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Tytuł:
The number of E. coli and C. perfringens bacteria in poultry waste and subsequent phases of composting
Liczebność bakterii E. coli i C. perfringens w odpadach drobiarskich oraz kolejnych fazach ich kompostowania
Autorzy:
Cybulska, K.
Suchecka, N.
Wrońska, I.
Mahdi-Oraibi, S.
Powiązania:
https://bibliotekanauki.pl/articles/125776.pdf
Data publikacji:
2015
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
E.coli
C. perfringens
poultry waste
composting
E. coli
odpady drobiarskie
kompostowanie
Opis:
Rapidly growing commercial poultry production generates large amounts of waste. Waste that accumulates during the poultry slaughter process often remains unprocessed, becoming a serious threat to people’s health and the natural environment. Poultry production waste constitutes problems odour threat and dangerous sanitary threat. The aim of this study was to determine the population size of Escherichia coli and Clostridium perfringens in poultry waste and in successive stages of waste composting. Research material consisted of raw feathers collected immediately after the slaughter, samples of biological sludge from the centrifuge, mixture of straw, feathers and lime and processed compost. Microbiological analyses were conducted with the use of spread plate count method and the substrate was used in accordance with research standards. The size of population of microorganisms in the samples analyzed corresponded to the waste processing stage and group of microbes. The presence of C. perfringens strains was ascertained in all samples, whereas E. coli strains were identified only in raw feathers and centrifuge sludge, being most numerous in unprocessed material. A reverse trend was observed in case of C. perfringens with the highest population density in centrifuge sludge and least density in raw feathers.
Intensywnie rozwijająca się produkcja drobiarska wiąże się z generowaniem dużej ilości odpadów. Powstające produkty uboczne podczas uboju pozostawione w stanie surowym stają się poważnym zagrożeniem dla środowiska naturalnego i zdrowia ludzi. Mogą stwarzać problemy odorotwórcze oraz istotnie groźne zanieczyszczenie sanitarne. Celem przeprowadzonych badań było określenie liczebności E. coli i C. perfringens w odpadach drobiarskich oraz kolejnych etapach ich kompostowania. Materiał badawczy stanowiły świeże pióra bezpośrednio po uboju, osad biologiczny z wirówki, mieszanina słomy, pierza i wapna oraz kompost przerobiony. Analizy mikrobiologiczne wykonano metodą płytkową, wykorzystując wybiórcze podłoża zgodne z normami. Liczebność mikroorganizmów w badanych próbach zależała od fazy przerobu odpadu i analizowanej grupy drobnoustrojów. Obecność bakterii C. perfringens stwierdzono we wszystkich próbach, natomiast bakterie E. coli tylko w piórach surowych i osadzie z wirówki, przy czym najliczniej występowały w materiale nieprzetworzonym. Odwrotną tendencję zaobserwowano w przypadku C. perfringens, które najliczniej zasiedlały osad z wirówki, natomiast ich mniejszą liczebność stwierdzono w piórach surowych.
Źródło:
Proceedings of ECOpole; 2015, 9, 1; 19-24
1898-617X
2084-4557
Pojawia się w:
Proceedings of ECOpole
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sanitary evaluation of the water from water intake points situated in Gorzow Wielkopolski and neighbouring localities
Ocena sanitarna wody pochodzącej z ujść wodnych na terenie Gorzowa Wielkopolskiego oraz okolic
Autorzy:
Wrońska, I.
Cybulska, K.
