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    Tytuł:
    The number of E. coli and C. perfringens bacteria in poultry waste and subsequent phases of composting
    Liczebność bakterii E. coli i C. perfringens w odpadach drobiarskich oraz kolejnych fazach ich kompostowania
    Autorzy:
    Cybulska, K.
    Suchecka, N.
    Wrońska, I.
    Mahdi-Oraibi, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/125776.pdf
    Data publikacji:
    2015
    Wydawca:
    Towarzystwo Chemii i Inżynierii Ekologicznej
    Tematy:
    E.coli
    C. perfringens
    poultry waste
    composting
    E. coli
    odpady drobiarskie
    kompostowanie
    Opis:
    Rapidly growing commercial poultry production generates large amounts of waste. Waste that accumulates during the poultry slaughter process often remains unprocessed, becoming a serious threat to people’s health and the natural environment. Poultry production waste constitutes problems odour threat and dangerous sanitary threat. The aim of this study was to determine the population size of Escherichia coli and Clostridium perfringens in poultry waste and in successive stages of waste composting. Research material consisted of raw feathers collected immediately after the slaughter, samples of biological sludge from the centrifuge, mixture of straw, feathers and lime and processed compost. Microbiological analyses were conducted with the use of spread plate count method and the substrate was used in accordance with research standards. The size of population of microorganisms in the samples analyzed corresponded to the waste processing stage and group of microbes. The presence of C. perfringens strains was ascertained in all samples, whereas E. coli strains were identified only in raw feathers and centrifuge sludge, being most numerous in unprocessed material. A reverse trend was observed in case of C. perfringens with the highest population density in centrifuge sludge and least density in raw feathers.
    Intensywnie rozwijająca się produkcja drobiarska wiąże się z generowaniem dużej ilości odpadów. Powstające produkty uboczne podczas uboju pozostawione w stanie surowym stają się poważnym zagrożeniem dla środowiska naturalnego i zdrowia ludzi. Mogą stwarzać problemy odorotwórcze oraz istotnie groźne zanieczyszczenie sanitarne. Celem przeprowadzonych badań było określenie liczebności E. coli i C. perfringens w odpadach drobiarskich oraz kolejnych etapach ich kompostowania. Materiał badawczy stanowiły świeże pióra bezpośrednio po uboju, osad biologiczny z wirówki, mieszanina słomy, pierza i wapna oraz kompost przerobiony. Analizy mikrobiologiczne wykonano metodą płytkową, wykorzystując wybiórcze podłoża zgodne z normami. Liczebność mikroorganizmów w badanych próbach zależała od fazy przerobu odpadu i analizowanej grupy drobnoustrojów. Obecność bakterii C. perfringens stwierdzono we wszystkich próbach, natomiast bakterie E. coli tylko w piórach surowych i osadzie z wirówki, przy czym najliczniej występowały w materiale nieprzetworzonym. Odwrotną tendencję zaobserwowano w przypadku C. perfringens, które najliczniej zasiedlały osad z wirówki, natomiast ich mniejszą liczebność stwierdzono w piórach surowych.
    Źródło:
    Proceedings of ECOpole; 2015, 9, 1; 19-24
    1898-617X
    2084-4557
    Pojawia się w:
    Proceedings of ECOpole
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sanitary evaluation of the water from water intake points situated in Gorzow Wielkopolski and neighbouring localities
    Ocena sanitarna wody pochodzącej z ujść wodnych na terenie Gorzowa Wielkopolskiego oraz okolic
    Autorzy:
    Wrońska, I.
    Cybulska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/388391.pdf
    Data publikacji:
    2015
    Wydawca:
    Towarzystwo Chemii i Inżynierii Ekologicznej
    Tematy:
    drinking water
    bacteria
    E.coli
    E. faecalis
    water contamination
    woda pitna
    bakteria
    E. coli
    zanieczyszczenie wody
    Opis:
    Water is an essential component necessary for sustaining life. Nevertheless, it may pose a serious threat to human life resulting from possible occurrence of pathogenic and potentially pathogenic microorganisms. Therefore, monitoring and continuous microbial surveillance of the water intended for consumption is extremely important. The requirements referring to qualitative parameters which should be met by water are set out in the Regulation of the Minister of Health of 29 March 2007, as amended on 20 April 2010, and the EU directives. The quality of water being supplied to consumers depends on many factors, among other on the type of water intake (surface water or groundwater), efficiency of water treatment and disinfection, and technical condition of the water supply network. The analysis of the sanitary condition of drinking water has been conducted form water intakes in the city of Gorzow Wielkopolski, ie Centralny Kosynierow Gdynskich intake, Klodawa intake and Siedlice intake, and in neighbouring localities, ie. Maszewo and Marwice. The research material was raw and treated water samples. The population size of Faecal streptococci, coliform bacteria, including E. coli, and total mesophilic and psychrophilic bacteria counts was determined. The analysis of the sanitary condition of drinking water has been conducted. The number of coliform bacteria, including E. coli, and streptococci was determined by the method of membrane filters, whereas total mesophilic and psychrophilic bacteria counts using the plate method under incubation conditions conforming to the standards. The presence of mesophilic and psychrophilic bacteria was observed in all examined water intake points. Differences in their counts over the whole year being significant. In three research points, ie Centralny Kosynierow Gdynskich intake, Maszewo intake and Marwice intake, psychrophilic bacteria count was exceeded (100 CFU ۰ cm–3). In the case of pathogenic bacteria, their occurrence was occasionally observed in the drinking water intake points. The water treatment processes applied and, as the final result, water sanitary assessment according to the existing standards and the provision stipulated allow it to be collected for consumption purposes and do not pose the threat to human health and life.
    Woda jest podstawowym składnikiem niezbędnym do utrzymania życia. Jednakże, może ona stworzyć poważne zagrożenie dla zdrowia człowieka wynikające z możliwości wystąpienia mikroorganizmów chorobotwórczych i potencjalnie chorobotwórczych. W związku z tym, niezwykle istotna jest kontrola oraz stały nadzór mikrobiologiczny nad wodą przeznaczoną do spożycia. Wytyczne co do parametrów jakościowych, jakim powinna odpowiadać, zawarte są w Rozporządzeniu Ministra Zdrowia z dnia 29 marca 2007 r. wraz ze zmianą z dnia 20 kwietnia 2010 r. oraz Dyrektywach Unii Europejskiej. Jakość wody dostarczanej konsumentom zależy od wielu czynników m.in.: rodzaju ujęcia (woda powierzchniowa lub podziemna), skuteczności procesów uzdatniania i dezynfekcji oraz stanu technicznego sieci wodociągowej. Przeprowadzono analizę stanu sanitarnego wody pitnej pochodzącej z ujęć wodnych zaopatrujących miasto Gorzów Wielkopolski, tj. ujęcie Centralne Kosynierów Gdyńskich, Kłodawa, Siedlice oraz sąsiadujące miejscowości, tj. Maszewo i Marwice. Materiałem badawczym były próbki wody surowej i uzdatnionej. Określono liczebność paciorkowców kałowych, bakterii z grupy coli, w tym E. coli oraz ogólnej liczebności bakterii mezofilnych i psychrofilnych. Liczebność bakterii z grupy coli, w tym E. coli oraz paciorkowców kałowych oznaczono metodą filtrów membranowych, zaś ogólną liczebność bakterii mezofilnych i psychrofilnych metodą płytkową w warunkach inkubacji zgodnych z normami. We wszystkich badanych punktach stwierdzono obecność bakterii mezofilnych i psychrofilnych. Różnice w ich liczebności na przestrzeni całego roku były istotne. W punktach badawczych, tj. Centralne Kosynierów Gdyńskich, Maszewo oraz Marwice odnotowano przekroczenie norm ilości psychrofilii 100 j.t.k. ۰ cm–3. Obecność bakterii chorobotwórczych w badanych próbkach wody pitnej wykryto sporadycznie. Zastosowane procesy uzdatniania i w efekcie końcowym ocena sanitarna wody według obowiązujących norm i zawartej klauzuli pozwalają na jej pobór do spożycia i nie stanowią zagrożenia dla zdrowia i życia ludzi.
