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Tytuł:
Novel designs and technologies for cell engineering
Autorzy:
Ziółkowska, K.
Chudy, M.
Dybko, A.
Brzózka, Z.
Powiązania:
https://bibliotekanauki.pl/articles/115526.pdf
Data publikacji:
2011
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
lab-on-a-chip
multicellular tumor spheroid
cancer
in vitro cell culture
cell engineering
Opis:
Microfluidic devices, such as lab-on-a-chip systems, are highly advantageous for cell engineering and cell based assays. It is a particularly useful approach for development of the in vitro cellular systems mimicking the in vivo environment. In this paper, a novel lab-on-a-chip device for three-dimensional human cell culture and anticancer drug testing is presented. Cells were cultured as Multicellular Tumor Spheroids (MCTS) — the best cancer tumor model developed so far. Diff erent designs were tested and novel technique of microfabrication in poly(dimethylsiloxane) was developed. MCTS were cultured in a system of polymeric microwells, with the network of microfluidic channels for culture medium flow. Design included optimal shear stress and proper nutrients supply for cultured cells. Final design provided MCTS culture for four weeks with the homeostasis-like state achievement, which is characteristic for the in vivo situation.
Źródło:
Challenges of Modern Technology; 2011, 2, 4; 54-60
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Inżynieria komórkowa w systemach lab-on-a-chip
Cell engineering in lab-on-chip systems
Autorzy:
Tomecka, E.
Tokarska, K.
Jastrzębska, E.
Chudy, M.
Brzózka, Z.
Powiązania:
https://bibliotekanauki.pl/articles/172147.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Chemiczne
Tematy:
inżynieria komórkowa
mikrosystemy przepływowe
dwuwymiarowa hodowla komórkowa
trójwymiarowa hodowla komórkowa
cell engineering
microfluidic systems
two-dimensional cell culture
three-dimensional cell culture
Opis:
Lab-on-a-chip systems are promising tools in the field of cell engineering. Microfluidic systems are integrated microlaboratories consisting of many microstructures such as microchannels and microchambers, which can be used for cell analysis and cell culture. Appropriately designed geometry of the chip allows to mimic in vivo conditions. Microsystems enables continuous culture medium perfusion. During cell culture, regulation of the flow rate of medium is possible, which allows to control conditions of the cultivation. In this paper we present a review of microfluidics systems which are used in cell engineering. We describe methods of microsystems fabrication, parameters which influence cell proliferation in microscale and examples of microsystems for cell analysis and cell culturing. Microfluidic systems for maintaining cell culture are mainly fabricated of poly(dimethylsiloxane) (PDMS) and glass, non-toxic materials for cells. The most commonly used method for fabrication of PDMS microsystems is photolithography and replica molding techniques. Cell culture in microsystems can be carried out in two ways: as a two-dimensional (2D) cell culture and three-dimensional (3D) cell culture. In two-dimensional culture cells grow as a monolayer on a flat surface of microchambers or microchannels. Microsystems for two-dimensional cell culture are widely described in the literature. They are mainly used for: (i) cell proliferation after exposure to external stimuli, (ii) testing the activity of cytotoxic drugs, (iii) interactions and cell migration and (iv) the evaluation of procedures applicable in tumor therapy e. g. photodynamic therapy. However, two-dimensional cell culture do not mimic fully in vivo conditions. In living organisms cells grow spatially creating three-dimensional structures like tissues. Therefore, nowadays microsystems for 3D cell culture are being developed intensively. Three-dimensional cell culture in microfluidic systems can be achieved in three ways: by the design of suitable geometry and topography of microchannels, by the use of hydrogels or by spheroids formation. Three-dimensional cell culture in microfluidic systems are much better experimental in vitro models than cell culture in traditional culture vessels. It is the main reason why microsystems should be still improved, as to become widely used research tools in cellular engineering.
