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Tytuł:
The Mechanical Properties and Wear Resistance of HVOF Sprayed WC-Co Coatings
Autorzy:
Özbek, Y.
Canikoğlu, N.
İpek, M.
Powiązania:
https://bibliotekanauki.pl/articles/1187418.pdf
Data publikacji:
2016-04
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
81.15.Rs
Opis:
In this work, the Woka 5810 powders (88% tungsten carbide-12% cobalt) were used to produce coating by high velocity oxy-fuel spraying. WC-Co is widely used as a tribological coating material providing a combination of high toughness, high hardness, and good strength. The treated samples were characterized by using optical micrograph, stereo microscope and scanning electron microscopy, X-ray diffractometry, and microhardness tests. Also the wear performance of the coatings was investigated. The results indicated that the coating shows slight higher microhardness and better abrasive wear resistance than the conventional counterpart. The friction coefficient of coating was low. The scanning electron microscopy and energy dispersive spectroscopy analyses were applied to worn surfaces.
Źródło:
Acta Physica Polonica A; 2016, 129, 4; 600-603
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
R.S. Peters’ comprehensive theory of moral education
Autorzy:
Stefaan, Cuypers,
Powiązania:
https://bibliotekanauki.pl/articles/891962.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Warszawski. Wydawnictwa Uniwersytetu Warszawskiego
Tematy:
R.S. Peters
Opis:
This article presents R.S. Peters’ theory of moral education embedded in his broad conception of morality. The author examines Peters’ views against the background of Kohlberg’s stage theory of moral development; hence, the positions of both thinkers are interwoven throughout the discussion. It addresses some central issues relevant to moral education such as, for example: cognitive and affective aspects of morality, and the acquisition of virtues. In the article the author argues that Peters’ account of moral development and moral education provides supplementation for the somewhat narrow theory developed by Kohlberg, thus establishing a broader framework relevant to moral education.
Źródło:
Kultura Pedagogiczna. Międzynarodowe Pismo Pedagogiczne; 2014, 01(1); 11-27
2391-9175
2450-1816
Pojawia się w:
Kultura Pedagogiczna. Międzynarodowe Pismo Pedagogiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The role of MGMT polymorphisms rs12917 and rs11016879 in head and neck cancer risk and prognosis
Autorzy:
Kiczmer, Paweł
Prawdzic Seńkowska, Alicja
Strzelczyk, Joanna
Szydło, Błażej
Biernacki, Krzysztof
Osadnik, Tadeusz
Krakowczyk, Łukasz
Ostrowska, Zofia
Powiązania:
https://bibliotekanauki.pl/articles/1038528.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
MGMT
head and neck cancer
polymorphism
rs12917
rs11016879
Opis:
Head and neck squamous cell carcinoma (HNSCC) is one of the leading cancers by incidence worldwide. The risk of these cancers is strictly associated with alkylation factors present in tobacco smoke. The crucial role in preventing DNA alkylation is played by O6-methylguanine-DNA methyltransferase (MGMT). Dysfunction or lack of MGMT is associated with an increased risk of cancer. The aim of the study was to assess the influence of MGMT polymorphisms: rs12917 and rs11016879 on HNSCC risk and course. The study consisted of 69 HNSCC patients and 242 healthy individuals. Case samples were taken from resected tumour tissue. The control group comprised samples of epithelial cells collected from mucous membranes using swabs. DNA samples were genotyped by employing the 5' nuclease assay for allelic discrimination using TaqMan SNP Genotyping Assays. The significance between distributions of genotypes and alleles was tested using Pearson's χ2 test analysis. Our results indicated that the MGMT rs12917 TT genotype increases the risk of HNSCC. The MGMT rs11016879 AG genotype and A allele were associated with increased HNSCC risk. We noted higher risk of nodal metastasis in rs11016879 AA homozygotes. Mechanisms leading to MGMT enzymatic defect are unknown and hence further studies need to be carried out. Our data suggest that the examined polymorphisms may be considered as potential prognostic factors for HNSCC risk and outcome. Further studies are necessary to verify our results.
