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    Wyświetlanie 1-11 z 11
    Tytuł:
    Czy na plantacjach nasiennych zawężamy zmienność genetyczną? Próba odpowiedzi na podstawie analiz mikrosatelitarnego DNA szczepów rosnących na plantacji nasiennej sosny zwyczajnej (Pinus sylvestris L.) z Nadleśnictwa Susz
    Are we narrowing genetic variability in seed orchards? An attempt to answer, based on the analysis of microsatellite DNA of grafts growing in Scots pine (Pinus sylvestris L.) seed orchard in the Forest District Susz
    Autorzy:
    Przybylski, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/1311426.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    lesnictwo
    hodowla lasu
    plantacje nasienne
    sosna zwyczajna
    Pinus sylvestris
    zmiennosc genetyczna
    DNA mikrosatelitarny
    analiza DNA
    microsatellite DNA
    tree breeding
    seed orchard
    Opis:
    Scots pine (Pinus sylvestris L.) is the most common species in Poland’s forest stands. The mode of pine stands renovation requires that silviculture practitioners have continuous access to seed banks. Orchard-grown seeds are predicted to constitute an increasingly larger part of the average demand for pine seeds in Poland. Seed orchards, due to a limited number of maternal trees as well as the irregularity of their blooming and pollination, enhance the risk of genetic diversity reduction in planted forest stands. This is of particular importance in the context of dynamic climate change. Markers based on microsatellite DNA fragments are effective tools for monitoring genetic variability. In the present study, three different microsatellite DNA fragments were used: SPAC 12.5, SPAG 7.14 and SPAC 11.4. The main objective of this research was to study genetic variability in one of the biggest seed orchards in Poland, located in the Forest District Susz. The obtained results indicated heterozygosity loss within the orchard, proving the existence of specimen selection effects on genetic variability. Hence, it seems quite important to take account of molecular genetic variability of maternal trees in future breeding strategies.
    Źródło:
    Leśne Prace Badawcze; 2015, 76, 3; 240-249
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analysis of microsatellite instability and loss of heterozygosity in breast cancer with the use of a well characterized multiplex system.
    Autorzy:
    Powierska-Czarny, Jolanta
    Miścicka-Śliwka, Danuta
    Czarny, Jakub
    Grzybowski, Tomasz
    Woźniak, Marcin
    Drewa, Gerard
    Czechowicz, Włodzimierz
    Sir, Jan
    Powiązania:
    https://bibliotekanauki.pl/articles/1043412.pdf
    Data publikacji:
    2003
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    microsatellite instability
    short tandem repeats
    multiplex DNA typing
    breast cancer
    Opis:
    Analysis of microsatellite instability (MI) and loss of heterozygosity (LOH) is recommended for screening patients with sporadic and hereditary malignancies. This study shows an application of a fluorescent hexaplex PCR system for microsatellite typing on A.L.F. DNA Sequencer (Pharmacia Biotech). This technique detects changes in microsatellites providing a time-efficient, reliable and accurate method for MI and LOH analyses. The Fragment Manager software was used for automated size calculation and quantitation of DNA fragments, enabling rapid and precise measurement of allelic ratios. We examined 70 breast cancer and 70 control DNA specimens, classified all the patterns of microsatellite alterations, and set up MI and LOH assessment criteria for the automated multiplex fluorescent method.
