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Wyświetlanie 1-15 z 92
Tytuł:
The 35 kDa acid metallophosphatase of the frog Rana esculenta liver: studies on its cellular localization and protein phosphatase activity.
Autorzy:
Szalewicz, Agata
Strzelczyk, Barbara
Sopel, Mirosław
Kubicz, Aleksandra
Powiązania:
https://bibliotekanauki.pl/articles/1043638.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
metallophosphatase
acid phosphatase
frog liver
protein phosphatase
tyrosine protein phosphatase
Opis:
The cellular localization of the 35 kDa, low molecular mass acid metallophosphatase (LMW AcPase) from the frog (Rana esculenta) liver and its activity towards P-Ser and P-Tyr phosphorylated peptides were studied. This enzyme was localized to the cytoplasm of hepatocytes but did not appear in other cells of liver tissue (endothelium, macrophages, blood cells). This LMW AcPase does not display activity towards 32P-phosphorylase a under conditions standard for the enzymes of PPP family. Proteins containing P-Ser: rabbit 32P-phosphorylase a and phosvitin are hydrolysed only at acidic pH and are poor substrates for this enzyme. The frog AcPase is not inhibited by okadaic acid and F- ions, the Ser/Thr protein phosphatase inhibitors. Moreover, the frog enzyme does not cross-react with specific antisera directed against N-terminal fragment of human PP2A and C-terminal conserved fragment of the eukaryotic PP2A catalytic subunits. These results exclude LMW AcPase from belonging to Ser/Thr protein phosphatases: PP1c or PP2Ac. In addition to P-Tyr, this enzyme hydrolyses efficiently at acidic pH P-Tyr phosphorylated peptides (hirudin and gastrin fragments). Km value for the hirudin fragment (7.55 ± 1.59 × 10-6 M) is 2-3 orders of magnitude lower in comparison with other substrates tested. The enzyme is inhibited competitively by typical inhibitors of protein tyrosine phosphatases (PTPases): sodium orthovanadate, molybdate and tungstate. These results may suggest that the LMW AcPase of frog liver can act as PTPase in vivo. A different cellular localization and different response to inhibition by tetrahedral oxyanions (molybdate, vanadate and tungstate) provide further evidence that LMW AcPase of frog liver is distinct from the mammalian tartrate-resistant acid phosphatases.
Źródło:
Acta Biochimica Polonica; 2003, 50, 2; 555-566
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of soil phosphatase activity, phosphorus and heavy metals content depending on the mineral fertilization
Ocena aktywności fosfatazy, zawartości fosforu i metali ciężkich w zależności od nawożenia mineralnego
Autorzy:
Lemanowicz, J.
Bartkowiak, R.
Powiązania:
https://bibliotekanauki.pl/articles/886757.pdf
Data publikacji:
2016
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
soil analysis
phosphatase
phosphatase activity
alkaline phosphatase
acid phosphatase
phosphorus
heavy metal content
mineral fertilization
Opis:
Assessment of soil phosphatase activity, phosphorus and heavy metals content depending on the mineral fertilization. The paper presents the results of research into the activity of alkaline and acid phosphatase, the content of available phosphorus, heavy metals and total organic carbon, against in soil with mineral fertilization only. The first experimental factor was phosphorus, potassium, magnesium, calcium and sulphur fertilization in six fertilizer combinations: 1 – PKMgCaS, 2 – KMgCaS, 3 – PMgCaS, 4 – PKCaS, 5 – PKMgS, 6 – PKMgCa. The second factor was made up of nitrogen fertilization at the rates of: 0, 50, 100, 150, 200, 250 kg·haˉ¹ of N. Increasing nitrogen rates and a lack of liming increased the soil acidity inhibiting alkaline phosphatase, decreasing the content of available phosphorus in soil. A lack of phosphorus fertilization resulted in an intensive increase in the activity of both alkaline and acid phosphatase in soil. Due to the experimental factors applied, the content of the heavy metals assayed was as follows: zinc > copper > lead > cadmium.
