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Wyszukujesz frazę "spermatozoa" wg kryterium: Temat

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    Wyświetlanie 1-13 z 13
    Tytuł:
    A method for tracing exogenous DNA uptake in live spermatozoa and embryos
    Autorzy:
    Mu, Y.
    Wang, J.
    Liu, Z.
    Zhao, Y.
    Zhang, X.
    Jiao, M.
    Lv, J.
    Hao, J.
    Kong, Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087751.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    spermatozoa
    SMGT
    exogenous DNA
    IVF
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 1; 193-202
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of different egg yolk sources on dog semen quality following cryopreservation
    Autorzy:
    Strzeżek, R.
    Reksa, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539109.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    dog
    semen
    spermatozoa
    extender
    egg yolk
    Opis:
    The aim of this study was to compare the cryoprotective effects of egg yolk from different avian species (hen, goose and quail) on post-thaw quality of dog semen. Total motility (TMOT) and progressive motility (PMOT) of frozen-thawed spermatozoa were not significantly differed among the extenders, but were higher in the quail-egg yolk based extender compared with extender containing hen or goose egg yolk. It was found that post-thaw sperm motion parameters, velocity VCL and ALH, were significantly higher in the quail-egg yolk based extender. No marked differences in post-thaw sperm plasma membrane integrity (PMI) and mitochondrial membrane potential (MMP) were observed among the extenders. In conclusion, the results of the present study suggest that goose or quail egg yolk is a suitable alternative to hen egg yolk for the cryopreservation of dog semen.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 1; 187-189
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Total RNA quality in boar spermatozoa with different freezability
    Autorzy:
    Fraser, L.
    Brym, P.
    Mogielnicka-Brzozowska, M.
    Wasilewska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087601.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    boar
    spermatozoa
    semen freezability
    RNA profiles
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 1; 181-185
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identification and characterisation of mitochondrial proteins isolated from rabbit epididymal spermatozoa – a preliminary study
    Autorzy:
    Lecewicz, M.
    Kordan, W.
    Górski, K.
    Kondracki, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/16648003.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    spermatozoa
    mitochondria
    oxidative stress
    proteins profile
    Opis:
    This is the first study to identify 23 protein spots corresponding to 13 proteins in mitochondria isolated from rabbit epididymal spermatozoa. In the group of protein spots identified in stress-induced samples, the abundance of 20 protein spots increased, whereas the abundance of three protein spots (GSTM3, CUNH9orf172, ODF1) decreased relative to the control. The results of this study provide valuable inputs for future research into the molecular mechanisms implicated in pathological processes during oxidative stress (OS).
    Źródło:
    Polish Journal of Veterinary Sciences; 2023, 26, 2; 311-314
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of bovine follicular fluid on thawed bovine spermatozoa – assessment by CASA system and flow cytometry
    Autorzy:
    Mrowiec, J.
    Twardoń, J.
    Bartoszewicz, A.
    Niżański, W.
    Ochota, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087540.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    bovine follicular fluid
    spermatozoa
    CASA
    flow cytometry
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 2; 305-312
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Relation between nitric oxide (NO) level in semen and certain properties of boar spermatozoa stored at 17°C
    Autorzy:
    Orzołek, A.
    Zasiadczyk, Ł.
    Wysocki, P.
    Kordan, W.
    Krysztofiak, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087744.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    nitric oxide
    liquid storage
    boar spermatozoa quality
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 2; 423-426
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dietary supplementation with algae and polyphenols in rabbit male: Effects on semen quality traits
    Autorzy:
    Vizzarri, Francesco
    Palazzo, Marisa M.
    Casamassima, Donato
    Corino, Carlo
    Chiapparini, Sara
    Ondruska, Lubomir
    Knizatova, Nikola
    Massanyi, Martin
    Tirpak, Filip
    Massanyi, Peter
    Powiązania:
    https://bibliotekanauki.pl/articles/2183570.pdf
    Data publikacji:
    2018-12-31
    Wydawca:
    Uniwersytet Pedagogiczny im. Komisji Edukacji Narodowej w Krakowie
    Tematy:
    extract
    rabbit
    spermatozoa
    mobility
    seminal plasma
    antioxidants
    Opis:
    In recent years, many studies have been focused on natural substances that can affect health of animals. A mix of different extracts was used as dietary supplement and it consists exclusively of natural products. Its main components are polyphenols from terrestrial and marine origins and plant polysaccharides. The effect of this supplement on reproduction has not been reviewed in the past what is a reason why its effect on the reproduction potential of male rabbits was tested. The aim of the present study is to determine effects of natural mix during 120-days long in vivo experiment on selected reproductive traits of male rabbits. Natural mix was supplemented in two different concentrations (T1 - 0.3% and T2 - 0.6%) with the basal ingredients of the conventional rabbit feed in pellet form. In our experiments, emphasis was placed on both the spermatozoa concentration and its motility parameters as well as on the properties of seminal plasma and antioxidant activity. The dietary supplementation with the natural extracts mix positively altered the quality traits of rabbit spermatozoa, but these changes were statistically not significant. In experimental T1 group a significant increase of GPx and FRAP content, both regarding the antioxidant markers profile in seminal plasma was recorded. We can conclude that the supplementation of 0.3% of natural mix did not significantly negatively affect any of the studied reproductive parameters of male rabbits, but some improvement in several antioxidant parameters was found.
