pl/articles/1039263.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    recombinant influenza hemagglutinin
    recombinant influenza vaccine
    subunit influenza vaccine
    bacterial hemagglutinin
    prokaryotic protein production
    Opis:
    Recombinant subunit vaccines based on hemagglutinin proteins produced in bacteria (bacterial HAs) are promising candidates for enhancing the supply of vaccines against influenza, especially for a pandemic. Over 20 years after the failure to obtain the antigen with native HA characteristics in the early 1980’s, there are increasing data on successful production of HA proteins in bacteria. The vast majority of bacterial HAs have been based on the HA1 subunit of HA expressed separately or as a component of conjugate vaccines, but those based on the ectodomain and the HA2 subunit have also been reported. The most of HAs have been efficiently expressed as insoluble aggregates called inclusion bodies. Refolded and purified proteins were extensively studied for structure, the ability to bind to sialic acid-containing receptors, antigenicity, immunogenicity and efficacy. The results from these studies contradict the view that glycosylation determines the correct structure of the hemagglutinin, as they proved that bacterial HAs can be valuable vaccine antigens when appropriate folding and purification methods are applied to rationally designed proteins. The best evidence for success in bacterial production of protective HA is that vaccines based on proprietary Toll-like Receptor (VaxInnate) and bacteriophage Qβ-VLPs (Cytos Biotechnology) technologies have been advanced to clinical studies.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 3; 561-572
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cyclic AMP deficiency stimulates a stress condition in tobacco BY-2 cells
    Autorzy:
    Sgobba, A.
    Sabetta, W.
    Blanco, E.
    Paradiso, A.
    Viggiano, L.
    De Pinto, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/80076.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    cyclic amphipathic peptide
    stress condition
    tobacco
    BY-2 cell
    cell cycle progression
    plant
    subunit
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Immune signalling and vesicular trafficking – intertwining networks regulated by E3 ubiquitin ligases
    Autorzy:
    Stegmann, M.
    Furlan, G.
    Anderson, R.G.
    Ichimura, K.
    McDowell, J.M.
    Shirasu, K.
    Trujillo, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/80842.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    pattern recognition receptor
    pathogen-associated molecular pattern
    ubiquitin ligase
    subunit
    exocytosis
    immune response
    vesicular trafficking
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    "Hauffenia" Pollonera, 1898 (Caenogastropoda: Hydrobiidae) in Slovakia: a preliminary report
    Autorzy:
    Steffek, J.
    Falniowski, A.
    Szarowska, M.
    Grego, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/84536.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
    Tematy:
    Hauffenia
    Caenogastropoda
    Hydrobiidae
    Slovakia
    hydrobiid
    valvatiform
    morphology
    shell
    penis
    cytochrome oxidase subunit 1 gene
    gastropod
    snail
    Opis:
    We studied the shell and penis morphology, and cytochrome oxidase subunit I (COI) gene sequences, in minute, valvatiform hydrobiid gastropods from Slovensky Kras, Slovakia. The morphology confirmed the assignment of the studied snails to the genus Hauffenia, while in the molecular tree they were placed within the Hydrobiidae, and not close to Hauffenia. The results indicate that the Slovak valvatiform hydrobiids are two taxa which presumably represent two genera: Hauffenia Pollonera, 1898 and Lobaumia Haase, 1993. More molecular data on the Austrian and Hungarian taxa are needed.
    Źródło:
    Folia Malacologica; 2011, 19, 1
    1506-7629
    Pojawia się w:
    Folia Malacologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Functional conservation between yeast and plant mitochondrial m-AAA proteases
    Autorzy:
    Skibior, R.
    Kolodziejczak, M.