    Źródło:
    Ecological Chemistry and Engineering. A; 2015, 22, 1; 17-26
    1898-6188
    2084-4530
    Pojawia się w:
    Ecological Chemistry and Engineering. A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Escherichia coli in sewage sludge - detection method
    Escherichia coli w osadach ściekowych - metoda wykrywania
    Autorzy:
    Machnicka, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/106338.pdf
    Data publikacji:
    2014
    Wydawca:
    Towarzystwo Chemii i Inżynierii Ekologicznej
    Tematy:
    E.coli
    sewage sludge
    enrichment of Escherichia coli
    detection of Escherichia coli
    E. coli
    osady ściekowe
    namnażanie Escherichia coli
    wykrywanie Escherichia coli
    Opis:
    Escherichia coli is Gram-negative optionally anaerobic roads which belongs to Enterobacteriaceae family. Includes in a physiological bacterial flora of human and warm-blooded animals large intestine. Escherichia coli is being met in abiotic elements of the environment so as waters, wastewater, sewage sludge, soil and the food. This bacterium is showing the pathogenicity in named terms for the peoples, triggering diseases mainly: gastrointestinal tract and urinary. Quality and quantitative proposed detections method of the bacteria E. coli contains five/six steps: - appointment dry suspended solid, - preparation averaged, test of sample and resuscitation of bacteria, - making dilutions, - enrichment and differentiation in chromogenic-selective medium, - enumerating the amount of cfu E. coli in 1 g of a dry weight, - optionally, the biochemical identification.
    Escherichia coli jest Gram-negatywną, względnie beztlenową pałeczką należącą do rodziny Enterobacteriaceae. Wchodzi w skład fizjologicznej flory bakteryjnej jelita grubego człowieka oraz zwierząt stałocieplnych. Spotyka się ją w elementach abiotycznych środowiska, takich jak wody, ścieki, osady ściekowe, gleba i żywność. Bakteria ta w określonych warunkach wykazuje chorobotwórczość dla człowieka, wywołując głównie schorzenia układów pokarmowego i moczowego. Proponowana metoda jakościowa i ilościowa wykrywania bakterii E. coli zawiera pięć/sześć etapów: - oznaczenie suchej masy osadu, - przygotowanie próbki uśrednionej i badawczej oraz przywrócenie bakteriom aktywności fizjologicznej, - wykonanie rozcieńczeń, - namnażanie i identyfikacja na podłożu chromogennym-selektywnym, - wyliczenie ilości jtk E. coli w 1 g suchej masy osadu, - opcjonalnie identyfikacja biochemiczna.
    Źródło:
    Chemistry-Didactics-Ecology-Metrology; 2014, 19, 1-2; 79-85
    2084-4506
    Pojawia się w:
    Chemistry-Didactics-Ecology-Metrology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Maillard neoglycans as inhibitors for in vitro adhesion of F4+ enterotoxigenic Escherichia coli to piglet intestinal cells
    Autorzy:
    Sarabia-Sainz, Héctor
    Mata Haro, Verónica
    Sarabia Sainz, José
    Vázquez-Moreno, Luz
    Montfort, Gabriela
    Powiązania:
    https://bibliotekanauki.pl/articles/1038559.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Albumin glycation
    biorecognition
    anti-adhesion
    E. coli
    Opis:
    Adhesion of enterotoxigenic (ETEC) E. coli to host intestinal cells is mediated by lectin-like fimbriae that bind to specific glycan moieties on the surfaces of enterocytes. To prevent in vitro binding of E. coli F4 fimbriae (F4 ETEC+) to piglet enterocytes, neoglycans were synthesized by the Maillard reaction conjugating lactose (Lac), galacto-oligosaccharides (GOS) or chitin oligosaccharides (Ochit) to porcine serum albumin (PSA). Neoglycans were characterized by SDS-PAGE, intrinsic tryptophan fluorescence and recognition by plant lectins, as well as by F4 ETEC variants. Electrophoretic patterns suggested the binding to PSA of 63, 13 and 2 molecules of Lac, GOS and Ochit, respectively. All neoglycans displayed quenching of tryptophan fluorescence consistent with the degree of glycation estimated by SDS-PAGE. Plant lectins recognized the neoglycans according to their specificity, whereas antigenic variants of F4 ETEC (ab, ac and ad) recognized PSA-Ochit and PSA-Lac with higher affinity than that for GOS. Neoglycans partially hindered the in vitro binding of F4+ ETEC to piglet enterocytes in a dose-dependent manner. The most effective blocking was observed with PSA-Lac that partially inhibited the adhesion of bacteria to enterocytes in a dose dependent manner, as quantified by flow cytometry. Increased production of the cytokines IL-6 and TNF-α was observed in response to F4+ ETEC infection of enterocytes and production was reduced in the presence of PSA-Ochit and PSA-GOS. These results suggest that neoglycans synthesized by the Maillard reaction could be useful in the prophylaxis of diarrhea in piglets.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 4; 679-686
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microbiological air rating in a variety of objects during treatment of the post-slaughter poultry wastes Part I. Escherichia coli
    Autorzy:
    Oraibi, S. M.
    Cybulska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/125448.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Inżynierii Ekologicznej
    Tematy:
    E.coli
    air
    poultry waste facilities
    composting
    Opis:
    The presence of Escherichia coli in the air of facilities involved in management and composting of post-slaughter poultry wastes in selected plants of West Western Pomerania region was studied. Measurements were made on four dates in a variety of weather conditions during the year. The study was conducted at 5 objects that differ in the type of waste and the degree of preparation for composting. These were: chemical treatment and preliminary processing plant, liquid wastes reservoir, platform for preparation of materials for composting, storage of biological sediments, and composting facility. Measurement of bacteria count was carried out in accordance with the applicable procedures on selective chromogenic TBX medium. The assays revealed the presence of E. coli at all test objects, but not always on all measurement dates. It has been shown that the presence of E. coli was from 20 to 3047 CFUm-3 of air, although the largest quantities were most frequently detected in the air of the building for post-slaughter waste pre-treatment in chemical treatment plant.
    Źródło:
    Journal of Ecological Engineering; 2016, 17, 5; 17-21
    2299-8993
    Pojawia się w:
    Journal of Ecological Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Type 1 fimbriae in commensal Escherichia coli derived from healthy humans
    Autorzy:
    Pusz, Paweł
    Bok, Ewa
    Mazurek, Justyna
    Stosik, Michał
    Baldy-Chudzik, Katarzyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1039310.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    commensal E. coli
    type 1 fimbriae
    gene expression
    Opis:
    Type 1 fimbriae are one of the most important factors of Escherichia coli adaptation to different niches in the host. Our study indicated that the genetic marker - fimH gene occurred commonly in commensal E. coli derived from healthy humans but expression of the type 1 fimbriae was not observed. Identification of fim structural subunit genes (fimA-fimH) and recombinase fimE and fimB genes showed that many of the strains were carrying an incomplete set of genes and the genes expression study revealed that in strains with complete set of fim genes, the fimC gene, encoding the chaperone protein, was not expressed.
    Źródło:
    Acta Biochimica Polonica; 2014, 61, 2; 389-392
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Isolation of bacteria from soil sample of Tamil Nadu and their in vitro interaction
    Autorzy:
    Sankari, D.
    Khusro, Ameer
    Aarti, Chirom
    Powiązania:
    https://bibliotekanauki.pl/articles/1031683.pdf
    Data publikacji:
    2020
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Antagonism
    B. subtilis
    CFU
    E. coli
    Interaction
    P. aeruginosa
    Opis:
    The present study was investigated to determine the competitive interaction between Gram positive (B. subtilis) and Gram negative (P. aeruginosa and E. coli) bacteria in vitro. Bacteria of interest were isolated from soil sample and were identified through morphological and biochemical tests. Bacterial cultures were grown alone and as co- culture in test tubes containing sterile broth. Bacterial growth and their growth inhibition in co- culture tubes were analyzed till 96 h through spectrophotometric assay and colony forming unit (CFU) assay. Spectrophotometric analysis and CFU/mL assay showed that the effect of B. subtilis on P. aeruginosa and E. coli in co- culture tubes was growth inhibitory. This is accompanied by the reduction in absorbance value and CFU/mL of the co-culture tubes. The bactericidal activity of B. subtilis was measured on P. aeruginosa and E. coli using agar well diffusion method. B. subtilis showed zone of inhibition of 8 mm and 6 mm against P. aeruginosa and E. coli, respectively. The antagonistic activity test of B. subtilis favoured the spectrophotometric and colony forming assay results. These results suggest that B. subtilis develop adaptational pathways by extracellular signaling molecules and antibacterial factors in order to compete with bacterial neighbors.
