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    Wyświetlanie 1-4 z 4
    Tytuł:
    Bakteriofagi – nanocząstki o szerokich zastosowaniach
    Bacteriophages – nanoparticles of wide application range
    Autorzy:
    Szermer-Olearnik, B.
    Boratyński, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/141815.pdf
    Data publikacji:
    2014
    Wydawca:
    Stowarzyszenie Inżynierów i Techników Przemysłu Chemicznego. Zakład Wydawniczy CHEMPRESS-SITPChem
    Tematy:
    bakteriofagi
    endotoksyny
    lipopolisacharydy
    oczyszczanie
    bacteriophages
    endotoxins
    lipopolysaccharides
    purification
    Opis:
    Bakteriofagi są wirusami infekującymi wybiórczo komórki bakteryjne. Zainfekowana bakteria może ulec lizie uwalniając wiriony potomne, wraz z którymi uwalniane są do środowiska fragmenty ściany komórkowej bakterii np. lipopolisacharydy (endotoksyny; pirogeny). Istotnym aspektem przygotowania preparatów bakteriofagowych jest standaryzacja procedur ich wytwarzania i przechowywania, między innymi spełnienie szeregu wymagań dotyczących apirogenności. Opracowanie przedstawia historię odkrycia bakteriofagów, potencjalne zastosowania terapeutyczne oraz metody oczyszczania preparatów.
    Bacteriophages are viruses that infect selectively bacterial cells. The infected bacterium may undergo bacteriolysis releasing descendant virions, along with which fragments of bacterium cell wall, e.g. lipopolysaccharides (endotoxins; pyrogens) are also released. The important aspect of preparing bacteriophage preparations is standardisation of production and storage procedures, i.a. satisfying number of requirements related to their apyrogenicity. This work presents history of bacteriophage discovery, their potential therapeutic applications and methods for purification of preparations.
    Źródło:
    Chemik; 2014, 68, 9; 761-765
    0009-2886
    Pojawia się w:
    Chemik
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Chemical characterization of effective and ineffective strains of Rhizobium leguminosarum bv. viciae
    Autorzy:
    Izmailov, Stanislav
    Zhiznevskaya, Genrietta
    Kosenko, Larisa
    Troitskaya, Galina
    Kudryavtseva, Nataly
    Borodenko, Ludmila
    Dubrovo, Pavel
    Russa, Ryszard
    Pietras, Hubert
    Lorkiewicz, Zbigniew
    Powiązania:
    https://bibliotekanauki.pl/articles/1044461.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    lipopolysaccharides
    fatty acids
    Rhizobium leguminosarum bv. viciae
    Opis:
    Chemical composition of lipopolysaccharide (LPS) isolated from an effective (97) and ineffective (87) strains of R. l. viciae has been determined. LPS preparations from the two strains contained: glucose, galactose, mannose, fucose, arabinose, heptose, glucosamine, galactosamine, quinovosamine, and 3-N-methyl-3,6-dideoxyhexose, as well as glucuronic, galacturonic and 3-deoxyoctulosonic acid. The following fatty acids were identified: 3-OH 14:0, 3-OH 15:0, 3-OH 16:0, 3-OH 18:0 and 27-OH 28:0. The ratio of 3-OH 14:0 to other major fatty acids  in LPS 87 was higher that in LPS 97. SDS/PAGE profiles of LPS indicated that, in lipopolysaccharides, relative content of S form LPS I to that of lower molecular mass (LPS II) was much higher in the effective strain 97 than in 87. All types of polysaccharides exo-, capsular-, lipo, (EPS, CPS, LPS, respectively) examined possessed the ability to bind faba bean lectin. The degree of affinity of the host lectin to LPS 87 was half that to LPS 97. Fatty acids (FA) composition from bacteroids and peribacteroid membrane (PBM) was determined. Palmitic, stearic and hexadecenoic acids were common components found in both strains. There was a high content of unsaturated fatty acids in bacteroids as well as in PBM lipids. The unsaturation index in the PBM formed by strain 87 was lower than in the case of strain 97. Higher ratio of 16:0 to 18:1 fatty acids was characteristic for PMB of the ineffective strain.
