- Tytuł:
- Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori
- Autorzy:
-
Pan, Ye
Xia, Hengchuan
Lü, Peng
Chen, KePing
Yao, Qin
Chen, Huiqin
Gao, Lu
He, Yuanqing
Wang, Lin - Powiązania:
- https://bibliotekanauki.pl/articles/1040486.pdf
- Data publikacji:
- 2009
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
serpin-2
Bombyx mori
bioinformatics
subcellular location
qPCR - Opis:
- Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.
- Źródło:
-
Acta Biochimica Polonica; 2009, 56, 4; 671-677
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł