Temat: anther culture - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Wheat anther culture as affected by various cultural changes and supplements
Autorzy:: Simonson, R L
Baenziger, P S
Gustafson, V D
Powiązania:: https://bibliotekanauki.pl/articles/2046627.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: cultural change
supplement
wheat
silver nitrate
Triticum aestivum
anther culture
cereal tissue
Opis:: With the numerous improvements in cereal tissue and wheat anther culture, it is necessary to determine which of the improvements should be combined for optimal response. This study was conducted using one highly responsive cultivar of wheat (Triticum aestivum L. cv. Pavon 76) to test the effectiveness of pre-culture cold treatment (0 or 3-7 days at 5°C) of anthers, five initiation basal media, and various changes in Murashige-Skoog regeneration media. A cold pre-culture treatment was inhibitory for all initiation media for embryoid initiation. Of the initiation media, P1, 85D12, and N6 were similar for embryoid initiation (0.80 to 0.90 embryoids/anther) without a cold pre-culture treatment. Plant regeneration was improved by the addition of amino acids or glucose, increased sucrose concentration, filter sterilizing the medium, and altering plant growth regulator concentrations. P1 medium which is normally used for embryoid initiation was also beneficial for improving plant regeneration. Ethylene inhibitors were generally not beneficial.
Źródło:: Journal of Applied Genetics; 1997, 38, 4; 381-392
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: The androgenic response in anther culture of spring wheat is greater in genotypes with solid stem in contrast to genotypes with hollow stem
Autorzy:: Weigt, D.
Kiel, A.
Nawracala, J.
Pluta, M.
Powiązania:: https://bibliotekanauki.pl/articles/951280.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: anther culture
spring wheat
stem
genotype
water-soluble carbohydrate
androgenesis
breeding programme
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Somaclonal selection of physiological mutants and several problems related to rice cell breeding
Autorzy:: Kinoshita, T
Mori, K.
Powiązania:: https://bibliotekanauki.pl/articles/2044460.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: chromosome variation
japonica rice
Oryza
African rice
physiological mutant
gamma-radiation
anther culture
hybrid plant
protoplast isolation
callus induction
callus formation
aneuploidy
indica rice
herbicide tolerance
hybrid
Kitaake cultivar
gametoclonal variation
somatic hybrid
rice
Opis:: Tolerance to a new herbicide, pyributycarb, was evaluated both at the plant and cellular levels. Several highly or moderately tolerant strains chosen at the plant level, showed a parallel relation of to tolerance at the cellular level. However, on the whole, correlation between total tolerance indices and survival rates of calli was not significant in 18 out of the 80 studied strains. As a result of somaclonal selection for two herbicides, lines NB-200 and NM-100 were regenerated from the tolerant calli screened with benthiocarb at 200 ppm, and molinate at 100 ppm, respectively. In the R₂ generation, both the lines displaned a stable tolerance both at the plant and cellular levels. Thus the highly tolerant mutant lines were developed from a moderately tolerant line, N-61, via in vitro selection. To achieve a short-cut method in the interspecific genetic exchange, a series of techniques related to cell fusion were established in rice and related species. Two kinds of somatic hybrids between the cultivar Kitaake and tetraploid Oryza species, O. punctata and O. officinalis, were successfully produced. Among the somatic hybrid plants, a wide range of chromosomal variation was observed. Aneuploid plants with a chromosome number around 2n = 72 (hexaploid), which are expected from a symmetric fusion between diploid and tetraploid strains, were obtained showing mixoploidy within a plant. Most of the somatic hybrids were characterized by intermediate features of plant-type showing high sterility, shattering of spikelets and reduced plant height. As an exception, a diploid plant, which was identified by RFLP analysis using the rDNA gene probe, closely resembled Kitaake and produced viable seeds. A tetraploid hybrid plant was also promising for the introduction of economically important characters through the reduction of chromosome numbers by doubled haploids. Gametoclonal variation and gamma radiation was applied to Kitaake. The mutation frequency was prominently increased by gamma ray treatment, especially at high doses of 200 Gy or 300 Gy. In the M₃R₂ or M₄R₃ generations, most of the variants showed unfavourable characters. Most of the mutant characters were governed by single or double recessive genes. Several mutants such as short culm and early flowering time might be used for rice breeding.
