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    Wyświetlanie 1-43 z 43
    Tytuł:
    Inhibition of mitochondrial bioenergetics: the effects on structure of mitochondria in the cell and on apoptosis.
    Autorzy:
    Lyamzaev, Konstantin
    Izyumov, Denis
    Avetisyan, Armine
    Yang, Fuyu
    Pletjushkina, Olga
    Chernyak, Boris
    Powiązania:
    https://bibliotekanauki.pl/articles/1043303.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    oxidative phosphorylation
    inhibitors
    mitochondria
    apoptosis
    Opis:
    The effects of specific inhibitors of respiratory chain, FoF1ATP synthase and uncouplers of oxidative phosphorylation on survival of carcinoma HeLa cells and on the structure of mitochondria in the cells were studied. The inhibitors of respiration (piericidingg, antimycin, myxothiazol), the F1-component of ATP synthase (aurovertin) and uncouplers (DNP, FCCP) did not affect viability of HeLa cells, apoptosis induced by TNF or staurosporin and the anti-apoptotic action of Bcl-2. Apoptosis was induced by combined action of respiratory inhibitors and uncouplers indicating possible pro-apoptotic action of reactive oxygen species (ROS) generated by mitochondria. Short-term incubation of HeLa cells with the mitochondrial inhibitors and 2-deoxyglucose followed by 24-48 h recovery resulted in massive apoptosis. Apoptosis correlated to transient (3-4 h) and limited (60-70%) depletion of ATP. More prolonged or more complete transient ATP depletion induced pronounced necrosis. The inhibitors of respiration and uncouplers caused fragmentation of tubular mitochondria and formation of small round bodies followed by swelling. These transitions were not accompanied with release of cytochrome c into the cytosol and were fully reversible. The combined effect of respiratory inhibitors and uncouplers developed more rapidly indicating possible involvement of ROS generated by mitochondria. More prolonged (48-72 h) incubation with this combination of inhibitors caused clustering and degradation of mitochondria.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 2; 553-562
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Myocardial remodeling in rats with metabolic syndrome: role of Rho-kinase mediated insulin resistance
    Autorzy:
    Li, Chuan-Bao
    Li, Xiao-Xing
    Chen, Yu-Guo
    Gao, Hai-Qing
    Bao, Cheng-Mei
    Liu, Xiang-Qun
    Bu, Pei-Li
    Zhang, Juan
    Zhang, Yun
    Ji, Xiao-Ping
    Powiązania:
    https://bibliotekanauki.pl/articles/1039743.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Rat
    Metabolism
    Myocardium
    Phosphorylation
    Insulin resistance
    Opis:
    Insulin resistance (IR) plays a critical role in metabolic syndrome (MS). Previous studies have demonstrated that activated ROCK is increased in MS patients. However, the effect of Rho-kinase (ROCK) on IR has not been definitely determined. Thus, the aims of the present study were to determine whether ROCK activation induces IR or affects myocardial structure and function, as well as the possible mechanisms underlying this process. Wistar rats fed high fat, high glucose and high salt diet sewed as model of MS and we used transmission electron microscopy, echocardiogram technology, and terminal deoxynucleotidyl transferase-mediated DNA nick-end labeling staining to identify any myocardial damage. The protein levels of MYPT-1 (characteristic of ROCK activation), IRS-1 and AKT were analyzed by immunohistochemistry and Western blotting. In hearts from MS rats, we found increased protein levels of phospho-MYPT-1 and phospho-IRS-1 (Ser307) and decreased phospho-AKT compared to levels in normal rats. In conclusion, the results suggest that ROCK-mediated IR is involved in the development of myocardial impairments in MS rats and that this effect is mediated probably via the IRS-1/PI3-kinase/AKT pathway.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 2; 249-254
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Modulation of the human preadipocyte mitochondrial activity by beta-carotene
    Autorzy:
    Śliwa, Agnieszka
    Góralska, Joanna
    Czech, Urszula
    Gruca, Anna
    Polus, Anna
    Zapała, Barbara
    Dembińska-Kieć, Aldona
    Powiązania:
    https://bibliotekanauki.pl/articles/1039768.pdf
    Data publikacji:
    2012
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    oxidative phosphorylation
    preadipocytes
    mitochondria
    beta-carotene
    Opis:
    Increased ROS generation by the overload by metabolic substrates mitochondria paralleled by decrease of antioxidant activity are typical events found in metabolic syndrome and diabetes type 2. Metabolites of beta-carotene (BC) such as retinoic acid (RA), as well as low concentration of reactive oxygen species (ROS) modify the mitochondrial bioenergetic function. The aim of the study was to investigate the effect of beta-carotene on mitochondrial activity in human preadipocytes. BC used in concentrations, 10 or 30 µM, decreased mitochondrial membrane potential, inhibited mitochondrial respiration and decreased cellular ATP content. We conclude, that BC, the known antioxidant may decrease oxidative phosphorylation capacity of mitochondria.
    Źródło:
    Acta Biochimica Polonica; 2012, 59, 1; 39-41
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Characterization of dual specificity protein kinase from maize seedlings.
    Autorzy:
    Trojanek, Joanna
    Klimecka, Maria
    Fraser, Anna
    Dobrowolska, Grażyna
    Muszyńska, Grażyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1041540.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    dual specificity kinase
    tyrosine phosphorylation
    maize
    Opis:
    A protein kinase of 57 kDa, able to phosphorylate tyrosine in synthetic substrates pol(Glu4,Tyr1) and a fragment of Src tyrosine kinase, was isolated and partly purified from maize seedlings (Zea mays). The protein kinase was able to phosphorylate exogenous proteins: enolase, caseins, histones and myelin basic protein. Amino acid analysis of phosphorylated casein and enolase, as well as of phosphorylated endogenous proteins, showed that both Tyr and Ser residues were phosphorylated. Phosphotyrosine was also immunodetected in the 57 kDa protein fraction. In the protein fraction there are present 57 kDa protein kinase and enolase. This co-purification suggests that enolase can be an endogenous substrate of the kinase. The two proteins could be resolved by two-dimensional electrophoresis. Specific inhibitors of typical protein-tyrosine kinases had essentially no effect on the activity of the maize enzyme. Staurosporine, a nonspecific inhibitor of protein kinases, effectively inhibited the 57 kDa protein kinase. Also, poly L-lysine and heparin inhibited tyrosine phosphorylation by 57 kDa maize protein kinase. The substrate and inhibitor specificities of the 57 kDa maize protein kinase phosphorylating tyrosine indicate that it is a novel plant dual-specificity protein kinase.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 3; 635-647
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of cAMP and cGMP on the activity and substrate specificity of protein kinase A from methylotrophic yeast Pichia pastoris.
    Autorzy:
    Frajnt, Magdalena
    Cytryńska, Małgorzata
    Jakubowicz, Teresa
    Powiązania:
    https://bibliotekanauki.pl/articles/1043395.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    protein phosphorylation
    cAMP
    PKA
    cGMP
    yeast
    Opis:
    Cyclic AMP dependent protein kinase (PKA) from Pichia pastoris yeast cells was found to be activated by either cAMP or cGMP. Analogs of cAMP such as 8-chloro-cAMP and 8-bromo-cAMP were as potent as cAMP in PKA activation while N6,2'-O-dibutyryl-cAMP did not stimulate the enzyme activity. It was shown that protamine sulfate was almost equally phosphorylated in the presence of 1-2 × 10-6 M cAMP or cGMP while other substrates such as Kemptide, ribosomal protein S6, were phosphorylated to a lower extent in the presence of cGMP. It was demonstrated that pyruvate kinase is a substrate of PKA which co-purified with the P. pastoris enzyme. Moreover, pyruvate kinase was phosphorylated by PKA in the presence of cAMP and cGMP to comparable levels.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 4; 1111-1118
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    NH4plus -mediated protein phosphorylation in rice roots
    Autorzy:
    Zhu, X.F.