Powiązania:
https://bibliotekanauki.pl/articles/388391.pdf
Data publikacji:
2015
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
drinking water
bacteria
E.coli
E. faecalis
water contamination
woda pitna
bakteria
E. coli
zanieczyszczenie wody
Opis:
Water is an essential component necessary for sustaining life. Nevertheless, it may pose a serious threat to human life resulting from possible occurrence of pathogenic and potentially pathogenic microorganisms. Therefore, monitoring and continuous microbial surveillance of the water intended for consumption is extremely important. The requirements referring to qualitative parameters which should be met by water are set out in the Regulation of the Minister of Health of 29 March 2007, as amended on 20 April 2010, and the EU directives. The quality of water being supplied to consumers depends on many factors, among other on the type of water intake (surface water or groundwater), efficiency of water treatment and disinfection, and technical condition of the water supply network. The analysis of the sanitary condition of drinking water has been conducted form water intakes in the city of Gorzow Wielkopolski, ie Centralny Kosynierow Gdynskich intake, Klodawa intake and Siedlice intake, and in neighbouring localities, ie. Maszewo and Marwice. The research material was raw and treated water samples. The population size of Faecal streptococci, coliform bacteria, including E. coli, and total mesophilic and psychrophilic bacteria counts was determined. The analysis of the sanitary condition of drinking water has been conducted. The number of coliform bacteria, including E. coli, and streptococci was determined by the method of membrane filters, whereas total mesophilic and psychrophilic bacteria counts using the plate method under incubation conditions conforming to the standards. The presence of mesophilic and psychrophilic bacteria was observed in all examined water intake points. Differences in their counts over the whole year being significant. In three research points, ie Centralny Kosynierow Gdynskich intake, Maszewo intake and Marwice intake, psychrophilic bacteria count was exceeded (100 CFU ۰ cm–3). In the case of pathogenic bacteria, their occurrence was occasionally observed in the drinking water intake points. The water treatment processes applied and, as the final result, water sanitary assessment according to the existing standards and the provision stipulated allow it to be collected for consumption purposes and do not pose the threat to human health and life.
Woda jest podstawowym składnikiem niezbędnym do utrzymania życia. Jednakże, może ona stworzyć poważne zagrożenie dla zdrowia człowieka wynikające z możliwości wystąpienia mikroorganizmów chorobotwórczych i potencjalnie chorobotwórczych. W związku z tym, niezwykle istotna jest kontrola oraz stały nadzór mikrobiologiczny nad wodą przeznaczoną do spożycia. Wytyczne co do parametrów jakościowych, jakim powinna odpowiadać, zawarte są w Rozporządzeniu Ministra Zdrowia z dnia 29 marca 2007 r. wraz ze zmianą z dnia 20 kwietnia 2010 r. oraz Dyrektywach Unii Europejskiej. Jakość wody dostarczanej konsumentom zależy od wielu czynników m.in.: rodzaju ujęcia (woda powierzchniowa lub podziemna), skuteczności procesów uzdatniania i dezynfekcji oraz stanu technicznego sieci wodociągowej. Przeprowadzono analizę stanu sanitarnego wody pitnej pochodzącej z ujęć wodnych zaopatrujących miasto Gorzów Wielkopolski, tj. ujęcie Centralne Kosynierów Gdyńskich, Kłodawa, Siedlice oraz sąsiadujące miejscowości, tj. Maszewo i Marwice. Materiałem badawczym były próbki wody surowej i uzdatnionej. Określono liczebność paciorkowców kałowych, bakterii z grupy coli, w tym E. coli oraz ogólnej liczebności bakterii mezofilnych i psychrofilnych. Liczebność bakterii z grupy coli, w tym E. coli oraz paciorkowców kałowych oznaczono metodą filtrów membranowych, zaś ogólną liczebność bakterii mezofilnych i psychrofilnych metodą płytkową w warunkach inkubacji zgodnych z normami. We wszystkich badanych punktach stwierdzono obecność bakterii mezofilnych i psychrofilnych. Różnice w ich liczebności na przestrzeni całego roku były istotne. W punktach badawczych, tj. Centralne Kosynierów Gdyńskich, Maszewo oraz Marwice odnotowano przekroczenie norm ilości psychrofilii 100 j.t.k. ۰ cm–3. Obecność bakterii chorobotwórczych w badanych próbkach wody pitnej wykryto sporadycznie. Zastosowane procesy uzdatniania i w efekcie końcowym ocena sanitarna wody według obowiązujących norm i zawartej klauzuli pozwalają na jej pobór do spożycia i nie stanowią zagrożenia dla zdrowia i życia ludzi.
Źródło:
Ecological Chemistry and Engineering. A; 2015, 22, 1; 17-26
1898-6188
2084-4530
Pojawia się w:
Ecological Chemistry and Engineering. A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Escherichia coli in sewage sludge - detection method
Escherichia coli w osadach ściekowych - metoda wykrywania
Autorzy:
Machnicka, A.
Powiązania:
https://bibliotekanauki.pl/articles/106338.pdf
Data publikacji:
2014
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
E.coli
sewage sludge
enrichment of Escherichia coli
detection of Escherichia coli
E. coli
osady ściekowe
namnażanie Escherichia coli
wykrywanie Escherichia coli
Opis:
Escherichia coli is Gram-negative optionally anaerobic roads which belongs to Enterobacteriaceae family. Includes in a physiological bacterial flora of human and warm-blooded animals large intestine. Escherichia coli is being met in abiotic elements of the environment so as waters, wastewater, sewage sludge, soil and the food. This bacterium is showing the pathogenicity in named terms for the peoples, triggering diseases mainly: gastrointestinal tract and urinary. Quality and quantitative proposed detections method of the bacteria E. coli contains five/six steps: - appointment dry suspended solid, - preparation averaged, test of sample and resuscitation of bacteria, - making dilutions, - enrichment and differentiation in chromogenic-selective medium, - enumerating the amount of cfu E. coli in 1 g of a dry weight, - optionally, the biochemical identification.
