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Tytuł:
Adhesion properties of human bladder cell lines with extracellular matrix components: the role of integrins and glycosylation.
Autorzy:
Lityńska, Anna
Przybyło, Małgorzata
Pocheć, Ewa
Laidler, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1043727.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
glycosylation
integrins
bladder cell lines
adhesion
Opis:
Integrin subunits present on human bladder cells displayed heterogeneous functional specificity in adhesion to extracellular matrix proteins (ECM). The non-malignant cell line (HCV29) showed significantly higher adhesion efficiency to collagen IV, laminin (LN) and fibronectin (FN) than cancer (T24, Hu456) and v-raf transfected (BC3726) cell lines. Specific antibodies to the α2, a5 and β1 integrin subunits inhibited adhesion of the non-malignant cells, indicating these integrin participation in the adhesion to ECM proteins. In contrast, adhesion of cancer cells was not inhibited by specific antibodies to the β1 integrin subunit. Antibodies to α3 integrin increased adhesion of cancer cells to collagen, LN and FN, but also of the HCV29 line with colagen. It seems that α3 subunit plays a major role in modulation of other integrin receptors especially in cancer cells. Differences in adhesion to ECM proteins between the non-malignant and cancer cell lines in response to Gal and Fuc were not evident, except for the v-raf transfected cell line which showed a distinct about 6-fold increased adhesion to LN on addition of both saccharides. N-Acetylneuraminic acid inhibited adhesion of all cell lines to LN and FN irrespective of their malignancy.
Źródło:
Acta Biochimica Polonica; 2002, 49, 3; 643-650
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Adhesive properties of carcinoembryonic antigen glycoforms expressed in glycosylation-deficient Chinese hamster ovary cell lines.
Autorzy:
Krop-Watorek, Anna
Klopocki, Arkadiusz
Czerwinski, Marcin
Lisowska, Elwira
Powiązania:
https://bibliotekanauki.pl/articles/1043841.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
solid-phase cell adhesion assay
N-glycoforms
carcinoembryonic antigen
glycosylation defective mutants
Opis:
Carcinoembryonic antigen (CEA) is an oncofoetal cell surface glycoprotein that serves as an important tumour marker for colorectal and some other carcinomas. Its immunoglobulin-like structure places CEA within the immunoglobulin superfamily. CEA functions in several biological roles including homotypic and heterotypic (with other CEA family members) cell adhesion. Cell-cell interaction can be modulated by different factors, e.g., post-translational modifications such as glycosylation. The purpose of this study was to examine whether changes in carbohydrate composition of CEA oligosaccharides can influence homotypic (CEA-CEA) interactions. In order to modulate glycosylation of CEA we used two different glycosylation mutants of Chinese hamster ovary (CHO) cells, Lec2 and Lec8. Lec2 cells should produce CEA with nonsialylated N-glycans, while Lec8 cells should yield more truncated sugar structures than Lec2. Parental CHO (Pro5) cells and the glycosylation deficient mutants were stably transfected with CEA cDNA. All three CEA glycoforms, tested in a solid-phase cell adhesion assay, showed an ability to mediate CEA-dependent cell adhesion, and no qualitative differences in the adhesion between the glycoforms were observed. Thus, it may be assumed that carbohydrates do not play a role in homotypic adhesion, and the interactions between CEA molecules depend solely on the polypeptide structure.
Źródło:
Acta Biochimica Polonica; 2002, 49, 1; 273-283
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Black Orlon as promising material for bone tissue engineering
Autorzy:
Parizek, M.
Vetrik, M.
Hruby, M.
Lisa, V.
Bacakova, L.
Powiązania:
https://bibliotekanauki.pl/articles/284127.pdf
Data publikacji:
2014
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
Orlon
polyacrylonitrile
tissue engineering
porous 3D scaffolds
cell adhesion
cell growth
osteoblasts
Źródło:
Engineering of Biomaterials; 2014, 17, no. 128-129; 4-6
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cell adhesion molecules and their possible role in the pathology of the peripheral nerves – a review of the literature
Autorzy:
Niezgoda, Adam
Powiązania:
https://bibliotekanauki.pl/articles/552657.pdf
Data publikacji:
2015
Wydawca:
Stowarzyszenie Przyjaciół Medycyny Rodzinnej i Lekarzy Rodzinnych
Tematy:
cell adhesion molecules
demyelination
polyneuropathies.
