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Tytuł:
"Battery of Bioassays" for Diagnostics of Toxicity of Natural Water when Pollution with Aluminum Compounds
Autorzy:
Olkova, Anna
Berezin, Grigory
Powiązania:
https://bibliotekanauki.pl/articles/1839077.pdf
Data publikacji:
2021
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
bioassay
aluminum
battery of bioassay
Daphnia magna
Ceriodaphnia affinis
Paramecium caudatum
Escherichia coli
Opis:
The article presents the results of an experimental comparison of the sensitivity of biotests using Daphnia magna Straus, Ceriodaphnia affinis Lilljeborg, Paramecium caudatum Ehrenberg, and Escherichia coli Migula (strain M-17) to water pollution with aluminum compounds. The research was carried out under simulated conditions: the model toxicant was aluminum sulfate Al2(SO4)3∙18H2O, the concentration range per Al was 0.04–2.8 mg/dm3, and the pH of the tested waters was close to the neutral level of 7.2–7.8. The bioluminescence of E. coli significantly decreased at an Al concentration of 0.8 mg/dm3 (toxicity index was 93.3±1.2, which refers to a high level of toxicity). The reaction of P. caudatum was weaker: a high level of toxicity was achieved at an Al concentration of 2.8 mg/dm3. These doses did not cause the death of D. magna and C. affinis in short-term experiments (28 and 96 hours, respectively). However, in the tests for the chronic toxicity of aluminum, we showed that the doses of 0.8 and 2 mg/dm3 Al cause high death of individuals (more than 50%) and a significant decrease in the number of offspring. The range of sensitivity of the bioassay methods to water pollution with aluminum turned out to be as follows: bioassay for the bioluminescence of E. coli > bioassay for the changes in chemotaxis of P. caudatum > bioassay for the changes in fertility of D. magna > bioassay for the changes in fertility of C affinis.
Źródło:
Journal of Ecological Engineering; 2021, 22, 2; 195-199
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wykorzystanie testu mikrojądrowego do oceny genotoksyczności odcieków ze składowisk odpadów
Assessing the genotoxicity of landfill leachates with the aid of the micronucleus bioassay
Autorzy:
Kalka, J.
Ośliślok, A.
Surmacz-Górka, J.
Fudala-Książek, S.
Powiązania:
https://bibliotekanauki.pl/articles/237636.pdf
Data publikacji:
2008
Wydawca:
Polskie Zrzeszenie Inżynierów i Techników Sanitarnych
Tematy:
test mikrojądrowy
odcieki
składowisko odpadów
micronucleus bioassay
Opis:
Genotoksyczność odcieków badano za pomocą testu cytogenetycznego na podstawie zmiany częstości powstawania mikrojąder w komórkach korzenia Vicia faba. Próbki odcieków pobrane do badań pochodziły z dwóch składowisk odpadów o różnym czasie eksploatacji, znajdujących się na terenie Górnośląskiego Okręgu Przemysłowego (Gliwice, Zabrze). Wyniki badań wykazały istotną zależność pomiędzy częstością powstawania mikrojąder i aberracji chromosomowych a stężeniem odcieków wyrażonym jako zawartość rozpuszczonego węgla organicznego. Stwierdzono, że test mikrojądrowy może być skutecznym narzędziem w monitorowaniu genotoksyczności próbek środowiskowych. Uzyskane wyniki wydają się być istotne w świetle toczących się w Komisji Europejskiej dyskusji na temat uzupełnienia wykazu priorytetowych substancji, które w ramach dyrektywy 200/60/WE powinny być identyfikowane i monitorowane w środowisku wodnym.
The cytogenetic biotest was used to examine the genotoxicity of landfill leachates by analyzing the changes in the frequency of micronuclei formation in the cells of Vicia faba root-tips. The leachate samples collected for the purpose of the study came from two landfills differing in age, both situated in the Upper Silesian Industrial District (Gliwice, Zabrze). The results of the study have disclosed a significant relationship between the frequency of micronuclei formation, chromosome aberration and concentrations of the leachates, expressed in terms of organic carbon content. The micronucleus bioassay was found to be a valuable tool for monitoring the genotoxicity of environmental samples. The results obtained can be of significance in the light of the European Commission debates on updating the list of priority substances that are to be identified and monitored in the aquatic environment under the regulations of Directive 200/60/EU.
