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Wyszukujesz frazę "microsatellite DNA" wg kryterium: Temat


Wyświetlanie 1-11 z 11
Tytuł:
Monitoring of cellular chimerism in patients after sex-mismatched bone marrow transplantation: technical report
Autorzy:
Jolkowska, J
Ladon, D.
Wachowiak, J.
Witt, M.
Powiązania:
https://bibliotekanauki.pl/articles/2043435.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
in situ
allogeneic transplantation
chimerism
molecular analysis
transplantation
minimal residual disease
acute myeloblastic leukemia
polymorphic microsatellite
bone marrow
hybridization
polymerase chain reaction
DNA
detection
Źródło:
Journal of Applied Genetics; 2000, 41, 3; 209-212
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of microsatellite instability and loss of heterozygosity in breast cancer with the use of a well characterized multiplex system.
Autorzy:
Powierska-Czarny, Jolanta
Miścicka-Śliwka, Danuta
Czarny, Jakub
Grzybowski, Tomasz
Woźniak, Marcin
Drewa, Gerard
Czechowicz, Włodzimierz
Sir, Jan
Powiązania:
https://bibliotekanauki.pl/articles/1043412.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
microsatellite instability
short tandem repeats
multiplex DNA typing
breast cancer
Opis:
Analysis of microsatellite instability (MI) and loss of heterozygosity (LOH) is recommended for screening patients with sporadic and hereditary malignancies. This study shows an application of a fluorescent hexaplex PCR system for microsatellite typing on A.L.F. DNA Sequencer (Pharmacia Biotech). This technique detects changes in microsatellites providing a time-efficient, reliable and accurate method for MI and LOH analyses. The Fragment Manager software was used for automated size calculation and quantitation of DNA fragments, enabling rapid and precise measurement of allelic ratios. We examined 70 breast cancer and 70 control DNA specimens, classified all the patterns of microsatellite alterations, and set up MI and LOH assessment criteria for the automated multiplex fluorescent method.
Źródło:
Acta Biochimica Polonica; 2003, 50, 4; 1195-1203
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microsatellite DNA polymorphism in sturgeon species and their hybrids reared in Polish aquaculture farms
Autorzy:
Fopp-Bayat, D.
Luczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/363194.pdf
Data publikacji:
2006
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
jesiotr
identyfikacja gatunkowa
DNA mikrosatelitarne
hybrydy
polimorfizm
hybrids
microsatellite DNA
polymorphism
PCR
species identification
sturgeons
Opis:
Highly variable microsatellite DNA loci show high levels of variation per locus and provide molecular markers for species and for populations of small effective size. In this study we applied microsatellite markers to identify specimens belonging to several sturgeon species and their interspecific hybrids. Nine microsatellite loci (Afu-19, Afu-22, Afu-34, Afu-39, Afu-54, Afu-57, Afu-58, Afu-68, Afu-69) were analysed for five species (beluga, Siberian sturgeon, Russian sturgeon, sterlet and paddlefish) and for their three interspecific hybrids: Siberian sturgeon x Russian sturgeon, beluga x sterlet (called bester) and for beluga x bester. Certain alleles at five loci: Afu-22, Afu-39, Afu-54, Afu-57 and Afu-68 were diagnostic for the examined sturgeon species and their interspecific hybrids.
Źródło:
Environmental Biotechnology; 2006, 2, 1; 11-19
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic identification of black caviar based on microsatellite DNA analysis
Autorzy:
Fopp-Bayat, D.
Powiązania:
https://bibliotekanauki.pl/articles/363094.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
identyfikacja genetyczna
czarny kawior
DNA mikrosatelitarne
genetic identification
black caviar
microsatellite DNA
Opis:
Sturgeons (Acipenseridae) are producers of black caviar, which is sold world-wide. Black caviar differs widely in quality, availability, price and taste and for these reasons it is often the subject of commercial fraud. Identification of sturgeon species is frequently based on molecular methods such as analysis of nuclear DNA and mitochondrial DNA. In this study two fragments of microsatellite DNA: Afu-39 and Afu-68 were analyzed. In caviar samples, four alleles were observed in locus Afu-39, and six alleles in locus Afu-68. This paper describes the use of microsatellite DNA markers for species identification of black caviar.
