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    Wyświetlanie 1-5 z 5
    Tytuł:
    Identification of viruses infecting cucurbits and determination of genetic diversity of cucumber mosaic virus in Lorestan province, Iran
    Autorzy:
    Hasanvand, V.
    Shams-bakhsh, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/66238.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    identification
    virus
    cucurbit
    determination
    genetic diversity
    cucumber mosaic virus
    RFLP analysis
    Lorestan province
    Iran
    Opis:
    Various viral pathogens infect Cucurbitaceae and cause economic losses. Th e aim of the present study was to detect plant viral pathogens including Cucumber mosaic virus (CMV), Cucumber green mottle mosaic virus (CGMMV), Zucchini yellow mosaic virus (ZYMV), Cucurbit yellow stunting disorder virus (CYSDV) and Cucurbit chlorotic yellows virus (CCYV) in Lorestan province, in western Iran, and also to determine CMV genetic diversity in Iranian populations. A total of 569 symptomatic leaf samples were collected in 2013 and 2014 from cucurbits growing regions in Lorestan province. Th e collected samples were assessed for viral diseases by ELISA. Th e results showed virus incidences in most regions. Th en, the infection of 40 samples to CMV was confi rmed by RT-PCR. Moreover, to distinguish between the two groups (I and II) of CMV, PCR products were digested by two restriction enzymes XhoI and EcoRI. Results of the digestion showed that the isolates of Lorestan belonged to group I. Th e CMV-coat protein gene of eight isolates from diff erent regions and hosts was sequenced and phylogenetic analysis was performed. Subsequent analyses showed even more genetic variation among Lorestan isolates. Th e phylogenetic tree revealed that Lorestan province isolates belonged to two IA and IB subgroups and could be classifi ed together with East Azerbaijan province isolates. Th e results of the present study indicate a wide distribution of CMV, ZYMV, CGMMV, CYSDV and CCYV viruses in cucurbits fi elds of Lorestan province and for the fi rst time subgroup IB of CMV was reported on melon from Iran.
    Źródło:
    Journal of Plant Protection Research; 2017, 57, 2
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Utility of two mitochondrial markers for identification of Picea abies refugial origin
    Autorzy:
    Litkowiec, M
    Dering, M.
    Lewandowski, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/41333.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    coniferous plant
    tree
    Norway spruce
    Picea abies
    mtDNA
    molecular marker
    mitochondrial marker
    identification
    polymerase chain reaction
    RFLP analysis
    gene pool conservation
    forest ecosystem
    plant population
    Opis:
    Picea abies (L.) Karst is one of the most important coniferous species of Europe from both ecological and economical points of view. Traditional methods for the gene pool conservation and biodiversity maintenance in forest ecosystems have been practiced in many countries. For progress in this field using highly polymorphic genetic molecular markers is needed. Our goal was to demonstrate the utility of two polymorphic mitochondrial markers mt15-D02 and nad1 b/c in identification native Norway spruce stands. This molecular markers were tested in 1401 individuals from 59 Polish Norway spruce populations. We detected three alleles, which are called1, 2 and3, for locus mt15-D02 and two alleles , which are called1 and2, for locus nad1 b/c in our material. All five variants of alleles indicate the natural origin of P. abies. Result of this study shows that molecular marker mt15-D02 is easy to use and more informative in compare to marker nad1 b/c.
    Źródło:
    Dendrobiology; 2009, 61; 65-71
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic polymorphism of Polish strains of Gremmeniella abietina and Brunchorstia pinea var. cembrae
    Autorzy:
    Kraj, W
    Powiązania:
    https://bibliotekanauki.pl/articles/41508.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    coniferous tree
    plant pathology
    pine
    spruce
    fir
    juniper
    pathogen
    plant disease
    shoot blight
    canker
    branch
    stem
    Gremmeniella abietina
    Brunchorstia pinea var.cembrae
    needle
    genetic polymorphism
    Polish strain
    host preference
    polymerase chain reaction
    RFLP analysis
    Opis:
    Thirty-three type A strains of G. abietina from diseased shoots or needles of P. sylvestris, P. nigra and P. armandii and three strains of Brunchorstia pinea var. cembrae from P. mugo were isolated from four regions of Poland differing with respect to climatic conditions. Genetic polymorphism of the mitochondrial small subunit rRNA (mtSSU rRNA), ribosomal RNA fragment including ITS1, 5.8S and ITS2 and glyceraldehyde phosphate dehydrogenase (GPD) gene was examined by the PCR-RFLP method. Genetic distance was ascertained with respect to B. pinea var. cembrae strains from G. abietina isolated from the examinedpine species (average Nei coefficient 0.137). The smallest genetic distance occurred between the strain groups of G. abietina isolated from P. nigra and P. armandii (0.059) and P. nigra and P. sylvestris (0.061), whereas the highest occurred between the groups of strains deriving from P. armandii and P. sylvestris (0.096). The impact of geographic distance on genetic distance between groups of strains from individual regions has been shown. G. abietina strains originating from mountainous areas were more distanced genetically (on average 0.031) from populations from other regions (Nei genetic distance 0.023). The main factors influencing genetic differences of the pathogen were specificity with respect to the species of the host plant and climate conditions, whereas geographic distance had lesser significance.
    Źródło:
    Dendrobiology; 2009, 61; 13-21
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular characterization of ‘Candidatus Phytoplasma mali’ strains from Bulgaria and Poland
    Autorzy:
    Cieślińska, M.
    Borisova, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/12308151.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Polska
    Bulgaria
    apple proliferation phytoplasma
    molecular characteristics
    Candidatus Phytoplasma mali
    polymerase chain reaction
    RFLP method
    multilocus gene analysis
    genetic diversity
    Opis:
    During 2015, samples from 22 apple trees showing proliferation symptoms were collected in southwest Bulgaria and Central and South Poland and tested for phytoplasma presence. ‘Candidatus Phytoplasma mali’ was identified in 18 samples based on results of restriction fragment length polymorphism (RFLP) analysis of the 16S rRNA gene amplified in nested PCR using primer pair P1/P7 followed by R16F2n/R16R2 and F1/B6 primer pairs. The nitroreductase and rhodonase-like genes and ribosomal protein genes rpl22 and rps3 were then analyzed using PCR-RFLP technique to study the genetic variability of the phytoplasma strains. Two restriction profiles, P-I or P-II, were obtained from fragments of 16S rDNA plus 16S-23S spacer region digested with HpaII enzyme. Restriction fragment length polymorphism analysis of nitroreductase and rhodonase-like genes using digestion with HincII endonuclease revealed that all ‘Ca. P. mali’ strains belonged to the subtype AP-15. Analysis of rpl22 and rps3 ribosomal protein genes digested with AluI enzyme resulted in classification of detected phytoplasma strains to rpX-A subgroup.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 5; 181-188
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis
    Autorzy:
    Karakulska, J.
    Pobucewicz, A.
    Nawrotek, P.
    Muszynska, M.
    Furowicz, A.J.
    Czernomysy-Furowicz, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/31963.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    molecular typing
    Staphylococcus aureus
    PCR-RFLP method
    coa gene
    random amplified polymorphic DNA analysis
    mastitis
    nuc gene
    gap gene
    milk sample
    species identification
    Opis:
    The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.
    Źródło:
    Polish Journal of Veterinary Sciences; 2011, 14, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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