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Tytuł:
Inheritance of Potato virus Y tolerance introgressed from Nicotiana africana to cultivated tobacco
Autorzy:
Korbecka-Glinka, Grażyna
Czubacka, Anna
Depta, Anna
Doroszewska, Teresa
Powiązania:
https://bibliotekanauki.pl/articles/2148716.pdf
Data publikacji:
2017-06
Wydawca:
Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy
Tematy:
Potato virus Y
PVY tolerance
Nicotiana tabacum
Nicotiana africana
Opis:
Potato virus Y (PVY) is an important pathogen of to- bacco. Growing resistant cultivars is the best way to prevent si- gnificant losses of the crop caused by this virus. However, the protection given by the commonly used resistance factor, called va gene, can be overcome by the highly virulent PVY strains. Therefore, tobacco breeding for resistance will benefit from intro- ducing additional PVY resistance/tolerance factors. BPA is a to- bacco breeding line with introgressed PVY tolerance from a wild species N. africana. This trait is effective against a wide range of PVY isolates, including the ones that overcome va resistance. Here, we describe the inheritance of PVY tolerance of BPA. We obtained F1 and F2 plants from reciprocal crosses between BPA and a susceptible tobacco cultivar BP-210. Then we performed me- chanical inoculation tests using sap from PVY infected leaves on both generations of plants. Four weeks later we recorded disease symptoms and subjected all experimental plants to DAS-ELISA tests. All F1 plants developed vein necrosis which confirmed their susceptibility to the virus. The proportion of susceptible and tole- rant plants in the F2 fitted 3:1 ratio which was expected under the assumption that the tolerance is determined by a single, recessive gene. Moreover, the proportion of the susceptible and tolerant in- dividuals did not differ between two F2 populations derived from crosses where BPA was used as a maternal plant or a pollen do- nor, hence cytoplasmic factors do not influence the tolerance of that breeding line.
Źródło:
Polish Journal of Agronomy; 2017, 31; 39-44
2081-2787
Pojawia się w:
Polish Journal of Agronomy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Compatibility and tentoxin-sensivity in the offspring of the near-amphihaploid hybrid Nicotiana tabacum x N.alata
Autorzy:
Berbec, A
Laskowska, D
Powiązania:
https://bibliotekanauki.pl/articles/2046821.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
self-compatibility
chromosome
tentoxin
Nicotiana alata
interspecific hybrid
progeny
Nicotiana tabacum
hybrid plant
cytoplasmic inheritance
Opis:
Near-amphihaploid hybrid plants of Nicotiana tabacwn x N. alata disomic for some N. alata chromosomes were nearly completely self- and cross-sterile but under open pollination conditions occasionally set poorly filled seed capsules containing very small numbers of seeds. Six plants from those seeds which survived to maturity resembled N. alata. Upon cytological examinations five plants were found to have the diploid chromosome complement of N. alata (2n=18), which suggested their androgenetic origin following outcrossing with paternal N. alata. The sixth plant had 9 bivalents and 11 univalents, which was indicative of its hybrid origin. Unlike the self-incompatible N. alata parent, N. alata-like hybrid derivatives were self-compatible. Surprisingly, the selfed progeny of the 18-chromosome plants showed sensitivity to tentoxin, a chloroplast trait characteristic of paternal N. alata but hardly compatible with its cytoplasmic lineage since the tentoxin-insensitive N. tabacum was the cytoplasmic parent of the hybrid. The progeny of the 29-chromosome plant was tentoxin-insensitive, which agreed with the parentage of the hybrid. Populations derived from the 18-chromosome plants were highly uniform but differed sharply from one another. The offspring of the 29-plant was highly heterogeneous mainly because of univalent segregation in that progeny.