Źródło:
Family Medicine & Primary Care Review; 2015, 4; 323-326
1734-3402
Pojawia się w:
Family Medicine & Primary Care Review
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of the interactions between human high-molecular-mass kininogen and cell wall proteins of pathogenic yeasts Candida tropicalis
Autorzy:
Karkowska-Kuleta, Justyna
Zajac, Dorota
Bras, Grazyna
Bochenska, Oliwia
Seweryn, Karolina
Kedracka-Krok, Sylwia
Jankowska, Urszula
Rapala-Kozik, Maria
Kozik, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/1038758.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
contact system
kinins
inflammation
candidiasis
cell wall proteins
adhesion
Opis:
Candida tropicalis is one of the most frequent causes of serious disseminated candidiasis in human patients infected by non-albicans Candida species, but still relatively little is known about its virulence mechanisms. In our current study, the interactions between the cell surface of this species and a multifunctional human protein - high-molecular-mass kininogen (HK), an important component of the plasma contact system involved in the development of the inflammatory state - were characterized at the molecular level. The quick release of biologically active kinins from candidal cell wall-adsorbed HK was presented and the HK-binding ability was assigned to several cell wall-associated proteins. The predicted hyphally regulated cell wall protein (Hyr) and some housekeeping enzymes exposed at the cell surface (known as "moonlighting proteins") were found to be the major HK binders. Accordingly, after purification of selected proteins, the dissociation constants of the complexes of HK with Hyr, enolase, and phosphoglycerate mutase were determined using surface plasmon resonance measurements, yielding the values of 2.20 × 10-7 M, 1.42 × 10-7 M, and 5.81 × 10-7 M, respectively. Therefore, in this work, for the first time, the interactions between C. tropicalis cell wall proteins and HK were characterized in molecular terms. Our findings may be useful for designing more effective prevention and treatment approaches against infections caused by this dangerous fungal pathogen.
Źródło:
Acta Biochimica Polonica; 2016, 63, 3; 427-436
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chemical modification of poly ε-caprolactone with wollastonite and its influence on biological properties of osteoblast like-cells MG-63
Autorzy:
Ścisłowska-Czarnecka, A.
Menaszek, E.
Kołaczkowska, E.
Błażewicz, M.
Podporska, J.
Powiązania:
https://bibliotekanauki.pl/articles/284898.pdf
Data publikacji:
2011
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
polycaprolactone
wollastonite
osteoblast-like MG-63 cells
cell adhesion
viability
proliferation
alkaline phosphatase
Opis:
PCL (poly-ε-caprolactone) is a biocompatible and biodegradable polymer of aliphatic polyester group. However, PCL does not effectively bind to the bone in contrast to bioactive inorganic compounds such as wollastonite. For this wollastonite (WS) is regarded as a potential bioactive material for bone tissue engeenering although its main drawback is brittlennes. Therefore we synthesized polymer nanocomposite materials composed of poly-ε-caprolactone and wollastonite (PCL/wollastonite) containing either 0.5% or 5% of the latter modifying filler. And we aimed to verify biological properties of the nanocomposite PCL/WS materials, in comparison to the pure PCL, on cultures of osteoblast-like cells MG-63. The study revealed that the adherence of the osteoblast-like cells to the tested materials was enhanced by the PCL modification (PCL/5WS > PCL/0.5WS > PCL) while cell viability/proliferation was not altered. Furthermore, the activity of alkaline phosphatase indicative of osteoblast differentiation (maturation) was enhanced when the cells were cultured with either PCL/5WS or PCL/0.5WS. Overall, our results indicate that PCL-modified wollastonite improves biological properties of the basic biomaterial suggesting its potential usefulness/application for the bone tissue regeneration.
