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Tytuł:
Arabidopsis thaliana microRNA162 level is posttranscriptionally regulated via splicing and polyadenylation site selection
Autorzy:
Barciszewska-Pacak, Maria
Knop, Katarzyna
Jarmołowski, Artur
Szweykowska-Kulińska, Zofia
Powiązania:
https://bibliotekanauki.pl/articles/1038744.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
miRNA
pri-miRNA
abiotic stress
gene expression
Opis:
Arabidopsis microRNA162 (miRNA162) level regulation was studied under abiotic stresses, such as drought and salinity. The TaqMan® microRNA assay proved that A. thaliana miRNA162 level was elevated under these stresses, confirming its salt and drought responsiveness. The promoter region analyses of A. thaliana miRNA162a and b genes (MIR162a and MIR162b) identified numerous salinity and drought responsive elements. However, our results indicated that Arabidopsis MIR162a was presumably the main locus responsible for the mature ath-miRNA162 accumulation under the stresses tested, and the MIR162b was generally rather weakly expressed, both in control and under the stress conditions. The MIR162a structure was confirmed to be complex and the pri-miRNA162a hairpin structure was shown to span an alternative exon and an intron. The MIR162a transcription generated a few pri-miRNA162a splicing isoforms that could be functional and non-functional. Upon drought and salinity stresses, the regulation of the pri-miRNA162a alternative splicing pattern revealed an increase of a functional pri-miR162a isoform and a preferential distal polyA site selection under the stress conditions. Apart from the potential transcriptional regulation of the miRNA genes (MIRs) expression, the data obtained point to an essential role of posttranscriptional regulation of Arabidopsis microRNA162 level.
Źródło:
Acta Biochimica Polonica; 2016, 63, 4; 811-816
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Barley primary microRNA expression pattern is affected by soil water availability
Autorzy:
Swida-Barteczka, Aleksandra
Kruszka, Katarzyna
Grabowska, Aleksandra
Pacak, Andrzej
Jarmolowski, Artur
Kurowska, Marzena
Szarejko, Iwona
Szweykowska-Kulinska, Zofia
Powiązania:
https://bibliotekanauki.pl/articles/1038746.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
pri-miRNA
miRNA
drought
rehydration
barley genotypes
Opis:
MicroRNAs are short molecules of 21-24 nt in length. They are present in all eukaryotic organisms and regulate gene expression by guiding posttranscriptional silencing of mRNAs. In plants, they are key players in signal transduction, growth and development, and in response to abiotic and biotic stresses. Barley (Hordeum vulgare) is an economically important monocotyledonous crop plant. Drought is the world's main cause of loss in cereal production. We have constructed a high-throughput Real-Time RT-qPCR platform for parallel determination of 159 barley primary microRNAs' levels. The platform was tested for two drought-and-rehydration-treated barley genotypes (Rolap and Sebastian). We have determined changes in the expression of primary microRNAs responding to mild drought, severe drought, and rehydration. Based on the results obtained, we conclude that alteration in the primary microRNA expression is relative to the stress's intensity. Mild drought and rehydration mostly decrease the pri-miRNA levels in both of the tested genotypes. Severe drought mainly induces the primary microRNA expression. The main difference between the genotypes tested was a much-stronger induction of pri-miRNAs in Rolap encountering severe drought. The primary microRNAs respond dynamically to mild drought, severe drought, and rehydration treatments. We propose that some of the individual pri-miRNAs could be used as drought stress or rehydration markers. The usage of the platform in biotechnology is also postulated.