Źródło:
Ochrona Środowiska; 2008, 30, 4; 15-17
1230-6169
Pojawia się w:
Ochrona Środowiska
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bioremediation Possibilities of Oil-Contaminated Soil by Biosurfactant Based on Bacillus Strain
Autorzy:
Nedoroda, Vladyslav
Trokhymenko, Ganna
Magas, Nataliya
Powiązania:
https://bibliotekanauki.pl/articles/2173345.pdf
Data publikacji:
2022
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
bioassay
biodegradation
bioremediation
biosurfactant
petroleum
phytotoxicity
soil
Opis:
This paper represents the results of the investigation of soil phytotoxicity with a high level of oil pollution. The artificially contaminated soils were exposed to an oil-destroying biosurfactant based on the strains of Bacillus amyloliquefaciens and Bacillus subtilis. The main objective of the work was to evaluate the effectiveness of a consortium of microorganisms in reducing the phytotoxicity of soils with high levels of oil contamination. The determination was performed on the reactions of test organisms to the pollution of the soil environment. The test system in the experiment comprised Sorghum bicolor subsp. Drummondii, Phleum pretense, Galéga officinális, Trifolium pretense, Medicágo sativa, and Sinapis arvensis. The variety of the applied indicator plants has provided a comprehensive analysis of the results of oil destruction and allowed an assessment of the sensitivity of the seeds of each species to toxic substances.
Źródło:
Journal of Ecological Engineering; 2022, 23, 8; 49--55
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bioassay and chemical assay of isoxaflutole residue in soil
Autorzy:
Swarcewicz, M
Bhowmik, P C
Praczyk, T
Skrzypczak, G
Powiązania:
https://bibliotekanauki.pl/articles/802438.pdf
Data publikacji:
2003
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
gas chromatography
soil residue
isoxaflutole herbicide
herbicide
bioassay
Opis:
The objective of this study was to evaluate plant bioassay techniques for various concentrations of isoxaflutole in soil and to compare them with the capillary gas chromatography (GLC). The field experiments with maize were conducted at three locations: South Deerfield, MA (USA), Brody and Winna Góra (Poland) in 1997 and 2000. A silt loam (typical Udifluvents soil) from South Deerfield, was used for the bioassay study. The chemical method was used for two soils from Experiment Stations, Brody and Winna Góra (Poland). The isoxaflutole half-life was 4 days in the Brody soil and 8 days in the South Deerfield soil. The degradation rate of isoxaflutole was very fast during the first ten days after treatment and the second phase of degradation was much slower. The bioassay method was more sensitive than the GLC and showed residues of the herbicide in the South Deerfield soil in 120 DAT. The amount of isoxaflutole residues in Brody soil was 15.3% in 21 DAT, if analyzed by GLC. In Winna Góra soil analyzed by GLC no residue was detected in 19 DAT. Bioassays have advantages over instrumental techniques because they provide more information on the biological significance of the remaining isoxaflutole, and its degradation products in soil. Instrumental methods are more costly and time consuming, as compared to bioassay methods.
Celem badań było opracowanie metody biologicznej oznaczania pozostałości nowego herbicydu isoksaflutolu w glebie z równoczesnym porównaniem uzyskanych wyników za pomocą metody chemicznej. Badania szybkości zanikania isoksaflutolu w glebie prowadzono w 1997 roku w doświadczeniu polowym z kukurydzą w Stacji Doświadczalnej South Deerfield, MA (USA) oraz w 2000 roku w Stacjach Doświadczalnych w Brodach i Winnej Górze. Glebę z South Deerfield oznaczano metodą biologiczną stosując pszenicę (Triticum aestivum L.) i rzepak (Brassica napus L.) jako rośliny testowe, natomiast w Polsce stosowano kapilarną chromatografię gazową. Czas połowicznego zaniku isoksaflutolu wynosił 4-8 dni zarówno przy metodzie biologicznej, jaki i chemicznej. Szybkość zanikania herbicydu w glebie była bardzo duża w pierwszych dziesięciu dniach doświadczenia, a następnie uległa znacznemu zmniejszeniu. Stwierdzono, że metoda biologiczna okazała się bardziej czuła od chemicznej. Metoda chemiczna jest czasochłonna i kosztowna, co stanowi jej ujemną stronę.