Źródło:
Engineering of Biomaterials; 2011, 14, 102; 11-14
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improved adhesion and growth of osteoblast-like MG-63 cells in cultures on titanium modified by gold particles
Autorzy:
Parizek, M.
Base, T.
Hruby, M.
Lisa, V.
Bacakova, L.
Powiązania:
https://bibliotekanauki.pl/articles/285778.pdf
Data publikacji:
2013
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
metallic materials
titanium
surgical implants
gold microparticles
cell adhesion
cell growth
Opis:
Metallic materials are important for load-bearing bone implants. The osteointegration of these implants can be improved by appropriate surface modifications. Therefore, we present here a study of the cell growth on titanium surfaces modified with films created from gold microparticles. These particles in the form of microplates or polyhedral microcrystals were deposited on titanium plates from ethanol solutions, dried and annealed with a hydrogen flame. Some samples were additionally modified by polyethylene imine. The materials engendered from these modifications were used to investigate the adhesion and growth of human osteoblast-like MG-63 cells on these surfaces in the DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surfaces modified by both types of gold microparticles. This trend was the same three and seven days after seeding. The numbers of cells on pure Ti and Ti modified only with gold particles were significantly higher than on samples which were modified with polyethylene imine. The cell spreading areas projected on the materials were significantly larger in cells on the samples with polyethylene imine modification. However, the shape of these cells was mostly rounded or star-like with thin and long protrusions, while on the materials without polyethylene imine, it was mostly polygonal. The cell proliferation activity was estimated from XTT test, based on the activity of mitochondrial enzymes. This test showed that the proliferation activities of osteoblast-like MG-63 cells of the 3rd and 7th days of the experiment were more pronounced on the samples modified only by gold microparticles. Immunofluorescence showed that the focal adhesion plaques containing vinculin and the fibers containing β-actin were most apparent, more numerous and more brightly stained in cells on Ti modified by gold microplates and gold polyhedral microcrystals, especially in comparison with the corresponding samples modified with polyethylene imine (Fig. 1). Thus, it can be concluded that the modification of titanium samples by both types of gold microparticles enhanced the adhesion and growth of MG 63 cells.
Źródło:
Engineering of Biomaterials; 2013, 16, no. 122-123 spec. iss.; 77
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improved adhesion and growth of vascular smooth muscle cells in cultures on polyethylene modified by plasma discharge
Autorzy:
Parizek, M.
Kasalkova, N.
Bacakova, L.
Kolarova, K.
Lisa, V.
Svorcik, V.
Powiązania:
https://bibliotekanauki.pl/articles/284295.pdf
Data publikacji:
2007
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
biomateriały
inżynieria tkankowa
Ar plasma discharge
high density and low density polyethylene
cell adhesion
cell proliferation
vascular smooth muscle cells
biomaterials
tissue engineering
Opis:
The attractiveness of synthetic polymers for cell colonization can be affected by physical and chemical modification of the polymer surface. In this study, high density polyethylene (HDPE, m.w. 0.952g/cm3) and low density polyethylene (LDPE, m.w. 0.922g/cm3) were modified by an Ar plasma discharge using Balzers SCD 050 device (exposure time 10, 50, 150 and 400 seconds, discharge power 1.7W). The material was then seeded with rat aortic smooth muscle cells (RASMC; passages 8 to 9, 17 000 cells/cm3) and incubated in a DMEM medium with 10% of fetal calf serum. On day 1 after seeding, the number of initially adhered cells was significantly higher on all modified HDPE and LDPE samples. On day 2, this difference persisted in HDPE, whereas in LDPE only the values on the samples modified by 150 and 400 seconds were significantly higher. On the 5th and 7th day, there were no significant differences in cell number among all LDPE samples. However, on the HDPE foils, significant differences were still apparent on the samples modified for 400 seconds. The cell spreading areas measured on day 1 after seeding were significantly larger on all modified LDPE samples, and, on day 2, on the HDPE samples exposed for 150s. The increased cell colonization was probably due to the formation of oxygen-containing chemical functional groups in the polymer. These results suggest that the responsiveness of the cell to the changes in physiochemical surface properties was more pronounced in HDPE than in LDPE. On both types of polyethylene, the most appropriate exposure time for the enhancement of cell adhesion and growth seemed to be 150 and 400 seconds.
