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Wyświetlanie 1-49 z 49
Tytuł:
Induced isozyme polymorphism in spring barley mutants
Autorzy:
Kucharska, M
Maluszynski, M
Powiązania:
https://bibliotekanauki.pl/articles/2047711.pdf
Data publikacji:
1996
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
polymorphism
isoenzyme
barley
induced mutant
mutant
Opis:
The usefulness of mutagenic treatment to enlarge isozymic variability of barley and the use of induced mutants for genetic analysis were evaluated. N-methyl-N-nitroso urea, sodium azide and gamma rays were employed as mutagenic agents. Electrophoretic assays of 3848 M₂ seedlings obtained by chemical mutagenic treatment of the spring barley cultivars Dema, Aramir, Bielik and 3100 M₂ seedlings obtained by physical mutagenic treatment of the cv. Dema revealed 70 isozymic mutants, which represent 30 separate mutants in 25 M₁ plants. Most of mutations (27) were induced by chemical mutagen at polymorphic esterase loci. The occurrence of induced mutants at monomorphic loci, Got2 and Lap2, made it possible to perform genetic analysis of those loci in barley including mapping respective genes within chromosomes.
Źródło:
Journal of Applied Genetics; 1996, 37, 1; 1-9
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of EMS on induced morphological mutants and chromosomal variation in cowpea (Vigna unguiculata (L.) Walp)
Autorzy:
Gnanamurthy, S.
Dhanavel, D.
Powiązania:
https://bibliotekanauki.pl/articles/11505.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
ethyl methanesulphonate
morphological mutant
mutant
chromosome variation
cowpea
Vigna unguiculata
Opis:
Effect of EMS (ethyl methane sulphonate) on induced morphological mutants and chromosomal variation in cowpea was studied using five different doses of mutagen along with a control in randomized blocked design with three replications. The morphological mutants there are two types of viable and chlorophyll mutants. Viable mutant contains tall, dwarf, early maturity, late maturity, leaf mutants pod mutant and flower mutants. The frequency of chlorophyll mutant contains albino, xantha and viridis. This concentration can damage or modify important components of plant cells and have been reported to affect the morphology, anatomy, biochemistry and physiology of plants differentially depending on the concentration level. These effects include changes in the cellular structure and metabolism of the plants e.g., dilation of thylakoid membranes, alteration in photosynthesis, modulation of the antioxidative system and accumulation of phenolic compounds. The morphological and chromosomal variation was found to be mutagen sensitive in somatic cells of cowpea. It was found to increase with increasing the concentration of EMS in Cowpea plants. The chemical mutagen like ethyl methane sulphonate induces high frequency of chromosomal changes like anaphasic bridge; anaphasic laggard, anaphasic bridge and clumbing of chromosome were including control plants also observed.
Źródło:
International Letters of Natural Sciences; 2014, 17
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphological and Cytological Characteristics of Inflorescence Mutants in Alfalfa (Medicago Sativa L. Sl).
Autorzy:
Weigt, Dorota
Broda, Zbigniew
Lira, Jarosław
Mikołajczyk, Sylwia
Powiązania:
https://bibliotekanauki.pl/articles/2199056.pdf
Data publikacji:
2009-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
alfalfa
seed weight
long peduncle mutant
top flowering mutant
Opis:
The presented study analyzed selected biometric characteristics of inflorescences, seed yield structure characters, and discussed cytological analyses concerning two spontaneous inflorescence mutants in alfalfa: top flowering (plants with the “tf”gene) and long peduncle mutants (plants with the “lp” gene). Analyses were conducted in 2005 and 2006 and the results were subjected to a one-way statistical analysis with the use of Tukey’s test...
Źródło:
Plant Breeding and Seed Science; 2009, 60; 13-22
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Variability of yield structure and of physical traits determining lodging resistance in barley mutants
Autorzy:
Rybinski, W.
Jezowski, S.
Krajewski, P.
Powiązania:
https://bibliotekanauki.pl/articles/24434.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
yield structure
physical parameter
stem
lodging resistance
mutant
barley mutant
Źródło:
International Agrophysics; 1998, 12, 3
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of gamma irradiation on plants in interaction with arbuscular mycorrhizae
Autorzy:
Ene, M.
Trandafir, L.
Alexandru, M.
Powiązania:
https://bibliotekanauki.pl/articles/148043.pdf
Data publikacji:
2009
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
tomato
mutant
gamma irradiation
mycorrhiza defective
Opis:
Arbuscular mycorrhizae are endogenous fungi that form symbiotic association with the vast majority of vascular plants. They help to increase the nutrient uptake, especially phosphorus and to exploit a larger area of soil. Also, they improve soil aggregation, contribute to nutrient cycling processes in plants and improve their tolerance of heavy metal contamination or drought, as well as their susceptibility to root pathogens or herbivores. These fungi colonize the root epidermis and outer cortical layers and release bioactive molecules. The transcriptome and the proteome of plants are substantially altered. They have evolved multiple mechanisms that result in improvements in plant resistance to disease and plant growth and productivity, being included in a lot of combination of biofertilizers. In order to study these mechanisms, gamma irradiation was used to create mutants that are defective in their association. Culture under in vitro conditions is presented as method for maintaining the useful mutants in pure line.
Źródło:
Nukleonika; 2009, 54, 2; 89-92
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of laser beams on the variability of traits in spring barley
Autorzy:
Rybinski, W.
Powiązania:
https://bibliotekanauki.pl/articles/24451.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
variability
yield structure
chlorophyll mutant
laser
barley
biostimulation effect
genetic change
mutation
mutant
spring barley
Hordeum vulgare
plant
Źródło:
International Agrophysics; 2000, 14, 2
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seed microstructure and genetic variation of characters in selected grass-pea mutants [Lathyrus sativus L.]
Autorzy:
Rybinski, W.
Blaszczak, W.
Fornal, J.
Powiązania:
https://bibliotekanauki.pl/articles/25396.pdf
Data publikacji:
2006
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
microstructure
seed microstructure
plant property
Lathyrus sativus
selected mutant
grass pea
mutant
chemomutagenesis
seed
genetic variation
Źródło:
International Agrophysics; 2006, 20, 4
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The essential function of Swc4p - a protein shared by two chromatin-modifying complexes of the yeast Saccharomyces cerevisiae - resides within its N-terminal part
Autorzy:
Miciałkiewicz, Arkadiusz
Chełstowska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1040724.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
chromatin remodeling complexes
histone acetylation
mutagenesis
mutant phenotype
Opis:
The Swc4p protein, encoded by an essential gene, is shared by two chromatin-remodeling complexes in Saccharomyces cerevisiae cells: NuA4 (nucleosome acetyltransferase of H4) and SWR1. The SWR1 complex catalyzes ATP-dependent exchange of the nucleosomal histone H2A for H2AZ (Htz1p). The activity of NuA4 is responsible mainly for the acetylation of the H4 histone but also for the acetylation of H2A and H2AZ. In this work we investigated the role of the Swc4p protein. Using random mutagenesis we isolated a collection of swc4 mutants and showed that the essential function of Swc4p resides in its N-terminal part, within the first 269 amino acids of the 476-amino acid-long protein. We also demonstrated that Swc4p is able to accommodate numerous mutations without losing its functionality under standard growth conditions. However, when swc4 mutants were exposed to methyl methanesulfonate (MMS), hydroxyurea or benomyl, severe growth deficiencies appeared, pointing to an involvement of Swc4p in many chromatin-based processes. The mutants' phenotypes did not result from an impairment of histone acetylation, as in the mutant which bears the shortest isolated variant of truncated Swc4p, the level of overall H4 acetylation was unchanged.