Źródło:
Journal of Applied Genetics; 1997, 38, 2
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Diversity of Nicotiana species
Autorzy:
Depta, Anna
Doroszewska, Teresa
Powiązania:
https://bibliotekanauki.pl/articles/37242111.pdf
Data publikacji:
2023
Wydawca:
Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy
Tematy:
Nicotiana
tobacco
morphological diversity
genetic variation
collection
Opis:
The genus Nicotiana is one of the largest in the Solanaceae family and includes more than 80 species. The most well-known and widespread species of the genus Nicotiana is tobacco (Nicotiana tabacum), within which there are numerous cultivars. Tobacco is one of the most important industrial plants in Poland and worldwide. The great diversity within the genus makes it an excellent source of variation in a narrowing gene pool and can be used in breeding programmes. Studies of Nicotiana species also concern mechanisms of polyploidisation and evolution. There are also model species within the genus. However, in order to make full use of the collected germplasm resources, a detailed knowledge of the collection materials is necessary. While there are various reports in the international literature describing specific issues, the aim of this paper is to indicate the diversity of species in the genus Nicotiana as a whole on the basis of our own research and available studies. This review covers the characterisation of the genus Nicotiana in terms of origin and geographical distribution, as well as cytogenetic and molecular differences between species. An important aspect is the presentation of the morphological diversity of Nicotiana accessions and the variation in the most important tobacco alkaloids. A very important issue is the resistance of Nicotiana species to bacterial, fungal and viral diseases, which allows their use in resistance breeding.
Źródło:
Polish Journal of Agronomy; 2023, 52; 123-135
2081-2787
Pojawia się w:
Polish Journal of Agronomy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of selenium on the growth and photosynthetic characteristics of flue-cured tobacco (Nicotiana tabacum L.)
Autorzy:
Jiang, C.
Zu, C.
Shen, J.
Shao, F.
Li, T.
Powiązania:
https://bibliotekanauki.pl/articles/57536.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
selenium
growth
photosynthesis
tobacco plant
Nicotiana tabacum
carboxylation efficiency
Opis:
The objective of this study was to investigate the effect of Selenium (Se) supply (0, 3, 6, 12, 24 mg kg−1) on the growth, photosynthetic characteristics, Se accumulation and distribution of flue-cured tobacco (Nicotiana tabacum L.). Results showed that low-dose Se treatments (≤6 mg kg−1) stimulated plant growth but high-dose Se treatments (≥12 mg kg−1) hindered plant growth. Optimal Se dose (6 mg kg−1) stimulated plant growth by reducing MDA content and improving photosynthetic capability. However, excess Se (24 mg kg−1) increased MDA content by 28%, decreased net photosynthetic rate and carboxylation efficiency by 34% and 39%, respectively. The Se concentration in the roots, stems, and leaves of the tobacco plants significantly increased with increasing Se application. A linear correlation (R = 0.95, P < 0.01) was observed between Se level and tobacco plant tissue Se concentration. This correlation indicated that the tobacco plant tissues were not saturated within the concentration range tested. The pattern of total Se concentration in the tobacco plant tissues followed the order root > leaf > stem. The Se concentration in the roots was 3.17 and 7.57 times higher than that in the leaves and stems, respectively, after treatment with 24 mg kg−1 Se. In conclusion, the present study suggested that optimal Se dose (6 mg kg−1) improved the plant growth mainly by enhancing photosynthesis, stomatal conductance, carboxylation efficiency and Rubisco content in the flue-cured tobacco leaves. However, the inhibition of excess Se on tobacco growth might be due to high accumulation of Se in roots and the damage of photosynthesis in leaves.
Źródło:
Acta Societatis Botanicorum Poloniae; 2015, 84, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana
Autorzy:
Wydro, Mateusz
Kozubek, Edward
Lehmann, Przemysław
Powiązania:
https://bibliotekanauki.pl/articles/1041239.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
RNA silencing
Nicotiana benthamiana
viral suppressor
Agrobacterium-mediated transient expression
Opis:
Here we report on a simple and reproducible system of Agrobacterium-mediated transient gene expression assay that utilizes infiltration of young Nicotiana benthamiana leaves. Although some of the phenomena described in this paper have been already reported by other researchers, here we have further developed them. The highest level of transient gfp gene expression was detected in the youngest leaves of N. benthamiana infiltrated with A. tumefaciens strains AGL0 and EHA105 precultured in the presence of 450-600 µM acetosyringone. Although the maximum level of transient gfp gene expression was restricted presumably by RNA silencing, it was completely suppressed in the presence of the viral protein HC-Pro. The transient expression system described here can be used to identify new viral suppressors of RNA silencing, for detailed analysis of unidentified genes and for industrial production of proteins in plants as well.