Źródło:
Zeszyty Problemowe Postępów Nauk Rolniczych; 2003, 492
0084-5477
Pojawia się w:
Zeszyty Problemowe Postępów Nauk Rolniczych
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of the recombinant cell-based bioassay as a screening method for PCDD/Fs monitoring in fish tissue
Autorzy:
Stypula-Trebas, S.
Warenik, M.
Gembal, M.
Malagocki, P.
Piskorska-Pliszczynska, J.
Powiązania:
https://bibliotekanauki.pl/articles/363100.pdf
Data publikacji:
2009
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
test biologiczny
ryba
PCDD/Fs
metody przesiewowe
bioassay
fish
screening methods
Opis:
Among 210 congeners only 17 highly toxic, 2,3,7,8-chlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) are of toxicological concern. They exhibit high binding affinity to an intracellular aryl hydrocarbon receptor (AhR), causing harmful effects at exposure levels of thousands of times lower than most environmental toxicants. The Chemically Activated LUciferase gene expression bioassay (CALUX) utilizes recombinant cells that were transfected with a luciferase reporter gene, which responds to dioxin-like compounds with the induction of luciferase in a time-, dose-, AhR-dependent and chemical-specific manner. The bioassay evaluation concerning the European Community (EC) requirements for the PCDD/Fs determination for the official control of foodstuffs was performed on salmon tissue. In order to evaluate the bioassay performance characteristics, recovery range, limit of detection (LOD), limit of quantification (LOQ) and precision were determined. The results revealed that combining a dichloromethane: hexane extraction, an acid silica plus activated carbon clean-up provides reliable, reproducible (CV = 9-20%) measurements with acceptable recovery (78%) and sensitivity at the required ppt range. Due to the low cost and high throughput characteristics of the CALUX assay, food monitoring for PCDD/Fs may benefit from use of this bioassay as a prescreening tool to select and prioritize samples for subsequent analysis by high resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS). Although the bioassay may not be able to specify identity of the reactive substances, it may serve as a very useful tool for the evaluation of contamination sources.
Źródło:
Environmental Biotechnology; 2009, 5, 1; 32-41
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Behavioral and Lethal Effects of La Salt and a Mixture of Cu and La Salt on Daphnia magna Straus
Autorzy:
Olkova, Anna Sergeewna
Sysolyatina, Maria
Powiązania:
https://bibliotekanauki.pl/articles/2105282.pdf
Data publikacji:
2022
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
lanthanum
copper
bioassay
Daphnia magna
motor activity
joint effect
synergism
potentiation
Opis:
The study of joint effects of REE and HM is relevant, since they are often satellite deposits, their areas of application are similar, and an increase in concentrations of elements of these groups in the areas that are not places of their extraction and enrichment is proven. The purpose of this work was to find out the pre-lethal and lethal effects of La, Cu and their equimolar mixtures in tests for Daphnia magna Straus. Bioassays of artificially polluted natural waters, initially free of toxic elements, was carried out. In bioassays on the mortality of D. magna in the space of 96 hours it was found that acute toxicity of copper sulfate solutions is observed at the calculated concentration of Cu2+ 0.1 mg/L (0.0016 mmol/L), and the acute toxicity of lanthanum sulfate is when the dose of La3+ is equal to 50 mg/L (0.36 mmol/L). In the solutions comprising mixtures of Cu and La salts (1:1 calculated using metals), the concentrations of which are equimolar to the investigated solutions of copper sulfate, the mortality of D. magna begins in the solution containing 10 times less toxic elements. It was found that 25% of individuals died in the variant “0.00016 mmol/L”, the mortality of 100% of individuals was at the total metal concentration of 0.0008 mmol/L. The solutions containing La (0.072–0.72 mmol/L) and Cu (0.00016–0.0016 mmol/L) naturally inhibit the motor activity of D. magna by 1.3–5.3 times and 1.2–1.9 times in 1 hour and 1.7–2.8 and 1.4–2.2 times in 24 hours, respectively. The solutions containing mixtures of Cu and La salts inhibited the motor activity of D. magna in the same way as copper sulfate solutions with the Cu2+ concentrations equimolar “Cu2+ + La3+”. Therefore, when testing the solutions with the same molar concentrations of Cu2+ and the mixture of “Cu2+ + La3+” it was shown that La potentiates the pre-lethal effect of Cu to the level of individual effects of Cu. The additions of La salt to the solutions containing pre-lethal doses of Cu lead to lethal effects of such mixtures for D. magna.