Źródło:
Acta Biochimica Polonica; 2008, 55, 3; 603-612
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Parallel Mutant Execution Techniques in Mutation Testing Process for Simulink Models
Autorzy:
Hanh, L. T. M.
Binh, N. T.
Tung, K. T.
Powiązania:
https://bibliotekanauki.pl/articles/307992.pdf
Data publikacji:
2017
Wydawca:
Instytut Łączności - Państwowy Instytut Badawczy
Tematy:
mutant execution
mutation testing
parallel processing
software testing
Opis:
Mutation testing – a fault-based technique for software testing – is a computationally expensive approach. One of the powerful methods to improve the performance of mutation without reducing effectiveness is to employ parallel processing, where mutants and tests are executed in parallel. This approach reduces the total time needed to accomplish the mutation analysis. This paper proposes three strategies for parallel execution of mutants on multicore machines using the Parallel Computing Toolbox (PCT) with the Matlab Distributed Computing Server. It aims to demonstrate that the computationally intensive software testing schemes, such as mutation, can be facilitated by using parallel processing. The experiments were carried out on eight different Simulink models. The results represented the efficiency of the proposed approaches in terms of execution time during the testing process.
Źródło:
Journal of Telecommunications and Information Technology; 2017, 4; 90-100
1509-4553
1899-8852
Pojawia się w:
Journal of Telecommunications and Information Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation, identification and attenuation of a pathogenic duck hepatitis virus type 1 in China, and complete genomic sequence comparison between the embryo-passaged, attenuated derivatives and their parent
Autorzy:
Liu, X.
Kong, X.
Powiązania:
https://bibliotekanauki.pl/articles/2087586.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
duck hepatitis virus 1
pathogenicity
attenuation
mutant site
Źródło:
Polish Journal of Veterinary Sciences; 2019, 1; 163-171
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Two Gln187 mutants of human soluble APRIL inhibit proliferation of lung carcinoma A549 cells
Autorzy:
Dai, Shuangshuang
Zheng, Yingru
Chen, Bin
Gao, Min
Zhang, Yan
Zhang, Li
Gong, Wei
He, Fengtain
Powiązania:
https://bibliotekanauki.pl/articles/1040492.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
APRIL
Gln187 mutant of sAPRIL
anti-tumor activity
Opis:
Soluble APRIL (sAPRIL), the active form of a proliferation-inducing ligand (APRIL), is implicated in the proliferation of tumor cells. Suppressing APRIL function has been considered as a potential strategy for the therapy of APRIL-associated tumors. In the present study, we generated human sAPRIL and its two mutants, Gln187-D-sAPRIL (Gln187 deleted) and Gly187-sAPRIL (Gln187 replaced by Gly). In vitro experiments showed that the two mutants had similar specific binding capacity to lung carcinoma A549 cells compared to the wild-type sAPRIL, and both, especially Gly187-sAPRIL, exhibited significant antagonistic effect on sAPRIL-induced tumor cell proliferation in a dose-dependent manner, which might be predominantly mediated by blocking sAPRIL-induced MEK and ERK phosphorylation but not p38MAPK or JNK signaling. In vivo experiments with nude mice bearing A549 cell-derived xenograft tumor showed that only the Gly187-sAPRIL mutant could significantly suppress the tumor growth. These results suggest that Gln187 may be a crucial amino acid in APRIL-mediated tumor cell proliferation via the MEK-ERK signaling pathway and that the sAPRIL mutants may serve as novel potential antagonists of APRIL for the therapy of APRIL-associated cancers.
Źródło:
Acta Biochimica Polonica; 2009, 56, 4; 703-710
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Charakterystyka mutantow soi otrzymywanych po zastosowaniu mutagenow chemicznych i fizycznych
Autorzy:
Nawracala, J
Powiązania:
https://bibliotekanauki.pl/articles/834323.pdf
Data publikacji:
2000
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
mutanty
soja
genetyka roslin
mutageneza
mutant
soybean
plant genetics
mutagenesis
Źródło:
Rośliny Oleiste - Oilseed Crops; 2000, 21, 1; 33-45
1233-8273
Pojawia się w:
Rośliny Oleiste - Oilseed Crops
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Estimation of genetic variation of traits and physical properties of seeds for grass pea mutants [Lathyrus sativus L.]
Autorzy:
Rybinski, W.
Szot, B.
Pokora, L.
Powiązania:
https://bibliotekanauki.pl/articles/25857.pdf
Data publikacji:
2004
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
Lathyrus sativus
physical property
grass pea
mutant
genetic variation
seed
Źródło:
International Agrophysics; 2004, 18, 4
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mutations modulate soluble carbohydrates composition in seeds of Lathyrus sativus L.
Autorzy:
Piotrowicz-Cieslak, A I
Rybinski, W.
Michalczyk, D.J.
Powiązania:
https://bibliotekanauki.pl/articles/57224.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
mutagenesis
soluble carbohydrate
seed
Lathyrus sativus
mutant
galactosyl cyclitol
Opis:
Seeds of Lathyrus sativus cv. Derek and Krab were used as biological material for induced mutagenesis. Three mutant lines were obtained from seeds of grass pea cv. Derek and 15 lines from mutagenised seeds of cv. Krab. Twelve ethanol-soluble carbohydrates were identified in the seeds. We have selected grass pea mutant lines with high oligosaccharides content (lines D4, K56, K25, and K7) and lines with low raffinose family oligosaccharides (RFO) content (lines K12, K29 and K13). Mutations changing the levels of RFO have not affected the contents of galactosyl cyclitols.
Źródło:
Acta Societatis Botanicorum Poloniae; 2008, 77, 4; 281-287
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Defective Brca2 influences topoisomerase I activity in mammalian cells*.
Autorzy:
Rahden-Staroń, Iwonna
Szumiło, Maria
Grosicka, Emilia
Kraakman - van der Zwet, Maria
Zdzienicka, Małgorzata
Powiązania:
https://bibliotekanauki.pl/articles/1043656.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
camptothecin
V-C8 mutant
topoisomerase I
Brca2 gene
Opis:
The Chinese hamster cell mutant V-C8 is defective in the Brca2 gene (Kraakman-van der Zwet et al., 2002, Cell Biol.; 22: 669). Here we report that V-C8 cells were 10-fold more sensitive to camptothecin, an inhibitor of topoisomerase I, than the parental V79 cells. The level of the relaxation activity of topoisomerase I in nuclear extracts was also lower (4-fold) in V-C8 than V79 cells, in spite of the fact that the level of the topoisomerase I protein was the same in these cells. The survival of V-C8 cells in the presence of camptothecin, the sensitivity of V-C8 topoisomerase I to camptothecin, and the level of the relaxation activity in V-C8 nuclear extract were almost completely restored by transfection of V-C8 cells with the murine Brca2 gene or by the transfer of human chromosome 13 providing the BRCA2 gene. These results indicate that the observed changes in the topoisomerase I activity in V-C8 are due to the defective function of the Brca2 gene.