Ocena aktywności fosfataz, zawartości fosforu i metali ciężkich w zależności od nawożenia mineralnego. W pracy przedstawiono wyniki badań nad aktywnością fosfatazy zasadowej i kwasowej, zawartość przyswajalnego fosforu, metali ciężkich i węgla organicznego w glebie nawożonej wyłącznie mineralnie. Pierwszym czynnikiem doświadczenia było nawożenie fosforem, magnezem, wapniem i siarką w sześciu kombinacjach nawozowych: 1 – PKMgCaS, 2 – KMgCaS, 3 – PMgCaS, 4 – PKCaS, 5 – PKMgS, 6 – PKMgCa. Czynnikiem drugim było nawożenie azotem w dawkach: 0, 50, 100, 150, 200, 250 kg N·haˉ¹. Wzrastające dawki azotu i brak wapnowania spowodowały wzrost kwasowości gleby, powodując inhibicje fosfatazy alkalicznej, zmniejszenie zawartości fosforu przyswajalnego w glebie. Brak nawożenia fosforem spowodował intensywny wzrost aktywności zarówno fosfatazy alkalicznej, jak i kwaśnej w glebie. Pod wpływem zastosowanych czynników doświadczalnych zawartość metali ciężkich kształtowała się w sposób następujący: cynk > miedź > ołów > kadm.
Źródło:
Scientific Review Engineering and Environmental Sciences; 2016, 25, 2[72]
1732-9353
Pojawia się w:
Scientific Review Engineering and Environmental Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of different zinc doses and organic fertilization on soil’s enzymatic activity
Autorzy:
Kuziemska, B.
Wysokinski, A.
Klej, P.
Powiązania:
https://bibliotekanauki.pl/articles/1192090.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
zinc
organic materials
urease
dehydrogenases
acid phosphatase
alkaline phosphatase
Opis:
The enzymatic activity of soil was studied in a three-year pot experiment, where different amounts of zinc were introduced: 0, 200, 400, and 600 mg of Zn kg-1 of soil, as well as organic materials (control object, bovine manure, laying hens’ litter, spent mushroom substrate), used separately with the soil application dose of 2 g Corg kg-1 soil. Zinc and organic materials were used once, only in the first year of research, before sowing the test plant. In each year of the experiment, the test plant was orchard grass (Dactylis glomerata L.), harvested after 4 cuts. The activity of urease, dehydrogenases, as well as acid and alkaline phosphatase were determined in the soil collected after the last grass cutting, in each year of the study. Application of zinc to soil, regardless of its dose, resulted in a decrease in the activity of urease, dehydrogenase and alkaline phosphatase, although the significance of differences in relation to the control object was demonstrated only at higher doses, such as 400 and 600 mg Zn kg-1 soil. The study showed no negative effect of zinc on acid phosphatase activity. Organic fertilizers generally resulted in an increase in the enzymatic activity of the analyzed soil, and simultaneously limited the negative effects of higher zinc doses (400 and 600 mg Zn kg-1 of soil) on urease and dehydrogenase activity. During the consecutive years, urease and alkaline phosphatase activity decreased, while acid phosphatase activity increased. Dehydrogenase activity did not change significantly in the subsequent years of the study.
Źródło:
Journal of Elementology; 2020, 25, 3; 1089-1099
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
High sequence homology between protein tyrosine acid phosphatase from boar seminal vesicles and human prostatic acid phosphatase
Autorzy:
Wysocki, Paweł
Płucienniczak, Grażyna
Strzeżek, Jerzy
Powiązania:
https://bibliotekanauki.pl/articles/1040544.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
phosphorylation
boar
protein tyrosine phosphatase
seminal plasma
prostatic acid phosphatase
Opis:
Boar seminal vesicle protein tyrosine acid phosphatase (PTAP) and human prostatic acid phosphatase (PAP) show high affinity for protein phosphotyrosine residues. The physico-chemical and kinetic properties of the boar and human enzymes are different. The main objective of this study was to establish the nucleotide sequence of cDNA encoding boar PTAP and compare it with that of human PAP cDNA. Also, the amino-acid sequence of boar PTAP was compared with the sequence of human PAP. PTAP was isolated from boar seminal vesicle fluid and sequenced. cDNA to boar seminal vesicle RNA was synthesized, amplified by PCR, cloned in E. coli and sequenced. The obtained N-terminal amino-acid sequence of boar PTAP showed 92% identity with the N-terminal amino-acid sequence of human PAP. The determined sequence of a 354 bp nucleotide fragment (GenBank accession number: GQ184596) showed 90% identity with the corresponding sequence of human PAP. On the basis of this sequence a 118 amino acid fragment of boar PTAP was predicted. This fragment showed 89% identity with the corresponding fragment of human PAP and had a similar hydropathy profile. The compared sequences differ in terms of their isoelectric points and amino-acid composition. This may explain the differences in substrate specificity and inhibitor resistance of boar PTAP and human PAP.