Źródło:
Acta Biochimica Polonica; 2006, 53, 2; 289-298
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Green tobacco sickness – A brief review
Autorzy:
Reddy, Mayur Nath T.
Ashok, Navami
Powiązania:
https://bibliotekanauki.pl/articles/1076727.pdf
Data publikacji:
2019
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Green tobacco sickness
Nicotiana tabacum
health hazards
nicotine
occupational exposure
tobacco
Opis:
Green Tobacco Sickness (GTS) has been one of the unexplored areas of occupational health safety. Green Tobacco Sickness is caused by the absorption of nicotine through the skin from wet tobacco plants who have direct contact with tobacco plants during cultivation and harvesting. The present review was carried out to discuss the etiology, symptoms, diagnosis, treatment and prevention of GTS. It is caused by the absorption of nicotine through the skin while the workers are engaged in handling the uncured tobacco leaves. The symptoms include nausea, vomiting, pallor, dizziness, headaches, increased perspiration, chills, abdominal pain, diarrhea, increased salivation, prostration, weakness, breathlessness, and occasional lowering of blood pressure. The use of personal protective equipment like water‑resistant clothing, chemical‑resistant gloves, plastic aprons, and rain‑suits with boots should be used by the tobacco farmers to prevent its occurrence. An international‑level awareness campaign has to be taken up and more stringent workers safety regulations have to be formulated.
Źródło:
World News of Natural Sciences; 2019, 24; 209-214
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Changes accompanying proliferative capacity and morphology of Nicotiana tabacum L. callus in response to 2,4-D
Zmiany związane ze zdolnością do proliferacji i morfologią kalusa Nicotiana tabacum L. w odpowiedzi na różne stężenia 2,4-D
Autorzy:
Gatz, Andrzej
Tomaszewska-Sowa, Agnieszka
Figas, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1628038.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Nicotiana tabacum
2
4-dichlorophenoxyacetic acid
proliferation
cell divisions
callus morphology
Opis:
The common trait of all auxins is a stimulation of cell elongation and also cell division in the presence of cytokinin; both are essential for callus induction and its multiplication. The response of plant tissues to various compounds with auxin activity may be quite different. In this study, the effectiveness of a synthetic auxin, 2,4-dichlorofenoxyacetic acid (2,4-D), instead of the generally applied natural auxin, indole-3-acetic acid (IAA), was tested for the proliferation of Nicotiana tabacum callus. The following concentrations of 2,4-D were tested: 0.1, 0.5, 1.0, 1.5, and 2.0 mg dm−3. Callus was derived from stem pith and its proliferation allowed on MS medium through five subcultures at 25°C and in darkness. After each passage, the fresh weight and morphological features of the callus were determined. The 0.5 mg dm−3 2,4-D treatment was the most favorable for producing the greatest increase in fresh weight in each of five subsequent subcultures as well as maintaining normal morphological features for proliferation. However, the 1.0 mg dm−3 2,4-D treatment in comparison with the lowest, 0.1 mg dm−3, was more beneficial when considering regular increases of fresh weight and a better cell cohesion for callus growth.