Źródło:
Acta Biochimica Polonica; 2016, 63, 4; 817-824
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Glioblastoma - actual knowledge and future perspectives
Glejak - aktualna wiedza oraz perspektywy
Autorzy:
Bilicki, Dominik
Zbrożek, Mikołaj
Fudalej, Marta
Deptała, Andrzej
Badowska-Kozakiewicz, Anna
Powiązania:
https://bibliotekanauki.pl/articles/2159008.pdf
Data publikacji:
2022-06-30
Wydawca:
Medical Education
Tematy:
glioblastoma multiforme
oncology
miRna
pathogenesis
siRNA
glejak wielopostaciowy
onkologia
miRNA
patogeneza
Opis:
Glioblastoma is the most severe IV-class glioma and therefore the prognosis for patients remains poor despite some improvement in the treatment area. The neurological or psychiatric symptoms especially fast-developing ones should be fully investigated. This article aims to summarize actual knowledge of glioblastoma and present future perspectives. The underlying causes are usually associated with mutations of EGFR, PTEN, IDH1, p53 genes. The MRI scan, MGMT promoter methylation status, GFAP immunohistochemical detection and Karnofsky performance status are valuable diagnostic tools and some other potential biomarkers with high specificity are proposed. The standard of care is surgery and Stupp protocol which is the combination of radiotherapy and chemotherapy with temozolomide. Nevertheless, after remission the treatment possibilities are limited. Many efforts have been devoted to elaborate novel therapeutic strategies using e.g. CAR-T cells, nanoparticles, monoclonal antibodies, miRNA, siRNA or proteasome inhibitors.
Glejak wielopostaciowy klasyfikowany jest do grupy najbardziej złośliwych glejaków o IV stopniu złośliwości ze szczególnie niekorzystnymi prognozami związanymi z jego leczeniem. Pojawienie się u pacjenta szybko postępujących objawów zarówno psychiatrycznych, jak i neurologicznych powinno być zawsze dokładnie zbadane. Artykuł ma na celu zebranie oraz podsumowanie aktualnej wiedzy na temat glejaka oraz zaprezentowanie możliwych perspektyw rozwoju metod diagnostyczno-leczniczych. Rozwój glejaka ma zazwyczaj związek z mutacjami w genach EGFR, PTEN, IDH1 lub p53. Do użytecznych narzędzi diagnostycznych należą m.in. badanie MRI, analiza metylacji promotora genu MGMT oraz immunohistochemiczne oznacznie GFAP. W leczeniu glejaków wielopostaciowych najczęściej stosowanym schematem jest protokół Stuppa bazujący na połączeniu radioterapii z chemioterapią temozolomidem. Niemniej jednak, dalsze możliwości leczenia są limitowane. Zintegrowane wysiłki naukowców są ukierunkowane na poszukiwanie nowych strategii leczenia przy użyciu m.in. teraapii CAR-T, nanocząteczek, przeciwciał monoklonalnych, miRNA, siRNA oraz inhibitorów proteasomów
Źródło:
OncoReview; 2022, 12, 2; 35-44
2450-6125
Pojawia się w:
OncoReview
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Rola wybranych microRNA w procesie neoplazmatycznym
The role of selected microRNAs in the neoplasmatic process
Autorzy:
Świstek, Justyna
Kwiecień, Joanna
Starska, Katarzyna
Powiązania:
https://bibliotekanauki.pl/articles/1033317.pdf
Data publikacji:
2017
Wydawca:
Oficyna Wydawnicza Mediton
Tematy:
geny supresorowe
kancerogeneza
microRNA (miRNA)
onkogeny
Opis:
The work presents the role of selected microRNA molecules (miRNA) and their participation in intracellular mechanisms determining the phenomenon of carcinogenesis and the progression of neoplastic changes. It focuses on the problems related to the role of miRNA as oncogenes or suppressor genes. The authors review the latest literature on the importance of selected microRNAs in the neoplasmatic process, including cancers of the head and neck region.
Praca prezentuje rolę wybranych cząsteczek microRNA (miRNA) i ich udział w mechanizmach wewnątrzkomórkowych determinujących zjawisko kancerogenezy i progresję zmian nowotworowych. Koncentruje się na problematyce związanej z  rolą miRNA jako onkogenów lub genów supresorowych. Autorzy dokonują przeglądu najnowszego piśmiennictwa dotyczącego znaczenia wybranych microRNA w procesie neoplazmatycznym, w tym rakach regionu głowy i szyi.