    Janska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/80313.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    FtsH protease
    bifunctional enzyme
    ATPase
    plant mitochondrion
    yeast
    AAA protease
    Arabidopsis
    ribosomal subunit
    immunoblotting
    biogenesis
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    High light induced accumulation of two isoforms of the CF1 α-subunit in mesophyll and bundle sheath chloroplasts of C4 plants
    Autorzy:
    Romanowska, Elżbieta
    Powikrowska, Marta
    Zienkiewicz, Maksymilian
    Drożak, Anna
    Pokorska, Berenika
    Powiązania:
    https://bibliotekanauki.pl/articles/1040835.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    bundle sheath
    light intensity
    C_4 subtypes
    chloroplasts
    CF_1 α-subunit isoforms
    mesophyll
    ATP synthase
    Opis:
    The effect of light irradiance on the amount of ATP synthase α-subunit in mesophyll (M) and bundle sheath (BS) chloroplasts of C4 species such as maize (Zea mays L., type NADP-ME), millet (Panicum miliaceum, type NAD-ME) and guinea grass (Panicum maximum, type PEP-CK) was investigated in plants grown under high, moderate and low light intensities equal to 800, 350 and 50 µmol photons m-2 s-1, respectively. The results demonstrate that α-subunit of ATP synthase in both M and BS chloroplasts is altered by light intensity, but differently in the investigated species. Moreover, we identified two isoforms of the CF1 α-subunit, called α and ά. The CF1 α-subunit was the major isoform and was present in all light conditions, whereas ά was the minor isoform in low light. A strong increase in the level of the ά-subunit in maize mesophyll and bundle sheath thylakoids was observed after 50 h of high light treatment. The α and ά-subunits from investigated C4 species displayed apparent molecular masses of 64 and 67 kDa, respectively, on SDS/PAGE. The presence of the ά-subunit of ATPase was confirmed in isolated CF1 complex, where it was recognized by antisera to the α-subunit. The N-terminal sequence of ά-subunit is nearly identical to that of α. Our results indicate that both isoforms coexist in M and BS chloroplasts during plant growth at all irradiances. We suggest the existence in M and BS chloroplasts of C4 plants of a mechanism(s) regulating the ATPase composition in response to light irradiance. Accumulation of the ά isoform may have a protective role under high light stress against over protonation of the thylakoid lumen and photooxidative damage of PSII.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 1; 175-182
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Interplay of two PP2A B subunits in high light acclimation
    Autorzy:
    Konert, G.
    Trotta, A.
    Rahikainen, M.
    Kangasjarvi, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/80957.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    reactive oxygen species
    homeostasis
    alternative oxidase
    proteomic analysis
    signalling pathway
    PP2A phosphatase
    B subunit
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic variation in the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of Enterobius vermicularis (Nematoda) in Poland: a preliminary study
    Autorzy:
    Kubiak, K.
    Paukszto, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/6116.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    genetic variation
    mitochondrial cytochrome c
    cytochrome C oxidase subunit 1
    Enterobius vermicularis
    Nematoda
    parasite
    intestinal parasite
    human parasite
    Polska
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Phylogenetic relationships of Alzoniella slovenica (Lozek et Brtek, 1964) (Caenogastropoda: Hydrobiidae)
    Autorzy:
    Szarowska, M.
    Falniowski, A.
    Steffek, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/83587.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
    Tematy:
    cytochrome oxidase subunit 1
    mtDNA
    phylogenetic relationship
    Alzoniella slovenica
    Caenogastropoda
    Hydrobiidae
    Rissooidea
    morphology
    phylogenesis
    shell
    radula
    penis
    reproductive organ
    female
    snail
    Źródło:
    Folia Malacologica; 2011, 19, 2
    1506-7629
    Pojawia się w:
    Folia Malacologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cacao swollen shoot virus detection and DNA barcoding of its vectors and putative vectors in Theobroma cacao L. by using polymerase chain reaction
    Autorzy:
    Obok, E.E.
    Aikpokpodion, P.O.
    Ani, O.C.
    Allainguillaume, J.
    Wetten, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2096411.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Cacao swollen shoot virus
    COI – cytochrome c oxidase subunit I
    DNA barcoding
    Jack Beardsley
    mealybug
    PCR – polymerase chain reaction
    Theobroma cacao
    Opis:
    Cacao swollen shoot virus (CSSV) is an endemic pathogen causing significant economic losses to cacao (Theobroma cacao L.) production in West Africa. There is limited updated report on the occurrence, spread, genetic diversity and species of CSSV and its mealybug vectors, especially in Nigeria. Nigeria is presently lagging behind in the search for resistance to CSSV and its vectors in T. cacao L. The present study aimed to map and screen for the presence of CSSV and its natural vectors – female mealybugs (Pseudococcidae: Hemiptera) in cacao plantations in Nigeria. Symptomatic and asymptomatic cacao leaves and whole female mealybug samples were collected from major cacao-growing areas in Nigeria – Abia, Akwa Ibom, Cross River, Edo, Ondo and Oyo States. A total of 2568 cacao leaves from 1052 cacao trees were screened with polymerase chain reaction (PCR) using an open reading frame 1 (ORF 1) CSSV-specific primer pair. PCR screening of the mealybug species was performed using the cytochrome c oxidase subunit I (COI) gene. A combination of scanning electron microscopy (SEM) and histology for morphological identification and DNA barcoding enabled to characterise the female mealybug species. The results revealed that CSSV and its mealybug vectors are present in the major cacao-growing areas in Nigeria. Although CSSV and its vectors have been previously reported in Cross River, Ondo and Oyo States, our results present the first documented evidence of CSSV emergence and its mealybug vectors in Abia, Akwa Ibom and Edo States. We also present the first report of Pseudococcus jackbeardsleyi (Gimpel and Miller) mealybug species on cacao in Nigeria. In conclusion, it is pertinent to re-establish coordinated routine survey and monitoring of CSSV and its mealybug vector presence in T. cacao L. in Nigeria.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2021, 102, 3; 229-244
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Local effect of progesterone infusion into ovarian artery on activin A and inhibin alpha-subunit secretion during the middle luteal phase in gilts
    Autorzy:
    Wasowska, B
    Stefanczyk-Krzymowska, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/30684.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    dimeric glycoprotein
    activin
    inhibin
    growth factor-beta
    progesterone infusion
    ovarian artery
    alpha-subunit
    secretion
    exogenous progesterone
    local feedback
    gilt
    middle luteal phase
    Opis:
    The present study was undertaken to elucidate whether an increased, but physiological, amount of progesterone (P4) supplied to the porcine corpus luteum affects luteal secretion of activin A and inhibin a-subunit (Inha) in freely moving gilts. On day 9 of the estrous cycle (EC), both ovarian arteries and both ovarian veins of gilts (n=5) were cannulated. Progesterone was infused into the right ovarian arteries in gilts on days 10, 11 and 12 of the EC at a rate adequate to its physiological retrograde transfer found during the middle luteal phase of the EC. The P4 infusion rate was 0.62 μg/min (day 10), 2x0.62 μg/min (day 11) and 3x0.62 μg/min (day 12). The left ovarian arteries were infused with saline (control). Blood samples were collected from both ovarian veins on days 10-12 of the EC before and after P4 or saline infusion. The mean plasma activin A level in the ovarian vein ipsilateral to the P4-infused ovary was higher (PcO.OOOl) on days 10-12 of the EC than this found in the contralateral ovarian vein. The level of activin A in the ovarian vein ipsilataral to the infusion of P4 was higher on days 11 (PcO.Ol) and 12 (P<0.0001) and tended to be higher (P<0.07) on day 10 of the EC than this in contralateral ovarian vein. The level of Inha in the ovarian vein ipsilateral to the P4-infused ovary on days 10-12 of the EC was not significantly different (P>0.05) than this found in the contralateral ovarian vein. The results of the present study indicate that a local elevation of P4 concentration in blood supplying the ovary during the middle luteal phase of the porcine EC affects ovarian secretion of activin A. The effect of P4 on the secretion of activin A suggested the existence of a short regulatory loop of a positive feedback between P4 being retrogradely transferred into the ovary and the secretion of this peptide.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 1; 97-104
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic studies on the invasive slug Arion lusitanicus Mabille, 1868 (Gastropoda: Pulmonata) in Poland
    Autorzy:
    Soroka, M.
    Kaluski, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/83651.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Mikołaja Kopernika. Wydział Biologii i Ochrony Środowiska. Stowarzyszenie Malakologów Polskich
    Tematy:
    animal genetics
    invasive species
    slug
    Arion lusitanicus
    pest
    Gastropoda
    Pulmonata
    Polska
    migration route
    DNA
    mitochondrial gene
    Arionidae
    cytochrome oxidase subunit 1 gene
    Opis:
    Within the last decades the slug Arion lusitanicus has expanded its range over wide areas of Europe, in most of them it is now a serious pest. Poland has been invaded relatively recently (since the late 1980s). Considering the ecological importance of the slug, very little is known yet about the mechanism of invasion, establishment of new populations and influence on the native fauna and flora. The analysis of nucleotide sequences of mitochondrial cytochrome oxidase subunit 1 gene (cox1) revealed a great inter- and intrapopulation variation in the Polish populations of A. lusitanicus. The differentiation of all the studied Polish populations of A. lusitanicus is 0.2–2.2%, while two analysed Belgian populations are monomorphic and moderately genetically diverse at 0.8%. This indicates a heterogeneous origin of the Polish populations, probably resulting from multiple independent introduction events. The genotype found in the first four Polish populations (S. Poland) suggests that their origin is different from the remaining populations.