Źródło:
Acta Biochimica Polonica; 2003, 50, 1; 139-144
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efekt heterozji cech ilościowych rzepaku ozimego (Brassica napus L.) u mieszańców jednozerowych linii wsobnych krzyżowanych z odmianą Californium
Effect of quantitative traits heterosis in winter rape (Brassica napus L.) hybrids between zero-erucic inbred lines crossed with the variety Californium
Autorzy:
Łuczkiewicz, Tadeusz
Bocianowski, Jan
Nawracała, Jerzy
Powiązania:
https://bibliotekanauki.pl/articles/2198277.pdf
Data publikacji:
2012-06-28
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
heterozja
linie mutacyjne
rzepak ozimy
heterosis
mutant lines
winter rape
Opis:
Cztery linie mutacyjne (białe płatki korony, wąskie płatki korony, dwie linie męsko sterylne) mające w nasionach wysoką zawartość glukozynolanów (około 50 μmol/g s.m), krzyżowano z odmianą Californium. Doświadczenie z formami rodzicielskimi i mieszańcami F1 założono w Rolniczym Gospodarstwie Doświadczalnym Dłoń. Przeprowadzono obserwacje cech morfolo¬gicznych, cech — komponentów plonu oraz sprawdzono zawartość tłuszczu, białka i glukozynolanów w nasionach. Wykonano analizę wariancji, analizę zmiennych kanonicznych oraz obliczono efekty heterozji poszczególnych cech w stosunku do odmiany Californium oraz średniej z obojga rodziców. Badane formy rodzicielskie i mieszańce F1 różniły się istotnie pod względem wszystkich (poza liczbą i masą nasion z rośliny) analizowanych cech ilościowych. Analiza zmiennych kanonicznych potwierdziła, że linie mutacyjne wyprowadzone z odmian jednozerowych znacznie różniły się od odmiany Californium pod względem wszystkich cech traktowanych łącznie. W trzech z czterech kombinacjach krzyżowania odnotowano istotne efekty heterozji dla większości analizowanych cech w tym dodatnie, istotne, efekty heterozji w stosunku do odmiany Californium stwierdzono dla cech komponentów plonu: liczby łuszczyn i nasion z rośliny oraz masy nasion z rośliny. Wyniki te potwierdzają hipotezę, że efekty heterozji są większe przy krzyżowaniu form oddalonych genetycznie.
Four mutated lines (white petals, narrow petals, 2 male sterile lines) with high (about 50 μmol/g d.m.) glucosinolate content in seeds were crossed with variety Californium. Experiment with parental forms and F1 hybrids was carried out in Experimental Station Dłoń. Observations of morphological traits and yield component traits were made, and contents of oil, protein and glucosinolate in seeds were measured. Analysis of variance, analysis of canonical variates and effects of heterosis in relation to parental mean and to variety Californium were calculated. Analyzed parental forms and F1 hybrids differed significantly in all traits except a number and weight of seeds per plant. Analysis of canonical variates confirmed that the mutated lines originated from zero-erucic varieties differed from variety Californium with regards to all analyzed traits taken jointly. In three from four cross combinations significant positive effects of heterosis were observed for the majority of traits including positive effects to variety Californium for yield component traits: number of pods and seeds per plant and weight of seeds per plant. These results confirm the hypothesis that heterosis effects is higher in hybrids between genetically distinct forms.
Źródło:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2012, 264; 67-77
0373-7837
2657-8913
Pojawia się w:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative study for effects of chemical mutagenesis on seed germination, survivability and pollen sterility in M1 and M2 generations of Dianthus
Autorzy:
Roychowdhury, Rajib
Alam, Molla Jaunatul Ferdousul
Bishnu, Sunanda
Dalal, Tinkari
Tah, Jagatpati
Powiązania:
https://bibliotekanauki.pl/articles/2199631.pdf
Data publikacji:
2012-04-19
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
chemical mutagen
Dianthus caryophyllus
mutant generation
pollen sterility
seed germination
Opis:
Chemical mutagenesis is an efficient tool used in mutation breeding programme for improving various vital characteristics in floricultural crop, like Dianthus. In this study, ethyl methane sulphonate (EMS), sodium azide (SA) and colchicine (COL) with three different concentrations (0.1%, 0.4% and 0.7%) were used to analyse their effect on seed germination behaviour, survivability and pollen sterility in both first (M1) and second (M2) mutant generations. It was noted that increase in the dose of EMS and SA, germination percentage and survivability were decreased; whereas colchicine doses were proportional to increase germination percentage at seedling stage, but they were not survived till maturity. In M1 and M2, higher lethality over control (44.3 and 32.89, respectively) was shown by 0.7% of SA and EMS, respectively. Pollen sterility was also increased with increasing mutagenic doses. The maximum pollen sterility was 71.8% and 61.1% for 0.7% COL in M1 and M2, respectively. So, the effect of chemical mutagenesis on biological parameters with SA (0.7%) treatment in M1 and EMS (0.7%) treatment in M2 were much more beneficial as compared to colchicine. For each studied parameter, chemical mutagenesis was higher in M1 than M2. Hence, for the first time in Dianthus, we reported that these mutagens can be used for improving the germination behaviour and the metrical traits in Dianthus cultivar.
Źródło:
Plant Breeding and Seed Science; 2012, 65; 29-38
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic variation of hordein polypeptides in grains of mutants of hulless spring barley [Hordeum vulgare L.] breeding line
Autorzy:
Kapala, A
Rybinski, W
Powiązania:
https://bibliotekanauki.pl/articles/2047707.pdf
Data publikacji:
1996
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
polypeptide spectrum
breeding line
barley
mutant
Hordeum vulgare
hordein polypeptide
hull
Opis:
Hordein polypeptides patterns of 146 mutants in M7 from a hulless spring barley breeding line were examined. The mutants were obtained after treatment of grains of Polish hulless spring barley breeding line, 1N/86, with the chemomutagens N-nitroso-N-methylurea and sodium azide. The mutants were distinguished from the parental line with regard to morphological and yield structure traits. SDS-PAGE of the main hordein fractions, B and C, showed that the electrophoretic patterns of the majority of the analysed mutants were similar to the hordein polypeptide spectrum of the parental line. However, specific hordein patterns were obtained from six of the mutants. In the previous study, the same mutants showed a significantly better feeding value as compared to hulled cultivars and the rest of the analysed mutants.
Źródło:
Journal of Applied Genetics; 1996, 37, 1; 29-35
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Estimation of genetic variability of yielding traits and physical properties of seeds of spring barley [Hordeum vulgare L.] mutants
Autorzy:
Rybinski, W.
Szot, B.
Powiązania:
https://bibliotekanauki.pl/articles/24735.pdf
Data publikacji:
2006
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
physical property
genetic variability
barley
mutant
spring barley
Hordeum vulgare
yielding trait
seed
Źródło:
International Agrophysics; 2006, 20, 3
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena zmienności fenotypowej i molekularnej okrągłonasiennej formy lędźwianu siewnego (Lathyrus sativus L.)
Estimation of variability on phenotypic and molecular level of spherical-seeded form of grasspea (Lathyrus sativus L.)