    Źródło:
    World News of Natural Sciences; 2020, 28; 67-75
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    CEA-negative glioblastoma and melanoma cells are sensitive to cytosine deaminase/5-fluorocytosine therapy directed by the carcinoembryonic antigen promoter.
    Autorzy:
    Dąbrowska, Anna
    Szary, Jarosław
    Kowalczuk, Małgorzata
    Szala, Stanisław
    Ugorski, Maciej
    Powiązania:
    https://bibliotekanauki.pl/articles/1041550.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    CEA promoter
    E. coli cytosine deaminase
    gene-directed enzyme prodrug therapy
    Opis:
    Recent studies have suggested that carcinoembryonic antigen (CEA)-promoter sequences are active only in CEA-positive cells, filing in the criteria for tumor specific targeting of suicide genes. However, the present study on gene therapy of colon cancer and cell-specificity of CEA promoter, provide evidence that CEA-positive and CEA-negative cells transfected with E. coli cytosine deaminase (CD) gene under the control of CEA promotor sequence are sensitive to enzyme/pro-drug therapy with 5-fluorocytosine (5-FC). Individual clones derived from the CEA-negative cell lines: melanoma Hs294T and glioblastoma T98G after transfection with CD differed profoundly in their sensitivity to 5-FC. The IC50 values for several clones of the CEA-negative cells were almost the same as for CEA-positive colon cancer cells. Such 5-FC-sensitive clones derived from the population of CEA-negative cells, present even in small number, because of the very effective bystender effect of this enzyme/pro-drug system can cause severe problems during therapy by efficiently killing surrounding normal cells. Safety is the major issue in gene therapy. Our data suggest that the safety of gene-directed enzyme pro-drug therapy (GDEPT) with CEA promoter driven expression of therapeutic genes is not so obvious as it has originally been claimed.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 3; 723-732
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection and enumeration of Enteric bacteria associated with food handlers and surfaces of food manufacturing industry located in Hub city, Pakistan
    Autorzy:
    Ali, Razim
    Hayat, Amir
    Fatima, Madiha
    Noman, Muhammad
    Powiązania:
    https://bibliotekanauki.pl/articles/1182928.pdf
    Data publikacji:
    2016
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    enterobacteriaceae
    foodborne microbes
    hygiene
    surface contamination
    food handlers
    e. coli
    sanitation
    Opis:
    Foodborne microorganisms harbor and adheres itself to the food material and surrounding surfaces for a long time and influence the food quality and consumers health. Among these microbes the presence of Enteric indicator bacteria in food premises confers the indication of entero-pathogens, i.e. E. coli, Salmonella, Shigella and Campylobacters that could cause severe systemic infections in consumers. In this study, the hygienic status of confectionery and supplementary food processing facility was evaluated. A total of 10497 examinations were performed on 3499 swab samples collected from food premises and handlers for the analysis of Enteric indicator bacteria. From swabs, 1277 (12.2%) isolates were identified in which Enterobacteriaceae were found with higher frequency 604 (47.3%) followed by Coliforms 293 (30.8%) and Escherichia coli 280 (21.9%) respectively. The mean count (CFU/cm2) was found maximum for plain surfaces (floors, walls and door), while the lowest was for equipment and machinery. Overall isolates percent prevalence was determined where Enterobacteriaceae were 47%, Coliforms 31% and Escherichia coli 22%. Majority of the floor surfaces were highly contaminated, where washing and sanitation practices were observed to be inappropriate. Worker hygiene status was lacking essential food safety and hygiene standards. In general, the Enteric bacteria were found with higher ratio, that could affect the food quality and quantity both to a greater extent with some influences on consumers health.
    Źródło:
    World Scientific News; 2016, 49, 2; 192-203
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mutator specificity of Escherichia coli alkB117 allele
    Autorzy:
    Nieminuszczy, Jadwiga
    Janion, Celina
    Grzesiuk, Elżbieta
    Powiązania:
    https://bibliotekanauki.pl/articles/1041261.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    lacZ → Lac+ reversion
    mutational specificity
    E. coli
    MMS
    alkB117
    Opis:
    The Escherichia coli AlkB protein encoded by alkB gene was recently found to repair cytotoxic DNA lesions 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) by using a novel iron-catalysed oxidative demethylation mechanism that protects the cell from the toxic effects of methylating agents. Mutation in alkB results in increased sensitivity to MMS and elevated level of MMS-induced mutations. The aim of this study was to analyse the mutational specificity of alkB117 in a system developed by J.H. Miller involving two sets of E. coli lacZ mutants, CC101-106 allowing the identification of base pair substitutions, and CC107-CC111 indicating frameshift mutations. Of the six possible base substitutions, the presence of alkB117 allele led to an increased level of GC→AT transitions and GC→TA and AT→TA transversions. After MMS treatment the level of GC→AT transitions increased the most, 22-fold. Among frameshift mutations, the most numerous were -2CG, -1G, and -1A deletions and +1G insertion. MMS treatment appreciably increased all of the above types of frameshifts, with additional appearance of the +1A insertion.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 2; 425-428
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    COMPARATIVE IN VITRO STUDIES OF FURAZIDIN AND NITROFURANTOIN ACTIVITIES AGAINST COMMON UROPATHOGENS INCLUDING MULTIDRUG-RESISTANT STRAINS OF E. COLI AND S. AUREUS
    Autorzy:
    Klesiewicz, Karolina
    Karczewska, Elżbieta
    Nowak, Paweł
    Mrowiec, Paulina M.
    Skiba-Kurek, Iwona
    Białecka, Joanna
    Majka, Zbigniew
    Berdzik-Kalarus, Sylwia
    Budak, Alicja
    Zajdel, Paweł
    Powiązania:
    https://bibliotekanauki.pl/articles/895689.pdf
    Data publikacji:
    2018-06-30
    Wydawca:
    Polskie Towarzystwo Farmaceutyczne
    Tematy:
    ESBL-positive E. coli
    MRSA
    furazidin
    nitrofurantoin
    urinary tract infections
    Opis:
    Abstract: Urinary tract infections caused by wide range of pathogens including gram-negative and gram-positive bacteria as well as fungi are a severe public health problem. The predominant causative agent of both uncomplicated and complicated urinary tract infections is Escherichia coli. In an era of increasing bacterial resistance to antimicrobial agents and a high prevalence of multidrug-resistant (MDR) strains in community and hospital acquired infections, the re-evaluation of older generations of antimicrobial agents, such as nitrofuran derivatives, seems to be a reasonable approach. The aim of the study was to evaluate furazidin activity against common uropathogens in comparison to nitrofurantoin and other selected antimicrobial agents, routinely used in the treatment of urinary tract infections. Furazidin exhibited lower MICs than nitrofurantoin when tested against gram-negative and gram-positive bacteria including clinical MDR E. coli and methicillin-resistant Staphylococcus aureus. The MICs for furazidin ranged from 4 to 64 mg/L for Enterobacteriaceae strains, from 2 to 4 mg/L for gram-positive cocci, and 0.5 mg/L for anaerobic bacteria. The MICs for nitrofurantoin ranged from 16 to 64 mg/L for Enterobacteriaceae strains, from 8 to 64 mg/L for gram-positive cocci, and 4 mg/L for anaerobic bacteria. In addition, both nitrofurans displayed better activity against the tested bacterial strains than ciprofloxacin, fosfomycin, trimethoprim and co-trimoxazole. Nitrofuran derivatives displayed higher antimicrobial activity than other antimicrobial agents regardless of bacteria species or resistance mechanism.
