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Tytuł:
Biochemical activity of di- and polyamines in the green alga Chlorella vulgaris Beijerinck [Chlorophyceae]
Autorzy:
Czerpak, R
Bajguz, A.
Piotrowska, A.
Dobrogowska, R.
Matejczyk, M.
Wieslawski, W.
Powiązania:
https://bibliotekanauki.pl/articles/57046.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
spermine
alga
cell
agmatine
monosaccharide
Chlorella vulgaris
chlorophyll
growth
putrescine
polyamine
diamine
protein
spermidine
Opis:
This study concerns on the influence of diamines (agmatine, putrescine) and polyamines (spermine, spermidine) upon the growth and the content of chlorophyll a and b, monosaccharides and proteins in the cells of alga Chlorella vulgaris Beijerinck (Chlorophyceae). In the experiments agmatine, putrescine, spermine and spermidine in the range of concentrations 10-6-10-3 M were used. At the concentration 10-3 M and the 1st day of cultivation, they have a toxic effect on growth of the algae. It was found that di- and polyamines used within the range of concentration 10-6-10-4 M stimulate the growth and the contents of analysed biochemical parameters in the cells of C. vulgaris. The most stimulating influence on metabolism of the alga was demonstrated by spermidine and putrescine at concentration of 10-4 M. Agmatine and spermine were characterised by a lower biological activity than spermidine and putrescine demonstrated the most stimulating influence.
Źródło:
Acta Societatis Botanicorum Poloniae; 2003, 72, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Black Orlon as promising material for bone tissue engineering
Autorzy:
Parizek, M.
Vetrik, M.
Hruby, M.
Lisa, V.
Bacakova, L.
Powiązania:
https://bibliotekanauki.pl/articles/284127.pdf
Data publikacji:
2014
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
Orlon
polyacrylonitrile
tissue engineering
porous 3D scaffolds
cell adhesion
cell growth
osteoblasts
Źródło:
Engineering of Biomaterials; 2014, 17, no. 128-129; 4-6
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparative analysis of different methodological approaches to the in vitro study of tumour cells chemosensitivity
Autorzy:
Paduch, R
Slotwinska, M.
Stachura, A.
Rzeski, W.
Zdzisinska, B.
Kandefer-Szerszen, M.
Powiązania:
https://bibliotekanauki.pl/articles/961243.pdf
Data publikacji:
2001
Wydawca:
Uniwersytet Marii Curie-Skłodowskiej. Wydawnictwo Uniwersytetu Marii Curie-Skłodowskiej
Tematy:
chemosensitivity
etoposide
tumour cell
drug sensitivity
cisplatin
gelatin sponge
growth
in vitro
Opis:
Drug sensitivity assay was performed using two human tumour celi lines: HeLa and Hep-2 cultivated in two-dimensional monolayer celi cultures and three-dimensional cultures on gelatine sponge SpongostanK. Two cytostatics with different mechanisms of anti-tumour action were used: cisplatin and etoposide. Chemosensitivity of tumour cells was assessed by counting the number of viable and nonviable cells (cytostatic and cytotoxic activity of drugs), by counting the number of apoptotic cells and by clonogenic assay of viable cells. We found that the clonogenic assay was morę sensitive than the other tests used, especially after long-term (7 days) treatment of tumour cells with cytostatics. A short (24h) treatment with cytostatics gave false results which were not confirmed after prolonged treatment with cytostatics. We suppose that short treatment tests should not be used for examination of the chemosensitivity of tumour cells isolated from patients. Tumour cells growing on SpongostanH were viable for a longer time than in monolayer cultures and exhibited chemosensitivity comparable to monolayer celi cultures despite of their multilayer growth on gelatine sponge.