Źródło:
Engineering of Biomaterials; 2007, 10, no. 67-68; 1-4
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of surface topography and morphology of PU/PLA films on fibroblasts performance
Autorzy:
Bednarz, P.
Krok, M.
Laska, J.
Powiązania:
https://bibliotekanauki.pl/articles/285206.pdf
Data publikacji:
2012
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
contact angle
roughness
morphology
cell adhesion
fibroblasts
Źródło:
Engineering of Biomaterials; 2012, 15, no. 116-117 spec. iss.; 120-122
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Integrin αv signaling influences phenotype and maturation of primary human osteoblasts on alumina surface
Autorzy:
Wróbel, E.
Witkowska-Zimny, M.
Mrówka, P.
Głodkowska-Mrówka, E.
Powiązania:
https://bibliotekanauki.pl/articles/284664.pdf
Data publikacji:
2014
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
integrin
human bone-derived cells
osteogenic differentiation
cell adhesion
biomaterial
Opis:
Due to the growing interest in stem cells application in tissue engineering the better understanding of primary human osteoblasts behavior in vitro, on biomaterial surface, is required. Among other molecules integrins may be taken into account as being involved in these phenomena. Integrins are a family of cell adhesion receptors, which may regulate many cellular functions e.g., adhesion, motility, phenotype and cell maturation. The aim of this study was to determine the effect of the biomaterial surfaces and αv integrin signaling pathway on the behavior, phenotype and maturation of human osteoblasts in vitro. Human bone derived cells (HBDCs) obtained from adult femoral bone fragments were cultured on both alumina disks and tissue culture polystyrene (TCPS) dishes. After 7, 14, and 21 days of culture, localization and mRNA expression level of αv integrin subunits and BGLAP (osteocalcin) on polystyrene were analyzed in addition, we treated the cell cultures with monoclonal antibodies against human αv integrin to block its ligand-binding activity, on both alumina and TCPS substrates. We found that the αv integrin was present in focal contacts and cell cytoplasm at subsequent stages of cell maturation and the level of αv integrin mRNA was the highest in mature osteoblasts. Blocking αv integrin transduction pathway caused changes in cell activity and morphology, decreased cells proliferation on TCPS and reduced expression of alkaline phosphatase (ALP) on both materials. The results suggest that αv integrin is involved as an important receptor facilitating osteogenic differentiation.
Źródło:
Engineering of Biomaterials; 2014, 17, 127; 33-39
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molekularne czynniki adhezyjne w szerzeniu się nabłonkowego raka jajnika
Role of molecular adhesion factors in the spread of epithelial ovarian cancer
Autorzy:
Waksmański, Bogdan
Olejek, Anita
Powiązania:
https://bibliotekanauki.pl/articles/908240.pdf
Data publikacji:
2006
Wydawca:
Medical Communications
Tematy:
FAK
cadherins
cell adhesion
epithelial ovarian carcinoma
Opis:
The process of spread of epithelial ovarian cancer is associated with molecular mechanisms including cellular surface receptors, basal membrane components, intercellular adhesion molecules and cell-derived signals. The key role in cellular adhesion and its motility is played by E-cadherin and catenins. Expression of the cadherin – catenin complex decreases in parallel with progressing clinical stage of ovarian cancer. At the same time, re-expression of this complex is associated with dissemination of the neoplasm over peritoneal surface. Changing expression or dysfunction of receptors for integrins and their association with molecules of extracellular matrix (ECM) is closely correlated with tumour progression and/or its metastatic potential. The lysophosphatidic acid mediates laminin-dependant migration of cells by the autocrine local mechanism. The first step in formation of metastases is degradation of collagen type IV, as well as activation of metalloproteinases and pro-angiogenic factors, whose increased expression is seen in ovarian cancer cells. Adhesion contact kinase (FAK) is crucial for migration of neoplastic cells, inversely dependant on growth factors and integrin receptors. Increased expression of this kinase is associated with statistically significant with higher clinical stage of the tumour, presence of metastases in lymph nodes and distant metastases. The basic prerequisite for formation of metastases is derangement of cell adhesion mechanism, inducing loss of adhesion between cells and between cells and extracellular matrix. This mechanism is crucial in the spread of ovarian cancer throughout the abdominal cavity.