    Źródło:
    Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 3
    0001-6837
    2353-5288
    Pojawia się w:
    Acta Poloniae Pharmaceutica - Drug Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Design of small molecule inhibitors of type III secretion system ATPase EscN from enteropathogenic Escherichia coli
    Autorzy:
    Bzdzion, Lukasz
    Krezel, Hanna
    Wrzeszcz, Karol
    Grzegorek, Irmina
    Nowinska, Katarzyna
    Chodaczek, Grzegorz
    Swietnicki, Wieslaw
    Powiązania:
    https://bibliotekanauki.pl/articles/1038684.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Escherichia coli (E. coli)
    type III secretion system (T3SS)
    enzyme inhibitor
    small molecule
    Opis:
    Enteropathogenic E. coli (EPEC) is a human pathogen using type III secretion system for delivery of proteins directly into the human host. The system contains a single ATPase, EscN, which is essential for uncoupling of proteins from their complexes with chaperones before the delivery. The structure of EscN ATPase (PDB code: 2obm) was used to screen computationally for small molecule inhibitors blocking its active site. Two lead candidates were examined but only one, Compound 54, was selected for further optimization. After extended QSAR optimization, two derivatives were found to be competitive inhibitors of EscN capable of blocking ATPase activity with a Ki below 50 µM. One candidate, WEN05-03, with a Ki=16±2 µM, was also minimally toxic to mammalian cells as determined by other assays. In the cell infection model of HeLa cells with EPEC, Compound WEN05-03 completely blocked actin cluster formation at 100 µM concentration, when analyzed by confocal microscopy. The second best inhibitor of EscN ATPase activity was WEN04-34 with a Ki=46±2 µM. However, the compound was highly toxic to the BALB/3T3 cell line. In summary, the work identifies a compound blocking bacterial ATPase in its active site without causing cellular toxicity to the host cells. It is the first report showing feasibility of using bacterial virulence system ATPase as a target for safe, non-toxic compounds and offering a proof-of-concept for non-antibiotic alternatives.
    Źródło:
    Acta Biochimica Polonica; 2017, 64, 1; 49-63
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Assessment of microbiological quality of water in the Nowohucki Reservoir with particular regard to microorganisms potentially dangerous to humans
    Autorzy:
    Wolny-Koładka, Katarzyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1035482.pdf
    Data publikacji:
    2016
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    "E. coli"
    "E. faecalis"
    "Nowohucki Reservoir"
    "mesophilic bacteria"
    "microbiological contamination of water"
    "surface water"
    Opis:
    Introduction. This study was aimed to assess the microbiological quality of water in the Nowohucki Reservoir (Kraków, Poland) as well as to determine whether its waters contain microorganisms potentially dangerous from an epidemiological point of view. Material and methods. Microbiological analyses included the determination of the number of mesophilic and psychrophilic bacteria, coliforms, fecal E. coli, as well as E. faecalis, C. perfringens, Staphylococcus spp. and Salmonella spp.. Water samples were collected 4 times per year on April 27th 2015 (spring), July 10th 2015 (summer), October 12th 2015 (autumn) and December 29th 2015 (winter) at 5 points within the area of the reservoir. Water and air temperature was measured onsite. Results. It was found that the prevalence of the analyzed microorganisms was affected by changing water and air temperature as well as by using this reservoir during holiday season for swimming purposes by local residents. All analyzed microbiological indicators of poor water quality were found in the analyzed water samples, which may pose a potential health risk to people swimming in the considered reservoir. Conclusions. From an epidemiological point of view, it is reasonable to include the Nowohucki Reservoir into a constant sanitary monitoring programme.
    Wstęp. Badania miały na celu ocenę stanu mikrobiologicznego wód Zalewu w Nowej Hucie (Kraków, Polska) a także określenie, czy w zbiorniku występują drobnoustroje potencjalnie niebezpieczne z epidemiologicznego punktu widzenia. Materiał i metody. Analizy mikrobiologiczne obejmowały określenie liczebności bakterii mezofilnych, psychrofilnych, bakterii grupy coli, form kałowych E. coli, a także E. faecalis, C. perfringens, Staphylococcus spp. i Salmonella spp.. Próbki wód pobierane były 4 razy w roku w następujących terminach: 27.04.2015 r. (wiosna), 10.07.2015 r. (lato), 12.10.2015 r. (jesień) i 29.12.2015 r. (zima) w 5 punktach na terenie zalewu. Ponadto, w każdym punkcie pomiarowym mierzono temperaturę wody oraz powietrza. Wyniki. Stwierdzono, że na liczebność badanych drobnoustrojów wpływała zmieniająca się temperatura wody i powietrza oraz użytkowanie w sezonie wakacyjnym zalewu w charakterze kąpieliska przez okolicznych mieszkańców. W pobranych próbkach zidentyfikowano wszystkie badane wskaźniki czystości mikrobiologicznej wód, które stanowią potencjalne zagrożenie dla kąpiących się w zbiorniku ludzi. Wnioski. Z epidemiologicznego punktu widzenia zasadnym jest objęcie wód Zalewu w Nowej Hucie stałym monitoringiem sanitarnym.
    Źródło:
    Medycyna Środowiskowa - Environmental Medicine; 2016, 19, 4; 19-26
    1505-7054
    2084-6312
    Pojawia się w:
    Medycyna Środowiskowa - Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of SOS-induced Pol II, Pol IV, and Pol V DNA polymerases on UV-induced mutagenesis and MFD repair in Escherichia coli cells.
    Autorzy:
    Wrzesiński, Michał
    Nowosielska, Anetta
    Nieminuszczy, Jadwiga
    Grzesiuk, Elżbieta
    Powiązania:
    https://bibliotekanauki.pl/articles/1041471.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    MFD
    polB
    UV induced mutagenesis
    E. coli
    dinB
    mutD5
    umuDC
    Opis:
    Irradiation of organisms with UV light produces genotoxic and mutagenic lesions in DNA. Replication through these lesions (translesion DNA synthesis, TSL) in Escherichia coli requires polymerase V (Pol V) and polymerase III (Pol III) holoenzyme. However, some evidence indicates that in the absence of Pol V, and with Pol III inactivated in its proofreading activity by the mutD5 mutation, efficient TSL takes place. The aim of this work was to estimate the involvement of SOS-inducible DNA polymerases, Pol II, Pol IV and Pol V, in UV mutagenesis and in mutation frequency decline (MFD), a mechanism of repair of UV-induced damage to DNA under conditions of arrested protein synthesis. Using the argE3→Arg+ reversion to prototrophy system in E. coli AB1157, we found that the umuDC-encoded Pol V is the only SOS-inducible polymerase required for UV mutagenesis, since in its absence the level of Arg+ revertants is extremely low and independent of Pol II and/or Pol IV. The low level of UV-induced Arg+ revertants observed in the AB1157mutD5ΔumuDC strain indicates that under conditions of disturbed proofreading activity of Pol III and lack of Pol V, UV-induced lesions are bypassed without inducing mutations. The presented results also indicate that Pol V may provide substrates for MFD repair; moreover, we suggest that only those DNA lesions which result from umuDC-directed UV mutagenesis are subject to MFD repair.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 1; 139-147
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Using multitype branching models to analyze bacterial pathogenicity
    Autorzy:
    Tahir, Daniah
    Kaj, Ingemar
    Bartoszek, Krzysztof
    Majchrzak, Marta
    Parniewski, Pawel
    Sakowski, Sebastian
    Powiązania:
    https://bibliotekanauki.pl/articles/747904.pdf
    Data publikacji:
    2020
    Wydawca:
    Polskie Towarzystwo Matematyczne
    Tematy:
    Markov models
    branching processes
    limit theorems
    virulence factors
    E. coli strains
    Opis:
    We apply multitype, continuous time, Markov branching models to study pathogenicity in E. coli, a bacterium belonging to the genus Escherichia. First, we examine briefly, the properties of multitype branching processes and we also survey some fundamental limit theorems regarding the behavior of such models under various conditions. These theorems are then applied to discrete, state dependent models, in order to analyze pathogenicity in a published clinical data set consisting of 251 strains of E. coli. We use well established methods, incorporating maximum likelihood techniques, to estimate speciation rates as well as the rates of transition between different states of the models. From the analysis, we not only derive new results, but we also verify some preexisting notions about virulent behavior in bacterial strains.
    Źródło:
    Mathematica Applicanda; 2020, 48
    1730-2668
    2299-4009
    Pojawia się w:
    Mathematica Applicanda
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bacterial recognition of thermal glycation products derived from porcine serum albumin with lactose
    Autorzy:
    Sarabia-Sainz, Andre-i
    Ramos-Clamont, Gabriela
    Winzerling, Joy
    Vázquez-Moreno, Luz
    Powiązania:
    https://bibliotekanauki.pl/articles/1039957.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    neoglycans
    bacterial recognition
    E. coli K88 adhesion
    glycation of serum albumin
    Opis:
    Recently, glyco-therapy is proposed to prevent the interaction of bacterial lectins with host ligands (glycoconjugates). This interaction represents the first step in infection. Neoglycans referred to as PSA-Lac (PSA-Glu (β1-4) Gal) were obtained by conjugation of porcine serum albumin (PSA) with lactose at 80 °C, 100 °C and 120 ºC. Characterization studies of the products showed that PSA could contain 1, 38 or 41 added lactoses, depending on the reaction temperature. These neoglycans were approximately 10 times more glycated than PSA-Lac obtained in previous work. Lactose conjugation occurred only at lysines and PSA-Lac contained terminal galactoses as confirmed by Ricinus communis lectin recognition. Furthermore, Escherichia coli K88+, K88ab, K88ac and K88ad adhesins showed affinity toward all PSA-Lac neoglycans, and the most effective was the PSA-Lac obtained after 100 ºC treatment. In vitro, this neoglycan partially inhibited the adhesion of E. coli K88+ to piglet mucin (its natural ligand). These results provide support for the hypothesis that glycated proteins can be used as an alternative for bioactive compounds for disease prevention.