Do badań wrażliwości na cytostatyki użyto dwie ludzkie linie nowotworowe: HeLa i Hep-2, hodowane w formie murawy dwuwymiarowej (płaskiej) oraz w formie przestrzennej, trójwymiarowej, na gąbce żelatynowej Spongostan". W badaniach użyto cytostatyki posiadające różny mechanizm działania przeciwnowotworowego, a mianowicie cisplatynę i etopozyd. Wrażliwość komórek nowotworowych na chemioterapeutyki określano ilością komórek przeżywających i martwych (cytostatyczne i cytotoksyczne właściwości leków), ilością komórek apoptotycznych oraz oceniano klonogenne właściwości komórek przeżywających. Stwierdzono, że ocena klonogennych właściwości komórek jest metodą bardziej czułą w porównaniu z innymi testami, szczególnie po długim (7-dniowym) kontakcie komórek z cytostatykami. Ocena krótkotrwałego (24-godz.) kontaktu komórek nowotworowych z cytostatykami dawała fałszywe wyniki, nie potwierdzone po długotrwałej inkubacji komórek z cytostatykami. Uważamy, że testy polegające na ocenie efektu krótkotrwałej inkubacji komórek z lekami nie powinny być stosowane do oceny wrażliwości na chemioterapeutyki komórek nowotworowych izolowanych od pacjenta. Komórki nowotworowe rosnące na Spongostanie” były żywe dłużej niż rosnące w hodowlach płaskich i pomimo wielowarstwowego wzrostu na gąbce żelatynowej wykazywały wrażliwość na leki porównywalną z hodowlami płaskimi.
Źródło:
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia; 2001, 56
2083-3563
0066-2232
Pojawia się w:
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of Methods in Studies of Cell Death Mechanisms
Autorzy:
Borkowska, A.
Nowakowski, M.
Miszczyk, J.
Lipiec, E.
Wiltowska-Zuber, J.
Rawojć, K.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1030322.pdf
Data publikacji:
2018-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
growth and division
cell processes
fluorescence
optimization
Opis:
While studying the influence of ionizing radiation or certain chemical agents on cells, it is crucial to not only determine cytotoxicity, but also to follow cell death mechanisms. There are different methods to screen processes of cell death and still very important question remains unanswered about differences in results that could be caused by various experimental steps in procedures. Based on literature review two protocols of cell death determination were compared. First protocol regarded collecting cells floating in medium before trypsinization and following centrifugation of them. In the second protocol floating cells were discarded and attached ones were stained and fixed. In all experiments three different untreated cell lines (A172, DU145 as cancer cell lines and in comparison, fibroblasts (FB CCL 110), as a non- cancerous cell line) were used to test applied protocols. Cells were cultured and death processes were examined at different time points up to 120 h. Compared protocols showed statistically significant differences, especially in terms of necrosis, which was higher when included floating cells from culture medium and then centrifuging them. Therefore, presented results show importance of choosing a valid experimental procedure in case of evaluating cells viability and types of cell death pathways quantitatively.
Źródło:
Acta Physica Polonica A; 2018, 133, 2; 263-266
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of iron limitation on cells of the diatom Cyclotella meneghiniana Kützing
Autorzy:
Lewandowska, J.
Kosakowska, A.
Powiązania:
https://bibliotekanauki.pl/articles/47921.pdf
Data publikacji:
2004
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
iron
chlorophyll a
cell
Cyclotella meneghiniana
protein
aquatic ecosystem
Baltic Sea
growth
diatom
limitation
Opis:
The response of the Baltic diatom Cyclotella meneghiniana to iron deficiency was examined. The following growth parameters were measured: cell number, chlorophyll a and protein content. The results demonstrate the ability of this diatom to grow well with minimal iron availability; however, the rate of growth fell markedly at the lowest iron(III) concentration. The results of spectrophotometric chlorophyll a measurements and protein assays using the Lowry and Bradford methods indicated a significant decrease in their quantities. Iron may therefore be an important regulatory factor controlling the growth of diatom C. meneghiniana in an aquatic ecosystem.
Źródło:
Oceanologia; 2004, 46, 2
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of temperature on guaiacol peroxidase of Pyrus communis
Autorzy:
Saeidian, S.
Ghasemifar, E.
Powiązania:
https://bibliotekanauki.pl/articles/10793.pdf
Data publikacji:
2013
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
temperature effect
guaiacol
Peroxidase
Pyrus communis
enzyme
plant growth
plant development
cell wall
Opis:
Peroxidase (EC 1.11.1.7; donor: hydrogen-peroxide oxidoreductase, POD) is one of the key enzymes controlling plant growth, differentiation and development. The enzyme participates in construction, rigidification and eventual lignification of cell walls, biosynthesis of ethylene from 1-aminocyclopropane-1-carboxylic acid and H2O2, regulation of auxin level through auxin catabolism, protection of tissue from damage and infection by pathogenic microorganisms, the oxidation of indoleacetic acid. For peroxidase activity in wild pears extract one pH optimum was observed at 6.5 that probably belong to atleast one isoenzyme. Activity of peroxidase in presence of guaiacol and H2O2 was optimum after incubation at 40 °C. Maximum activity of peroxidase is 300 % .Activity increased to 240 %, 300 %, 70 % and 10 % after 60 minute incubation at 30, 40, 45 and 60 °C for peroxidase. Incubation at high temperature (70 °C) was accompanied with decrease of activity to 10 % peroxidase activity.