Źródło:: Journal of Applied Genetics; 1998, 39, 1; 9-35
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Rice improvement, involving altered flower structure more suitable to cross-pollination, using in vitro culture in combination with mutagenesis
Autorzy:: Min, S K
Powiązania:: https://bibliotekanauki.pl/articles/2048292.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: japonica rice
radiation
China
mutagenesis
mutant
anther culture
in vitro
cross-pollination
hybridization
indica rice
somatic cell
Oryza sativa
rice
Opis:: Anther and somatic tissue culture in combination with mutagenesis were carried out to evaluate the efficiency of different mutagenic treatments of various in vitro culture materials, and to obtain some promising variants for rice improvement. Results indicated that in japonica rice radiation treatment of dry seeds and young panicles influenced the percentage of green plantlets regeneration from anther culture. Both treatments increased significantly the percentage of regenerated green plantlets in comparison with the control. Irradiation with 30 Gy of rice callus increased also the percentage of regenerated green plantlets. For indica rice, the combination of the suitable dose of gamma rays irradiation on seeds and an improved medium, increased the percentage of callus induction. This approach made it possible to use anther culture in indica rice breeding. Somatic tissue cultures combined with radiation-induced mutagenesis led to the development of a number of promising mutants including some new cytoplasm-nucleus interacting male-sterile lines with almost 100% stigma exsertion. Their development would be of practical significance for increasing the genetic diversity for production of hybrid rice.
Źródło:: Journal of Applied Genetics; 1998, 39, 2; 151-162
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Regeneration of oat androgenic plants in relation to induction media and culture conditions of embryo-like structures
Autorzy:: Ponitka, A
Slusarkiewicz-Jarzina, A.
Powiązania:: https://bibliotekanauki.pl/articles/57988.pdf
Data publikacji:: 2009
Wydawca:: Polskie Towarzystwo Botaniczne
Tematy:: oat
Avena sativa
hybrid
plant regeneration
haploid plant
doubled haploid
anther culture
embryo-like structure
culture condition
Opis:: The effect of C17 and W14 induction media on the formation of embryo-like structures (ELS) from F3 generation of nine hexaploid oat hybrids was investigated in the study. In all genotypes, the highest number of ELS (0.6 - 12.1/100 anthers) was obtained on C17 medium. The efficiency of plant regeneration on medium 190-2 was tested, in relation to different ELS culture conditions. The highest rate of green plants per 100 ELS (3.3 - 42.4) was produced by incubation at 22oC in the dark for the first two weeks. Among 36 green regenerants, 28 (77.8%) were haploid and 8 (22.2%) were spontaneous doubled haploids, fully fertile. After colchicine treatment of haploid plants, 19 were partially fertile and set from 1 to 15 seed per panicle.
Źródło:: Acta Societatis Botanicorum Poloniae; 2009, 78, 3; 209-213
0001-6977
2083-9480
Pojawia się w:: Acta Societatis Botanicorum Poloniae
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Production and evaluation of doubled haploid wheat lines
Autorzy:: Pauk, J
Kertesz, Z
Powiązania:: https://bibliotekanauki.pl/articles/2046625.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: wheat line
production
doubled haploid
cross combination
haploid plant
anther response
Triticum aestivum
anther culture
F3 wheat line
F1 population
F4 wheat line
F2 population
combining ability
Opis:: F₁, F₂ populations as well as F₃ and F₄ wheat lines coming from more than 300 cross combinations have been used for androgenic wheat studies and more than 5000 haploid plants were produced. Promising doubled haploid lines were tested together with lines produced in the conventional way in yield trials. The two best doubled haploid lines overyielded the standard. On the basis of results related to the general combining ability it is advisible to cross parents with a good combining ability for characters such as high percentage of responding anthers and embryo/callus induction with parents indicating a good combining ability for green plant production. In this way ideal lines can be obtained for doubled haploid production.