    Źródło:
    Acta Biochimica Polonica; 2011, 58, 1; 95-100
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Reverse osmosis as one-step wastewater treatment: a case study on groundwater pollution
    Autorzy:
    Ali, W.
    Rehman, W. U.
    Younas, M.
    Ahmad, M. I.
    Gul, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/779416.pdf
    Data publikacji:
    2015
    Wydawca:
    Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
    Tematy:
    reverse osmosis (RO)
    membrane separation
    Total Dissolved Solids (TDS)
    Total Coliform
    E. Coli
    Opis:
    The present case study is focused on performance evaluation of a Reverse Osmosis (RO) membrane based pilot plant to treat the ground/surface drinking water sources polluted by flood, rivers and/or canals. The RO plant was designed, fabricated and then operated with polluted water. Two feed water samples having a TDS of 2.000 mg · L−1 and 10.000 mg · L−1 respectively, were taken and analyzed for physical, chemical and microbiological contaminants. The RO plant was run once through over a span of 100 hrs at an operating pressure of 15 bar. TDS rejections were found to be more than 94% while permeate flux was measured to be 25.82 L · m−2 · hr−1 to 40.55 L · m−2 · hr−1. Results show that RO plant has a potential to remove physical, chemical and microbiological contaminants like Total Coliform and E. Coli in one step.
    Źródło:
    Polish Journal of Chemical Technology; 2015, 17, 4; 42-48
    1509-8117
    1899-4741
    Pojawia się w:
    Polish Journal of Chemical Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Synthesis of some Novel Halogenated Platinum (II) Complexes of Active Schiff’s Base Ligand Derived from 5-Bromo Isatin and Evaluation of their Antibacterial Activity
    Autorzy:
    Patange, Ashok N.
    Yadav, Uttam M.
    Desai, Pratik A.
    Singare, Pravin U.
    Powiązania:
    https://bibliotekanauki.pl/articles/1194025.pdf
    Data publikacji:
    2015
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    5-bromo Isatin
    Bacillus pumilus
    E. Coli
    Micrococcus Lutes
    Salmonella abony
    Schiff’s base
    Semicarbazone
    Thiosemicarbazone
    Opis:
    This paper presents the synthesis and antibacterial studies some novel halogenated Platinum (II) complexes of active Schiff’s base ligands of the type [Pt(L1H)2]Cl2, [Pt(L2H)2]Cl2 and[Pt(L)2] derived from 5-Bromo Isatin. Where L1H= hydrazinecarbothiamide of 5-bromoindolinedione and L2H = hydrazinecarboxamide of 5-bromoindolinedione. All complexes reported here had been characterised by 1H NMR and IR spectral studies. Molar conductance studies support the 1:2 natures of these complexes. Pt (II) complexes are diamagnetic in nature and showing square planner geometry. The IR spectral data reveals that both the Schiff’s bases (L1H and L2H) behave as bidentate ligands and are co-ordinated to Pt (II) metal through the sulfur and hydrogenic nitrogen atom.all the new synthesized compounds were screened for antibacterial activity against four of the test organisms like Bacillus pumilus, Micrococcus Lutes, E. coli and Salmonella abony
    Źródło:
    World Scientific News; 2015, 10; 32-43
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    LBP gene methylation involved in mRNA expression and resistance to E. coli F18 in weaned piglets
    Autorzy:
    Gan, L.N.
    Bao, W.B.
    Wu, S.L.
    Qin, W.Y.
    Sun, L.
    Zhao, C.X.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087872.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    LBP gene
    methylation analysis of the LBP region
    E.coli F18 strain
    weaned piglets
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 4; 643-650
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Spontaneous mutagenesis in exponentially growing and stationary-phase, umuDC-proficient and -deficient, Escherichia coli dnaQ49.
    Autorzy:
    Nowosielska, Anetta
    Nieminuszczy, Jadwiga
    Grzesiuk, Elżbieta
    Powiązania:
    https://bibliotekanauki.pl/articles/1041546.pdf
    Data publikacji:
    2004
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    dnaQ49
    argE3→Arg+ revertants
    E. coli
    Pol V
    spontaneous mutagenesis
    stationary-phase mutations
    Opis:
    Spontaneous mutations arise not only in exponentially growing bacteria but also in non-dividing or slowly dividing stationary-phase cells. In the latter case mutations are called adaptive or stationary-phase mutations. High spontaneous mutability has been observed in temperature sensitive Escherichia coli dnaQ49 strain deficient in 3'→5' proofreading activity assured by the ε subunit of the main replicative polymerase, Pol III. The aim of this study was to evaluate the effects of the dnaQ49 mutation and deletion of the umuDC operon encoding polymerase V (Pol V) on spontaneous mutagenesis in growing and stationary-phase E. coli cells. Using the argE3OC →Arg+ reversion system in the AB1157 strain, we found that the level of growth-dependent and stationary-phase Arg+ revertants was significantly increased in the dnaQ49 mutant at the non-permissive temperature of 37°C. At this temperature, in contrast to cultures grown at 28°C, SOS functions were dramatically increased. Deletion of the umuDC operon in the dnaQ49 strain led to a 10-fold decrease in the level of Arg+ revertants in cultures grown at 37°C and only to a 2-fold decrease in cultures grown at 28°C. Furthermore, in stationary-phase cultures Pol V influenced spontaneous mutagenesis to a much lesser extent than in growing cultures. Our results indicate that the level of Pol III desintegration, dependent on the temperature of incubation, is more critical for spontaneous mutagenesis in stationary-phase dnaQ49 cells than the presence or absence of Pol V.
    Źródło:
    Acta Biochimica Polonica; 2004, 51, 3; 683-692
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Screening for ESβL producing E. coli isolated from clinical urine samples collected from different places of Kalaburagi city
    Autorzy:
    Ramachar, Raghavendra
    Gaddad, Subhashchandra M.
    Shivannavar, Channappa T.
    Powiązania:
    https://bibliotekanauki.pl/articles/1177430.pdf
    Data publikacji:
    2018
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Antibiogram
    Double-disc diffusion test
    ESβL
    Green Metallic Sheen
    Phenotypic detection
    Uropathogenic E. coli
    Opis:
    Bacteria are capable of invading and infecting humans, leading to disease and sometimes death. Different body tissues, organs and systems are vulnerable to different organisms. This study was aimed to isolate and identify the bacteria causing Urinary Tract Infection (UTI) in different age groups and also screened for ESβL production. The mid-stream urine samples showing symptoms of urinary tract infections were collected in a wide container from different hospitals and diagnostic centres of Kalaburagi city. The isolation of uropathogen was done by semi-quantitative method of inoculating the samples on the selective and differential media such as Eosin Methylene Blue (EMB) and MacConkey (MAC) agar media respectively. The isolated pathogen was identified by conventional methods like cultural, morphological and biochemical tests. The antibiotic susceptibility test was carried out by Kirby-Bauer disc diffusion technique and ESβL production by double disk-diffusion test (DDDT) as per CLSI guidelines (2013). Out of 550 samples screened, a total of 288 bacteria were isolated of those half of them (146) were E. coli isolates and all were found to be multidrug resistant and half of them (50%) were of ESβL producers. All ESβL producing E. coli isolates were resistant to ceftazidime and exhibited higher level of resistance to Cephalothin, Erythromycin, Cotrimoxazole and Aztreonam. As ESβL producing organisms limits the available treatment options, so, the timely administration of sensitive antibiotic and avoiding antibiotic abuse will help in curing the disease without going for the costly drugs such as Carbapenems. Our study revealed that nitrofurantoin, ofloxacin and cefoxitin may be considered as drug of choice for the treatment of UTI patients. The ESβL production in uropathogens should be continuously monitored in the clinics and hospitals as to avoid the emergence of more multi drug resistance strains.
