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Tytuł:
Liposomal drug delivery, a novel approach: PLARosomes.
Autorzy:
Kozubek, Arkadiusz
Gubernator, Jerzy
Przeworska, Ewa
Stasiuk, Maria
Powiązania:
https://bibliotekanauki.pl/articles/1044303.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
drugs
liposomes
vesicles
amphiphiles
Opis:
Almost from the time of their rediscovery in the 60's and the demonstration of their entrapment potential, liposomal vesicles have drawn attention of researchers as potential carriers of various bioactive molecules that could be used for therapeutic applications in humans and animals. Several commercial liposome-based drugs have already been discovered, registered and introduced with great success on the pharmaceutical market. However, further studies, focusing on the elaboration of more efficient and stable amphiphile-based vesicular (or non-viral) drug carriers are still under investigation. In this review we present the achievements of our group in this field. We have discovered that natural amphiphilic dihydroxyphenols and their semisynthetic derivatives are promising additives to liposomal lipid compositions. The presence of these compounds in lipid composition enhances liposomal drug encapsulation, reduces the amount of the lipid carrier necessary for efficient entrapment of anthracycline drugs by a factor of two, stabilizes liposomal formulation of the drug (both in suspension and in a lyophilized powder), does not influence liposomal fate in the blood circulation system and benefits from other biological activities of their resorcinolic lipid modifiers.
Źródło:
Acta Biochimica Polonica; 2000, 47, 3; 639-649
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Impact of humic acids on EYL liposome membranes : ESR method
Autorzy:
Pytel, B.
Filipiak, A.
Pisarek, I.
Olchawa, R.
Man, D.
Powiązania:
https://bibliotekanauki.pl/articles/146924.pdf
Data publikacji:
2015
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
EYL liposomes
ESR method
humic substances
Opis:
In this paper, the effects of model (commercial) and natural (extracted from peat) humic substances on the membrane of liposomes formed with egg yolk lecithin (EYL) are presented. In our research, mass concentrations of fulvic and humic acids were used, which in relation to lecithin varied from 0% to 13%. To study membrane fluidity, electron spin resonance (EPR) was used with two spin probes, penetrating various regions of the lipid bilayer. The effects of model and natural humic substances (humic acids – HAs and fulvic acids – FAs) on the lipid membrane in different regions were researched: the lipid-water interphase, and in the middle of the lipid bilayer. It was shown that FA and HA impact the fluidity of liposome membranes in different ways. Increased mass concentrations of HAs decreased membrane fluidity in both acids: extracted from peat and the model. However, increased mass concentration of FAs extracted from peat, decreased membrane fluidity in the surface region, at the same time stiffening the central part of the bilayer. Increasing the concentration of FAs extracted from peat had the opposite effect when compared to model FA. This effect may be related to the complexation of xenobiotics present in the soil environment and their impact on biological membranes.
Źródło:
Nukleonika; 2015, 60, No. 3, part 1; 455-459
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phenolic Extracts from Vaccinium corymbosum L. Loaded in Microemulsions and Liposomes as Enhancers of Olive Oil Oxidative Stability
Autorzy:
Liović, Nikolina
Bošković, Perica
Drvenica, Ivana
Režek Jambrak, Anet
Dropulić, Ana Marija
Krešić, Greta
Nedović, Viktor
Bugarski, Branko
Zorić, Zoran
Pedisić, Sandra
Bilušić, Tea
Powiązania:
https://bibliotekanauki.pl/articles/1369546.pdf
Data publikacji:
2019
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
phenolics blueberry
microemulsions
liposomes
Antioxidant
ultrasound
Opis:
Natural phenolic compounds are recognized as bioactive ingredients in food but can also have a role as effective alternatives to synthetic antioxidants in stability improvement of foods prone to oxidation, such as edible oils. This study aimed at the preparation and HPLC-DAD characterization of phenolic extracts from Vaccinium corymbosum L. (raw, pasteurized, freeze-dried and treated with high-intensity ultrasound), and at testing their antioxidant potential in the prevention of olive oil oxidation in the native state and encapsulated into microemulsions and liposomes systems. Water-in-oil structured microemulsions used in this study were prepared using mechanical, ultrasonic, and high pressure homogenization. Liposomes with the average size of 589.1±2.9 nm were produced with the proliposome method using commercially available phosphatidylcholine – Phospolipon 90G. The obtained results showed significant prolongation of the oxidative stability of extra virgin olive oil enriched with encapsulated blueberry phenolic extracts than with native phenolic extracts, regardless of the method used for blueberry processing. Phenolic extracts encapsulated in microemulsions had a stronger effect on the prolongation of olive oil oxidative stability in comparison with the extracts encapsulated in liposomes. The average prolongation rate of oxidative stability was 45.65% by phenolic extracts encapsulated in microemulsions prepared by mechanical homogenization (p=0.012), and 58.72% by microemulsions prepared by ultrasound homogenization (p=0.011). Phenolic extracts encapsulated in microemulsions prepared by high pressure homogenization had no effect on oil oxidative stability prolongation.