Źródło:
Acta Biochimica Polonica; 2009, 56, 3; 481-486
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enzymatic activity in soil treated with exogenous organic matter
Autorzy:
Siebielec, Sylwia
Siebielec, Grzegorz
Ukalska-Jaruga, Aleksandra
Urbaniak, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/2148185.pdf
Data publikacji:
2021-12-30
Wydawca:
Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy
Tematy:
dehydrogenases
acidic phosphatase
alkaline phosphatase
manure
sewage sludge
bottom sediment
Opis:
The content of organic matter affects the quality and productivity of agricultural soils. Due to the shortage of manure as the basic source of organic matter for agricultural soils, al- ternative and commonly available exogenous sources of organic matter, such as sewage sludge and bottom sediments, are taken into account. The aim of the study was to evaluate the effect of the addition of various types of manure, municipal sewage sludge and bottom sediments on the enzymatic activity of soils (dehydrogenases, acidic and alkaline phosphatases). Manure and sludge were applied to the soil in two doses, corresponding to 20 and 40 tons of dry matter of material per ha. The control was soil fertilized with ammonium nitrate in the amount corresponding to 170 kg N per ha. The experiment included 2 different variants of the same fertilization combinations: without plants and with a test plant (Sinapis alba L.). The greenhouse experiment showed the stimulating effect of one of the municipal sewage sludge and poultry manure. On the other hand, the introduction of bottom sediments to the soil limited its enzymatic activity.
Źródło:
Polish Journal of Agronomy; 2021, 47; 87-94
2081-2787
Pojawia się w:
Polish Journal of Agronomy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Remediation of soil contaminated with cadmium
Autorzy:
Zaborowska, M.
Kucharski, J.
Wyszkowska, J.
Powiązania:
https://bibliotekanauki.pl/articles/958240.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
soil contamination
cadmium
alkaline phosphatase
acid phosphatase
soil microorganism
soil remediation
Opis:
The search for the best solutions to restore soil balance is essential for attaining a stable and sustainable agricultural development worldwide. This research, which makes a contribution to these investigations, focuses on four substances (two innovative ones: basalt flour, brown algal extract, and two classic soil improvers: finely ground barley straw and compost) which can potentially alleviate the inhibitory effect of Cd2+ on the soil environment. The following were analyzed: the activity of acid phosphatase and alkaline phosphatase, counts of Pseudomonas sp., cellulolytic bacteria, copiotrophic bacteria and copiotrophic spore-forming bacteria, and the yield of spring barley. Cadmium (Cd2+) was applied as CdCl2 · 2.5H2O in the following doses: 0, 4, 40, 80, 120, 160, and 200 mg Cd2+ kg-1 of soil. For a more complete assessment of the soil, its biochemical properties and the counts of microorganisms were scrutinized with the following indices: RS – soil resistance, R:S – rhizosphere effect and EF – fertilization effect of the contamination alleviating substances. It was found that alkaline phosphatase is more sensitive to cadmium contamination of the soil than acid phosphatase. Cadmium did not exert any inhibitory effect on the number of microorganisms present or the yield of spring barley. Cellulolytic bacteria were the least sensitive to stress associated with the accumulation of high cadmium doses in the soil, whereas copiotrophic bacteria were the most sensitive microorganisms to the above stressor. The ability of cadmium-polluted soil to restore homeostasis depended on the type of a soil improver and the level of soil contamination. Negative consequences of cadmium pollution were effectively mitigated by straw, but less so by brown algal extract and basalt flour.
Źródło:
Journal of Elementology; 2015, 20, 3
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Some specific and non-specific phosphatases in the sporocyst of Fasciola hepatica. 3. Enzymes associated with glycolysis
NIEKTÓRE SPECYFICZNE I NIESPECYFICZNE FOSFATAZY W SPOROCYSTACH FASCIOLA HEPATICA. III. ENZYMY ZWIĄZANE Z GLIKOLIZĄ
Autorzy:
Humiczewska, M.
Powiązania:
https://bibliotekanauki.pl/articles/2148926.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
glucose-6-phosphatase
aldolase
enzyme
sporocyst
glycolytic enzyme
Fasciola hepatica
phosphatase
hexokinase
Opis:
Using histochemical and cytophotometric methods, enzymes associated with anaerobie glycolysis: glucose-6-phosphatase, hexokinase, and aldolase in the developing sporocysts of Fasciola hepatica were studied. Highest activity of these enzymes was found in the germ balls in the sporocysts, at all phases of their development, which is related to intensive proliferation and differentiation of the embryos developing inside the germ balls.
Źródło:
Wiadomości Parazytologiczne; 1997, 43, 4; 411-423
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Some specific and non-specific phosphatases in the sporocyst of Fasciola hepatica. 3. Enzymes associated with glycolysis
Autorzy:
Humiczewska, M
Powiązania:
https://bibliotekanauki.pl/articles/837871.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
glucose-6-phosphatase
aldolase
enzyme
sporocyst
glycolytic enzyme
Fasciola hepatica
phosphatase
hexokinase
Opis:
Using histochemical and cytophotometric methods, enzymes associated with anaerobie glycolysis: glucose-6-phosphatase, hexokinase, and aldolase in the developing sporocysts of Fasciola hepatica were studied. Highest activity of these enzymes was found in the germ balls in the sporocysts, at all phases of their development, which is related to intensive proliferation and differentiation of the embryos developing inside the germ balls.
Źródło:
Annals of Parasitology; 1997, 43, 4
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Kinetic intermediates of unfolding of dimeric prostatic phosphatase
Autorzy:
Kuciel, Radosława
Mazurkiewicz, Aleksandra
Dudzik, Paulina
Powiązania:
https://bibliotekanauki.pl/articles/1041089.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
dimer
prostatic phosphatase
protein unfolding
Opis:
Kinetics of guanidine hydrochloride (GdnHCl)-induced unfolding of human prostatic acid phosphatase (hPAP), a homodimer of 50 kDa subunit molecular mass was investigated with enzyme activity measurements, capacity for binding an external hydrophobic probe, 1-anilinonaphtalene-8-sulfonate (ANS), accessibility of thiols to reaction with 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and 2-(4'-maleimidylanilino)naphthalene-6-sulfonate (MIANS) and ability to bind Congo red dye. Kinetic analysis was performed to describe a possible mechanism of hPAP unfolding and dissociation that leads to generation of an inactive monomeric intermediate that resembles, in solution of 1.25 M GdnHCl pH 7.5, at 20°C, in equilibrium, a molten globule state. The reaction of hPAP inactivation in 1.25 M GdnHCl followed first order kinetics with the reaction rate constant 0.0715 ± 0.0024 min-1 . The rate constants of similar range were found for the pseudo-first-order reactions of ANS and Congo red binding: 0.0366 ± 0.0018 min-1 and 0.0409 ± 0.0052 min-1, respectively. Free thiol groups, inaccessible in the native protein, were gradually becoming, with the progress of unfolding, exposed for the reactions with DTNB and MIANS, with the pseudo-first-order reaction rate constants 0.327 ± 0.014 min-1 and 0.216 ± 0.010 min-1, respectively. The data indicated that in the course of hPAP denaturation exposure of thiol groups to reagents took place faster than the enzyme inactivation and exposure of the protein hydrophobic surface. This suggested the existence of a catalytically active, partially unfolded, but probably dimeric kinetic intermediate in the process of hPAP unfolding. On the other hand, the protein inactivation was accompanied by exposure of a hydrophobic, ANS-binding surface, and with an increased capacity to bind Congo red. Together with previous studies these results suggest that the stability of the catalytically active conformation of the enzyme depends mainly on the dimeric structure of the native hPAP.
Źródło:
Acta Biochimica Polonica; 2007, 54, 2; 371-377
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ selenu i fluoru na aktywność fosfataz w glebie lekkiej
Effect of selenium and fluoride on phoshatase activities in sandy soil
Autorzy:
Onyszko, M.
Telesiński, A.