    Źródło:
    Annales Universitatis Paedagogicae Cracoviensis Studia Naturae; 2018, 3 (suppl.); 90-97
    2543-8832
    2545-0999
    Pojawia się w:
    Annales Universitatis Paedagogicae Cracoviensis Studia Naturae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Canine post-thaw sperm quality can be predicted by using CASA, and classification and regression tree (CART)-analysis
    Autorzy:
    Schäfer-Somi, S.
    Tichy, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087565.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    dogs
    spermatozoa
    crypreservation
    quality prediction
    CART-analysis
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 1; 51-59
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    High levels of bulky DNA adducts in human sperm correlate with impaired fertility.
    Autorzy:
    Horak, Stanisław
    Polańska, Joanna
    Widłak, Piotr
    Powiązania:
    https://bibliotekanauki.pl/articles/1043666.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    fertility
    spermatozoa
    human
    DNA damage
    adducts
    32P-postlabelling
    Opis:
    Progressive decline in fertility and sperm quality has been reported over the last few decades, especially in industrialized nations. It has been proposed that exposure to factors that induce damage in DNA of spermatogenic cells may significantly contribute to impaired fertility. Here, the 32P-postlabelling method was used to analyze the levels of bulky DNA adducts in sperm cells in a group of 179 volunteers, either healthy subjects or patients with an impaired fertility. The levels of DNA adducts were 1.35-fold higher in the infertile group as compared to healthy individuals (P = 0.012). Similarly, a significant negative correlation between the levels of DNA adducts and measures of semen quality (sperm concentration and motility) has been observed (P Ł 0.001). In addition, the levels of bulky DNA adducts in sperm cells positively correlates with amounts of leukocytes in semen, which were significantly higher in semen of infertile subjects.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 1; 197-203
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zinc-binding proteins from boar seminal plasma - isolation, biochemical characteristics and influence on spermatozoa stored at 4°C
    Autorzy:
    Mogielnicka-Brzozowska, Marzena
    Wysocki, Paweł
    Strzeżek, Jerzy
    Kordan, Władysław
    Powiązania:
    https://bibliotekanauki.pl/articles/1039909.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    boar
    spermatozoa
    zinc
    Zn2+-binding proteins
    cold shock
    Opis:
    Affinity chromatography on Chelating Sepharose Fast Flow Gel-Zn2+ was used for fractionation of boar seminal plasma proteins. Approximately 30% of total boar seminal plasma proteins showed affinity for zinc ions (ZnBP fraction). Native electrophoresis (PAGE) of ZnBP revealed six protein fractions which separated into 27 bands under denaturing conditions (SDS/PAGE). Two-dimensional electrophoresis (2D PAGE) showed 148 polypeptides with isoelectric points mostly in the basic and neutral pH range. The zinc-binding proteins comprise mainly 10-20 kDa polypeptides which are probably members of the spermadhesin family. ZnBP present in the incubation mixture of spermatozoa stored for 1 or 24 h at 4 °C allowed preservation of a higher percentage of cells exhibiting linear motility in comparison to a control sample stored in PBS. Presented results indicate that proteins binding Zn2+ ions have a shielding effect on the sperm plasma membrane and acrosome of spermatozoa, protecting these structures against consequences of cold shock.
    Źródło:
    Acta Biochimica Polonica; 2011, 58, 2; 171-177
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of nitric oxide on boar sperm motility, membrane integrity, and acrosomal status during semen storage
    Autorzy:
    Jovicić, M.
    Pintus, E.
    Fenclova, T.
    Simonik, O.
    Chmelikova, E.
    Ros-Santaella, J. L.