Źródło:
Journal of Ecological Engineering; 2022, 23, 6; 245--252
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Use of the Triad Approach to Ecological Assessment of the State of a Reservoir Receiving Wastewater From a Thermal Power Plant
Autorzy:
Mahanova, Elena
Powiązania:
https://bibliotekanauki.pl/articles/1839092.pdf
Data publikacji:
2021
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
thermal power plant
chemical analysis of water
bioassay
bioindication
pollution
trophicity
Opis:
Thermal power plants (TPP) belong to the enterprises that accompany the development of any city. These industrial facilities consume and discharge large amounts of water. Therefore, thermal power plants are often located near water bodies that need to assess their ecological state and predict the consequences of the anthropogenic impact. The purpose of the work was to assess the ecological state of the floodplain Lake Ivanovskoye (Russia, Kirov region), which receives the wastewater from a thermal power plant, using a triadic approach and comparing the results of chemical analysis, bioassay and bioindication. It was shown that the main pollutant in the lake and the canal connecting Lake Ivanovskoye with the nearest river Vyatka is ammonium ion (up to 3.2 mg/dm3). In addition, the Russian standards for biological oxygen consumption are exceeded (analysis period is 20 days). The tests for the toxicity of wastewater showed inhibition of Paramecium caudatum and Escherichia coli reactions. In contrast, natural surface water from Lake Ivanovskoye stimulated the test functions of Scenedesmus quadricauda, P. caudatum, and E. coli. The death of Daphnia magna in the samples were not recorded. The totality of the facts pointed to the organic nature of pollution, leading to the anthropogenic eutrophication of the lake. The hypothesis was tested by using the bioindication method and by analyzing the totality of species of coastal aquatic plants. It was shown that the reservoir belongs to the mesotrophic type with a moderate degree of pollution, which decreases with distance from the wastewater discharge points. The increase in the trophicity of the reservoir is facilitated by thermal pollution (up to +15℃) and an alkaline pH of wastewater (up to 9.2). Thus, the joint analysis of the results of chemical analysis, bioassay and bioindication made it possible to carry out an objective assessment of the ecological state of the reservoir and identify the reasons for the revealed facts.
Źródło:
Journal of Ecological Engineering; 2021, 22, 2; 155-160
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis, characterization and evaluation of mosquito larvicidal effect of 1,3,4-oxadiazole derivatives against Culex quinquefasciatus
Autorzy:
Santhanalakshmi, K.
Kalyanasundharam, S.
Muthukumar, S.