Źródło:: Journal of Applied Genetics; 1997, 38, 4; 425-435
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Otrzymywanie spontanicznych i indukowanych linii podwojonych haploidów pszenżyta ozimego z wykorzystaniem kultur pylnikowych
Production of spontaneous and induced doubled-haploid lines of winter triticale obtained through anther culture
Autorzy:: Ponitka, Aleksandra
Ślusarkiewicz-Jarzina, Aurelia
Powiązania:: https://bibliotekanauki.pl/articles/2198115.pdf
Data publikacji:: 2011-09-30
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: haploid
kultury pylnikowe
linie DH
pszenżyto ozime
anther culture
DH lines
winter triticale
Opis:: W kulturach pylnikowych 15 form mieszańcowych pszenżyta ozimego badano częstotliwość uzyskiwania androgenicznych roślin. Ze wszystkich genotypów otrzymano zielone rośliny, przy czym częstotliwość wahała się od 0,4 do 15,2/100 pylników w poszczególnych genotypach. Dla regeneracji roślin zastosowano pożywkę 190-2 z dwoma stężeniami kinetyny (0,5 lub 1,5 mg/l). Na pożywce zawierającej 0,5 mg/l kinetyny otrzymano więcej zielonych roślin, tj. od 2,5 do 46,7/100 androgenicznych struktur. Poziom ploidalności oznaczono cytometrycznie u 350 roślin i w zależności od genotypu stwierdzono od 17,6 do 80,0% spontanicznie podwojonych haploidów. Ogółem uzyskano 80,3% linii DH pszenżyta ozimego, wśród których było 38,0% spontanicznie podwojonych haploidów, natomiast w wyniku kolchicynowania 209 roślin haploidalnych otrzymano 70,8% linii DH.
We investigated the rate of obtaining androgenic plants using anther culture of 15 winter triticale hybrids. Green plants were obtained from all genotypes, with the frequency ranging from 0.4 to 15.2/100 anthers. The medium 190-2, with two different concentrations of kinetin (0.5 and 1.5 mg/l), was used for plant regeneration. Higher number of green plants was observed in the case of the medium with 0.5 mg/l of kinetin (2.5–46.7/ 100 androgenic structures). Ploidy level was determined by flow cytometry in 350 plants and showed from 17.6 to 80.0% spontaneously doubled haploids, depending on the genotype. A total of 80.3% of DH lines was obtained, among which 38.0% were spontaneously doubled haploids, whereas 70.8% of DH lines was obtained from colchicine treatment of 209 haploid plants.
Źródło:: Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2011, 260/261; 183-191
0373-7837
2657-8913
Pojawia się w:: Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Otrzymywanie podwojonych haploidów z mieszańców ozimych i jarych pszenżyta z zastosowaniem kolchicyny w kulturach pylnikowych
Doubled haploid production of winter and spring triticale hybrids using colchicine in anther cultures
Autorzy:: Ślusarkiewicz-Jarzina, Aurelia
Ponitka, Aleksandra
Powiązania:: https://bibliotekanauki.pl/articles/2198759.pdf
Data publikacji:: 2015-06-30
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: kolchicyna
kultury pylnikowe
podwojone haploidy
pszenżyto ozime
pszenżyto jare
colchicine
anther culture
doubled haploids
winter triticale
spring triticale
Opis:: Celem pracy było podwyższenie efektywności otrzymywania podwojonych haploidów w kultu¬rach pylnikowych 6 form mieszańcowych pszenżyta ozimego i 6 form jarych poprzez zastosowanie kolchicyny w pożywce indukującej androgenezę. Modyfikowano stężenie kolchicyny i czas traktowania (0,1 mg/l lub 1,0 mg/l kolchicyny odpowiednio przez 24, 48 i 72 godziny) w płynnej pożywce C17. Stwierdzono, że optymalnymi parametrami zastosowania kolchicyny były stężenia 1,0 mg/l przez 24 godziny oraz 0,1 mg/l przez 48 godzin. Na podstawie analiz cytometrycznych, w tych warunkach stwierdzono zwiększenie liczby roślin o podwojonej liczbie chromosomów. Wynik ten obserwowano u 6 form ozimych (średnio 66,2%) oraz u 2 form jarych (średnio 52,7%), w porów¬naniu z warunkami kontrolnymi na pożywce bez kolchicyny, w których uzyskano około 30% podwojonych haploidów.
