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Wyszukujesz frazę "scanning microscopy" wg kryterium: Temat


Tytuł:
ARMScope – the versatile platform for scanning probe microscopy systems
Autorzy:
Świadkowski, Bartosz
Piasecki, Tomasz
Rudek, Maciej
Świątkowski, Michał
Gajewski, Krzysztof
Majstrzyk, Wojciech
Babij, Michał
Dzierka, Andrzej
Gotszalk, Teodor
Powiązania:
https://bibliotekanauki.pl/articles/221551.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Scanning probe microscopy
AFM
Kelvin Probe force microscopy
scanning tunnelling microscopy
Opis:
Scanning probe microscopy (SPM) since its invention in the 80’s became very popular in examination of many different sample parameters, both in university and industry. This was the effect of bringing this technology closer to the operator. Although the ease of use opened a possibility for measurements without high labour requirement, a quantitative analysis is still a limitation in Scanning Probe Microscopes available on the market. Based on experience of Nano-metrology Group, SPM still can be considered as a tool for quantitative examination of thermal, electrical and mechanical surface parameters. In this work we present an ARMScope platform as a versatile SPM controller that is proved to be useful in a variety of applications: from atomic-resolution STM (Scanning Tunnelling Microscopy) to Multi-resonance KPFM (Kelvin Probe force microscopy) to commercial SEMs (Scanning electron microscopes).
Źródło:
Metrology and Measurement Systems; 2020, 27, 1; 119-130
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Novel luminescent dyes for confocal laser scanning microscopy used in Trematoda parasite diagnostics
Autorzy:
Kirilova, Elena
Kecko, Sanita
Mežaraupe, Ligita
Gavarāne, Inese
Pučkins, Aleksandrs
Mickeviča, Ilona
Rubeniņa, Ilze
Osipovs, Sergejs
Bulanovs, Andrejs
Pupiņš, Mihails
Kirjušina, Muza
Powiązania:
https://bibliotekanauki.pl/articles/1038378.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
benzanthrone dyes
trematode
confocal laser scanning microscopy
Opis:
Benzanthrone derivates are now widely used in many industrial and scientific applications as dyes for polymers and textiles. In biochemical, biomedical and diagnostics investigations benzanthrone dyes are used as a lipophilic fluorescent probe since many benzanthrone derivates demonstrate bright fluorescence and they have ability to intercalate between membrane lipids. The aim of research presented here was to assess the luminescence ability of benzanthrone derivatives using microscopic visualization of biological objects. Accordingly, specimens of freshwater trematodes: Diplostomum spathaceum, Diplodiscus subclavatus and Prosotocus confusus, were stained by novel benzanthrone dyes using different fixatives. The samples were examined under a confocal laser scanning microscope. All of the dyes tested demonstrated good results for digestive and reproductive system visualization. Based on obtained results we conclude that benzanthrone dyes could be used for internal and external structure confocal laser scanning microscopic imaging of trematode specimens.
Źródło:
Acta Biochimica Polonica; 2018, 65, 3; 449-454
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie cienkich warstw metali i ich tlenków w preparatyce próbek do badań z zastosowaniem skaningowej mikroskopii elektronowej
The application of thin transparent metal layers and their oxides in preparation of biological samples for scanning electron microscopy
Autorzy:
Grudniewski, T.
Chodyka, M.
Czernik, S.
Plewik, D.
Powiązania:
https://bibliotekanauki.pl/articles/251686.pdf
Data publikacji:
2015
Wydawca:
Instytut Naukowo-Wydawniczy TTS
Tematy:
sputtering magnetronowy
mikroskopia skaningowa
mikroskopia elektronowa
scanning microscopy
electron microscopy
Opis:
Skaningowa mikroskopia elektronowa wymaga aby próbka była przewodząca, lub pokryta warstwą przewodzącego materiału. Pomimo tego że złoto jest metalem najczęściej używanym do pokrywania próbek, jest także bardzo drogie, więc autorzy postanowili sprawdzić czy inne metale lub stopy metali będą nadawać się do pokrywania próbek. Próbki były pokrywane w procesie sputteringu magnetronowego przy pomocy urządzenia LINE 440 (Alliance Concept), a obserwacje przeprowadzone na mikroskopie Hitachi TM3000. Próbki były pokryte miedzią, stopem miedzi i galu, cyną i tlenkiem cyny. Obrazy otrzymane w czasie eksperymentu były porównywalne z próbkami pokrytymi złotem.
Paper discussed the impact of alcohol on the psychophysical properties of human and its metabolism. Particular attention was paid to the influence of alcohol on driving ability. Authors proposed action to be taken to reduce.
Źródło:
TTS Technika Transportu Szynowego; 2015, 12; 620-626, CD
1232-3829
2543-5728
Pojawia się w:
TTS Technika Transportu Szynowego
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation and vizualization of the cells grown on ceramic coating by electron microscopy techniques
Autorzy:
Karbowniczek, J.
Gruszczyński, A.
Kruk, A
Czyrska-Filemonowicz, A.
Powiązania:
https://bibliotekanauki.pl/articles/285222.pdf
Data publikacji:
2017
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
biocompatibility
biomaterials
scanning electron microscopy
Źródło:
Engineering of Biomaterials; 2017, 20, no. 143 spec. iss.; 37
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of Iota-Carrageenan and Its Hydrolysates on the Stability of Milk Ice Cream Mixes
Autorzy:
Kot, Anna
Jakubczyk, Ewa
Buniowska-Olejnik, Magdalena
Kamińska-Dwórznicka, Anna
Powiązania:
https://bibliotekanauki.pl/articles/16538547.pdf
Data publikacji:
2023-06-02
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
ice cream mix emulsion
particle size
confocal laser scanning microscopy
Opis:
The objective of this research was to determine the influence of iota-carrageenan and its acid and enzymatic hydrolysates on the physical properties of milk ice cream mixes. The main factors considered were the Turbiscan stability index, backscattering profile, particle size distribution and median diameter (D50), the consistency index and the flow behavior index of ice cream mix before and after 24 h of maturation at 4°C. The microstructure of emulsion was also analysed based on confocal laser scanning microscopy (CLSM). The addition of iota-carrageenan resulted in lower stability of emulsion compared to emulsions with its acid and enzymatic hydrolysates. The sedimentation, coalescence and flocculation were observed based on the backscattering profile and CLSM images. The addition of stabilisers contributed to an increase in D50 of ice cream mix from 17.56 to 37.05–45.50 µm before maturation and from 34.73 to 46.73 µm after maturation. The iota-carrageenan after commercial lactase treatment improved the stability of milk ice cream mixes by increasing the consistency index to 0.104 and a flow behaviour index to 0.702. Finally, it may be concluded that the stabilisers used – iota-carrageenan and its hydrolysates – significantly influenced the physical properties of milk ice cream mixes and, hence, can be used as beneficial ingredients in the recipe of milk ice cream mixes.
Źródło:
Polish Journal of Food and Nutrition Sciences; 2023, 73, 2; 196-204
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Use of scanning electron microscopy for the study of Hoplopleura (Phthiraptera, Anoplura) lice taxonomy
Autorzy:
Kozina, P.
Izdebska, J.N.
Mierzynski, L.
Powiązania:
https://bibliotekanauki.pl/articles/5924.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
scanning electron microscopy
Hoplopleura
Phthiraptera
Anoplura
taxonomy
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prospective observational study of adenoidal biofilms in a paediatric population and its clinical implications
Autorzy:
Subtil, Joao
Bajanca-Lavado, Maria Paula
Rodrigues, Joao
Duarte, Aida
Reis, Lucia
Nogueira, Isabel
Jordao, Luisa
Powiązania:
https://bibliotekanauki.pl/articles/1397787.pdf
Data publikacji:
2019
Wydawca:
Index Copernicus International
Tematy:
Adenoids
biofilms
scanning electron microscopy
Haemophilus
child.