Źródło:
International Letters of Natural Sciences; 2013, 05
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of protoporphyrins on production of nitric oxide and expression of vascular endothelial growth factor in vascular smooth muscle cells and macrophages.
Autorzy:
Józkowicz, Alicja
Dulak, Józef
Powiązania:
https://bibliotekanauki.pl/articles/1043649.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cell viability
metalloporphyrins
vascular endothelial growth factor
nitric oxide
heme oxygenase
Opis:
Heme oxygenase-1 (HO-1), an inducible enzyme degrading heme to biliverdin, iron and carbon monoxide, is involved in regulation of inflammation and angiogenesis. Tin protoporphyrin (SnPPIX) and zinc protoporphyrin (ZnPPIX) are commonly used as competitive inhibitors of HO-1. We aimed to compare the effects of SnPPIX and ZnPPIX on the production of vascular endothelial growth factor (VEGF), activity of inducible nitric oxide synthase (iNOS) and cell viability. All experiments were performed on rat vascular smooth muscle cells and murine RAW264.7 macrophages treated with 3-10 μM protoporphyrins. Some cells were additionally stimulated with IL-1β or with lipopolysaccharide. After a 24 h incubation period SnPPIX and ZnPPIX significantly reduced the generation of VEGF in vascular smooth muscle cells and RAW264.7, both in resting and stimulated cells. The inhibitory potentials of both protoporphyrins on VEGF synthesis were very similar. In contrast, analysis of iNOS activity revealed that results obtained with different HO-1 inhibitors are discrepant. Generation of nitric oxide by iNOS was significantly increased by SnPPIX but strongly decreased by ZnPPIX. Similar differences were observed when cell viability was compared. SnPPIX improved the cell survival rate, whereas the same doses of ZnPPIX exerted some cytotoxic effects. In summary, SnPPIX and ZnPPIX can be used as HO-1 inhibitors in some experimental models. However, these compounds produce also HO-independent effects, which can make the interpretation of experiments very uncertain. Thus the involvement of the HO-1 pathway should be always confirmed by more specific methods.
Źródło:
Acta Biochimica Polonica; 2003, 50, 1; 69-79
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Endogenous nitric oxide proves not to be involved in the inhibition by IL-1beta of TGF-alpha-stimulated proliferation of RGM1 cells
Autorzy:
Ishikawa, M.
Tsukimi, Y.
Ogawa, T.
Okabe, S.
Powiązania:
https://bibliotekanauki.pl/articles/70156.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Fizjologiczne
Tematy:
epidermal growth factor
interleukin 1
interleukin
cell
proliferation
DNA synthesis
nitric oxide
gastric mucosal cell
rat
Źródło:
Journal of Physiology and Pharmacology; 2000, 51, 3
0867-5910
Pojawia się w:
Journal of Physiology and Pharmacology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression of vascular endothelial growth factor and microvessel density in oral squamous cell carcinoma and its correlation with various clinico-pathological parameters
Autorzy:
Panigrahi, Ranjita
Jha, Narendra Kumar
Hota, Subhransu Kumar
Powiązania:
https://bibliotekanauki.pl/articles/38695726.pdf
Data publikacji:
2024-03-30
Wydawca:
Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
Tematy:
microvessel density
oral squamous cell carcinoma
vascular endothelial growth factor
Opis:
Introduction and aim. Angiogenesis, which is accomplished by capillary sprouting, is the process by which new vessels are created from pre-existing ones. In tumor, once their initial blood supply is depleted, a tumour is unable to grow without additional blood flow. Additionally, a tumor’s microvasculature, or microvessel density (MVD), increases along with its capacity to produce angiogenesis. We aimed to observe the relationship between the expression of vascular endothelial growth factor (VEGF) and MVD (using CD34) in oral squamous cell carcinoma (OSCC). Material and methods. The expression of VEGF and CD34 antibodies was analysed using immunohistochemistry method on 50 cases of histopathologically proved OSCC. The expression was correlated with clinicopathological parameters. Results. A significant correlation was observed between VEGF expression and gender, LVSI. No correlation between any other factors and the difference in VEGF expression was statistically significant. Similarly, the MVD expression was not found to be statistically significant in any of the pathological parameters. Conclusion. VEGF positivity as well as MVD were found to be independent of the tumor pathology. Tumor MVD was found to be independent of the expression of VEGF. Further studies in a larger study group may establish a significant association so that antiangiogenic targeted therapy may be initiated.