Autorzy:
Pankiewicz, Katarzyna
Rybiński, Wojciech
Kaczmarek, Zygmunt
Powiązania:
https://bibliotekanauki.pl/articles/2198161.pdf
Data publikacji:
2011-09-30
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
Lathyrus sativus
doświadczenia polowe
markery ISSR
mutant okrągłonasienny
skład chemiczny nasion
struktura plonowania
chemical composition of seeds
field trials
ISSR markers
spherical-seeded mutant
yield-contributing traits
Opis:
Obiekty badawcze stanowiły: mutant o okrągłych nasionach, odmiany Derek i Krab, jako potencjalny materiał z którego wywodzi się wyselekcjonowana okrągłonasienna forma oraz o obiekt kolekcyjny z Włoch (typowy przedstawiciel lędźwianów Południowej Europy). Zmienność cech na poziomie fenotypowym oceniano w oparciu o wyniki dwuletnich doświadczeń polowych w latach 2008 i 2009 założonych w czterech zróżnicowanych glebowo miejscowościach wykorzystując w tym celu wielocechową analizę statystyczną. Zmienność genetyczną wyrażoną stopniem podobieństwa genetycznego oceniano za pomocą markerów ISSR. Ponadto, oceniano skład chemiczny nasion w odniesieniu do zawartości białka, tłuszczu, profilu kwasów tłuszczowych oraz neurotoksyny — β-ODAP. Wykazano, że kulista forma lędźwianu pod względem takich cech jak: liczba rozgałęzień i strąków z rośliny, długość strąka, masa nasion ze strąka, liczba nasion z rośliny, a zwłaszcza masa 1000 nasion różni się istotnie od odmian Derek i Krab, a wszystkie trzy formy reprezentują inny morfotyp i poziom cech plonotwórczych w porównaniu z obiektem z Włoch. Ponadto, kulista forma charakteryzowała się wyższą niż u pozostałych form zawartością białka i tłuszczu, z dominującą zawartością kwasu oleinowego w profilu kwasów tłuszczowych. Wszystkie rodzime formy lędźwianu charakteryzowały się zbliżoną zawartością w nasionach neurotoksyny — β-ODAP z wyraźnie wyższą jej zawartością u formy z Włoch. Wykreślony dendrogram na podstawie wyników analizy ISSR wskazuje, że krajowe formy charakteryzują się znacznym podobieństwem genetycznym i wyraźną odrębnością od włoskiej formy. Wyniki na poziomie fenotypowym uzyskane z doświadczeń polowych wskazują ponadto, że spontaniczna mutacja kształtu nasion powstała z dużym prawdopodobieństwem w obrębie materiału populacyjnego z którego pochodzi odmiana Derek, aczkolwiek na poziomie molekularnym mutant wykazywał bliższe podobieństwo do odmiany Krab.
A mutant with spherical seeds was investigated together with two Polish cultivars: Derek and Krab (most probable ancestors of the mutant) as well as an Italian grasspee line, typical for South-Western Europe (Mediterranean Basin region). Variation of traits on phenotypic level was estimated basing on results obtained from field trials conducted in four locations in 2008 and 2009. The multivariate analysis method was used. Genetic variation, expressed by degree of genetic similarity, was established with the use of ISSR markers. Moreover, content of protein, fat, fatty acid composition and neurotoxin content (β-ODAP) in seeds was estimated. The obtained results indicated, that the spherical-seeded mutant, as compared to the cultivars Derek and Krab, was significantly different in the following traits: number of branches and pods per plant, pod length, weight of seeds per pod, seeds number per plant, as well as weight of 1000 seeds. Moreover, all three Polish forms represented different morphotype and yield structure parameters than the Italian line. The seeds of spherical-seeded mutant showed higher content of protein and fat as compared to another analyzed forms, with domination of oleic acid in fatty acids composition. All Polish accessions were similar in neurotoxin content in seeds, markedly lower in comparison to the Italian line. Results of hierarchical grouping (after use of the ISSR method) were presented on the dendrogram which indicated that the cultivars and spherical-seeded mutant showed high level of genetic similarity and markedly differed from the Italian line. The results obtained on the phenotypic level from field trials, estimated with use of multivariate statistics, indicated the population from which the cultivar Derek was derived as the most probable ancestor of the spherical seed mutant, however on the molecular level the mutant showed closer genetic similarity to the cultivar Krab.
Źródło:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2011, 260/261; 351-365
0373-7837
2657-8913
Pojawia się w:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Variability of seed protein composition of hulless mutants of spring barley [Hordeum vulgare L.]
Autorzy:
Kapala, A
Rybinski, W.
Powiązania:
https://bibliotekanauki.pl/articles/2048149.pdf
Data publikacji:
1995
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
grain
barley
crude fibre
protein
mutant
spring barley
hull
seed
nutritional value
Opis:
Nutritional value of protein in 146 mutants of hulless spring barley was estimated. Evaluation of protein quality was based on the portion of a relative content of protein fractions with different amino acid composition and, therefore, with different nutritional value. The ratio of albumins, globulins and glutelins to hordein, determining nutritional value of protein in the initial genotype was 1.1; in mutants it was within the range of 0.9-1.5. Several mutants with a high protein content and good nutritional value have been distinguished.
Źródło:
Journal of Applied Genetics; 1995, 36, 2; 129-135
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic variation in mutants of chilli (Capsicum annuum) revealed by RAPD marker
Autorzy:
Mullainathan, L.
Sridevi, A.
Umavathi, S.
Sanjai Gandhi, E.
Powiązania:
https://bibliotekanauki.pl/articles/11592.pdf
Data publikacji:
2014
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
genetic variation
mutant
chilli
Capsicum annuum
random amplified polymorphic DNA analysis
RAPD marker
spice
Opis:
The present study was under taken in order to analyze the chemical mutagenesis on Chilli germplasm. In this regard, K1 variety of chilli was subjected to different mutagenic concentration for inducing mutagenesis. The M3 plants exposed to EMS and DES to produce clear difference from the untreated control, thus indicating that mutagenic treatment produce polymorphic regions in the chilli. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall, tall with chlorophyll deficient, leaf, flower, GMS and DNA damages in maturity mutants were analyzed with control. Out of ten primers, four primers (PGF02, PGF03, PGF04 AND OP107) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 93 products showing an average of 9.3 bands.
Źródło:
International Letters of Natural Sciences; 2014, 06
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mechanical touch responses of Arabidopsis TCH1-3 mutant roots on inclined hard-agar surface
Autorzy:
Zha, G.
Wang, B.
Liu, J.
Yan, J.
Zhu, L.
Yang, X.
Powiązania:
https://bibliotekanauki.pl/articles/24565.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
mechanical touch response
mechanical touch simulation
Arabidopsis
TCH1-3 gene
mutant
root growth
Źródło:
International Agrophysics; 2016, 30, 1
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Intermedium mutants in barley [Hordeum vulgare L.] - diversity, interactions and plant breeding value
Autorzy:
Lundqvist, U
Lundqvist, A.
Powiązania:
https://bibliotekanauki.pl/articles/2044458.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mutant combination
gene interaction
diversity
barley
mutation
Hordeum vulgare
intermedium gene
plant breeding
Opis:
Mutation research has given an insight into the rather complex genetics of kernel rows in barley. At least 12 gene loci can act to promote the spike development, fertility, and kernel development. Mutants with such effects as sixrow and intermedium phenotypes show clear morphological distinctions among different loci, but also among different allelic mutants. These genes, without exception, are capable of unexpected synergistic reinforcing or disturbing intraction, the extremes being typically six-rowed or deformed spikes, respectively. The investigations have centered on 69 intermedium mutants, representing 9 loci, in double mutant combinations, in double combinations with the six-row gene hex-v, in triple combinations with hex-v, and in triple and quadruple mutant combinations. The effects of the interaction may differ among the three characters of lateral floret development, among intermedium loci, and among alleles of the particular locus. Particular types of gene interaction are indicated, particular loci being more competent than others, and the particular alleles being more competent in relation to the constellation of loci. Accumulation of intermedium genes in more complex gene systems leads to progressive promotion of lateral floret development, but there are indications that such systems may be more sensitive to environmental stress, leading to irregular or even deformed spike formation. Probably, representatives of the hex-v locus should form the fundamental constituent in the synthesis of gene systems with the most efficient promotion of lateral floret development in the breeding of six-row barley.