Źródło:
Polish Journal of Food and Nutrition Sciences; 2019, 69, 1; 23-33
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Combination of combretastatin A4 phosphate and doxorubicin-containing liposomes affects growth of B16-F10 tumors
Autorzy:
Mitrus, Iwona
Sochanik, Aleksander
Cichoń, Tomasz
Szala, Stanisław
Powiązania:
https://bibliotekanauki.pl/articles/1040651.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
CA4P
doxorubicin
liposomes
combined therapy
Opis:
The study aimed to check the effectiveness of anticancer therapy combining a vascular-disruptive drug (combretastatin phosphate, CA4P) and a liposomal formulation of a chemotherapeutic (doxorubicin). CA4P was synthesized in our laboratory according to a previously described procedure. The antivascular drug and long-circulating doxorubicin-loaded liposomes were used to treat B16-F10 murine melanoma experimental tumors. Seventy-four hours after drug administration, a decrease in the number of tumor blood vessels was apparent and necrotic areas within tumors were visible. Combination therapy consisting of alternate administrations of CA4P and liposomal doxorubicin yielded greater inhibition of tumor growth than monotherapies alone. The best therapeutic results were obtained with the antivascular drug administered intratumorally every second day at 50 mg/kg body mass. In the case of combined therapy, the best results were obtained when the vascular-disruptive agent (CA4P) and the antineoplastic agent (liposomal doxorubicin) were administered in alternation.
Źródło:
Acta Biochimica Polonica; 2009, 56, 1; 161-165
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study of antioxidant activity of liposomal forms of quercetin and curcumin in ischemic heart disease
Autorzy:
Pylypenko, D.
Gorbach, T.
Krasnopolsky, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2096332.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
curcumin
antioxidant
quercetin
liposomes
oxidative stress markers
Opis:
Quercetin and curcumin are plant polyphenolic antioxidants with proven pharmacological efficacy. Their use is, however, limited due to low bioavailability and oral administration route. The encapsulation of lipophilic compounds in liposomes enables to increase their bioavailability and to create an injectable form. The present study aimed to comparatively investigate the antioxidant activity of a complex liposomal preparation containing two lipophilic antioxidants (quercetin and curcumin) and their monopreparations in liposomal forms. This study was conducted on Wistar line rats with experimental model of ischemic heart disease. Oxidative stress markers such as total antioxidant activity, malondialdehyde, and peroxidized proteins were analyzed in blood serum and cardiac tissue. Ischemic heart disease is accompanied with lipid peroxidation and changes in the activity of the antioxidant system. The obtained results demonstrated the antioxidant activity of monopreparations of curcumin and quercetin and their complex in liposomal forms. Quercetin and curcumin showed different antioxidant activities in terms of oxidative stress markers. The complex of the two antioxidants showed the synergistic effect of their lipophilic compounds in liposomal forms, which led to the normalization of test parameters according to the level of the control sample.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 4; 273-282
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Licensed liposomal vaccines and adjuvants in the antigen delivery system
Autorzy:
Krasnopolsky, Yuriy
Pylypenko, Daria
Powiązania:
https://bibliotekanauki.pl/articles/16648151.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
vaccine
mRNA
liposomes
adjuvant
virosoma
lipid nanoparticles
Opis:
Liposomes (LSs) are promising nanoparticles with unique properties such as controlled nanosize, large surface area, increased reactivity, and ability to undergo modification. Worldwide, licensed liposomal forms of antibiotics, hormones, antioxidants, cytostatics, ophthalmic drugs, etc., are available on the pharmaceutical market. This review focuses on the adjuvant properties of LSs in the production of vaccines (VACs). LS-VACs have the following advantages: antigens with low immunogenicity can become highly immunogenic; LSs can include both hydrophilic and hydrophobic antigens; LSs allow to achieve a prolonged specific action of antibodies; and LSs reduce the toxicity and pyrogenicity of encapsulated antigens and adjuvants. The immune response is influenced by the composition of the liposomal membrane, physicochemical characteristics of lipids, antigen localization in LSs, interaction of LSs with complement, and a number of proteins, which leads to opsonization. The major requirements for adjuvants are their ability to enhance the immune response, biodegradability, and elimination from the organism, and LSs fully meet these requirements. The effectiveness and safety of LSs as carriers in the antigen delivery system have been proven by the long-term clinical use of licensed vaccines against hepatitis A, influenza, herpes zoster, malaria, and COVID-19.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2022, 103, 4; 409-423
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Liposomy jako nośniki substancji aktywnych przenoszonych w głąb skóry
Liposomes as carriers for the delivery of active substances to the skin
Autorzy:
Goik, U.
Załębska-Żyłka, I.
Pietrzycka, A.
Powiązania:
https://bibliotekanauki.pl/articles/286040.pdf
Data publikacji:
2015
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
liposomy
stratum corneum
dyfuzja przezskórna
liposomes
transdermal diffusion
Opis:
Od bardzo dawna skóra jest miejscem aplikacji kosmetyków i leków dermatologicznych. Obecnie można zauważyć coraz większe zainteresowanie preparatami aplikowanymi na skórę w celu leczenia, czy uzyskania dobrego efektu kosmetycznego. Działanie leków podawanych na powierzchnię skóry nie ogranicza się tylko do jej obszarów, ale dzięki wchłanianiu substancji leczniczych przez nią lek może dotrzeć do głębiej położonych tkanek lub do krwiobiegu. Podobnie preparaty kosmetyczne mogą działać w obrębie warstw naskórka jak również skóry właściwej oraz przedostawać się do krwiobiegu. Liposomy są to pęcherzyki, które otoczone są podwójną warstwą lipidową. Powstają one samoistnie z fosfolipidów w środowisku wodnym i są wypełnione niewielką ilością roztworu wodnego, z którego powstają. Posiadają wiele pozytywnych właściwości na przykład: mogą przenosić zarówno wodę jak i rozpuszczalne w lipidach leki, mogą zawierać micro i makro molekuły, zapewniają długotrwałe uwalnianie i ukierunkowane dostarczanie leku oraz docelowe dostarczenie leku w środowisku nieprzyjaznym, stabilizują biodegradowalne leki i związki aktywne zapewniając ochronę przed utlenianiem, poprawiają stabilizację protein i mogą być aplikowane do organizmu poprzez wiele dróg dostarczania. Liposomy znalazły swoje zastosowanie w wielu dziedzinach, takich jak biotechnologia, mikrobiologia oraz w przemyśle kosmetycznym, farmaceutycznym i spożywczym. Ponadto osiągnięcia nanomedycyny są przełomowe w przenoszeniu leków, a wszechstronność liposomów spowodowała wzrost ich użycia w wielu dziedzinach medycyny, takich jak diagnoza i terapia.
