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    Wyświetlanie 1-7 z 7
    Tytuł:
    Field and Laboratory Studies of Encysted and Trophic Stages of Naked Amoebae: Including a Perspective on Population Life Cycle Dynamics
    Autorzy:
    Anderson, O. Roger
    Powiązania:
    https://bibliotekanauki.pl/articles/763583.pdf
    Data publikacji:
    2010
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Eukaryotic microbial communities, microbial ecology, microbial survival strategies, protistan community succession
    Opis:
    Naked amoebae are among the most abundant soil protists, especially in highly productive soils. Their capacity to produce cysts during unfavorable growth periods, especially drying, enhances their survivability as resting stages and promotes dispersal by wind and air currents. However, the dynamics of their cycles of encystment and active growth are poorly documented. Using a recently developed culture observation method, including a dried preparation stage to detect encysted amoebae, data are presented on the ratios of active and encysted stages of naked amoebae based on field samples from diverse terrestrial sites differing in plant cover and moisture content during spring and summer months 2008 at a location in northeastern U.S.A. Percentage of encysted amoebae varied between 32% and 100% depending on locale and moisture content. Carbon content of the cysts (estimated from recently excysted individuals) relative to trophic stages varied between 22% and 100% at these same locales. Laboratory experimental studies of winter soil samples, that were cultured at 25oC to promote amoeba community growth, indicated that a dynamic relationship exists between active and encysted stages during proliferation with varying ratios depending on the moisture content and qualities of the soil at the collection site, thus suggesting a revised model as presented here of the encystment-excystment cycle for populations during a growth succession.
    Źródło:
    Acta Protozoologica; 2010, 49, 1
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Identifying microbes from environmental water samples
    Autorzy:
    Mungs, Wenfa
    Powiązania:
    https://bibliotekanauki.pl/articles/1153739.pdf
    Data publikacji:
    2015
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    biomarker
    educational research
    inquiry-based
    mass spectrum fingerprinting
    microbial ecology
    microbial typing
    phylogeny
    ribosomal proteins
    scientific method
    taxonomy
    Opis:
    What is the microbe that we are dealing with? Whether it is cholera or anthrax, we want to know the disease-causing microorganism as quickly as possible since prompt identification of the causative organism would help control disease spread - and potentially save lives through provision of appropriate care and medication. Yet, despite the advent of rapid microbial identification tools – particularly those based on mass spectrometry – most undergraduate curricula continue to focus on culture- and nucleic acid-based identification techniques since they are widely used for detecting and identifying microbes in clinical and environmental samples. Mass spectrometry-based methods, however, have increasingly complemented traditional approaches in clinical and research laboratories - but are rarely featured in undergraduate curricula. Motivated by the desire to address the curriculum gap, the author of this study developed an inquiry-based laboratory exercise for introducing students to the operating principles and methodology of mass spectrometry-based microbial identification. By requiring students to identify microbes in environmental water samples – a real-life problem with unknown answers – the exercise piqued the students’ interest in learning, while helping to stir their curiosity through an interesting field activity in which they could put on a scientist’s hat in solving a mystery. This synopsis article summarizes a piece of published educational research and expands on the discussion of concepts underlying matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based microbial identification. Herein, the article discusses the relative advantages and disadvantages of the pattern recognition and proteome database search approaches for analyzing mass spectra data. Additionally, the effect of general and tailored sample preparation protocols on identification accuracy is also elaborated. Finally, the pedagogical utility of field- and inquiry-based educational tools is also discussed in greater detail from a post-publication perspective. A full-length synopsis of the work and a structured abstract can be found in the accompanying PDF file, the original article being entitled: “Teaching Microbial Identification with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) and Bioinformatics Tools”.
    Źródło:
    World News of Natural Sciences; 2015, 2; 7-19
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Living Together in the Plankton: A Survey of Marine Protist Symbioses
    Autorzy:
    Anderson, O. Roger
    Powiązania:
    https://bibliotekanauki.pl/articles/763557.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Algal symbionts, bacterial symbionts, marine endosymbiosis, microbial physiology, molecular genetics, plankton ecology
    Opis:
    Our best evidence is that life arose in the marine environment, and over many millennia of evolutionary proliferation, punctuated by occasional massive extinctions, marine protists have developed remarkably elegant and sometimes complex relationships with prokaryotic and eukaryotic symbionts. Current evidence of the range of marine protist taxa possessing symbionts, including their diversity and physiological functional relationships, is reviewed within an ecological context. Some perspectives are presented on potential opportunities for new avenues of research in unraveling the remarkable adaptive value of two or more genetically diverse marine unicellular organisms living in a close structural and physiological relationship.