Źródło:
Journal of Applied Genetics; 1998, 39, 1; 85-96
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of selected grasspea mutants by generalized linear model
Analiza wybranych mutantów lędźwianu siewnego przy zastosowaniu uogólnionego modelu liniowego
Autorzy:
Bocianowski, J.
Bakinowska, E.
Rybinski, W.
Powiązania:
https://bibliotekanauki.pl/articles/9591.pdf
Data publikacji:
2008
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Katedra Zastosowań Matematyki i Informatyki
Tematy:
grass pea
Lathyrus sativus
mutant
fatty acid
seed
generalized linear model
logistic model
Źródło:
Colloquium Biometricum; 2008, 38
1896-7701
Pojawia się w:
Colloquium Biometricum
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Stability of the activity of Leuconostoc mutants in deep frozen concentrates
Autorzy:
Piątkiewicz, A.
Powiązania:
https://bibliotekanauki.pl/articles/1371449.pdf
Data publikacji:
1992
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
frozen starter
activity
Leuconostoc mesenteroides
fermentation technology
stability
dairy starter
mutant
Leuconostoc lactis
Źródło:
Polish Journal of Food and Nutrition Sciences; 1992, 01, 4; 57-66
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Modulation of the voltage-dependent anion-selective channel by cytoplasmic proteins from wild type and the channel depleted cells of Saccharomyces cerevisiae.
Autorzy:
Kmita, Hanna
Budzińska, Małgorzata
Stobienia, Olgierd
Powiązania:
https://bibliotekanauki.pl/articles/1043619.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
VDAC-depleted mutant
reconstituted system
Saccharomyces cerevisiae
voltage dependent anion selective channel (VDAC)
mitochondria
yeast
Opis:
It is well known that effective exchange of metabolites between mitochondria and the cytoplasm is essential for cell physiology. The key step of the exchange is transport across the mitochondrial outer membrane, which is supported by the voltage-dependent anion-selective channel (VDAC). Therefore, it is clear that the permeability of VDAC must be regulated to adjust its activity to the actual cell needs. VDAC-modulating activities, often referred to as the VDAC modulator, were identified in the intermembrane space of different organism mitochondria but the responsible protein(s) has not been identified as yet. Because the VDAC modulator was reported to act on VDAC of intact mitochondria when added to the cytoplasmic side it has been speculated that a similar modulating activity might be present in the cytoplasm. To check the speculation we used mitochondria of the yeast Saccharomyces cerevisiae as they constitute a perfect model to study VDAC modulation. The mitochondria contain only a single isoform of VDAC and it is possible to obtain viable mutants devoid of the channel (Δpor1). Moreover, we have recently characterised a VDAC-modulating activity located in the intermembrane space of wild type and Δpor1 S. cerevisiae mitochondria. Here, we report that the cytoplasm of wild type and Δpor1 cells of S. cerevisiae contains a VDAC-modulating activity as measured in a reconstituted system and with intact mitochondria. Since quantitative differences were observed between the modulating fractions isolated from wild type and Δpor1 cells when they were studied with intact wild type mitochondria as well as by protein electrophoresis it might be concluded that VDAC may influence the properties of the involved cytoplasmic proteins. Moreover, the VDAC-modulating activity in the cytoplasm differs distinctly from that reported for the mitochondrial intermembrane space. Nevertheless, both these activities may contribute efficiently to VDAC regulation. Thus, the identification of the proteins is very important.
Źródło:
Acta Biochimica Polonica; 2003, 50, 2; 415-424
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Role of Sinorhizobium meliloti glutaredoxins in nitrogen-fixing symbiosis
Autorzy:
Benyamina, S.
Alloing, G.
Rouhier, N.
Jacquot, J.
Puppo, A.
Frendo, P.
Powiązania:
https://bibliotekanauki.pl/articles/80782.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
Sinorhizobium meliloti
glutaredoxin
nitrogen-fixing symbiosis
bacteroid
Smgrx2 mutant strain
iron metabolism
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative stability analysis of D23N mutated Aβ
Autorzy:
Alred, E. J.
Scheele, E. G.
Berhanu, W. M.
Hansmann, U. H. E.
Powiązania:
https://bibliotekanauki.pl/articles/1935815.pdf
Data publikacji:
2014
Wydawca:
Politechnika Gdańska
Tematy:

structural polymorphism
molecular dynamics
parallel and anti-parallel β sheets
Iowa mutant
Opis:
Amyloid β (Aβ) is the subject of numerous studies due to its link to the devastating Alzheimer’s disease and it exists in a parallel structure in fibril aggregate. The Iowa mutant (D 23 N) Aβ posses a unique antiparallel fibril aggregate structure and can also form parallel structure. This structural difference, coupled with the fact that occurrence of the Iowa mutant is correlated with early onset Alzheimer’s, suggests to use these peptides as candidates for computational studies of the structural determinants of the toxicity of Alzheimer’s disease. In order to compare the two observed Aβ structural motifs, we designed a computational study to probe the factors that affect the stability of parallel and antiparallel aggregates. Since the structural changes may occur on a timescale beyond that sampled in traditional molecular dynamics (MD), we employed a techniques of scaling the mass to reduce the solution ’s viscosity and compared the results to regular molecular dynamics. The knowledge gained from this study could provide insight into the mechanism of selection for antiparallel and parallel two fold structures.
Źródło:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk; 2014, 18, 4; 365--371
1428-6394
Pojawia się w:
TASK Quarterly. Scientific Bulletin of Academic Computer Centre in Gdansk
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zmiennosc u soi [Glycine max L.Merrill] indukowana mutagenem chemicznym
Autorzy:
Sodkiewicz, T
Powiązania:
https://bibliotekanauki.pl/articles/833932.pdf
Data publikacji:
2000
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
mutanty
soja
genetyka roslin
zmiennosc
mutageny chemiczne
mutageneza
mutant
soybean
plant genetics
variability
chemical mutagen
mutagenesis
Źródło:
Rośliny Oleiste - Oilseed Crops; 2000, 21, 2; 539-546
1233-8273
Pojawia się w:
Rośliny Oleiste - Oilseed Crops
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antagonism of salicylic acid signalling on apoplastic reactive oxygen species signalling
Autorzy:
Xu, E.
Brosche, M.
Powiązania:
https://bibliotekanauki.pl/articles/80426.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
salicylic acid
antagonism
reactive oxygen species
signalling pathway
jasmonic acid
gene expression
dnd1 mutant
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Linkage of two mutant allozymes for Amp2 and Aat2 with marker loci on barley [Hordeum vulgare L.] chromosomes 1 and 6
Autorzy:
Kucharska, M
Kaczorowska, K.
Ali, E.S.
Powiązania:
https://bibliotekanauki.pl/articles/2048291.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
chromosome
starch
isoenzyme
barley
linkage
gel electrophoresis
mutant
allozyme
genetic analysis
Hordeum vulgare
genetic marker
Opis:
To saturate barley (Hordeum vulgare L.) genetic maps the linkage relationships of two isoenzyme loci Amp2 (aminopeptydase) and Aat2 (aspartate aminotransferase) with known genetic markers were investigated. Results of the genetic analysis support previous information on the localization of these loci on chromosome 1 and 6, respectively. The following recombination values were estimated: between locus Amp2 and T1-3b translocation break point 13.8 ± 2.1%, between locus Aat2 and translocation T6-7i, 17 ± 3.0% and between locus Aat2 and marker о 24.1 ± 3.0%.