Do indukcji i namnażania kalusa niezbędne jest zastosowanie odpowiedniej auksyny, podanej samodzielnie lub z cytokininą. Spośród naturalnych auksyn IAA jest często wykorzystywana do stymulowania proliferacji kalusa Nicotiana tabacum. Zastosowanie IAA wymaga wyższych stężeń ze względu na nietrwałość, wynikającą z wrażliwości na światło i wysoką temperaturę. Takich ograniczeń nie posiada syntetyczna auksyna 2,4-D. Celem tej pracy była ocena przydatności 2,4-D do proliferacji kalusa Nicotiana tabacum z następującymi wariantami stężeń 0.1, 0.5, 1.0, 1.5 i 2 mg dm−3. Proliferację kalusa z rdzenia łodygi przeprowadzono na pożywce MS przez 5 pasaży bez dostępu światła i w temperaturze 25°C. Po każdym pasażu określono świeżą masę i cechy morfologiczne kalusa takie jak barwa, struktura powierzchni i stopień spójności komórek. Spośród badanych stężeń 2,4-D, najefektywniej stymulującym było 0.5 mg dm−3 ze względu na najwyższy przyrost świeżej masy w każdym z pięciu kolejnych pasaży oraz sprzyjające proliferacji cechy morfologiczne kalusa. Wariant stężenia 1.0 mg dm−3 2,4-D w porównaniu z 0.1 mg dm−3 okazał się korzystniejszy, ponieważ charakteryzował się regularnymi przyrostami i lepszą dla namnażania spójnością komórek kalusa.
Źródło:
Acta Agrobotanica; 2017, 70, 4
0065-0951
2300-357X
Pojawia się w:
Acta Agrobotanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of bacterial populations, temperature and exogenous hydrogen peroxide on the induction of the hypersensitive response in Nicotiana tabacum against Xanthomonas perforans
Autorzy:
Farahani, A.S.
Taghavi, S.M.
Powiązania:
https://bibliotekanauki.pl/articles/65481.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bacteria population
temperature
catalase
hydrogen peroxide
hypersensitive response
Nicotiana tabacum
Xanthomonas perforans
Opis:
The objective of this study was to investigate the effects of inoculum concentration, plant post-inoculation incubation temperature and exogenous hydrogen peroxide (H2O2) on the induction of the hypersensitive response (HR) in Nicotiana tabacum against Xanthomonas perforans. Inoculation of leaves with X. perforans at a concentration of 108 CFU · ml–1 and incubation of plants at 30°C resulted in the strongest HR elicitation. Furthermore, an exogenous supply of H2O2 accelerated X. perforans-induced HR, whereas in planta H2O2 removal by application of catalase led to a delay in HR development. Our data suggest that H2O2 has an important role in HR of N. tabacum against X. perforans.
Źródło:
Journal of Plant Protection Research; 2017, 57, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Charakterystyka roznorodnosci w rodzaju Nicotiana na przykladzie pobierania i kumulowania kadmu przez dzikie gatunki
Autorzy:
Doroszewska, T
Powiązania:
https://bibliotekanauki.pl/articles/808119.pdf
Data publikacji:
1998
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
gatunki dzikie
hodowla roslin
pobieranie skladnikow mineralnych
rosliny specjalne
kumulacja
kadm
Nicotiana
tyton
Opis:
Przeprowadzono badania zdolności pobierania i gromadzenia kadmu przez 59 dzikich gatunków Nicotiana i 6 odmian botanicznych N. tabcum i N. rustica. Badania prowadzono na glebie o naturalnej zawartości kadmu 0,15 ppm i glebie z dodawanym kadmem w ilości 8 ppm. W wyniku analizy chemicznej stwierdzono zróżnicowaną zawartość kadmu w liściach i korzeniach w zależności od badanych genotypów. Zdecydowanie największymi zdolnościami do gromadzenia szkodliwego pierwiastka w liściach z podłoża o naturalnej jego zawartości odznaczały się gatunki: N. raimondii, N. bigelovii i odmiany botaniczne N. tabacum, natomiast w korzeniach N. rosulata. W przypadku podłoża, do którego dodano 8 mg czystego składnika najwyższą zawartość kadmu w liściach stwierdzono u N. langsdorfii, N. plumbaginifolia, N. setchelii, N. debneyi i N. velutina. Zawartość kadmu w korzeniach w tej kombinacji najwyższa była u N. miersii, N. clevelandii i N. nudicaulis. Zdecydowanie najmniej kadmu pobierały w obydwu badanych kombinacjach: N. benavidesii i N. umbratica. Różnice istotnie statystycznie wyliczone dla poszczególnych sekcji wykazano najczęściej w kombinacjach z dodawanym kadmem, rzadko w obiektach kontrolnych. Nie wykazano istotnych różnic zawartości kadmu w przypadku odmian botanicznych tego samego gatunku.