The aim of this work was to increase the efficiency of doubled haploids production in anther cultures of 6 winter and 6 spring triticale hybrids. The colchicine concentration and the time of its use (0.1 mg/l or 1.0 mg/l colchicine, for 24, 48 and 72 h, respectively) in androgenesis inducing C17 liquid medium were modified. It was found that the optimal parameters of colchicine treatment were 1.0 mg/l for 24 hours, and 0.1 mg/l for 48 hours. Based on flow cytometry analysis, in those conditions an increase in a number of doubled haploids was observed. The increase was observed in 6 forms of winter (average 66.2%) and 2 forms of spring triticale (average of 52.7%), as compared to control conditions (the C17 medium without colchicine), producing of about 30.0% of doubled haploids
Źródło:: Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2015, 276; 57-67
0373-7837
2657-8913
Pojawia się w:: Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Ocena zdolnosci pylnikow wybranych odmian lnu oleistego [Linum usitatissimum L.] do tworzenia kalusa
Estimation of the ability of anthers from different linseed cultivars [Linum usitatissimum L.] for callus induction
Autorzy:: Wielgus, K
Mankowska, G.
Powiązania:: https://bibliotekanauki.pl/articles/832665.pdf
Data publikacji:: 2006
Wydawca:: Instytut Hodowli i Aklimatyzacji Roślin
Tematy:: rosliny oleiste
len oleisty
Linum usitatissimum
odmiany roslin
pozywki hodowlane
kultury in vitro
kultury pylnikowe
kalus
oil plant
linseed
plant cultivar
culture medium
in vitro culture
anther culture
callus
Źródło:: Rośliny Oleiste - Oilseed Crops; 2006, 27, 2; 213-222
1233-8273
Pojawia się w:: Rośliny Oleiste - Oilseed Crops
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Influence of genetic and environmental factors on anther culture response of wheat
Autorzy:: Zhou, H
Konzak, C F
Powiązania:: https://bibliotekanauki.pl/articles/2046623.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: callus response
plant regeneration
environmental factor
doubled haploid
wheat
genetic factor
Triticum aestivum
anther culture
Opis:: The influences of genetic and environmental factors on the anther culture responses of wheat were investigated. Significant differences for callus induction, plant regeneration, and green plant percentages were observed when the nucleus of Triticum acstivum L. cv. Selkirk was transferred to ten alien cytoplasms by substitution backcrosses. In most cases, the alien cytoplasms decreased anther culture responses, but sometimes they were as good as or better than the T. aestivum cytoplasm. Significant within-genotype variation for anther culture responses were observed for wheat varieties Chris, Yecora Rojo, WA7176 and Edwall, indicating genetic heterogeneity in the present commercial cultivars, and potential for improving anther culture responses by in vitro prescreening. When five genotypes (Chris, Pavon 76, Butte 86, WA6916, and Edwall) were cultured across three (potato-4 liquid, 100 g L⁻¹ ficoll-supplemented, and 6 g L⁻¹ agar-solidified) induction media, the liquid and ficoll-containing media were 10 to 15 times more productive than the agar-solidified medium. Whereas, the ficoll medium was not significantly different from the liquid medium. Several low concentration starch media appeared promising to replace current induction media. The starch media sustained the highcallus-induction properties of the liquid medium, while improving callus aeration similar to that observed on solid media, resulting in markedly higher plant regeneration and green plant percentages.
Źródło:: Journal of Applied Genetics; 1997, 38, 4; 393-406
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Induction of multi-nucleate microspores in anther culture of salix viminalis L.
Autorzy:: Wojciechowicz, M K
Kikowska, M.A.
Powiązania:: https://bibliotekanauki.pl/articles/41619.pdf
Data publikacji:: 2009
Wydawca:: Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:: Salix viminalis
genotype
androgenesis
thermal factor
medium composition
stress response
thermal shock
anther culture
multinucleate microspore
male clone
mannitol
Kyo medium
microspore
ultrastructural analysis
initial microspore
Opis:: The aim of the work was to determine how the induction of androgenesis in selected genotypes of Salix viminalis is affected by thermal factors and medium composition. Anthers isolated from male clones of S. viminalis were pre-cultured at 4, 27 and 32°C for two to eight days in liquid Kyo medium with and without the addition of mannitol and on solid MS or WPM medium. The solid media were supplemented with different concentration of disaccharidesand various combinations of growth regulators including kinetin, 2iP, IAA and IBA. Multi-nucleate microspores indicative of androgenesis were observed in anthers pre-cultured for seven days at 4°C in liquid Kyo medium containing mannitol. The rate of androgenesis was higher when the anthers were transferred to solid modified MS medium containing high concentrations of sucrose and kinetin. In studied genotypes of basket willow early uninucleate microspores underwent sporophytic divisions. Ultrastructural observations showed differences in cellular arrangement of pre-stressed microspores. In Salix viminalis, microspores without starch grains and decreased number of lipid bodies were potentially androgenic cells.
