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Tytuł:
Zastosowanie techniki PCR w badaniach bakteriologicznych
Applications of PCR in microbiology
Autorzy:
Adaszek, L.
Dziegiel, B.
Mazurek, L.
Winiarczyk, S.
Powiązania:
https://bibliotekanauki.pl/articles/859637.pdf
Data publikacji:
2018
Wydawca:
Krajowa Izba Lekarsko-Weterynaryjna
Tematy:
bakteriologia
diagnostyka bakteriologiczna
metody badan
lancuchowa reakcja polimerazy
16S rRNA
diagnostyka molekularna
Źródło:
Życie Weterynaryjne; 2018, 93, 04
0137-6810
Pojawia się w:
Życie Weterynaryjne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie sekwencji genu rRNA w molekularnej diagnostyce parazytologicznej
Molecular diagnostic of parasites using rRNA gene sequence
Autorzy:
Dlugosz, E
Wisniewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/841440.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
geny kodujace
diagnostyka parazytologiczna
diagnostyka weterynaryjna
wykorzystanie
parazytologia
konferencje
sekwencja genu
gen rRNA
Warszawa konferencja
Opis:
Ribosomal RNA (rRNA) is a component of the ribosomes. Eukaryotic ribosomes contain four different rRNA molecules: 18S, 5,8S, 28S and 5S rRNA. rRNA is the most conserved (least variable) gene in all cells. For this reason, genes that encode the rRNA (rDNA) are sequenced to identify an organism's taxonomic group, calculate related groups, and estimate rates of species divergence. Especially the internal transcribed spacers (ITS) are very useful for molecular diagnostic of parasite. They are noncoding regions of DNA sequence that separate genes coding for the 28S, 5.8S, and 18S ribosomal RNAs. These ribosomal RNA (rRNA) genes are highly conserved across taxa while the spacers between them may be species-specific. In this paper authors describe practical using of rRNA gene to parasite diagnostic.
Źródło:
Annals of Parasitology; 2006, 52, 4; 263-269
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie sekwencji genu rRNA w molekularnej diagnostyce parazytologicznej
Molecular diagnostic of parasites using rRNA gene sequence
Autorzy:
Długosz, E.
Wiśniewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/2144424.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
geny kodujace
diagnostyka parazytologiczna
diagnostyka weterynaryjna
wykorzystanie
parazytologia
konferencje
sekwencja genu
gen rRNA
Warszawa konferencja
Opis:
Ribosomal RNA (rRNA) is a component of the ribosomes. Eukaryotic ribosomes contain four different rRNA molecules: 18S, 5,8S, 28S and 5S rRNA. rRNA is the most conserved (least variable) gene in all cells. For this reason, genes that encode the rRNA (rDNA) are sequenced to identify an organism's taxonomic group, calculate related groups, and estimate rates of species divergence. Especially the internal transcribed spacers (ITS) are very useful for molecular diagnostic of parasite. They are noncoding regions of DNA sequence that separate genes coding for the 28S, 5.8S, and 18S ribosomal RNAs. These ribosomal RNA (rRNA) genes are highly conserved across taxa while the spacers between them may be species-specific. In this paper authors describe practical using of rRNA gene to parasite diagnostic.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 4; 263-269
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Use of molecular and conventional techniques to identify and analyze genetic variability of Rhizoctonia spp. isolates
Zastosowanie metod molekularnych i konwencjonalnych do identyfikacji i analizy zroznicowania genetycznego izolatow Rhizoctonia
Autorzy:
Irzykowska, L
Zoltanska, E
Bocianowski, J
Powiązania:
https://bibliotekanauki.pl/articles/28502.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
pathogenicity
internal transcribed spacer region
rRNA gene
random amplified polymorphic DNA
genetic variability
Rhizoctonia
isolate
Rhizoctonia cerealis
Rhizoctonia solani
molecular technique
conventional technique
stem infection
leaf infection
plant disease
Opis:
In this study the pathogenicity of Rhizoctonia spp. isolates towards wheat seedlings in laboratory and greenhouse conditions was evaluated. In both experiments seven features were examined: plant height, roots weight, the percentage of infected stems and leaf sheaths and also the degree of stem and leaf sheaths infection. Isolates R1, R29, R39 and R59 were the most pathogenic. Percentage of infected stems ranged from 25.3 to 82.5 and roots from 35 to 82.3. The amplification of internal transcribed spacer regions (ITS1 and ITS2) between 18S, 5.8S and 28S rRNA genes and sequence analysis of these regions have been shown to be sufficiently variable to resolve two Rhizoctonia species. Random amplified polymorphic DNA (RAPD) was used to assess genetic variability among isolates. The suitability of RAPD method for isolates differentiation at intraspecific level was shown. Using seven arbitrary primers in polymerase chain reaction (PCR) thirty-three RAPD markers were generated. Clustering analysis from RAPD data resolved two groups of R. cerealis isolates at the 36% similarity level. Moreover, significant associations between molecular markers and pathogenicity of R. cerealis isolates were found.