    Sedmikova, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087766.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    boar spermatozoa
    nitric oxide
    nitric oxide synthases
    NO donor
    NOS inhibitor
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 1; 73-82
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Protamine-like proteins analysis as an emerging biotechnique for cadmium impact assessment on male mollusk Mytilus galloprovincialis (Lamarck 1819)
    Autorzy:
    Piscopo, Marina
    Trifuoggi, Marco
    Notariale, Rosaria
    Labar, Sofiane
    Troisi, Jacopo
    Giarra, Antonella
    Rabbito, Dea
    Puoti, Raffaela
    de Benedictis, Domenico
    Brundo, Maria
    Basile, Adriana
    Good, Katrina
    Ausió, Juan
    Guerriero, Giulia
    Powiązania:
    https://bibliotekanauki.pl/articles/1038400.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Mytilus galloprovincialis
    cadmium impact assessment
    emerging biotechniques
    testis metallothionein
    Protamine-like-proteins
    spermatozoa
    Opis:
    Here we report the industrial pollution effects due to cadmium on the reproductive health of Mytilus galloprovincialis. Mussels were removed from the biofouling of a Conatex panel after one year exposition at a polluted site near a disposal metallurgical factory. A high cadmium bioaccumulation was observed in the testis of mussels housed at the polluted site, with respect to a control site, as determined by inductively coupled plasma-mass spectrometry, along with a 10 fold increase in metallothionein 20 kDa gene (mt20) expression levels determined by qPCR. Furthermore, mussels transferred into laboratory tanks from the reference site, and exposed to 1.5, 5 and 10 µM CdCl2, revealed a 1.7, 3.2 and 4.5 fold expression increase in the testis mt20, respectively, and a positive correlation with cadmium bioaccumulation was found. To evaluate a potential detrimental risk of such alterations on spermatozoa, we carried out electrophoretic analyses on their protamine-like proteins. As determined by AU-PAGE, after 1.5 µM CdCl2 exposure, protamine-like proteins also display major alterations with respect to those obtained after 5 and 10 µM CdCl2 exposure. All protamine-like proteins isolated from the polluted biofouling were in an aggregated form and displayed the same reduced DNA binding affinity of the protamine-like proteins obtained after 1.5 µM CdCl2 as demonstrated EMSA with sperm genomic DNA. Our results contribute to the studies concerning cadmium induced testis alterations and highlight protamine-like proteins' analysis as an emerging biotechnique for cadmium impact assessment on Mytilus galloprovincialis, for the sensitivity of the in vivo and in vitro changes of protamine-like proteins' state and their DNA binding affinity.
    Źródło:
    Acta Biochimica Polonica; 2018, 65, 2; 259-267
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of low-frequency electric field screening on motility of human sperm
    Autorzy:
    Górski, R.
    Kotwicka, M.
    Skibińska, I.
    Jendraszak, M.
    Wosiński, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085910.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    human spermatozoa
    male infertility
    50 Hz EMF – 50 Hz electromagnetic field
    dielectric screen
    ADR Technology®
    Opis:
    Introduction. The human body is constantly exposed to an extremely low electromagnetic field (ELF-EMF), in particular at 50 Hz, emitted by power lines, domestic distribution lines, electrical appliances, etc. It is assumed that the increase in electromagnetic exposure may cause adverse effects upon human health, as well as raising concerns regarding the impact on human fertility. Objective. The aim of this in vitro study was to investigate the influence of ELF-EMF with a frequency of 50 Hz on the motility of human sperm. At the same time, the effectiveness of the dielectric screen constructed by ADR Technology® in absorbing the emitted radiation was examined. Materials and method. Semen samples of 20 patients were exposed to the influence of an extremely low electromagnetic field. After 5, 15 and 30 min., spermatozoa motility was analysed using a computer-assisted spermatozoa motility analysis system. The following sperm motility parameters were examined: 1) velocity straight linear motility; 2) cross-beat frequency; 3) lateral head displacement; 4) homogeneity of progressive motility velocity. Results. It was found that the ELF-EMF presented a negative effect on the motility of human spermatozoa. A significant decrease in spermatozoa motility speed and a significant increase in lateral head deviation values were observed under the influence of the electromagnetic field. ELF-EMF did not show an effect on either lateral head displacement or homogeneity of progressive motility velocity. Conclusions. A positive effect of the dielectric screen ADR Technology® was found. This effect compensated spermatozoa motility changes induced with ELF-EMF.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 3; 427-434
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-13 z 13

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