    Źródło:
    World Scientific News; 2018, 103; 107-118
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Estimation of significance of AlkB and AlkA proteins in DNA repair in Escherichia coli model
    Autorzy:
    Sokołowska, B.
    Maciejewska, A. M.
    Jóźwik, A.
    Kuśmierek, J. T.
    Powiązania:
    https://bibliotekanauki.pl/articles/333316.pdf
    Data publikacji:
    2011
    Wydawca:
    Uniwersytet Śląski. Wydział Informatyki i Nauki o Materiałach. Instytut Informatyki. Zakład Systemów Komputerowych
    Tematy:
    rozpoznawanie obrazów
    klasyfikator najbliższych sąsiadów
    analiza cech pracy odbiornika
    naprawa DNA
    adaptacyjna odpowiedź
    powstawanie mutacji
    bakterie e.coli
    pattern recognition
    fuzzy k-NN classifier
    ROC analysis
    DNA repair
    adaptive response
    mutagenesis
    AlkB dioxygenase
    AlkA glycosylase
    E.coli
    Opis:
    The paper concerns estimation of significance of differences of mutagenesis level between the wild-type strain (wt) and its derivatives which differ in DNA repair ability, namely alkA and alkB strain, devoided AlkA glycosylase and AlkB dioxygenase activity, respectively. The strains were analyzed for their ability to repair 1,N6-ethenoadenine (εA) - chloroacetaldehyde adduct to DNA. The analysis was done using classical statistical and pattern recognition methods. The obtained results confirmed that AlkB dioxygenase plays the most important role in εA repair in E. coli in the experimental modeling.
    Źródło:
    Journal of Medical Informatics & Technologies; 2011, 17; 321-326
    1642-6037
    Pojawia się w:
    Journal of Medical Informatics & Technologies
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The Effect of Garlic Extract Addition on Tilapia Skin Gelatin Based Edible Coating Towards Antimicrobial Properties and Fish Meatball’s Shelf Life
    Autorzy:
    Reynaldi, Ahmad
    Rostini, Iis
    Afrianto, Eddy
    Rochima, Emma
    Powiązania:
    https://bibliotekanauki.pl/articles/1058047.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    E. coli
    Pseudomonas aeruginosa
    Salmonella typhimurium
    Staphyllococcus aureus
    TPC
    edible coating
    extract
    garlic
    pH values
    shelf life
    tilapia gelatin
    Opis:
    The purpose of this research is to determine the best concentration and effect of garlic extract incorporated on tilapia skin gelatin-based edible coating to preserve and extend the shelf life of fish meatball at cold storage. This research was conducted from May 2018 until October 2018 on Fisheries Product Processing Laboratory of The Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. The garlic extract conducted with maceration method with ethanol 96% was performed at Organic Chemical Laboratory FMIPA Universitas Padjadjaran, and for TPC test, inhibition test of edible coating Tilapia’s fish-skin with garlic extract was conducted in Microbiology and Molecular Biotechnology Laboratory of Faculty of Fisheries and Marine Sciences Universitas Padjadjaran. The method used is experimental method with 5 treatments which is meatballs without edible coating. The addition of garlic extract was done with various concentration at 0%, 1%, 1.5%, 2%. The observations were Total Plate Count (TPC) test, inhibition zone test against Pseudomonas aeruginosa, and pH value. The results showed that the best treatment is 2% garlic extract with TPC value 1.1×105 cfu/g during 14 days of storage and concluded that it could extend the shelf life of meatball until 14 days, pH value was 7.03 and inhibition zone against P. aeruginosa was 7.01 mm. The addition of garlic extract on tilapia skin gelatin-based edible coating could extend the shelf life of fish meatballs until 14 days on cold storage.
    Źródło:
    World Scientific News; 2019, 134, 2; 74-85
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Water quality and microbial contamination status of groundwater in Jaffna Peninsula, Sri Lanka
    Jakość wody i mikrobiologiczne zanieczyszczenie wód gruntowych na półwyspie Jaffna na Sri Lance
    Autorzy:
    Mahagamage, M. G. Y. L.
    Manage, Pavithrani S.
    Manage, Pathmalal M.
    Powiązania:
    https://bibliotekanauki.pl/articles/292453.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Technologiczno-Przyrodniczy
    Tematy:
    coliform
    E.coli
    groundwater
    Jaffna Peninsula
    Salmonella spp.
    Shigella spp
    water quality
    bakterie z grupy coli
    Escherichia coli
    jakość wody
    półwysep Jaffna
    Shigella spp.
    wody gruntowe
    Opis:
    In Sri Lanka, among 2588 Salmonella positive cases, the highest incidences were recorded from Jaffna peninsula during 2005 to 2013. Therefore, the present study aimed to identify the microbiological and chemical contamination status of groundwater (40 well water) sources in Jaffna during November 2016. The total coliform, E. coli, Salmonella spp. and Shigella spp. along with some physico-chemical parameters of groundwater were studied. The results revealed that entire peninsula was contaminated with total coliform and E. coli bacteria and the parameters recorded were not within the WHO and SLS (Sri Lanka Standards) drinking water quality standards. 38% of sampling locations were positive for Salmonella spp. and among them six sampling locations were being used for drinking purposes. The results of the study correlates with the statistics of typhoid cases recorded in Jaffna. Results of the study also revealed that around 80% of wells were not within the values specified in guidelines of the SLS for drinking water quality on electrical conductivity. Further, 15% of wells recorded greater than 10 mg∙dm–3 nitrate, which is still below the SLS drinking water standards (45 mg∙dm–3). According to the water quality data, PCA analysis showed that Jaffna town, Nallur, Tellippalai and Kopay DS divisions has similar characteristics for water quality.
    Spośród 2588 przypadków zatrucia salmonellą zanotowanych na Sri Lance największą liczbę zachorowań stwierdzono na półwyspie Jaffna w latach 2005–2013. Dlatego prezentowane badania miały na celu identyfikację chemicznego i mikrobiologicznego zanieczyszczenia wód gruntowych z 40 studni w listopadzie 2016 r. Analizowano całkowitą liczbę bakterii z grupy coli, Escherichia coli, Salmonella spp., Shigella spp. oraz wybrane parametry fizyczne i chemiczne wód gruntowych. Wyniki ujawniły, że cały półwysep jest skażony bakteriami z grupy coli i bakterią Escherichia coli, a analizowane parametry nie spełniają standardów WHO i standardów Sri Lanki co do jakości wody. W 38% badanych stanowisk stwierdzono obecność Salmonella spp., przy czym sześć z nich wykorzystywano jako źródła wody pitnej. Wyniki badań korelowały z rejestrowanymi przypadkami duru brzusznego na półwyspie. Stwierdzono także, że 80% studni nie spełniało wymogów stawianych wodzie pitnej przez normy Sri Lanki w odniesieniu do przewodnictwa elektrolitycznego. Ponadto stężenie azotanów w 15% studni było większe niż 10 mg∙dm–3, ale nie przekraczało granicznej wartości 45 mg∙dm–3 wyznaczonej przez normy dla wody pitnej. Analiza PCA wykazała, że miasto Jaffna oraz gminy Nallur, Tellippalai i Kopay mają wody o podobnych właściwościach.
