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    Tytuł:
    Stability of cyanotoxins, microcystin-LR, microcystin-RR and nodularin in seawater and BG-11 medium of different salinity
    Autorzy:
    Mazur, H.
    Plinski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/48388.pdf
    Data publikacji:
    2001
    Wydawca:
    Polska Akademia Nauk. Instytut Oceanologii PAN
    Tematy:
    cyanotoxin
    microcystin-LR
    biodegradation
    microcystin-RR
    Gdansk Gulf
    Cyanoprokaryota
    hepatotoxin
    sea water
    nodularin
    Opis:
    Microcystins and nodularin are potent hepatotoxins produced by fresh and seawater cyanobacteria.T he persistence of three hepatotoxins – microcystin-LR, microcystin-RR and nodularin – was investigated in sterile BG-11 medium of different salinity and in water collected from the Gulf of Gdańsk.After 21 days of incubation at 17±1◦ C and constant illumination of about 40 μmol photon m−2 s−1 the concentration of toxins decreased by about 30–37%.N o significant changes in toxin concentration in the BG-11 media of different salinity were observed. When toxins were incubated in non-sterile seawater, their concentrations decreased markedly.It is likely that some strains of bacteria are responsible for the breakdown of the toxins.No dularin turned out to be more resistant to biodegradation than the two microcystins.The influence of certain components of cyanobacteria cells on the accelerated rate of toxin degradation was also considered.
    Źródło:
    Oceanologia; 2001, 43, 3; 329-339
    0078-3234
    Pojawia się w:
    Oceanologia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary Investigation of Bioaccumulation of Microcystins in Hypereutrophic Irrigation Ponds Case Study – the Jordan Valley
    Autorzy:
    Al Kuisi, Mustafa
    Hyarat, Tasneem
    Al Qaisi, Khaled
    Alhejoj, Ikhlas
    Al-Rshaidat, Mamoon
    Powiązania:
    https://bibliotekanauki.pl/articles/24201662.pdf
    Data publikacji:
    2023
    Wydawca:
    Polskie Towarzystwo Inżynierii Ekologicznej
    Tematy:
    Jordan Valley
    eutrophication
    cyanobacteria
    microcystin-LR
    irrigation pond
    wastewater
    Opis:
    Microcystis blooms and the related toxin known as microcystin-LR (MC-LR) put the safety of human water consumption and global irrigation practices in jeopardy. MC-LR is widely distributed in various environments, including water, sediments, plants, and other aquatic organisms. The use of water-containing microcystins for agricultural purposes may have to be restricted despite the limited availability of clean water resources. Accordingly, the present work aimed to determine the MC-LR concentrations and recognize the environmental parameters that initiate the growth of toxic cyanobacteria and MC-LR occurrence in 20 irrigation ponds in the Jordan Valley area. The irrigation ponds studied were found in a hypereutrophic condition, with high levels of N:P ratio and low transparency. These cause inseparable effects such as cyanobacterial bloom and MC-LR occurrence. The investigated ponds were classified as hypereutrophic according to General Quality Index (GQI), with two different types of algae covering the surface. The first was the Lemna sp. or duckweeds (Family Araceae) which are free-floating masses, and the second was the cyanobacteria algal bloom. Unpaired t-tests were performed and showed that the concentrations of MC-LR in pond water abundant with cyanobacteria algal bloom in September 2021 were significantly higher (P = 0.7906) than in June for the same year (0.3022 ± 0.0444 and 0.1048 ± 0.0171 ppb, respectively). Two methods for extracting MC-LR were used and showed a significant difference in MC-LR concentration in ponds with an abundance of cyanobacteria algal blooms (0.2273 ± 0.0356 ppb) compared to the ponds with an abundance of Lemna sp. or duckweeds collected in June 2021 (0.1048 ± 0.0171 ppb). Despite all of the efforts made by Jordan Valley farmers to prevent or limit the mass growth of cyanobacteria and its consequences for the eutrophication process in their irrigation ponds through the use of fish breading and chemicals such as copper sulfate, this environmental problem is still harming their crops and irrigation methods and requires immediate government assistance.
    Źródło:
    Journal of Ecological Engineering; 2023, 24, 4; 36--53
    2299-8993
    Pojawia się w:
    Journal of Ecological Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Functional investigation of MiR92b-3p for diagnosis and miRNA-based cure in chemically induced liver injury in fish: a project description
    Autorzy:
    Brzuzan, P.
    Woźny, M.