Źródło:
Acta Biochimica Polonica; 2018, 65, 1; 87-92
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Surface Properties of WC-Co-Cr Based Coatings Deposited by High Velocity Oxygen Fuel Spraying
Autorzy:
Ozbek, Y.
Canikoğlu, N.
İpek, M.
Powiązania:
https://bibliotekanauki.pl/articles/1032535.pdf
Data publikacji:
2017-01
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
81.15.Rs
Opis:
The aim of this research was to investigate microstructural and mechanical properties of the WC-Co-Cr coatings by high velocity oxygen fuel spraying. Woka 3653 (WC10Co4Cr) powder was used as coating material. This powder is widely used as a tribological coating material providing a combination of high toughness, high hardness, and good strength. The coatings were produced for the different high velocity oxygen fuel spraying parameters. The treated samples were characterized by using scanning electron microscopy/energy dispersive X-ray spectrometry and X-ray diffractometry. Microhardness measurements were executed to evaluate the mechanical properties of the coatings. Also the wear performance of the coatings was investigated. The scanning electron microscopy and energy dispersive X-ray spectrometry analyses were applied to worn surfaces. The results indicated that the coating shows slightly higher microhardness and better abrasive wear resistance than the conventional counterpart.
Źródło:
Acta Physica Polonica A; 2017, 131, 1; 186-189
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes in Microstructure of Air Plasma Sprayed M-CrAlY Coatings After Short Thermal Exposure in Argon Atmosphere
Autorzy:
Čelko, L.
Řičánková, V.
Klakurková, L.
Podrábský, T.
Dvořáček, E.
Švejcar, J.
Powiązania:
https://bibliotekanauki.pl/articles/1503728.pdf
Data publikacji:
2011-08
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
81.15.Rs
Opis:
Conventional air plasma spray technique and two powders, NiCrAlY and CoNiCrAlY, were used to produce the M-CrAlY type of coating on the Inconel 713LC substrate. Two phase coatings, consisting of Ni and/or Co solid solution and NiAl intermetallic phase, were produced. A certain amount of imperfectly melted powder particles, voids and aluminium oxide was also present. After air plasma spraying the coatings were annealed at widely ranging temperatures (650, 800, 1000 and 1150°C) for two hours in argon-flow atmosphere. It was found that the temperature significantly affects the microstructure of resulting coatings. The oxide scale was formed by internal oxidation in a coating region primarily at higher temperatures. In this case, aluminium was depleted from the NiAl phase within the coating region and the Ni, Co, Cr solid solutions and the aluminium oxide started to form rapidly. No interaction was observed after the short thermal exposure below the substrate surface. The microstructure of coatings was recorded by scanning electron microscope. Coating thickness, amount of voids and oxide scale were measured by means of image analysis. The concentration of phases was estimated by energy dispersive microanalysis.
Źródło:
Acta Physica Polonica A; 2011, 120, 2; 336-339
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Thermomagnetic Behaviour and Microstructure of a Rapidly Quenched $Nd_{4.5}Fe_{77}B_{18.5}$ Alloy
Autorzy:
Talijan, N.
Ćosović, V.
Grujić, A.
Stajić-Trošić, J.
Žák, T.
Powiązania:
https://bibliotekanauki.pl/articles/1813871.pdf
Data publikacji:
2008-01
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
81.40.Rs
Opis:
Changes in the phase composition and crystallite size, as well as changes in the magnetic behaviour of $Nd_{4.5}Fe_{77}B_{18.5}$ alloy, caused by thermomagnetic measurements, was observed in regard of the optimal magnetic state of this alloy. In the optimized magnetic state, the formed nanocomposite consisted of $Fe_3B//Nd_2Fe_{14}B$ and partly of α-Fe with a mean crystallite size < 30 nm, as determined by X-ray diffraction and transmission electron microscopy. An increased amount of α-Fe, the presence of $Nd_2O_3$ and different Fe-B phases, as well as an increase in the mean crystallite size were observed after thermomagnetic measurements had caused a quality loss of the hard magnetic properties.
