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Wyświetlanie 1-15 z 49
Tytuł:
New proteases of the chloroplast envelope – what do they do there?
Autorzy:
Adam, Z.
Powiązania:
https://bibliotekanauki.pl/articles/80681.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
protease
chloroplast
thylakoid
proteomics
allene oxide synthase
jasmonic acid
serine protease
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteolytic activity in the midgut of the crimson speckled moth Utethesia pulchella L. (lepidoptera: arctiidae)
Autorzy:
Ajamhassani, M.
Zibaee, A.
Sendi, J.J.
Askary, H.
Farrar, N.
Powiązania:
https://bibliotekanauki.pl/articles/961656.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
utethesia pulchalle
midgut
protease
inhibitor
Opis:
Samples were prepared from the midgut of 4th instar larvae of the crimson speckled moth Utethesia pulchella L. to find proteolytic activity and properties. Result revealed the presence of high proteolytic activity in the midgut when taking into account specific proteinases including trypsin-like, chymotrypsin-like, elastase and two exopeptidase (aminopeptidase and carboxipeptidase). The optimal pH of general protease was 8 and 7 when using azocasein and hemoglobin as general substrates, respectively. The optimal temperature of the total proteolytic activity in the midgut of U. pulchella was 25°C and 30°C when using azocasein and hemoglobin, respectively. Proteolytic activity was inhibited significantly by soybean trypsin inhibitor (SBTI), phenylmethylsulfonyl fluoride (PMSF), trypsin inhibitor (TLCK), chymotrypsin inhibitor (TPCK) and Phenanthroline. These results provide evidences for the presence of serine proteinases as the major proteases in the midgut of U. pulchella; a key rangeland pest in warm climates. The interaction between digestive proteases and protease inhibitors have potentially important consequences for pest management programs.
Źródło:
Journal of Plant Protection Research; 2012, 52, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Three Pseudomonas aeruginosa strains with different protease profiles
Autorzy:
Andrejko, Mariola
Zdybicka-Barabas, Agnieszka
Janczarek, Monika
Cytryńska, Małgorzata
Powiązania:
https://bibliotekanauki.pl/articles/1039614.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
aprA
alkaline protease
extracellular proteases
elastase B
virulence
lasB
Pseudomonas aeruginosa
Opis:
The proteolytic activity of three Pseudomonas aeruginosa strains, ATCC 27853 - a reference strain, and two clinical isolates was tested. The activity was examined after culturing the bacteria in two different growth media: the minimal M9 medium and rich Luria-Bertani broth (LB). Based on zymograms and protease activity specific assays, it was concluded that the reference strain produced three proteolytic enzymes in the LB medium: protease IV, elastase B and elastase A, while alkaline protease was only produced in the M9 medium. The clinical isolates of P. aeruginosa produced elastase B and alkaline protease when grown in the LB medium and the minimal M9 medium, respectively. PCR analysis confirmed the presence of both the lasB gene encoding elastase B and aprA coding for alkaline protease in the genomes of the three P. aeruginosa strains analyzed. The expression of these genes coding for two important P. aeruginosa virulence factors was dependent on the growth conditions in all the strains studied. The contribution of the extracellular proteinases to the virulence of P. aeruginosa strains used in this study was investigated using an insect model, the greater wax moth Galleria mellonella.
Źródło:
Acta Biochimica Polonica; 2013, 60, 1; 83-90
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Downregulation of chloroplast protease AtDeg5 leads to changes in chronological progression of ontogenetic stages, leaf morphology and chloroplast ultrastructure in Arabidopsis
Autorzy:
Baranek, M.
Wyka, T.P.
Jackowski, G.
Powiązania:
https://bibliotekanauki.pl/articles/57852.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
chloroplast protease
AtDeg5 protease
chronological progression
ontogenetic stage
leaf morphology
chloroplast ultrastructure
Arabidopsis
starch grain
Opis:
The chloroplast protein AtDeg5 is a serine-type protease peripherally attached to thylakoid membrane at its lumenal side. Since reliable data regarding the role of AtDeg5 in controlling the course of growth and developmental processes are extremely limited, two independent T-DNA insertional lines with different extent of AtDeg5 reduction were prepared and ontogenesis stage-based analysis performed. Both mutant lines displayed a compensatory overaccumulation of AtDeg8. The repression of AtDeg5 protease altered a range of phenotypic features in at least one of the mutants, with the most prominent being changes in chronological progression of development and growth of individual rosette leaves, flower production and silique ripening as well as in the area of fully expanded leaves and chloroplast ultrastructure. By analyzing the results of parallel-mutant screening we conclude that AtDeg8 overdose may rescue 23% of AtDeg5 deficiency with regard to some AtDeg5-controlled traits; alternatively AtDeg5 may have catalytic sites in excess so that these traits might remain unaltered when AtDeg5 pool is reduced by 23%. For some other AtDeg5-dependent traits the absence of excessive amount of AtDeg5 catalytic sites, lack of AtDeg5 dosage effect and inability of AtDeg8 to compensate deficiency or absence of AtDeg5 occurred.
Źródło:
Acta Societatis Botanicorum Poloniae; 2015, 84, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The regulatory role of AtDeg5 chloroplast protease in chronological progression of principal growth stages in Arabidopsis thaliana plants
Autorzy:
Baranek, M.
Lucinski, R.
Jackowski, G.
Powiązania:
https://bibliotekanauki.pl/articles/80598.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
serine-type protease
chymotrypsin
thylakoid membrane
photosystem II
protease
chloroplast
Arabidopsis thaliana
ontogenesis
growth stage
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Binding of Aedes aegypti trypsin modulating oostatic factor [Aea-TMOF] to its receptor stimulates phosphorylation and protease processing of gut-membrane proteins
Autorzy:
Borovsky, D.
Hamdaoui, A.
Powiązania:
https://bibliotekanauki.pl/articles/55079.pdf
Data publikacji:
2008
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
protease processing
Aedes aegypti
electrophoresis
trypsin modulating oostatic factor
phosphorylation
mosquito
insect
larva
gut-membrane protein
fluorography
gut receptor
Opis:
The binding of TMOF to its gut receptor was followed by incubating guts removed from male and female Aedes aegypti. TMOF at physiological concentrations, in the presence of [γ32P]ATP, causes phosphorylation and release of gut-membrane protein (45 kDa) that is further processed by proteolysis. In the presence of protease inhibitors only the 45 kDa protein was released. The phosphorylation and processing of the 45 kDa protein does not happen in the absence of TMOF. Both larvae and adult guts release the protein in the presence of TMOF. Male Ae. aegypti do not synthesize trypsin in their gut and do not release the 45 kDa protein in the presence of TMOF because a TMOF receptor is probably absent. Homogenized guts do not release the 45 kDa protein, indicating that the protease processing or the ecto-protein kinase activity is probably reduced after breaking the tissue. The 45 kDa phosphorylated protein can be dephosphorylated by alkaline phosphatase and protein phosphatase, indicating that the phosphate group is covalently linked to either a serine or a tyrosine moiety. This is the first report that shows that in insects, binding of a peptide hormone activates its receptor by proteolysis.
Źródło:
Pestycydy; 2008, 1-2; 13-25
0208-8703
Pojawia się w:
Pestycydy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mitochondrial protease AtFtsH4 affects the level of oxidatively modified proteins in seeds and mitochondria of A. thaliana
Autorzy:
Czarna, M.
Smakowska, E.
Janska, H.
Powiązania:
https://bibliotekanauki.pl/articles/80803.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
protein carbonylation
oxidative modification
plant
life cycle
AtFtsH4 protease
seed
mitochondrion
Arabidopsis thaliana
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Theoretical studies of binding modes of two covalent inhibitors of cysteine proteases.
Autorzy:
Drabik, Piotr
Politowska, Ewa
Czaplewski, Cezary
Kasprzykowski, Franciszek
Łankiewicz, Leszek
Ciarkowski, Jerzy
Powiązania:
https://bibliotekanauki.pl/articles/1044228.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cysteine proteases
covalent protease inhibitors
constrained simulated annealing
papain
molecular dynamics
Opis:
Physiological and pathological roles of cysteine proteases make them important targets for inhibitor development. Although highly potent inhibitors of this group of enzymes are known, their major drawback is a lack of sufficient specificity. Two cysteine protease covalent inhibitors, viz. (i) Z-RL-deoxo-V-peptide-epoxysuccinyl hybrid, and (ii) Z-RLVG-methyl-, have been developed and modeled in the catalytic pocket of papain, an archetypal thiol protease. A number of configurations have been generated and relaxed for each system using the AMBER force field. The catalytic pockets S3 and S4 appear rather elusive in view of the observed inhibitors' flexibility. This suggest rather limited chances for the development of selective structure-based inhibitors of thiol proteases, designed to exploit differences in the structure of catalytic pockets of various members of this family.