Źródło:: Dendrobiology; 2009, 61; 55-64
1641-1307
Pojawia się w:: Dendrobiology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Induced androgenesis in Phleum pratense
Indukowana androgeneza w Phleum pratense
Autorzy:: Broda, Z.
Mikołajczyk, S.
Powiązania:: https://bibliotekanauki.pl/articles/2234853.pdf
Data publikacji:: 2006
Wydawca:: Polskie Towarzystwo Łąkarskie
Tematy:: induced androgenesis
androgenesis
Phleum pratense
anther culture
genotype
timothy
medium
anther treatment
homozygous line
in vitro culture
haploid
microspore
plant production
Źródło:: Łąkarstwo w Polsce; 2006, 09; 33-40
1506-5162
Pojawia się w:: Łąkarstwo w Polsce
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Gene segregation in a barley DH population
Autorzy:: Szarejko, I
Maluszynski, M
Polok, K
Zbieszczyk, J
Powiązania:: https://bibliotekanauki.pl/articles/2046613.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: doubled haploid
barley
haploid plant
gametoclonal variation
gametic selection
anther culture
population
gene segregation
Opis:: A large population of anther culture-derived barley regenerants and their progeny was tested for allele segregation at 1 isozyme and 8 morphological marker loci. The segregation of genetic markers was examined separately for haploid, diploid and polyploid regenerants. All the 9 analysed genes except al (albino lemma) on chromosome 3 segregated according to the expected 1:1 ratio in the microspore-derived barley population. There was no difference in allele distribution between haploid and diploid regenerants. Among the limited number of 34 analysed tetraploids a significant excess of the dominant allele at locus о (orange lemma) of chromosome 6 was also observed. The recombination frequency between linked genes (n - lk2 on chromosome 1 and r - s on chromosome 7) estimated in the DH population did not differ significantly from recombination rates calculated in F₂ progeny or presented in barley chromosome maps. The phenomenon of gametic selection is discussed in relation to the genotype dependency of anther culture response and procedures used for DH production in barley.
Źródło:: Journal of Applied Genetics; 1997, 38, 4; 415-424
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Factors influencing the efficiency of wheat anther culture
Autorzy:: Lantos, C.
Pauk, J.
Powiązania:: https://bibliotekanauki.pl/articles/2117772.pdf
Data publikacji:: 2021
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: androgenesis
anther culture
doubled haploid
Triticum aestivum L.
wheat
Opis:: Using in vitro androgenesis serves as a unique opportunity to produce doubled haploid (DH) plants in many species. More benefits of this biological phenomenon have kept these methods in the focus of fundamental research and crop breeding for decades. In common wheat (Triticum aestivum L.), in vitro anther culture is one of the most frequently applied DH plant production methods. The efficiency of in vitro wheat anther culture is influenced by many factors, such as the genotype, growing conditions, collection time, pre-treatments, and compositions of media and culture conditions. According to some critical review, the genotype dependency, low efficiency and albinism are mentioned as limitations of application of the anther culture method. However, some research groups have made significant efforts to diminish the effects of these bottlenecks. Due to the improvements, a well-established in vitro anther culture method can be an efficient tool in modern wheat breeding programs.
Źródło:: Acta Biologica Cracoviensia. Series Botanica; 2020, 62, 2; 7-16
0001-5296
Pojawia się w:: Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 15.

Tytuł:: Evaluation of the regeneration ability of Brassica interspecific hybrids in in vitro anther culture
Autorzy:: Wojciechowski, A.
Niemann, J.
Powiązania:: https://bibliotekanauki.pl/articles/951274.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: anther culture
haploid plant
homozygous line
interspecific hybrid
Brassica
regeneration ability
in vitro condition
Źródło:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:: BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:: Biblioteka Nauki

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