Źródło:
Environmental Biotechnology; 2007, 3, 2; 57-60
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polymorphism of microsatellite loci - a tool in studying biodiversity of paddlefish aquaculture broodstock
Autorzy:
Kaczmarczyk, D.
Kohlmann, K.
Kersten, P.
Luczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/363088.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
izolacja DNA
DNA mikrosatelitarne
amplifikacja PCR
DNA extraction
microsatellite DNA
PCR amplification
Opis:
American paddlefish (Polyodon spathula) is a new species in Polish aquaculture, its broodstocks are few and small, and it is possible that all mature fish originated from only a few spawners. Studies on polymorphism of highly variable microsatellite DNA allow revealing genetic characteristics of individual spawners as well as estimation of genetic variation within and divergence between broodstocks. This paper describes optimised protocols for isolation of DNA from fin tissues, amplification of nine microsatellite loci using PCR technique, and for fish genotyping using automatic capillary DNA sequencer. Our technique was tested towards the fin samples taken from all paddlefish reared in Poland and approaching their sexual maturity; the study included also samples taken from 47 fish of the Ukrainian breeding center (Gorny Tykich).
Źródło:
Environmental Biotechnology; 2007, 3, 2; 44-48
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mitochondrial and nuclear DNA differentiation of Picea abies populations in Poland
Autorzy:
Nowakowska, J A
Powiązania:
https://bibliotekanauki.pl/articles/40976.pdf
Data publikacji:
2009
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
international conference
Europe
forest ecosystem
plant breeding
tree
Norway spruce
Picea abies
Polska
mitochondrial DNA
nuclear DNA
plant population
microsatellite marker
post-glacial migration
Opis:
The natural stands of Norway spruce in Poland are split between the southern and the northeastern parts of the country. Two so-called "spruceless" zones separate the northern spruce locations from those in the south, one "spruceless" zone is situated in Central Poland, and the other one in the Beskid Mts. Mitochondrial (STS) and nuclear (SSR) markers were used to perform the genetic identification of Norway spruce. Four different variants of haplotypes, "a", "b", "c" and "d", were found to occur in the nad1 locus of STS markers. Populations from the northern range of Picea abies distribution in Poland harboured exclusively haplotypes "c" and "d", except for the Białowieża population which had haplotypes "a" and "c". Populations from the "spruceless" zones contained four types of haplotypes whilst those from southern Poland were mostly composed of haplotype "a". High mean gene diversity was observed for both STS and SSR markers (HT = 0.529, and HT = 0.851, respectively). The total genetic differentiation of Norway spruce populations was very low (FST= 0.088). Two main groups of populations were distinguished in the dendrogram defined by Nei's genetic distances based on microsatellite markers. The distribution of the genotypes was scattered and did not show any connection with the spatial distribution of P. abies in Poland. Only the mtDNA markers were able to differentiate the northern populations of Norway spruce from the southern ones, proving the historical separation between the Baltico-Nordic and the Hercyno-Carpathian ranges of P. abies in Poland. By contrast, the microsatellite data suggested an overlap between the genotypes due to the human manipulation of Norway spruce stands in the past.
Źródło:
Dendrobiology; 2009, 61 Supplement
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Tracing the genetic origin of brown trout (Salmo trutta) re-colonizing the Ecker reservoir in the Hartz National Park, Germany
Autorzy:
Kohlmann, K.
Wüstemann, O.
Powiązania:
https://bibliotekanauki.pl/articles/363212.pdf
Data publikacji:
2012
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
pstrąg potokowy
Salmo trutta
mikrosatelitarne DNA
DNA
PCR
brown trout
microsatellite
Opis:
The Ecker reservoir and its main tributary had been free of brown trout (Salmo trutta) for several decades due to cumulative effects of natural and anthropogenic acidification. However, after the decline of emissions in the 1990s and the resulting rise of water pH to suitable for brown trout values, the species began to recolonize its original habitats. In the main tributary first brown trout individuals were caught in 2008 and in the reservoir in later years as well. Stocking could be excluded in both areas. Therefore, the present study was aimed to trace the genetic origin of these brown trout by genotyping eight microsatellite loci in samples collected in the reservoir, its main tributary, potential refugia and - for comparison - from two areas downstream of the dam being physically isolated for about 70 years. Genetic variability within populations (average number of alleles per locus), genetic differentiation between populations (FST values and genetic distances), occurrence of certain alleles and results of assignment tests indicated that the Ecker reservoir was re-colonized from two sources: the Große Peseke, a small direct inflow into the reservoir, and the Fuhler Lohnsbach, a parallel flowing brook connected to the reservoir by a pipe. Genetic data also supported recolonization of the main tributary from the reservoir but not in the opposite direction. Moreover, bottleneck effects were evident in brown trout populations upstream of the dam compared to the two populations downstream of the dam.
Źródło:
Environmental Biotechnology; 2012, 8, 2; 39-44
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New multiplex PCR assays for estimating genetic diversity in rainbow trout (Oncorhynchus mykiss) by polymorphism of microsatellite DNA
Autorzy:
Kaczmarczyk, D.
Kaczor, A.
Powiązania:
https://bibliotekanauki.pl/articles/363262.pdf
Data publikacji:
2013
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
microsatellite DNA
multiplex PCR
population genetics
rainbow trout
DNA mikrosatelitarne
genetyka populacyjna
pstrąg tęczowy
Opis:
Multiplex PCR is a useful technique for estimating genetic diversity. This paper presents 3 new sets of primer pairs for effectively amplifying 10 microsatellite DNA loci from rainbow trout (Oncorhynchus mykiss). Unlike other sets of primer pairs that have been developed for amplifying rainbow trout microsatellite loci, ours do not require the hot-start PCR technique. In the paper, we describe the steps taken to choose the loci for each multiplex assay and to verify the genotyping results. We provide the compositions of the PCR mixture and the characteristics of the PCR thermal profile recommended for amplification.
Źródło:
Environmental Biotechnology; 2013, 9, 1; 19-24
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Czy na plantacjach nasiennych zawężamy zmienność genetyczną? Próba odpowiedzi na podstawie analiz mikrosatelitarnego DNA szczepów rosnących na plantacji nasiennej sosny zwyczajnej (Pinus sylvestris L.) z Nadleśnictwa Susz
Are we narrowing genetic variability in seed orchards? An attempt to answer, based on the analysis of microsatellite DNA of grafts growing in Scots pine (Pinus sylvestris L.) seed orchard in the Forest District Susz
Autorzy:
Przybylski, P.
Powiązania:
https://bibliotekanauki.pl/articles/1311426.pdf
Data publikacji:
2015
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
lesnictwo
hodowla lasu
plantacje nasienne
sosna zwyczajna
Pinus sylvestris
zmiennosc genetyczna
DNA mikrosatelitarny
analiza DNA
microsatellite DNA
tree breeding
seed orchard
Opis:
Scots pine (Pinus sylvestris L.) is the most common species in Poland’s forest stands. The mode of pine stands renovation requires that silviculture practitioners have continuous access to seed banks. Orchard-grown seeds are predicted to constitute an increasingly larger part of the average demand for pine seeds in Poland. Seed orchards, due to a limited number of maternal trees as well as the irregularity of their blooming and pollination, enhance the risk of genetic diversity reduction in planted forest stands. This is of particular importance in the context of dynamic climate change. Markers based on microsatellite DNA fragments are effective tools for monitoring genetic variability. In the present study, three different microsatellite DNA fragments were used: SPAC 12.5, SPAG 7.14 and SPAC 11.4. The main objective of this research was to study genetic variability in one of the biggest seed orchards in Poland, located in the Forest District Susz. The obtained results indicated heterozygosity loss within the orchard, proving the existence of specimen selection effects on genetic variability. Hence, it seems quite important to take account of molecular genetic variability of maternal trees in future breeding strategies.
Źródło:
Leśne Prace Badawcze; 2015, 76, 3; 240-249
1732-9442
2082-8926
Pojawia się w:
Leśne Prace Badawcze
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zmienność taborskiej sosny zwyczajnej (Pinus sylvestris L.) z Nadleśnictwa Miłomłyn wyrażona w analizie cech morfologii igieł oraz polimorfizmie mikrosatelitarnego DNA
Variability of Scots pine (Pinus sylvestris L.) called Taborz pine (Forest District Milomlyn) expressed in analysis of morphology of needle traits and polymorphism of microsatellite DNA
Autorzy:
Lesiczka, P.
Pawlaczyk, E.M.
Łabiszak, B.
Urbaniak, L.
Powiązania:
https://bibliotekanauki.pl/articles/1292493.pdf
Data publikacji:
2017
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
lesnictwo
Nadlesnictwo Milomlyn
drzewa lesne
sosna zwyczajna
Pinus sylvestris
zmiennosc
sosna taborska
igly sosny
cechy morfologiczne
DNA mikrosatelitarny
polimorfizm genetyczny
markery molekularne
genom jadrowy
genom chloroplastowy
Scots pine from Tabórz
needle morphology
nuclear (nrSSR) and chloroplast (cpSSR) microsatellite DNA
genetic
variation
Opis:
Scots pine (Pinus sylvestris L.) is one of the most widespread forest trees in the world, ranging from southern Mediterranean mountains to eastern Siberia. 30 trees of Scots pine from the Miłomłyn Forest District were analyzed in terms of 7 nuclear and 8 chloroplast microsatellite DNA loci as well as 7 morphological needle traits. Nuclear microsatellites (Simple Sequence Repeats) have proved to be useful in studying phylogeographic and gene flow patterns in conifers and are being used to infer the demographic history of tree species. A population’s genetic diversity, in fact, represents an important criterion which could be applied in planning future forest management and breeding. Additionally, many elements of Scots pine morphology, including needle traits, are subject to environmental modification. However, the adaptability of those traits remains, to some extent, under genetic control. The trees we investigated here are called Tabórz pine located in the northeast of Poland. This population is described as one of the best in Poland and even in Europe regarding bio mass production, ductility and wood quality. The aim of the study was to determine variation and genetic structure within this population. An analysis of phenotypic differentiation showed that the traits which differed the most between individuals were needle length, the number of serrations per 2 mm of needle length along the right edge and the number of stomatal rows on the convex side of the needles. In Tabórz pine, we detected 30 different genotypes with 87 alleles as well as 28 haplotypes with 30 alleles. We also revealed a slight excess of homozygotes, but the population is still in Hardy-Weinberg equilibrium. The Scots pine population from Tabórz furthermore showed a higher level of genetic diversity compared to stands from other Polish and European regions. This diversity may be the main factor impacting on the population’s wood quality and its breeding value.
Źródło:
Leśne Prace Badawcze; 2017, 78, 2
1732-9442
2082-8926
Pojawia się w:
Leśne Prace Badawcze
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zróżnicowanie fenotypowe i zmienność tła genetycznego polskich proweniencji sosny zwyczajnej
Phenotypic differentiation and genetic background variation of Polish provenances of Pinus sylvestris L.
Autorzy:
Hebda, A.
Skrzyszewski, J.
Wachowiak, W.
Powiązania:
https://bibliotekanauki.pl/articles/987201.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
hodowla lasu
drzewa lesne
sosna zwyczajna
Pinus sylvestris
proweniencje krajowe
zmiennosc fenotypowa
zmiennosc genetyczna
markery mikrosatelitarne
DNA mikrosatelitarny
polimorfizm loci
zmiennosc adaptacyjna
scots pine
phenotypic differentiation
neutral variation
microsatellite markers
environment correlation
local adaptation
Opis:
We looked at the patterns of differentiation at a set of phenotypic traits of Scots pine populations derived from different environmental conditions of the species distribution range in Poland. The growth traits were tested over 47 years at the provenance trial experiment in the Carpathians. The phenotypic differentiation of the populations was compared to diversity estimates at a set of ten nuclear microsatellite markers. Scots pine populations were highly differentiated concerning diameter at breast high, stand volume, stem straightness and crown width according to the prove− nance and climatic zones. At the same time a low genetic diversity for microsatellite markers and no evidence of population structure was found among proveniences. Local populations from the Carpathian Mountains had lower genetic variation and weaker growth compared to lowland provenances. Among the tested populations Scots pine from lowlands were characterized by the great growth potential and good adaptation to the severe climate of the Carpathians. Populations that showed high quantitative traits differentiation were genetically homogenous at the neutral loci. The results indicate that there are some regions in the genome under selection that drive species adaptive variation.
Źródło:
Sylwan; 2017, 161, 04; 277-286
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-11 z 11

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