    Cai, W.H.
    Jung, J.H.
    Xuan, Y.H.
    Powiązania:
    https://bibliotekanauki.pl/articles/19339.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    ammonium
    protein phosphorylation
    rice
    root
    phosphoproteomics
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2015, 57, 2
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Liver mitochondria and insulin resistance
    Autorzy:
    Vial, Guillaume
    Dubouchaud, Hervé
    Leverve, Xavier
    Powiązania:
    https://bibliotekanauki.pl/articles/1040285.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    oxidative phosphorylation
    insulin resistance
    mitochondria
    membrane potential
    Opis:
    With a steadily increasing prevalence, insulin resistance (IR) is a major public health issue. This syndrome is defined as a set of metabolic dysfunctions associated with, or contributing to, a range of serious health problems. These disorders include type 2 diabetes, metabolic syndrome, obesity, and non-alcoholic steatohepatitis (NASH). According to the literature in the field, several cell types like β-cell, myocyte, hepatocyte and/or adipocyte, as well as related complex signaling environment involved in peripheral insulin sensitivity are believed to be central in this pathology. Because of the central role of the liver in the whole-body energy homeostasis, liver insulin sensitivity and its potential relationship with mitochondrial oxidative phosphorylation appear to be crucial. The following short review highlights how liver mitochondria could be implicated in IR and should therefore be considered as a specific therapeutic target in the future.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 4; 389-392
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Protein kinases CKI and CKII are implicated in modification of ribosomal proteins of the yeast Trichosporon cutaneum
    Autorzy:
    Wojda, Iwona
    Cytryńska, Małgorzata
    Frajnt, Magdalena
    Jakubowicz, Teresa
    Powiązania:
    https://bibliotekanauki.pl/articles/1043700.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    CKII
    CKI
    phosphorylation
    ribosomal phosphoproteins
    Trichosporon cutaneum
    Opis:
    Phosphorylation of acidic ribosomal proteins P1/P2-P0 is a common phenomenon in eukaryotic organisms. It was found previously that in Trichosporon cutaneum, unlike in other yeast species, in addition to the two acidic ribosomal proteins, two other proteins of 15 kDa and 19 kDa of the small ribosomal subunit were phosphorylated. Here we describe two protein kinases: CKI and CKII, which are engaged in the modification of T. cutaneum ribosomal proteins. The acidic ribosomal proteins and the protein of 19 kDa were modified by CKII associated with ribosomes, while the protein of 15 kDa was modified by CKI. Protein kinase CKI was purified from cell-free extract (CKIC) and from ribosomal fraction (CKIR). The molecular mass of CKIC was established at 33 kDa while that of CKIR at 35-37 kDa. A protein of 40 kDa copurified with CKIR but not CKIC. Heparin significantly increased 40 kDa protein phosphorylation level by CKIR. Microsequencing analysis revealed the presence of CKI recognition motifs in the N-terminal fragment of the 40 kDa protein.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 4; 947-957
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Rabbit muscle fructose-1,6-bisphosphatase is phosphorylated in vivo.
    Autorzy:
    Rakus, Dariusz
    Zarzycki, Marek
    Dzugaj, Andrzej
    Powiązania:
    https://bibliotekanauki.pl/articles/1043652.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    aldolase
    muscle
    phosphorylation
    AMP
    fructose-1,6-bisphosphatase
    Opis:
    Phosphorylated fructose-1,6-bisphosphatase (FBPase) was isolated from rabbit muscle in an SDS/PAGE homogeneous form. Its dephosphorylation with alkaline phosphatase revealed 2.8 moles of inorganic phosphate per mole of FBPase. The phosphorylated FBPase (P-FBPase) differs from the dephosphorylated enzyme in terms of its kinetic properties like Km and kcat, which are two times higher for the phosphorylated FBPase, and in the affinity for aldolase, which is three times lower for the dephosphorylated enzyme. ephosphorylated FBPase can be a substrate for protein kinase A and the amount of phosphate incorporated per FBPase monomer can reach 2-3 molecules. Since interaction of muscle aldolase with muscle FBPase results in desensitisation of the latter toward AMP inhibition (Rakus & Dzugaj, 2000, Biochem. Biophys. Res. Commun. 275, 611-616), phosphorylation may be considered as a way of muscle FBPase activity regulation.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 1; 115-121
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cloning and characterization of the gene encoding ribosomal P0 phosphoprotein from Neurospora crassa.
    Autorzy:
    Krokowski, Dawid
    Tchórzewski, Marek
    Grankowski, Nikodem
    Powiązania:
    https://bibliotekanauki.pl/articles/1043800.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    phosphorylation
    cloning
    ribosomal P0 protein
    Neurospora crassa
    Opis:
    A gene for ribosomal protein P0 that belongs to the family of ribosomal P proteins was isolated from a Neurospora crassa cDNA library, using polyclonal antibodies against recombinant P0 protein from Saccharomyces cerevisiae. This is the first gene for ribosomal P0 protein to be cloned from filamentous fungi. The derived P0 protein sequence has a strong homology to other eukaryotic P0 proteins; yet, there is a notable alteration in the conservative C-terminal region, placing this protein among the unique sequences from protozoan parasites.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 1; 11-18
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Is MLC phosphorylation essential for the recovery from ROCK inhibition in glioma C6 cells?
    Autorzy:
    Korczyński, Jarosław
    Sobierajska, Katarzyna
    Krzemiński, Patryk
    Wasik, Anna
    Wypych, Dorota
    Pomorski, Paweł
    Kłopocka, Wanda
    Powiązania:
    https://bibliotekanauki.pl/articles/1039964.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    RhoA
    myosin II
    calcium signaling
    actin
    MLC phosphorylation
    Opis:
    Inhibition of Rho-associated protein kinase (ROCK) activity in glioma C6 cells induces changes in actin cytoskeleton organization and cell morphology similar to those observed in other types of cells with inhibited RhoA/ROCK signaling pathway. We show that phosphorylation of myosin light chains (MLC) induced by P2Y2 receptor stimulation in cells with blocked ROCK correlates in time with actin cytoskeleton reorganization, F-actin redistribution and stress fibers assembly followed by recovery of normal cell morphology. Presented results indicate that myosin light-chain kinase (MLCK) is responsible for the observed phosphorylation of MLC. We also found that the changes induced by P2Y2 stimulation in actin cytoskeleton dynamics and morphology of cells with inhibited ROCK, but not in the level of phosphorylated MLC, depend on the presence of calcium in the cell environment.
    Źródło:
    Acta Biochimica Polonica; 2011, 58, 1; 125-130
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Starches Modified by Combination of Phosphorylation and High-Voltage Electrical Discharge (HVED) Treatment
    Autorzy:
    Grgić, Ivanka
    Grec, Marijana
    Gryszkin, Artur
    Zięba, Tomasz
    Kopjar, Mirela
    Ačkar, Đurđica
    Jozinović, Antun
    Miličević, Borislav
    Zavadlav, Sandra
    Babić, Jurislav
    Powiązania:
    https://bibliotekanauki.pl/articles/1363283.pdf
    Data publikacji:
    2021
    Wydawca:
    Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
    Tematy:
    cereal starch
    tuber starch
    HVED
    phosphorylation
    physicochemical properties
    Opis:
    Starch is extensively used in the food industry as a texture modifier, a fat substitute, and in other applications. To optimise starch functional properties for specific use, it is subjected to various modifications. High-voltage electrical discharge (HVED) treatment, as a non-thermal and rapid process, was applied in this research as a single method and in combination with phosphorylation in order to explore its potential for improving starch physicochemical properties. Maize, wheat, potato, and tapioca starches were modified, and Na5P3O10 and Na2HPO4 were used for phosphorylation. Starch gelatinisation parameters (by DSC); paste clarity; and contents of amylose, damaged starch, and resistant starch were determined; and FTIR-ATR spectra were recorded. All modifications reduced the enthalpy of gelatinisation and decreased contents of amylose, resistant starch, and damaged starch. The effect of the HVED treatment on starch properties depended on starch type and combinations with chemicals. HVED could act as an aid in the starch phosphorylation process since the properties analysed were more effectively improved when HVED was combined with phosphorylation than by phosphorylation alone.