    Źródło:
    Journal of Water and Land Development; 2019, 40; 3-12
    1429-7426
    2083-4535
    Pojawia się w:
    Journal of Water and Land Development
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Expression of the gene encoding blood coagulation factor VIII without domain B in E. coli bacterial expression system
    Autorzy:
    Mazurkiewicz-Pisarek, Anna
    Mazurkiewicz, Alina
    Mikiewicz, Diana
    Baran, Piotr
    Ciach, Tomasz
    Powiązania:
    https://bibliotekanauki.pl/articles/16706242.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    factor VIII
    hemophilia type A
    recombinant coagulation factor VIII
    prokaryotic expression system
    E. coli
    recombinant protein production system
    Opis:
    In this article, we have demonstrated the feasibility of generating an active form of recombinant blood coagulation factor VIII using an E. coli bacterial expression system as a potential treatment for hemophilia type A. Factor VIII (FVIII), an essential blood coagulation protein, is a key component of the fluid phase blood coagulation system. So far, all available recombinant FVIII formulations have been produced using eukaryotic expression systems. Mammalian cells can produce catalytically active proteins with all the necessary posttranslational modifications. However, cultivating such cells is time-consuming and highly expensive, and the amount of the obtained product is usually low. In contrast to eukaryotic cells, bacterial culture is inexpensive and allows the acquisition of large quantities of recombinant proteins in a short time. With this study, we aimed to obtain recombinant blood coagulation factor VIII using the E. coli bacterial expression system, a method not previously explored for this purpose. Our research encompasses the synthesis of blood coagulation factor VIII and its expression in a prokaryotic system. To achieve this, we constructed a prokaryotic expression vector containing a synthetic factor VIII gene, which was then used for the transformation of an E. coli bacterial strain. The protein expression was confirmed by mass spectrometry, and we assessed the stability of the gene construct while determining the optimal growth conditions. The production of blood coagulation factor VIII by the E. coli bacterial strain was carried out on a quarter-technical scale. We established the conditions for isolation, denaturation, and renaturation of the protein, and subsequently confirmed the activity of FVIII.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 3; 247-262
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antibiotic Susceptibility Test of Bacteria Isolated From Fruit Juices Sold in Cafes and Restaurants of Debre-Markos Town, North Western Ethiopia
    Autorzy:
    Geta, Kindu
    Kebede, Ameha
    Chemedissa, Meseret
    Powiązania:
    https://bibliotekanauki.pl/articles/1076786.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Antibiotics
    Antimicrobial resistance
    B. cereus
    Debre Markos
    E. coli
    Enterobacter
    Fruit juices
    Klebsiella
    Pathogens
    Ps. aeruginosa
    Salmonella
    Shigella
    Staphylococcus aureus
    Opis:
    The prevalence of antimicrobial resistance among food pathogens has increased during recent decades. In this work, pathogenic bacteria such as Staphylococcus aureus, Salmonella spp., Shigella spp., and E. coli were isolated following standard methods. The bacterial isolates were then tested for their sensitivity to common antibiotics using the disc diffusion method on Mueller-Hinton Agar. All of the pathogenic bacteria were found to be resistant to erythromycin and almost all were sensitive to penicillin.
    Źródło:
    World News of Natural Sciences; 2019, 24; 365-371
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mismatch dependent uracil/thymine-DNA glycosylases excise exocyclic hydroxyethano and hydroxypropano cytosine adducts.
    Autorzy:
    Borys-Brzywczy, Ewa
    Arczewska, Katarzyna
    Saparbaev, Murat
    Hardeland, Ulrike
    Schär, Primo
    Kuśmierek, Jarosław
    Powiązania:
    https://bibliotekanauki.pl/articles/1041473.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    base excision repair
    E. coli mismatch uracil-DNA glycosylase
    exocyclic cytosine adducts
    human thymine-DNA glycosylase
    S. pombe Thp1p glycosylase
    Opis:
    Exocyclic adducts of DNA bases, such as etheno- and hydroxyalkano- ones, are generated by a variety of bifunctional agents, including endogenously formed products of lipid peroxidation. In this work we selectively modified cytosines in the 5'-d(TTT TTT CTT TTT CTT TTT CTT TTT T)-3' oligonucleotide using: chloroacetaldehyde to obtain 3,N4-α-hydroxyethano- (HEC) and 3,N4-etheno- (εC), acrolein to obtain 3,N4-α-hydroxypropano- (HPC) and crotonaldehyde to obtain 3,N4-α-hydroxy-γ-methylpropano- (mHPC) adducts of cytosine. The studied adducts are alkali-labile which results in oligonucleotide strain breaks at the sites of modification upon strong base treatment. The oligonucleotides carrying adducted cytosines were studied as substrates of Escherichia coli Mug, human TDG and fission yeast Thp1p glycosylases. All the adducts studied are excised by bacterial Mug although with various efficiency: εC >HEC >HPC >mHPC. The yeast enzyme excises efficiently εC ł HEC >HPC, whereas the human enzyme excises only εC. The pH-dependence curves of excision of εC, HEC and HPC by Mug are bell shaped and the most efficient excision of adducts occurs within the pH range of 8.6-9.6. The observed increase of excision of HEC and HPC above pH 7.2 can be explained by deprotonation of these adducts, which are high pKa compounds and exist in a protonated form at neutrality. On the other hand, since εC is in a neutral form in the pH range studied, we postulate an involvement of an additional catalytic factor. We hypothesize that the enzyme structure undergoes a pH-induced rearrangement allowing the participation of Lys68 of Mug in catalysis via a hydrogen bond interaction of its ε-amino group with N4 of the cytosine exocyclic adducts.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 1; 149-165
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Novel synthesis of 3-(((E)-benzylidene)amino)-5-((Z)-3,4-dimethoxybenzylidene)-2-phenyl-3,5-dihydro-4H-imidazol-4-one derivatives as potent antimicrobial agents
    Autorzy:
    Manawar, Rohit B.
    Parmar, Mukesh B.
    Nayaka, Indresh J.
    Pandit, Brinda H.
    Shah, Manish K.
    Powiązania:
    https://bibliotekanauki.pl/articles/1066261.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    4H-imidazol-4-one
    A. niger
    Anti-fungal
    Anti-microbial Agent
    Bioactivity
    C. albicans
    E. coli
    Elemental analysis
    IR spectroscopy
    Mass spectroscopy
    NMR spectroscopy
    P. aeruginosa
    S. aureus
    S. pyogenes
    Opis:
    Novel synthesis of 4H-imidazol-4-one derivative by reaction of (Z)-3-amino-5-(3,4 dimethoxybenzylidene)-2-phenyl-3,5-dihydro-4H-imidazol-4-one with derivative of different aromatic aldehydes and Salicyldehyde. New synthesized compound are thermally stable and neither air-nor moisture sensitive at RT. Successive library of synthesized compound characterized using IR, NMR, Elemental, Mass, and its antimicrobial activity study.
    Źródło:
    World Scientific News; 2019, 130; 42-70
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analiza wstępowania szpitalnych zakażeń układu moczowego w 10-letniej obserwacji oddziału chorób wewnętrznych i nefrologii w Małopolsce
    Autorzy:
    Wałaszek, Marta
    Kołpa, Małgorzata
    Wolak, Zdzisław
    Sydor, Antoni
    Pelczar, Anna
    Powiązania:
    https://bibliotekanauki.pl/articles/2097675.pdf
    Data publikacji:
    2017-06-30
    Wydawca:
    Państwowa Wyższa Szkoła Zawodowa w Tarnowie
    Tematy:
    ddział chorób wewnętrznych
    zakażenie szpitalne
    Healthcare – Associated Infections (HAI)
    szpitalne zakażenie układu moczowego (UTI)
    E. coli
    P. aeruginosa
    internal medicine department
    hospital-acquired infections
    healthcare associated infections
    urinary tract infections
    Opis:
    Wstęp: Zakażenia szpitalne są jednym z poważniejszych zagrożeń zdrowia pacjenta podczas hospitalizacji. Wśród nich zakażenia szpitalne HAI (Healthcare – Associated Infections). Najczęściej występującą formą są szpitalne zakażenia układu moczowego (UTI – Urinary Tract Infection). Cel: W celu zbadania częstości występowania szpitalnych zakażeń układu moczowego poddano analizie strukturę zakażeń szpitalnych występujących u pacjentów hospitalizowanych w Oddziale Chorób Wewnętrznych i Nefrologii Szpitala Wojewódzkiego im. Św. Łukasza w Tarnowie. Materiał i metody: Analizowano dane dotyczące 13 965 pacjentów hospitalizowanych w Oddziale Chorób Wewnętrznych i Nefrologii w latach od 2006 do 2015. W analizie materiału wykorzystano standardowe metody epidemiologiczne i ujednolicone definicje zakażeń szpitalnych wydane przez ECDC (European Center for Disease Prevention and Control) oraz CDC (Centers for Disease Control and Prevention). Wyniki: Wykryto 237 szpitalnych UTI co stanowiło 33% wszystkich zakażeń szpitalnych wykrytych w badanym oddziale. Zachorowalność wynosiła 1,7%, w tym: 1,5% dla potwierdzonych mikrobiologicznie zakażeń (UTI-A: Microbiologically Confirmed Symptomatic UTI) i 0,2% bez potwierdzenia mikrobiologicznego (UTI-B: Not Microbiologically Confirmed Symptomatic UTI). Współczynnik gęstości zachorowań na 1000 osobodni pobytu (ID – density incidence /1000 osobodni pobytu) wynosił 0,2/1000. Rozpoznano 168 przypadków zakażeń UTI związanych z cewnikiem moczowym i 69 przypadków bez cewnika moczowego. Współczynnik gęstości zachorowań dla UTI z cewnikiem moczowym wynosił 3,3 na 1000 osobodni z cewnikiem moczowym. Wśród czynników etiologicznych, które izolowano z materiałów pochodzących od pacjentów z zakażeniem układu moczowego (UTI) dominowały: Escherichia coli 63 (29%), Enterococcus spp. 37 (16%), Klebsiella spp. 23 (11%). Wnioski: Dziesięcioletnia obserwacja szpitalnych UTI występujących w oddziale chorób wewnętrznych i nefrologii wykazała możliwość przeprowadzenia bardzo dokładnej analizy epidemiologicznej tych zakażeń. Porównanie gęstości występowania szpitalnych UTI uzyskane w badanym oddziale z odnotowaną w programach CDC pozwala wnioskować, iż sytuacja epidemiologiczna w badanym oddziale nie odbiega znacząco od sytuacji w innych krajach.