    Źródło:
    Acta Biochimica Polonica; 1999, 46, 4; 1001-1009
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    ANTI-INFLAMMATORY ACTIVITY AND CYTOTOXICITY OF DIOSGENIN ON LIPOPOLYSACCHARIDES INDUCED RAW 264.7 CELLS
    Autorzy:
    ning, jing
    chang, yu
    wang, ruxia
    lan, zhiwei
    ru, qing
    liu, mingchun
    tian, chunlian
    Powiązania:
    https://bibliotekanauki.pl/articles/895615.pdf
    Data publikacji:
    2020-04-29
    Wydawca:
    Polskie Towarzystwo Farmaceutyczne
    Tematy:
    Diosgenin
    Anti-inflammatory
    lipopolysaccharides
    cytotoxicity
    phagocytosis ability  
    Opis:
    Diosgenin is a steroidal sapogenin compound, and possesses multiple biological activities including anti-inflammatory, anticancer, immunological regulation, and anti-aging. The current study focused on its anti-inflammatory activities and cytotoxicity by analysis of NO production, phagocytosis activity, secretion of TNF-α and IL-6 and cell viability in LPS-induced RAW 264.7 cells. An IC50 value of diosgenin of 2.8 μM was calculated for diosgenin by regression of cell viability from concentrations ranging from 0.01 to 25 μM; this indicated that 0.01, 0.02, 0.04 μM diosgenin could reduce phagocytic activity very significantly (p<0.01) in a dose-dependent manner with no cytotoxic effect on the LPS-induced RAW 264.7 cells. However, there was no significant effect on NO content and secretion of TNF-α and IL-6 after diosgenin treatment. The research revealed that low concentrations diosgenin can directly inhibit cells phagocytosis, with no effect on the release of inflammatory mediators and cytokines. This lays a foundation for screening for a safe dose in research and developent of derivatives and new formulation of diosgenin for its anti-inflammatory effect.
    Źródło:
    Acta Poloniae Pharmaceutica - Drug Research; 2020, 77, 2; 313-317
    0001-6837
    2353-5288
    Pojawia się w:
    Acta Poloniae Pharmaceutica - Drug Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Lipopolysaccharides from Mesorhizobium huakuii and Mesorhizobium ciceri: chemical and immunological comparative data.
    Autorzy:
    Choma, Adam
    Powiązania:
    https://bibliotekanauki.pl/articles/1043713.pdf
    Data publikacji:
    2002
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    immunobloting
    lipopolysaccharides
    2,3-diamino-2,3-dideoxy-D-glucose
    fatty acids
    Mesorhizobium huakuii
    Mesorhizobium ciceri
    sugars
    Opis:
    Lipopolysaccharides of two Mesorhizobium species of different host specificity were compared: M. huakuii and M. ciceri. M. huakuii sp. was represented by five strains with special consideration of M. huakuii IFO 15243T. SDS/PAGE profiles revealed that all M. huakuii LPS preparations contained low molecular mass fractions (LPS-II) of the same molecular size. All of lipopolysaccharides contained high molecular mass fractions (LPS-I). However, the high molecular mass fraction from each strain possessed an individual molecular size distribution pattern. The crossreactivity of blotted lipopolysaccharides with rabbit polyclonal antibodies against Mesorhizobium huakuii IFO 15243T whole bacteria indicated the presence of common epitope(s) within the investigated Mesorhizobium huakuii strains. Moreover, LPS from M. huakuii S52 also reacted with anti M. ciceri HAMBI 1750 serum showing that there are epitopes common for different mesorhizobial species. LPS isolated from Mesorhizobium huakuii strain IFO 15243T contained neutral sugars: L-6-deoxytalose, L-rhamnose, D-galactose and D-glucose, aminosugars: D-quinovosamine, D-glucosamine, D-2,3-diamino-2,3-dideoxyglucose and D-galacturonic and D-glucuronic acids. In the LPS preparation, fatty acids typical for Mesorhizobium strains were detected. 3-Hydroxydodecanoic, 3-hydroxy-iso-tridecanoic, 3-hydroxyeicosanoic, 3-hydroxyheneicosanoic and 3-hydroxydocosenoic acids were the major amide linked fatty acids, while iso-heptadecanoic, eicosanoic, docosenoic, as well as 27-hydroxyoctacosanoic and 27-oxooctacosanoic acids were the dominant ester linked fatty residues.
    Źródło:
    Acta Biochimica Polonica; 2002, 49, 4; 1043-1052
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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