W prezentowanych badaniach oceniano patogeniczność izolatów Rhizoctonia spp. w stosunku do siewek pszenicy w warunkach laboratoryjnych i szklarniowych. W obu doświadczeniach badano 7 cech: wysokość roślin, masę korzeni, procent porażonych łodyg i pochew liściowych oraz stopień porażenia łodygi i pochwy liściowej. Najbardziej patogeniczne okazały się izolaty R1, R29, R39 i R59. Procent porażonych łodyg mieścił się w zakresie od 25.3 do 82.5, a procent porażonych korzeni od 35 do 82.3. Wykazano, że amplifikacja wewnętrznych transkrybowanych sekwencji rozdzielających (ITS1 i ITS2) geny kodujące 18S, 5.8S i 28S rRNAoraz analiza sekwencyjna tych regionów wystarczyły do rozróżnienia dwóch gatunków rodzaju Rhizoctonia. Losowo amplifikowany polimorficzny DNA (RAPD) wykorzystano do oceny zróżnicowania genetycznego między izolatami. Potwierdzono użyteczność metody RAPD w różnicowaniu izolatów na poziomie wewnątrzgatunkowym. Po zastosowaniu siedmiu losowych starterów w łańcuchowej reakcji polimerazy (PCR) uzyskano 33 markery RAPD. W wyniku analizy klasterów danych uzyskanych w reakcjach RAPD wyodrębniono dwie grupy izolatów R. cerealis na poziomie podobieństwa równym 36%. Ponadto, znaleziono istotne związki pomiędzy markerami molekularnymi a patogenicznością izolatów R. cerealis.
Źródło:
Acta Agrobotanica; 2005, 58, 2; 19-32
0065-0951
2300-357X
Pojawia się w:
Acta Agrobotanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The ribosome filter hypothesis and specialized ribosomes
Autorzy:
Filipek, Kamil
Deryło, Kamil
Michalec-Wawiórka, Barbara
Powiązania:
https://bibliotekanauki.pl/articles/1178213.pdf
Data publikacji:
2018
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
filter hypothesis
rRNA
ribosomal proteins
ribosome
specialized ribosomes
Opis:
The ribosome is a macromolecular complex of proteins and RNA, which plays a key role in every living organism, being a heart of the process of translation. Recent findings have shown that it can be also regarded as a regulatory element that adjusts cellular proteome to highly variable environmental conditions. The ribosome is believed to possess the ability to “filter” populations of mRNAs for choosing their appropriate set to meet current demands of the cell. The filter mechanism is based on a specific interaction between mRNA and rRNA or mRNA and ribosomal proteins. The ribosome “filtering activity” is reflected by the ribosomal particles heterogeneity, which originates mainly from variations or modifications within particular components of translational apparatus. Alternations of ribosomal proteins or/and rRNA generate a specific class of ribosomes called specialized ribosomes, which having unique composition can display selectivity toward particular mRNAs representing an additional step of gene expression regulation at the translational level. This work describes a basis of ribosome filter hypothesis illustrated by interesting examples from different domains of life.
Źródło:
World Scientific News; 2018, 93; 19-29
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The KRR1 gene encodes a protein required for 18S rRNA synthesis and 40S ribosomal subunit assembly in Saccharomyces cerevisiae.