Źródło:
Journal of Applied Genetics; 1998, 39, 2; 147-150
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cysteine-rich receptor-like kinases (CRK) in ROS signalling in Arabidopsis thaliana
Autorzy:
Gauthier, A.
Idanheimo, N.
Salojarvi, J.
Wrzaczek, M.
Kangasjarvi, J.
Powiązania:
https://bibliotekanauki.pl/articles/80229.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
receptor-like kinase
reactive oxygen species
signalling
Arabidopsis thaliana
protein-protein interaction
crk mutant
xanthine oxidase
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cytoembryological analysis of causes for poor seed set in alfalfa (Medicago sativa L.)
Autorzy:
Mol, R.
Weigt, D.
Broda, Z.
Powiązania:
https://bibliotekanauki.pl/articles/19337.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cytoembryology
seed
alfalfa
Medicago sativa
seed set
pollen tube
embryonic development
inflorescence mutant
pollen germination
plant breeding
Opis:
Poor seed set is a limiting factor in alfalfa breeding, as it slows the selection response. One strategy used to overcome this problem is to search for mutations of inflorescence morphology. Long-peduncle (lp), branched-raceme (br) and top-flowering (tf) inflorescence mutations increase the number of flowers per inflorescence, but they do not improve seed set per flower. Here we assessed pollen tube growth in styles of those inflorescence mutants and we observed embryo and endosperm development in seeds 1 to 16 days after pollination (DAP). The number of pollen tubes penetrating the style and the ovary was similar in all tested mutants and in the reference cultivar Radius. At 2 DAP, fertilized ovules were 2.7–3.9 times less numerous in certain inflorescence mutants than in the short-raceme cv. Radius. Ovule degeneration progressed at 2–4 DAP in all analyzed plants. Most ovules were not properly developed in the control cultivar (62%), nor in the forms with mutated inflorescence morphology (69–86%). The number of seeds per pod was lowest in the tf form despite its having the highest number of ovules per ovary. It appears that the number of ovules per pistil is not a crucial factor in seed set in alfalfa when fertilization efficiency is very low. Both poor fertilization and gradual ovule degeneration were factors causing poor seed set in the investigated alfalfa genotypes.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Estimation of induced mutation rates of four esterase genes in barley [Hordeum vulgare L.]
Autorzy:
Kucharska, M
Maluszynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/2044884.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
mutation frequency
isoenzyme
barley
hybrid
mutagen
enzyme system
plant morphology
mutant
Hordeum vulgare
esterase
estimation
seed
Opis:
Induced mutation rate of barley esterase loci has been estimated. Results suggested that about 3% of investigated M₁ spikes had seeds which gave rise to M₂ seedlings mutated in one of four esterase loci. M₁ plants were obtained after chemical treatment of seeds from two spring barley cultivars Aramir and Bielik. The majority of mutants were reconfirmed in the М₃ generation.
Źródło:
Journal of Applied Genetics; 1998, 39, 2; 141-145
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biosynteza beta-glukanaz przez mutanty Trichoderma reesei podczas hodowli okresowych
Autorzy:
Janas, P
Targonski, Z.
Rudnicka-Pilat, T.
Powiązania:
https://bibliotekanauki.pl/articles/826322.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Technologów Żywności
Tematy:
hodowla mikroorganizmow
mutanty
biosynteza
Trichoderma reesei
mikroorganizmy
beta-glukanazy
microorganism culture
mutant
biosynthesis
microorganism
beta-glucan
Opis:
Cztery mutanty T. reesei były testowane pod kątem produkcji beta-glukanaz podczas hodowli okresowych w obecności różnych źródeł węgla. Nadproducent enzymów autolitycznych Trichoderma viride F- 19 został użyty jako mutant porównawczy. Najwyższe aktywności beta-glukanaz oznaczono w filtratach otrzymanych po hodowlach T. viride F-19 i T. reesei VTT-D-78085 na pożywkach zawierających 1% mieszaniny glukanu i chityny w stosunku 1:1. Stwierdzono wysokie aktywności proteolityczne filtratów podczas hodowli mutantów w obecności 1% glukanu, które były związane z zanieczyszczeniem tego substratu substancjami białkowymi, działającymi indukcyjnie na produkcję tych enzymów. Beta - gluka- nazy produkowane przez T. reesei M-7 były odporne na glukozową represję kataboliczną.
Four Trichoderma reesei mutants have been tested during batch cultivations for beta-glucanases production on the medium with different source of carbon. Trichoderma viride F-19 has been used as high-lytic enzyme-production reference strain. The highest activities of beta-glucanases have been estimated in culture filtrates obtained after cultivation of T. reesei VTT-D-78085 and T. viride F-19 on the medium with 1% mixture of chitin and glucan at the ratio 1:1. High proteolytic activities of culture filtrates obtained during cultivation in the presence of 1% of glucan were connected with high protein content in this substrate which acting as inducer of production of these enzymes. Beta-glucanases produced by T. reesei M-7 were resistant to glucose catabolite repression.
Źródło:
Żywność Nauka Technologia Jakość; 2002, 09, 2; 30-42
1425-6959
Pojawia się w:
Żywność Nauka Technologia Jakość
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dynamics of excitation energy transfer in PSII particles of Arabidopsis thaliana mutant clpC1
Autorzy:
Adamiec, M.
Lucinski, R.
Gibasiewicz, K.
Giera, W.
Sipinska, W.
Jackowski, G.
Powiązania:
https://bibliotekanauki.pl/articles/80441.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
excitation energy transfer
Arabidopsis thaliana
clpC1 mutant
polypeptide
time-resolved fluorescence
regulatory protein
chloroplast
apoprotein
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
p53-dependent suppression of the human calcyclin gene (S100A6): the role of Sp1 and of NFκB
Autorzy:
Króliczak, Weronika
Pietrzak, Maciej
Puzianowska-Kuznicka, Monika
Powiązania:
https://bibliotekanauki.pl/articles/1040715.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
gene suppression
wild type and mutant p53
Sp1
calcyclin gene (S100A6)
NFκB
Opis:
Calcyclin (S100A6) is believed to participate in cell cycle control. It was, however, unclear if its expression depends on p53, a key regulator of apoptosis and cell cycle. We therefore performed transcription regulation assays in HeLa cells and found that wild type p53 suppressed the S100A6 promoter up to 12-fold in a dose-dependent manner. In contrast, the well-characterized V143A, R175H, R249S, and L344A p53 mutants cloned from human cancers suppressed this promoter with a 6 to 9-fold lower efficiency. All the sites mediating the p53-dependent suppression were contained in the -167 to +134 fragment of the S100A6 promoter. Separate overexpression of either Sp1 or of NFκB only partially counteracted the p53 inhibitory effect on the S100A6 promoter, while simultaneous overexpression of both these transactivators resulted in a complete abolishment of the p53 inhibitory effect on this promoter. Sp1 and NFκB binding to the probes resembling their putative binding sites present in the S100A6 promoter was decreased in the presence of wild type p53. We propose that the suppression of S100A6 is yet another mechanism by which p53 inhibits proliferation. Insufficient suppression of this gene by p53 mutants could well be responsible for calcyclin overexpression and cell cycle deregulation observed in cancer tissues.