Since ancient times cosmetics and dermatological medicines have been applied to the skin. Presently growing interest in topical administration in order to achieve therapeutic or good cosmetic effects can be noticed. Effectiveness of medicines topically applied is not limited only to the skin, but due to the absorption of medical substances, the drug can reach subdermal tissues or circulatory system. Similarly, active substances in cosmetic can act both within epidermis and dermis layers, and can enter the bloodstream. Liposomes are vesicles which are surrounded by a lipid bilayer. They are created spontaneously from phospholipids in aqueous environment and they are filled with a small amount of aqueous solution of hydrophilic active substances. Incorporation of micro and macro molecules in liposomes provides stabilization, constant release during the time unit and targeted drug delivery in hostile environment. Biodegradable drugs protected from oxidation improve protein stabilization and can be administered through various routes. Liposomes are used in numerous applications, including cosmetics and biotechnology, microbiology, and also pharmaceutical and food industry. Moreover, nanomedicine developments are crucial for carrying drugs, and liposomes versatility leads to increase in their application in medical fields, like diagnosis and therapy.
Źródło:
Engineering of Biomaterials; 2015, 18, 130; 27-39
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Stabilność liposomowych nośników leków podczas rozpylania za pomocą wybranych urządzeń nebulizacyjnych
Stability of liposomal drug carriers during atomization in selected medical nebulizers
Autorzy:
Jabłczyńska, K.
Sosnowski, T. R.
Powiązania:
https://bibliotekanauki.pl/articles/2072172.pdf
Data publikacji:
2013
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
atomizacja
liposomy
enkapsulacja
inhalatory
atomization
liposomes
encapsulation
inhalers
Opis:
Badano wpływ metody atomizacji na trwałość rozpylanych liposomów jako nośników leków inhalacyjnych. Monitorując wielkość emitowanych kropel i stężenie enkapsulowanego trasera wykazano, że silne naprężenia towarzyszące atomizacji w nebulizatorze pneumatycznym silnie destabilizują rozpylany koloid. Korzystniejsze warunki atomizacji panują w inhalatorze typu VM, gdzie nie stwierdzono istotnej dezintegracji liposomów. Wyniki pozwalają na racjonalny dobór techniki atomizacji koloidu tiposomowego do zastosowań inhalacyjnych.
Influence of atomization method on the stability of liposomes being a novel carrier of inhalation medicines was studied. Measuring the droplet size and concentration of encapsulated tracer allowed one to demonstrate that pneumatic nebulization is destructive for liposomes due to high hydrodynamic stresses associated with liquid atomization. The VM nebulizer was shown to be less damaging for liposomes. The results allow for more rational selection of the atomizing device for delivery of atomized liposomes by inhalation.
Źródło:
Inżynieria i Aparatura Chemiczna; 2013, 5; 425--426
0368-0827
Pojawia się w:
Inżynieria i Aparatura Chemiczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of MLS laser radiation on cell lipid membrane
Autorzy:
Pasternak, K.
Wróbel, D.
Nowacka, O.
Pieszyński, I.
Bryszewska, M.
Kujawa, J.
Powiązania:
https://bibliotekanauki.pl/articles/2081559.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
liposomes
laser irradiation
laser therapy
MLS M1 system
Opis:
Introduction. Authors of numerous publications have proved the therapeutic effect of laser irradiation on biological material, but the mechanisms at cellular and subcellular level are not yet well understood. Objective. The aim of this study was to assess the effect of laser radiation emitted by the MLS M1 system (Multiwave Locked System) at two wavelengths (808 nm continuous and 905 nm pulsed) on the stability and fluidity of liposomes with a lipid composition similar to that of human erythrocyte membrane or made of phosphatidylocholine. Materials and method. Liposomes were exposed to low-energy laser radiation at surface densities 195 mW/cm2 (frequency 1,000 Hz) and 230 mW/cm2 (frequency 2,000 Hz). Different doses of radiation energy in the range 0–15 J were applied. The surface energy density was within the range 0.46 – 4.9 J/cm2. Results. The fluidity and stability of liposomes subjected to such irradiation changed depending on the parameters of radiation used. Conclusions. Since MLS M1 laser radiation, depending on the parameters used, affects fluidity and stability of liposomes with the lipid content similar to erythrocyte membrane, it may also cause structural and functional changes in cell membranes.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 1; 108-113
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Facilitated diffusion of glucosamine-6-phosphate synthase inhibitors enhances their antifungal activity.
