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Wyszukujesz frazę "bovine pericardium (BP)" wg kryterium: Temat


Wyświetlanie 1-2 z 2
Tytuł:
Nanostructure of bovine pericardium treated with trypsin
Autorzy:
Turek, A.
Marcinkowski, A.
Trzebicka, B.
Cwalina, B.
Dzierżewicz, Z.
Powiązania:
https://bibliotekanauki.pl/articles/285856.pdf
Data publikacji:
2009
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
nanostructure
bovine pericardium (BP)
collagen fibers
trypsin
Opis:
Various methods of xenogeneic tissues stabilization have been proposed for the purpose of preparing many tissue-derived biomaterials. One of the most important treatments that may lead to obtaining the good-quality tissue biomaterials seems to be decellularization of such tissues. This process may contribute to the reduction of the most frequent failures resulting from the tissues stabilization. The aim of this work was to determine nanostructure of trypsin-treated bovine pericardium, using atomic force microscopy (AFM). The treatment of bovine pericardium with trypsin in EDTA solution resulted in non significant changes in tissue’s morphology. Demonstrated AFM studies of these tissues revealed no failures on the fibers’ surface in the nanoscale. Thus, our results confirm the expec-tation that decellularization may be considered as one of the most promising methods of the allogeneic and xenogeneic tissues stabilization.
Źródło:
Engineering of Biomaterials; 2009, 12, no. 89-91; 30-32
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Radioisotopic investigation of crosslinking density in bovine pericardium used as a biomaterial
Autorzy:
Turek, A.
Cwalina, B.
Kobielarz, M.
Powiązania:
https://bibliotekanauki.pl/articles/147957.pdf
Data publikacji:
2013
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
bioprostheses
bovine pericardium (BP)
crosslinking
methylene blue (MB)
photooxidation
radioisotopic investigations
Opis:
Stabilized bovine pericardium (BP) belongs to tissues routinely applied in production of heart valves. Commercial products are manufactured from tissues crosslinked by glutaraldehyde (GA). Dye-mediated photooxidation was also proposed as an alternative method, which allows for the elimination of clinical failures of GA-treated tissues. The aim of the study was to investigate the density of BP stabilized by GA and the methylene blue-mediated photooxidation, as compared with a native tissue. Crosslinking density was evaluated based on their ability to accumulate radioactive cobalt ions (60Co2+) and the permeability to these ions. Radioactivity was examined using a γ-spectrophotometer (Packard). The results showed the changes in the crosslinking density between the native tissues and photooxidized or GA-crosslinked tissues. Significant decreases in radioactivity were detected only in the thinnest tissues after photooxidation and in filtrates penetrating the same samples. Photooxidized pericardium of a larger thickness did not reveal any significant changes. Weight-dependence for the permeability was observed in the case of filtrates penetrating the GA-treated tissues. However, 60Co2+-accumulation in these samples remained at the same level. Photooxidation may lead to obtaining biomaterials with advantageous properties, i.e. a decreased calcium-binding capacity. Photooxidation efficiencies were dependent both on compactness and thickness of tissues and on process duration. It should be emphasized that the tissues’ structure after photooxidation was characterized by lower density. This may point to the presence of crosslinks of a smaller compactness in comparison with GA treatment. It has been shown that the factor indeed influencing the result of crosslinking is tissue thickness.
Źródło:
Nukleonika; 2013, 58, 4; 511-517
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-2 z 2

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