    Źródło:
    Polish Journal of Food and Nutrition Sciences; 2021, 71, 1; 79-88
    1230-0322
    2083-6007
    Pojawia się w:
    Polish Journal of Food and Nutrition Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tissue variation of mitochondrial oxidative phosphorylation efficiency in cold-acclimated ducklings
    Autorzy:
    Salin, Karine
    Teulier, Loïc
    Rey, Benjamin
    Rouanet, Jean-Louis
    Voituron, Yann
    Duchamp, Claude
    Roussel, Damien
    Powiązania:
    https://bibliotekanauki.pl/articles/1040299.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    liver
    skeletal muscle
    thermogenesis
    mitochondrial oxidative phosphorylation
    proton conductance
    Opis:
    We investigated the oxidative phosphorylation efficiency of liver and gastrocnemius muscle mitochondria in thermoneutral and cold-acclimated ducklings. The yield of oxidative phosphorylation was lower in muscle than in liver mitochondria, a difference that was associated with a higher proton conductance in muscle mitochondria. Cold exposure did not affect oxidative phosphorylation efficiency or basal proton leak in mitochondria. We conclude that the basal proton conductance of mitochondria may regulate mitochondrial oxidative phosphorylation efficiency, but is not an important contributor to thermogenic processes in cold-acclimated ducklings.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 4; 409-412
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Different properties of four molecular forms of protein kinase CK2 from Saccharomyces cerevisiae
    Autorzy:
    Domańska, Katarzyna
    Zieliński, Rafał
    Kubiński, Konrad
    Sajnaga, Ewa
    Masłyk, Maciej
    Bretner, Maria
    Szyszka, Ryszard
    Powiązania:
    https://bibliotekanauki.pl/articles/1041353.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    isoenzyme specificity
    protein phosphorylation
    protein kinase CK2
    yeast
    Opis:
    CK2 is a pleiotropic constitutively active serine/threonine protein kinase composed of two catalytic α- and two regulatory β-subunits, whose regulation is still not well understood. It seems to play an essential role in regulation of many cellular processes. Four active forms of CK2, composed of αα'ββ', α2ββ', α'2ββ', and a free α'-subunit were isolated from wild-type yeast and strains containing a single deletion of the catalytic subunit. Each species exhibits properties typical for CK2, but they differ in substrate specificity and sensitivity to inhibitors. This suggests that each CK2 isomer may regulate different process or may differ in the way of its regulation.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 4; 947-951
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A new approach to phosphorylation of nucleosides using oxyonium phosphobetaines as intermediates
    Autorzy:
    Materna, Magdalena
    Stawinski, Jacek
    Sobkowski, Michał
    Powiązania:
    https://bibliotekanauki.pl/articles/16673995.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    synthesis
    phosphorylation
    N-oxides
    nucleotides
    oxyonium phosphobetaines
    H-phosphonates
    Opis:
    Oxyonium phosphobetaines are recently discovered molecules with a unique ¯O–P–O–N+ bond system, which makes them useful and versatile intermediates for the synthesis of phosphates and their derivatives. In this paper, the preliminary data on the application of these compounds in nucleoside phosphorylation were presented.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 1; 93-99
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prp4 kinase is required for proper segregation of chromosomes during meiosis in Schizosaccharomyces pombe
    Autorzy:
    Pozgajova, Miroslava
    Cipak, Lubos
    Trakovicka, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039511.pdf
    Data publikacji:
    2013
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Prp4 protein kinase
    S. pombe
    meiosis
    segregation
    protein phosphorylation
    Opis:
    Chromosome segregation during meiosis is a complex process, which leads to production of four haploid gametes from two precursor cells. Reversible phosphorylation of proteins plays a crucial role in this process. The Schizosaccharomyces pombe Prp4 is an essential serine/threonine protein kinase, which belongs to the Clk/Sty family. To study the role of Prp4 in meiosis, we analysed chromosome segregation in a strain carrying conditional analog-sensitive allele of Prp4 protein kinase (prp4-as2). Our data show, that Prp4 protein kinase plays important role in chromosome segregation during meiosis, as revealed by enhanced missegregation of chromosomes in prp4-as2 mutant cells.
    Źródło:
    Acta Biochimica Polonica; 2013, 60, 4; 871-873
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    High sequence homology between protein tyrosine acid phosphatase from boar seminal vesicles and human prostatic acid phosphatase
    Autorzy:
    Wysocki, Paweł
    Płucienniczak, Grażyna
    Strzeżek, Jerzy
    Powiązania:
    https://bibliotekanauki.pl/articles/1040544.pdf
    Data publikacji:
    2009
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    phosphorylation
    boar
    protein tyrosine phosphatase
    seminal plasma
    prostatic acid phosphatase
    Opis:
    Boar seminal vesicle protein tyrosine acid phosphatase (PTAP) and human prostatic acid phosphatase (PAP) show high affinity for protein phosphotyrosine residues. The physico-chemical and kinetic properties of the boar and human enzymes are different. The main objective of this study was to establish the nucleotide sequence of cDNA encoding boar PTAP and compare it with that of human PAP cDNA. Also, the amino-acid sequence of boar PTAP was compared with the sequence of human PAP. PTAP was isolated from boar seminal vesicle fluid and sequenced. cDNA to boar seminal vesicle RNA was synthesized, amplified by PCR, cloned in E. coli and sequenced. The obtained N-terminal amino-acid sequence of boar PTAP showed 92% identity with the N-terminal amino-acid sequence of human PAP. The determined sequence of a 354 bp nucleotide fragment (GenBank accession number: GQ184596) showed 90% identity with the corresponding sequence of human PAP. On the basis of this sequence a 118 amino acid fragment of boar PTAP was predicted. This fragment showed 89% identity with the corresponding fragment of human PAP and had a similar hydropathy profile. The compared sequences differ in terms of their isoelectric points and amino-acid composition. This may explain the differences in substrate specificity and inhibitor resistance of boar PTAP and human PAP.
    Źródło:
    Acta Biochimica Polonica; 2009, 56, 3; 481-486
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of vanadate on Pichia pastoris growth, protein kinase A activity and ribosomal protein phosphorylation.