Autorzy:
Janiak, Agnieszka
Cybulska, Barbara
Szlinder-Richter, Joanna
Borowski, Edward
Milewski, Sławomir
Powiązania:
https://bibliotekanauki.pl/articles/1043810.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
diffusion
liposomes
antifungal activity
synergism
GlcN-6-P synthase
Opis:
N3-(4-Methoxyfumaroyl)-L-2,3-diaminopropanoic acid (FMDP) and 2-amino-2-deoxy-D-glucitol-6-phosphate (ADGP) are strong inhibitors of the essential fungal enzyme, glucosamine-6-phosphate synthase, but their antifungal activity is poor, due to slow penetration of these agents through the cytoplasmic membrane. In the present studies we have exploited the possibility of enhancement of ADGP and FMDP antifungal activity by improving their transport properties. It has been found that membrane-permeabilising polyene macrolides amphotericin B (AMB) and its N-methyl-N-fructosyl methyl ester derivative (MF-AME), at subinhibitory concentrations, facilitate diffusion of ADGP through the fungal cell membrane, thus allowing a decrease of its minimal inhibitory concentration (MIC). Synergistic effects have been observed for combinations of ADGP with AMB or MF-AME. Fractional inhibitory concentration (FIC) indexes, determined against a number of Candida spp., have been in the 0.18-0.81 range. Weak antifungal synergistic effects have been found for combinations of FMDP with AMB or MF-AME. ADGP can be easily encapsulated into unilamellar lipid vesicles. Liposomal preparations of ADGP demonstrated stronger antifungal activity against some fungal strains than free ADGP.
Źródło:
Acta Biochimica Polonica; 2002, 49, 1; 77-86
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Peroxidation of proteins and lipids in suspensions of liposomes, in blood serum, and in mouse myeloma cells.
Autorzy:
Gebicki, Janusz
Du, Juan
Collins, James
Tweeddale, Helen
Powiązania:
https://bibliotekanauki.pl/articles/1044208.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
liposomes
lipid peroxides
mouse cells
serum
free radicals
protein peroxides
Opis:
There is growing evidence that proteins are early targets of reactive oxygen species, and that the altered proteins can in turn damage other biomolecules. In this study, we measured the effects of proteins on the oxidation of liposome phospholipid membranes, and the formation of protein hydroperoxides in serum and in cultured cells exposed to radiation-generated hydroxyl free radicals. Lysozyme, which did not affect liposome stability, gave 50% protection when present at 0.3 mg/ml, and virtually completely prevented lipid oxidation at 10 mg/ml. When human blood serum was irradiated, lipids were oxidized only after the destruction of ascorbate. In contrast, peroxidation of proteins proceeded immediately. Protein hydroperoxides were also generated without a lag period in hybrid mouse myeloma cells, while at the same time no lipid peroxides formed. These results are consistent with the theory that, under physiological conditions, lipid membranes are likely to be effectively protected from randomly-generated hydroxyl radicals by proteins, and that protein peroxyl radicals and hydroperoxides may constitute an important hazard to biological systems under oxidative stress.
Źródło:
Acta Biochimica Polonica; 2000, 47, 4; 901-911
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sinigrin Encapsulation in Liposomes: Influence on In Vitro Digestion and Antioxidant Potential
Autorzy:
Drvenica, Ivana
Blazević, Ivica
Bosković, Perica
Bratanić, Andre
Bugarski, Branko
Bilusić, Tea
Powiązania:
https://bibliotekanauki.pl/articles/2019142.pdf
Data publikacji:
2021-12-01
Wydawca:
Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
Tematy:
sinigrin
liposomes
oxidative stability
gastric digestion
duodenal digestion
antioxidant potential
Opis:
Encapsulation of sinigrin in liposomes with the proliposomal method was performed in order to evaluate the effect of this process on in vitro simulated digestion and antioxidant potential of sinigrin. The recovery of sinigrin after simulated gastric and duodenal digestion of its free and liposomal forms was determined with HPLC-UV using human digestive juices. The antioxidant potential of sinigrin and sinigrin-loaded liposomes was determined with the Rancimat test as their ability to prolong oxidative stability of edible oil . The efficiency of 62% was obtained by encapsulating sinigrin in liposomes. The values of mean diameter, polydispersity index and zeta potential showed satisfactory size uniformity and physical stability of the liposomes containing sinigrin. Liposomes were shown to inhibit the digestion of sinigrin in both human gastric and intestinal juices, clearly enabling its prolonged release. Moreover, sinigrin in the liposomal form significantly prolonged the induction time of edible oil oxidation compared to its free form. The results obtained are encouraging from the point of view of a possible incorporation of the sinigrin-loaded liposomes in real functional food systems or their use as nutraceuticals.