Źródło:
Acta Physica Polonica A; 2008, 113, 1; 525-528
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wybrane polimorfizmy genu VEGF w przewlekłej chorobie nerek
Selected polymorphisms of VEGF gene in chronic renal failure
Autorzy:
Żywiec, Joanna
Kiliś-Pstrusińska, Katarzyna
Grzeszczak, Władysław
Powiązania:
https://bibliotekanauki.pl/articles/1035569.pdf
Data publikacji:
2018
Wydawca:
Śląski Uniwersytet Medyczny w Katowicach
Tematy:
vegf-a
przewlekła choroba nerek
rs699947
rs1570360
chronic kidney disease
Opis:
INTRODUCTION: Understanding the complex etiopathogenesis of chronic kidney disease (CKD) raises hopes for the development of effective methods for its early diagnosis and effective prevention and treatment. The significance of genetic predisposition in renal damage development has been proven in many studies. The objective of our study was to estimate the connections between selected VEGF polymorphisms and the incidence of chronic renal failure. MATERIALS AND METHODS: 103 families, consisting of both parents and affected offspring with CKD stage below 3, i.e. eGFR below 60 ml/min/1.73 m2, participated our study. The mean age of the children was 15.2 years and mean eGFR 28.9 ml/min/1.73 m2. The main reason for renal damage was interstitial nephropathy (71%) and the second (29%) – chronic glomerulonephritis. 45% of patients were conservatively treated, while others remained on chronic renal replacement therapy. Venous blood samples were collected for DNA isolation. Selected VEGF polymorphisms were genotyped by the TaqMan method. The obtained results were analyzed on the basis of Statistica 10 and Microsoft Office Excel 2003 software using the transmission disequilibrium test (TDT), χ2 test and the Kaplan-Meier estimator. For all the calculations, a p value below 0.05 was adopted as the limit of statistical significance. RESULTS: No differences in the estimated and observed rs699947 or rs1570360 allele transmission between the parents and their affected offspring were found. CONCLUSIONS: No relations between rs699947 and rs1570360 VEGF polymorphisms and the incidence of chronic kidney disease of at least stage 3 were established.
WSTĘP: Poznanie złożonej etiopatogenezy przewlekłej choroby nerek (PChN) budzi nadzieje na rozwój skutecznych metod jej wczesnej diagnostyki oraz skutecznej profilaktyki i leczenia. Liczne badania dowodzą znaczenia predyspozycji genetycznej w rozwoju przewlekłego uszkodzenia nerek w przebiegu różnych chorób. Celem pracy była ocena związku wybranych polimorfizmów genu VEGF (vascular endothelial growth factor) z występowaniem przewlekłej choroby nerek. MATERIAŁ I METODY: Grupę badaną stanowiły 103 rodziny (trios): dwoje biologicznych rodziców i dziecko z PChN w stadium co najmniej trzecim, tj. z eGFR ≤ 60 ml/min/1,73 m2, na tle przewlekłego kłębuszkowego zapalenia nerek (29%) lub nefropatii śródmiąższowej (71%). W chwili badania średni wiek dzieci z PChN wynosił 15,2 roku, a średnia filtracja kłębuszkowa 28,9 ml/min/1,73 m2. U 45% chorych zastosowano leczenie zachowawcze, u pozostałych nerkozastępcze. U wszystkich badanych pobrano jednorazowo próbki krwi żylnej w celu wyizolowania materiału genetycznego i przeprowadzono genotypowanie wybranych polimorfizmów genu VEGF metodą TaqMan. Do opracowania statystycznego wyników posłużyły programy Statistica 10 i Microsoft Office Excel 2003 z wykorzystaniem testu nie-równowagi sprzężeń (transmission disequilibrium test – TDT), testu χ2 oraz estymatora Kaplana-Meiera. Za granicę istotności statystycznej przyjęto wartości p < 0,05. WYNIKI: Nie wykazano różnic w przekazywaniu alleli badanych polimorfizmów rs699947 i rs1570360 genu VEGF od rodziców do potomka z PChN. WNIOSKI: Nie stwierdzono związku polimorfizmów rs699947 i rs1570360 genu VEGF z występowaniem przewlek-łej choroby nerek w stadium ≥ 3.