Autorzy:
Gromadka, Robert
Rytka, Joanna
Powiązania:
https://bibliotekanauki.pl/articles/1044220.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
ribosome
rRNA processing
yeast
Opis:
The newly discovered Saccharomyces cerevisiae gene KRR1 (YCL059c) encodes a protein essential for cell viability. Krr1p contains a motif of clustered basic amino acids highly conserved in the evolutionarly distant species from yeast to human. We demonstrate that Krr1p is localized in the nucleolus. The KRR1 gene is highly expressed in dividing cells and its expression ceases almost completely when cells enter the stationary phase. In vivo depletion of Krr1p leads to drastic reduction of 40S ribosomal subunits due to defective 18S rRNA synthesis. We propose that Krr1p is required for proper processing of pre-rRNA and the assembly of preribosomal 40S subunits.
Źródło:
Acta Biochimica Polonica; 2000, 47, 4; 993-1005
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effects of season and processing technology on the abundance of antibiotic resistance genes in air samples from municipal wastewater treatment and waste management plants
Autorzy:
Osińska, Adriana
Jachimowicz, Piotr
Niestępski, Sebastian
Harnisz, Monika
Korzeniewska, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/2032941.pdf
Data publikacji:
2021
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
municipal wastewater
antibiotic resistance
wastewater treatment plant
rRNA
ścieki komunalne
odporność na antybiotyki
oczyszczalnia ścieków
Opis:
This study aimed to perform a qualitative and a quantitative assessment of the prevalence of genes encoding resistance to beta-lactam, tetracycline, and chloramphenicol antibiotics in samples of DNA isolated from air in a municipal wastewater treatment plant (WWTP) and a municipal waste management plant (WMP). Air samples were collected in the mechanical (MP) and biological (BP) processing units of WWTP and WMP in winter and spring. The samples of air were collected by impingement into PBS solution and subsequently, DNA was isolated. The prevalence of the 16S rRNA gene and ARGs was determined by PCR, and the most abundant ARGs were quantified by qPCR. The highest diversity of the analyzed ARGs was noted in air samples collected in the mechanical processing units of the WWTP (winter) and the WMP (spring). The copy of ARGs varied between treatment units and seasons. ARGs were most abundant in air samples collected in spring in the MP units of both the WWTP and the WMP. The study demonstrated that ARGs are ubiquitous in the air in both WWTPs and WMPs. The presence of ARGs in the air can exert a negative impact on the health of plant employees.
Źródło:
Environment Protection Engineering; 2021, 47, 1; 101-114
0324-8828
Pojawia się w:
Environment Protection Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Application of PCR Reaction for Identification of MHB Bacteria Species
Zastosowanie reakcji PCR do identyfikacji gatunkowej bakterii MHB
Autorzy:
Ząbkiewicz, A.
Myga-Nowak, M.
Bandurska, K.
Paczyńska, J.
Szybecka, A.
Krupa, P.
Powiązania:
https://bibliotekanauki.pl/articles/205412.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mycorrhiza
helper bacteria
MHB
16S rRNA gene
mikoryza
bakterie wspomagające
gen 16sRNA
Opis:
This study characterizes mycorrhiza helper bacteria (MHB) from selected unpolluted locations as well as subjected to industrial emissions. To determine the species of bacteria isolated from the roots of ectomycorrhizal pine and birch, a method based on the sequence analysis of a 16S rRNA gene was used. The isolated bacteria were initially characterized by available biochemical methods and phenotypic observation. On the selected bacteria representatives isolation of DNA was performed, on which the PCR reaction was carried out. In this way amplified samples were automatically sequenced and the obtained results were compared to public databases. Among the isolated bacteria Pseudomonas fluorescens SBW25 and Burkholderia xenovorans LB400 species were dominant.
W pracy scharakteryzowano bakterie wspomagające mikoryzę pochodzące z wybranych terenów ekologicznie czystych oraz z terenów poddanych emisji przemysłowej. Przedstawiono wykorzystanie metody opartej na analizie sekwencji genu 16S rRNA do określenia przynależności gatunkowej bakterii wyizolowanych z korzeni ektomikoryzowych sosny i brzozy. Wyizolowane bakterie zostały wstępnie scharakteryzowane przy pomocy dostępnych metod biochemicznych i obserwacji fenotypowej. Dla wybranych przedstawicieli dokonano izolacji DNA, względem którego przeprowadzono reakcję PCR. Powielone w ten sposób próbki automatycznie zsekwencjonowano, a uzyskane sekwencje porównywano w ogólnodostępnych bazach danych. Wśród wyizolowanych bakterii dominowały gatunki Pseudomonas fluorescens SBW25 oraz Burkholderia xenovorans LB400.