Źródło:
Otorynolaryngologia - przegląd kliniczny; 2017, 16, 3; 81-87
1643-658X
Pojawia się w:
Otorynolaryngologia - przegląd kliniczny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Regulatory RNAs in Planarians
Autorzy:
Pawlicka, Kamila
Perrigue, Patrick
Barciszewski, Jan
Powiązania:
https://bibliotekanauki.pl/articles/1038722.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
planarians
RNAi
miRNA
regeneration
stem cells
Opis:
The full scope of regulatory RNA evolution and function in epigenetic processes is still not well understood. The development of planarian flatworms to be used as a simple model organism for research has shown a great potential to address gaps in the knowledge in this field of study. The genomes of planarians encode a wide array of regulatory RNAs that function in gene regulation. Here, we review planarians as a suitable model organism for the identification and function of regulatory RNAs.
Źródło:
Acta Biochimica Polonica; 2016, 63, 4; 681-686
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Deciphering the soybean molecular stress response via high-throughput approaches
Autorzy:
Tyczewska, Agata
Gracz, Joanna
Kuczyński, Jakub
Twardowski, Tomasz
Powiązania:
https://bibliotekanauki.pl/articles/1038715.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
soybean
transcriptome
miRNA
proteome
stress conditions
Opis:
As a result of thousands of years of agriculture, humans had created many crop varieties that became the basis of our daily diet, animal feed and also carry industrial application. Soybean is one of the most important crops worldwide and because of its high economic value the demand for soybean products is constantly growing. In Europe, due to unfavorable climate conditions, soybean cultivation is restricted and we are forced to rely on imported plant material. The development of agriculture requires continuous improvements in quality and yield of crop varieties under changing or adverse conditions, namely stresses. To achieve this goal we need to recognize and understand the molecular dependencies underlying plant stress responses. With the advent of new technologies in studies of plant transcriptomes and proteomes, now we have the tools necessary for fast and precise elucidation of desirable crop traits. Here, we present an overview of high-throughput techniques used to analyze soybean responses to different abiotic (drought, flooding, cold stress, salinity, phosphate deficiency) and biotic (infections by F. oxysporum, cyst nematode, SMV) stress conditions at the level of the transcriptome (mRNAs and miRNAs) and the proteome.
Źródło:
Acta Biochimica Polonica; 2016, 63, 4; 631-643
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
MicroRNA-mediated regulation of flower development in grasses
Autorzy:
Smoczynska, Aleksandra
Szweykowska-Kulinska, Zofia
Powiązania:
https://bibliotekanauki.pl/articles/1038724.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
miRNA
grasses
flower development
ABCDE model
Opis:
Flower structure in grasses is very unique. There are no petals or sepals like in eudicots but instead flowers develop bract-like structures - palea and lemma. Reproductive organs are enclosed by round lodicule that not only protects reproductive organs but also plays an important role during flower opening. The first genetic model for floral organ development was proposed 25 years ago and it was based on the research on model eudicots. Since then, studies have been carried out to answer the question whether this model could be applicable in the case of monocots. Genes from all classes found in eudicots have been also identified in genomes of such monocots like rice, maize or barley. What's more, it seems that miRNA-mediated regulation of floral organ genes that was observed in the case of Arabidopsis thaliana also takes place in monocots. MiRNA172, miRNA159, miRNA171 and miRNA396 regulate expression of floral organ identity genes in barley, rice and maize, affecting various features of the flower structure, ranging from formation of lemma and palea to the development of reproductive organs. A model of floral development in grasses and its genetic regulation is not yet fully characterized. Further studies on both, the model eudicots and grasses, are needed to unravel this topic. This review provides general overview of genetic model of flower organ identity specification in monocots and it's miRNA-mediated regulation.