    Autorzy:
    Frajnt, Magdalena
    Cytryńska, Małgorzata
    Jakubowicz, Teresa
    Powiązania:
    https://bibliotekanauki.pl/articles/1043701.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    protein phosphorylation
    vanadate
    Pichia pastoris
    PKA
    ribosomal proteins
    protein kinases
    Opis:
    It was found that wild type yeast Pichia pastoris can tolerate vanadate concentration as high as 25 mM in the growth medium. Moreover, four vanadate-resistant P. pastoris strains designated JC100/1, JC100/3, JC100/9 and JC100/15 exhibiting tolerance up to 150 mM vanadate were selected. Growth of P. pastoris was correlated with vanadate to vanadyl reduction and its accumulation in the growth medium. In two selected strains, JC100/9 and JC100/15, protein kinase A activity was much higher in comparison to the wild type strain even without vanadate addition to the growth medium. Moreover, in the presence of vanadate, protein kinase A activity was significantly increased in the wild type and the vanadate-resistant JC100/1 and JC100/3 strains. It was also found that phosphorylation of a 40 kDa protein associated with ribosomes occured in all vanadate-resistant strains from the logarithmic, while in the wild type strain from the stationary growth phase. From the presented results it can be concluded that a protein kinase A signalling pathway(s) might be involved in the mechanism of P. pastoris vanadate resistance. The results also indicate a possible role of the 40 kDa protein in protection of P. pastoris against vanadate toxicity.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 4; 959-968
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Altered mouse leukemia L1210 thymidylate synthase, associated with cell resistance to 5-fluoro-dUrd, is not mutated but rather reflects posttranslational modification
    Autorzy:
    Cieśla, Joanna
    Frączyk, Tomasz
    Zieliński, Zbigniew
    Sikora, Jacek
    Rode, Wojciech
    Powiązania:
    https://bibliotekanauki.pl/articles/1041288.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    protein phosphorylation
    L1210
    thymidylate synthase
    posttranslational modification
    FdUrd resistance
    Opis:
    Thymidylate synthase purified from 5-fluoro-dUrd-resistant mouse leukemia L1210 cells (TSr) was less sensitive to slow-binding inhibition by 5-fluoro-dUMP than the enzyme from the parental cells (TSp), both enzyme forms differing also in sensitivity to several other dump analogues, apparent molecular weights of monomer and dimer, and temperature dependence of the catalyzed reaction. Direct sequencing of products obtained from RT-PCR, performed on total RNA isolated from the parental and 5-fluoro-dUrd-resistant cells, proved both nucleotide sequences to be identical to the mouse thymidylate synthase coding sequence published earlier (NCBI protein database access no. NP_067263). This suggests that the altered properties of TSr are caused by a factor different than protein mutation, presumably posttranslational modification. As a possibility of rat thymidylate synthase phosphorylation has been recently demonstrated (Samsonoff et al. (1997) J Biol Chem 272: 13281), the mouse enzyme amino-acid sequence was analysed, revealing several potential phosphorylation sites. In order to test possible influence of the protein phosphorylation state on enzymatic properties, endogenous TSp and TSr were purified in the presence of inhibitors of phosphatases. Although both enzyme forms were phosphorylated, as shown by electrophoretical separation followed by phosphoprotein detection, the extent of phosphorylation was apparently similar. However, the same two purified enzyme preparations, compared to the corresponding preparations purified in the absence of phosphatase inhibitors, showed certain properties, including sensitivity to the slow-binding inhibition by FdUMP, altered. Thus properties dependence on phosphorylation was indicated.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 1; 189-198
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Short-term regulation of the mammalian pyruvate dehydrogenase complex
    Autorzy:
    Strumiło, Sławomir
    Powiązania:
    https://bibliotekanauki.pl/articles/1041314.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    pyruvate dehydrogenase complex
    thiamine diphosphate
    phosphorylation
    dephosphorylation
    regulation
    allosteric effect
    Opis:
    In this minireview the main mechanism of control of mammalian pyruvate dehydrogenase complex (PDHC) activity by phosphorylation-dephosphorylation is presented in the first place. The information recently obtained in several laboratories includes new data about isoforms of the PDH converting enzymes (kinase and phosphatase) and their action in view of short-term regulation of PDHC. Moreover, interesting influence of exogenous thiamine diphosphate (TDP) and some divalent cations, especially Mn2+, on the kinetic parameters of PDHC saturated with endogenous tightly bound TDP, is discussed. This influence causes a shortening of the lag-phase of the catalyzed reaction and a strong decrease of the Km value of PDHC mainly for pyruvate. There are weighty arguments that the effects have an allosteric nature. Thus, besides reversible phosphorylation, also direct manifold increase of mammalian PDHC affinity for the substrate by cofactors seems an important aspect of its regulation.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 4; 759-764
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dinitrofenol – mieszanina izomerów. Dokumentacja proponowanych wartości dopuszczalnych wielkości narażenia zawodowego
    Dinitrophenol
    Autorzy:
    Gralewicz, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/137927.pdf
    Data publikacji:
    2005
    Wydawca:
    Centralny Instytut Ochrony Pracy
    Tematy:
    dinitrofenol
    fosforylacja oksydatywna
    metabolizm
    zaćma
    dinitrophenol
    oxidative phosphorylation
    metabolism
    cataract
    Opis:
    Dinitrofenol (DNP) – mieszanina izomerów: 2,3- DNP, 2,4- DNP i 2,6-DNP z przewagą 2,4-DNP, jest stosowany w produkcji barwników, kwasu pikrynowego i pikraminowego, wywoływaczy fotograficznych i materiałów wybuchowych oraz jako pestycyd w rolnictwie i sadownictwie. Dinitrofenol jest trucizną metaboliczną, a mechanizm jego działania toksycznego polega na rozprzęganiu fosforylacji oksydatywnej. Zawodowe narażenie na pary i pyły 2,4-DNP może wywoływać objawy wzmożonego metabolizmu. 2,4-DNP nie jest kancerogenem, nie wykazuje także działania genotoksycznego ani mutagennego. Za podstawę wyliczenia wartości NDS dla 2,4-DNP przyjęto wartość LOAEL dla skutków metabolicznych. Wartość ta u człowieka wynosi 1,2 mg/kg/dzień. W warunkach narażenia drogą oddechową taką dawkę pracownik może wchłonąć, gdy stężenie 2,4-DNP we wdychanym powietrzu wynosi 10,5 mg/m3. Przyjmując współczynnik niepewności równy 16 (2 dla różnic we wrażliwości osobniczej, 2 dla przejścia z wartości LOAEL do wartości NOAEL, 2 dla różnicy w sposobie narażenia i 2 dla ekstrapolacji z narażenia średnioterminowego do przewlekłego), to wyliczona wartość normatywu będzie wynosiła 0,66 mg/m3. W związku z powyższym proponuje się przyjęcie stężenia 0,5 mg/m3 za wartość NDS dla 2,4-DNP. Przyjmując, że 2,4-DNP jest najbardziej toksycznym izomerem dinitrofenolu oraz, że udział tego izomeru w dinitrofenolu – mieszaninie izomerów jest dominujący, proponujemy przyjęcie dla dinitrofenolu – mieszaniny również takiej samej wartości NDS jaką zaproponowano dla 2,4-DNP.
    Dinitrophenol (DNP) is a mixture of 2,4-DNP and smaller amounts of 2,3-DNP and 2,6-DNP). It is a yellow, crystalline solid. DNP is used in synthesis of dyes, picric acid, picramic acid, wood preservatives, photographic developers, explosives, and insecticides. In the 1930s, 2,4-DNP was used as a weight-reducing drug. Short-term exposure to DNP may affect metabolism resulting in hyperthermia. High-level exposure may be fatal. The existing data concerning the health effects of 2,4-DNP oral exposure in humans indicate that the characteristic effects of 2,4-DNP for this route are: increased basal metabolic rate and perspiration, weight loss, a sensation of warmth, and – at higher dosage – increased heart and respiratory rate, and increased body temperature. Repeated or prolonged contact with the skin may cause dermatitis. Exposure to DNP may result in changes in the functional state of the peripheral nervous system, cardiovascular system and gastrointestinal system. It may also induce cataracts. Taking into account the results obtained in clinical studies on people ingesting 2,4-DNP (LOAEL for metabolic effects was 1.2 mg/kg/day), a concentration 0.5 mg of dinitrophenol/m3 is proposed as a maximum exposure limit (maximum admissible concentration) with a skin notation. With regard to systemic effects of DNP no STEL value has beeen established.