Rosy, P. Jacquline
Powiązania:
https://bibliotekanauki.pl/articles/1112036.pdf
Data publikacji:
2018
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
1
3
4-oxadiazole
Culex quinquefasciatus
Larvicidal bioassay
ultrasonic irradiation
Opis:
A new series of 1,3,4-oxadiazole derivatives were prepared from the condensation of acid hydrazide,5-bromo-2-(trifluoromethoxy)benzoic acid in POCl3 by ultrasonic irradiation method. The new synthetic method furnished the desired products in shorter time and good yields. The chemical structures of compounds 4a-h were confirmed by Fourier transform infrared spectroscopy (IR), proton nuclear magnetic resonance (1H-NMR), carbon nuclear magnetic resonance (13C-NMR) and elemental analyses. Larvicidal bioassay tests were performed using several 2-(5-bromo-2-(trifluoromethoxy) phenyl)-5-aryl-1,3,4-oxadiazole. These synthesized compounds presented strong larvicidal activity against urban mosquitoes Culex quinquefasciatus. The results suggest that larvicidal activity might be correlated with the presence of electron-withdrawing substituents in the para position of the phenyl ring. The alterations observed in the larvae spiracular valves of the siphon and anal papillae by 1,3,4-oxadiazoles in the larvicidal bioassay are responsible for larvae’s death.
Źródło:
World News of Natural Sciences; 2018, 19; 58-64
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Allelopathy in Solanaceae plants
Autorzy:
Mushtaq, W.
Siddiqui, M.B.
Powiązania:
https://bibliotekanauki.pl/articles/65653.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
plant growth
allelopathy
allelochemical
bioassay
plant extract
fraction
residue
Solanaceae
Opis:
Solanaceae plants have strong allelopathic potential, and therefore the action is confirmed through: a) bioassays with liquid or various solvent extracts and residues, b) fractionation, identification, and quantification of causative allelochemicals. Most assessments of allelopathy involve bioassays of plant or soil extracts, leachates, fractions, and residues which support seed germination and seedling growth in laboratory and greenhouse experiments. Plant growth is also stimulated below the allelopathic threshold, however severe growth reductions may be observed above the threshold concentration depending on the sensitivity of the receiving species. Generally, seedling growth is more sensitive than germination, particularly root growth. Some approaches showed that field soil collected beneath donor plants significantly reduced or somewhat promoted the growth of the recipients plants. Petri dish bioassays with aqueous extracts of different parts of donor plants showed considerable phytotoxic activities in a concentration-dependent manner with leaf aqueous extracts being most dominant. Delayed seed germination and slow root growth attributable to the extracts may be baffled with diffusion effects on the rate of imbibition, delayed initiation of germination, and particularly cell elongation; the main factor that is responsible for affecting root growth before and after the tip penetrates the testa. Light and electron microscopy extract analysis at the ultrastructural level are correctly investigated. Several Solanaceae plants have allelopathic potential, and therefore the activities, kinds and quantity of allelopathic compounds differ depending on the plant species. The incorporation of allelopathic substances into agricultural management might scale back the development of pesticides and reduce environmental deterioration.
Źródło:
Journal of Plant Protection Research; 2018, 58, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Toksyczność chlorku 1-heksylo-3-metyloimidazoliowego względem wybranych organizmów wodnych
Toxicity of 1-heksyl-3-methylimidazolium chloride according to selected marine and freshwater organisms
Autorzy:
Arendarczyk, A.
Zgórska, A.
Grabińska-Sota, E.
Powiązania:
https://bibliotekanauki.pl/articles/296931.pdf
Data publikacji:
2011
Wydawca:
Politechnika Częstochowska. Wydawnictwo Politechniki Częstochowskiej
Tematy:
imidazoliowe ciecze jonowe
toksyczność
biotest
imidazolium
ionic liquids
toxicity
bioassay
Opis:
Dokonano oceny potencjału toksykologicznego imidazoliowej cieczy jonowej o nazwie chlorek 1-heksylo-3-metyloimidazoliowy (CHM). Zakres badań ekotoksykologicznych obejmował przeprowadzenie testu toksyczności ostrej z wykorzystaniem skorupiaków słodkowodnych (Thamnocephalus platyurus) i testu toksyczności chronicznej z wykorzystaniem bakterii morskich (Vibrio fischeri). Wartości wskaźników ekotoksykologicznych wyznaczone w poszczególnych testach wyniosły odpowiednio: EC50 2,51 mg/dm3 (THAMNOTOXKIT FTM) i EC50/5min 15,30 mg/dm3 i EC50/15min 14,40 mg/dm3 (MICROTOX® Basic Test 81,9%). Na podstawie uzyskanych wyników związek sklasyfikowano jako toksyczny. W ramach badań dokonano również oceny wpływu korekty odczynu i napowietrzania na redukcję toksyczności roztworu CHM. Doświadczenie przeprowadzono zgodnie z metodyką US EPA/600/6-91/005F. Stwierdzono, że korekta odczynu roztworu CHM do poziomu 3 i 11, a także przeprowadzenie symultanicznego procesu korekty pH do 3 i napowietrzania wpływa na obniżenie toksyczności badanego związku.