Escherichia coli jest Gram-negatywną, względnie beztlenową pałeczką należącą do rodziny Enterobacteriaceae. Wchodzi w skład fizjologicznej flory bakteryjnej jelita grubego człowieka oraz zwierząt stałocieplnych. Spotyka się ją w elementach abiotycznych środowiska, takich jak wody, ścieki, osady ściekowe, gleba i żywność. Bakteria ta w określonych warunkach wykazuje chorobotwórczość dla człowieka, wywołując głównie schorzenia układów pokarmowego i moczowego. Proponowana metoda jakościowa i ilościowa wykrywania bakterii E. coli zawiera pięć/sześć etapów: - oznaczenie suchej masy osadu, - przygotowanie próbki uśrednionej i badawczej oraz przywrócenie bakteriom aktywności fizjologicznej, - wykonanie rozcieńczeń, - namnażanie i identyfikacja na podłożu chromogennym-selektywnym, - wyliczenie ilości jtk E. coli w 1 g suchej masy osadu, - opcjonalnie identyfikacja biochemiczna.
Źródło:
Chemistry-Didactics-Ecology-Metrology; 2014, 19, 1-2; 79-85
2084-4506
Pojawia się w:
Chemistry-Didactics-Ecology-Metrology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Maillard neoglycans as inhibitors for in vitro adhesion of F4+ enterotoxigenic Escherichia coli to piglet intestinal cells
Autorzy:
Sarabia-Sainz, Héctor
Mata Haro, Verónica
Sarabia Sainz, José
Vázquez-Moreno, Luz
Montfort, Gabriela
Powiązania:
https://bibliotekanauki.pl/articles/1038559.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Albumin glycation
biorecognition
anti-adhesion
E. coli
Opis:
Adhesion of enterotoxigenic (ETEC) E. coli to host intestinal cells is mediated by lectin-like fimbriae that bind to specific glycan moieties on the surfaces of enterocytes. To prevent in vitro binding of E. coli F4 fimbriae (F4 ETEC+) to piglet enterocytes, neoglycans were synthesized by the Maillard reaction conjugating lactose (Lac), galacto-oligosaccharides (GOS) or chitin oligosaccharides (Ochit) to porcine serum albumin (PSA). Neoglycans were characterized by SDS-PAGE, intrinsic tryptophan fluorescence and recognition by plant lectins, as well as by F4 ETEC variants. Electrophoretic patterns suggested the binding to PSA of 63, 13 and 2 molecules of Lac, GOS and Ochit, respectively. All neoglycans displayed quenching of tryptophan fluorescence consistent with the degree of glycation estimated by SDS-PAGE. Plant lectins recognized the neoglycans according to their specificity, whereas antigenic variants of F4 ETEC (ab, ac and ad) recognized PSA-Ochit and PSA-Lac with higher affinity than that for GOS. Neoglycans partially hindered the in vitro binding of F4+ ETEC to piglet enterocytes in a dose-dependent manner. The most effective blocking was observed with PSA-Lac that partially inhibited the adhesion of bacteria to enterocytes in a dose dependent manner, as quantified by flow cytometry. Increased production of the cytokines IL-6 and TNF-α was observed in response to F4+ ETEC infection of enterocytes and production was reduced in the presence of PSA-Ochit and PSA-GOS. These results suggest that neoglycans synthesized by the Maillard reaction could be useful in the prophylaxis of diarrhea in piglets.
Źródło:
Acta Biochimica Polonica; 2017, 64, 4; 679-686
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microbiological air rating in a variety of objects during treatment of the post-slaughter poultry wastes Part I. Escherichia coli
Autorzy:
Oraibi, S. M.
Cybulska, K.