Powiązania:
https://bibliotekanauki.pl/articles/401499.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
fosfatazy
fluor
gleba
selen
wskaźnik oporności
acid phosphatase
alkaline phosphatase
fluoride
soil
selenium
resistance index
Opis:
Celem podjętych badań było określenie oddziaływania związków fluoru oraz selenu (na dwóch stopniach utlenienia: IV i VI), wprowadzonych osobno oraz w mieszaninie na aktywność fosfatazową gleby lekkiej. Doświadczenie przeprowadzono w warunkach laboratoryjnych na piasku gliniastym o zawartości węgla organicznego 8,7 g/kg, do którego wprowadzono w różnych kombinacjach fluor (10 mmol/kg) oraz selen (0,05 mmol/kg). W odstępach tygodniowych przez okres 28 dni oznaczano spektrofotometrycznie aktywność fosfatazy alkalicznej i fosfatazy kwaśnej. Na podstawie otrzymanych wyników obliczono wskaźniki oporności enzymów (RS). Wykazano że wprowadzenie do gleby fluoru i selenu spowodowało istotne statystycznie obniżenie aktywności oznaczanych enzymów. Aktywność fosfatazy alkalicznej charakteryzowała się większą wrażliwością na obecność fluoru i selenu w glebie niż fosfataza kwaśna. Dla fosfatazy alkalicznej największe obniżenie RS wystąpiło po aplikacji fluoru wraz z selenem na stopniu utlenienia IV, a dla fosfatazy kwaśnej – fluoru wraz z selenem na stopniu utlenienia VI.
The aim of the study was to determine the impact of fluoride and selenium (in two oxidation states IV and VI), introduced separately and in a mixture on soil phosphatase activity soil. The experiment was carried out in laboratory conditions on loamy sand samples with organic carbon content 8.7 g/kg. Various combinations of fluoride (10 mmol kg) and selenium (0.05 mmol/kg) were added into soil samples. Over a period of 28 days, at weekly intervals. Alkaline phosphatase and acid phosphatase activities were determined by spectrophotometry. Based on the obtained results, the resistance index (RS) for enzymes was calculated. It was found that the addition of fluoride and selenium into the soil resulted in a statistically significant decrease of assayedenzyme activities. Alkaline phosphatase activity was more sensitive to the presence of fluoride and selenium in the soil than acid phosphatase. The values of RS were lowest for alkaline phosphatase in soil treated with fluoride and selenium in the oxidation state IV, and for acid phosphatase in soil treated with fluoride and selenium in the oxidation state VI.
Źródło:
Inżynieria Ekologiczna; 2015, 43; 109-114
2081-139X
2392-0629
Pojawia się w:
Inżynieria Ekologiczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Difficulties in diagnosis and treatment of Paget’s disease
Autorzy:
Kawalec, Aleksandra
Zdrojowy-Wełna, Aleksandra
Bednarek-Tupikowska, Grażyna
Powiązania:
https://bibliotekanauki.pl/articles/551935.pdf
Data publikacji:
2016
Wydawca:
Stowarzyszenie Przyjaciół Medycyny Rodzinnej i Lekarzy Rodzinnych
Tematy:
Paget’s disease
alkaline phosphatase
bisphosphonates.
Źródło:
Family Medicine & Primary Care Review; 2016, 4; 501-505
1734-3402
Pojawia się w:
Family Medicine & Primary Care Review
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of tartaric acid on conformation and stability of human prostatic phosphatase: An infrared spectroscopic and calorimetric study.
Autorzy:
Bem, Sławomir
Ostrowski, Włodzimierz
Powiązania:
https://bibliotekanauki.pl/articles/1044106.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
secondary structure
prostate
acid phosphatase
tartrate
Opis:
The solution structure and thermal stability of human prostatic acid phosphatase (hPAP) in the absence and in the presence of tartaric acid were studied by Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC). The temperature dependence of the infrared spectrum and DSC scans indicate that hPAP undergoes thermal unfolding at a temperature between 49.5 and 52.5°C. Binding of tartaric acid does not lead to major changes in the secondary structure of hPAP, however, hPAP with bound tartaric acid shows a significantly increased thermal stability. These results helped to better understand the mechanism of hPAP unfolding at the elevated temperature.