Źródło:
Wiadomości Chemiczne; 2015, 69, 9-10; 909-929
0043-5104
2300-0295
Pojawia się w:
Wiadomości Chemiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Use of HIV as a gene transfer vector
Autorzy:
Pluta, Krzysztof
Kacprzak, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/1040467.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Lentivirus
lentiviral vectors
AIDS
HIV-1 life cycle
viral-host protein interactions
gene therapy
animal transgenesis
cell engineering
Opis:
Despite the extensive research efforts over the past 25 years that have focused on HIV, there is still no cure for AIDS. However, tremendous progress in the understanding of the structure and biology of the HIV virus led to the development of safe and potent HIV-based transgene delivery vectors. These genetic vehicles are referred to as lentiviral vectors. They appear to be better suited for particular applications, such as transgene delivery into stem cells, compared to other viral- and non-viral vectors. This is because Lentivirus-based vectors can efficiently infect nondividing and slowly dividing cells. In the present review article, the current state of understanding of HIV-1 is discussed and the main characteristics that had an impact on vector design are outlined. A historical view on the vector concept is presented to facilitate discussion of recent results in vector engineering in a broader context. Subsequently, a state of the art overview concerning vector construction and vector production is given. This review also touches upon the subject of lentiviral vector safety and related topics that can be helpful in addressing this issue are discussed. Finally, examples of Lentivirus-based gene delivery systems and their applications are presented, with emphasis on animal transgenesis and human gene therapy.
Źródło:
Acta Biochimica Polonica; 2009, 56, 4; 531-595
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Industrial engineering education – challenging complexity by simple means
Autorzy:
Gladysz, Bartłomiej
Powiązania:
https://bibliotekanauki.pl/articles/407227.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
engineering education
industrial engineering
layout
line
cell
Opis:
Industrial engineers gather knowledge during their bachelor studies through lectures and practical classes. The goal of practical class might be an extension of knowledge and/or a consolidation and application of already gathered knowledge. It is observed that there exists a gap between theory learnt during lectures and practical classes. If practical classes require holistic approach and solving complex tasks (problems), students strive with understanding relations and connections between parts of knowledge. The aim of this article is to show an example of a simple practical assignment that can serve as a bridge between lectures and practical classes through discussion of interactions and relations between parts of theoretical knowledge. It is an example of in-class simulating of a line and cellular layout considering discussion of elements impacting and impacted by the type of layout (e.g. learning curve, changeovers, etc.). In-class verification of the presented approach confirmed its usability for teaching industrial engineers and bridging the gap between theory delivered through lectures and more advanced practical classes.
Źródło:
Management and Production Engineering Review; 2019, 10, 3; 90--96
2080-8208
2082-1344
Pojawia się w:
Management and Production Engineering Review
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nowe termowrażliwe powierzchnie polimerowe do hodowli i uwalniania komórek skóry
New thermoresponsive polymer layers for skin cell culture and detachment
Autorzy:
Oleszko-Torbus, N.
Utrata-Wesołek, A.
Wałach, W.
Dworak, A.
Trzebicka, B.