Ionic liquids (IL's) are defined as organic salts with low melting point. The reputation of those substances as green solvents (environmental friendly chemicals) is based primarily on their negligible vapor pressure. However, high solubility and stability of those substances in water can cause a serious concern about their toxic potential. Relatively little is still known about the toxicity of these substances as a class, especially when compared to conventional organic solvents. The aim of the study was to investigate the toxicity of imidazolium ionic liquid: 1-heksyl-3-methylimidazolium chloride (CHM) according to selected aquatic organisms: bioluminescent marine bacterium and crustacean. The results showed that CHM caused a harmful effect on tested organisms in the whole range of concentrations used in the tests. The estimated toxic index EC50 obtained 2.51 mg/dm3 according to Thamnocephalus platyurus and EC50/5min 15.30 mg/dm3, EC50/15min 14.40 mg/dm3 according to Vibrio fischeri. The CHM was classified as a toxic substance. Moreover, the toxicity of CHM samples with modified pH values and aeration has been measured. Modifications of CHM samples were based on U.S. EPA Protocol (Toxicity Identification Evaluation). Acute toxicity of each modification have been determined by using the MICROTOX® Basic Test 81.9% standard procedure. The results showed that upgrading pH value to 11 as well as diminishing samples pH to value 3 had an influence on samples' post-reaction toxicity. Both processes, pH correction and aeration, used individually caused a significant decrease of analyzed samples' toxicity expressed as toxic index's EC50 measured after 5 and 15 minutes of exposition. The toxic index values were increased from range 30% (pH 3) up to 100% (pH 11) according to non modified sample. The study showed that pH correction to value 3 connected with simultaneous aeration additionally decrease sample toxicity. The results of the present study has proved that 1-heksyl-3methylimidazolium chloride could be toxic to aquatic organisms and it is necessary to carry out the ecotoxicological risk of ionic liquids by the use of bioassays.
Źródło:
Inżynieria i Ochrona Środowiska; 2011, 14, 2; 137-143
1505-3695
2391-7253
Pojawia się w:
Inżynieria i Ochrona Środowiska
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Intraspecific Sensitivity to Toxicants – a Methodological Problem of Bioassay: Review
Autorzy:
Olkova, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1955380.pdf
Data publikacji:
2021
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
bioassay
environmental pollution
intraspecific sensitivity
genetic factors
genetically unconditioned factors
Opis:
The article discusses the scientific studies that reveal the mechanisms of intraspecific differences in responses of organisms to chemical exposure. The factors of individual sensitivity to toxicants are represented by genetic differences between individuals of the same species and genetically unconditioned factors. The genetic factors are mutations, including under-researched mitochondrial DNA mutations and genomic drift, genetic polymorphism, and gender-related differences. Age, conditions of keeping and state of organisms, seasonal variations of body functions, and biotic interactions are considered as the factors that are not related to the genetic apparatus. The article considers a number of works, in which the effects of the combined action of external factors on sensitivity of organisms in model groups are studied.
Źródło:
Journal of Ecological Engineering; 2021, 22, 7; 113-122
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biotest as an indicator of dioxin-like PCBs presence
Zastosowanie biotestu jako indykatora obecności dioksynopodobnych PCB
Autorzy:
Małagocki, P.
Piskorska-Pliszczyńska, J.