Opis:
INTRODUCTION: Adenoids are nasopharyngeal lymphoid tissue with a relevant role in host defence against infection of upper respiratory tract. Nevertheless, adenoids are also a reservoir of microorganisms that can cause infections of upper respiratory tract and otitis particularly in children. OBJECTIVE: Evaluate and compare the association between biofilm assembly on adenoids and the incidence of recurrent infections in a paediatric population submitted to adenoidectomy by either infectious or non-infectious indication. METHODS: Scanning electron microscopy was used to assess biofilms on adenoid surface; biofilm assembly in vitro was monitored by crystal violet assay; antibiotic susceptibility was assessed following EUCAST guidelines; Hinfluenzae capsular typing was performed by PCR. RESULTS: Biofilms were present in 27.4% of adenoid samples and no statistical difference was found between infectious and non-infectious groups. In vitro, the most clinically relevant bacteria, H.influenzae, S.aureus, S.pyogenes, S.pneumoniae and M.catarrhalis, were mostly moderate biofilm assemblers (71.7%). 55.3% of these bacteria were intermediate/resistant to at least one of the tested antibiotics. No association was found between the ability to assemble biofilms in vitro and the presence of biofilms on adenoids nor antibiotic resistance. All H.influenzae were characterized as non-typeable. CONCLUSION: The presence of biofilms on adenoid surface was independent from clinical sample background. Bacterial ability to assemble biofilms in vitro cannot be used to predict biofilm assembly in vivo. The lack of correlation between biofilm formation and infectious respiratory diseases found contributes to question the relevance of biofilms on the pathogenesis of infectious diseases.
Źródło:
Polish Journal of Otolaryngology; 2019, 73, 1; 22-28
0030-6657
2300-8423
Pojawia się w:
Polish Journal of Otolaryngology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Anatomical study on the developing pericarp of selected Rosa species (Rosaceae)
Autorzy:
Guzicka, M.
Zielinski, J.
Tomaszewski, D.
Gawlak, M.
Powiązania:
https://bibliotekanauki.pl/articles/41015.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
plant anatomy
developing pericarp
pericarp
structure
wild plant
rose
Rosa
Rosaceae
SEM analysis
CLSM zob.confocal laser scanning microscopy
confocal laser scanning microscopy
lignification
achene
Opis:
Results of anatomical studies on the developing pericarp of selected wild roses are presented. Using SEM and CLSM, the changes in the pericarp structure of 5 species have been observed during its formation, from the flowering stage to fully ripe achenes. In the morphological development of the pericarp of Rosa species two main phases can be distinguished: the phase of intensive growth of the pericarp during which the fruit achieves its final shape and volume, and the subsequent phase of pericarp ripening when no significant morphological changes in the pericarp occur. Similarly, in the process of the anatomical development of the pericarp two phases are noticeable, however, during both stages, great internal changes proceed in the fruit. The first phase consists of intensive cell divisions and enlargement, gradual thickening of cell walls and formation of all pericarp layers. Due to these changes, the pericarp achieves its final anatomical structure. The second phase, involving the pericarp ripening, is manifested in the modification of cell walls, mainly by their quick thickening, but first of all by their lignification. The lignification of pericarp cell walls begins in the inner endocarp; it proceeds in the outer endocarp, later in mesocarp and finishes in the hypodermal cells of the exocarp. The epidermal cells remain alive the longest and their walls do not (or hardly) become lignified. The death of all cells finishes the pericarp ripening.
Źródło:
Dendrobiology; 2012, 68
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Deformation Mechanisms and Fracture of Ni-Based Metallic Glasses
Autorzy:
Lesz, S.
Griner, S.
Nowosielski, R.