    Źródło:
    Podstawy i Metody Oceny Środowiska Pracy; 2005, 1 (43); 67-89
    1231-868X
    Pojawia się w:
    Podstawy i Metody Oceny Środowiska Pracy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Phosphorylation of basic amino acid residues in proteins: important but easily missed
    Autorzy:
    Cieśla, Joanna
    Frączyk, Tomasz
    Rode, Wojciech
    Powiązania:
    https://bibliotekanauki.pl/articles/1039902.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    basic amino acids
    posttranslational modification
    phosphorylation
    acid-labile
    base-stable
    phosphoramidate
    Opis:
    Reversible phosphorylation is the most widespread posttranslational protein modification, playing regulatory role in almost every aspect of cell life. The majority of protein phosphorylation research has been focused on serine, threonine and tyrosine that form acid-stable phosphomonoesters. However, protein histidine, arginine and lysine residues also may undergo phosphorylation to yield acid-labile phosphoramidates, most often remaining undetected in conventional studies of protein phosphorylation. It has become increasingly evident that acid-labile protein phosphorylations play important roles in signal transduction and other regulatory processes. Beside acting as high-energy intermediates in the transfer of the phosphoryl group from donor to acceptor molecules, phosphohistidines have been found so far in histone H4, heterotrimeric G proteins, ion channel KCa3.1, annexin 1, P-selectin and myelin basic protein, as well as in recombinant thymidylate synthase expressed in bacterial cells. Phosphoarginines occur in histone H3, myelin basic protein and capsidic protein VP12 of granulosis virus, whereas phospholysine in histone H1. This overview of the current knowledge on phosphorylation of protein basic amino-acid residues takes into consideration its proved or possible roles in cell functioning. Specific requirements of studies on acid-labile protein phosphorylation are also indicated.
    Źródło:
    Acta Biochimica Polonica; 2011, 58, 2; 137-148
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Phosphorylation sites of HER2/c-erbB-2: role in cell growth and in disease
    Autorzy:
    Khurshid, Rukhshan
    Saleem, Mahjabeen
    Gul-e-Raana, -
    Akhthar, Muhammad
    Powiązania:
    https://bibliotekanauki.pl/articles/1039198.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    phosphorylation sites
    HER2/c-erbB-2
    cell growth and disease
    Opis:
    The protein kinase c-erbB-2 belongs to the family of receptor tyrosine kinase and is involved in oncogenesis. The present study predicts different phosphorylation sites of HER2/c-erbB-2 which are important in preventing or developing cancer, especially breast cancer. Sequence homology showed highest homology (77%) with epidermal growth factor receptor kinase domain. According to PROSITE search result, active sites of c-erbB-2 are N-lobe (glycine rich phosphate binding loop). Catalytic loop with presumptive catalytically active of Asp108 is phosphorylated by tyrosine protein kinase. A-loop, activation loop, becomes phosphorylated and activates the substrate binding. The study strengthens our knowledge regarding HER2 signaling by the detection of uncharacterized signaling proteins, establishing phosphorylation of an activation loop and helps us to make assumptions about the role of such previously unidentified proteins. On the basis of importance of HER2 in breast cancer as well as in other diseases, this study provides fruitful information for designing new therapeutic strategies.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 4; 699-703
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Human mitochondria in health, disease, ageing and cancer
    Autorzy:
    Bartnik, E
    Lorenc, A.
    Mroczek, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2041927.pdf
    Data publikacji:
    2001
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    health
    oxidative phosphorylation
    disease
    genetics
    mitochondrion
    man
    human cell
    aging
    metabolic process
    cancer
    Źródło:
    Journal of Applied Genetics; 2001, 42, 1; 65-71
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    IAA and BAP affect protein phosphorylation-dependent processes during sucrose-mediated G1 to S and G2 to M transitions in root meristem cells of Vicia faba
    Autorzy:
    Polit, J T
    Maszewski, J.
    Rosiak, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/58127.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    Vicia faba
    cell cycle
    sucrose
    auxin
    protein phosphorylation
    cytokinin
    control point
    meristem
    root
    Opis:
    In carbohydrate-starved root meristems of Vicia faba subsp. minor, the expression of two Principal Control Points located at the final stages of the G1 (PCP1) and G2 (PCP2) phases has been found to be correlated with a marked decrease of protein phosphorylation within cell nuclei, nucleoli and cytoplasm. Adopting the same experimental model in our present studies, monoclonal FITC conjugated antibodies that recognize phosphorylated form of threonine (αTPab-FITC) were used to obtain an insight about how the indole-3-acetic acid (IAA), benzyl-6-aminopurine (BAP), and the mixture of both phytohormones influence the time-course changes in an overall protein phosphorylation during sucrose-mediated PCP1→S and PCP2→M transitions. Unsuspectedly, neither IAA, BAP, nor the mixture of both phytohormones supplied in combination with sucrose did up-regulate protein phosphorylation. However using the block-and-release method, it was shown that root meristems of Vicia provided with sucrose alone indicated higher levels of αTPab-FITC. Contrarily, phytohormones supplied in combination with sucrose induced apparent decline in phosphorylation of cell proteins, which - when compared with the influence of sucrose alone - became increasingly evident in time. Thus, it seems probable, that a general decline in the amount of αTPab-FITC labeled epitopes may overlay specific phosphorylations and dephosphorylations governed by the main cell cycle kinases and phosphatases.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2004, 73, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Guard cells anion channel SLAC1 is activated by a set of kinases originating from three different families
    Autorzy:
    Diekmann, M.
    Geiger, D.
    Hedrich, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/81003.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    drought stress
    plant
    guard cell
    anion channel
    abscisic acid
    protein kinase
    phosphorylation
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Protein phosphatase 2A: Variety of forms and diversity of functions
    Autorzy:
    Lechward, Katarzyna
    Awotunde, Olubunmi
    Świątek, Wojciech
    Muszyńska, Grażyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1044037.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    protein phosphatase 2A
    signal transduction
    protein-protein interactions
    reversible phosphorylation
    cell cycle
    carcinogenesis.
    Opis:
    Protein phosphatase 2A (PP2A) comprises a diverse family of phosphoserine-and phosphothreonine-specific phosphatases present in all eukaryotic cells. All forms of PP2A contain a catalytic subunit (PP2Ac) which forms a stable complex with the structural subunit PR65/A. The heterodimer PP2Ac-PR65/A associates with regulatory proteins, termed variable subunits, in order to form trimeric holoenzymes attributed with distinct substrate specificity and targeted to different subcellular compartments. PP2Ac activity can be modulated by reversible phosphorylation on Tyr307 and methylation on C-terminal Leu309. Studies on PP2A have shown that this enzyme may be implicated in the regulation of metabolism, transcription, RNA splicing, translation, differentiation, cell cycle, oncogenic transformation and signal transduction.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 4; 921-933
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Functional and biochemical characterization of Arabidopsis calcium sensor SCS a potential regulator of SnRK2 protein kinases
    Autorzy:
    Bucholc, M.
    Goch, G.
    Ciesielski, A.
    Anielska-Mazur, A.