Źródło:
Polish Journal of Food and Nutrition Sciences; 2021, 71, 4; 441-449
1230-0322
2083-6007
Pojawia się w:
Polish Journal of Food and Nutrition Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Matrix metalloproteinase-3 induction following photodynamic therapy with liposomal formulations of aminolevulinic acid and its methyl ester
Indukcja metaloproteinazy-3 macierzy pozakomórkowej za pomocą terapii fotodynamicznej z zastosowaniem liposomowych preparatów kwasu aminolewulinowego i jego estru metylowego
Autorzy:
Jurczyszyn, K.
Czapińska, E.
Gamian, E.
Hotowy, K.
Terlecki, G.
Symonowicz, K.
Osiecka, B.
Bronowicz, A.
Ziółkowski, P.
Powiązania:
https://bibliotekanauki.pl/articles/261873.pdf
Data publikacji:
2010
Wydawca:
Politechnika Wrocławska. Wydział Podstawowych Problemów Techniki. Katedra Inżynierii Biomedycznej
Tematy:
terapia fotodynamiczna
liposomy
metvix
metaloproteinaza 3
photodynamic therapy
liposomes
metalloproteinase 3
Opis:
Photodynamic therapy (PDT) showed promising results in treatment of malignant and non-malignant disorders. The PDT requires for a therapeutic effect the combined action of photosensitizer and light. PDT causes direct cytotoxicity to malignant cells and may also have both direct and indirect effects upon various non-malignant components of tumor microenvironment. Unfortunately, some photosensitizers reveal low selectivity in pathologic tissues. Previous studies indicated that aminolevulinic acid (ALA) and its methyl ester (metvix) encapsulated in liposomes improved the quality and optimized results of PDT. Matrix metalloproteinases (MMPs) are enzymes implicated in various diseases by enabling the spread of disease. MMP-3 in turn, exerts the protective role in the development of tumors. We report the effect of liposomal formulation of ALA and metvix-based photodynamic therapy on induction of matrix metalloproteinase 3 in animal tumor model. Our results showed strong expression of MMP-3 in tumor-bearing rat tumor cells after PDT with liposomal formulations. The expression of MMP-3 in all tumor-bearing rats treated with PDT was stronger than those observed in animals free of tumors and especially in those treated with free precursor-PDT. The effect of liposomes was found to be important and these formulations elicited stronger intensity of immunohistochemical reactions than ALA- or metvix –PDT without liposomes.
Terapia fotodynamiczna (photodynamic therapy – PDT) daje obiecujące wyniki w leczeniu złośliwych i niezłośliwych chorób. PDT wymaga połączonego działania fotouczulacza i światła dla uzyskania efektu terapeutycznego. PDT wywołuje efekt cytotoksyczny w patologicznych komórkach. Może także działać bezpośrednio i pośrednio na różne składniki mikrośrodowiska nowotworu. Niestety, niektóre fotouczulacze mają niską selektywność w tkankach chorobowych. Poprzednie badania wykazały, że kwas aminolewulinowy (ALA) i jego ester metylowy (metvix) umieszczone w liposomach poprawiały jakość i wyniki PDT. Metaloproteinazy macierzy (MMPs) są enzymami wprzęgniętymi w różne choroby umożliwiającymi ich szerzenie się. Z kolei MMP-3 odgrywa rolę ochronną w rozwoju nowotworów. W pracy przedstawiono wpływ działania preparatów liposomowych ALA i metviksu w warunkach PDT na indukcję MMP-3 w zwierzęcym modelu nowotworowym. Uzyskane wyniki wskazują na silną ekspresję MMP- 3 w komórkach nowotworu po PDT z użyciem preparatów liposomowych. Ekspresja MMP-3 u wszystkich zwierząt zaszczepionych nowotworem i traktowanych PDT była silniejsza niż u zwierząt wolnych od nowotworu i szczególnie od tych leczonych PDT z użyciem wolnych prekursorów. Wpływ liposomów okazał się być istotny i przyczyniał się do silniejszej reakcji immunohistochemicznej niż PDT bez tych nośników.