Powiązania:
https://bibliotekanauki.pl/articles/353790.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
metals
transmission electron microscopy
scanning electron microscopy
fracture
shear bands
Opis:
The cracking of materials and fracture surface is of great practical and academic importance. Over the last few years the development of the fractography of crystalline alloys resulted in a useful tool for the prediction or failure analysis. Many attempts have been made to observe cracks using optical microscopy, X-ray topography and transmission electron microscopy (TEM). Of these techniques, the resolution of optical microscopy and X-ray topography is too poor. By contrast, the resolution of TEM is high enough for detailed information to be obtained. However, in order to apply TEM observations, a thin foil specimen must be prepared, and it is usually extremely difficult to prepare such a specimen from a pre-selected region containing a crack. In the present work, deformation mechanisms fracture surfaces of Ni-based metallic glass samples have been studied by specially designed experiments. In order to study the deformation mechanisms and fracture the Ni-based metallic glasses have been investigated in the tensile test. The structure and fracture surfaces after the decohesion process in tensile tests were observed using transmission electron microscope (TEM) and scanning electron microscope (SEM), respectively. The studies of structure were performed on thin foils. Moreover the investigated tape was subjected to a banding test. Then, the tape was straightened and the thin foil from the area of maximum strain was prepared. This thin foil sample was deformed before the TEM investigation to obtain local tears.
Źródło:
Archives of Metallurgy and Materials; 2016, 61, 2A; 791-796
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie mikroskopii AFM do wizualizacji efektów prac nad otrzymaniem tlenków cyny SNO2
The SNO2 formation proces observation and visualisation using AFM
Autorzy:
Lubańska, Z.
Grudniewski, T.
Chodyka, M.
Powiązania:
https://bibliotekanauki.pl/articles/252767.pdf
Data publikacji:
2015
Wydawca:
Instytut Naukowo-Wydawniczy TTS
Tematy:
mikroskopia (AFM)
SnO2
mikroskopia skaningowa
mikroskopia atomowa
STM
AFM
AFM microscopy
scanning microscopy
atomic microscopy
Opis:
W artykule przedstawiono charakterystyki cienkich warstw SnO2 na czystym szkle. Warstwy SnO2 nanoszone były metodą magnetronowego sputteringu (nanoszenie) na podłoże szklane w różnych temperaturach. Wykorzystano do tego urządzenie Magnetron Line 440. Do badania składu i morfologii warstwy użyto mikroskopii skaningowej i atomowej (STM/AFM). Na podstawie badań stwierdzono, że powłoki wykazują dobre połączenie z materiałem podłoża, charakteryzują się zróżnicowaną chropowatością i są jednorodnie chemiczne.
Atomic force microscopy is one of the most popular method used in surface imaging. This method allows to measure the surface topography and determine the dimensions of the structures in the subatomic resolution [1]. Due to its properties, it can be applied to the measurement of conductors and semiconductor surfaces prepared in various processes. The experiment is fo-cused on SnO2 and ITO thin layers which can be used as transparent electrodes [2]. The authors are trying to illustrate the correlation between process parameters - creation of semiconductor in magnetron sputtering by different process conditions (temperature and cooling process, gas pressure and composition), surface of the sample and its other electrooptical parameters.
Źródło:
TTS Technika Transportu Szynowego; 2015, 12; 944-946, CD
1232-3829
2543-5728
Pojawia się w:
TTS Technika Transportu Szynowego
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diversity of labellar micromorphological structures in selected species of Malaxidinae [Orchidales]
Autorzy:
Kowalkowska, A
Margonska, H.B.
Powiązania:
https://bibliotekanauki.pl/articles/58622.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
light microscopy
scanning electron microscopy
Orchidaceae
micromorphology
Malaxidinae
pollination
Opis:
There are no papers examining the labellar micromorphology in Malaxidinae in detail. The aim of this paper is to present the results of labellar micromorphology of our studies on Malaxidinae. The micromorphology of nine species was examined and described (calli, nectaries, OP structures, concavities). These species are divided into six groups of which the lip morphology probably represents different forms of attracting and rewarding pollinators. Group one consists of Disticholiparis gregaria and Platystyliparis aurita with characteristic globular nectary and pad-like callus. The second group with Liparis nervosa has two conelike convexities. The third one is represented by Liparis sutupensis with spread lip, the basal callus, and the epichile often eroding with age. The fourth group (Stichorkis cespitosum) has a massive hypochil with basal callus and delicate epichil. The fifth group (Microstylis ophioglossoides, Dienia ophrydis) is characterized by a lip with developed lateral lobes, three deep concavities in the middle part, an erect callus, and many raphide cells.Crepidium cf. rheedii and Crepidium dryadum from the sixth group have a three-lobed lip with entire or toothed mid-lobe, basal callus, cavity surrounded by a rim and OP structures with raphide cells. We also emphasized the different way and place of nectar secretion in the subtribe and gave some suggestions about pollination strategies.