Źródło:
Archives of Environmental Protection; 2014, 40, 2; 115-122
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Taxonomic Descriptions of Two Marine Ciliates, Euplotes dammamensis n. sp. and Euplotes balteatus (Dujardin, 1841) Kahl, 1932 (Ciliophora, Spirotrichea, Euplotida), Collected from the Arabian Gulf, Saudi Arabia
Autorzy:
Chen, Xumiao
Zhao, Yan
Al-Farraj, Saleh A.
AL-QURAISHY, Saleh
El-Serehy, Hamed A.
Shao, Chen
Al-Rasheid, Khaled A. S.
Powiązania:
https://bibliotekanauki.pl/articles/763475.pdf
Data publikacji:
2013
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
Marine ciliate, morphogenesis, morphology, new species, SSU-rRNA, taxonomy
Opis:
The morphology, morphogenesis and infraciliature of two marine euplotid ciliates, Euplotes dammamensis n. sp. and Euplotes balteatus (Dujardin, 1841) Kahl, 1932, isolated from a sandy beach of the Arabian Gulf, Saudi Arabia, were investigated using observations in vivo and protargol-impregnation methods. Euplotes dammamensis n. sp. is characterized by a combination of features including its huge body size (100–170 × 80–120 μm), 10 conspicuous dorsal ridges, 10 normal-sized frontoventral and two marginal cirri, and 11 dorsal kineties. Euplotes balteatus is mainly characterized by 10 frontoventral, two caudal, and two left marginal cirri, 7–10 dorsal kineties and 5–7 prominent dorsal ridges as well as double-eurystomus silverline system. The small subunit rRNA (SSU-rRNA) gene sequences were determined for both species and phylogenetic analyses based on these data indicated that E. dammamensis is most closely related to E. parabalteatus Jiang et al., 2010, and E. balteatus clusters with E. plicatum Valbonesi et al., 1997, E. orientalis Jiang et al., 2010, and E. bisulcatus Kahl, 1932.
Źródło:
Acta Protozoologica; 2013, 52, 2
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structure and functions of 5S rRNA.
Autorzy:
Barciszewska, Mirosława
Szymański, Maciej
Erdmann, Volker
Barciszewski, Jan
Powiązania:
https://bibliotekanauki.pl/articles/1044186.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
50S subunit structure
ribosomal 5S RNA
5S rRNA
crystal structure
Opis:
The ribosome is a macromolecular assembly that is responsible for protein biosynthesis in all organisms. It is composed of two-subunit, ribonucleoprotein particles that translate the genetic material into an encoded polypeptides. The small subunit is the site of codon-anticodon interaction between the messenger RNA (mRNA) and transfer RNA (tRNA) substrates, and the large subunit catalyses peptide bond formation. The peptidyltransferase activity is fulfilled by 23S rRNA, which means that ribosome is a ribozyme. 5S rRNA is a conserved component of the large ribosomal subunit that is thought to enhance protein synthesis by stabilizing ribosome structure. This paper shortly summarises new results obtained on the structure and function of 5S rRNA.