Źródło:
Acta Biochimica Polonica; 2008, 55, 3; 559-570
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improving the monacolin K to citrinin production ratio in red yeast rice by an X-ray-induced mutant strain of Monascus purpureus
Autorzy:
Kanpiengjai, A.
Mahawan, R.
Pengnoi, P.
Lumyong, S.
Khanongnuch, C.
Powiązania:
https://bibliotekanauki.pl/articles/80836.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Red Yeast Rice
Monascus purpureus
solid state fermentation
citrinin
monacolin K
mutagenesis
X-ray
mutant strain
ionizing radiation
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2018, 99, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rice improvement, involving altered flower structure more suitable to cross-pollination, using in vitro culture in combination with mutagenesis
Autorzy:
Min, S K
Powiązania:
https://bibliotekanauki.pl/articles/2048292.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
japonica rice
radiation
China
mutagenesis
mutant
anther culture
in vitro
cross-pollination
hybridization
indica rice
somatic cell
Oryza sativa
rice
Opis:
Anther and somatic tissue culture in combination with mutagenesis were carried out to evaluate the efficiency of different mutagenic treatments of various in vitro culture materials, and to obtain some promising variants for rice improvement. Results indicated that in japonica rice radiation treatment of dry seeds and young panicles influenced the percentage of green plantlets regeneration from anther culture. Both treatments increased significantly the percentage of regenerated green plantlets in comparison with the control. Irradiation with 30 Gy of rice callus increased also the percentage of regenerated green plantlets. For indica rice, the combination of the suitable dose of gamma rays irradiation on seeds and an improved medium, increased the percentage of callus induction. This approach made it possible to use anther culture in indica rice breeding. Somatic tissue cultures combined with radiation-induced mutagenesis led to the development of a number of promising mutants including some new cytoplasm-nucleus interacting male-sterile lines with almost 100% stigma exsertion. Their development would be of practical significance for increasing the genetic diversity for production of hybrid rice.
Źródło:
Journal of Applied Genetics; 1998, 39, 2; 151-162
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic analysis of selected tomato [Lycopersicon esculentum Mill.] traits in crosses between cultivated lines and the nor mutant
Autorzy:
Seroczynska, A
Niemirowicz-Szczytt, K.
Powiązania:
https://bibliotekanauki.pl/articles/2044241.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
inheritance
shelf life
polygalacturonase gene
Lycopersicon esculentum
nor gene
mutant
heterozygote
tomato breeding
yield
hybrid
ripening
cross
earliness
genetic analysis
fruit weight
Opis:
The aim of the study was to investigate the delayed ripening caused by the nor gene and its possible interaction with selected traits observed in two combinations, namely, Dw₄ x nor and C36 x nor. The lines Dw₄ and C36 differed from each other in the majority of examined characters. There was no significant effect of reciprocal crosses on these characters (total yield, number of fruits, fruit weight, locule number, earliness and fruit shelf life). In Dw₄ x nor fruit weight was inherited with incomplete dominance of small fruit, while in C36 x nor the inheritance proved to be intermediate. This important statement made it possible to distinguish the hybrids characterised by fruit weight intermediate in relation to the parental forms. The experiments attested to the high significance of additive effects for fruit weight, periods from sowing to ripening and from flowering to ripening in both combinations and for locule number in Dw₄ x nor. It was also found that the nor gene slightly delayed the onset of fruit ripening but did not affect such traits as total yield, number and weight of fruits, locule number and period from sowing to flowering. In its homozygous state the nor gene totally inhibited fruit ripening. However, as a heterozygote it revealed an intermediate action, slowing down fruit ripening and prolonging shelf life 2-3 times as compared with standard tomato. The expression of the nor gene was also found to be dependent on the genetic background. Line C36 positively influenced the flavour and firmness of hybrid fruits, while Dw₄ had a negative effect on fruit quality.
Źródło:
Journal of Applied Genetics; 1998, 39, 3; 259-273
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nowy mutant rzepaku ozimego o podwyzszonej zawartosci kwasow palmitynowego i stearynowego w oleju nasion
Autorzy:
Spasibionek, S
Powiązania:
https://bibliotekanauki.pl/articles/834173.pdf
Data publikacji:
2003
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
mutanty
rosliny oleiste
nasiona
kwas palmitynowy
zawartosc oleju
kwas stearynowy
rzepak ozimy
mutant
oil plant
seed
palmitic acid
oil content
stearic acid
winter rape
Źródło:
Rośliny Oleiste - Oilseed Crops; 2003, 24, 2; 671-680
1233-8273
Pojawia się w:
Rośliny Oleiste - Oilseed Crops
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Izolacja i wstepna charakterystyka niskoproteazowych mutantow Trichoderma reesei
Autorzy:
Janas, P
Fiedurek, J
Targonski, Z
Mleko, S
Powiązania:
https://bibliotekanauki.pl/articles/828717.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Technologów Żywności
Tematy:
mutanty
aktywnosc proteolityczna
grzyby
aktywnosc enzymatyczna
Trichoderma reesei
enzymy celulolityczne
biomasa
serwatka
laktoza
mutant
proteolytic activity
fungi
enzyme activity
cellulolytic enzyme
biomass
whey
lactose
Opis:
Po mutagenizacji promieniowaniem UV i NTG szczepu Trichoderma reesei M-7 i selekcji na podłożu z dodatkiem żelatyny wyizolowano około 960 niskoproteazowych mutantów. Dziesięć z nich zostało przetestowanych podczas hodowli okresowych w zakresie produkcji celulaz, proteaz i beta-galaktozydazy w obecności serwatki i laktozy jako źródła węgla. Aktywności proteolityczne oznaczone po hodowlach 9 mutantów w obecności laktozy były niższe od szczepu wyjściowego. Zaobserwowano większy indukcyjny wpływ serwatki niż laktozy, na sekrecję proteaz przez wszystkie badane szczepy. Czysta laktoza okazała się bardziej efektywnym induktorem produkcji beta-galaktozydazy niż zawarta w serwatce. Plony biomasy większości mutantów po hodowlach na serwatce były tylko nieznacznie wyższe od oznaczonych po hodowlach na czystej laktozie. Było to prawdopodobnie związane z litycznym mechanizmem kompensującym proteaz, przejawiających wyższe aktywności w filtratach pochodzących z hodowli w obecności serwatki. Praca jest wstępem do dalszych badań nad zależnościami pomiędzy proteazami i innymi enzymami w filtratach pohodowlanych Trichoderma reesei.
It has been isolated 960 low-protease mutants after mutagenisation using UV radiation and NTG and selection on medium with addition of gellatine. Ten of them have been tested during batch cultivation for the production of cellulases, proteases and beta-galactosidase in the presence of whey and lactose as a source of carbon. Proteolytic activities estimated after cultivation of 9 mutants in the presence of lactose were lower then parental strain. It has been observed better inductive effect of whey then lactose on the secretion of proteases by all mutants tested. Pure lactose appeared to be more effective inducer of the production of beta-galactosidase then contains in whey. Biomass concentration of most mutants after cultivation on whey was only slightly higher then estimated after cultivation on pure lactose. It was probably connected with lytic compensatory mechanism of proteases exhibited higher activities in culture filtrates from cultivation in the presence of whey. This work is an introduction for future studies on correlation between proteases and other enzymes in culture filtrates of Trichoderma reesei.