Źródło:
Acta Biochimica Polonica; 2016, 63, 4; 687-692
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of microRNA in metastatic colorectal cancer and its significance in cancer prognosis and treatment
Autorzy:
Tokarz, Paulina
Blasiak, Janusz
Powiązania:
https://bibliotekanauki.pl/articles/1039629.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
prognosis
colorectal cancer
metastasis
prediction
therapy
miRNA
Opis:
microRNAs (miRNAs) are small, non-coding RNA molecules that regulate gene expression by targeting specific mRNAs. microRNAs play a role in several physiological processes in the cell, including migration, proliferation, differentiation and apoptosis. Apart from their role in regular metabolism, abnormal profiles of miRNA expression accompany cancer transformation, including colorectal cancer (CRC) metastasis. microRNAs may play a role in each phase of CRC metastasis including angiogenesis, invasion, intravasation, circulation, extravasation and metastatic colonization. microRNA levels may serve as a predictive CRC marker, which was confirmed by the serum level of miR-29a targeting KLF4, a marker of cell stemness, and the plasma level of miR-221 down-regulating c-Kit, Stat5A and ETS1, which are signal transducers and transcription factor, respectively. In turn, the level of miR-143 in CRC cells decreasing the amount of MACC1 (metastasis-associated in colon cancer-1) and oncogenic KRAS protein, may be utilized as a prognostic marker. Also, single nucleotide polymorphisms of genes encoding miRNAs, including miR-423 and miR-608, which correlate with tumor recurrence, may be useful as diagnostic, prognostic and predictive indicators in CRC metastasis. Pre-miR-34a and pre-miR-199a decreased the level of Axl, a tyrosine-protein kinase receptor, so they can be considered as drugs in antimetastatic therapy. On the other hand, miR-222 targeting ADAM-17, a disintegrin and metalloproteinase, and miR-328 interacting with ABCG2, an ABC transporter, may overcome drug resistance of cancer cells. microRNAs may be considered in wide-range application to facilitate CRC metastasis diagnosis, prognosis, prediction and therapy, however, further clinical, epidemiological and in vitro studies should be conducted to verify their utility.
Źródło:
Acta Biochimica Polonica; 2012, 59, 4; 467-474
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
CAMPTOTHECIN INHIBITS MIGRATION, INVASION AND CLONOGENIC PROPERTY OF LIVER CANCER CELLS BY MODULATING MICRORNA EXPRESSION
Autorzy:
Liu, Zhenzhong
Wu, Song
Li, Xiaoqian
Powiązania:
https://bibliotekanauki.pl/articles/895460.pdf
Data publikacji:
2020-04-29
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
Hepatocellular Carcinoma
cell proliferation
cell cycle
Camptothecin
miRNA
Opis:
Camptothecin (CPT), an alkaloid natural product, extracted from Camptotheca acuminata bark, has been reported to have potential antitumor activity in diverse cancers. MicroRNAs (MiRNAs) are a class of short, non-coding RNAs that plays a crucial role in the normal physiology by attenuating translation. Here, we showed that the CPT modulates the expression of miRNAs in hepatocellular carcinoma cells (HCC). Microarray analysis reveals that CPT modulates the expression of as many as 39 miRNAs in HCC cells (Huh7), 27 miRNAs were downregulated whereas 12 miRNAs were upregulated. miR-16 is the key miRNA upregulated by CPT and targets key prosurvival proteins (MMP-2, MMP-9 and cyclin D1). Our results demonstrate that CPT is inhibiting cell viability of HCC cells significantly when compared with the untreated cells. Wound healing and colony formation assay confirm inhibition of cell migration and clonogenic property of Huh7 cells respectively, upon the dose-dependent treatment of CPT. Furthermore, the Boyden chamber assay analysis revealed a significant inhibition of number of invasive cells in CPT treated cells with comparison to untreated Huh7 cells. Mechanistically, CPT upregulates miR-16 expression which targets MMP-2, MMP-9, cyclin D1 downregulation and subsequently upregulates the expression of E-cadherin, TIMP1, p21, and p27, thereby inhibits cell migration, invasion and clonogenic property of HCC cells. In summary, CPT treatment in Huh7 cells decreases cell viability and upregulates miR-16 expression, which results in inhibition of cell migration, invasion and clonogenic property of cells, by decreasing MMP-2, MMP-9, cyclin D1 and increasing the expression of cell cycle-regulated proteins p21 and p27.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2020, 77, 2; 295-304
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New functionality of RNAComposer: an application to shape the axis of miR160 precursor structure