    Źródło:
    Acta Biochimica Polonica; 2003, 50, 4; 1195-1203
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mitochondrial and nuclear DNA differentiation of Picea abies populations in Poland
    Autorzy:
    Nowakowska, J A
    Powiązania:
    https://bibliotekanauki.pl/articles/40976.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    international conference
    Europe
    forest ecosystem
    plant breeding
    tree
    Norway spruce
    Picea abies
    Polska
    mitochondrial DNA
    nuclear DNA
    plant population
    microsatellite marker
    post-glacial migration
    Opis:
    The natural stands of Norway spruce in Poland are split between the southern and the northeastern parts of the country. Two so-called "spruceless" zones separate the northern spruce locations from those in the south, one "spruceless" zone is situated in Central Poland, and the other one in the Beskid Mts. Mitochondrial (STS) and nuclear (SSR) markers were used to perform the genetic identification of Norway spruce. Four different variants of haplotypes, "a", "b", "c" and "d", were found to occur in the nad1 locus of STS markers. Populations from the northern range of Picea abies distribution in Poland harboured exclusively haplotypes "c" and "d", except for the Białowieża population which had haplotypes "a" and "c". Populations from the "spruceless" zones contained four types of haplotypes whilst those from southern Poland were mostly composed of haplotype "a". High mean gene diversity was observed for both STS and SSR markers (HT = 0.529, and HT = 0.851, respectively). The total genetic differentiation of Norway spruce populations was very low (FST= 0.088). Two main groups of populations were distinguished in the dendrogram defined by Nei's genetic distances based on microsatellite markers. The distribution of the genotypes was scattered and did not show any connection with the spatial distribution of P. abies in Poland. Only the mtDNA markers were able to differentiate the northern populations of Norway spruce from the southern ones, proving the historical separation between the Baltico-Nordic and the Hercyno-Carpathian ranges of P. abies in Poland. By contrast, the microsatellite data suggested an overlap between the genotypes due to the human manipulation of Norway spruce stands in the past.
    Źródło:
    Dendrobiology; 2009, 61 Supplement
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zmienność taborskiej sosny zwyczajnej (Pinus sylvestris L.) z Nadleśnictwa Miłomłyn wyrażona w analizie cech morfologii igieł oraz polimorfizmie mikrosatelitarnego DNA
    Variability of Scots pine (Pinus sylvestris L.) called Taborz pine (Forest District Milomlyn) expressed in analysis of morphology of needle traits and polymorphism of microsatellite DNA
    Autorzy:
    Lesiczka, P.
    Pawlaczyk, E.M.
    Łabiszak, B.
    Urbaniak, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/1292493.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    lesnictwo
    Nadlesnictwo Milomlyn
    drzewa lesne
    sosna zwyczajna
    Pinus sylvestris
    zmiennosc
    sosna taborska
    igly sosny
    cechy morfologiczne
    DNA mikrosatelitarny
    polimorfizm genetyczny
    markery molekularne
    genom jadrowy
    genom chloroplastowy
    Scots pine from Tabórz
    needle morphology
    nuclear (nrSSR) and chloroplast (cpSSR) microsatellite DNA
    genetic
    variation
    Opis:
    Scots pine (Pinus sylvestris L.) is one of the most widespread forest trees in the world, ranging from southern Mediterranean mountains to eastern Siberia. 30 trees of Scots pine from the Miłomłyn Forest District were analyzed in terms of 7 nuclear and 8 chloroplast microsatellite DNA loci as well as 7 morphological needle traits. Nuclear microsatellites (Simple Sequence Repeats) have proved to be useful in studying phylogeographic and gene flow patterns in conifers and are being used to infer the demographic history of tree species. A population’s genetic diversity, in fact, represents an important criterion which could be applied in planning future forest management and breeding. Additionally, many elements of Scots pine morphology, including needle traits, are subject to environmental modification. However, the adaptability of those traits remains, to some extent, under genetic control. The trees we investigated here are called Tabórz pine located in the northeast of Poland. This population is described as one of the best in Poland and even in Europe regarding bio mass production, ductility and wood quality. The aim of the study was to determine variation and genetic structure within this population. An analysis of phenotypic differentiation showed that the traits which differed the most between individuals were needle length, the number of serrations per 2 mm of needle length along the right edge and the number of stomatal rows on the convex side of the needles. In Tabórz pine, we detected 30 different genotypes with 87 alleles as well as 28 haplotypes with 30 alleles. We also revealed a slight excess of homozygotes, but the population is still in Hardy-Weinberg equilibrium. The Scots pine population from Tabórz furthermore showed a higher level of genetic diversity compared to stands from other Polish and European regions. This diversity may be the main factor impacting on the population’s wood quality and its breeding value.
    Źródło:
    Leśne Prace Badawcze; 2017, 78, 2
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tracing the genetic origin of brown trout (Salmo trutta) re-colonizing the Ecker reservoir in the Hartz National Park, Germany
    Autorzy:
    Kohlmann, K.
    Wüstemann, O.
    Powiązania:
    https://bibliotekanauki.pl/articles/363212.pdf
    Data publikacji:
    2012
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    pstrąg potokowy
    Salmo trutta
    mikrosatelitarne DNA
    DNA
    PCR
    brown trout
    microsatellite
    Opis:
    The Ecker reservoir and its main tributary had been free of brown trout (Salmo trutta) for several decades due to cumulative effects of natural and anthropogenic acidification. However, after the decline of emissions in the 1990s and the resulting rise of water pH to suitable for brown trout values, the species began to recolonize its original habitats. In the main tributary first brown trout individuals were caught in 2008 and in the reservoir in later years as well. Stocking could be excluded in both areas. Therefore, the present study was aimed to trace the genetic origin of these brown trout by genotyping eight microsatellite loci in samples collected in the reservoir, its main tributary, potential refugia and - for comparison - from two areas downstream of the dam being physically isolated for about 70 years. Genetic variability within populations (average number of alleles per locus), genetic differentiation between populations (FST values and genetic distances), occurrence of certain alleles and results of assignment tests indicated that the Ecker reservoir was re-colonized from two sources: the Große Peseke, a small direct inflow into the reservoir, and the Fuhler Lohnsbach, a parallel flowing brook connected to the reservoir by a pipe. Genetic data also supported recolonization of the main tributary from the reservoir but not in the opposite direction. Moreover, bottleneck effects were evident in brown trout populations upstream of the dam compared to the two populations downstream of the dam.
    Źródło:
    Environmental Biotechnology; 2012, 8, 2; 39-44
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    New multiplex PCR assays for estimating genetic diversity in rainbow trout (Oncorhynchus mykiss) by polymorphism of microsatellite DNA
    Autorzy:
    Kaczmarczyk, D.
    Kaczor, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/363262.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    microsatellite DNA
    multiplex PCR
    population genetics
    rainbow trout
    DNA mikrosatelitarne
    genetyka populacyjna
    pstrąg tęczowy
    Opis:
    Multiplex PCR is a useful technique for estimating genetic diversity. This paper presents 3 new sets of primer pairs for effectively amplifying 10 microsatellite DNA loci from rainbow trout (Oncorhynchus mykiss). Unlike other sets of primer pairs that have been developed for amplifying rainbow trout microsatellite loci, ours do not require the hot-start PCR technique. In the paper, we describe the steps taken to choose the loci for each multiplex assay and to verify the genotyping results. We provide the compositions of the PCR mixture and the characteristics of the PCR thermal profile recommended for amplification.
    Źródło:
    Environmental Biotechnology; 2013, 9, 1; 19-24
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Polymorphism of microsatellite loci - a tool in studying biodiversity of paddlefish aquaculture broodstock
    Autorzy:
    Kaczmarczyk, D.
    Kohlmann, K.
    Kersten, P.
    Luczynski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363088.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    izolacja DNA
    DNA mikrosatelitarne
    amplifikacja PCR
    DNA extraction
    microsatellite DNA
    PCR amplification
    Opis:
    American paddlefish (Polyodon spathula) is a new species in Polish aquaculture, its broodstocks are few and small, and it is possible that all mature fish originated from only a few spawners. Studies on polymorphism of highly variable microsatellite DNA allow revealing genetic characteristics of individual spawners as well as estimation of genetic variation within and divergence between broodstocks. This paper describes optimised protocols for isolation of DNA from fin tissues, amplification of nine microsatellite loci using PCR technique, and for fish genotyping using automatic capillary DNA sequencer. Our technique was tested towards the fin samples taken from all paddlefish reared in Poland and approaching their sexual maturity; the study included also samples taken from 47 fish of the Ukrainian breeding center (Gorny Tykich).
    Źródło:
    Environmental Biotechnology; 2007, 3, 2; 44-48
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Monitoring of cellular chimerism in patients after sex-mismatched bone marrow transplantation: technical report
    Autorzy:
    Jolkowska, J
    Ladon, D.
    Wachowiak, J.
    Witt, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2043435.pdf
    Data publikacji:
    2000
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    in situ
    allogeneic transplantation
    chimerism
    molecular analysis
    transplantation
    minimal residual disease
    acute myeloblastic leukemia
    polymorphic microsatellite
    bone marrow
    hybridization
    polymerase chain reaction
    DNA
    detection
    Źródło:
    Journal of Applied Genetics; 2000, 41, 3; 209-212
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zróżnicowanie fenotypowe i zmienność tła genetycznego polskich proweniencji sosny zwyczajnej
    Phenotypic differentiation and genetic background variation of Polish provenances of Pinus sylvestris L.
    Autorzy:
    Hebda, A.
    Skrzyszewski, J.
    Wachowiak, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/987201.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    hodowla lasu
    drzewa lesne
    sosna zwyczajna
    Pinus sylvestris
    proweniencje krajowe
    zmiennosc fenotypowa
    zmiennosc genetyczna
    markery mikrosatelitarne
    DNA mikrosatelitarny
    polimorfizm loci
    zmiennosc adaptacyjna
    scots pine
    phenotypic differentiation
    neutral variation
    microsatellite markers
    environment correlation
    local adaptation
    Opis:
    We looked at the patterns of differentiation at a set of phenotypic traits of Scots pine populations derived from different environmental conditions of the species distribution range in Poland. The growth traits were tested over 47 years at the provenance trial experiment in the Carpathians. The phenotypic differentiation of the populations was compared to diversity estimates at a set of ten nuclear microsatellite markers. Scots pine populations were highly differentiated concerning diameter at breast high, stand volume, stem straightness and crown width according to the prove− nance and climatic zones. At the same time a low genetic diversity for microsatellite markers and no evidence of population structure was found among proveniences. Local populations from the Carpathian Mountains had lower genetic variation and weaker growth compared to lowland provenances. Among the tested populations Scots pine from lowlands were characterized by the great growth potential and good adaptation to the severe climate of the Carpathians. Populations that showed high quantitative traits differentiation were genetically homogenous at the neutral loci. The results indicate that there are some regions in the genome under selection that drive species adaptive variation.
    Źródło:
    Sylwan; 2017, 161, 04; 277-286
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic identification of black caviar based on microsatellite DNA analysis
    Autorzy:
    Fopp-Bayat, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/363094.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    identyfikacja genetyczna
    czarny kawior
    DNA mikrosatelitarne
    genetic identification
    black caviar
    microsatellite DNA
    Opis:
    Sturgeons (Acipenseridae) are producers of black caviar, which is sold world-wide. Black caviar differs widely in quality, availability, price and taste and for these reasons it is often the subject of commercial fraud. Identification of sturgeon species is frequently based on molecular methods such as analysis of nuclear DNA and mitochondrial DNA. In this study two fragments of microsatellite DNA: Afu-39 and Afu-68 were analyzed. In caviar samples, four alleles were observed in locus Afu-39, and six alleles in locus Afu-68. This paper describes the use of microsatellite DNA markers for species identification of black caviar.
    Źródło:
    Environmental Biotechnology; 2007, 3, 2; 57-60
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microsatellite DNA polymorphism in sturgeon species and their hybrids reared in Polish aquaculture farms
    Autorzy:
    Fopp-Bayat, D.
    Luczynski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363194.pdf
    Data publikacji:
    2006
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    jesiotr
    identyfikacja gatunkowa
    DNA mikrosatelitarne
    hybrydy
    polimorfizm
    hybrids
    microsatellite DNA
    polymorphism
    PCR
    species identification
    sturgeons
    Opis:
    Highly variable microsatellite DNA loci show high levels of variation per locus and provide molecular markers for species and for populations of small effective size. In this study we applied microsatellite markers to identify specimens belonging to several sturgeon species and their interspecific hybrids. Nine microsatellite loci (Afu-19, Afu-22, Afu-34, Afu-39, Afu-54, Afu-57, Afu-58, Afu-68, Afu-69) were analysed for five species (beluga, Siberian sturgeon, Russian sturgeon, sterlet and paddlefish) and for their three interspecific hybrids: Siberian sturgeon x Russian sturgeon, beluga x sterlet (called bester) and for beluga x bester. Certain alleles at five loci: Afu-22, Afu-39, Afu-54, Afu-57 and Afu-68 were diagnostic for the examined sturgeon species and their interspecific hybrids.
    Źródło:
    Environmental Biotechnology; 2006, 2, 1; 11-19
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-11 z 11

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