Źródło:
Żywność Nauka Technologia Jakość; 2003, 10, 2; 17-26
1425-6959
Pojawia się w:
Żywność Nauka Technologia Jakość
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Morphological and physiological characterization of the delta-psaL mutant of cyanobacterium Synechocystis PCC6803
Autorzy:
Klodawska, K.
Kis, M.
Malec, P.
Kovacs, L.
Gombos, Z.
Strzalka, K.
Powiązania:
https://bibliotekanauki.pl/articles/81106.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
Cyanoprokaryota
prokaryotic organism
photosynthesis
photosystem
thylakoid membrane
plastoquinone
plastocyanin
cytochrome C
biotic stress
abiotic stress
Synechocystis
delta-psaL mutant
physiological characteristics
morphological characteristics
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Indukowanie zmiennosci cech jakosciowych oleju gorczycy bialej [Sinapis alba L.]
Autorzy:
Olejniczak, J
Adamska, E.
Powiązania:
https://bibliotekanauki.pl/articles/833002.pdf
Data publikacji:
2000
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
mutanty
gorczyca biala
zmiennosc cech
tokoferole
genetyka roslin
kwasy tluszczowe
oleje
cechy jakosciowe
mutant
white mustard
trait variability
tocopherol
plant genetics
fatty acid
oil
quality trait
Źródło:
Rośliny Oleiste - Oilseed Crops; 2000, 21, 2; 641-647
1233-8273
Pojawia się w:
Rośliny Oleiste - Oilseed Crops
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mutanty rzepaku ozimego podwojnie ulepszonego o zmienionym skladzie kwasow tluszczowych.
Autorzy:
Spasibionek, S
Byczynska, B.
Krzymanski, J.
Powiązania:
https://bibliotekanauki.pl/articles/833704.pdf
Data publikacji:
2000
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
mutanty
kwas oleinowy
kwasy tluszczowe
kwas linolenowy
kwas linolowy
rzepak dwuzerowy
EMS
rzepak ozimy
desaturaza
mutant
oleic acid
fatty acid
linolenic acid
linoleic acid
double-improved cultivar
rape
ethyl methanesulphonate
desaturase
Źródło:
Rośliny Oleiste - Oilseed Crops; 2000, 21, 3; 715-724
1233-8273
Pojawia się w:
Rośliny Oleiste - Oilseed Crops
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena ploidalnosci mutantów chryzantemy wielkokwiatowej (Dendranthema grandiflora Tzvelev) uzyskanych w wyniku mutagenezy indukowanej in vitro i in vivo promieniowaniem jonizującym
Evaluation of ploidy in chrysanthemum mutants [Dendranthema grandiflora Tzvelev] obtained in mutagenesis induced in vitro and in vivo by ionizing radiation
Autorzy:
Lema-Rumińska, J.
Zalewska, M.
Powiązania:
https://bibliotekanauki.pl/articles/11347078.pdf
Data publikacji:
2002
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
mutageneza indukowana in vivo
mutanty
Dendranthema grandiflora
mutageneza indukowana in vitro
chryzantema wielkokwiatowa
chromosomy
promieniowanie jonizujace
ploidalnosc
chromosome
in vitro mutagenesis
in vivo mutagenesis
ionizing radiation
mother chrysanthemum
mutant
ploidy
Opis:
Badano liczbę chromosomów w komórkach merystemów wierzchołkowych korzeni trzech odmian wyjściowych chryzantemy wielkokwiatowej (Dendranthema grandiflora Tzvelev) i odmian pochodnych, otrzymanych w wyniku radiomutacji. Wykazano, że tylko jedna odmiana mutacyjna – ‘Bronze Wonder’ miała inną liczb chromosomów niż odmiana wyjściowa ‘Lilac Wonder’.
The present research investigated the number of chromosomes in the cells of apical meristems of roots for three original Dendranthema grandiflora Tzvelev cultivars and the derivative cultivars obtained due to radiomutation. It was shown that only in one mutation cultivar, ‘Bronze Wonder’, the number of chromosomes differed from that of the original cultivar, ‘Lilac Wonder’.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2002, 01, 2; 43-48
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Somaclonal selection of physiological mutants and several problems related to rice cell breeding
Autorzy:
Kinoshita, T
Mori, K.
Powiązania:
https://bibliotekanauki.pl/articles/2044460.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
chromosome variation
japonica rice
Oryza
African rice
physiological mutant
gamma-radiation
anther culture
hybrid plant
protoplast isolation
callus induction
callus formation
aneuploidy
indica rice
herbicide tolerance
hybrid
Kitaake cultivar
gametoclonal variation
somatic hybrid
rice
Opis:
Tolerance to a new herbicide, pyributycarb, was evaluated both at the plant and cellular levels. Several highly or moderately tolerant strains chosen at the plant level, showed a parallel relation of to tolerance at the cellular level. However, on the whole, correlation between total tolerance indices and survival rates of calli was not significant in 18 out of the 80 studied strains. As a result of somaclonal selection for two herbicides, lines NB-200 and NM-100 were regenerated from the tolerant calli screened with benthiocarb at 200 ppm, and molinate at 100 ppm, respectively. In the R₂ generation, both the lines displaned a stable tolerance both at the plant and cellular levels. Thus the highly tolerant mutant lines were developed from a moderately tolerant line, N-61, via in vitro selection. To achieve a short-cut method in the interspecific genetic exchange, a series of techniques related to cell fusion were established in rice and related species. Two kinds of somatic hybrids between the cultivar Kitaake and tetraploid Oryza species, O. punctata and O. officinalis, were successfully produced. Among the somatic hybrid plants, a wide range of chromosomal variation was observed. Aneuploid plants with a chromosome number around 2n = 72 (hexaploid), which are expected from a symmetric fusion between diploid and tetraploid strains, were obtained showing mixoploidy within a plant. Most of the somatic hybrids were characterized by intermediate features of plant-type showing high sterility, shattering of spikelets and reduced plant height. As an exception, a diploid plant, which was identified by RFLP analysis using the rDNA gene probe, closely resembled Kitaake and produced viable seeds. A tetraploid hybrid plant was also promising for the introduction of economically important characters through the reduction of chromosome numbers by doubled haploids. Gametoclonal variation and gamma radiation was applied to Kitaake. The mutation frequency was prominently increased by gamma ray treatment, especially at high doses of 200 Gy or 300 Gy. In the M₃R₂ or M₄R₃ generations, most of the variants showed unfavourable characters. Most of the mutant characters were governed by single or double recessive genes. Several mutants such as short culm and early flowering time might be used for rice breeding.
Źródło:
Journal of Applied Genetics; 1998, 39, 1; 9-35
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cechy mutantow rzepaku ozimego o zmienionym skladzie kwasow tluszczowych
Autorzy:
Spasibionek, S
Powiązania:
https://bibliotekanauki.pl/articles/834297.pdf
Data publikacji:
2004
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
mutanty
kwas oleinowy
rosliny oleiste
cechy genetyczne
nasiona
mutageneza chemiczna
kwasy tluszczowe
kwas linolenowy
kwas linolowy
rzepak ozimy
mutant
oleic acid
oil plant
genetic trait
seed
chemical mutagenesis
fatty acid
linolenic acid
linoleic acid
winter rape
Źródło:
Rośliny Oleiste - Oilseed Crops; 2004, 25, 2; 383-401
1233-8273
Pojawia się w:
Rośliny Oleiste - Oilseed Crops
Dostawca treści:
Biblioteka Nauki
Artykuł
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