Źródło:
Annales Academiae Medicae Silesiensis; 2018, 72; 267-272
1734-025X
Pojawia się w:
Annales Academiae Medicae Silesiensis
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Simulator for the RS operator using visual depiction
Symulator dla operatora RS wykorzystujący zobrazowanie wizyjne
Autorzy:
Przybylski, M.
Hass, A.
Powiązania:
https://bibliotekanauki.pl/articles/223129.pdf
Data publikacji:
2017
Wydawca:
Akademia Marynarki Wojennej. Wydział Dowodzenia i Operacji Morskich
Tematy:
RS operator simulator
visual depiction
automatic tracking
symulator operatora RS
zobrazowanie wizyjne
automatyczne śledzenie
Opis:
The high operational cost of exploitation of armaments in near-battle conditions and numerous restrictions in use of the battle equipment in places of permanent deployment have contributed to seek new training methods. In recent years, there has been a rapid development of simulators used in the process of education and in-service training associated with the technological advancement and the modernization of the armed forces of the Republic of Poland. In this article, an experimental model of the simulator was introduced for the operator of manual tracking (RS) using a visual system. This model was built by the authors as part of their master’s thesis. It allows either manual or automatic tracking of targets against the simulated background and gives a grade to the operator based on the accuracy achieved while tracking the target. The solution suggested by the authors may raise the effectiveness of training of operators using television-optical systems, as well as reduce the associated operating costs of the weaponry.
Wysokie koszty eksploatacji uzbrojenia w warunkach zbliżonych do bojowych i liczne ograniczenia w użyciu sprzętu bojowego w miejscach stałej dyslokacji przyczyniły się do poszukiwania nowych metod szkolenia. W ostatnich latach nastąpił dynamiczny rozwój symulatorów wykorzystywanych w procesie kształcenia i doskonalenia zawodowego związany z postępem technologicznym oraz modernizacją Sił Zbrojnych RP. W artykule przedstawiono model doświadczalny symulatora dla operatora ręcznego śledzenia (RS) wykorzystujący system wizyjny. Model ten został zbudowany przez autorów w ramach ich prac magisterskich. Umożliwia on ręczne lub automatyczne śledzenie celów na pozorowanym tle oraz wystawienie oceny operatorowi na podstawie osiągniętej dokładności podczas śledzenia celu. Zaproponowane rozwiązanie może podnieść efektywność szkolenia operatorów korzystających z systemów telewizyjno-optycznych oraz obniżyć związane z tym koszty eksploatacji uzbrojenia.
Źródło:
Zeszyty Naukowe Akademii Marynarki Wojennej; 2017, R. 58 nr 3 (210), 3 (210); 87-95
0860-889X
Pojawia się w:
Zeszyty Naukowe Akademii Marynarki Wojennej
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Performance comparison of cylindrical AS/RS with single and multi load handling devices
Cравнение работы автоматизированных цилиндрических систем складирования С-AS/RS с разными погрузочно-разгрузочными устройствами
Autorzy:
Janilionis, V.
Bazaras, Z.
Powiązania:
https://bibliotekanauki.pl/articles/374651.pdf
Data publikacji:
2013
Wydawca:
Politechnika Śląska. Wydawnictwo Politechniki Śląskiej
Tematy:
cylindrical AS/RS
automated warehouse
simulation
statistical analysis
cylindryczne AS/RS
symulacja
analiza statystyczna
Opis:
This paper proposes the concept of a new type of Cylindrical Automated Storage and Retrieval system (C-AS/RS), which consists of the octagonal shape rack, system input/output (I/O) positions, load storage and retrieval mechanism (SRM), which rotates and moves simultaneously and contains a number of load handling devices (LHDs). Physical structure of the single and multi-LHD systems, control algorithms and the performance measures were presented. Simulation modelling techniques, descriptive statistics and analysis of variance were used in this research to identify the effects of different location of I/O positions, the number of LHDs on the SRM and the retrieval request rates to the performance of the C-AS/RS system.
