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Wyświetlanie 1-15 z 43
Tytuł:
Tissue variation of mitochondrial oxidative phosphorylation efficiency in cold-acclimated ducklings
Autorzy:
Salin, Karine
Teulier, Loïc
Rey, Benjamin
Rouanet, Jean-Louis
Voituron, Yann
Duchamp, Claude
Roussel, Damien
Powiązania:
https://bibliotekanauki.pl/articles/1040299.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
liver
skeletal muscle
thermogenesis
mitochondrial oxidative phosphorylation
proton conductance
Opis:
We investigated the oxidative phosphorylation efficiency of liver and gastrocnemius muscle mitochondria in thermoneutral and cold-acclimated ducklings. The yield of oxidative phosphorylation was lower in muscle than in liver mitochondria, a difference that was associated with a higher proton conductance in muscle mitochondria. Cold exposure did not affect oxidative phosphorylation efficiency or basal proton leak in mitochondria. We conclude that the basal proton conductance of mitochondria may regulate mitochondrial oxidative phosphorylation efficiency, but is not an important contributor to thermogenic processes in cold-acclimated ducklings.
Źródło:
Acta Biochimica Polonica; 2010, 57, 4; 409-412
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of vanadate on Pichia pastoris growth, protein kinase A activity and ribosomal protein phosphorylation.
Autorzy:
Frajnt, Magdalena
Cytryńska, Małgorzata
Jakubowicz, Teresa
Powiązania:
https://bibliotekanauki.pl/articles/1043701.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
protein phosphorylation
vanadate
Pichia pastoris
PKA
ribosomal proteins
protein kinases
Opis:
It was found that wild type yeast Pichia pastoris can tolerate vanadate concentration as high as 25 mM in the growth medium. Moreover, four vanadate-resistant P. pastoris strains designated JC100/1, JC100/3, JC100/9 and JC100/15 exhibiting tolerance up to 150 mM vanadate were selected. Growth of P. pastoris was correlated with vanadate to vanadyl reduction and its accumulation in the growth medium. In two selected strains, JC100/9 and JC100/15, protein kinase A activity was much higher in comparison to the wild type strain even without vanadate addition to the growth medium. Moreover, in the presence of vanadate, protein kinase A activity was significantly increased in the wild type and the vanadate-resistant JC100/1 and JC100/3 strains. It was also found that phosphorylation of a 40 kDa protein associated with ribosomes occured in all vanadate-resistant strains from the logarithmic, while in the wild type strain from the stationary growth phase. From the presented results it can be concluded that a protein kinase A signalling pathway(s) might be involved in the mechanism of P. pastoris vanadate resistance. The results also indicate a possible role of the 40 kDa protein in protection of P. pastoris against vanadate toxicity.
Źródło:
Acta Biochimica Polonica; 2002, 49, 4; 959-968
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of cAMP and cGMP on the activity and substrate specificity of protein kinase A from methylotrophic yeast Pichia pastoris.
Autorzy:
Frajnt, Magdalena
Cytryńska, Małgorzata
Jakubowicz, Teresa
Powiązania:
https://bibliotekanauki.pl/articles/1043395.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
protein phosphorylation
cAMP
PKA
cGMP
yeast
Opis:
Cyclic AMP dependent protein kinase (PKA) from Pichia pastoris yeast cells was found to be activated by either cAMP or cGMP. Analogs of cAMP such as 8-chloro-cAMP and 8-bromo-cAMP were as potent as cAMP in PKA activation while N6,2'-O-dibutyryl-cAMP did not stimulate the enzyme activity. It was shown that protamine sulfate was almost equally phosphorylated in the presence of 1-2 × 10-6 M cAMP or cGMP while other substrates such as Kemptide, ribosomal protein S6, were phosphorylated to a lower extent in the presence of cGMP. It was demonstrated that pyruvate kinase is a substrate of PKA which co-purified with the P. pastoris enzyme. Moreover, pyruvate kinase was phosphorylated by PKA in the presence of cAMP and cGMP to comparable levels.
