Temat: real-time polymerase chain reaction - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: The influence of LDHA gene polymorphism on relative level of its expression in racing pigeons
Polimorfizm w genie LDHA i jego wpływ na względny poziom ekspresji u gołębi pocztowych
Autorzy:: Jędrzejczak-Silicka, M.
Yu, Y.-H.
Cheng, Y.-H.
Dybus, A.
Powiązania:: https://bibliotekanauki.pl/articles/2606361.pdf
Data publikacji:: 2018
Wydawca:: Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:: gene polymorphism
gene expression
pigeon
real-time polymerase chain reaction
Źródło:: Acta Scientiarum Polonorum. Zootechnica; 2018, 17, 3; 9-15
1644-0714
Pojawia się w:: Acta Scientiarum Polonorum. Zootechnica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: SYBR Green-based real-time polymerase chain reaction assay for detection of porcine parvovirus 6 in pigs
Autorzy:: Sun, P.
Bai, C.X.
Zhang, D.
Wang, J.
Yang, K.K.
Cheng, B.Z.
Li, Y.D.
Wang, Y.
Powiązania:: https://bibliotekanauki.pl/articles/2087328.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: porcine parvovirus 6
real-time polymerase chain reaction
SYBR Green
Źródło:: Polish Journal of Veterinary Sciences; 2020, 23, 2; 197-202
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Quantification of thioredoxin mRNA expression in the rat hippocampus by real-time PCR following oxidative stress.
Autorzy:: Yalcin, Ayfer
Powiązania:: https://bibliotekanauki.pl/articles/1041524.pdf
Data publikacji:: 2004
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: kainic acid
real-time polymerase chain reaction
oxidative stress
thioredoxin
Opis:: Thioredoxin (Trx) is a multifunctional protein with a redox-active disulfide/dithiol in the active site. Thioredoxin, with its redox-regulating and reactive oxygen species (ROS) scavenging activities, plays several important biologic roles both in intracellular and extracellular compartments. The purpose of this report was to quantify the relative expression of Trx in rat hippocampus following an oxidative stress-involving treatment such as kainic acid (KA) using real-time PCR and the 2-ΔΔCT method. The relative changes in expression of Trx mRNA in KA-treated and control animals were significantly different as 2.02 ± 0.77 and 1.0 ± 0.26, respectively ( P <0.05). Minimum and maximum n-fold changes in Trx expression in KA-treated and control animals were determined as (1.4-5.2) and (0.8-1.3), respectively. Thus, real-time PCR and the 2-ΔΔCT method for data analysis from real-time PCR were found to be an accurate and sensitive method for quantifying Trx mRNA levels.
Źródło:: Acta Biochimica Polonica; 2004, 51, 4; 1059-1065
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Quantification of thioredoxin mRNA expression in the rat hippocampus by real-time PCR following oxidative stress.
Autorzy:: Yalcin, Ayfer
Powiązania:: https://bibliotekanauki.pl/articles/1041529.pdf
Data publikacji:: 2004
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: kainic acid
real-time polymerase chain reaction
oxidative stress
thioredoxin
Opis:: Thioredoxin (Trx) is a multifunctional protein with a redox-active disulfide/dithiol in the active site. Thioredoxin, with its redox-regulating and reactive oxygen species (ROS) scavenging activities, plays several important biologic roles both in intracellular and extracellular compartments. The purpose of this report was to quantify the relative expression of Trx in rat hippocampus following an oxidative stress-involving treatment such as kainic acid (KA) using real-time PCR and the 2-ΔΔCT method. The relative changes in expression of Trx mRNA in KA-treated and control animals were significantly different as 2.02 ± 0.77 and 1.0 ± 0.26, respectively ( P <0.05). Minimum and maximum n-fold changes in Trx expression in KA-treated and control animals were determined as (1.4-5.2) and (0.8-1.3), respectively. Thus, real-time PCR and the 2-ΔΔCT method for data analysis from real-time PCR were found to be an accurate and sensitive method for quantifying Trx mRNA levels.
Źródło:: Acta Biochimica Polonica; 2004, 51, 4; 1087-1090
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Construction of new GFP-tagged fusants for Trichoderma harzianum with enhanced biocontrol activity
Autorzy:: Kowsari, M.
Motallebi, M.
Zamani, R.M.
Powiązania:: https://bibliotekanauki.pl/articles/66878.pdf
Data publikacji:: 2014
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: biomonitor
construction
Trichoderma harzianum
biological control
green fluorescent protein
real-time polymerase chain reaction
Opis:: Trichoderma is one of the most exploited biocontrol agents for the management of plant diseases. In biocontrol ecology, the critical factors are detection, and the monitoring and recovery of specific biocontrol agents either naturally present or deliberately released into the environment. Protoplast fusion is an appropriate tool for the improvement of biocontrol Trichoderma strains. Protoplast isolation from Trichoderma harzianum was achieved using 24 h culture age, 6.6 mg/ml Novazym L 1412 at 30°C which resulted the maximum protoplast yield of 5 × 108/ml. The self-fused protoplasts were regenerated and 12 fusants were selected based on their growth rate on 2% colloidal chitin medium. Next, a comparison was done for chitinase and antagonistic activity. Transcriptomic analysis based on quantitative real-time RT-PCR, demonstrated that T8-05 fusant expressed 1.5 fold of chit42 transcript as compared with the parental line. This fusant with 7.02±0.15U chitinase activity showed a higher growth inhibition rate (100%) than the parent strain, against Rhizoctonia solani. To obtain a genetically marked fusant that can be used as a biomonitor, the fusant was cotransformed with the gfp and amdS genes. The morphology and viability of selected cotransformant (FT8-7MK-05-2) was similar to the parent. Green fluorescing conidia were observed within the first 2 days of incubation in the soil, and this was followed by the formation of chlamydopores after 60 days. The colonisation of the gfp-tagged fusant was also monitored visually on R. solani sclerotia by scanning electron microscopy. Production of gfp-tagged fusant of Trichoderma spp. provides a potentially useful tool for monitoring hyphal growth patterns and the population of biocontrol agent isolates introduced into environmental systems.
Źródło:: Journal of Plant Protection Research; 2014, 54, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: HybProbes-based real-time PCR assay for rapid detection of equine herpesvirus type 2 DNA
Autorzy:: Osinska, E.
Golke, A.
Slonska, A.
Cymerys, J.
Banbura, M.W.
Dzieciatkowski, T.
Powiązania:: https://bibliotekanauki.pl/articles/30252.pdf
Data publikacji:: 2012
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: rapid detection
equine herpesvirus
real-time polymerase chain reaction
horse
herpesvirus
Gammaherpesvirinae
Rhadinovirus
veterinary virology
Opis:: Equid herpesvirus type 2 (EHV-2) together with equid herpesvirus type 5 are members of Gammaherpesvirinae subfamily, genus Rhadinovirus. EHV-2 is one of major agents causing diseases of horses common worldwide. A possible role of EHV-2 in reactivating latent equid herpesvirus type-1 has been suggested, because reactivation of latent EHV-1 was always accompanied by EHV-2 replication. Variety techniques, including cell culture, PCR and its modifications, have been used to diagnose EHV-2 infections. The aim of this study was to develop, optimize and determine specificity of real-time PCR (qPCR) for EHV-2 DNA detection using HybProbesR chemistry and to evaluate clinical samples with this method. The analytical sensitivity of assay was tested using serial dilutions of viral DNA in range between 70 and 7x105 copies/ml. The limit of detection (LOD) was calculated using probit analysis and was determined as 56 copies/ml. In further studies 20 different clinical samples were tested for the presence of EHV-2. Described in-house qPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high level of sensitivity, accuracy and rapidity provided by the LightCycler 2.0 instrument are favorable for the use of this system in the detection of EHV-2 DNA in veterinary virology.
Źródło:: Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Evaluation of methods for Erwinia amylovora detection
Autorzy:: Kaluzna, M.
Pulawska, J.
Mikicinski, A.
Powiązania:: https://bibliotekanauki.pl/articles/1986.pdf
Data publikacji:: 2013
Wydawca:: Instytut Ogrodnictwa
Tematy:: Erwinia amylovora
fire blight
real-time polymerase chain reaction
LAMP technique
DNA sequence
detection method
disease control
Źródło:: Journal of Horticultural Research; 2013, 21, 2
2300-5009
Pojawia się w:: Journal of Horticultural Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Advanced methods of bacteriological identification in a clinical microbiology laboratory
Autorzy:: Zukowska, M.E.
Powiązania:: https://bibliotekanauki.pl/articles/2098275.pdf
Data publikacji:: 2020
Wydawca:: Instytut Medycyny Wsi
Tematy:: clinical microbiology
molecular method
modern method
multiplex polymerase chain reaction
real-time polymerase chain reaction
next generation sequencing
MALDI-TOF mass spectrometry
Opis:: Introduction and objective. Conventional, culture-based methods of bacterial identification and drug-susceptibility testing are considered the gold standard in medical microbiology. In recent years, classical microbiological methods have been supplemented with modern analytical and molecular methods. The aim of the review was to discusses the methods which have been permanently adapted to bacteriological microbiological diagnostics. Abbreviated description of the state of knowledge. Currently, PCR, as well as other nucleic acid amplification tests and sequencing techniques, are part of the standard repertoire of microbiological diagnostics. With regard to the quality and speed of pathogen identification, the introduction of mass spectrometry techniques into routine microbiological diagnostics work-up has been revolutionary. Within a short time in many laboratories, Matrix-Assisted Laser Desorption/Ionisation – Time of Flight Mass Spectrometry (MALDI TOF MS) systems have almost completely replaced conventional biochemical pathogen identification. Conclusions. Microbiological diagnostics is an indispensable element of a targeted therapy. The techniques used in the laboratory depend primarily on the laboratory’s apparatus, the costs of the analysis, as well as the sensitivity and specificity of a method. However, regardless of the culture-based methods universality, advanced techniques have permanently established themselves in diagnostics. Confident information about the detected organism and treatment possibilities in a combination with the clinical context are conducive to successful therapy. Although modern methods still require validation and close collaboration between clinicians, microbiologists and bioinformaticians, these methods, once deemed to be the future, have already arrived.
Źródło:: Journal of Pre-Clinical and Clinical Research; 2021, 15, 2; 68-72
1898-2395
Pojawia się w:: Journal of Pre-Clinical and Clinical Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Reduced expression of AURKA in peripheral blood of breast cancer patients
Autorzy:: Goh, L.P.W.
See, E.U.H.
Chua, K.H.
Lee, P.-C.
Powiązania:: https://bibliotekanauki.pl/articles/81279.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: breast cancer
patient
Aurora kinase
gene expression
peripheral blood
cell cycle
quantitative real-time polymerase chain reaction
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 1
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Dissecting the action of H2O2 after its metabolic formation in chloroplasts
Autorzy:: Jaspert, N.
Balazadeh, S.
Arif, M.
Muller-Rober, B.
Maurino, V.
Powiązania:: https://bibliotekanauki.pl/articles/80107.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
reactive oxygen species
toxic cellular metabolite
signalling molecule
Arabidopsis thaliana
glycolate
chloroplast
real-time polymerase chain reaction
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Molecular cloning and preliminary expression analysis of complete cDNA homologue LOX2 gene in Lupinus luteus
Autorzy:: Wilmowicz, E.
Kucko, A.
Frankowski, K.
Kesy, J.
Marciniak, K.
Glazinska, P.
Banach, M.
Wojciechowski, W.
Kopcewicz, J.
Tretyn, A.
Powiązania:: https://bibliotekanauki.pl/articles/81172.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
molecular cloning
expression analysis
plant hormone
cDNA
LOX2 gene
Lupinus luteus
amino acid sequence
lipoxygenase
real-time polymerase chain reaction
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Regulation of cytochrome b-561 protein family in maize (Zea mays L.) roots by iron and cadmium stress
Autorzy:: Ramanathan, K.
Luthje, S.
Powiązania:: https://bibliotekanauki.pl/articles/79976.pdf
Data publikacji:: 2013
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: maize
Zea mays
root
abiotic stress
cytochrome b561
protein level
real-time polymerase chain reaction
maize root
cadmium stress
iron stress
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Distribution of trichothecene and zearalenone producing Fusarium species in grain of different cereal species and cultivars grown under organic farming conditions in Lithuania
Autorzy:: Suproniene, S
Justesen, A.F.
Nicolaisen, M.
Mankeviciene, A.
Dabkevicius, Z.
Semaskiene, R.
Leistrumaite, A.
Powiązania:: https://bibliotekanauki.pl/articles/49864.pdf
Data publikacji:: 2010
Wydawca:: Instytut Medycyny Wsi
Tematy:: Lithuania
organic farming
farming condition
plant cultivar
cereal
real-time polymerase chain reaction
Fusarium
Fusarium langsethiae
trichothecene
zearalenone
distribution
human health
animal health
Źródło:: Annals of Agricultural and Environmental Medicine; 2010, 17, 1; 79-86
1232-1966
Pojawia się w:: Annals of Agricultural and Environmental Medicine
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Sensitive and specific detection of Xanthomonas hortorum pv. pelargonii in geranium by real-time PCR
Autorzy:: Farahani, A.S.
Taghavi, S.M.
Powiązania:: https://bibliotekanauki.pl/articles/66354.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: detection
bacterial blight
Xanthomonas hortorum pv.pelargonii geranium
real-time polymerase chain reaction
pathogen
Botrytis cinerea
Puccinia pelargonii-zonalis
Pythium
Rhodococcus fascians
Ralstonia solanacearum
Opis:: Xanthomonas hortorum pv. pelargonii is the causal agent of bacterial blight of geranium. A specific and rapid real-time PCR assay for detecting the bacterium in plant was developed in this study. We compared sensitivity of conventional and real-time PCR for detection of the pathogen in inoculated plants. For application to disease management programs, PCR amplification must be able to detect latent infections of asymptomatic geraniums. Our results displayed that conventional PCR lacks sufficient sensitivity to be used as diagnostic tools for detection of X. hortorum pv. pelargonii in latent infections. On the other hand, real-time PCR is suitable method for detection of latent infection of the bacterium in planting materials and can be considered in management programs. The ability for accurate and reliable detection of X. hortorum pv. pelargonii in asymptomatic tissue is a significant step in setting up “pathogen free” certification programs for bacterial blight of geranium.
Źródło:: Journal of Plant Protection Research; 2016, 56, 3
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 15.

Tytuł:: Overexpression of stress-related genes in Cuscuta campestris in response to host defense reactions
Autorzy:: Rezaei, H.
Alamisaeed, K.
Moslemkhani, C.
Powiązania:: https://bibliotekanauki.pl/articles/81031.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Cuscuta campestris
parasitic plant
crop yield
haustorium
RNA extraction
cDNA synthesis
real-time reverse transcription polymerase chain reaction
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2017, 98, 2
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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