Powiązania:
https://bibliotekanauki.pl/articles/171585.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Chemiczne
Tematy:
powierzchnia polimerowa
termowrażliwe powierzchnie polimerowe
biomateriały
warstwa komórkowa
inżynieria warstw komórkowych
inteligentne polimery
polymer surface
thermoresponsive polymer surfaces
biomaterials
cell sheet engineering
intelligent polymers
Opis:
Covalent attachment of a thermoresponsive polymer to solid support leads to layers exhibiting temperature-dependent properties. Below the cloud point temperature (TCP) of the thermoresponsive polymer the layer is hydrophilic – it is hydrated and polymer chains adopt an expanded conformation. Above TCP, the polymer chains collapse due to dehydration and the surface becomes hydrophobic. This is a reversible process, lowering the temperature cause hydration and swelling of the layer. Such thermoresponsive layers can be obtained via reactions of entities present on the surface (e.g. functional groups, radicals etc.) with complementary functionalities in the polymer chains (grafting to) or with monomer subjected to polymerization (grafting from). Thermoresponsive layers may be used in many biomedical applications such as separation of molecules or cell sheet engineering. In this work, well-defined thermoresponsive (co)polymers of glycidol and ethyl glycidyl carbamate (mPGl), 2-ethyl and 2-nonyl-2-oxazoline (PENOx) as well as homopolymers of 2-isopropyl-2-oxazoline (PIPOx) were grafted to functionalized glass and silica substrates with the aim to obtain thermoresponsive layers for potential application in cell sheet engineering. Presence of polymers covalently bonded to substrates was confirmed by FT-IR and XPS studies. The polymer layers were 5-50 nm thick, depending on the molar mass and polymer concentration. Microscopic techniques indicated a smooth surface of mPGl layers, slightly rough texture of PENOx layers and fibrille-like fibers surface of PIPOx layers. Ellipsometry and contact angle studies revealed the response of layers to temperature changes. Biocompatibility of layers with dermal fibroblasts was confirmed by toxicity tests. Thermoresponsive surfaces were employed as substrates for skin cell culture and harvesting. Fibroblasts adhesion and proliferation on investigated surfaces was comparable with control sample. A confluent cell sheet was formed after 24 hours of culture. The influence of surface properties on cell adhesion and proliferation was examined. Detachment of cells from surfaces was controlled by variation of the temperature. An intact monolayer of cultured dermal fibroblasts was detached. No mechanical or enzymatic methods were required to harvest the cell sheets. Skin cell sheets, detached from thermoresponsive polymer layers may be applied in the cell sheet engineering that is highly desirable in tissue regeneration.
Źródło:
Wiadomości Chemiczne; 2016, 70, 11-12; 803-818
0043-5104
2300-0295
Pojawia się w:
Wiadomości Chemiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analiza teoretyczna uzysku energetycznego krzemowych ogniw fotowoltaicznych w warunkach solarnych Małopolski
Theoretical analysis of energy yield for silicon photovoltaic cells in solar conditions in Małopolska Region
Autorzy:
Latała, H.
Kurpaska, S.
Powiązania:
https://bibliotekanauki.pl/articles/292071.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Inżynierii Rolniczej
Tematy:
ogniwo fotowoltaiczne
ogniwo krzemowe
energetyka
photovoltaic cell
silicone cell
power engineering
Opis:
W pracy przeanalizowano teoretyczne możliwości uzysku energii elektrycznej dla panelu fotowoltaicznego wykonanego z krzemu polikrystalicznego. Obliczenia wykonano dla miesięcznych średnich wieloletnich wartości natężenia promieniowania słonecznego dostępnego w warunkach solarnych Małopolski. Do badań wybrano, dostępne na rynku i możliwe do zastosowań w gospodarstwach tego regionu, źródło o mocy 235 Wp.
The work contains an analysis of the theoretical potential of the electric energy yield for a photovoltaic panel made of polycrystalline silicon. Calculations were based on monthly multiannual average values of solar radiation intensity available in solar conditions of Małopolska Region. For their purposes, the researchers have chosen a source with a power reaching 235 Wp, which is available in the market and may be used in farms located in this region.
Źródło:
Inżynieria Rolnicza; 2011, R. 15, nr 4, 4; 183-189
1429-7264
Pojawia się w:
Inżynieria Rolnicza
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The adhesion and growth of vascular smooth muscle cells in cultures on carboranethiol-modified gold films
Autorzy:
Parizek, M.
Base, T.
Londesborough, M. G. S.
Lisa, V.
Bacakova, L.