Źródło:
Acta Biochimica Polonica; 2003, 50, 4; 1111-1118
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Starches Modified by Combination of Phosphorylation and High-Voltage Electrical Discharge (HVED) Treatment
Autorzy:
Grgić, Ivanka
Grec, Marijana
Gryszkin, Artur
Zięba, Tomasz
Kopjar, Mirela
Ačkar, Đurđica
Jozinović, Antun
Miličević, Borislav
Zavadlav, Sandra
Babić, Jurislav
Powiązania:
https://bibliotekanauki.pl/articles/1363283.pdf
Data publikacji:
2021
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
cereal starch
tuber starch
HVED
phosphorylation
physicochemical properties
Opis:
Starch is extensively used in the food industry as a texture modifier, a fat substitute, and in other applications. To optimise starch functional properties for specific use, it is subjected to various modifications. High-voltage electrical discharge (HVED) treatment, as a non-thermal and rapid process, was applied in this research as a single method and in combination with phosphorylation in order to explore its potential for improving starch physicochemical properties. Maize, wheat, potato, and tapioca starches were modified, and Na5P3O10 and Na2HPO4 were used for phosphorylation. Starch gelatinisation parameters (by DSC); paste clarity; and contents of amylose, damaged starch, and resistant starch were determined; and FTIR-ATR spectra were recorded. All modifications reduced the enthalpy of gelatinisation and decreased contents of amylose, resistant starch, and damaged starch. The effect of the HVED treatment on starch properties depended on starch type and combinations with chemicals. HVED could act as an aid in the starch phosphorylation process since the properties analysed were more effectively improved when HVED was combined with phosphorylation than by phosphorylation alone.
Źródło:
Polish Journal of Food and Nutrition Sciences; 2021, 71, 1; 79-88
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Spektrometria mas w analizie białek i peptydów : znaczniki jonizacyjne
Mass spectrometry in analysis of peptides and proteins : ionization markers
Autorzy:
Bąchor, R.
Biernat, M.
Cebrat, M.
Kijewska, M.
Kluczyk, A.
Kuczer, M.
Paluch, A.
Waliczek, M.
Wierzbicka, M.
Stefanowicz, P.
Szewczuk, Z.
Powiązania:
https://bibliotekanauki.pl/articles/171509.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Chemiczne
Tematy:
spektrometria mas
modyfikacje potranslacyjne
znaczniki jonizacyjne
fosforylacja
glikacja
biblioteki kombinatoryczne
mass spectrometry
post-translational modifications
ionization markers
phosphorylation
glycation
combinatorial libraries
Opis:
High sensitivity, accuracy, and ability to provide structural information makes mass spectrometry (MS) the method of choice for both qualitative and quantitative analysis in proteome research. Peptide sequencing by tandem mass spectrometry (MS/MS) was successfully applied to discover new peptide sequences and modifications. Insufficient ionization of some peptides is one of the main limitations of MS- based peptide identification. The development of sensitive detection techniques for the efficient analysis of such samples is very important. Differences in ionizability cause difficulties in quantification studies, which could be overcome by derivatization of peptides to improve both the detectability and the selectivity of an analysis. Incorporation of ionization markers and isotopic labels (particularly the isobaric tags) is often used for this reason. Isobaric labeling reagents (including commercially available iTRAQ, TMT, DiLeu and DiART) have found a wide application in quantitative proteomics. Mass spectrometry is a very good tool for the determination of posttranslational modifications (PTMs), but the modified proteins are usually present in low concentrations. The development of ionization tags specific to a particular PTM and suitable for sensitive analysis of the modified proteins is required. For the analysis of phosphorylated peptides, a combination of β-elimination and the reaction of resulting α,β-dehydroamino acid residues with the nucleophilic thiol group could be used to detect a labile PTM. Such reaction may be used to introduce derivatizing reagents at the original site of phosphorylation, to enhance ionization in MS analysis. Glycation and glycosylation of proteins are other very important PTMs associated with many natural processes as well as diseases. We have designed and synthesized bifunctional quaternary ammonium salt derivatives of phenylboronic acids for selective detection of carbohydrates and peptide-derived Amadori products by ESI-MS. The attachment of a fixed charge (e.g. in a form of a quaternary ammonium salt) to the amino groups in peptides leads to the enhancement of a precursor ion signal in mass spectra. We have developed several new QAS-containing ionization reagents including bicyclic tags with DABCO, ABCO or azoniaspiro groups. It is worth noting that 2,4,6-substituted pyrylium salts react with amino groups in peptides introducing a stable positive charge and improve peptide detection by MS. The newly developed ionization tags were successfully applied for the analysis of OBOC combinatorial libraries as well as for studying possible biomarkers of preeclampsia, a pregnancy disorder.