    Dobrowolska, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/80164.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    biochemical characteristics
    functional characteristics
    Arabidopsis
    protein kinase
    water deficit
    calcium binding protein
    phosphorylation
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nuclear localization and binding affinity of STAT5b for the α2-macroglobulin gene promoter during rat liver development and the acute-phase response
    Autorzy:
    Mihailović, Mirjana
    Dinić, Svetlana
    Bogojević, Desanka
    Ivanović-Matić, Svetlana
    Uskoković, Aleksandra
    Arambašić, Jelena
    Grigorov, Ilijana
    Grdović, Nevena
    Vidaković, Melita
    Martinović, Vesna
    Petrović, Miodrag
    Poznanović, Goran
    Powiązania:
    https://bibliotekanauki.pl/articles/1041082.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    rat liver development
    phosphorylation
    nuclear extract
    nuclear matrix
    STAT5b
    α2-macroglobulin
    Opis:
    Expression of the rat α2-macroglobulin (MG) gene undergoes dynamic changes throughout an individual's life and during the acute-phase (AP) response. Details of the participation of the STAT family of transcription factors in its control remain incompletely understood. Here we examined the involvement of STAT5b in MG gene expression during development and the AP response. Immuno-blot analysis revealed the highest nuclear level of STAT5b in the fetus and during postnatal development, whereas in the adult it decreased. Stimulation of MG expression during the AP response was accompanied by a decrease in STAT5b. Examination of STAT5b localization revealed that the relative concentrations of STAT5b were higher in the nuclear matrix than in the nuclear extract. Affinity chromatography with the extended promoter region of the MG gene (-825/+12), followed by immuno-blot analysis, revealed dynamic changes in STAT5b binding. The highest concentration of the promoter-binding form of STAT5b was observed in the fetus. As postnatal development progressed, the level of promoter-bound STAT5b decreased and in the adult liver it was the lowest. Stimulation of MG gene expression during the AP response in both the fetus and adult was accompanied by significantly decreased STAT5b binding to the MG promoter. The AP response was accompanied by lower levels of STAT5b serine and tyrosine phosphorylation in both fetus and adult. In the nuclear matrix derived from adult tissues, tyrosine phosphorylated species were completely absent. We conclude that developmental-stage differences in the mechanisms that determine STAT5b nuclear localization contribute to its activity in vivo.
    Źródło:
    Acta Biochimica Polonica; 2007, 54, 2; 331-340
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of phosphorylation and succinilation of yeast protein on its enzymatic digestibility and functional properties
    Wpływ fosforylacji i sulecynylacji białka drożdżowego na jego strawność enzymatyczną i właściwości funkcjonalne
    Autorzy:
    Giec, A.
    Stasińska, B.
    Lampart-Szczapa, E.
    Skupin, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/1399535.pdf
    Data publikacji:
    1989
    Wydawca:
    Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
    Tematy:
    protein of the yeast Torula utilis
    protein phosphorylation
    protein succinilation
    functional properties
    enzymatic digestibility
    Opis:
    It was found that succinilation and phosphorylation of protein lead to its greater yield from yeast compared to the traditional alkaline extraction, causing a more effective reduction of nucleic acids content in the final product and a considerable improvement of the functional properties of isolated substances. At the same time phosphorylation caused a smaller reduction of enzymatic digestibility of the protein accompanying all the advantageous effects.
    Włączenie białka mikrobiologicznego do żywności wymaga dostosowania jego właściwości fizykochemicznych. W literaturze opisano wiele metod osiągnięcia tego celu dzięki różnym modyfikacją chemicznym. Brak jest analizy porównawczej ich skuteczności oraz wpływu na wartość biologiczną uzyskanych preparatów białkowych. Celem niniejszej pracy było porównanie efektywności zabiegów sulecynylacji i fosforylacji białka drożdżowego z tradycyjną estrakcją alkaliczną po hydrolizie kwa sów nukleinowych. Badania wykazały że proces sulecynylacji oraz fosforylacji zwiększa ekstraktywność białka wskutek przesunięcia punktu izoelektrycznego, co wpływa istotnie na obniżenie zawartości kwasów nukleinowych w produkcie o ok. 60 % i poprawę właściwości funkcjonalnych (tabela). Fosforylacja w znacznie mniejszym topniu obniżyła strawność enzymatyczną preparatu niż sulecynylacja, a dodatkowo z uwagi na swą odwracalność wydaje się być godną polecenia metodą uzyskiwania z homogenatów drożdżowych białka przeznaczonego do żywności.
    Źródło:
    Acta Alimentaria Polonica; 1989, 15(39), 1; 63-66
    0137-1495
    Pojawia się w:
    Acta Alimentaria Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Role of spruce respiratory burst oxidase homolog (RBOH) during lignin formation in Norway spruce
    Autorzy:
    Nickolov, K.
    Laitinen, T.
    Haggman, H.
    Teeri, T.
    Karkonen, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/80939.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    Norway spruce
    respiratory burst
    lignin formation
    hydrogen peroxide
    NADPH oxidase
    phosphorylation
    protein-protein interaction
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. I. The technology for the preparation of the primers
    Autorzy:
    Borowicz, B P
    Powiązania:
    https://bibliotekanauki.pl/articles/65836.pdf
    Data publikacji:
    1997
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    phosphorylation
    plant protection
    I-18 C gene
    purification
    Chironomus tentans
    polymerase chain reaction
    DNA fragment
    oligonucleotide
    Opis:
    The above mentioned technology in a case of the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene is described i.e. the primers design, purification and phosphorylation of the oligonucleotide primers.
    Dla określonego w tytule pracy, celu badań przedstawiono technologię przygotowywania starterów, w przypadku DNA odpowiadającego Otwartej Ramie Odczytu II genu I-18 C Chironomus tentans. Technologia ta obejmowała zaprojektowanie starterów dla PCR oraz oczyszczenie i fosforylację zsyntetyzowanych oligonukleotydowych starterów.
    Źródło:
    Journal of Plant Protection Research; 1997, 37, 1-2
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    PP2A controls methionine metabolism elicited by intracellular H2O2
    Autorzy:
    Trotta, A.
    Li, S.
    Mhamdi, A.
    Ravanel, S.
    Noctor, G.
    Kangasjarvi, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/80912.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    intracellular signalling
    plant cell
    reactive oxygen species
    protein
    post-translational modification
    phosphorylation
    methionine
    hydrogen peroxide
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    SDS/PAGE characteristics of protein kinases tightly associated with chick embryo brain ribosomes
    Autorzy:
    Sanecka-Obacz, Maria
    Powiązania:
    https://bibliotekanauki.pl/articles/1044449.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    protein phosphorylation
    chick brain development
    ribosomal kinases
    CK2
    PKA
    CK1
    fetal brain
    brain ribosomes
    Opis:
    Protein kinases tightly associated with chick embryo brain ribosomes washed with Triton X-100 and KCl were characterized by their ability to phosphorylate ribosomes and two exogenous substrates, histone IIA and casein. c-AMP-dependent kinase (PKA) and casein kinases (CK1, CK2) were examined in the presence of specific modulators by SDS/ PAGE followed by  renaturation in gel assay according to Kameshita & Fujisawa (Anal. Biochem. 1989, 183, 139-143). Basing on these data it can be presumed that PKA activity increases, but the levels of CK2 and CK1 decrease during chick embryo development.
    Źródło:
    Acta Biochimica Polonica; 1999, 46, 4; 911-917
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Modyfikacje epigenetyczne jako potencjalne cele terapii antynowotworowych
    Epigenetic modifications as potential targets of anti-cancer therapy
    Autorzy:
    Kulczycka, Anna
    Bednarek, Ilona
    Dzierżewicz, Zofia
    Powiązania:
    https://bibliotekanauki.pl/articles/1034844.pdf
    Data publikacji:
    2013
    Wydawca:
    Śląski Uniwersytet Medyczny w Katowicach
    Tematy:
    epigenetyka
    dna
    histony
    metylacja
    acetylacja
    fosforylacja
    terapie antynowotworowe
    epigenetics
    histones
    methylation
    acetylation
    phosphorylation
    anti-cancer treatments
    Opis:
    Epigenetics analyses inherited characteristics not directly connected to the DNA nucleotide sequence. It investigates the relationships between biochemical modifications and the expression of selected genes. Initially, it was thought that gene expression depends on information encoded in the DNA sequence. However, it was discovered that the activity of many enzymes like methylases, demethylases, acetylases, deacetylases is necessary to regulate this process and its dysregulations may lead to e.g. cancer initiation and progression. Epigenetics has an impact on neoplastic transformation by reducing the global level of DNA methylation and increasing the methylation level within tumour suppressor gene promoters, which significantly impairs the repression of carcinogenesis. Additionally, modifications of histone proteins, based on disorders of acetylation-deacetylation and methylation-demethylation processes, may lead to overexpression of genes involved in cancer development. Numerous examples have been described, among others breast, prostate and colon cancers, depending on the modification of histone amino tails, primarily of histone H3. For such reasons, the possibility of using many therapies which can reverse the negative effect of these modifications by e.g. DNA demethylation (DNA demethylating drugs) or re-acetylation of histone lysine resides (histone deacetylase inhibitors) is examined. In the near future, epigenetics probably will allow the effective treatment of some cancer diseases, although further research on the impact of enzymatic modifications on the development of carcinogenesis is still needed.