Źródło:
Acta Biochimica Polonica; 2001, 48, 1; 191-198
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Some corrosive bacteria isolated from the technogenic soil ecosystem in Chernihiv city (Ukraine)
Autorzy:
Tkachuk, Nataliia
Zelena, Liubov
Powiązania:
https://bibliotekanauki.pl/articles/2026000.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
16S rRNA gene
microbial induced corrosion
phenotypic characteristics
Opis:
The soil microbiome is exposed to technogenic influence during the operation of metal structures. There are quantitative and qualitative changes in the microbiota of the technogenic ecosystem. During the study of the technogenic soil ecosystem (ferrosphere), samples of which were taken in the field (Chernihiv, Ukraine: 51°29’58”N, 31°16’09”E), the presence of corrosively active microbial cenosis was established: sulfate-reducing, denitrifying, iron-reducing (using acetate as the only electron donor, and Fe (III) as the only electron acceptor) and ammonifying bacteria. The predominant representatives of corrosively active groups of bacteria were isolated. They were identified as Bacillus simplex, Streptomyces gardneri, Streptomyces canus (ammonifying bacteria), Fictibacillus sp. (ammonifying bacteria with iron-reducing ability), Anaerotignum (Clostridium) propionicum (organic acid-producing bacteria), Desulfovibrio oryzae (sulfate-reducing bacteria) based on some microbiological, physiological and biochemical, genetic features. Strains of heterotrophic and hemolitotrophic bacteria (individual representatives and their associations) isolated from the technogenic ecosystem can be used in both industrial and technological spheres. The interaction of isolated bacteria in the process of microbial induced corrosion is a prospect for further research.
Źródło:
Studia Quaternaria; 2021, 38, 2; 101-108
1641-5558
2300-0384
Pojawia się w:
Studia Quaternaria
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Soil microbiomes of reclaimed and abandoned mines of the Yamal region
Autorzy:
Pershina, Elizaveta
Ivanova, Ekaterina
Kimeklis, Anastasia
Zverev, Alexey
Kichko, Arina
Aksenova, Tatiana
Andronov, Evgeny
Abakumov, Evgeny
Powiązania:
https://bibliotekanauki.pl/articles/2041836.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Arctic
Yamal Peninsula
microbiome
soil
high-throughput sequencing
16S rRNA
qPCR
mining
reclamation
Źródło:
Polish Polar Research; 2020, 41, 1; 95-114
0138-0338
2081-8262
Pojawia się w:
Polish Polar Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reconstruction of Evolutionary History of Pleurostomatid Ciliates (Ciliophora, Litostomatea, Haptoria): Interplay of Morphology and Molecules
Autorzy:
VĎAČNÝ, Peter
SHIN, Mann Kyoon
KIM, Ji Hye
JANG, Seok Won
SHAZIB, Shahed Uddin Ahmed
RAJTER, Ľubomír
Powiązania:
https://bibliotekanauki.pl/articles/763636.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
Tematy:
18S rRNA gene, Acineria, Epiphyllidae fam. nov., Kentrophyllum, Korea
Opis:
Pleurostomatids are raptorial ciliates that form a very distinct group within the Haptoria. Traditionally, the order Pleurostomatida was divided into two families: the Amphileptidae with two perioral kineties and a suture formed by the right side ciliary rows, and the Litonotidae with three perioral kineties and without suture. However, molecular phylogenies depicted the “traditional” Amphileptidae as a paraphyletic assemblage nesting also the Litonotidae. To overcome this problem we have analyzed genealogy of pleurostomatids using morphological data and 18S rRNA gene sequences, including newly sequenced genera Acineria and Kentrophyllum. Specifically, we have combined a morphological and molecular approach and have used also some other phylogenetic tools such as phylogenetic networks, split spectrum analysis, quartet mapping as well as the likelihood method of tracing history of morphological characters. These analyses show that: (1) there are not two but three distinct pleurostomatid lineages – Epiphyllidae fam. nov., Amphileptidae and Litonotidae; (2) epiphyllids (Epiphyllum + Kentrophyllum) represent a basal pleurostomatid group which is defined by two perioral kineties, by the presence of a suture on both the right and the left side of the body, by the loss of the oral bulge extrusomes, and by the extrusome fringe extending all around the body except for the oral region; (3) the families Amphileptidae and Litonotidae are monophyletic each, and represent sister groups; (4) Acineria belongs to the Litonotidae, as already indicated by morphological data; (5) Loxophyllum is a monophyletic and crown genus of the Litonotidae; and (6) Litonotus is paraphyletic, which could be very likely caused by a rapid radiation event that did not allow primary nucleotide homologies to be fixed.
Źródło:
Acta Protozoologica; 2015, 54, 1
1689-0027
Pojawia się w:
Acta Protozoologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks determined by polymerase chain reaction with two pairs of primers detecting 16S rRNA and ankA genes
Autorzy:
Chmielewska-Badora, J
Zwolinski, J.
