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Wyszukujesz frazę "molecular typing" wg kryterium: Temat


Wyświetlanie 1-3 z 3
Tytuł:
Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis
Autorzy:
Karakulska, J.
Pobucewicz, A.
Nawrotek, P.
Muszynska, M.
Furowicz, A.J.
Czernomysy-Furowicz, D.
Powiązania:
https://bibliotekanauki.pl/articles/31963.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
molecular typing
Staphylococcus aureus
PCR-RFLP method
coa gene
random amplified polymorphic DNA analysis
mastitis
nuc gene
gap gene
milk sample
species identification
Opis:
The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Patogenne lęgniowce z rodzaju Phytophthora - nowe zagrożenie lasów w Europie
Pathogenic oomycetes of Phytophthora genus - a new threat to forests in Europe
Autorzy:
Ajchler, M.
Łobocka, M.
Oszako, T.
Powiązania:
https://bibliotekanauki.pl/articles/986789.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
fitopatologia lesna
zagrozenia drzewostanow
czynniki chorobotworcze
legniowce
Oomycetes
Phytophthora
fytoftoroza
identyfikacja
epidemiologia
Phytophthora alni
Phytophthora cactorum
Phytophthora cambivora
Phytophthora cinnamomi
Phytophthora citrophthora
Phytophthora quercina
Phytophthora plurivora
Phytophthora ramorum
ochrona lasu
zwalczanie chorob roslin
oomycetes
phytophthora
molecular typing
plant pathogens
biocontrol
Opis:
Pathogenic oomycetes represented mainly by the species of Phytophthora genus are among the most dangerous plant pathogens. They pose a serious threat for agricultural as well as wild plants, and are involved in forest decline worldwide. Over 140 pathogenic Phytophthora species have been identified so far. The common infection symptoms include rotting of below− and above− ground parts of plants, causing weakness and slow decline of infected trees. The economic losses caused by certain Phytophthora species may rich even 100%. Globalization and border opening have facilitated the transport of plant material between countries and continents, thus increased the risk of transfer of various Phytophthora genus representatives to new geographical locations. Global warming (e.g. mild winters) have facilitated the expansion of species from southern to northern Europe. Among Polish Phytophthora isolates are species that have previously been known only in nurseries (e.g. P. cactorum), but nowadays they are also isolated from forests (e.g. oak stand in the Krotoszyn Plateau). It suggests the pathway from nurseries to stands with plant for plantings and attached soil. There are also new species, that have not been isolated so far in the world (P. polonica) or found far away from Poland (P. fragaliaefolia in Japan on strawberry). The possible natural pathways are birds and water courses. In Mazowsze and Wielkopolska regions (C and W Poland) the polyphagous P. cinnamomi was found on pedunculate oaks (Quercus robur). This species causes significant damage to red oak forest in France, but also threats Jarrah forests in the Australian ecosystem (it attacks ca. 1000 species of plants). Fortunately, in addition to time consuming and laborious classic methods of Phytophthora identification, based on morphology and physiological properties, molecular methods that are based on immunological tests and chromosomal or mito− chondrial DNA markers identification have come into common use. Despite morphological similarity to true fungi, oomycetes are more closely related to diatoms and brown algae, and have several structural features that differentiate them from fungi, including the cell wall composed of cellulose instead of chitin. That is one of the reasons that fungicides have a limited use in the fight with Phytophthora infections. Additionally, type of ecological niches that are settled by pathogenic oomycetes (root remnants in soil, watercourses) hinders the chemical combating. Biocontrol, i.e. the use of interspecies interactions between microorganisms (bacteria, fungi) to limit the growth and development of pathogens, seems to be a reasonable alternative.
Źródło:
Sylwan; 2017, 161, 10; 870-880
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular identification of Trichoderma strains collected to develop plant growth-promoting and biocontrol agents
Autorzy:
Oskiera, M.
Szczech, M.
Bartoszewski, G.
Powiązania:
https://bibliotekanauki.pl/articles/1976.pdf
Data publikacji:
2015
Wydawca:
Instytut Ogrodnictwa
Tematy:
Trichoderma
strain
molecular identification
biological control agent
multilocus sequence typing
phylogenesis
species identification
DNA isolation
Źródło:
Journal of Horticultural Research; 2015, 23, 1
2300-5009
Pojawia się w:
Journal of Horticultural Research
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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