Powiązania:
https://bibliotekanauki.pl/articles/125760.pdf
Data publikacji:
2010
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
dioxins
dioxin-like PCB
bioassay
detection methods
dioksyny
dioksynopodobne PCB
biotest
metoda detekcji
Opis:
Dioxins (polychlorinated dibenzo-p-dioxins, PCDDs, polychlorinated dibenzofurans, PCDFs) and dioxin-like polychlorinated biphenyls (dl-PCBs) are toxic compounds which are commonly present in the food chain. According to the European Union regulations the presence of these compounds in food may be determined by complementary and screening and methods considered confirmatory. The combination of the two methods allows a relatively fast selection of food and feed samples containing dioxins at concentrations exceeding acceptable levels and permits an unequivocal confirmation of tested compounds. The aim of the study was to evaluate the usefulness of the biotest based on genetically modified cell lines sensitive to PCDDs and PCDFs for the simultaneous detection and quantitative determination of dl-PCBs in food samples. Mouse hepatoma cell lines (Hepa1L6.1c3) with the luciferase reporter gene were used. The principle of the biotest involving the dioxin mechanism of action includes the presence of receptor Ah agonist Hepa1L6.1c3 cell line synthesize luciferase at concentrations proportional to the agonist dose. The measurement of luciferase activity for various 2,3,7,8-TCDD concentrations (calibration curve) permits quantitative measurements of the Ah receptor antagonist content in examined food samples. The extraction of dl-PCBs from matrixes (salmon, herring and sprat muscles) was performed using the extraction and purification procedure for PCDDs/PCDFs analysis. Separation of dl-PCB from PCDD/PCDF was done by a column chromatography. The concentration of dl-PCBs was determined using the biotest. The results involving the same samples were compared with those obtained by the HRGC/HRMS method regarded as confirmatory. The results confirm the biotest as a useful method for dl-PCBs determinations; the results obtained by the two methods are parallel and meets the criteria defined by the Commission Regulation (EC) No. 1881/2006.
Dioksyny (polichlorowane dibenzo-p-doksyny (PCDD) i polichlorowane dibenzofurany (PCDF)) oraz dioksynopodobne polichlorowane bifenyle (dl-PCB)) są związkami toksycznymi występującymi m.in. w łańcuchu żywieniowym. Zgodnie z przepisami Unii Europejskiej, do oznaczania ich zawartości w żywności można zastosować metody komplementarne: przesiewową i potwierdzającą. Ich połączenie pozwala na stosunkowo szybką selekcję próbek żywności i pasz zawierających dioksyny w stężeniach przekraczających dopuszczalne poziomy oraz pozwala na jednoznaczne potwierdzenie obecności badanych związków. Celem pracy było określenie przydatności biotestu bazującego na genetycznie zmodyfikowanej linii komórek wrażliwych na działanie PCDD i PCDF do równoczesnego wykrywania i ilościowego oznaczania dl-PCB w próbkach żywności. W badaniach zastosowano linię komórkową hepatomy mysiej (Hepa1L6.1c3) z wprowadzonym transgenem lucyferazy pod kontrolą receptora Ah. Zasada biotestu wykorzystującego mechanizm działania dioksyn jest następująca: w obecności agonistów receptora Ah komórki Hepa1L6.1c3 syntezują enzym lucyferazę w stężeniu proporcjonalnym do dawki agonisty. Pomiar aktywności lucyferazy wobec serii stężeń 2,3,7,8-TCDD (krzywa kalibracyjna) pozwala na ilościową ocenę zawartości agonistów receptora Ah w badanym ekstrakcie próbki żywności. Do ekstrakcji dl-PCB z matrycy (mięśnie łososia, śledzia, szprota) użyto zoptymalizowanej dla PCDD/PCDF metody ekstrakcji i oczyszczania. Na kolumnach chromatograficznych oddzielano dl-PCB od PCDD/PCDF. Zawartość dl-PCB oznaczano, stosując biotest. Wyniki uzyskane biotestem porównywano z rezultatami oznaczeń chemiczną metodą potwierdzającą HRGC/HRMS, wykonaną w tych samych próbkach. Uzyskane dane pozwalają ocenić biotest jako przydatne narzędzie w badaniach zawartości dl-PCB, ponieważ wyniki uzyskane obydwoma metodami są porównywalne, a metoda spełnia kryteria określone przepisami prawa wspólnotowego (Rozp. 1881/2006/WE).