Fifty nine wild species of Nicotiana and six botanical varieties of N. tabacum and N.rustica were examined for their ability to take up and accumulate cadmium in their leaves and roots. The plants were grown on two soils: cadmium-enriched (8 ppm Cd), and untreated, with native cadmium content of 0.15 ppm as control. The leaf and root Cd content varied with the genotype and with the level of soil Cd. N. raimondii, N. bigelovii, botanical varieties of N tabacum and N. rosulata were found to be the most potent cadmium absorbers from the untreated soil. N. langsdorfii, N. plumbaginifolia, N. setchelii, N. debneyi and N. velutina took up and accumulated in their leaves large amount of cadmium from the cadmium-enriched soil. The root accumulation of cadmium from cadmium-enriched soil was high in N. miersii, N. clevelandii, and N nudicaulis. N. benavidesii and N. umbratica were found to be the weakest cadmium absorbers from both, cadmium-enriched and untreated soils. In individual sections statistically significant differences were most frequently demonstrated for the treatments with cadmium supplement and rarely for untreated-controls. No significant differences in cadmium content were found among botanical varieties within the same species.
Źródło:
Zeszyty Problemowe Postępów Nauk Rolniczych; 1998, 463; 337-356
0084-5477
Pojawia się w:
Zeszyty Problemowe Postępów Nauk Rolniczych
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Androgenetic haploid plants by culturing anthers of alloplasmic strains of tobacco Nicotiana tabacum L. cv. Zamojska 4 with the cytoplasm of N.knightiana Goodspeed and N.raimondii Macbride
Adrogeniczne rosliny haploidalne z kultur pylnikowych alloplazmatycznych linii tytoniu Nicotiana tabacum L. cv. Zamojska 4 z cytoplazma gatunkow N.knightiana Goodspeed i N.raimondii Macbride
Autorzy:
Berbec, A
Laskowska, D.
Powiązania:
https://bibliotekanauki.pl/articles/68721.pdf
Data publikacji:
1994
Wydawca:
Polska Akademia Nauk. Instytut Genetyki Roślin PAN
Tematy:
hodowla roslin
Nicotiana tabacum
mieszance alloplazmatyczne
tyton uprawny
genetyka roslin
haploidy
haploidy androgeniczne
Źródło:
Genetica Polonica; 1994, 35, 1-2; 33-41
0016-6715
Pojawia się w:
Genetica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Tapetum development in transgenic tobacco [Nicotiana tabacum L.] plants with modified level of histone H1 variants
Autorzy:
Slusarczyk, J
Tykarska, T.
Wierzbicki, A.
Jerzmanowski, A.
Kuras, M.
Powiązania:
https://bibliotekanauki.pl/articles/58139.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
tapetum
development
transgenic plant
tobacco
Nicotiana tabacum
histone level
H1 variant
sterile pollen grain
Opis:
The phenomenon of male sterility has often been observed in investigations on the role of histone H1 in regulation of morphogenetic and cytological processes in transgenic tobacco plants. These changes were accumulated by disturbances in flower development, consisting in lengthening of the pistil style in relation to stamen heads. This prevented pollination and production of seeds. As similar abnormalities occurred also in the present investigations (depending on combination, the sterility% was 84.4 to 19.9, at only 8.1 in the control), the main problem of our investigations was an attempt to explain their reasons. It is commonly known that one of the conditions for formation of fertile pollen is the properly functioning tapetum. Here, we carried out observations of ultrastructure of anther tapetum control cells in respect of abnormalities which occurred during microsporogenesis of transgenic plants with inactivated expression of two major (A, B) and two minor (C, D) histone H1 variants. The investigations were carried out on the following groups of plants: (1) control group with a full set of histone variants (K), (2) with inactivated A and B variants (-AB); (3) with inactivated A, B, C and D variants (-ABCD), (4) with inactivated C and D variants (-CD). It was found that tapetal development was normal in all the investigated groups of plants, and the sequence of changes was similar as in the control. However, certain ultrastructural differences appeared when tapetum functioned as secretory tissue, and in the degeneration phase. In tapetal cell cytoplasm, with participation of rER, lipid bodies were formed, which, having penetrated to the cell surface and to locules, took part in formation of pollen grain sporoderm. Both in the control and in the remaining combination, excluding -ABCD, these bodies looked similar: they were grey, homogenous and surrounded by black jagged deposits. In -ABCD plants, these bodies were more translucent, slightly rarefied, and not surrounded by the deposits. Moreover, in -CD plants, large lipid deposits were frequently observed between remainders of degraded tapetal cells. They did not occur in the control and the remaining combinations.