Powiązania:
https://bibliotekanauki.pl/articles/285409.pdf
Data publikacji:
2008
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
metal coating
gold film
carboranethiol
cell adhesion
cell spreading
cell proliferation
biomaterials
tissue engineering
surgical implants
Opis:
Metal surfaces have become important over the last decade for potential surgical implants, and within this context we present here a study of the cell growth on modified gold surfaces. Gold films, deposited on glass plates and annealed with a hydrogen flame, were modified with four different carboranethiol derivatives: 1-(HS)-1,2-C2B10H11 (A), 1,2-(HS)2-1,2-C2B10H10 (B), 9,12-(HS)2-1,2-C2B10H10 (C) and 1,12-(HS)2-1,12- C2B10H10 (D). The materials engendered from these modifications were used to investigate the adhesion and growth of rat aortic smooth muscle cells cultured on these surfaces in a DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surface of a bare gold film. However, three days after seeding, the number of cells on carboranethiol-modified gold samples B, C and D was significantly higher than the number on a bare gold film. After seven days, the number of cells on a bare gold film and on gold films modified with derivatives A, B and D was very similar, but the surface of a gold film modified with derivative C exhibited a significantly smaller number of cells. This may be explained by the exposure of the CH vertices of the carborane cluster, which are more acidic than the BH vertices exposed toward the cells in either A or B.
Źródło:
Engineering of Biomaterials; 2008, 11, no. 81-84; 117-119
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Black Orlon as promising material for bone tissue engineering
Autorzy:
Parizek, M.
Vetrik, M.
Hruby, M.
Lisa, V.
Bacakova, L.
Powiązania:
https://bibliotekanauki.pl/articles/284127.pdf
Data publikacji:
2014
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
Orlon
polyacrylonitrile
tissue engineering
porous 3D scaffolds
cell adhesion
cell growth
osteoblasts
Źródło:
Engineering of Biomaterials; 2014, 17, no. 128-129; 4-6
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Osteoblasts response to novel chitosan/agarose/hydroxyapatite bone scaffold – studies on MC3T3-E1 and hFOB 1.19 cellular models
Autorzy:
Kazimierczak, Paulina
Vivcharenko, Vladyslav
Truszkiewicz, Wiesław
Wójcik, Michał
Przekora, Agata
Powiązania:
https://bibliotekanauki.pl/articles/284277.pdf
Data publikacji:
2019
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
bone tissue engineering
biocompatibility
osteoconductivity
cell growth
osteogenic differentiation
Opis:
Since it is known that various cell lines may ex-press different behaviours on the scaffolds surface, a comprehensive analysis using various cellular mo-dels is needed to evaluate the biomedical potential of developed biomaterials under in vitro conditions. Thus, the aim of this work was to fabricate bone scaffolds composed of a chitosan-agarose matrix reinforced with nanohydroxyapatite and compare the biological response of two cell lines, i.e. mouse calvarial preosteoblasts (MC3T3-E1 Subclone 4) and human foetal osteoblasts (hFOB 1.19). Within this study, the osteoblasts number on the scaffold surface and the osteogenic markers level produced by MC3T3-E1 and hFOB 1.19 cells were determined. Furthermore, changes in calcium and phosphorous ions concentrations in the culture media dedicated for MC3T3-E1 and hFOB 1.19 were estimated after the biomaterial incubation. The obtained results proved that the fabricated biomaterial is characterized by biocompatibility and osteoconductivity since it favours osteoblasts attachment and growth. It also supports the production of osteogenic markers (collagen, bALP, osteocalcin) by MC3T3-E1 and hFOB 1.19 cells. Interestingly, the developed biomaterial exhibits different ion reactivity values in the two culture media dedicated for the mentioned cell lines. It was also revealed that mouse and human osteoblasts differ in the cellular response to the fabricated scaffold. Thus, the use of at least two various cellular models is recommended to carry out a reliable biological characterization of the novel biomaterial. These results demonstrate that the tested bone scaffold is a promising biomaterial for bone regeneration applications, however further biological and physicochemical experiments are essential to fully assess its biomedical potential.
Źródło:
Engineering of Biomaterials; 2019, 22, 151; 24-29
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electrooxidation of methyl alcohol with Ni-Co catalyst
Autorzy:
Włodarczyk, B.
Włodarczyk, P.