Cisak, E.
Wojcik-Fatla, A.
Buczek, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51023.pdf
Data publikacji:
2007
Wydawca:
Instytut Medycyny Wsi
Tematy:
Lublin region
Polska
pathogen
Ixodes ricinus
Eastern Poland
tick
determination
animal pathogen
human pathogen
ankA gene
16S rRNA gene
granulocytic anaplasmosis
bacteria
Anaplasma phagocytophilum
polymerase chain reaction
Opis:
A total of 684 Ixodes ricinus ticks (321 nymphs, 184 males, and 179 females) were collected by fl agging lower vegetation in 6 forest districts located on the territory of Lublin province (eastern Poland). Ticks were examined by polymerase chain reaction (PCR) method for the presence of Anaplasma phagocytophilum DNA with two pairs of primers: EHR521/EHR747 for detecting 16S rRNA gene, and LA6/LA1 for detecting ankA gene. To study the relationship between infection in ticks and people occupationally exposed to tick bite, blood serum samples of 261 forestry workers employed in the same forest districts were examined by immunofl uorescence method for the presence of specifi c antibodies against A. phagocytophilum. A total of 70 ticks out of 684 examined (10.2%) showed the presence of A. phagocytophilum 16S rRNA gene. The prevalence of infection was signifi cantly dependent on tick’s stage (χ2=49.2, p<0.00001) and geographical locality (χ2=34.4, p<0.00001). The percentage of I. ricinus females infected with A. phagocytophilum (24.6%) was signifi cantly greater compared to males (6.5%) and nymphs (4.4%) (p<0.00001). Only 19 ticks out of 684 examined (2.8%) showed the presence of A. phagocytophilum ankA gene, signifi cantly less compared to 16S rRNA gene (p<0.00001). The prevalence of infection demonstrated by the presence of ankA gene was also signifi cantly dependent on tick’s stage (χ2=23.6, p<0.00001) but not on locality (χ2=9.8, p=0.082). A signifi cant correlation was found between the presence of A. phagocytophilum 16S rRNA gene in I. ricinus female ticks from the particular forest districts and the serologic response to A. phagocytophilum of forestry workers employed in the same districts (p<0.05). No signifi cant correlation was found between the presence of A. phagocytophilum ankA gene in I. ricinus ticks and serologic response of exposed workers. In conclusion, detection of A. phagocytophilum infection in ticks by PCR with the use of EHR521/EHR747 primers detecting 16S rRNA gene is signifi cantly more sensitive compared to LA6/LA1 primers detecting ankA gene.
Źródło:
Annals of Agricultural and Environmental Medicine; 2007, 14, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polyadenylation and decay of 26S rRNA as part of Nicotiana tabacum response to cadmium
Autorzy:
Lewandowska, Małgorzata
Borcz, Barbara
Kamińska, Jolanta
Wawrzyński, Adam
Sirko, Agnieszka
Powiązania:
https://bibliotekanauki.pl/articles/1040862.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
polyadenylation of rRNA
RNA decay
programmed cell death
cadmium
tobacco
Opis:
In contrast to mRNAs, ribosomal RNAs are generally not considered to be polyadenylated. Only a few recent reports describe non-abundant polyadenylated rRNA-related transcripts that have been detected and characterized in yeast and in human cells. Here we depict the phenomenon of 26S rRNA polyadenylation and degradation that was observed in shoots of Nicotiana tabaccum plants grown in the presence of cadmium. Fragments corresponding to 26S rRNA were identified using suppression subtractive hybridization during screening for genes induced in tobacco plants upon a three-week exposure to 15 µM cadmium chloride. Extracts prepared from the above-ground tissues of cadmium-treated tobacco plants were supposed to contain exclusively polyadenylated mRNAs. Surprisingly, numerous polyadenylated fragments matching parts of 26S rRNA were identified and their presence was confirmed by Northern blot and cDNA amplification techniques. To our knowledge this is the first report on rRNA polyadenylation in plants.
Źródło:
Acta Biochimica Polonica; 2007, 54, 4; 747-755
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 48

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