Źródło:
Acta Societatis Botanicorum Poloniae; 2006, 75, 2; 95-105
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mikrosporogenesis in two alloplasmic isonuclear strains of tobacco Nicotiana tabacum L. cv. Zamojska 4 with the cytoplasms of N.knightiana Goodspeed and N.raimondii Macbride
Mikrosoporogeneza u dwoch alloplazmatycznych izonuklearnych linii tytoniu uprawnego Nicotiana tabacum L. z cytoplazma gatunkow N.knightiana Goodspeed i N.raimondii Macbride
Autorzy:
Berbec, A
Powiązania:
https://bibliotekanauki.pl/articles/67998.pdf
Data publikacji:
1994
Wydawca:
Polska Akademia Nauk. Instytut Genetyki Roślin PAN
Tematy:
sterylnosc meska
Nicotiana tabacum
mieszance alloplazmatyczne
tyton uprawny
mikrosporogeneza
genetyka roslin
mejoza
cytoplazmatyczna meska sterylnosc
Źródło:
Genetica Polonica; 1994, 35, 1-2; 23-31
0016-6715
Pojawia się w:
Genetica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Kolekcja alloplazmatycznych cytoplazmatycznie meskojalowych form tytoniu uprawnego Nicotiana tabacum
Autorzy:
Berbec, A
Powiązania:
https://bibliotekanauki.pl/articles/801204.pdf
Data publikacji:
1998
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
hodowla roslin
Nicotiana tabacum
mieszance alloplazmatyczne
tyton uprawny
cytoplazmatyczna meska sterylnosc
kolekcje odmian
rosliny uzytkowe
Opis:
Cytoplazmę szeregu gatunków rodzaju Nicotiana - N. amplexicaulis, N. bigelovii, N. debneyi, N. eastii, N. exigua, N. glauca, N. glutinosa, N. goodspeedii, N. knightiana, N. occidentalis, N. plumbaginifolia, N. raimondii, N. suaveolens, N. undulata - wbudowano poprzez serię krzyżowań wypierających do genomu uprawnego tytoniu N. tabacum - odmiany Zamojska 4. Kolekcja tak uzyskanych alloplazmatycznych analogów obejmuje także formę ze zmutowaną cytoplazmą N. tabacum. Niektóre z form alloplazmatycznych uzyskano w IUNG (N. amplexicaulis, N. eastii, N. exigua, N. glauca, N. knightiana, N. raimondii, N. tabacum), a pozostałe otrzymano z innych jednostek badawczych. U większości form reakcja na chloroplastowy marker tentoksynę była zgodna z reakcją gatunku dawcy cytoplazmy. Wszystkie rodzaje obcej cytoplazmy (z wyjątkiem cytoplazmy N. knightiana) wywołały efekt pełnej męskiej jałowości u alloplazmatycznych analogów odmiany Zamojska 4. Stopień modyfikacji organów męskich obejmował zakres - od całkowitego braku pręcików u form cms suaveolens, cms tabacum, cms glauca, poprzez pręciki zniekształcone i szczątkowe, występowanie struktur stygmatoidalnych i petaloidalnych, aż do pręcików pozornie normalnych u cms knightiana i cms raimondii. U niektórych form alloplazmatycznych obserwowano również modyfikacje słupka, polegające głównie na występowaniu wielokrotnych znamion oraz niekiedy na skróceniu i staśmieniu całych słupków. Wśród form alloplazmatycznych obserwowano również pewne zróżnicowanie pokroju roślin. U kilku z nich wystąpiła zwiększona podatność na mączniaka rzekomego tytoniu.