Powiązania:
https://bibliotekanauki.pl/articles/101422.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
Tematy:
methanol
biofuel
Ni-Co catalyst
fuel cell
environment engineering
Opis:
Due to development of the renewable energy sources, the powering of fuel cells (FCs) with bio-fuels is very important. The one of this fuel is methyl alcohol. The use of fuel cells on a large scale is mainly limited by the high cost of catalysts - mainly platinum. Elimination of Pt as catalyst would allow for wider commercial application of FCs. The paper presents a study of methyl alcohol electrooxidation on electrode with NiCo alloy catalyst. Researches were done by the method of polarizing curves of electrooxidation of methanol in glass vessel. Conducted measurements show that there is a possibility of electrooxidation of methanol with Ni-Co catalyst. In any case, the process of electrooxidation of methanol occurs. A maximum current density was equal 50 mA/cm2 . So, the work shows possibility to use Ni-Co alloys as catalysts for fuel electrode to methyl alcohol electrooxidation.
Źródło:
Infrastruktura i Ekologia Terenów Wiejskich; 2018, II/1; 305-315
1732-5587
Pojawia się w:
Infrastruktura i Ekologia Terenów Wiejskich
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Electrooxidation of Coconut Oil in Alkaline Electrolyte
Autorzy:
Włodarczyk, P. P.
Włodarczyk, B.
Powiązania:
https://bibliotekanauki.pl/articles/123509.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
fuel cell
electrooxidation
coconut oil
renewable energy sources
environment engineering
Opis:
Providing more and more energy is an essential task of today's energetic industry. In the last few years, addition to traditional methods of energy production, alternative energy sources have been fast developing. One of the devices that can use these sources is fuel cell. The fuel cells can be a power source of future mainly due to their high efficiency, low influence on environment and possibility of powering with different fuels. Most often fuel cells are powered by hydrogen. However, problems with the problems with its cheap production and storage are the reason for the search of new fuels for fuel cells. But it must be a fuel that will provide zero or low emission level. One of these fuels can be vegetable oil. The paper presents measurements of electrooxidation of coconut oil emulsion on a smooth platinum electrode in an aqueous solution of KOH. Electrochemical measurements were performed in a glass cell with AMEL System 5000 potentiostat. The obtained maximum current density is equal 25 mA/cm2. So, a fundamental possibility of using the coconut oil as fuel for fuel cell. But is necessary to keep the temperature of process above 303K.
Źródło:
Journal of Ecological Engineering; 2017, 18, 5; 173-179
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mice and humans: chromosome engineering and its application to functional genomics.
Autorzy:
Klysik, Jan
Powiązania:
https://bibliotekanauki.pl/articles/1043718.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
gene traps
transgenesis
embryonic stem cell technology
chromosome engineering
ENU mutagenesis
Opis:
Functional modeling of human genes and diseases requires suitable mammalian model organisms. For its genetic malleability, the mouse is likely to continue to play a major role in defining basic genetic traits and complex pathological disorders. Recently, gene targeting techniques have been extended towards developing new engineering strategies for generating extensive lesions and rearrangements in mouse chromosomes. While these advances create new opportunities to address similar aberrations observed in human diseases, they also open new ways of scaling-up mutagensis projects that try to catalogue and annotate cellular functions of mammalian genes.
Źródło:
Acta Biochimica Polonica; 2002, 49, 3; 553-569
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Canola oil electooxitadion on smooth platinum electrode
Autorzy:
Włodarczyk, P.
Włodarczyk, B.
Powiązania:
https://bibliotekanauki.pl/articles/101329.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
Tematy:
canola oil
bio-fuel
fuel cell
renewable energy sources
environment engineering
Opis:
As fuel for fuel cells can be used various substances, but mainly fuel cells are powered by clear hydrogen (or hydrogen obtained from organic substances by reforming process). However, problems with the storage of hydrogen are the reason for the search of new fuels for fuel cells. Due to development of the renewable energy sources, the powering of fuel cells with bio-fuels is very important. Vegetable oil is an alternative fuel for diesel engines and for heating oil burners. Powering high efficiency power sources like fuel cells with renewable fuels (like canola oil) will allow development of renewable energy sources and elimination or reduce of toxic substances emissions. The paper presents the possibility of using canola oil as fuel for direct electricity production. The work shows possible electrooxidation of canola oil emulsion on a smooth platinum electrode in an solution of H2SO4. The resulting current density of canola oil electrooxidation reached the maximum level of 8 mA/cm2. So, the possibility of using canola oil as fuel for direct electricity production has been proved.