    Epigenetyka zajmuje się badaniem cech dziedzicznych, które nie zależą bezpośrednio od sekwencji nukleotydowej w DNA, ale są rezultatem modyfikacji biochemicznych na ekspresję wybranych genów. Początkowo uważano, że ekspresja genów zależy tylko od informacji zapisanej zawartej w sekwencji DNA, z czasem okazało się, że liczne modyfikacje będące rezultatem działania różnych grup enzymów, w tym metylaz, demetylaz, acetylaz czy deacetylaz, wpływają na regulację tego procesu, a zaburzenia regulacji aktywności tych enzymów mogą prowadzić do wystąpienia i rozwoju m.in. nowotworów. Epigenetyczny aspekt rozwoju transformacji nowotworowej wskazuje na obniżenie globalnego poziomu metylacji DNA oraz podwyższenie poziomu metylacji w obrębie promotorów genów supresorowych, co znacząco upośledza represję nowotworzenia. Dodatkowo, modyfikacje białek histonowych, opierające się na dysregulacji procesów acetylacji–deacetylacji i metylacji – demetylacji, prowadzą do nadekspresji genów zaangażowanych w rozwój kancerogenezy. Opisane zostały liczne przykłady zależności wystąpienia nowotworów, m.in. raka sutka, stercza czy okrężnicy od wystąpienia danej modyfikacji reszt aminokwasowych białek histonowych, w tym głównie histonu H3. Z takich też przyczyn podejmowane są próby zastosowania terapii odwracających negatywny skutek wybranych modyfikacji, np. poprzez demetylację DNA (leki demetylujące DNA) czy reacetylację reszt lizynowych histonów (inhibitory decetylaz histonów). W niedalekiej przyszłości epigenetyka najprawdopodobniej u możliwi skuteczne leczenie części chorób nowotworowych, aczkolwiek konieczne są dalsze badania wpływu modyfikacji enzymatycznych na mechanizm rozwoju kancerogenezy.
    Źródło:
    Annales Academiae Medicae Silesiensis; 2013, 67, 3; 201-208
    1734-025X
    Pojawia się w:
    Annales Academiae Medicae Silesiensis
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Nucleotide analogue, method of synthesis of nucleotide analogue, use of nucleotide analogue, antiviral pro-nucleotide, pharmaceutical composition
    Autorzy:
    Kraszewski, A.
    Romanowska, J.
    Szymanska-Michalak, A.
    Sobkowski, M.
    Boryski, J.
    Stawinski, J.
    Lipniacki, A.
    Piasek, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/81225.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    nucleotide analogue
    phosphorylation
    HIV
    viral disease
    physicochemical property
    pharmacokinetic parameter
    anti-HIV activity
    antiviral pro-nucleotide
    patent
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2014, 95, 4
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Catalytic activity of mutants of yeast protein kinase CK2α
    Autorzy:
    Sajnaga, Ewa
    Kubiński, Konrad
    Szyszka, Ryszard
    Powiązania:
    https://bibliotekanauki.pl/articles/1040685.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    protein phosphorylation
    ATP binding
    mutagenesis
    protein kinase CK2
    ATP-competitive inhibitors
    Saccharomyces cerevisiae
    spermine
    heparin
    yeast
    Opis:
    Yeast CK2 is a highly conserved member of the protein kinase CGMC subfamily composed of two catalytic (α and α') and two regulatory (β and β') subunits. The amino-acid sequences of both catalytic subunits are only 60% homologous. Modelling of the tertiary structure of the CK2α displays additional α-helical structures not present in the CK2α' subunit, connecting the ATP-binding loop with the catalytic and activation loops. Deletion of this part causes drastic structural and enzymatic changes of the protein (CK2αΔ91-128) with characteristics similar to yeast CK2α' (low sensitivity to salt, heparin and spermine). Additionally, the deletion causes an over 5-fold decrease of the binding affinity for ATP and ATP-competitive inhibitors (TBBt and TBBz). The structural basis for TBBt and TBBz selectivity is provided by the hydrophobic pocket adjacent to the ATP/GTP binding site, which is smaller in CK2 than in the majority of other protein kinases. The importance of hydrophobic interactions in the binding of specific inhibitors was investigated here by mutational analysis of CK2α residues whose side chains contribute to reducing the size of the hydrophobic pocket. Site-directed mutagenesis was used to replace Val67 and Ile213 by Ala. The kinetic properties of the single mutants CK2αVal67Ala and CK2αIle213Ala, and the double mutant CK2Val67Ala Ile213Ala were studied with respect to ATP, and both inhibitors TBBt and TBBz. The Km values for ATP did not change or were very close to those of the parental kinase. In contrast, all CK2α mutants analysed displayed higher Ki values towards the inhibitors (10 to 12-fold higher with TBBt and 3 to 6-fold with TBBt) comparing to recombinant wild-type CK2α.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 4; 767-776
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    "MitoTea": Geranium robertianum L. decoctions decrease blood glucose levels and improve liver mitochondrial oxidative phosphorylation in diabetic Goto-Kakizaki rats
    Autorzy:
    Ferreira, Fernanda
    Peixoto, Francisco
    Nunes, Elsa
    Sena, Cristina
    Seiça, Raquel
    Santos, Maria
    Powiązania:
    https://bibliotekanauki.pl/articles/1040293.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    oxidative phosphorylation
    herbal medicine
    Vaccinium myrtillus L.
    Goto-Kakizaki (GK) rats
    type 2 diabetes mellitus
    Geranium robertianum L.
    Opis:
    Several chemical compounds found in plant products have proven to possess beneficial properties, being currently pointed out due to their pharmacological potential in type 2 diabetes mellitus complications. In this context, we studied the effect of Geranium robertianum L. (herb Robert) leaf decoctions in Goto-Kakizaki (GK) rats, a model of type 2 diabetes. Our results showed that oral administration of G. robertianum leaf decoctions over a period of four weeks lowered the plasma glucose levels in diabetic rats. Furthermore, the treatment with G. robertianum extracts improved liver mitochondrial respiratory parameters (state 3, state 4 and FCCP-stimulated respiration) and increased oxidative phosphorylation efficiency.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 4; 399-402
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Binding of Aedes aegypti trypsin modulating oostatic factor [Aea-TMOF] to its receptor stimulates phosphorylation and protease processing of gut-membrane proteins
    Autorzy:
    Borovsky, D.