Źródło:
European Journal of Clinical and Experimental Medicine; 2024, 22, 1; 82-87
2544-2406
2544-1361
Pojawia się w:
European Journal of Clinical and Experimental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improved adhesion and growth of osteoblast-like MG-63 cells in cultures on titanium modified by gold particles
Autorzy:
Parizek, M.
Base, T.
Hruby, M.
Lisa, V.
Bacakova, L.
Powiązania:
https://bibliotekanauki.pl/articles/285778.pdf
Data publikacji:
2013
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
metallic materials
titanium
surgical implants
gold microparticles
cell adhesion
cell growth
Opis:
Metallic materials are important for load-bearing bone implants. The osteointegration of these implants can be improved by appropriate surface modifications. Therefore, we present here a study of the cell growth on titanium surfaces modified with films created from gold microparticles. These particles in the form of microplates or polyhedral microcrystals were deposited on titanium plates from ethanol solutions, dried and annealed with a hydrogen flame. Some samples were additionally modified by polyethylene imine. The materials engendered from these modifications were used to investigate the adhesion and growth of human osteoblast-like MG-63 cells on these surfaces in the DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surfaces modified by both types of gold microparticles. This trend was the same three and seven days after seeding. The numbers of cells on pure Ti and Ti modified only with gold particles were significantly higher than on samples which were modified with polyethylene imine. The cell spreading areas projected on the materials were significantly larger in cells on the samples with polyethylene imine modification. However, the shape of these cells was mostly rounded or star-like with thin and long protrusions, while on the materials without polyethylene imine, it was mostly polygonal. The cell proliferation activity was estimated from XTT test, based on the activity of mitochondrial enzymes. This test showed that the proliferation activities of osteoblast-like MG-63 cells of the 3rd and 7th days of the experiment were more pronounced on the samples modified only by gold microparticles. Immunofluorescence showed that the focal adhesion plaques containing vinculin and the fibers containing β-actin were most apparent, more numerous and more brightly stained in cells on Ti modified by gold microplates and gold polyhedral microcrystals, especially in comparison with the corresponding samples modified with polyethylene imine (Fig. 1). Thus, it can be concluded that the modification of titanium samples by both types of gold microparticles enhanced the adhesion and growth of MG 63 cells.
Źródło:
Engineering of Biomaterials; 2013, 16, no. 122-123 spec. iss.; 77
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improvement of growth parameters of prune callus cultures destined to initiate cell suspensions
Autorzy:
Hanus-Fajerska, E
Powiązania:
https://bibliotekanauki.pl/articles/57881.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
improvement
growth parameter
prune
callus culture
cell suspension
Prunus domestica
growth regulator
proliferation
Opis:
Callus was inducted on wounded leaf explants from shoot tips of a particular Prunus domestica 'Węgierka Zwykła' clone cultivated in vitro. The improvement of Sweet Common Prune stock callus tissue parameters has been approached by experiments on culture protocols. Either for the induction or maintenance of tissue modified Murashige and Skoog medium, supplemented with different auxins and cytokinins at varying concentrations, was used. The goal was to obtain the highiest possible proliferative capacity of friable tissue without any signs of cell redifferentiation for about 10 weeks. The choice of auxin was an important factor regulating the rate and kind of tissue growth, and for the examined prune clone auxin alone brought a relatively small proportion of cells into division, so advantageous was to combine it with oxygenated cytokinin. Friable tissue was obtained on media supplemented with dicamba or with picloram, but not with 2.4-D neither alone nor combinated with IBA.