    Źródło:
    Acta Protozoologica; 2014, 53, 1
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Particle-associated Planktonic Naked Amoebae in the Hudson Estuary: Size-fraction Related Densities, Cell Sizes and Estimated Carbon Content
    Autorzy:
    Anderson, O. Roger
    Powiązania:
    https://bibliotekanauki.pl/articles/763678.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Aquatic food webs, carbon budgets, microbial ecology, micro-habitats, protists
    Opis:
    Naked amoeba densities, sizes, biodiversity and carbon content were examined in relation to two particle size fractions (< 200 μm and > 200 μm) of suspended matter in the water column of the Hudson Estuary at a near-shore location south of the Tappan Zee, Palisades, New York. The densities varied markedly among the two particle fractions, and therefore the mean densities were not significantly different between the larger and smaller particle fractions. In contrast, the mean sizes and mean carbon content were statistically greater on larger size suspended particles compared to smaller size particles. There was a broader size range of amoebae on the larger particles, including very large Cochliopodium, Vannella, Mayorella, and Hartmannella species suggesting a larger biodiversity, also indicated by a larger diversity coefficient for the > 200-μm-particle fraction compared to the < 200-μm-particle fraction, 4.51 and 4.18, respectively. In conclusion, the size of suspended particulates in the water column of near-shore, estuarine habitats may have a significant influence on the composition of naked amoebae communities and their ecological roles, especially the organization of particle-associated microbial food webs.
    Źródło:
    Acta Protozoologica; 2011, 50, 1
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Stimulation of Plant Growth through Interactions of Bacteria and Protozoa: Testing the Auxiliary Microbial Loop Hypothesis
    Autorzy:
    Bonkowski, Michael
    Clarholm, Marianne
    Powiązania:
    https://bibliotekanauki.pl/articles/763461.pdf
    Data publikacji:
    2012
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Protozoa, bacteria, microbial loop, plant growth, priming effect, rhizosphere ecology
    Opis:
    By feeding on bacterial biomass protozoa play an acknowledged role in the liberation of nutrients in the plant rhizosphere. In addition there are suggestions that plants have mechanisms working through changes in root architecture and initiation of active release from soil organic matter, which are used to improve uptake and recirculation of nutrients in the ecosystem. All processes are carried out on a local scale in soil with roots, bacteria and protozoa interacting. The many actors and the small scale of interactions make experimentation difficult. We discuss mistakes, pit falls and misinterpretations and provide suggestions for improvement. Recent methodological progress has opened new exciting avenues for protozoan research. New techniques have already helped to reveal protozoan regulation of cooperation as well as conflict in bacterial communities. These mechanisms in turn affect bacterial functioning and target molecular control points in rhizosphere food webs in relation to plants. Integrating nutritional and regulatory aspects into new concepts of protozoan functioning in soil is a challenging frontier in protozoology.
    Źródło:
    Acta Protozoologica; 2012, 51, 3
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The Metabolic Relationships between Probiotics and Fatty Acids
    Autorzy:
    Dayangaç, A.
    Erdem, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/1031266.pdf
    Data publikacji:
    2017-09
    Wydawca:
    Polska Akademia Nauk. Instytut Fizyki PAN
    Tematy:
    probiotics
    fatty acids
    microbial ecology
    Opis:
    Probiotics are alive bacteria that have a healthy effect on the hosts and are found in large quantities in the soil and foods. The most recommended probiotics as commercial are the some species of Lactobacillus sp., Bifidobacterium sp., and Streptococcus sp. The fatty acids are formed by the colonic gut flora from dietary fibres, which manage to escape the host's enzymatic digestive systems in the small intestine. Dietary fibres that manage to reach the large intestine are available for several bacterial fermentative reactions. The fermentation of the different dietary fibres leads to an increase in concentrations of several short-chain fatty acids, especially butyrate, propionate and acetate, in the lumen of the proximal regions of the large intestine. In this review, we acquainted that the relationship between fatty acids and probiotic bacteria affects some various health ailments.
    Źródło:
    Acta Physica Polonica A; 2017, 132, 3; 816-818
    0587-4246
    1898-794X
    Pojawia się w:
    Acta Physica Polonica A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The role of Bacterial-based Protist Communities in Aquatic and Soil Ecosystems and the Carbon Biogeochemical Cycle, with Emphasis on Naked Amoebae
    Autorzy:
    Anderson, O. Roger
    Powiązania:
    https://bibliotekanauki.pl/articles/763716.pdf
    Data publikacji:
    2012
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Global warming, microbial ecology, microbial respiration rates, microbial trophodynamics, respiratory CO2, soil respiration, terrestrial carbon flow
    Opis:
    Current research is reviewed on aquatic and soil microbial ecology with attention to the fate of organic carbon in bacterial-based protist food webs, including some new data. Particular attention is given to the effects of pulsed sources of low-molecular weight organic sources of carbon on soil respiration, changes in bacterial, nanoflagellate, and naked amoeba C-biomass, and evidence for throughput of carbon in microbial food webs in Arctic and some low-latitude, temperate soil environments. The proportion of pulsed sources of glucose-C that is sequestered in microbial biomass relative to loss as CO2 is examined in laboratory experimental studies, and implications of the research for microbial community dynamics and global warming due to terrestrial sources of respiratory CO2 are discussed.
    Źródło:
    Acta Protozoologica; 2012, 51, 3
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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