В данной работе описывается концепция aвтоматизированных цилиндрических систем складирования нового типа (англ. Cylindrical Automated Storage and Retrieval Systems - C-AS/RS), которая состоит из стеллажа восьмиугольной формы, системы локализации ввода / вывода (англ. input/output-I / O), механизма сбора и хранения груза (англ. Storage Retrieval Mechanism - SRM), который вращается и движется одновременно и содержит ряд погрузочно-разгрузочных устройств (англ. Load Handling Device - LHD). Также была представленф физическая структура моно и мульти-LHD системы, алгоритмы управления и показатели эффективности. В данном исследовании были использованы методы иммитационного моделирования, дискриптивной статистики и дисперсионного анализа для определения влияния различных I/O локализаций, количества LHDs на SRM и интенсивности потока выбора груза на производительность системы C-AS/RS.
Źródło:
Transport Problems; 2013, 8, 4; 93-102
1896-0596
2300-861X
Pojawia się w:
Transport Problems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diagnostic information model of a military vehicle
Autorzy:
Rychlik, A.
Szczyglak, P.
Powiązania:
https://bibliotekanauki.pl/articles/244455.pdf
Data publikacji:
2011
Wydawca:
Instytut Techniczny Wojsk Lotniczych
Tematy:
transport
diagnostic
transmission system
RS-485
Opis:
This study presents a diagnostic concept, the cybernetic structure and the physical model of the Autonomic Logistics System, a diagnostic sub-system implemented in the Tarpan Honker military vehicle. The system supports the driver in the process of making decisions regarding the vehicle's condition and key operating parameters. The information model reporting on the status and faults of power transmission system components will not identify limit state values. Limit state values will be identified after the vehicle has been tested with an implemented diagnostic system. An RS-485 type communication and data transmission system with linear (serial) half-duplex technology was designed for the proposed monitoring and diagnostic system. The developed data transmission topology (number of transmitters and receivers) accounts for the vehicle's unique structure, operating requirements, system functionalities and structural feasibility. The proposed transmission system supports quick data transfer between modules (devices) monitoring selected systems and mechanisms. The system minimizes the number of sensors and signal cables through the multiple use of measuring sensor signals. The proposed system is an open structure which supports data transfer between control devices supplied by different manufacturers;
Źródło:
Journal of KONES; 2011, 18, 1; 509-514
1231-4005
2354-0133
Pojawia się w:
Journal of KONES
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study of the Hydrogen-Metal Systems
Autorzy:
Nikitenkov, N.
Hashhash, A.
Sigfusson, T.
Kudryavtzeva, E.
Sypchenko, V.
Tyurin, Yu.
Chernov, I.
Powiązania:
https://bibliotekanauki.pl/articles/1490775.pdf
Data publikacji:
2012-01
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
68.43.Nr
68.43.Rs
Opis:
Hydrogen accumulation in samples of a palladium and 12Kh18N10T steel at the hydrogen charging by the electrolytic method and hydrogen release from these samples at its electron and X-ray irradiation are studied. Palladium was used as a comparison material (as most efficiently solvent hydrogen known among the simple materials). It is established that a capture effectiveness of hydrogen from an electrolyte (1 M $H_2SO_4$ at current density is 0.5 A $cm^{-2}$) for palladium is 3-4 orders more than for steel. The hydrogen yield nonlinearly increases with growing of electron current density and electron energy is more than 40 keV under electron irradiation of saturated palladium and 12Kh18N10T steel samples. About 90% of the hydrogen had removed from hydrogen saturated palladium samples and only 60% from steel under electron beam with energy 40 keV and current density ≈ 20 μA $cm^{-2}$ for 1 h of irradiation. It is necessary to increase the energy of electrons from 40 to 100 keV for the more effective removal of hydrogen.