    Florczyk, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363176.pdf
    Data publikacji:
    2016
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    hepatotoxicity mechanism
    liver regeneration
    Locked Nucleic Acids
    microcystin
    RNA interference
    toxicogenomics
    whitefish
    Opis:
    The continued lack of knowledge concerning the molecular background of adverse effects caused by microcystin-LR (MC-LR) is surprising. This toxin requires additional attention, not only for its ability to cause acute poisoning, but also for its ability to initiate cancer in acute doses, and potentially, to promote cancer via chronic exposure to low concentrations in drinking water. Our recent studies on whitefish (Coregonus lavaretus) revealed that long-term exposure to MC-LR resulted in severe liver injury, followed by regeneration of the liver and its unexpected resilience to further toxin uptake. These effects were accompanied by perturbations of hepatic microRNAs (miRNAs) that have target genes involved in cytoskeletal remodeling, cell metabolism, cell cycle regulation, and apoptosis. Among the most pronounced individual alterations, the reduction of MiR92b-3p expression was the most remarkable, and we suggest roles for the miRNA in the aberrant processes of liver cells. This project addresses potential involvement of MiR92b-3p in the as yet unknown regulatory network of MC-induced hepatotoxicity in fish. After a suite of biochemical, physiological, anatomical, and transcriptomic analyses in vitro and in vivo, we will show how MiR92b-3p works in a damaged liver and which processes it targets. Finally, the research will confirm if and how MiR92b-3p can be targeted therapeutically. We expect it to be shown effective enough to pave a way for its use as a tool for treatment of liver damage in fish. What is more, the RNA-based silencing technique that will be used should yield exciting data for our understanding of the system-level biology of vertebrates.
    Źródło:
    Environmental Biotechnology; 2016, 12, 2; 40-42
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Neurotoxicity of cyanobacterial toxins
    Autorzy:
    Florczyk, M.
    Łakomiak, A.
    Woźny, M.
    Brzuzan, P.
    Powiązania:
    https://bibliotekanauki.pl/articles/363222.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    blue-green algae
    cyanotoxins
    drug
    medicine
    microcystin
    neurotoxicity
    sinice
    cyjanotoksyny
    lek
    mikrocystyna
    neurotoksyczność
    Opis:
    Eutrophication of marine and fresh waters can lead to excessive development of cyanobacterial blooms, which may contain strains that produce toxins. These toxins are secondary metabolites which can accumulate in the food chain and contaminate drinking water, thus posing a potential threat to the health of humans and aquatic organisms. These toxins include a variety of compounds with different mechanisms; this review focuses on the neurotoxicity of microcystin and other cyanotoxins. Although the hepatotoxic action of microcystins is commonly known, its neurotoxic effects have also been described, e.g. oxidative stress, cytoskeletal changes and changes in protein phosphatase activity. These effects have been partially explained by the discovery in the blood brain barrier of the same membrane transporters involved in microcystins hepatotoxic mechanisms. Additionally, this paper reviews other cyanotoxins that are known or suspected to target cholinergic synapses and voltage gated channels, including anatoxin a, anatoxin a(s), antillatoxins, cylindrospermopsin, homoanatoxin a, jamaicamide, kalkitoxin and saxitoxins. The neurotoxic and cytotoxic effects of the cyanotoxins discussed here are of particular interest because of their pharmacological potential. This review also discusses the potential of these compounds to serve as drugs for cancer and central nervous system failure.
    Źródło:
    Environmental Biotechnology; 2014, 10, 1; 26-43
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Badania nad skutecznością usuwania mikrocystyny LR w układzie oczyszczania wody w Tomaszowie Mazowieckim
    Efficiency of Microcystin LR Removal from Surface Water Treated by the Tomaszów Mazowiecki Water Treatment Plant
    Autorzy:
    Kabziński, A.
    Macioszek, B.
    Szczukocki, D.
    Juszczak, R.
    Skowron, W.