Źródło:
Proceedings of ECOpole; 2010, 4, 2; 267-272
1898-617X
2084-4557
Pojawia się w:
Proceedings of ECOpole
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of Sensitivity of Autotrophic and Heterotrophic Microorganisms to the Pollution of Natural Water with Rare Earth Elements (Lanthanum and Cerium)
Autorzy:
Lozhkina, Roza A.
Olkova, Anna S.
Koval, Ekaterina V.
Tomilina, Irina I.
Sysolyatina, Maria A.
Powiązania:
https://bibliotekanauki.pl/articles/2086379.pdf
Data publikacji:
2022
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
rare earth elements
lanthanum
cerium
bioassay
Nostoc linckia
Chlorella vulgaris
Paramecium caudatum
Escherichia coli
Opis:
The purpose of the work was to compare the sensitivity of autotrophic and heterotrophic organisms used in bioassay to lanthanum and cerium under the conditions of a model experiment with aqueous media. Using bioassay methods, the pre-lethal effects of La and Ce in heterotrophic Paramecium caudatum and Escherichia coli, as well as autotrophic Chlorella vulgaris and Nostoc linckia were determined. Model solutions of La2 (SO4)3∙8H2O and Ce2 (SO4)3∙8H2O were tested in the concentration range of 0.1–200 mg/l. As a result, it was shown that heterotrophic organisms are more sensitive to water pollution with La and Ce than autotrophic ones. According to the totality of experiments, cerium turned out to be more toxic than lanthanum. When planning the environmental studies of wastewater or reservoirs polluted with REE, it is recommended to focus on comparative sensitivity of bioassay methods, taking into account the test-functions used: bioassay for chemotaxis of P. caudatum > bioassay for changes in bioluminescence of E. coli (strain M-17) > bioassay for the content of chlorophyll а and intensity of lipid peroxidation in N. linckia > bioassay on the increase in the number of Cl. vulgaris.
Źródło:
Journal of Ecological Engineering; 2022, 23, 4; 58--63
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Amphipods in estuarine and marine quality assessment – a review
Autorzy:
Podlesinska, W.
Dabrowska, H.
Powiązania:
https://bibliotekanauki.pl/articles/48648.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
amphipod
marine sediment
aquatic ecosystem
ecological quality
estuary
bioassay
abiotic factor
biological factor
Źródło:
Oceanologia; 2019, 61, 2
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A comparison of phytotoxic potential among the crude extracts from Parthenium hysterophorus L. extracted with solvents of increasing polarity
Autorzy:
Pati, U.K.
Chowdhury, A.
Powiązania:
https://bibliotekanauki.pl/articles/11232.pdf
Data publikacji:
2015
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
comparison
phytotoxicity
Vigna radiata
crude extract
Parthenium hysterophorus
bioassay
hexane
ethyl acetate
methanol
solvent
Opis:
There is a worldwide search for the safe, effective and eco-friendly compounds of plant origin to combat the weed species and other pests which are responsible for the great impact on the growth and productivity of agricultural crops. In this present study, a comparison was made to evaluate the phytotoxicity potential of sequentially extracted solvent (hexane, ethyl-acetate, methanol) extracts of Parthenium hysterophorus L. (aerial parts) in vitro through bench-top seed germination assay (Vigna radiata L.). One-way analysis of variance (ANOVA) followed by Duncan’s multiple range test (DMRT) were done for statistical analysis of the data. The study reveals that germination, growth and vigour was significantly (P<0.05) reduced by ethyl-acetate and methanol extracts. The present study concluded that phytotoxicity of ethylacetate and methanolic crude extracts of Parthenium hysterophorus could be exploited as potential bioherbicide for future weed management programme and the development of bioherbicide for commercial use.
Źródło:
International Letters of Natural Sciences; 2015, 06
2300-9675
Pojawia się w:
International Letters of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
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