Źródło:
Acta Societatis Botanicorum Poloniae; 2009, 78, 2; 141-150
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ wybranych substancji chemicznych na degradację tworzyw poliolefinowych
Impact of selected chemical substances on the degradation of the polyolefin materials
Autorzy:
Czop, M.
Biegańska, J.
Powiązania:
https://bibliotekanauki.pl/articles/1208029.pdf
Data publikacji:
2012
Wydawca:
Stowarzyszenie Inżynierów i Techników Przemysłu Chemicznego. Zakład Wydawniczy CHEMPRESS-SITPChem
Tematy:
polimery
degradacja
substancje chemiczne
mikroskopia skaningowa
degradation
polymers
chemical substances
scanning microscopy
Opis:
Zwiększająca się produkcja i konsumpcja różnego rodzaju produktów z tworzyw sztucznych prowadzi do zwiększenia strumienia odpadów, w którym przeważającą część stanowią odpady z grupy poliolefin. Z powodu braku podatności na hydrolizę czy dekompozycję, unieszkodliwianie większości odpadów z tworzyw sztucznych stanowi skomplikowany problem. Rosnąca ilość zużytych odpadów z tworzyw sztucznych wymusza poszukiwanie nowych, ekologicznych i bezpiecznych metod ich zagospodarowania. Tworzywa sztuczne są uznawane obecnie za główne zanieczyszczenie środowiska przyrodniczego, ale przekształcone w odpowiedni sposób mogą stanowić cenny surowiec produkcyjny. Recykling odpadowych polimerów jest jednym z perspektywicznych kierunków ich wykorzystania, prowadząc jednocześnie do ograniczenia zużycia surowców naturalnych. Przy kontakcie z gazami, cieczami i ciałami stałymi, substancje chemiczne mogą wnikać do wnętrza tworzywa sztucznego lub wypłukiwać z niego różne składniki. Odporność tworzywa sztucznego na wpływ substancji chemicznych określa się mianem odporności lub podatności chemicznej. Oddziaływanie substancji chemicznych na tworzywa sztuczne zależny od wielu czynników. W artykule zaprezentowano wyniki badań określające stopień odporności na degradację tworzyw poliolefinowych z zastosowaniem nieorganicznych substancji chemicznych. Stopień degradacji badanych odpadów określono poprzez rejestrację zmiany masy oraz zmian makroskopowych i mikroskopowych ich struktury.
An increased production and consumption of various types of plastics leads to the increase of the wastes. The majority of wastes are formed by wastes from polyolefin group. Due to lack of susceptibility to hydrolysis or decomposition, utilization of the plastic wastes is a very complicated problem. Increasing amount of plastic wastes forces a search for the new, ecological and safe methods of waste management. Nowadays, plastics are considered to be main pollution of the natural environment. However, modified plastics can constitute a valuable production material. Recycling of waste polymers is one of the far-reaching methods of their utilization and at the same time limits the consumption of the natural resources. When having contacts with gases, liquids and solids, chemical substances may penetrate into the plastic or wash out various components from them. Resistance of plastics to the impact of chemical substances is called chemical resistance or susceptibility. Impact of chemical substances on plastics depends on many factors. The article will present research results which will define the grade of resistance to degradation of polyolefin materials with the use of non-organic chemical substances. The degree of degradation of the waste tests has been defined through the registration of the weight change as well as macroscopic and microscopic changes of their structure.
Źródło:
Chemik; 2012, 66, 4; 307-314
0009-2886
Pojawia się w:
Chemik
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Degradacja odpadów poliolefinowych wybranymi substancjami chemicznymi
Studying the impact of benzene, chloroform and xylene on the decomposition of plastic
Autorzy:
Bogacka, M.
Czop, M.
Gan, K.
Sadowska, K.