    Zawadzka, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/237526.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Zrzeszenie Inżynierów i Techników Sanitarnych
    Tematy:
    wody powierzchniowe
    sinice
    mikrocystyna LR
    oczyszczanie wody
    ozonowanie
    surface water
    cyanobacteria
    microcystin LR
    water treatment
    ozonation
    Opis:
    W normalnym stanie wód powierzchniowych liczebność komórek sinic (Cyanophyta) w 1 cm3 wody waha się od kilkuset do kilku tysięcy, przy czym w okresach intensywnego zakwitu może dochodzić do kilkuset tysięcy na 1 cm3 wody. Intensywne zakwity mogą powodować zmianę barwy i nieprzyjemny zapach wody, a także uwalniają duże ilości neurotoksyn alkaloidowych i hepatotoksyn o wysokiej toksyczności. W pracy przedstawiono wyniki badań dotyczące skuteczności oczyszczania wody powierzchniowej w obecności komórek sinic i mikrocystyny LR w układzie oczyszczania wody w Tomaszowie Mazowieckim w 2004 r. Wykazano, że zastosowanie koagulacji bez wstępnej dezynfekcji chlorem lub dwutlenkiem chloru powodowało wzrost zawartości mikrocystyny LR w wodzie (śr. o 176%), w wyniku rozpadu (lizy) komórek sinic pod wpływem koagulantu. Ozonowanie wody dawką 3,0÷5,5 gO3/m3 było najefektywniejszym etapem usuwania mikrocystyny LR z wody. Końcowa efektywność usuwania mikrocystyny LR z wody zmieniała się w zakresie 49,8÷100,0%.
    Normally, the number of cyanobacterial cells (Cyanophyta) in surface water varies from several hundred to several thousand per cubic centimeter. While blooming, their number may rise to several hundred thousand per cubic centimeter of water, and the water may take a specific hue and an unpleasant smell. In addition, during blooming cyanobacteria produce large amounts of alkaloid neurotoxins and oligopeptide hepatotoxins of high toxicity. The study reported on in this paper was carried out at the Water Treatment Plant of Tomaszów Mazowiecki in 2004. It aimed at determining the efficiency of their treatment train when the surface water to be treated contained cyanobacterial cells and microcystin LR. The investigations have produced the following findings: coagulation without prechlorination (with chlorine or chlorine dioxide) promoted a rise in microcystin LR concentration by 176% on average, as a result of cell lysis due to exposure to the coagulant; ozonation (with a 3.0 to 5.5 gO3/m3) was the most efficient unit process for microcystin LR removal. The final efficiency of microcystin LR removal from the water ranged from 49.8 to 100%.
    Źródło:
    Ochrona Środowiska; 2005, R. 27, nr 3, 3; 39-42
    1230-6169
    Pojawia się w:
    Ochrona Środowiska
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Toxic cyanobacteria blooms in the Lithuanian part of the Curonian Lagoon
    Autorzy:
    Paldaviciene, A.
    Mazur-Marzec, H.
    Razinkovas, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/48516.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Oceanologii PAN
    Tematy:
    Baltic Sea
    blue-green alga
    Curonian Lagoon
    Microcystis aeruginosa
    Nodularia spumigena
    Cyanoprokaryota
    bloom
    microcystin
    Lithuania
    nodularin
    Opis:
    The phenomenon of cyanobacteria (blue-green algae) blooms in the Baltic and the surrounding freshwater bodies has been known for several decades.The presence of cyanobacterial toxic metabolites in the Curonian Lagoon has been investigated and demonstrated for the first time in this work (2006–07). Microcystis aeruginosa was the most common and widely distributed species in the 2006 blooms. Nodularia spumigena was present in the northern part of the Curonian Lagoon, following the intrusion of brackish water from the Baltic Sea; this is the first time that this nodularin-(NOD)-producing cyanobacterium has been recorded in the lagoon.W ith the aid of high-performance liquid chromatography (HPLC), four microcystins (MC-LR, MC-RR, MC-LY, MC-YR) and nodularin were detected in 2006.T he presence of these cyanobacterial hepatotoxic cyclic peptides was additionally confirmed by enzyme-linked immunosorbent assay (ELISA) and protein phosphatase inhibition assay (PP1).Micr ocystin-LR, the most frequent of them, was present in every sample at quite high concentrations (from <0.1 to 134.2 μg dm−3).I n 2007, no cyanobacterial bloom was recorded and cyanotoxins were detected in only 4% of the investigated samples.A comparably high concentration of nodularin was detected in the northern part of the Curonian Lagoon.I n one sample dimethylated MC-RR was also detected (concentration 7.5 μg dm−3).
    Źródło:
    Oceanologia; 2009, 51, 2; 203-216
    0078-3234
    Pojawia się w:
    Oceanologia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    MicroRNA expression in liver of whitefish (Coregonus lavaretus) exposed to microcystin-LR
    Autorzy:
    Brzuzan, P.
    Woźny, M.
    Wolińska, L.
    Piasecka, A.
    Łuczyński, M. K.