Źródło:
Wiadomości Chemiczne; 2018, 72, 7-8; 609-633
0043-5104
2300-0295
Pojawia się w:
Wiadomości Chemiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Short-term regulation of the mammalian pyruvate dehydrogenase complex
Autorzy:
Strumiło, Sławomir
Powiązania:
https://bibliotekanauki.pl/articles/1041314.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
pyruvate dehydrogenase complex
thiamine diphosphate
phosphorylation
dephosphorylation
regulation
allosteric effect
Opis:
In this minireview the main mechanism of control of mammalian pyruvate dehydrogenase complex (PDHC) activity by phosphorylation-dephosphorylation is presented in the first place. The information recently obtained in several laboratories includes new data about isoforms of the PDH converting enzymes (kinase and phosphatase) and their action in view of short-term regulation of PDHC. Moreover, interesting influence of exogenous thiamine diphosphate (TDP) and some divalent cations, especially Mn2+, on the kinetic parameters of PDHC saturated with endogenous tightly bound TDP, is discussed. This influence causes a shortening of the lag-phase of the catalyzed reaction and a strong decrease of the Km value of PDHC mainly for pyruvate. There are weighty arguments that the effects have an allosteric nature. Thus, besides reversible phosphorylation, also direct manifold increase of mammalian PDHC affinity for the substrate by cofactors seems an important aspect of its regulation.
Źródło:
Acta Biochimica Polonica; 2005, 52, 4; 759-764
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
SDS/PAGE characteristics of protein kinases tightly associated with chick embryo brain ribosomes
Autorzy:
Sanecka-Obacz, Maria
Powiązania:
https://bibliotekanauki.pl/articles/1044449.pdf
Data publikacji:
1999
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
protein phosphorylation
chick brain development
ribosomal kinases
CK2
PKA
CK1
fetal brain
brain ribosomes
Opis:
Protein kinases tightly associated with chick embryo brain ribosomes washed with Triton X-100 and KCl were characterized by their ability to phosphorylate ribosomes and two exogenous substrates, histone IIA and casein. c-AMP-dependent kinase (PKA) and casein kinases (CK1, CK2) were examined in the presence of specific modulators by SDS/ PAGE followed by  renaturation in gel assay according to Kameshita & Fujisawa (Anal. Biochem. 1989, 183, 139-143). Basing on these data it can be presumed that PKA activity increases, but the levels of CK2 and CK1 decrease during chick embryo development.
Źródło:
Acta Biochimica Polonica; 1999, 46, 4; 911-917
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Role of spruce respiratory burst oxidase homolog (RBOH) during lignin formation in Norway spruce
Autorzy:
Nickolov, K.
Laitinen, T.
Haggman, H.
Teeri, T.
Karkonen, A.
Powiązania:
https://bibliotekanauki.pl/articles/80939.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
Norway spruce
respiratory burst
lignin formation
hydrogen peroxide
NADPH oxidase
phosphorylation
protein-protein interaction
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rabbit muscle fructose-1,6-bisphosphatase is phosphorylated in vivo.