Powiązania:
https://bibliotekanauki.pl/articles/357577.pdf
Data publikacji:
2014
Wydawca:
Politechnika Śląska
Tematy:
polimery
degradacja
substancje chemiczne
mikroskopia skaningowa
polymers
degradation
chemical substances
scanning microscopy
Opis:
W artykule przedstawiono wyniki badań dotyczące degradacji tworzyw sztucznych w środowisku chemicznym. Do pomiaru zastosowano tworzywa sztuczne z grupy poliolefin. Badany materiał został poddany obróbce mechanicznej, a następnie w zadanej ilości zanurzony w ciekłych chemikaliach. Część prób została wystawiona na działanie promieniowania słonecznego, a pozostałe umieszczono w miejscu bez dostępu promieniowania UV. Zmiany masy były rejestrowane zgodnie z obowiązującymi normami. Celem eksperymentu było znalezienie czynników chemicznych mogących naruszyć strukturę powierzchni tworzyw sztucznych oraz określenie odporności tworzyw poliolefinowych za czynniki chemiczne.
The article presents the results of studies concerning the degradation of plastics in the chemical environment. Plastics in the group of polyolefins were used for measurement. The examinated material has been subjected to mechanical treatment, and then immersed in the required amount of liquid chemicals. Some attempts have been exposed to solar radiation, and the others placed in a location without access to UV radiation. Changes of weight were recorded in accordance with valid standards. The purpose of this experiment was to find factors that may affect the chemical structure of plastics.
Źródło:
Archiwum Gospodarki Odpadami i Ochrony Środowiska; 2014, 16, 1; 59-68
1733-4381
Pojawia się w:
Archiwum Gospodarki Odpadami i Ochrony Środowiska
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microscopic analysis of the nanostructures impact on endothelial cells
Autorzy:
Kołodziejczyk, Agnieszka Maria
Kucińska, Magdalena
Jakubowska, Aleksandra
Siatkowska, Małgorzata
Sokołowska, Paulina
Kotarba, Sylwia
Makowski, Krzysztof
Komorowski, Piotr
Walkowiak, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/1844983.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
nanostructures
atomic force microscopy
transmission electron microscopy
scanning electron microscopy
cell morphology
Opis:
Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
Źródło:
Engineering of Biomaterials; 2020, 23, 154; 2-8
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microscopic analysis of the nanostructures impact on endothelial cells
Autorzy:
Kołodziejczyk, Agnieszka Maria
Kucińska, Magdalena
Jakubowska, Aleksandra
Siatkowska, Małgorzata
Sokołowska, Paulina
Kotarba, Sylwia
Makowski, Krzysztof
Komorowski, Piotr
Walkowiak, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/970974.pdf
Data publikacji:
2020
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
nanostructures
atomic force microscopy
transmission electron microscopy
scanning electron microscopy
cell morphology
Opis:
Nowadays nanostructures are more and more often designed as carriers for drug delivery, especially to improve the drug pharmacokinetics and pharmaco-dynamics. Numerous kinds of nanostructures are considered a good prospect for medical applications thanks to their small size, acceptable biocompatibility and toxicity. Due to the fact that nanotechnology is a new field of science, every nano-scale product must be thoroughly examined regarding its toxicity to the human body. This study provides new insights into effects of exposing endothelial cells to the selected nanostructures. Dendrimers of the fourth generation (PAMAMs), multi-walled carbon nanotubes (MWCNTs) and silver nanoparticles (SNPs) were used to evaluate nanostructures influence on endothelial cells in vitro. The nanostructures were evaluated via transmission electron microscopy and dynamic light scattering technique. The cells previously exposed to the nanostructures were observed and analyzed via the atomic force microscopy and scanning electron microscopy to obtain a quantitative evaluation of the cells morphology. The presence of multi-walled carbon nanotubes and silver nanoparticles on the cells surface was confirmed by the scanning electron microscopy. Our results confirm that the surface association and/or uptake of nanostructures by the cells resulting from physicochemical and biological processes, affect the cells morphology. Morphological changes can be induced by the membrane proteins interaction with nanomaterials, which trigger a sequence of intracel-lular biological processes.
Źródło:
Engineering of Biomaterials; 2020, 23, 154; 2-8
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł

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