Autorzy:
Antczak, Maciej
Popenda, Mariusz
Zok, Tomasz
Sarzynska, Joanna
Ratajczak, Tomasz
Tomczyk, Katarzyna
Adamiak, Ryszard
Szachniuk, Marta
Powiązania:
https://bibliotekanauki.pl/articles/1038732.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
RNA 3D structure prediction
RNAComposer
plant miRNA precursor
Opis:
RNAComposer is a fully automated, web-interfaced system for RNA 3D structure prediction, freely available at http://rnacomposer.cs.put.poznan.pl/ and http://rnacomposer.ibch.poznan.pl/. Its main components are: manually curated database of RNA 3D structure elements, highly efficient computational engine and user-friendly web application. In this paper, we demonstrate how the latest additions to the system allow the user to significantly affect the process of 3D model composition on several computational levels. Although in general our method is based on the knowledge of secondary structure topology, currently the RNAComposer offers a choice of six incorporated programs for secondary structure prediction. It also allows to apply a conditional search in the database of 3D structure elements and introduce user-provided elements into the final 3D model. This new functionality contributes to a significant improvement of the predicted 3D model reliability and it facilitates a better model adjustment to the experimental data. This is exemplified based on the RNAComposer application for modelling of the 3D structures of precursors of the miR160 family members.
Źródło:
Acta Biochimica Polonica; 2016, 63, 4; 737-744
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
AmiRNA Designer - new method of artificial miRNA design
Autorzy:
Mickiewicz, Agnieszka
Rybarczyk, Agnieszka
Sarzynska, Joanna
Figlerowicz, Marek
Blazewicz, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/1038843.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
artificial miRNA
RNAi
gene regulation
sequence specific
thermodynamic profiles
Opis:
MicroRNAs (miRNAs) are small non-coding RNAs that have been found in most of the eukaryotic organisms. They are involved in the regulation of gene expression at the post-transcriptional level in a sequence specific manner. MiRNAs are produced from their precursors by Dicer-dependent small RNA biogenesis pathway. Involvement of miRNAs in a wide range of biological processes makes them excellent candidates for studying gene function or for therapeutic applications. For this purpose, different RNA-based gene silencing techniques have been developed. Artificially transformed miRNAs (amiRNAs) targeting one or several genes of interest represent one of such techniques being a potential tool in functional genomics. Here, we present a new approach to amiRNA*design, implemented as AmiRNA Designer software. Our method is based on the thermodynamic analysis of the native miRNA/miRNA* and miRNA/target duplexes. In contrast to the available automated tools, our program allows the user to perform analysis of natural miRNAs for the organism of interest and to create customized constraints for the design stage. It also provides filtering of the amiRNA candidates for the potential off-targets. AmiRNA Designer is freely available at http://www.cs.put.poznan.pl/arybarczyk/AmiRNA/.
Źródło:
Acta Biochimica Polonica; 2016, 63, 1; 71-77
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
miRNA zaangażowane w proces starzenia i kiełkowania nasion
miRNAs involved in the seed ageing and germination process
Autorzy:
Puchta, Marta
Powiązania:
https://bibliotekanauki.pl/articles/2199377.pdf
Data publikacji:
2020-10-22
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
miRNA
starzenie nasion
żywotność
kiełkowanie
seed aging
vitality
germination
Opis:
Starzenie nasion jest procesem prowadzącym do nieodwracalnych zmian powodujących utratę ich wartości siewnej w czasie. W proces ten zaangażowanych jest wiele wzajemnie powiązanych zmian molekularnych, biochemicznych, fizjologicznych oraz metabolicznych. W związku z degradacją środowiska naturalnego wymagana jest intensywna ochrona gatunkowa roślin. Zachowanie zdolności do kiełkowania nasion odgrywa kluczową rolę w ochronie bioróżnorodności gatunkowej. Przeprowadzone dotychczas badania wskazują na udział miRNA w dynamicznym procesie kiełkowania nasion. Jednak niewiele wiadomo na temat zagadnień dotyczących regulacji miRNA i ich genów docelowych, które są potencjalnie ważnymi czynnikami przyczyniającymi się do starzenia nasion podczas długotrwałego przechowywania.Praca obejmuje przegląd wybranej literatury dotyczącej badania roli miRNA w regulacji procesów zaangażowanych w starzenie i kiełkowanie nasion.
