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Tytuł:
Lipopolysaccharide (LPS, endotoxin) of Proteus bacteria-chemical structure, serological specificity and the role in pathogenicity
Autorzy:
Różalski, Antoni
Powiązania:
https://bibliotekanauki.pl/articles/764987.pdf
Data publikacji:
2008
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:
bacterial lipopolysaccharide
endotoxin
Proteus
Opis:
Lipopolysaccharides (LPS) of Gram-negative bacteria are composed of three regions: О-specific chain (OPS), the core oligosaccharide and lipid A. All three regions of Proteus LPS were studied. The differences in the structure of OPS serve as the basis for the serological classification of Proteus strains. The serological classification scheme of these bacteria currently consists of 76 serogroups. The structural diversity of the core region is characteristic for Proteus sp. and distinguishes this genus from other bacteria. In this paper the results of structural, immunochemical and serological studies of all three regions o f Proteus LPS, as well as a function of LPS as endotoxin and its role in the formation of urinary stones, swarming phenomenon and bacterial growth in biofilm are reported.
Źródło:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2008, 4
1730-2366
2083-8484
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteus sp. – an opportunistic bacterial pathogen – classification, swarming growth, clinical significance and virulence factors
Autorzy:
Różalski, Antoni
Torzewska, Agnieszka
Moryl, Magdalena
Kwil, Iwona
Maszewska, Agnieszka
Ostrowska, Kinga
Drzewiecka, Dominika
Zabłotni, Agnieszka
Palusiak, Agata
Siwińska, Małgorzata
Stączek, Paweł
Powiązania:
https://bibliotekanauki.pl/articles/764854.pdf
Data publikacji:
2012
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:
Proteus
pathogenicity
mechanisms of virulence
Opis:
The genus Proteus belongs to the Enterobacteriaceae family, where it is placed in the tribe Proteeae, together with the genera Morganella and Providencia. Currently, the genus Proteus consists of five species: P. mirabilis, P. vulgaris, P. penneri, P. hauseri and P. myxofaciens, as well as three unnamed Proteus genomospecies. The most defining characteristic of Proteus bacteria is a swarming phenomenon, a multicellular differentiation process of short rods to elongated swarmer cells. It allows population of bacteria to migrate on solid surface. Proteus bacteria inhabit the environment and are also present in the intestines of humans and animals. These microorganisms under favorable conditions cause a number of infections including urinary tract infections (UTIs), wound infections, meningitis in neonates or infants and rheumatoid arthritis. Therefore, Proteus is known as a bacterial opportunistic pathogen. It causes complicated UTIs with a higher frequency, compared to other uropathogens. Proteus infections are accompanied by a formation of urinary stones, containing struvite and carbonate apatite. The virulence of Proteus rods has been related to several factors including fimbriae, flagella, enzymes (urease - hydrolyzing urea to CO2 and NH3, proteases degrading antibodies, tissue matrix proteins and proteins of the complement system), iron acqusition systems and toxins: hemolysins, Proteus toxin agglutinin (Pta), as well as an endotoxin - lipopolysaccharide (LPS). Proteus rods form biofilm, particularly on the surface of urinary catheters, which can lead to serious consequences for patients. In this review we present factors involved in the regulation of swarming phenomenon, discuss the role of particular pathogenic features of Proteus spp., and characterize biofilm formation by these bacteria.
Źródło:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2012, 8; 1-17
1730-2366
2083-8484
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of ciprofloxacin and gentamicin diffusion in Proteus mirabilis O18 biofilm by laser interferometry method
Autorzy:
Arabski, Michał
Wąsik, Sławomir
Zych, Magdalena
Łakomiec, Wioletta
Kaca, Wiesław
Powiązania:
https://bibliotekanauki.pl/articles/1039471.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
laser interferometry
Proteus sp.