Źródło:
Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna; 2010, 16, 2; 182-186
1234-5563
Pojawia się w:
Acta Bio-Optica et Informatica Medica. Inżynieria Biomedyczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nowe rozwiązania i modyfikacje najpopularniejszych polimerowych nośników substancji aktywnych
New solutions and modifications of the most popular polymeric carriers of active substances
Autorzy:
Odrobińska, Justyna
Neugebauer, Dorota
Powiązania:
https://bibliotekanauki.pl/articles/171574.pdf
Data publikacji:
2019
Wydawca:
Polskie Towarzystwo Chemiczne
Tematy:
koniugaty
micele
liposomy
nanocząstki
nośniki polimerowe
conjugates
micelles
liposomes
nanoparticles
polymeric carriers
Opis:
The progress of medicine and cosmetology corresponds to the increase in the need for new drug design and delivery methods, due to the already common cancer risk, as well as other diseases, which are still difficult for curing. Regardless of the type of active substance and its final application, the selection and synthesis of the appropriate carrier is crucial to provide the pharmaceutics to the target with the controlled release for a set period of time, including the intelligent activity. Depending on the way the bioactive substance is bound to carriers in the drug delivery systems (DDS) they are classified onto: carriers that physically encapsulate them inside i.e. liposomes, solid lipid nanoparticles, nanostructural lipid carriers, nanoparticles (nanocapsules, nanospheres), micelles, and carriers that chemically bind the active substance, i.e. conjugates, polyplexes. The current studies are emphasized to achieve the most perfect improvement, which concerns the efficiency of encapsulation, the ability to deliver several compounds simultaneously or sequentially, the productive release, the elimination of side products, the replacement of synthetic polymers by natural, biodegradable, biocompatible and non-toxic polymers. These carriers are also developed in terms of sensitivity to stimuli (one or several simultaneously or sequentially activated) and adapted for combined therapy. Although the subject of active substance delivery with the use of carriers is already widely studied, these are still a needs for the designing of new or the enhancement of the already known DDS. Working on this issue there is hope that today still incurable diseases, neoplastic diseases, as well as the diagnosis of these diseases themselves, will be possible to control and treat in the future. This report presents the current state of the knowledge in the area of DDS, focusing on newly developed solutions in the last years.
Źródło:
Wiadomości Chemiczne; 2019, 73, 9-10; 481-501
0043-5104
2300-0295
Pojawia się w:
Wiadomości Chemiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antitumoral effect of IL-12 gene transfected via liposomes into B16F0 cells
Autorzy:
Speroni, Lucía
Gasparri, Julieta
de los A. Bustuoabad, Victoria
Chiaramoni, Nadia
Smagur, Andrzej
Szala, Stanisław
Taira, María
del V. Alonso, Silvia
Powiązania:
https://bibliotekanauki.pl/articles/1040572.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
stearylamine
liposomes
B16F0 cells
B16 melanoma
IL-12
Opis:
Murine melanoma B16F0 cells were transfected with SA:DPPC:DOPE (2:1:1 molar ratio) liposomes associated with a plasmid encoding murine IL-12. Stearylamine, a cationic lipid, showed a greater transfection efficiency compared to DOTAP-containing liposomes. The lipid:DNA ratio was 2:1 (w/w). Control groups were mock transfected or transfected with an empty plasmid (pNeo). pNeo or IL-12 transfected cells and controls were inoculated intradermically into the dorsal region of the foot or the lateral flank of C57BL6 mice. Results showed that IL-12 expression had a marked effect on in vivo growth of B16 melanoma tumors developed in both anatomic sites, significantly retarding their growth and prolonging host survival.
Źródło:
Acta Biochimica Polonica; 2009, 56, 2; 249-253
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
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