    Hamdaoui, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/55079.pdf
    Data publikacji:
    2008
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    protease processing
    Aedes aegypti
    electrophoresis
    trypsin modulating oostatic factor
    phosphorylation
    mosquito
    insect
    larva
    gut-membrane protein
    fluorography
    gut receptor
    Opis:
    The binding of TMOF to its gut receptor was followed by incubating guts removed from male and female Aedes aegypti. TMOF at physiological concentrations, in the presence of [γ32P]ATP, causes phosphorylation and release of gut-membrane protein (45 kDa) that is further processed by proteolysis. In the presence of protease inhibitors only the 45 kDa protein was released. The phosphorylation and processing of the 45 kDa protein does not happen in the absence of TMOF. Both larvae and adult guts release the protein in the presence of TMOF. Male Ae. aegypti do not synthesize trypsin in their gut and do not release the 45 kDa protein in the presence of TMOF because a TMOF receptor is probably absent. Homogenized guts do not release the 45 kDa protein, indicating that the protease processing or the ecto-protein kinase activity is probably reduced after breaking the tissue. The 45 kDa phosphorylated protein can be dephosphorylated by alkaline phosphatase and protein phosphatase, indicating that the phosphate group is covalently linked to either a serine or a tyrosine moiety. This is the first report that shows that in insects, binding of a peptide hormone activates its receptor by proteolysis.
    Źródło:
    Pestycydy; 2008, 1-2; 13-25
    0208-8703
    Pojawia się w:
    Pestycydy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Direct, calcium-independent, regulation of NADPH oxidase during plant innate immunity
    Autorzy:
    Kodota, Y.
    Sklenar, J.
    Derbyshire, P.
    Stransfeli, L.
    Asai, S.
    Ntoukakis, V.
    Shirasu, K.
    Jones, A.
    Zipfel, C.
    Powiązania:
    https://bibliotekanauki.pl/articles/80067.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    plant
    innate immunity
    pathogen-associated molecular pattern
    pattern recognition receptor
    reactive oxygen species
    NADPH oxidase
    calcium-dependent protein kinase
    phosphorylation
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Spektrometria mas w analizie białek i peptydów : znaczniki jonizacyjne
    Mass spectrometry in analysis of peptides and proteins : ionization markers
    Autorzy:
    Bąchor, R.
    Biernat, M.
    Cebrat, M.
    Kijewska, M.
    Kluczyk, A.
    Kuczer, M.
    Paluch, A.
    Waliczek, M.
    Wierzbicka, M.
    Stefanowicz, P.
    Szewczuk, Z.
    Powiązania:
    https://bibliotekanauki.pl/articles/171509.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Chemiczne
    Tematy:
    spektrometria mas
    modyfikacje potranslacyjne
    znaczniki jonizacyjne
    fosforylacja
    glikacja
    biblioteki kombinatoryczne
    mass spectrometry
    post-translational modifications
    ionization markers
    phosphorylation
    glycation
    combinatorial libraries
    Opis:
    High sensitivity, accuracy, and ability to provide structural information makes mass spectrometry (MS) the method of choice for both qualitative and quantitative analysis in proteome research. Peptide sequencing by tandem mass spectrometry (MS/MS) was successfully applied to discover new peptide sequences and modifications. Insufficient ionization of some peptides is one of the main limitations of MS- based peptide identification. The development of sensitive detection techniques for the efficient analysis of such samples is very important. Differences in ionizability cause difficulties in quantification studies, which could be overcome by derivatization of peptides to improve both the detectability and the selectivity of an analysis. Incorporation of ionization markers and isotopic labels (particularly the isobaric tags) is often used for this reason. Isobaric labeling reagents (including commercially available iTRAQ, TMT, DiLeu and DiART) have found a wide application in quantitative proteomics. Mass spectrometry is a very good tool for the determination of posttranslational modifications (PTMs), but the modified proteins are usually present in low concentrations. The development of ionization tags specific to a particular PTM and suitable for sensitive analysis of the modified proteins is required. For the analysis of phosphorylated peptides, a combination of β-elimination and the reaction of resulting α,β-dehydroamino acid residues with the nucleophilic thiol group could be used to detect a labile PTM. Such reaction may be used to introduce derivatizing reagents at the original site of phosphorylation, to enhance ionization in MS analysis. Glycation and glycosylation of proteins are other very important PTMs associated with many natural processes as well as diseases. We have designed and synthesized bifunctional quaternary ammonium salt derivatives of phenylboronic acids for selective detection of carbohydrates and peptide-derived Amadori products by ESI-MS. The attachment of a fixed charge (e.g. in a form of a quaternary ammonium salt) to the amino groups in peptides leads to the enhancement of a precursor ion signal in mass spectra. We have developed several new QAS-containing ionization reagents including bicyclic tags with DABCO, ABCO or azoniaspiro groups. It is worth noting that 2,4,6-substituted pyrylium salts react with amino groups in peptides introducing a stable positive charge and improve peptide detection by MS. The newly developed ionization tags were successfully applied for the analysis of OBOC combinatorial libraries as well as for studying possible biomarkers of preeclampsia, a pregnancy disorder.
    Źródło:
    Wiadomości Chemiczne; 2018, 72, 7-8; 609-633
    0043-5104
    2300-0295
    Pojawia się w:
    Wiadomości Chemiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A calcium-dependent protein kinase-NADPH oxidase activation circuit in rapid defense signal propagation
    Autorzy:
    Seybold, H.
    Dubiella, U.
    Lassing, R.
    Schulze, W.
    Romeis, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/80432.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    calcium-dependent protein kinase
    phosphorylation
    NADPH oxidase
    pathogen-associated molecular pattern
    innate immune system
    plant
    phytohormone
    gene expression
    reactive oxygen species
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Advantage of a baculovirus expression system for protein-protein interaction studies. Involvement of posttranslational phosphorylation in the interaction between wt p53 protein and poly(ADP-ribose) polymerase-1
    Autorzy:
    Schmid, Gerald
    Wojciechowski, Jacek
    Węsierska-Gądek, Józefa
    Powiązania:
    https://bibliotekanauki.pl/articles/1041382.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    nucleocytoplasmic shuttling
    NES
    p53 isomers
    p53 stability
    cell cycle arrest
    2D-PAGE
    p53 nuclear export
    FACS analysis
    p53 pull-down assay
    p53 phosphorylation
    Opis:
    We recently observed an interaction between poly(ADP-ribose) polymerase-1 (PARP-1) and the tumor suppressor p53 protein. However, more extensive studies on both proteins, especially those on characterization of their domains involved in the interaction were difficult due to very low expression levels of p53 in mammalian cells. Therefore, we generated recombinant proteins for such studies. To clarify which domains of human PARP-1 and of human wild-type (wt) p53 were involved in this protein-protein interaction, we generated baculoviral constructs encoding full length or distinct functional domains of both proteins. Full length PARP-1 was simultaneously coexpressed in insect cells with full length wt p53 protein or its distinct truncated fragments and vice versa. Reciprocal immunoprecipitation of Sf9 cell lysates revealed that the central and carboxy-terminal fragments of p53 each were sufficient to confer binding to PARP-1, whereas the amino-terminal part harbouring the transactivation functional domain was dispensable. On the other hand, the amino-terminal and central fragments of PARP-1 were both necessary for complex formation with p53 protein. Since the most important features of p53 protein are regulated by phosphorylation, we addressed the question whether its phosphorylation is essential for the binding between the two proteins. Baculovirally expressed wt p53 was post-translationally modified. At least six distinct p53 isomers were resolved by immunoblotting following two-dimensional separation of baculovirally expressed wt p53 protein. Using specific phospho-serine antibodies, we identified phosphorylation of baculovirally expressed p53 protein at five distinct sites. To define the role of p53 phosphorylation, pull-down assays using untreated and dephosphorylated p53 protein were performed. Dephosphorylated p53 failed to bind PARP-1, indicating that complex formation between the two proteins was regulated by phosphorylation of p53. The marked phosphorylation of p53 at Ser392 observed in unstressed cells suggests that the phosphorylated carboxy-terminal part of p53 undergoes complex formation with PARP-1 resulting in masking of the NES and thereby preventing its export.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 3; 713-719
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-43 z 43

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