Proces szerzenia się nabłonkowego raka jajnika jest związany z mechanizmami molekularnymi obejmującymi receptory powierzchni komórki, składnikami błony podstawnej, międzykomórkowymi cząsteczkami adhezyjnymi oraz sygnałami pochodzącymi od komórki. Podstawową rolę w adhezji komórki i jej ruchliwości pełnią E-kadheryna oraz kateniny. Ekspresja kompleksu kadheryna – kateniny zmniejsza się wraz z postępującym stopniem zaawansowania klinicznego raka jajnika. Jednocześnie występuje reekspresja tego układu w przypadku rozsiewu procesu nowotworowego na powierzchni otrzewnej. Zmieniająca się ekspresja lub nieprawidłowe działanie receptorów integryn i ich związek z cząsteczkami macierzy zewnątrzkomórkowej ECM wykazuje ścisłe połączenie z postępem rozwoju guza lub potencjałem przerzutu. Kwas lizofosfatydowy pośredniczy w zależnej od laminin migracji komórek w autokrynnym mechanizmie lokalnym. Pierwszym krokiem w procesie tworzenia przerzutów jest degradacja kolagenu typu IV z działaniem metaloproteinaz oraz czynników proangiogennych, których zwiększona ekspresja jest obserwowana w komórkach raka jajnika. Kinaza kontaktów adhezyjnych FAK pełni podstawową rolę w migracji komórek nowotworowych ujemnie zależnych od czynników wzrostu oraz receptorów integryn. Zwiększona ekspresja tej kinazy wiąże się istotnie statystycznie z wyższym stopniem zaawansowania klinicznego guza, obecnością przerzutów w węzłach chłonnych i obecnością odległych przerzutów. Podstawowym wstępnym warunkiem powstania przerzutu jest zaburzenie mechanizmu adhezji komórki, co w konsekwencji indukuje zmiany w przyleganiu między komórkami oraz komórkami a macierzą zewnątrzkomórkową. Mechanizm ten ma podstawowe znaczenie w szerzeniu się raka jajnika w obrębie jamy brzusznej.
Źródło:
Ginekologia Onkologiczna; 2006, 4, 3; 193-201
1731-5379
Pojawia się w:
Ginekologia Onkologiczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polish Artificial Heart - new coatings, technology, diagnostics
Autorzy:
Sarna, J.
Kustosz, R.
Major, R.
Lackner, J.
Major, B.
Powiązania:
https://bibliotekanauki.pl/articles/200025.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pulsed laser deposition
microstructure
cell adhesion
Opis:
Since 1991, the Foundation for Cardiac Surgery Development in Zabrze has been implementing research on the artificial heart. In 1995, an artificial ventricle, POLVAD, was implanted to a patient, and in 1998, the prototype of a clinical controller, POLPDU-401, was created. A further development of the studies on an implantable artificial heart requires an integrated approach and an application of advanced methods of materials sciences in order to develop new materials suitable for the contact with blood, as well as to apply a multilateral biomedical diagnostics in hydrodynamic conditions. The estimation of the cell–material interaction plays an important role in the biomaterial design. An analysis of the influence of the carbon content in titanium nitride on the biological and biophysical properties of biomaterial coatings was studied. The cell-material reactions were considered in dynamic and static conditions. Three groups of materials were under examinations – titanium nitride (TiN), as well as titanium carbonitride with a low and high carbon content – of which the best properties were observed for TiN. We found a strong influence of the stoichiometry of TiN (atomic ratio of Ti/N) on the biocompatibility. A non-stoichiometric TiN could have a negative influence on the surrounding tissue.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2010, 58, 2; 329-335
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pro-angiogenic effects of X-rays on murine endothelial cells
Autorzy:
Lisiak, E.
Dziekiewicz, M.
Meineke, V.
Bilski, M.
Janiak, M.