The ageing of seeds is a process that leads to irreversible changes that cause the loss of seed value over time. Many interrelated molecular, biochemical, physiological and metabolic processes are involved in it. The degradation of the natural environment requires intensive plant species protection. Preserving germination capacity plays a key role in protecting the biodiversity damaged by genetic erosion. The studies conducted so far show that miRNAs are involved in the dynamic germination process of seeds. However, little is known about the state of specific miRNA regulations and their targets. These, are potentially important factors contributing to the ageing of seeds during long-term storage.This review includes literature review of research concerning the role of miRNA in the regulation of processes leading to seed ageing and germination.
Źródło:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2020, 290; 21-26
0373-7837
2657-8913
Pojawia się w:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
miRNA Multiplayers in glioma. From bench to bedside
Autorzy:
Rolle, Katarzyna
Powiązania:
https://bibliotekanauki.pl/articles/1038962.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cancer
glioblastoma multiforme (GBM)
miRNA
anti-cancer therapy
diagnosis
prognosis
Opis:
Glioblastoma multiforme (GBM) is the most common type of malignant gliomas, characterized by genetic instability, intratumoral histopathological variability and unpredictable clinical behavior. Disappointing results in the treatment of gliomas with surgery, radiation and chemotherapy have fuelled a search for a new therapeutic targets and treatment modalities. A novel small non-coding RNA molecules, microRNAs (miRNAs), appear to represent one of the most attractive target molecules contributing to the pathogenesis of various types of tumors. They play crucial roles in tumorigenesis, angiogenesis, invasion and apoptosis. Some miRNAs are also associated with clinical outcome and chemo- and radiotherapy resistance. Moreover, miRNA have the potential to affect the responses to molecular-targeted therapies and they also might be associated with cancer stem cell properties, affecting tumor maintenance and progression. The expression profiles of miRNAs are also useful for subclassification of GBM, what underscores the heterogeneity of diseases that all share the same WHO histopathological grade. Importantly, molecular subtypes of GBM appear to correlate with clinical phenotypes, tumor characteristic and treatment outcomes. miRNAs are then biological markers with possible diagnostic and prognostic potential. They could also serve as one of the promising treatment targets in human glioblastoma.
Źródło:
Acta Biochimica Polonica; 2015, 62, 3; 353-365
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hsa-miR-331-3p inhibits VHL expression by directly targeting its mRNA 3-UTR in HCC cell lines
Autorzy:
Cao, Yiyi
Zhang, Jinnan
Xiong, Dongmei
Wang, Dan
Wu, Ting
Huang, Ailong
Tang, Hua
Powiązania:
https://bibliotekanauki.pl/articles/1039137.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
HCC
HBV
miRNA
Hsa-miR-331-3p and VHL
Opis:
Dysregulation of miRNA is widely involved in human cancers, including hepatocellular carcinoma (HCC). Array data for miRNAs indicated that miR-331-3p might be one of the disorderly expressed miRNAs in HCC cell lines, but the function of miR-331-3p in HCC remains unclear. In this study, quantitative real time polymerase chain reaction (qRT-PCR) results indicated that miR-331-3p was up-regulated in HepG2.2.15 cells, Ad-HBV-HepG2 cells and pCH9/3091transfected SMMC7721 cells compared with their control group, respectively. miRNA target prediction software was used, and VHL was found to be one of the target genes of miR-331-3p. qRT-PCR and western blot analysis indicated VHL expression was decreased when miR-331-3p was over-expressed and increased when miR-331-3p was inhibited in SMMC7721 cells. The luciferase reporter activity was inhibited in SMMC7721 cells when co-transfected with miR-331-3p expression vector and VHL 3'-UTR wild type vector and increased in HepG2.2.15 transfected with miR-331-3p inhibitor compared to its control group respectively. When co-transfected with miR-331-3p expression vector and VHL 3'-UTR mutated type vector in SMMC7721 cells the luciferase reporter activity was recovered. All of these results show that HBV up-regulated miR-331-3p expression in HCC cell lines and miR-331-3p could inhibit VHL expression by directly targeting its 3'-UTR. This provided useful information in exploring the mechanism of HCC induced by HBV infection.