Źródło:
Infrastruktura i Ekologia Terenów Wiejskich; 2018, III/1; 589-598
1732-5587
Pojawia się w:
Infrastruktura i Ekologia Terenów Wiejskich
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Vascular smooth muscle cells in cultures on low density polyethylene modified with plasma discharge and biofunctionalization
Autorzy:
Parizek, M.
Kasalkova, N.
Bacakova, L.
Kolarova, K.
Lisa, V.
Svorcik, V.
Powiązania:
https://bibliotekanauki.pl/articles/285087.pdf
Data publikacji:
2009
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
Ar plasma discharge
biomaterials
low-density polyethylene
cell adhesion
cell proliferation
grafting
tissue engineering
vascular smooth muscle cells
Opis:
Low density polyethylene (LDPE) was modified by an Ar plasma discharge and then grafted with glycine (Gly), bovine serum albumin (BSA) or polyethylene glykol (PEG). Some plasma-treated samples and samples grafted with BSA were exposed to a suspension of colloidal carbon particles (C, BSA+C). Pristine LDPE and tissue culture polystyrene dishes (PSC) were used as control samples. The materials were seeded with rat aortic smooth muscle cells and incubated in a medium DMEM with 10% of fetal bovine serum. On day 1 after seeding, the cells on LDPE modified with plasma only, Gly, BSA and BSA+C adhered in similar numbers as on PSC, while the values on non-modified and PEG-modified samples were significantly lower. On day 5, the highest cell numbers were found again on LDPE with Gly, BSA and BSA+C. On day 7, the highest number of cells was found on LDPE modified only with plasma. The latter cells also dis-played the largest cell spreading area. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups after plasma irradiation, and also due to positive effects of grafted Gly, BSA and BSA in combination with colloidal C particles.
Źródło:
Engineering of Biomaterials; 2009, 12, no. 89-91; 25-28
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biotransformacje z udziałem genetycznie modyfikowanych drożdży Saccharomyces cerevisiae
Biotransformations with genetically modified bakers yeast Saccharomyces cerevisiae
Autorzy:
Białecka-Florjańczyk, E.
Zamojska, W.
Kapturowska, A.
Powiązania:
https://bibliotekanauki.pl/articles/171849.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Chemiczne
Tematy:
Saccharomyces cerevisiae
biotransformacja
biokatalizatoy komórkowy
inżynieria genetyczna
biotransformations
whole-cell biocatalysts
genetic engineering
Opis:
Baker’s yeast Saccharomyces cerevisiae is quite commonly applied as a wholecell biocatalysts in biotransformations – reactions based on enzymatic transformations of chemical compounds. Yeast cells are easy in cultivation and use. They are usually used to catalyze such reactions as bioreduction or hydrolysis. The full sequencing of its genome accompanied with achievements of genetic engineering allowed to design new yeast strains characterized by high conversion yield and reaction selectivity. Genetically modified cells of Saccharomyces cerevisiae catalyze biotransformations, which lead to chiral building blocks important in pharmaceutical industry (especially those obtained by reduction of á- and â -oxoesters). „Designer yeast” is a new catalyst for Baeyer–Villiger oxidation. Recombinant yeast lipases have been discussed as useful means in biodiesel production because the microbiological method of producing of this kind of fuel has many advantages. There is a growing interest in application of modified yeast in biotransformation reactions. Modern directions to improve catalytic abilities of baker’s yeast include: the use of surface display technology of enzymes, optimization or increase in availability of cofactor required for bioreduction reactions or gene knock-out, which eliminates the activity of enzymes with conflicting and unwanted stereoselectivities. Commonly used technique is also overexpression of the desired protein or expression of heterologous enzymes in yeast cells.
Źródło:
Wiadomości Chemiczne; 2011, 65, 1-2; 93-110
0043-5104
2300-0295
Pojawia się w:
Wiadomości Chemiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
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