Autorzy:
Rakus, Dariusz
Zarzycki, Marek
Dzugaj, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1043652.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
aldolase
muscle
phosphorylation
AMP
fructose-1,6-bisphosphatase
Opis:
Phosphorylated fructose-1,6-bisphosphatase (FBPase) was isolated from rabbit muscle in an SDS/PAGE homogeneous form. Its dephosphorylation with alkaline phosphatase revealed 2.8 moles of inorganic phosphate per mole of FBPase. The phosphorylated FBPase (P-FBPase) differs from the dephosphorylated enzyme in terms of its kinetic properties like Km and kcat, which are two times higher for the phosphorylated FBPase, and in the affinity for aldolase, which is three times lower for the dephosphorylated enzyme. ephosphorylated FBPase can be a substrate for protein kinase A and the amount of phosphate incorporated per FBPase monomer can reach 2-3 molecules. Since interaction of muscle aldolase with muscle FBPase results in desensitisation of the latter toward AMP inhibition (Rakus & Dzugaj, 2000, Biochem. Biophys. Res. Commun. 275, 611-616), phosphorylation may be considered as a way of muscle FBPase activity regulation.
Źródło:
Acta Biochimica Polonica; 2003, 50, 1; 115-121
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prp4 kinase is required for proper segregation of chromosomes during meiosis in Schizosaccharomyces pombe
Autorzy:
Pozgajova, Miroslava
Cipak, Lubos
Trakovicka, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1039511.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Prp4 protein kinase
S. pombe
meiosis
segregation
protein phosphorylation
Opis:
Chromosome segregation during meiosis is a complex process, which leads to production of four haploid gametes from two precursor cells. Reversible phosphorylation of proteins plays a crucial role in this process. The Schizosaccharomyces pombe Prp4 is an essential serine/threonine protein kinase, which belongs to the Clk/Sty family. To study the role of Prp4 in meiosis, we analysed chromosome segregation in a strain carrying conditional analog-sensitive allele of Prp4 protein kinase (prp4-as2). Our data show, that Prp4 protein kinase plays important role in chromosome segregation during meiosis, as revealed by enhanced missegregation of chromosomes in prp4-as2 mutant cells.
Źródło:
Acta Biochimica Polonica; 2013, 60, 4; 871-873
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Protein phosphatase 2A: Variety of forms and diversity of functions
Autorzy:
Lechward, Katarzyna
Awotunde, Olubunmi
Świątek, Wojciech
Muszyńska, Grażyna
Powiązania:
https://bibliotekanauki.pl/articles/1044037.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
protein phosphatase 2A
signal transduction
protein-protein interactions
reversible phosphorylation
cell cycle
carcinogenesis.
Opis:
Protein phosphatase 2A (PP2A) comprises a diverse family of phosphoserine-and phosphothreonine-specific phosphatases present in all eukaryotic cells. All forms of PP2A contain a catalytic subunit (PP2Ac) which forms a stable complex with the structural subunit PR65/A. The heterodimer PP2Ac-PR65/A associates with regulatory proteins, termed variable subunits, in order to form trimeric holoenzymes attributed with distinct substrate specificity and targeted to different subcellular compartments. PP2Ac activity can be modulated by reversible phosphorylation on Tyr307 and methylation on C-terminal Leu309. Studies on PP2A have shown that this enzyme may be implicated in the regulation of metabolism, transcription, RNA splicing, translation, differentiation, cell cycle, oncogenic transformation and signal transduction.
Źródło:
Acta Biochimica Polonica; 2001, 48, 4; 921-933
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Protein kinases CKI and CKII are implicated in modification of ribosomal proteins of the yeast Trichosporon cutaneum
Autorzy:
Wojda, Iwona
Cytryńska, Małgorzata
Frajnt, Magdalena
Jakubowicz, Teresa
Powiązania:
https://bibliotekanauki.pl/articles/1043700.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
CKII
CKI
phosphorylation
ribosomal phosphoproteins
Trichosporon cutaneum
Opis:
Phosphorylation of acidic ribosomal proteins P1/P2-P0 is a common phenomenon in eukaryotic organisms. It was found previously that in Trichosporon cutaneum, unlike in other yeast species, in addition to the two acidic ribosomal proteins, two other proteins of 15 kDa and 19 kDa of the small ribosomal subunit were phosphorylated. Here we describe two protein kinases: CKI and CKII, which are engaged in the modification of T. cutaneum ribosomal proteins. The acidic ribosomal proteins and the protein of 19 kDa were modified by CKII associated with ribosomes, while the protein of 15 kDa was modified by CKI. Protein kinase CKI was purified from cell-free extract (CKIC) and from ribosomal fraction (CKIR). The molecular mass of CKIC was established at 33 kDa while that of CKIR at 35-37 kDa. A protein of 40 kDa copurified with CKIR but not CKIC. Heparin significantly increased 40 kDa protein phosphorylation level by CKIR. Microsequencing analysis revealed the presence of CKI recognition motifs in the N-terminal fragment of the 40 kDa protein.