    Źródło:
    Folia Malacologica; 2011, 19, 4
    1506-7629
    Pojawia się w:
    Folia Malacologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Geological characterisation of the Krynica Subunit in the vicinity of Krościenko on the Dunajec river (Magura Nappe, Outer Flysch Carpathians)
    Charakterystyka geologiczna podjednostki krynickiej w okolicach Krościenka nad Dunajcem (płaszczowina magurska, zewnętrzne Karpaty fliszowe)
    Autorzy:
    Chrustek, M.
    Golonka, J.
    Janeczko, A.
    Stachyrak, F.
    Powiązania:
    https://bibliotekanauki.pl/articles/184009.pdf
    Data publikacji:
    2005
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Wydawnictwo AGH
    Tematy:
    płaszczowina magurska
    podjednostka krynicka
    paleocen
    dolny i środkowy eocen
    litostratygrafia
    tektonika
    Magura Nappe
    Krynica Subunit
    Paleocene
    Lower and Middle Eocene
    lithostratigraphy
    tectonics
    Opis:
    The aim of this work is to present geological structure of the Krynica Subunit of the Magura Nappe in southern part of the Gorce and Beskid Sądecki Mountains and along the Pieniny Klippen Belt (PKB). Formal lithostratigraphic units were distinguished on the basis of mapping. The lithostratigraphic units are the age of Middle Paleocene - Middle Eocene age. Flysch deposits were classified as the Szczawnica Formation with the Życzanów Member, Zarzecze Formation and Piwniczna Member of the Magura Formation in the investigated area. Strike-slip faults and faulted formations complicate the geological structure of the described region. The creation some of these faults is connected with andesite intrusions. Strong faulted strata occurs in the southern part of the region along the overthrust of the Pieniny Klippen Belt on the Magura Nappe. The contact line between the Pieniny Klippen Belt and Paleogene of the Magura Nappe is heterogeneous. The evidences of it are outcrops of klippen, which occur within the Szczawnica Formation and the Zarzecze Formation
    Główny założeniem niniejszej pracy jest zaprezentowanie budowy geologicznej podjednostki krynickiej płaszczowiny magurskiej w południowej części Gorców i Beskidu Sądeckiego oraz na kontakcie z pienińskim pasem skałkowym. Wydzielone na podstawie badań kartograficznych formalne jednostki litostratygraficzne obejmują osady od środkowego paleocenu do środkowego eocenu. Na badanym obszarze osady fliszowe zostały sklasyfikowane jako formacja szczawnicka z ogniwem z Życzanowa, formacja z Zarzecza oraz ogniwo piaskowców z Piwnicznej formacji magurskiej. Budowę geologiczną opisywanego obszaru komplikują liczne uskoki o charakterze przesuwczym oraz sfałdowane utwory fliszowe. Powstanie części tych uskoków jest związane z intruzjami andezytowymi. Mocno sfałdowane utwory występują w południowej części obszaru wzdłuż nasunięcia pienińskiego pasa skałkowego na płaszczowinę magurską. Linia kontaktu pasa skałkowego z paleogenem magurskim nie jest jednolita. Świadczą o tym wychodnie skałek w obrębie utworów formacji szczawnickiej i formacji z Zarzecza
    Źródło:
    Geologia / Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie; 2005, 31, 1; 127-144
    0138-0974
    Pojawia się w:
    Geologia / Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Paramecium tredecaurelia: A Unique Non-Polymorphic Species of the P. aurelia spp. Complex (Oligohymenophorea, Ciliophora)
    Autorzy:
    PRZYBOŚ, Ewa
    Tarcz, Sebastian
    Surmacz, Marta
    Sawka, Natalia
    Fokin, Sergei I.
    Powiązania:
    https://bibliotekanauki.pl/articles/763419.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    P. aurelia species complex, intraspecific polymorphism, rDNA (ITS1-5.8S- ITS2-5’LSU rDNA), cytochrome c oxidase subunit I gene (COI), cytochrome b gene (CytB)
    Opis:
    New stands of Paramecium tredecaurelia, a rare species of the P. aurelia spp. complex, were identified in Thailand and Madagascar on the basis of mating reactions and molecular markers (rDNA and mtDNA). Analysis of DNA fragments showed that all P. tredecaurelia strains, the recently recorded ones and the ones known previously from France, Mexico, and Israel, form a monophyletic and well-defined clade in the P. aurelia species trees. All of these strains, collected from different localities around the world, represent identical or nearly identical haplotypes in terms of all the studied DNA fragments. Given the huge distances between particular collection sites, such a low level of variability of the studied sequences may result from a slow rate of evolution in P. tredecaurelia.
    Źródło:
    Acta Protozoologica; 2013, 52, 4
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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