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Wyświetlanie 1-10 z 13
Tytuł:
Molecular detection of Anaplasmataceae in blood samples from dogs
Autorzy:
Adamczyk, M.
Nowak, Z.
Gajda, E.
Bunkowska-Gawlik, K.
Perec-Matysiak, A.
Janaczyk, B.
Hildebrand, J.
Powiązania:
https://bibliotekanauki.pl/articles/6250.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
molecular detection
Anaplasmataceae
blood sample
dog
animal disease
vector-borne disease
Anaplasma phagocytophilum
Neoehrlichia mikurensis
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Serological and molecular detection of bean leaf roll and chickpea chlorotic stunt luteoviruses in chickpea from Iran
Autorzy:
Hajiyusef, T.
Shahraeen, N.
Maleki, M.
Powiązania:
https://bibliotekanauki.pl/articles/66046.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bean leafroll virus
serological detection
molecular detection
chick-pea chlorotic stunt luteovirus
chick-pea
Cicer arietinum
phylogenesis
serology
Iran
Opis:
Chickpea (Cicer arietinum L.) is an important legume crop and widely cultivated in northwestern provinces of Iran. During a survey in the 2015 growing season a total of 170 selected chickpea plants with general yellowing symptoms including stunting and leaf bronzing were collected. Serological Elisa and tissue blot immunoassay (TIBA) tests revealed the presence of Bean leaf roll virus (BLRV) and Chickpea chlorotic stunt virus (CpCSV) as the predominant viruses in the region. Some serologically positive samples of BLRV and CpCSV were selected and rechecked by RT-PCR. Th e results of amplifi ed PCR products using a specifi c pair of primers towards the Cp gene region of the viruses were approximately 413 bp for CpCSV and 391 bp for BLRV. Results obtained from sequence comparison of BLRV (IR-F-Lor-5) isolate form two subgroups with eight other BLRV isolates from GeneBank indicating a high homology of 96% with isolates from Argentina, Germany, Tunisia, USA, Spain, and Colombia. An isolate from Norabad (Iran) (IR-Nor) had 98% homology with HQ840727 Libyan isolate. CpCSV sequence comparison with six other GeneBank isolates indicated 98% homology with isolates from Tunisia and Azerbaijan. Th e overall results of this research revealed the CpCSV and BLRV (luteoviruses) associated with the yellowing disease syndrome of chickpea crops in the surveyed region.
Źródło:
Journal of Plant Protection Research; 2017, 57, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Recreational and drinking waters as a source of norovirus gastroenteritis outbreaks: a review and update
Autorzy:
La Rosa, G.
Pourshaban, M.
Iaconelli, M.
Muscillo, M.
Powiązania:
https://bibliotekanauki.pl/articles/363240.pdf
Data publikacji:
2008
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
woda pitna
detekcja molekularna
norowirus
wybuch
woda rekreacyjna
drinking water
molecular detection
norovirus
outbreak
recreational water
Opis:
The distribution of noroviruses is worldwide. In industrialized countries, noroviruses are the most common viral cause of gastroenteritis outbreaks and play an important role in sporadic gastroenteritis as well. Transmission may occur through the ingestion of contaminated foods or water, through person-to-person contact, or by way of direct contact with contaminated surfaces. Of particular importance is their ability to cause waterborne outbreaks linked either to the direct consumption of water or to its recreational uses. This article reviews the clinical manifestations and epidemiology of norovirus infection, and describes over 40 waterborne norovirus outbreaks, their respective probable sources of contamination and - where water samples were tested - the genetic types identified.
Źródło:
Environmental Biotechnology; 2008, 4, 1; 15-24
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular detection of Bartonella sp. in carnivores in Poland
Autorzy:
Szewczyk, T.
Karbowiak, G.
Werszko, J.
Powiązania:
https://bibliotekanauki.pl/articles/5816.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
molecular detection
bacteria
Bartonella
Bartonellaceae
carnivore
cat scratch disease zob.bartonellosis
cat scratch fever zob.bartonellosis
bartonellosis
Polska
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular detection of Ehrlichia canis, Hepatozoon canis and Babesia canis vogeli in stray dogs in Mahasarakham province, Thailand
Autorzy:
Piratae, S.