Powiązania:
https://bibliotekanauki.pl/articles/147804.pdf
Data publikacji:
2005
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
angiogenesis
ionizing radiation
cell adhesion
integrins
Opis:
Recently, significant attention has been paid to the possibility of thwarting cancer progression by inhibition of neoangiogenesis (formation of new blood vessels) in growing tumors. Although general mechanisms of angiogenesis have been elucidated, virtually nothing is known about the effects of low doses of ionizing radiation on pro-angiogenic properties of endothelial cells. In the present study, we evaluated the effects of a low (0.2 Gy), intermediate (1 Gy), and high (4 Gy) doses of X-rays on a few angiogenesis-related parameters of isolated murine endothelial cells. We show here that 24 to 48 hours after irradiation with 0.2 Gy the cell proliferation was inhibited to a similar extent as after the exposure to 1 Gy. Also, adhesion of the 0.2 Gy-irradiated cells to both gelatin and MatrigelŽ was inhibited 24 hours post-exposure, whereas irradiation with 1 or 4 Gy resulted in the increased adhesion of the cells to these substrata. Similar effects were observed during the "wound" migration assay. Finally, 24 hours after exposure of the cells to 0.2 Gy of X-rays, the surface expression of the â3 integrin subunit was down-regulated, whereas irradiations with 1 and 4 Gy of X-rays resulted in the significantly elevated expression of this subunit. These results indicate that proliferating endothelial cells are sensitive in vitro to relatively low doses of ionizing radiation
Źródło:
Nukleonika; 2005, 50,suppl.2; 17-20
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Surface properties of neutral components in copper column bioleaching of black shale samples
Autorzy:
Sadowski, Z.
Baranska, J.
Pawlowska, A.
Powiązania:
https://bibliotekanauki.pl/articles/110261.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
adhesion
surface energy
glass beads
biohydrometallurgy
column bioleaching
bacteria cell
polyethylene pellets
Opis:
The behaviour of glass beads and polyethylene pellets as the extra bacteria support in the fine black shale ore in the column bed bioreactor was compared. Capillary rise experiments enabled the calculation of the contact angle and the surface free energy of glass beads and polyethylene pellets. The results showed that the support material made up of polyethylene pellets yielded a higher copper extraction, which could be explained by conditions favourable to cell adhesion on the polyethylene surface.
Źródło:
Physicochemical Problems of Mineral Processing; 2018, 54, 4; 1146-1151
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The adhesion and growth of vascular smooth muscle cells in cultures on carboranethiol-modified gold films
Autorzy:
Parizek, M.
Base, T.
Londesborough, M. G. S.
Lisa, V.
Bacakova, L.
Powiązania:
https://bibliotekanauki.pl/articles/285409.pdf
Data publikacji:
2008
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
metal coating
gold film
carboranethiol
cell adhesion
cell spreading
cell proliferation
biomaterials
tissue engineering
surgical implants
Opis:
Metal surfaces have become important over the last decade for potential surgical implants, and within this context we present here a study of the cell growth on modified gold surfaces. Gold films, deposited on glass plates and annealed with a hydrogen flame, were modified with four different carboranethiol derivatives: 1-(HS)-1,2-C2B10H11 (A), 1,2-(HS)2-1,2-C2B10H10 (B), 9,12-(HS)2-1,2-C2B10H10 (C) and 1,12-(HS)2-1,12- C2B10H10 (D). The materials engendered from these modifications were used to investigate the adhesion and growth of rat aortic smooth muscle cells cultured on these surfaces in a DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surface of a bare gold film. However, three days after seeding, the number of cells on carboranethiol-modified gold samples B, C and D was significantly higher than the number on a bare gold film. After seven days, the number of cells on a bare gold film and on gold films modified with derivatives A, B and D was very similar, but the surface of a gold film modified with derivative C exhibited a significantly smaller number of cells. This may be explained by the exposure of the CH vertices of the carborane cluster, which are more acidic than the BH vertices exposed toward the cells in either A or B.
Źródło:
Engineering of Biomaterials; 2008, 11, no. 81-84; 117-119
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 20

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