Temat: plant virus - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Plant virus resistance biotechnological approaches : From genes to the CRISPR/Cas gene editing system
Autorzy:: Iksat, Nurgul
Masalimov, Zhaksylyk
Omarov, Rustem
Powiązania:: https://bibliotekanauki.pl/articles/27312654.pdf
Data publikacji:: 2023
Wydawca:: Instytut Technologiczno-Przyrodniczy
Tematy:: CRISPR/Cas9
CRISPR/Cas13
plant disease resistance
plant immunity
plant virus
Opis:: Plant viruses cause crop losses in agronomically and economically important crops, making global food security a challenge. Although traditional plant breeding has been effective in controlling plant viral diseases, it is unlikely to solve the problems associated with the frequent emergence of new and more virulent virus species or strains. As a result, there is an urgent need to develop alternative virus control strategies that can be used to more easily contain viral diseases. A better understanding of plant defence mechanisms will open up new avenues for research into plant-pathogen interactions and the development of broad-spectrum virus resistance. T he scientific literature was evaluated and structured in this review, and the results of the reliability of the methods of analysis used were filtered. As a result, we described the molecular mechanisms by which viruses interact with host plant cells. To develop an effective strategy for the control of plant pathogens with a significant intensity on the agricultural market, clear and standardised recommendations are required. The current review will provide key insights into the molecular underpinnings underlying the coordination of plant disease resistance, such as main classes of resistance genes, RNA interference, and the RNA-mediated adaptive immune system of bacteria and archaea - clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated Cas proteins - CRISPR/Cas. Future issues related to resistance to plant viral diseases will largely depend on integrated research to transfer fundamental knowledge to applied problems, bridging the gap between laboratory and field work.
Źródło:: Journal of Water and Land Development; 2023, 57; 147--158
1429-7426
2083-4535
Pojawia się w:: Journal of Water and Land Development
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Population genetics analysis of Garlic virus A, Garlic virus B, Garlic virus C and Garlic virus X
Autorzy:: Bereda, M.
Paduch-Cichal, E.
Powiązania:: https://bibliotekanauki.pl/articles/12683733.pdf
Data publikacji:: 2019
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: plant cultivation
garlic
Allium
population genetics
genetic analysis
plant virus
garlic virus A
garlic virus B
garlic virus C
garlic virus X
Opis:: Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and Garlic virus X (GarV-X) are members of the genus Allexivirus in the family Alphaflexiviridae. In this study, we collected 10, 30, 10 and 14 isolates of GarV-A, GarV-B, GarV-C and GarV-X, respectively, from different parts of Poland. All sequences of coat protein (CP) and nucleic-acid binding protein (NABP) regions of Allexivirus isolates available in GenBank were also included in this study. The nucleotide and amino acid sequences identities within each population differed substantially depending on the region of the genome and virus species. The results of selection pressure analysis showed that populations of each Allexivirus underwent negative selection, but the extent of the negative selection varied. It was also concluded that the GarV-A and GarV-C populations underwent a decrease in population size or balancing selection, while the GarV-B and GarV-X populations underwent an increase in population size. It was concluded that both populations of GarV-X evolved independently in each respective area, in contrast to populations of GarV-A, GarV-B and GarV-C.
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 3; 99-115
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Influence of a prolong application of mineral oils on bulb yield, quality of cut flowers and spread of viruses in tulip cultivation
Autorzy:: Marcinek, B.
Karczmarz, K.
Szmagara, M.
Durlak, W.
Pogroszewska, E.
Powiązania:: https://bibliotekanauki.pl/articles/11886651.pdf
Data publikacji:: 2018
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: plant cultivation
tulip
Leen van der Mark cultivar
flowering plant
bulbous plant
cut flower
flower quality
mineral oil
virus spreading
field cultivation
field experiment
greenhouse cultivation
greenhouse experiment
Sunspray 850 EC preparation
Opis:: Identification of cultivars is essential both in breeding and to settle cultivar disputes. The purpose of the study has been to examine cultivar identities based on absorption spectra of plant pigments and to confirm a genetic stability with SCoT and RAPD molecular markers in new Polish lines of Chenopodium quinoa Willd. Spectral analysis of pigments extracted from plant inflorescences in quinoa gives an opportunity to confirm the cultivar identity and identification of ‘Faro’ and ‘Titicaca’ cultivars and their new lines. Spectral analysis is an effective method of confirming cultivar identity and it should be used in practice for the identification of cultivars or cultivars lines in Chenopodium quinoa Willd. Analysis of molecular markers indicated by RAPD as well as SCoT technique revealed a high genetic stability of the derivative lines of ‘Faro’ and ‘Titicaca’, while variation was detected in plants representing original cultivars: banding pattern different than predominant was present in three plants of ‘Titicaca’ (genetic distnaces from 7.5% to 55.9%) and in a single plant of ‘Faro’(genetic distance 61.2% as indicated by SCoT technique).
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 1; 115-125
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Management of potato virus Y (PVY–NTN) causing potato tuber necrotic ringspot disease (PTNRD) in potato by prior treatment with a mild PVY strain
Autorzy:: Abdalla, O.A.
Eraky, A.I.
Mohamed, S.A.
Fahmy, F.G.
Powiązania:: https://bibliotekanauki.pl/articles/65943.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Egypt
pest management
potato virus Y
potato tuber
necrotic ringspot disease
potato
plant protection
mild strain
treatment
Opis:: Potato virus Y (PVY) is one of the most destructive viruses infecting potato in Egypt and worldwide. Recent research has shown that a necrotic PVY-NTN strain is infecting potato in Upper Egypt. Chemical control is not effective to control this viral pathogen. An alternative to control PVY infecting potato is using a mild PVY strain to elicit systemic cross protection in potato plants against infection with a severe necrotic strain of PVY. Results of this study showed that a PVY necrotic strain produced a significant lesser number of local lesions on diagnostic plants (Robinia pseudoacacia L.) when these plants were treated first with a mild PVY strain. Data obtained from greenhouse and field experiments indicated that treatment of potato plants (variety Burna) with a mild PVY strain significantly protected potato from infection with a severe necrotic PVY strain, and resulted in a significant increase in tuber yield compared with infected plants without prior treatment with a mild PVY strain. The highest increase in potato tuber yield was obtained when potato plants were inoculated with a mild PVY strain 3 days before challenging with the severe necrotic PVY strain. This study proved that using a mild strain of PVY can significantly protect potato plants from infection with a severe strain of this virus under both greenhouse and field conditions and can present a potential method to reduce losses due to infection of this virus in Assiut governorate and Upper Egypt.
Źródło:: Journal of Plant Protection Research; 2018, 58, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Plant parasitic nematodes in the soil and roots of winter wheat grown in crop rotation and long-term monoculture
Autorzy:: Zaton, K.K.
Skwiercz, A.T.
Adamiak, E.
Szelagowska, P.
Hury, G.
Powiązania:: https://bibliotekanauki.pl/articles/66002.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: plant parasite
parasitic nematode
nematode
pathogenic fungi
cereal virus
soil
root
wheat
winter wheat
crop rotation
long-term monoculture
Opis:: The species structure of plant parasitic nematode populations from the rhizosphere of winter wheat grown with crop rotation or in 48-year-old monoculture was analyzed and compared. Dominating species: Bitylenchus dubius, Merlinius microdorus, Paratylenchus neglectus and Heterodera avenae, in monoculture plots, had higher populations than in crop rotation plots. Heterodera avenae eggs and larvae were infected by pathogenic fungi in 68% of the monoculture crops (vs. 65–66% of the cysts from crop rotation), 12–20% of Paratylenchus sp. specimens were colonized by bacteria, mainly by Bacillus penetrans. This study shows nematological changes occurring in long-term wheat breeding, thus providing additional information necessary to fight dangerous viral vectors of the examined cereal.
Źródło:: Journal of Plant Protection Research; 2018, 58, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants
Autorzy:: Yazdi, S.E.
Mulabisana, J.
Prinsloo, G.
Cloete, M.
Kritzinger, Q.
Powiązania:: https://bibliotekanauki.pl/articles/66680.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: plant disease
Solanaceae
tobacco plant
cardiac glycoside
antiphytoviral activity
potato virus Y
Potyvirus
Potyviridae
Opis:: The tuber necrotic strain of Potato virus Y (PVYNTN) causes widespread disease and has severe negative effects on the growth and yields of plants, especially those of the Solanaceae family. The consequences of residual toxicity and non-biodegradation of synthetic chemicals and pollution of the environment has led to investigations into new non-toxic and biological treatments to control plant viral diseases. Ethanolic extracts of Bowiea volubilis (bulbs), Cotyledon orbiculata (leaves), Gomphocarpus fruticosus (leaves), Merwilla plumbea (dry and fresh bulbs), Nerium oleander (leaves), and the fruits and leaves of Strophanthus speciosus, were evaluated against PVYNTN in vivo and in vitro. At a concentration of 20 mg · ml−1, ethanolic extracts of Strophanthus speciosus (leaves) and fruits (50 mg · ml−1) significantly reduced the expression of PVYNTN symptoms on tobacco plants in vitro without affecting the normal growth and development of the plant. Similarly, at 50 mg · ml−1, N. oleander, C. orbiculata and B. volubilis (fresh bulbs) and S. speciousus leaves at 20 mg · ml−1 extracts showed significant differences in PVYNTN symptoms in the in vivo experiment. Strophanthus speciosus leaf and fruit extracts showed significant inhibition in the in vitro and in vivo assays and demonstrated that S. speciosus has potential to be used as an antiphytoviral treatment.
Źródło:: Journal of Plant Protection Research; 2018, 58, 4
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Molecular identification of Candidatus Phytoplasma spp. associated with Sophora yellow stunt in Iran
Autorzy:: Allahverdi, T.
Rahimian, H.
Rastgou, M.
Powiązania:: https://bibliotekanauki.pl/articles/66588.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: molecular identification
phytoplasma symptom
sophora plant
Sophora alapecuroides
yellow stunt virus
leaf
yellowing
Iran
Opis:: In the spring of 2012, sophora (Sophora alopecuroides L.) plants showing symptoms of leaf yellowing, little leaves and stunting were observed in Firooz-kuh (Tehran province), Sari (Mazandaran province) and Urmia (West Azerbaijan province) in Iran. Symptomatic plants from the three locations were subjected to nested polymerase chain reaction (PCR) to amplify 16SrRNA using primer pair P1/P7 followed by primer pair R16F2n/R16R2. Th e amplicons were purifi ed, sequenced and the nucleotide sequences were analyzed by virtual restriction fragment length polymorphism (RFLP). Th e phytoplasmas associated with the yellows disease were identifi ed as members of the 16SrIX group (Candidatus Phytoplasma phoenicium) and the 16SrXII group (Candidatus Phytoplasma solani). Th e two phytoplasmas were placed in 16SrIX-C and 16SrXII-A subgroups, respectively, in constructed phylogenetic trees. Th is is the fi rst report on sophora yellows associated with Candidatus Phytoplasma phoenicium.
Źródło:: Journal of Plant Protection Research; 2017, 57, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Prevalence and phylogenetic analysis of Fig mosaic virus and Fig badnavirus-1 in Iran
Autorzy:: Alimoradian, M.
Rakhshandehroo, F.
Shams-bakhsh, M.
Powiązania:: https://bibliotekanauki.pl/articles/66369.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: prevalence
phylogenetic analysis
Fig mosaic virus
Fig badnavirus-1
Iran
fig tree
plant disease
Opis:: Fig mosaic virus (FMV) and Fig badnavirus-1 (FBV-1) are two of the most important fig infecting viruses. The incidence and distribution of FBV-1 and FMV were determined by testing in PCR 138 asymptomatic and symptomatic samples. These samples were collected from 60 fig gardens and agricultural fields in three provinces of Iran. The fig infecting viruses FBV-1 and FMV, respectively, were detected in 92 (66.6%) and 34 (24.6%) samples collected from all the surveyed fields. Overall, 24 out of 138 (17.3%) samples showed mixed infections. The sequence analysis of a genomic fragment of 922 nt, comprising the entire ORF-2 and part of the 5’ termini of the ORF-3 of 10 selected FBV-1 Iranian isolates from different provinces, and of the type member from GenBank (Acc. No: JF411989), showed a variation ranging from 1 to 3% at nucleotide level and 1% at the amino acid level. The phylogenetic analysis grouped the FBV-1 isolates into two groups, with the Iranian isolates clustered in two distinct subgroups of group I, according to their geographical origin. In our research, the prevalence and sequence analysis of FBV-1 as the only identified DNA virus infecting fig trees, was studied for the first time in Iran.
Źródło:: Journal of Plant Protection Research; 2016, 56, 2
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: The reaction of cabbage (Brassica oleracea L.) breeding lines against turnip mosaic virus
Reakcja linii hodowlanych kapusty (Brassica oleracea L.) na turnip mosaic virus
Autorzy:: Sevik, M.A.
Deligoz, I.
Powiązania:: https://bibliotekanauki.pl/articles/11543331.pdf
Data publikacji:: 2016
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: turnip mosaic virus
TuMV zob.turnip mosaic virus
Potyviridae
plant disease
viral disease
resistance
cabbage
Brassica oleracea
Brassicaceae
breeding line
Opis:: Turnip mosaic virus (TuMV) is the most important and widespread virus infecting brassicas worldwide. In 2013, 2014, and 2015, twenty-three cabbage (Brassica oleracea L.) breeding lines from Black Sea Agricultural Research Institute (BSARI, Samsun, Turkey) were screened for their reaction to TuMV-BA isolate by mechanical inoculation under controlled conditions. On the basis of 0–5 disease rating scale and ELISA, of the lines tested, nine (39.1%) were categorized as highly resistant (Grade 1), one (4.3%) as resistant (Grade 2), seven (30.4%) as moderately resistant (Grade 3), however, two cabbage lines and four lines were found moderately susceptible and susceptible, respectively to TuMV-BA isolate under controlled conditions. The results suggested that Brassica oleracea L. from the BSARI contain valuable breeding lines resistant to TuMV, and the screened breeding lines in the current study could provide promising resistance sources for cabbage breeding.
Turnip mosaic virus (TuMV) jest najważniejszym i najbardziej rozpowszechnionym wirusem zakażającym kapustę. W latach 2013, 2014 i 2015 dwadzieścia trzy linie hodowlane kapusty (Brassica oleracea L.) z Czarnomorskiego Instytutu Badań Rolniczych (BSARI, Samsun, Turcja) przetestowano pod względem ich reakcji na izolat TuMV-BA za pomocą inokulacji mechanicznej w warunkach kontrolowanych. Na podstawie 0–5 skali oceny choroby oraz ELISA stwierdzono, że dziewięć (39,1%) z testowanych linii jest wysoce odporne (39,1%) (stopień 1), jedna (4,3%) jest odporna (stopień 2), a siedem (30,4%) jest umiarkowanie odpornych (stopień 3). Jednak stwierdzono, że – odpowiednio – dwie i cztery linie kapusty są umiarkowanie podatne i podatne na izolat TuMV-BA w warunkach kontrolowanych. Na podstawie wyników badania wnioskuje się, że Brassica oleracea L. z BSARI zawierają wartościowe linie hodowlane odporne na TuMV-BA, a linie hodowlane w niniejszym badaniu mogą być cennym źródłem odporności w uprawie kapusty.
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2016, 15, 4; 111-119
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Detection and molecular characterization of the Iris severe mosaic virus-Ir isolate from Iran
Autorzy:: Nateqi, M.
Habibi, M.K.
Dizadji, A.
Parizad, S.
Powiązania:: https://bibliotekanauki.pl/articles/66139.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: detection
molecular characteristics
iris severe mosaic virus
isolate
phylogenesis
iris
Iridaceae
ornamental plant
Iran
Opis:: Iris belongs to the Iridaceae family. It is one of the most important pharmaceutical and ornamental plants in the world. To assess the potyvirus incidence in natural resources of iris plants in Iran, Antigen Coated-Plate ELISA (ACP-ELISA) was performed on 490 symptomatic rhizomatous iris leaf samples, which detected the potyvirus in 36.7% of the samples. Genomic 3’ end of one mechanically non-transmitted potyvirus isolate, comprising a 3’ untranslated region (390 bp) and C-terminus of the coat protein (CP) gene (459 bp), was amplified by reverse transcription polymerase chain reaction (RT-PCR), which was ligated into pTG19-T vector. The nucleotide sequence of amplicons was compared with related sequences, using Blastn software available at NCBI GenBank, and showed the highest similarity with Iris severe mosaic virus (ISMV) isolates. The nucleotide and deduced amino acid sequence of the CP C-terminus region was more than 83% identical with other ISMV isolates, therefore this isolate was designated as ISMV-Ir. This new ISMV isolate is closely related to the Chinese ISMV-PHz in phylogenetic analysis, based on the partial nucleotide and deduced amino acid sequence of the CP region. This is the first report of ISMV occurrence on Iris spp. in Iran.
Źródło:: Journal of Plant Protection Research; 2015, 55, 3
1427-4345
Pojawia się w:: Journal of Plant Protection Research
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Expression of hepatitis B core antigen in lettuce and tobacco for advanced plant-derived vaccine formulations
Autorzy:: Pyrski, M.
Kasprzyk, A.
Bogusiewicz, M.
Bociag, P.
Czyz, M.
Pniewski, T.
Powiązania:: https://bibliotekanauki.pl/articles/951208.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
hepatitis B virus
antigen expression
virus-like particle
transgenic tobacco
lettuce plant
Agrobacterium tumefaciens
immunoblot
ELISA test
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: The expression of HBV antigens in transgenic lettuce micropropagated in vitro cultures for the purpose of anti-HBV vaccine
Autorzy:: Czyz, M.
Wyrwa, K.
Bociag, P.
Dembczynski, R.
Marecik, R.
Krajewski, P.
Kapusta, J.
Pniewski, T.
Powiązania:: https://bibliotekanauki.pl/articles/951182.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: conference
hepatitis B virus
vaccine
vaccination
vaccine production
antigen expression
micropropagation
transgenic plant
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Antagonistyczne interakcje między wirusami w infekcjach mieszanych chronią rośliny przed groźnymi chorobami
Autorzy:: Grupa, A.
Powiązania:: https://bibliotekanauki.pl/articles/836089.pdf
Data publikacji:: 2014
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: ochrona roslin
choroby roslin
odpornosc krzyzowa
wirusy
infekcje mieszane
interakcje
wirusowe interakcje antagonistyczne
plant protection
plant disease
cross resistance
virus
mixed infection
interaction
antagonistic viral interaction
Źródło:: Ziemniak Polski; 2014, 24, 1
1425-4263
Pojawia się w:: Ziemniak Polski
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Cross-protection between different pathotypes of Pepino mosaic virus representing Chilean 2 genotype
Ochrona krzyżowa pomiędzy różnymi patotypami wirusa mozaiki Pepino reprezentującymi genotyp chilijski 2
Autorzy:: Hasiów-Jaroszewska, B.
Minicka, J.
Pospieszny, H.
Powiązania:: https://bibliotekanauki.pl/articles/11542844.pdf
Data publikacji:: 2014
Wydawca:: Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:: cross-protection
pathotype
Pepino mosaic virus
genotype
tomato plant
Opis:: Viral cross-protection in plants is a phenomenon, where a mild virus isolate can protect plants against damage caused by a severe challenge isolate of the same virus. It has been used on a large scale in cases where no resistant plants are available. We examined differences in cross-protection between pathotypes of Pepino mosaic virus representing Chilean 2 genotype. The potential of a mild PepMV-P22 isolate to protect tomato against more aggressive challenge isolates causing yellowing and necrotic symptoms was established. The challenge isolates were PepMV-P5-IY (yellowing), PepMV-P19 (necrotic) and PepMV-P22 K67E (artificial necrotic mutant of PepMV-P22 which differ from PepMV-P22 only by a point mutation). Efficient cross-protection was obtained using mild PepMV-P22 against PepMV-P5-IY. After a challenge inoculation with PepMV-P19 or PepMV-P22 K67E symptoms severity were significantly reduced in comparison to non-protected plants; however, necrotic symptoms appeared two months after coinfection. The real-time PCR analysis revealed that the level of accumulation of the necrotic isolate in tomato plants was even 5–7 times higher than that of PepMV-P22.
Zjawisko ochrony krzyżowej (ang. cross-protection) zachodzi tylko pomiędzy izolatami pokrewnymi danego gatunku wirusa. Polega ono na celowym zakażeniu roślin bardzo łagodnym izolatem wirusa (izolat ochronny), aby chronić je przed innym, ostrym izolatem tego samego wirusa (izolat konkurencyjny). W prezentowanej pracy analizowano potencjał wykorzystania łagodnego izolatu (PepMV-P22) wirusa mozaiki pepino (Pepino mosaic virus, PepMV) do ochrony roślin pomidora przeciwko innym izolatom, powodującym zróżnicowane objawy na roślinach. Jako izolaty konkurencyjne wykorzystano: PepMV-P5-IY (żółtaczkowy), PepMV-P19 (nekrotyczny) oraz PepMV-P22 K67E (mutant nekrotyczny, różniący się jedynie pojedynczą mutacją od PepMV-P22). Zjawisko ochrony krzyżowej zachodziło efektywnie w przypadku wykorzystania PepMVP22 przeciwko PepMV-P5-IY. W przypadku PepMV-P19 oraz PepMV-P22 K67E ochrona krzyżowa została przełamana, jednakże symptomy były mniej intensywne i pojawiły się później niż u roślin, u których nie stosowano ochrony krzyżowej. Ponadto analiza realtime PCR wykazała, że akumulacja wirusa w przypadku nekrotycznych wariantów była około 5–7 razy większa w porównaniu z łagodnym izolatem wirusa.
Źródło:: Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 5; 177-185
1644-0692
Pojawia się w:: Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:: Biblioteka Nauki