Źródło:
Acta Biochimica Polonica; 2015, 62, 1; 77-82
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Udział niskocząsteczkowych regulatorowych RNA (siRNA i miRNA) w regulacji szlaku transdukcji sygnału auksyn
Involvement of low molecular weight regulatory RNAs (siRNAs and miRNAs) in regulation of auxin signal transduction pathway
Autorzy:
Glazińska, Paulina
Kulasek, Milena
Grzeca, Michał
Wojciechowski, Waldemar
Marciniak, Katarzyna
Wilmowicz, Emilia
Kopcewicz, Jan
Powiązania:
https://bibliotekanauki.pl/articles/1034521.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Przyrodników im. Kopernika
Tematy:
auksyna
miRNA
szlak transdukcji sygnału auksynowego
regulacja ekspresji genów
sRNA
siRNA
ta-siRNA
Opis:
Auksyna wpływa na większość procesów wzrostu i rozwoju roślin. Szlak transdukcji sygnału auksyn tworzony jest przez komponenty białkowe, z których kluczowe są: receptory z rodziny TAAR (TIR1 oraz AFB1-3), represory odpowiedzi na auksynę AUX/IAA i czynniki transkrypcyjne AUXIN RESPONSE FACTOR (ARF). Aktywność genów kodujących niektóre elementy tego szlaku jest regulowana przez niskocząsteczkowe regulatorowe RNA, miRNA (micro RNA), i siRNA (short-interfering RNA), endogenne, niekodujące małe RNA (small RNA, sRNA) o długości 20-25 nukleotydów, różniące się sposobem powstawania (prekursorowymi molekułami i szlakami syntezy) oraz funkcją. Sekwencje TIR1 i AFB1-3 zawierają miejsce docelowe dla miR393 i drugorzędowe dla siTAAR. Transkrypt genu IAA28 jest celem dla miR847. Ekspresja genów: ARF10, ARF16 i ARF17 podlega bezpośredniej kontroli przez miR160, ARF6 i ARF8 przez miR167, natomiast ekspresja ARF2-4 podlega regulacji przez miR390 za pośrednictwem ta-siRNA pochodzącego z locus TAS3. sRNA wpływają przede wszystkim na lokalizację tkankową i czasową opisanych elementów szlaku auksynowego.
Auxin affects almost all of the growth and development processes in plants. The auxin signal transduction pathway involves a number of proteins, among which the key elements are: TAAR auxin receptors (TIR1 and AFB1-3), AUX/IAA auxin response repressors and Auxin Response Factor (ARF) transcription factors. The activity of genes encoding some components of this pathway is affected by regulatory low-molecular-weight RNAs - miRNA (micro RNA) and siRNA (short-interfering RNA) - endogenous non-coding 20-25 nucleotides long small RNA (sRNA), differing in the way of formation (precursor molecules and biosynthesis pathways) and function. TIR1 and AFB1-3 contain miR393 target sequence and siTAAR secondary target site. IAA28 transcripts are targeted by miR847. Expression of ARF10, ARF16 i ARF17 is directly controlled by miR160, ARF6 and ARF8 by miR167, and ARF2-4 indirectly by miR390 through TAS3-derived ta-siRNAs. sRNAs influence primarily the tissue and temporal localization of described components of the auxin signal transduction pathway.
Źródło:
Kosmos; 2016, 65, 3; 399-410
0023-4249
Pojawia się w:
Kosmos
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 18

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