    Powiązania:
    https://bibliotekanauki.pl/articles/363280.pdf
    Data publikacji:
    2010
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    Coregonus lavaretus
    Dicer
    histon H2A
    mikrocystyna LR
    miRNAs
    toksyczność
    histone H2A
    microcystin LR
    toxicity
    Opis:
    MicroRNAs (miRNAs) are small, highly conserved, non-coding RNAs that regulate gene expression of target mRNAs through cleavage or translational inhibition. In the field of toxicology, the relationship between toxicity and microRNA expression is poorly understood. In the present study we analyzed the abundance of 9 selected miRNAs (omy-miR-21, omy-miR-21t, omy-miR-122, omy-miR-125a, omy-miR-125b, omy-miR-125t, omy-miR-199-5a, omy-miR-295, omy-let-7a) and mRNA of 3 genes (histone H2A, ribosome protein rpl19, and Dicer which is a miRNA processing enzyme) in liver samples of whitefish exposed to Microcystin-LR (MC-LR) at a dose of 100µg*kg-1body weight for 24 or 48h. In the examined liver tissue, omymiR-122 showed the highest relative constitutive level, what is consistent with data obtained from fish and mammals. Unexpectedly, the reference H2A mRNA level was consistently up-regulated (over 20-fold; P<0.05) in fish liver after both 24 and 48h of exposure to MC-LR. The result may suggest that MC-LR acts as an initiator of specific cell-physiologic signals triggering DNA replication in fish liver cells. MC-LR treatment had no effect on the examined miRNAs levels, except for omy-miR-125a and omy-let-7a. Whereas omy-miR-125a was up-regulated (ER=2.68; S.E. 1.61-6.78; P<0.05), omy-let-7a was down-regulated (ER=0.55; S.E. 0.32-0.79; P<0.05) in whitefish liver after 48h of the treatment with MC-LR, when compared to controls. More work with the fish is essential for understanding the crosstalk of the regulatory network controlled by the two miRNAs in the context of MC-LR toxicity.
    Źródło:
    Environmental Biotechnology; 2010, 6, 2; 53-60
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Morphological, genetic, chemical and ecophysiological characterisation of two Microcystis aeruginosa isolates from the Vistula Lagoon, Southern Baltic
    Autorzy:
    Mazur-Marzec, H.
    Browarczyk-Matusiak, G.
    Forycka, K.
    Kobos, J.
    Plinski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/48489.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Instytut Oceanologii PAN
    Tematy:
    Microcystis aeruginosa
    Cyanoprokaryota
    microcystin
    mcy gene
    morphology
    Vistula Lagoon
    Baltic Sea
    eutrophic water
    bloom
    16S rRNA sequence
    internal transcribed spacer
    Opis:
    The Vistula Lagoon (southern Baltic Sea) is a shallow and highly eutrophic water body, with frequent blooms of cyanobacteria dominated by Microcystis and Anabaena species. Two Microcystis strains, MK10.10 and MAKR0205, isolated from the lagoon were characterised in this work. The morphology of the isolates differed significantly with respect to cell size and their ability to form aggregates. Based on the 16S rRNA sequence and 16S-23S internal transcribed spacer (ITS) sequence, both isolates were classified as Microcystis aeruginosa. However, only one isolate, MK10.10, possessed the mcy genes responsible for microcystin biosynthesis and only this strain produced microcystins. The effects of environmental factors, such as light, temperature and salinity, on toxin production turned out to be minor. Under the culture conditions used in the experiments, the biomass of the toxic MK10.10 was always lower. Hybrid quadrupole-time-of-flight liquid chromatography/tandem mass spectrometry (QTOF-LC/MS/MS) was used to elucidate the structure of the microcystin (MC) variants produced by MK10.10. Based on molecular ion and fragmentation spectra, the toxins were identified as MC-LR, MC-VR and MC- HIlR. Our study confirmed that some morphological criteria could be useful in preliminarily assessing the potential toxicity of a Microcystis bloom.
    Źródło:
    Oceanologia; 2010, 52, 1; 127-146
    0078-3234
    Pojawia się w:
    Oceanologia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bioaccumulation of microcystins in invasive bivalves: A case study from the boreal lagoon ecosystem
    Autorzy:
    Paldaviciene, A.
    Zaiko, A.
    Mazur-Marzec, H.