Źródło:
Acta Biochimica Polonica; 2001, 48, 3; 755-762
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Impact of chlortetracycline and sulfapyridine antibiotics on soil enzyme activities
Autorzy:
Molaei, Ali
Lakzian, Amir
Datta, Rahul
Haghnia, Gholamhosain
Astaraei, Alireza
Rasouli-Sadaghiani, MirHassan
T. Ceccherini, Maria
Powiązania:
https://bibliotekanauki.pl/articles/972897.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
alkaline phosphatase
chlortetracycline
dehydrogenase
sulfapyridine
urease
Opis:
Pharmaceutical antibiotics are frequently used in the livestock and poultry industries to control infectious diseases. Due to the lack of proper guidance for use, the majority of administrated antibiotics and their metabolites are excreted to the soil environment through urine and feces. In the present study, we used chlortetracycline and sulfapyridine antibiotics to screen out their effects on dehydrogenase, alkaline phosphatase and urease activity. Factorial experiments were conducted with different concentrations of antibiotic (0, 10, 25 and 100 mg kg-1 of soil) mixed with soil samples, and the enzyme activity was measured at intervals of 1, 4 and 21 days. The results show that the chlortetracycline and sulfapyridine antibiotics negatively affect the dehydrogenase activity, but the effect of sulfapyridine decreases with time of incubation. Indeed, sulfapyridine antibiotic significantly affect the alkaline phosphatase activity for the entire three-time interval, while chlortetracycline seems to inhibit its activity within 1 and 4 days of incubation. The effects of chlortetracycline and sulfapyridine antibiotics on urease activity appear similar, as they both significantly affect the urease activity on day 1 of incubation. The present study concludes that chlortetracycline and sulfapyridine antibiotics have harmful effects on soil microbes, with the extent of effects varying with the duration of incubation and the type of antibiotics used.
Źródło:
International Agrophysics; 2017, 31, 4
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Plasma membrane homing of tissue nonspecific alkaline phosphatase under the influence of 3-hydrogenkwadaphnin, an antiproliferative agent from Dendrostellera lessertii
Autorzy:
Sadeghirizi, Akram
Yazdanparast, Razieh
Powiązania:
https://bibliotekanauki.pl/articles/1041081.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
alkaline phosphatase
anti-proliferative
Dendrostellera lessertii
Opis:
Several mammalian enzymes are anchored to the outer surface of the plasma membrane by a covalently attached glycosylphosphatidylinositol (GPI) structure. These include acetylcholinesterase, alkaline phosphatase (AP) and 5'-nucleotidase among other enzymes. Recently, it has been reported that these membrane enzymes can be released into the serum by the GPI-dependent phospholipase D under various medical disturbances such as cancer and/or by chemical and physical manipulation of the biological systems. Treatment of MCF-7 cells with two consecutive effective concentrations of 3-hydrogenkwadaphnin (3-HK, 3 nM) for 48 h enhanced membrane AP activity by almost 330% along with a 40% reduction in the AP activity of the cell culture medium. In addition, our data indicate that 3-HK is capable of inducing mainly the tissue-nonspecific alkaline phosphatase (TNAP) isoenzyme, along with enhancing its thermostability. These findings, besides establishing a correlation between the antiproliferative activity of 3-HK and the extent of plasma membrane AP activity, might assist in the development of new diagnostic tools for following cancer medical treatments.
Źródło:
Acta Biochimica Polonica; 2007, 54, 2; 323-329
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structural research of thermosensitive chitosan-collagen gels containing alp
Autorzy:
Skwarczyńska, Agata
Biniaś, Dorota
Modrzejewska, Zofia
Powiązania:
https://bibliotekanauki.pl/articles/1034675.pdf
Data publikacji:
2016
Wydawca:
Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
Tematy:
alkaline phosphatase
chitosan
collagen
thermosensitive hydrogels
Opis:
Introducing collagen, which is basic ingredient of bone tissue, into the structure of chitosan gels which are formed at the physiological body temperature, is aimed at creating the so-called biomimetic structures, i.e. close in their composition to the natural composition of bone tissue. Within the research the influence of collagen on structural properties of thermosensitive chitosan gels and the influence of ALP on structural properties of chitosan and chitosan-collagen gels was determined.
Źródło:
Progress on Chemistry and Application of Chitin and its Derivatives; 2016, 21; 176-186
1896-5644
Pojawia się w:
Progress on Chemistry and Application of Chitin and its Derivatives
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 92

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