Źródło:
Acta Physica Polonica A; 2012, 121, 1; 78-81
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Speed of Ultrasound and Internal Pressure of Propanediol and Butanediol Isomers under Elevated Pressures
Autorzy:
Zorębski, E.
Piotrowska, M.
Dzida, M.
Powiązania:
https://bibliotekanauki.pl/articles/1811570.pdf
Data publikacji:
2008-12
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
82.30.Rs
62.50.-p
Opis:
Speed of ultrasound and internal pressure of 1,2-propanediol, 1,3-propanediol, 1,2-butanediol, and 1,3-butanediol at the temperatures ranging from 293.15 to 318.15 K and pressures up to 101 MPa are analyzed and discussed in terms of molecular structure and ability to form inter- and intramolecular hydrogen bonds.
Źródło:
Acta Physica Polonica A; 2008, 114, 6A; A-265-A-270
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
AFM Force Spectroscopy and Steered Molecular Dynamics Simulation of Protein Contactin 4
Autorzy:
Strzelecki, J.
Powiązania:
https://bibliotekanauki.pl/articles/1807636.pdf
Data publikacji:
2009-12
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
82.37.Rs
87.10.Tf
Opis:
We use a single molecule atomic force spectroscopy combined with the steered molecular dynamics simulation to determine a mechanical behavior of neural cell adhesion protein contactin during its unfolding. Force curves typical for modular proteins were observed, showing at most four unfolding peaks. The analysis of force spectra performed within worm-like chain model of polymer elasticity showed the presence of three unfolding lengths. Small plateaus, most likely resulting from forced transitions within domains were observed for the first time. Steered molecular dynamics simulations help to determine atomistic picture of domain unfolding.
Źródło:
Acta Physica Polonica A; 2009, 116, S; S-156-S-159
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of High Power Laser Beam on Physical Properties of Epitaxial Films of Hg$\text{}_{1-x}$Cd$\text{}_{x}$Te (x≈0.2)
Autorzy:
Kuźma, M.
Abeynayake, C.
Sheregii, E.
Virt, I.
Powiązania:
https://bibliotekanauki.pl/articles/1891472.pdf
Data publikacji:
1991-09
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
73.60.Cs
81.40.Rs
Opis:
Models have been proposed explaining differences in electrophysical and photoelectrical properties of epitaxial films of Hg$\text{}_{1-x}$Cd$\text{}_{x}$Te (x≈0.2) before and after treating by single pulses of Nd:YAG laser. These models were obtained on the basis of the calculated distributions of temperature, temperature gradient and concentration of interstitial mercury during and after exposing to the laser pulse. These calculations were done using the net method.
Źródło:
Acta Physica Polonica A; 1991, 80, 3; 475-479
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Force Spectroscopy of Polyclonal and Monoclonal Anti-Bovine Serum Albumin Antibodies - BSA Complexes
Autorzy:
Chtcheglova, L. A.
Dietler, G.
Powiązania:
https://bibliotekanauki.pl/articles/2036898.pdf
Data publikacji:
2003
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
82.37.Gk
82.37.Rs
Opis:
The specific interactions between bovine serum albumin and poly- or two monoclonal bovine serum albumin antibodies were studied using force spectroscopy mode of atomic force microscopy. The histograms of the unbinding forces for polyclonal bovine serum albumin antibodies are broad at high antibody concentrations (50 or 270 μg/ml) and narrow at low concentrations (10 or 27 μg/ml), while the histograms for monoclonal antibodies peak at well defined unbinding force. The peak unbinding force depends on the type of antibody and the antibody concentration. In this paper we described and characterized the passive adsorption and covalent immobilization of proteins for tip and sample preparation. Force spectroscopy could serve as a useful method for characterization of antigen-antibody interactions for measuring the specificity of an antibody or to assess the purity of a monoclonal antibody solution and to distinguish between different antibodies.
Źródło:
Acta Physica Polonica A; 2003, 104, 3-4; 321-326
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 128

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