Pimpjong, K.
Vaisusuk, K.
Chatan, W.
Powiązania:
https://bibliotekanauki.pl/articles/5810.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
molecular detection
Ehrlichia canis
Hepatozoon canis
Babesia canis vogeli
stray dog
dog
tick-borne pathogen
Mahasarakham province
Thailand
Opis:
Canine tick borne diseases showing distribution worldwide have caused morbidity and mortality in dogs. This study observed the mainly tick borne pathogens described for dogs in Thailand, Ehrlichia canis, Hepatozoon canis and Babesia canis vogeli. From May to July 2014, blood samples were collected from 79 stray dogs from 7 districts of Mahasarakham province to molecular surveyed for 16s rRNA gene of E. canis and 18s rRNA gene of H. canis and B. canis vogeli. Twenty eight (35.44%) of stray dogs showed the infection with tick borne pathogens. The prevalence of E. canis infection was the highest with 21.5% (17/79). DNA of H. canis and B. canis vogeli were detected at the prevalence of 10.1% (8/79) and 6.3% (5/79), respectively. Co-infection between E. canis and B. canis vogeli were identified in 2 (2.5%) dogs. The results indicated that a wide range of tick borne pathogens are circulation in the canine population in Mahasarakham province. This study is the first report on prevalence of E. canis, H. canis and B. canis vogeli in stray dogs in Mahasarakham, a province in northern part of Thailand. This data providing is important to understand the prevalence of E. canis, H. canis and B. canis vogeli infection in stray dogs in this region, which will assist in the management of these blood parasite.
Źródło:
Annals of Parasitology; 2015, 61, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biotechnology of temperate fruit trees and grapevines
Autorzy:
Laimer, Margit
Mendonça, Duarte
Maghuly, Fatemeh
Marzban, Gorji
Leopold, Stephan
Khan, Mahmood
Balla, Ildiko
Katinger, Hermann
Powiązania:
https://bibliotekanauki.pl/articles/1041374.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
plant biotechnology
patogen detection
molecular markers
Opis:
Challenges concerning fruit trees and grapevines as long lived woody perennial crops require adapted biotechnological approaches, if solutions are to be found within a reasonable time frame. These challenges are represented by the need for correct identification of genetic resources, with the foreseen use either in conservation or in breeding programmes. Molecular markers provide most accurate information and will be the major solution for questions about plant breeders rights. Providing healthy planting material and rapid detection of newly introduced pathogens by reliable methods involving serological and molecular biological tools will be a future challenge of increases importance, given the fact that plant material travels freely in the entire European Union. But also new breeding goals and transgenic solutions are part of the biotechnological benefits, e.g. resistance against biotic and abiotic stress factors, modified growth habits, modified nutritional properties and altered processing and storage qualities. The successful characterization of transgenic grapevines and stone fruit trees carrying genes of viral origin in different vectors constructed under ecological consideration, will be presented. Beyond technical feasibility, efficiency of resistance, environmental safety and Intellectual Property Rights, also public acceptance needs consideration and has been addressed in a specific project. The molecular determination of internal quality parameters of food can also be addressed by the use of biotechnological tools. Patient independent detection tools for apple allergens have been developed and should allow to compare fruits from different production systems, sites, and genotypes for their content of health threatening compounds.
Źródło:
Acta Biochimica Polonica; 2005, 52, 3; 673-678
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molekularne sondy fluorescencyjne w biologii, biochemii i biotechnologii
Molecular fluorescent probes in biology, biochemistry and biotechnology
Autorzy:
Kukuła, K.
Kamińska, I.
Ortyl, J.
Chachaj-Brekiesz, A.
Powiązania:
https://bibliotekanauki.pl/articles/134758.pdf
Data publikacji:
2015
Wydawca:
ADVSEO
Tematy:
sondy fluorescencyjne
biologia molekularna
wykrywanie białek
fluorescent probes
molecular biology
detection of proteins
Opis:
Nowadays molecular fluorescent probes are widely used in many fields of science, primarily in biology, medicine and biotechnology. Particular emphasis on the development of technologies to cancer cells diagnosis and detection of genetic mutations led to numerous modification of luminescent fluorophores, which has resulted in a wide range of such compounds. Scientists from around the world are continuously trying to experience the mechanism of cells function and metabolic pathways. Certainly, it is affected by the fact that any analytical techniques using fluorescence operate easily and nondestructively. A phenomenon of fluorescence is observable by chromophore which absorbs energy at a certain wavelength and is able to emit electromagnetic radiation at another wavelength.
Źródło:
Technical Issues; 2015, 4; 19-25
2392-3954
Pojawia się w:
Technical Issues
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection and molecular characterization of the Iris severe mosaic virus-Ir isolate from Iran
Autorzy:
Nateqi, M.
Habibi, M.K.
Dizadji, A.
Parizad, S.
Powiązania:
https://bibliotekanauki.pl/articles/66139.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
detection
molecular characteristics
iris severe mosaic virus
isolate
phylogenesis
iris
Iridaceae
ornamental plant
Iran
Opis:
Iris belongs to the Iridaceae family. It is one of the most important pharmaceutical and ornamental plants in the world. To assess the potyvirus incidence in natural resources of iris plants in Iran, Antigen Coated-Plate ELISA (ACP-ELISA) was performed on 490 symptomatic rhizomatous iris leaf samples, which detected the potyvirus in 36.7% of the samples. Genomic 3’ end of one mechanically non-transmitted potyvirus isolate, comprising a 3’ untranslated region (390 bp) and C-terminus of the coat protein (CP) gene (459 bp), was amplified by reverse transcription polymerase chain reaction (RT-PCR), which was ligated into pTG19-T vector. The nucleotide sequence of amplicons was compared with related sequences, using Blastn software available at NCBI GenBank, and showed the highest similarity with Iris severe mosaic virus (ISMV) isolates. The nucleotide and deduced amino acid sequence of the CP C-terminus region was more than 83% identical with other ISMV isolates, therefore this isolate was designated as ISMV-Ir. This new ISMV isolate is closely related to the Chinese ISMV-PHz in phylogenetic analysis, based on the partial nucleotide and deduced amino acid sequence of the CP region. This is the first report of ISMV occurrence on Iris spp. in Iran.
Źródło:
Journal of Plant Protection Research; 2015, 55, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Progress in the molecular methods for the detection and genetic characterization of Cryptosporidium in water samples
Autorzy:
Skotarczak, B
Powiązania:
https://bibliotekanauki.pl/articles/52184.pdf
Data publikacji:
2010
Wydawca:
Instytut Medycyny Wsi
Tematy:
protozoan parasite
Cryptosporidium
genetic characteristics
molecular method
detection
water sample
food-borne way
oocyst
infection control
Źródło:
Annals of Agricultural and Environmental Medicine; 2010, 17, 1; 1-8
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
PCR-RFLP method to distinguish Frankliniella occidentalis, Frankliniella intonsa, Frankliniella pallida and Frankliniella tenuicornis
Autorzy:
Przybylska, A.
Fiedler, Z.
Obrepalska-Steplowska, A.
Powiązania:
https://bibliotekanauki.pl/articles/66435.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
PCR-RFLP method
Frankliniella occidentalis
Frankliniella intonsa
Frankliniella pallida
Frankliniella tenuicornis
thrip
molecular diagnostics
pest control
detection
Opis:
Thrips from the genus Frankliniella (Thysanoptera, Thripidae) are phytophagous on crops and wild plants. Some of them cause slight economic damage, however, others including F. occidentalis and F. intonsa are responsible for considerable losses in crop production. Moreover, they constitute a double threat for host plants by not only feeding on them but also vectoring viruses, some of which are on the quarantined list of the European Plant Protection Organization. The rapid detection and differentiation between more and less harmful Frankliniella species is, therefore, important in order to combat the pests at the time of their appearance. In this study, we have undertaken to develop a method of detecting F. occidentalis, F. intonsa, F. pallida, and F. tenuicornis. The protocol is based on PCR amplification of ITS1 rDNA fragments of these insects using universal primers pair giving products of slightly distinct length for studied insects. Restriction enzymes digestion which is easy to interpret, allows for visible differentiation of all these Frankliniella species. The method was shown to be species-specific and sensitive. Even single specimens in either the larvae or adult stage could be distinguished.
Źródło:
Journal of Plant Protection Research; 2016, 56, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
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