Źródło:
Acta Biochimica Polonica; 2000, 47, 4; 1061-1066
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The use of enzymatic fungal activity in the food industry - review
Autorzy:
Drozłowska, Emilia
Powiązania:
https://bibliotekanauki.pl/articles/1076386.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
amylases
enyzmes
fungi
laccase
protease
Opis:
Enzymes are increasingly used in the food industry, due to the fact that they allow to streamline many processes. They catalyse processes that require energy and are long-lasting. Mushrooms produce hydrolytic enzymes such as proteases, lipases and amylases. These enzymes are used in winemaking, brewing, confectionery and cheese production. Attempts have also been made to use paper industry enzymes such as laccase in the wine industry. The production of enzymes of animal origin is an expensive and complicated process, which is why they were interested in their production from microorganisms. This article attempts to review the current state of knowledge on the use of fungal enzymes in the food industry.
Źródło:
World Scientific News; 2019, 116; 222-229
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New generation of peptide antibiotics
Autorzy:
Dubin, Adam
Mak, Paweł
Dubin, Grzegorz
Rzychon, Małgorzata
Stec-Niemczyk, Justyna
Wladyka, Benedykt
Maziarka, Katarzyna
Chmiel, Dorota
Powiązania:
https://bibliotekanauki.pl/articles/1041366.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
staphostatin
Staphylococcus
virulence factor
hemoglobin
protease inhibitor
hemocidins
antimicrobial agents
antibiotics
Opis:
The increasing antibiotic resistance of pathogenic bacteria calls for the development of alternative antimicrobial strategies. Possible approaches include the development of novel, broad-spectrum antibiotics as well as specific targeting of individual bacterial virulence factors. It is impossible to decide currently which strategy will prove more successful in the future since they both promise different advantages, but also introduce diverse problems. Considering both approaches, our laboratory's research focuses on the evaluation of hemocidins, broad-spectrum antibacterial peptides derived from hemoglobin and myoglobin, and staphostatins, specific inhibitors of staphopains - Staphylococcus aureus secreted proteases that are virulence factors regarded as possible targets for therapy. The article summarizes recent advances in both fields of study and presents perspectives for further development and possible applications.
Źródło:
Acta Biochimica Polonica; 2005, 52, 3; 633-638
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Defense against own arms: staphylococcal cysteine proteases and their inhibitors.
Autorzy:
Dubin, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1043443.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
staphostatin
staphylococcus
ssp
proteinase
protease inhibitor
staphopain
Opis:
Staphylococcus aureus is a human pathogen causing a wide range of diseases. Most staphylococcal infections, unlike those caused by other bacteria are not toxigenic and very little is known about their pathogenesis. It has been proposed that a core of secreted proteins common to many infectious strains is responsible for colonization and infection. Among those proteins several proteases are present and over the years many different functions in the infection process have been attributed to them. However, little direct, in vivo data has been presented. Two cysteine proteases, staphopain A (ScpA) and staphopain B (SspB) are important members of this group of enzymes. Recently, two cysteine protease inhibitors, staphostatin A and staphostatin B (ScpB and SspC, respectively) were described in S. aureus shedding new light on the complexity of the processes involving the two proteases. The scope of this review is to summarize current knowledge on the network of staphylococcal cysteine proteases and their inhibitors in view of their possible role as virulence factors.
Źródło:
Acta Biochimica Polonica; 2003, 50, 3; 715-724
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of Anisakis simplex stage III larvae upon the activity of proteases under in vitro conditions
Autorzy:
Dziekonska-Rynko, J
Rokicki, J.
Jablonowski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/837165.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
protease activity
stage
papain
pepsin
trypsin
larva
Anisakis simplex
in vitro
Opis:
The larvae of Anisakis simplex had the largest influence upon decreasing the activity of porcine pepsin. The activity of that enzyme in tests, where the larvae were present during the entire period of incubation, was lower than in the controls. No similar trends were observed in case of the solutions with bovine and porcine trypsin. The activity of those enzymes in the solutions containing the larvae was higher than in the controls. Only the activity of porcine trypsin after 10 h of incubation was slightly lower in the experimental sample than in the control, however, during the later hours the dynamics of the activity decrease of that enzyme in the controls was higher than in the experimental samples. The recorded activity of papain in the samples containing the larvae was higher than that in the controls during the entire time of the experiment.
Źródło:
Annals of Parasitology; 2002, 48, 2
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of Anisakis simplex stage III larvae upon the activity of proteases under in vitro conditions
Autorzy:
Dziekońska-Rynko, J.
Rokicki, J.