biofilm
ciprofloxacin
gentamicin
Opis:
Laser interferometry is a measurement technique used in physical sciences, with a potential for new applications in microbiology. Our previously studies, focused on the quantitative analysis of antibiotics diffusion through membranes or their releasing from gel structure, indicate that this method might be useful in analysis of substances diffusion across the bacterial biofilms. As antibiotic - biofilm interaction model, we tested above method for determination of ciprofloxacin or gentamicin diffusion through Proteus mirabilis O18 biofilm. Laser interferometry analysis of antibiotics diffusion showed that the amount of ciprofloxacin transported through mature biofilm is 1.9 times higher than gentamicin. It was correlated with lower level of gentamicin in compare to the level of ciprofloxacin in biofilm, which amounts were predicted in biofilm during diffusion process by laser interferometry method. We suggest that the analysis of antibiotic diffusion in biofilm might by helpful in evaluation of effectiveness of antibacterial agents.
Źródło:
Acta Biochimica Polonica; 2013, 60, 4; 707-711
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of epitope specificity of Proteus penneri 7 lipopolysaccharide core region
Autorzy:
Palusiak, Agata
Sidorczyk, Zygmunt
Powiązania:
https://bibliotekanauki.pl/articles/1040317.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
epitope
lipopolysaccharide
Proteus penneri
core region
Opis:
To extend the knowledge on the fragments of Proteus penneri lipopolysaccharide core regions, which determine the cross-reactions with specific antibodies, serological studies were performed by use of P. penneri 7 core-specific antiserum and Proteus sp. lipopolysaccharides. Different reactivity of the tested antiserum with three groups of antigens suggested differences in their core regions' epitope specificity. Comparing the results of the serological investigations with the previously determined structures of the core regions of the tested P. penneri lipopolysaccharides allowed distinguishing two potential tri- and tetrasaccharide epitopes and a third fragment which could not be determined precisely.
Źródło:
Acta Biochimica Polonica; 2010, 57, 4; 529-532
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zastosowanie stałego podłoża z mocznikiem do ilościowego oznaczania pałeczek z rodzaju Proteus w wyrobach garmażeryjnych
Primenenie plotnojj pitatelnojj sredy s mochevinojj dlja kolichestvennogo opredelenija palochek tipa Proteus
The use of stable medium with urea for Proteus bacilli counting in some dishes and half products
Autorzy:
Burzynska, H.
Powiązania:
https://bibliotekanauki.pl/articles/875483.pdf
Data publikacji:
1962
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
mikroorganizmy
Proteus
bakterie proteolityczne
oznaczanie ilosciowe
wyroby garmazeryjne
Proteus mirabilis
microorganism
proteolytic bacteria
quantitative determination
delicatessen product
Źródło:
Roczniki Państwowego Zakładu Higieny; 1962, 13, 3
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of nutrient and stress factors on polysaccharides synthesis in Proteus mirabilis biofilm
Autorzy:
Moryl, Magdalena
Kaleta, Aleksandra
Strzelecki, Kacper
Różalska, Sylwia
Różalski, Antoni
Powiązania:
https://bibliotekanauki.pl/articles/1039353.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Proteus mirabilis
exopolysaccharides
biofilm
nutrient
stress factors
Opis:
The extracellular matrix in biofilm consists of water, proteins, polysaccharides, nucleic acids and phospholipids. Synthesis of these components is influenced by many factors, e.g. environment conditions or carbon source. The aim of the study was to analyse polysaccharides levels in Proteus mirabilis biofilms after exposure to stress and nutritional conditions. Biofilms of 22 P. mirabilis strains were cultivated for 24, 48, 72 hours, 1 and 2 weeks in tryptone soya broth or in modified media containing an additional amount of nutrients (glucose, albumin) or stress factors (cefotaxime, pH 4, nutrient depletion). Proteins and total polysaccharides levels were studied by Lowry and the phenol-sulphuric acid methods, respectively. Glycoproteins levels were calculated by ELLA with the use of selected lectins (WGA and HPA). For CLSM analysis dual fluorescent staining was applied with SYTO 13 and WGA-TRITC. In optimal conditions the levels of polysaccharides were from 0 to 442 μg/mg of protein and differed depending on the strains and cultivation time. The agents used in this study had a significant impact on the polysaccharides synthesis in the P. mirabilis biofilm. Among all studied components (depending on tested methods), glucose and cefotaxime stimulated the greatest production of polysaccharides by P. mirabilis strains (more than a twofold increase). For most tested strains the highest amounts of sugars were detected after one week of incubation. CLSM analysis confirmed the overproduction of N-acetyloglucosamine in biofilms after cultivation in nutrient and stress conditions, with the level 111-1134%, which varied depending on the P. mirabilis strain and the test factor.
