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    Wyświetlanie 1-3 z 3
    Tytuł:
    Mass spectrometry identification of membrane-bound respiratory nitrate reductase from Bradyrhizobium sp. (Lupinus)
    Autorzy:
    Polcyn, Władysław
    Powiązania:
    https://bibliotekanauki.pl/articles/1040683.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    immunoblot screening
    membrane-bound nitrate reductase [EC 1.7.99.4]
    enzyme purification
    Bradyrhizobium sp. (Lupinus)
    rhizobia
    mass spectrometry sequencing
    Opis:
    Respiratory nitrate reductase (NR) from Bradyrhizobium sp. (Lupinus) USDA 3045 has biochemical properties of the membrane-bound NR type. However, in the completely sequenced rhizobium genomes only genes for the periplasmic type of dissimilatory NR were found. Therefore purification and identification of the enzyme by tandem mass spectrometry (MS/MS) was undertaken. MS/MS spectra representing 149 unique tryptic peptides derived from purified 137-kDa subunit matched the NCBInr-deposited NarG sequences. MS/MS sequencing of two other SDS/PAGE bands (65- and 59-kDa) identified them as derivatives of the NarH-type protein. Applying additional validation criteria, 73% of the sequence of the NarG subunit (902 aa) and 52% of NarH sequence (266 aa) was assembled (UniProt KB acc. no. P85097 and P85098). This is the first unambiguous identification of an active NarGH-like NR in rhizobia. Moreover, arguments are provided here for the existence of a functional enzyme of this type also among other rhizobial species, basing on immunoblot screening and the presence of membrane-associated NR-active electrophoretic forms.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 4; 753-760
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mass spectrometric analysis of head-to-tail connected cyclic peptides.
    Autorzy:
    Macht, Marcus
    Pelzing, Matthias
    Palloch, Petra
    Sauerland, Volker
    Volz, Jürgen
    Powiązania:
    https://bibliotekanauki.pl/articles/1044055.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    electrospray
    post source decay
    MALDI
    mass spectrometry
    peptide sequencing
    Opis:
    Tandem mass spectrometry is an extremly useful tool for high sensitive sequence identification of peptides. In the case of cyclic peptides fragmentation can easily be performed for sequence analysis. However, analysis is usually tedious due to the lack of a defined beginning and end of the sequence. Since cyclic peptides are a highly interesting class of compounds especially for the pharmaceutical industry, ways have to be found to identify their structures. In this work we demonstrate how software and dedicated analytical strategies can be used for detailed analysis of these substances.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 4; 1109-1112
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Advanced methods of bacteriological identification in a clinical microbiology laboratory
    Autorzy:
    Zukowska, M.E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2098275.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    clinical microbiology
    molecular method
    modern method
    multiplex polymerase chain reaction
    real-time polymerase chain reaction
    next generation sequencing
    MALDI-TOF mass spectrometry
    Opis:
    Introduction and objective. Conventional, culture-based methods of bacterial identification and drug-susceptibility testing are considered the gold standard in medical microbiology. In recent years, classical microbiological methods have been supplemented with modern analytical and molecular methods. The aim of the review was to discusses the methods which have been permanently adapted to bacteriological microbiological diagnostics. Abbreviated description of the state of knowledge. Currently, PCR, as well as other nucleic acid amplification tests and sequencing techniques, are part of the standard repertoire of microbiological diagnostics. With regard to the quality and speed of pathogen identification, the introduction of mass spectrometry techniques into routine microbiological diagnostics work-up has been revolutionary. Within a short time in many laboratories, Matrix-Assisted Laser Desorption/Ionisation – Time of Flight Mass Spectrometry (MALDI TOF MS) systems have almost completely replaced conventional biochemical pathogen identification. Conclusions. Microbiological diagnostics is an indispensable element of a targeted therapy. The techniques used in the laboratory depend primarily on the laboratory’s apparatus, the costs of the analysis, as well as the sensitivity and specificity of a method. However, regardless of the culture-based methods universality, advanced techniques have permanently established themselves in diagnostics. Confident information about the detected organism and treatment possibilities in a combination with the clinical context are conducive to successful therapy. Although modern methods still require validation and close collaboration between clinicians, microbiologists and bioinformaticians, these methods, once deemed to be the future, have already arrived.
    Źródło:
    Journal of Pre-Clinical and Clinical Research; 2021, 15, 2; 68-72
    1898-2395
    Pojawia się w:
    Journal of Pre-Clinical and Clinical Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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