Cytoplasms of several Nicotiana species - N. amplexicaulis, N. bigelovii, N. debneyi, N. eastii, N. exigua, N. glauca, N. glutinosa, N. goodspeedii, N. knightiana, N. occidentals, N. plumbaginifolia, N. raimondii, N. suaveolens, N. undulata - were bred into the N. tabacum genomic background of flue cured tobacco Zamojska 4 cv. The collection includes also a cms analogue of Zamojska 4 cv. with, N. tabacum cytoplasm mutant. Some of the alloplasmics were originally obtained in this laboratory (TV. amplexicaulis, N. eastii, N. exigua, N. glauca, N. knightiana, N. raimondii). The remaining ones were acquired from other laboratories and backcrossed into Zamojska 4. Most alloplasmics showed response to tentoxin compatible with that of the cytoplasm donor. All alien cytoplasms except that of N. knightiana produced full male sterility in Zamojska 4. The extent of male organ modifications varied from complete absence of stamens (N. suaveolens, N. tabacum) throught petaloid and stigmatoid structures (most common effect), malformed stamens (N. amplexicaulis, N. glauca) up to apparently normal stamens (N. raimondii, N. knightiana). Pistils were also affected in some of the alloplasmics, multiple stigmas, shortening and fasciation being the most frequent alterations. Some variation in growth and morphology was observed among the alloplasmic analogues of Zamojska 4. Some of the alloplasmics showed increased susceptibility to blue mold.
Źródło:
Zeszyty Problemowe Postępów Nauk Rolniczych; 1998, 463; 51-61
0084-5477
Pojawia się w:
Zeszyty Problemowe Postępów Nauk Rolniczych
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Glutathione boost with ectopic expression of bifunctional STGCL-GS enzyme in tobacco enhances host and non-host defense
Autorzy:
Curcin, S.
Peskan-Berghoefer, T.
Gromes, R.
Rausch, T.
Powiązania:
https://bibliotekanauki.pl/articles/80394.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
glutathione
ectopic expression
plant immunity
pathogen
Nicotiana tabacum
pathogenesis-related protein
hypersensitive response
Pseudomonas syringae
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of change in the proportion of H1 histone variants on microsporogenesis and development of male gametophyte in transgenic plants of tobacco [Nicotiana tabacum L.]
Autorzy:
Slusarczyk, J
Wierzbicki, A.
Przewloka, M.
Tykarska, T.
Jerzmanowski, A.
Kuras, M.
Powiązania:
https://bibliotekanauki.pl/articles/57064.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
sterile pollen grain
male
development
transgenic plant
gametophyte
tobacco
microsporogenesis disorder
histone H1
Nicotiana tabacum
Opis:
As continuation of investigations in to the mechanism of the role of the H1 histone, which is a crucial protein component chromosomes of all eukaryotes, transgenic tobacco plants with different levels of the H1 histone variants were examined. Tobacco has six sequential variants of the H1 histone: two major ones (H1A and H1B), constituting ca. 90% of all H1, and four minor ones (H1C, H1D, H1E and H1F), occurring in very small quantities. The following groups of plants were examined: K - control group with a full set of histone variants; -AB -with the A and B variants removed; -ABCD - with the A, B, C and D variants removed; and -CD - with the C and D variants removed. The analysis of microsporogenesis in those plants, based on preparations squeezed in acetoorcein, revealed the asynchronous course of meiosis in -AB and -ABCD plants, occurrence of chromosomal aberration, and, consequently, the formation of sterile pollen grains (accordingly: 84,4% and 81,4%). In -CD plants, the percentage of aberration and sterile pollen grains was similar to the control material. Electron microscope observations of microsporogenesis showed ultrastructural changes. In -AB and -ABCD plants, a major portion of the pollen grains were degraded. The smallest number of degraded pollen grains, in comparison with the control, was found in the -CD group.
Źródło:
Acta Societatis Botanicorum Poloniae; 2003, 72, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
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