Źródło:
Acta Societatis Botanicorum Poloniae; 2006, 75, 1; 5-9
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of in vitro culture conditions influencing the initiation of raspberry (Rubus idaeus L. cv. Nawojka) cell suspension culture
Autorzy:
Dziadczyk, Ewa
Domaciuk, Marcin
Dziadczyk, Piotr
Pawelec, Iwona
Szczuka, Ewa
Bednara, Józef
Powiązania:
https://bibliotekanauki.pl/articles/763806.pdf
Data publikacji:
2013
Wydawca:
Uniwersytet Marii Curie-Skłodowskiej. Wydawnictwo Uniwersytetu Marii Curie-Skłodowskiej
Tematy:
cell suspension culture
callus culture
raspberry
Rubus idaeus
plant growth regulators
kultura zawiesiny komórkowej
kultura kalusa
regulatory wzrostu roślin
Opis:
The purpose of our investigation was to determine appropriate conditions for induction of raspberry (Rubus idaeus cv. Nawojka) cell suspension culture. The established callus culture obtained from leaf explants was used as an inoculum for cell culture initiation. Five combinations of plant growth regulators: 1) 4.0 mg l-1 IAA and 1.0 mg l-1 BAP; 2) 0.25 mg l-1 2,4-D; 3) 0.5 mg l-1 2,4-D; 4) 2.0 mg l-1 NAA and 2.0 mg l-1 BAP; 5) 4.0 mg l-1 NAA and 2.0 mg l-1 BAP, added into modified Murashige and Skoog (1962) medium, were tested in order to get the callus culture suitable for initiation of a cell suspension. The best callus (vigorously growing, healthy and friable) was obtained on the medium supplemented with 4.0 mg l-1 IAA and 1.0 mg l-1 BAP. To find the appropriate culture conditions for dispersing callus tissue in liquid medium into single cells and small aggregates, four combinations of plant hormones (auxins and cytokinins) were tested. The best culture medium for induction of raspberry cv. Nawojka cell suspension appeared to be the one supplemented with 1.0 mg l-1 2,4-D. Also the medium with 8.0 mg l-1 IAA and 1.0 mg l-1 BAP was similarly efficient.
Celem prezentowanych badań była optymalizacja warunków kultury in vitro umożliwiających uzyskanie zawiesiny komórkowej maliny (Rubus idaeus L.). Jako inokulum do zainicjowania kultury zawiesinowej zastosowano ustabilizowaną kulturę kalusa uzyskaną z eksplantatów liściowych na zmodyfikowanej pożywce wg Murashige i Skooga (1962). W pierwszym etapie badań testowano 5 kombinacji regulatorów wzrostu (auksyn i cytokinin) dodawanych do pożywki stymulującej powstawanie tkanki kalusowej, w celu uzyskania kultury kalusa odpowiedniej do indukcji zawiesiny komórkowej. Najlepszą tkankę kalusową (szybko mnożącą się, o luźnej strukturze) uzyskano w kombinacji uwzględniającej uzupełnienie pożywki do kultury in vitro auksyną IAA w stężeniu 4,0 mg l-1 oraz cytokininą BAP w stężeniu 1,0 mg l-1 . W drugim etapie badań testowano warunki kultury in vitro umożliwiające odpowiednią dyspersję tkanki kalusowej w płynnej pożywce, skutkującą uzyskaniem populacji pojedynczych komórek oraz małych agregatów komórkowych w hodowli. W tym celu testowano 4 warianty składu pożywki, różniące się rodzajem i stężeniem zastosowanych hormonów roślinnych należących do klasy auksyn i cytokinin. Najlepszy wynik uzyskano w płynnej pożywce uzupełnionej syntetyczną auksyną 2,4-D w stężeniu 1,0 mg l-1, także pożywka zawierająca auksynę IAA w stężeniu 8,0 mg l-1 oraz cytokininę BAP w stężeniu 1,0 mg l-1 dała dobry wynik.