    Introduction: Hospital-acquired infections are one of the most serious health threats during a patient’s stay in hospital, including healthcare associated infections (HAI). The most typical form of hospital-acquired infections is urinary tract infection (UTI). Objective: To examine the frequency of appearing UTIs, the structure of UTIs in in-patients in the department of internal medicine and nephrology at Saint Lucas’s general hospital in Tarnów was analysed. Materials and methods: Data analysis of 13 965 in-patients staying in the department of internal medicine and nephrology from 2006 to 2015 was carried out. To investigate these data epidemiological methods and standard definitions of hospital- acquired infections issued by European Center for Disease Prevention as well as Control and Centers for Disease Control and Prevention were used. Results: 237 hospital-acquired UTIs were revealed, which is 33% of all UTIs revealed in the investigated ward. The UTI incidence rate was 1.7% including 1.5% for microbiologically confirmed symptomatic UTIs and 0.2% not microbiologically confirmed symptomatic UTIs. The incidence density rate per 1 000 person-days was 0.2 over 1 000. The number of revealed catheter-related cases was 168, and not catheter-related cases – 69. The incidence density rate of UTIs associated with urinary catheters was 3.3 per 1 000 person-days. The dominant etiological factors, which were taken to be detected from the infected patients’ specimens, were: Escherichia coli 63 (29%), Enterococcus spp. 37(16%), Klebsiella spp. 23 (11%). Conclusions: A 10 year observation of UTIs, which have appeared in the department of internal medicine and nephrology, allowed to conduct the accurate analysis of these infections. The comparison of urinary tract infection rates done in the investigated ward, and recorded in the Research Participation Programs at the Centers for Disease Control and Prevention (CDC), allows to draw the conclusion that the presented epidemiological situation does not differ significantly from other countries.
    Źródło:
    Health Promotion & Physical Activity; 2017, 2, 3; 141-154
    2544-9117
    Pojawia się w:
    Health Promotion & Physical Activity
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Struktury krzemionkowo-metaliczne – możliwości potencjalnych zastosowań
    Autorzy:
    Wenda, Magdalena
    Zielecka, Maria
    Jeziórska, Regina
    Bujnowska, Elżbieta
    Panasiuk, Marek
    Cyruchin, Krystyna
    Powiązania:
    https://bibliotekanauki.pl/articles/460882.pdf
    Data publikacji:
    2015-03
    Wydawca:
    Mazowieckie Biuro Planowania Regionalnego w Warszawie
    Tematy:
    Escherichia coli
    Staphylococcus aureus
    Salmonella typhimurium
    nanonapełniacze o właściwościach bakteriostatycznych
    Nanomaterials, especially with antimicrobial properties (e.g. coatings and nanocomposites)
    Opis:
    Spośród licznej grupy nanomateriałów szczególną uwagę zwracają nanonapełniacze o właściwościach bakteriostatycznych oraz biobójczych, które stosowane są w tworzywach polimerowych, powłokach, farbach, materiałach medycznych czy tkaninach powlekanych. Krzemionkę o budowie sferycznej, zawierającej różne ilości immobilizowanego nanosrebra, otrzymywano metodą zol – żel w połączeniu z dwustopniową modyfikacją SiO2. Głównymi czynnikami decydującymi o zamierzonym efekcie syntezy jest pH mieszaniny reakcyjnej, szybkość mieszania oraz temperatura. Proponowany sposób otrzymywania krzemionki modyfikowanej nanosrebrem (SiO2-Ag) jest konkurencyjny w stosunku do konwencjonalnych ze względu na unikatowe właściwości tych materiałów. Wbudowanie nanosrebra w strukturę nanokrzemionki zapewnia uzyskanie jej trwałej nanostruktury, co w konsekwencji umożliwia doskonałe zdyspergowanie nanocząstek w osnowie polimerowej. Powtarzalne właściwości nanokrzemionki są zgodne z oczekiwaniami i gwarantują przy tym wysoką jakość otrzymywanych produktów. Aktywność antymikrobową otrzymanych materiałów oceniano wobec bakterii Escherichia coli, Staphylococcus aureus oraz Salmonella typhimurium. Nanometryczny rozmiar cząstek wpływa na zwiększenie ich aktywności biologicznej i chemicznej. Wskazuje to na wzmożoną skuteczność nanocząstek wobec mikroorganizmów.
    Nanomaterials, especially with antimicrobial properties (e.g. coatings and nanocomposites) are presently in great demand with increasing interest for application due to their diversity, unique properties, and seemingly limitless uses. They are currently in commercial use and new ones keep coming on the market. Our study is related to a new group of nanomaterials, namely silica nanospheres containing immobilized silver NPs (Ag-SiO2), which can be successfully used as a fascinating nanofillers in preparation new class of nanocomposites, additives for architectural paints and impregnates. The original synthesis method of spherical silica nanoparticles (NPs) with immobilized silver NPs allows preparation of uniform and size controllable nanoparticles with improved microbiological activity of the Ag-SiO2 containing composite powders. Due to extensive works that are being conducted in our laboratories, mainly based on optimization and modification of silica matrices we achived of a potential novel class of materials with unique antimicrobial properties. Simple and reliable manufacturing technology (sol-gel) for the production of silica containing immobilized nanosilver offers a possibility to control size and uniformity of nanospheres. The properties of obtained by this method silica nanopowders containing immobilized nanometric silver particles are important in the use of such powders as components of polymer nano and micro composites used in conditions favoring growth of bacteria and fungi. They prevent the growth of bacteria in moistened compartments on polymers nanocomposites. Microbilogical examination were performed for Escherichia coli, Staphylococcus aureus and Salmonella typhimurium. The antimicrobial efficiency of these materials increase with decreasing their particle size due to their larger specific area.
    Źródło:
    MAZOWSZE Studia Regionalne; 2015, 16; 141-148
    1689-4774
    Pojawia się w:
    MAZOWSZE Studia Regionalne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cloning of a Rift Valley Fever fusion gene in a plant virus derived replicon vector
    Autorzy:
    Omosimua, Rebecca Oziohu
    Iyappan, Gowtham
    Obembe, Olawole
    Ogunkanmi, Adebayo
    Sathishkumar, Ramalingam
    Powiązania:
    https://bibliotekanauki.pl/articles/1193478.pdf
    Data publikacji:
    2021
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    DNA Cloning
    Escherichia coli DH5α cells
    PBYR2e3K2Mc-GFP
    PBYR2e3K2Mc-RVFV
    RVF disease
    RVFV fusion gene
    bean yellow dwarf derived vector
    emerging disease
    geminivirus vector
    zoonotic disease
    Opis:
    Rift Valley fever (RVF) disease is an emerging viral zoonotic disease caused by Rift Valley fever virus (RVFV). RVF disease is seemingly becoming more severe in endemic populations and in areas of new outbreak. RVF disease is listed by WHO as requiring urgent research and development attention. There is no therapeutics or licensed vaccine for human use in the case of an eventual outbreak. There is a need to clone RVFV genes in cloning vectors which may be useful to produce antigens in plant cells or may be tested directly as DNA vaccines. A synthesized Rift Valley fever virus fusion gene was cloned in a bean yellow dwarf virus derived replicon vector; PBYR2e3K2Mc-GFP by removing and replacing the GFP gene. The cloned PBYR2e3K2Mc-RVFV fusion gene was confirmed by PCR, restriction digestion and DNA sequencing. This cloned RVFV fusion gene in plant virus vector can be used for subsequent protein expression in plants cells or used directly tested as DNA vaccines in future studies.
    Źródło:
    World Scientific News; 2021, 158; 159-172
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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