Powiązania:
https://bibliotekanauki.pl/articles/125448.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
E.coli
air
poultry waste facilities
composting
Opis:
The presence of Escherichia coli in the air of facilities involved in management and composting of post-slaughter poultry wastes in selected plants of West Western Pomerania region was studied. Measurements were made on four dates in a variety of weather conditions during the year. The study was conducted at 5 objects that differ in the type of waste and the degree of preparation for composting. These were: chemical treatment and preliminary processing plant, liquid wastes reservoir, platform for preparation of materials for composting, storage of biological sediments, and composting facility. Measurement of bacteria count was carried out in accordance with the applicable procedures on selective chromogenic TBX medium. The assays revealed the presence of E. coli at all test objects, but not always on all measurement dates. It has been shown that the presence of E. coli was from 20 to 3047 CFUm-3 of air, although the largest quantities were most frequently detected in the air of the building for post-slaughter waste pre-treatment in chemical treatment plant.
Źródło:
Journal of Ecological Engineering; 2016, 17, 5; 17-21
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Type 1 fimbriae in commensal Escherichia coli derived from healthy humans
Autorzy:
Pusz, Paweł
Bok, Ewa
Mazurek, Justyna
Stosik, Michał
Baldy-Chudzik, Katarzyna
Powiązania:
https://bibliotekanauki.pl/articles/1039310.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
commensal E. coli
type 1 fimbriae
gene expression
Opis:
Type 1 fimbriae are one of the most important factors of Escherichia coli adaptation to different niches in the host. Our study indicated that the genetic marker - fimH gene occurred commonly in commensal E. coli derived from healthy humans but expression of the type 1 fimbriae was not observed. Identification of fim structural subunit genes (fimA-fimH) and recombinase fimE and fimB genes showed that many of the strains were carrying an incomplete set of genes and the genes expression study revealed that in strains with complete set of fim genes, the fimC gene, encoding the chaperone protein, was not expressed.
Źródło:
Acta Biochimica Polonica; 2014, 61, 2; 389-392
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation of bacteria from soil sample of Tamil Nadu and their in vitro interaction
Autorzy:
Sankari, D.
Khusro, Ameer
Aarti, Chirom
Powiązania:
https://bibliotekanauki.pl/articles/1031683.pdf
Data publikacji:
2020
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Antagonism
B. subtilis
CFU
E. coli
Interaction
P. aeruginosa
Opis:
The present study was investigated to determine the competitive interaction between Gram positive (B. subtilis) and Gram negative (P. aeruginosa and E. coli) bacteria in vitro. Bacteria of interest were isolated from soil sample and were identified through morphological and biochemical tests. Bacterial cultures were grown alone and as co- culture in test tubes containing sterile broth. Bacterial growth and their growth inhibition in co- culture tubes were analyzed till 96 h through spectrophotometric assay and colony forming unit (CFU) assay. Spectrophotometric analysis and CFU/mL assay showed that the effect of B. subtilis on P. aeruginosa and E. coli in co- culture tubes was growth inhibitory. This is accompanied by the reduction in absorbance value and CFU/mL of the co-culture tubes. The bactericidal activity of B. subtilis was measured on P. aeruginosa and E. coli using agar well diffusion method. B. subtilis showed zone of inhibition of 8 mm and 6 mm against P. aeruginosa and E. coli, respectively. The antagonistic activity test of B. subtilis favoured the spectrophotometric and colony forming assay results. These results suggest that B. subtilis develop adaptational pathways by extracellular signaling molecules and antibacterial factors in order to compete with bacterial neighbors.
Źródło:
World News of Natural Sciences; 2020, 28; 67-75
2543-5426
Pojawia się w:
World News of Natural Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mutator specificity of Escherichia coli alkB117 allele
Autorzy:
Nieminuszczy, Jadwiga
Janion, Celina
Grzesiuk, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/1041261.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
lacZ → Lac+ reversion
mutational specificity
E. coli
MMS
alkB117
Opis:
The Escherichia coli AlkB protein encoded by alkB gene was recently found to repair cytotoxic DNA lesions 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) by using a novel iron-catalysed oxidative demethylation mechanism that protects the cell from the toxic effects of methylating agents. Mutation in alkB results in increased sensitivity to MMS and elevated level of MMS-induced mutations. The aim of this study was to analyse the mutational specificity of alkB117 in a system developed by J.H. Miller involving two sets of E. coli lacZ mutants, CC101-106 allowing the identification of base pair substitutions, and CC107-CC111 indicating frameshift mutations. Of the six possible base substitutions, the presence of alkB117 allele led to an increased level of GC→AT transitions and GC→TA and AT→TA transversions. After MMS treatment the level of GC→AT transitions increased the most, 22-fold. Among frameshift mutations, the most numerous were -2CG, -1G, and -1A deletions and +1G insertion. MMS treatment appreciably increased all of the above types of frameshifts, with additional appearance of the +1A insertion.