Źródło:
Acta Biochimica Polonica; 2002, 49, 4; 947-957
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
PP2A controls methionine metabolism elicited by intracellular H2O2
Autorzy:
Trotta, A.
Li, S.
Mhamdi, A.
Ravanel, S.
Noctor, G.
Kangasjarvi, S.
Powiązania:
https://bibliotekanauki.pl/articles/80912.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
intracellular signalling
plant cell
reactive oxygen species
protein
post-translational modification
phosphorylation
methionine
hydrogen peroxide
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phosphorylation sites of HER2/c-erbB-2: role in cell growth and in disease
Autorzy:
Khurshid, Rukhshan
Saleem, Mahjabeen
Gul-e-Raana, -
Akhthar, Muhammad
Powiązania:
https://bibliotekanauki.pl/articles/1039198.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
phosphorylation sites
HER2/c-erbB-2
cell growth and disease
Opis:
The protein kinase c-erbB-2 belongs to the family of receptor tyrosine kinase and is involved in oncogenesis. The present study predicts different phosphorylation sites of HER2/c-erbB-2 which are important in preventing or developing cancer, especially breast cancer. Sequence homology showed highest homology (77%) with epidermal growth factor receptor kinase domain. According to PROSITE search result, active sites of c-erbB-2 are N-lobe (glycine rich phosphate binding loop). Catalytic loop with presumptive catalytically active of Asp108 is phosphorylated by tyrosine protein kinase. A-loop, activation loop, becomes phosphorylated and activates the substrate binding. The study strengthens our knowledge regarding HER2 signaling by the detection of uncharacterized signaling proteins, establishing phosphorylation of an activation loop and helps us to make assumptions about the role of such previously unidentified proteins. On the basis of importance of HER2 in breast cancer as well as in other diseases, this study provides fruitful information for designing new therapeutic strategies.
Źródło:
Acta Biochimica Polonica; 2014, 61, 4; 699-703
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phosphorylation of basic amino acid residues in proteins: important but easily missed
Autorzy:
Cieśla, Joanna
Frączyk, Tomasz
Rode, Wojciech
Powiązania:
https://bibliotekanauki.pl/articles/1039902.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
basic amino acids
posttranslational modification
phosphorylation
acid-labile
base-stable
phosphoramidate
Opis:
Reversible phosphorylation is the most widespread posttranslational protein modification, playing regulatory role in almost every aspect of cell life. The majority of protein phosphorylation research has been focused on serine, threonine and tyrosine that form acid-stable phosphomonoesters. However, protein histidine, arginine and lysine residues also may undergo phosphorylation to yield acid-labile phosphoramidates, most often remaining undetected in conventional studies of protein phosphorylation. It has become increasingly evident that acid-labile protein phosphorylations play important roles in signal transduction and other regulatory processes. Beside acting as high-energy intermediates in the transfer of the phosphoryl group from donor to acceptor molecules, phosphohistidines have been found so far in histone H4, heterotrimeric G proteins, ion channel KCa3.1, annexin 1, P-selectin and myelin basic protein, as well as in recombinant thymidylate synthase expressed in bacterial cells. Phosphoarginines occur in histone H3, myelin basic protein and capsidic protein VP12 of granulosis virus, whereas phospholysine in histone H1. This overview of the current knowledge on phosphorylation of protein basic amino-acid residues takes into consideration its proved or possible roles in cell functioning. Specific requirements of studies on acid-labile protein phosphorylation are also indicated.
Źródło:
Acta Biochimica Polonica; 2011, 58, 2; 137-148
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
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