Źródło:
Acta Biochimica Polonica; 2014, 61, 1; 133-139
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Various intensity of Proteus mirabilis-induced crystallization resulting from the changes in the mineral composition of urine
Autorzy:
Torzewska, Agnieszka
Różalski, Antoni
Powiązania:
https://bibliotekanauki.pl/articles/1039148.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Proteus mirabilis
crystallization
urinary stones
mineral composition
Opis:
Infectious urolithiasis is a result of recurrent and chronic urinary tract infections caused by urease-positive bacteria, especially Proteus mirabilis. The main role in the development of this kind of stones is played by bacterial factors such as urease and extracellular polysaccharides, but urinary tract environment also contributes to this process. We used an in vitro model to establish how the changes in the basic minerals concentrations affect the intensity of crystallization which occurs in urine. In each experiment crystallization was induced by an addition of P. mirabilis to artificial urine with a precisely defined chemical composition. Crystallization intensity was determined using the spectrophotometric microdilution method and the chemical composition of formed crystals was established by atomic absorption spectroscopy and colorimetric methods. Increasing the concentration of all crystals forming ions such as Mg2+, Ca2+ and phosphate strongly intensified the process of crystallization, whereas reducing the amount of these components below the proper physiological concentration did not affect its intensity. The inhibitory influence of citrate on calcium and magnesium phosphate crystallization and competitive actions of calcium and oxalate ions on struvite crystals formation were not confirmed. In the case of infectious stones the chemical composition of urine plays an important role, which creates a necessity to support the treatment by developing a model of proper diet.
Źródło:
Acta Biochimica Polonica; 2015, 62, 1; 127-132
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteus mirabilis RMS 203 as a new representative of the O13 Proteus serogroup
Autorzy:
Palusiak, Agata
Siwińska, Małgorzata
Zabłotni, Agnieszka
Powiązania:
https://bibliotekanauki.pl/articles/1038887.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cross-reaction
lipopolysaccharide
O-polysaccharide
O serogroup
Proteus
Opis:
The unique feature of some Proteus O-polysaccharides is occurrence of an amide of galacturonic acid with Nε-[(S/R)-1-Carboxyethyl]-l-lysine, GalA6(2S,8S/R-AlaLys). The results of the serological studies presented here, with reference to known O-antigens structures suggest that GalA6(2S,8S/R-AlaLys) or 2S,8R-AlaLys contribute to cross-reactions of O13 Proteus antisera, and Proteeae LPSs. It was also revealed that the Proteus mirabilis RMS 203 strain can be classified into the O13 serogroup, represented so far by two strains: Proteus mirabilis 26/57 and Proteus vulgaris 8344. The O13 LPS is a serologically important antigen with a fragment common to LPSs of different species in the Proteeae tribe.
Źródło:
Acta Biochimica Polonica; 2015, 62, 4; 691-695
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Powstawanie histaminy w mięsie ryb zakażonym pałeczkami z gatunku Proteus morgan
Histamine development ln the meat of fish contaminated with proteus morgani organisms
Autorzy:
Ganowiak, Z.
Gajewska, R.
Lebiedzinska, A.
Powiązania:
https://bibliotekanauki.pl/articles/872583.pdf
Data publikacji:
1979
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
Proteus morgani
bakterie
mieso ryb
ryby
histamina
zawartosc histaminy
oznaczanie zawartosci
Źródło:
Roczniki Państwowego Zakładu Higieny; 1979, 30, 4
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł

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