Jabłonowski, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2147699.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
protease activity
stage
papain
pepsin
trypsin
larva
Anisakis simplex
in vitro
Opis:
The larvae of Anisakis simplex had the largest influence upon decreasing the activity of porcine pepsin. The activity of that enzyme in tests, where the larvae were present during the entire period of incubation, was lower than in the controls. No similar trends were observed in case of the solutions with bovine and porcine trypsin. The activity of those enzymes in the solutions containing the larvae was higher than in the controls. Only the activity of porcine trypsin after 10 h of incubation was slightly lower in the experimental sample than in the control, however, during the later hours the dynamics of the activity decrease of that enzyme in the controls was higher than in the experimental samples. The recorded activity of papain in the samples containing the larvae was higher than that in the controls during the entire time of the experiment.
Źródło:
Wiadomości Parazytologiczne; 2002, 48, 2; 217-223
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of selected physico-chemical factors on Baltic herring meat hydrolysis rate and proteolytic enzyme activity
Wpływ niektórych czynników fizykochemicznych na aktywność enzymów proteolitycznych i szybkość hydrolizy białek mięsa śledzia bałtyckiego
Autorzy:
Fik, M.
Sypniewska, L.
Thi, N.P.
Powiązania:
https://bibliotekanauki.pl/articles/1396371.pdf
Data publikacji:
1985
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
Baltic herring
proteolytic enzyme
protein autohydrolysis
papin
cathepsin
B-500 protease
Opis:
The effect of some physico-chemical factors on the rate of Baltic herring meat protein autohydrolysis and hydrolysis due to papain and B-500 protease from Bacillus subtilis is reported.
Badano wpływ niektórych czynników fizykochemicznych (pH, temperatura, czas inkubacji, dodatek NaCl) na aktywność enzymów proteolitycznych i szybkość hydrolizy białek mięsa śledzia bałtyckiego. Stwierdzono maksimum aktywności proteinaz mięśniowych w pH 4,2 a proteinaz rozdrobnionej ryby całej w pH 3,7 i 8,3 z tym, że hydroliza w pH 3,7 była intensywniejsza niż w pH 8,3 (rys. 1). W obu przypadkach największą aktywność endogennych enzymów proteolitycznych wykazano w temp. 50°C (rys. 2). Stosowany w charakterze konserwanta chlorek sodu w stężeniach do 10 g na 100 g rozdrobnionej ryby całej powodował nieznaczne hamowanie aktywności enzymów tego surowca po krótkiej hydrolizie i wpływał na nie stymulująco po długim czasie hydrolizy (rys. 3). Większe stężenia NaCl znacznie zmniejszały szybkość hydrolizy białek. W celu zwiększenia szybkości hydrolizy białek ryby całej zastosowano papainę (rys. 4) i proteazę B-500 (rys. 5), z których ta ostatnia, pomimo większego inaktywującego wpływu chlorku sodu, była aktywniejsza niż papaina. Oba preparaty enzymatyczne charakteryzowały się mniejszą podatnością na inaktywujący wpływ NaCl, aniżeli endogenne enzymy proteolityczne surowca. Do hydrolizy śledzia w warunkach przemysłowych spośród tych dwóch preparatów lepiej nadaje się proteaza B-500.
Źródło:
Acta Alimentaria Polonica; 1985, 11, 3; 305-312
0137-1495
Pojawia się w:
Acta Alimentaria Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Purification and characterization of a novel metalloprotease from fruiting bodies of Oudemansiella radicata
Autorzy:
Geng, Xueran
Te, Rigen
Tian, Guoting
Zhao, Yongchang
Zhao, Liyan
Wang, Hexiang
Ng, Tzi
Powiązania:
https://bibliotekanauki.pl/articles/1038592.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
edible mushroom
Oudemansiella radicata
protease
purification
Opis:
In this study, a 39-kDa metalloprotease was purified from a rare edible mushroom with health-promoting activities, Oudemansiella radicata, using a purification protocol which entailed anion exchange chromatography on DEAE-cellulose and Q-Sepharose columns and gel filtration by fast protein liquid chromatography on a Superdex 75 column. Some peptide sequences were obtained by LC-MS/MS analysis and one of the sequences, DAWIQADVNR, manifested 90% identity to Coprinopsis cinerea metalloprotease. The optimal reaction pH and temperature for Oudemansiella radicata protease were pH 7.0 and 50°C, respectively. The protease was purified 79-fold and demonstrated a specific protease activity of 2.42 U/mg. The Km of the purified protease for the casein substrate was 0.65 mg/mL at pH 7.0 and 50°C. The activity of the protease was inhibited by Cd2+, Hg2+, Cu2+, Pb2+ and Fe3+ ions, but was enhanced by K+, Mn2+ and Fe2+ ions. The marked suppression of the protease activity by EDTA indicates that the protease is a metalloprotease.
Źródło:
Acta Biochimica Polonica; 2017, 64, 3; 477-483
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 49

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