Źródło:
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia; 2013, 68, 2
2083-3563
0066-2232
Pojawia się w:
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Osteoblasts response to novel chitosan/agarose/hydroxyapatite bone scaffold – studies on MC3T3-E1 and hFOB 1.19 cellular models
Autorzy:
Kazimierczak, Paulina
Vivcharenko, Vladyslav
Truszkiewicz, Wiesław
Wójcik, Michał
Przekora, Agata
Powiązania:
https://bibliotekanauki.pl/articles/284277.pdf
Data publikacji:
2019
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
bone tissue engineering
biocompatibility
osteoconductivity
cell growth
osteogenic differentiation
Opis:
Since it is known that various cell lines may ex-press different behaviours on the scaffolds surface, a comprehensive analysis using various cellular mo-dels is needed to evaluate the biomedical potential of developed biomaterials under in vitro conditions. Thus, the aim of this work was to fabricate bone scaffolds composed of a chitosan-agarose matrix reinforced with nanohydroxyapatite and compare the biological response of two cell lines, i.e. mouse calvarial preosteoblasts (MC3T3-E1 Subclone 4) and human foetal osteoblasts (hFOB 1.19). Within this study, the osteoblasts number on the scaffold surface and the osteogenic markers level produced by MC3T3-E1 and hFOB 1.19 cells were determined. Furthermore, changes in calcium and phosphorous ions concentrations in the culture media dedicated for MC3T3-E1 and hFOB 1.19 were estimated after the biomaterial incubation. The obtained results proved that the fabricated biomaterial is characterized by biocompatibility and osteoconductivity since it favours osteoblasts attachment and growth. It also supports the production of osteogenic markers (collagen, bALP, osteocalcin) by MC3T3-E1 and hFOB 1.19 cells. Interestingly, the developed biomaterial exhibits different ion reactivity values in the two culture media dedicated for the mentioned cell lines. It was also revealed that mouse and human osteoblasts differ in the cellular response to the fabricated scaffold. Thus, the use of at least two various cellular models is recommended to carry out a reliable biological characterization of the novel biomaterial. These results demonstrate that the tested bone scaffold is a promising biomaterial for bone regeneration applications, however further biological and physicochemical experiments are essential to fully assess its biomedical potential.
Źródło:
Engineering of Biomaterials; 2019, 22, 151; 24-29
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phosphorylation sites of HER2/c-erbB-2: role in cell growth and in disease
Autorzy:
Khurshid, Rukhshan
Saleem, Mahjabeen
Gul-e-Raana, -
Akhthar, Muhammad
Powiązania:
https://bibliotekanauki.pl/articles/1039198.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
phosphorylation sites
HER2/c-erbB-2
cell growth and disease
Opis:
The protein kinase c-erbB-2 belongs to the family of receptor tyrosine kinase and is involved in oncogenesis. The present study predicts different phosphorylation sites of HER2/c-erbB-2 which are important in preventing or developing cancer, especially breast cancer. Sequence homology showed highest homology (77%) with epidermal growth factor receptor kinase domain. According to PROSITE search result, active sites of c-erbB-2 are N-lobe (glycine rich phosphate binding loop). Catalytic loop with presumptive catalytically active of Asp108 is phosphorylated by tyrosine protein kinase. A-loop, activation loop, becomes phosphorylated and activates the substrate binding. The study strengthens our knowledge regarding HER2 signaling by the detection of uncharacterized signaling proteins, establishing phosphorylation of an activation loop and helps us to make assumptions about the role of such previously unidentified proteins. On the basis of importance of HER2 in breast cancer as well as in other diseases, this study provides fruitful information for designing new therapeutic strategies.
Źródło:
Acta Biochimica Polonica; 2014, 61, 4; 699-703
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Regulation of nuclear phospholipase C activity.
Autorzy:
Manzoli, Lucia
Billi, Anna
Martelli, Alberto
Cocco, Lucio
Powiązania:
https://bibliotekanauki.pl/articles/1043270.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
nucleus
cell growth
regulation
phospholipase C
Opis:
A body of evidence, linking inositide-specific phospholipase C (PI-PLC) to the nucleus, is quite extensive. The main isoform in the nucleus is PI-PLCβ1, whose activity is up-regulated in response to insulin-like growth factor-1 (IGF-1) or insulin stimulation. Whilst at the plasma membrane this PI-PLC is activated and regulated by Gαq/α11 and Gβg subunits, there is yet no evidence that qα/α11 is present within the nuclear compartment, neither GTP-γ-S nor AlF4 can stimulate PI-PLCβ1 activity in isolated nuclei. Here we review the evidence that upon occupancy of type 1 IGF receptor there is translocation to the nucleus of phosphorylated mitogen-activated protein kinase (MAPK) which phosphorylates nuclear PI-PLCβ1 and triggers its signalling, hinting at a separate pathway of regulation depending on the subcellular location of PI-PLCβ1. The difference in the regulation of the activity of PI-PLCβ1mirrors the evidence that nuclear and cytoplasmatic inositides can differ markedly in their signalling capability. Indeed, we do know that agonists which affect nuclear inositol lipid cycle at the nucleus do not stimulate the one at the plasma membrane.
Źródło:
Acta Biochimica Polonica; 2004, 51, 2; 391-395
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
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