    Razinkovas-Baziukas, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/47622.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Instytut Oceanologii PAN
    Tematy:
    microcystin
    mussel tissue
    zebra mussel
    bioaccumulation
    bivalve
    Dreissena polymorpha
    brackish water
    eutrophic water
    Curonian Lagoon
    Baltic Sea
    environment condition
    biomonitoring
    protein phosphatase
    ELISA test
    Źródło:
    Oceanologia; 2015, 57, 1
    0078-3234
    Pojawia się w:
    Oceanologia
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Application of Biotests in Cyanobacterial Extract Toxicity Assessment
    Zastosowanie biotestów do oceny toksyczności ekstraktu uzyskanego z komórek sinic
    Autorzy:
    Sierosławska, A.
    Rymuszka, A.
    Skowroński, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/204758.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    cyanobacterial bloom
    cyanotoxins
    microcystin LR
    anatoxin-a
    biotests
    Daphnia magna
    Thamnocephalus platyurus
    Brachionus calycifl orus
    Tetrahymena thermophila
    cyjanobakteria
    zakwit sinic
    biotest
    mikrocystyna LR
    Daphtoxkit F magna
    Thamnotoxkit F
    Rotoxkit F
    Protoxkit F
    Opis:
    The aim of the study was to determine the toxicity of the extract obtained from the cyanobacterial cells derived from the waters of Zemborzycki dam reservoir with use of a battery of biotests. The taxonomic identification of the bloom-forming cyanobacteria revealed high abundance of Aphanizomenon flos-aquae and Dolichospermum spp. (Anabaena spp.) and in a lower degree of Microcystis aeruginosa and Planktothrix agardhii. In the extract obtained from concentrated cyanobacterial cells, hepatotoxin microcystin-LR at a concentration of 22.89 ± 3.74 μg/L and neurotoxin Antx-a at 13.02 ± 0.01 μg/L have been detected. Toxicity of the extract was evaluated with the following assays: Daphtoxkit F magna with the crustacean Daphnia magna, Thamnotoxkit F with the crustacean Thamnocephalus platyurus, Rotoxkit F with the rotifer Brachionus calyciflorus and Protoxkit F with ciliate Tetrahymena thermophila. The most sensitive organism among all studied was T. platyurus for which EC50 was estimated to be 1.2% of the initial extract concentration. On the basis of the highest obtained value of the toxicity unit (TU = 83) the studied sample was classified to the IV class, which is of high acute toxicity. Additionally, it was found that reactivity on cyanobacterial products differs greatly among organisms used in bioassays, which indicate the need for using a set of biotests.
    Celem pracy była ocena toksyczności ekstraktu uzyskanego z komórek cyjanobakterii obecnych w wodzie pobranej podczas zakwitu sinic z Zalewu Zemborzyckiego, z wykorzystaniem zestawu biotestów. Identyfikacja taksonomiczna wykazała w badanej próbce wysoką liczebność Aphanizomenon fl os-aquae i Dolichospermum spp. (Anabaena spp.) oraz w mniejszym stopniu Microcystis aeruginosa i Planktothrix agardhii. Analiza HPLC ekstraktu uzyskanego z zagęszczonych komórek cyjanobakterii wykazała obecność mikrocystyny-LR w stężeniu 22,89 μg/L oraz anatoksyny-a w stężeniu 13.02 μg/L. Stopień toksyczności uzyskanego ekstraktu oceniano na podstawie reakcji organizmów testowych, z użyciem następujących biotestów: Daphtoxkit F magna (Daphnia magna), Thamnotoxkit F (Thamnocephalus platyurus), Rotoxkit F (Brachionus calyciflorus) oraz Protoxkit F (Tetrahymena thermophila) (Microbiotests Inc., Belgia). Przeprowadzone badania wykazały szczególnie silne działanie toksyczne analizowanego ekstraktu wobec T. platyurus (24hEC50 oszacowane na 1,2% ekstraktu wyjściowego). Wyraźny efekt toksyczny obserwowany był także wobec B. calyciflorus i w mniejszym stopniu wobec T. thermophila. Natomiast największą odporność na toksyczne działanie ekstraktu wykazywała D. magna. Na podstawie uzyskanych wartości TU (jednostek toksyczności) badany ekstrakt zaliczony został do IV klasy toksyczności, tj. o wysokiej toksyczności ostrej. Z przeprowadzonych badań wynika, że organizmy na których wykonywane są testy toksyczności wykazują zróżnicowaną wrażliwość na produkty cyjanobakterii, stąd ocena toksyczności próbek zawierających tego typu substancje powinna być prowadzona z wykorzystaniem jak najszerszego zestawu biotestów.
    Źródło:
    Archives of Environmental Protection; 2014, 40, 3; 115-121
    2083-4772
    2083-4810
    Pojawia się w:
    Archives of Environmental Protection
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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