Źródło:
Acta Biochimica Polonica; 2006, 53, 2; 425-428
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
COMPARATIVE IN VITRO STUDIES OF FURAZIDIN AND NITROFURANTOIN ACTIVITIES AGAINST COMMON UROPATHOGENS INCLUDING MULTIDRUG-RESISTANT STRAINS OF E. COLI AND S. AUREUS
Autorzy:
Klesiewicz, Karolina
Karczewska, Elżbieta
Nowak, Paweł
Mrowiec, Paulina M.
Skiba-Kurek, Iwona
Białecka, Joanna
Majka, Zbigniew
Berdzik-Kalarus, Sylwia
Budak, Alicja
Zajdel, Paweł
Powiązania:
https://bibliotekanauki.pl/articles/895689.pdf
Data publikacji:
2018-06-30
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
ESBL-positive E. coli
MRSA
furazidin
nitrofurantoin
urinary tract infections
Opis:
Abstract: Urinary tract infections caused by wide range of pathogens including gram-negative and gram-positive bacteria as well as fungi are a severe public health problem. The predominant causative agent of both uncomplicated and complicated urinary tract infections is Escherichia coli. In an era of increasing bacterial resistance to antimicrobial agents and a high prevalence of multidrug-resistant (MDR) strains in community and hospital acquired infections, the re-evaluation of older generations of antimicrobial agents, such as nitrofuran derivatives, seems to be a reasonable approach. The aim of the study was to evaluate furazidin activity against common uropathogens in comparison to nitrofurantoin and other selected antimicrobial agents, routinely used in the treatment of urinary tract infections. Furazidin exhibited lower MICs than nitrofurantoin when tested against gram-negative and gram-positive bacteria including clinical MDR E. coli and methicillin-resistant Staphylococcus aureus. The MICs for furazidin ranged from 4 to 64 mg/L for Enterobacteriaceae strains, from 2 to 4 mg/L for gram-positive cocci, and 0.5 mg/L for anaerobic bacteria. The MICs for nitrofurantoin ranged from 16 to 64 mg/L for Enterobacteriaceae strains, from 8 to 64 mg/L for gram-positive cocci, and 4 mg/L for anaerobic bacteria. In addition, both nitrofurans displayed better activity against the tested bacterial strains than ciprofloxacin, fosfomycin, trimethoprim and co-trimoxazole. Nitrofuran derivatives displayed higher antimicrobial activity than other antimicrobial agents regardless of bacteria species or resistance mechanism.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 3
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
CEA-negative glioblastoma and melanoma cells are sensitive to cytosine deaminase/5-fluorocytosine therapy directed by the carcinoembryonic antigen promoter.
Autorzy:
Dąbrowska, Anna
Szary, Jarosław
Kowalczuk, Małgorzata
Szala, Stanisław
Ugorski, Maciej
Powiązania:
https://bibliotekanauki.pl/articles/1041550.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
CEA promoter
E. coli cytosine deaminase
gene-directed enzyme prodrug therapy
Opis:
Recent studies have suggested that carcinoembryonic antigen (CEA)-promoter sequences are active only in CEA-positive cells, filing in the criteria for tumor specific targeting of suicide genes. However, the present study on gene therapy of colon cancer and cell-specificity of CEA promoter, provide evidence that CEA-positive and CEA-negative cells transfected with E. coli cytosine deaminase (CD) gene under the control of CEA promotor sequence are sensitive to enzyme/pro-drug therapy with 5-fluorocytosine (5-FC). Individual clones derived from the CEA-negative cell lines: melanoma Hs294T and glioblastoma T98G after transfection with CD differed profoundly in their sensitivity to 5-FC. The IC50 values for several clones of the CEA-negative cells were almost the same as for CEA-positive colon cancer cells. Such 5-FC-sensitive clones derived from the population of CEA-negative cells, present even in small number, because of the very effective bystender effect of this enzyme/pro-drug system can cause severe problems during therapy by efficiently killing surrounding normal cells. Safety is the major issue in gene therapy. Our data suggest that the safety of gene-directed enzyme pro-drug therapy (GDEPT) with CEA promoter driven expression of therapeutic genes is not so obvious as it has originally been claimed.
Źródło:
Acta Biochimica Polonica; 2004, 51, 3; 723-732
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection and enumeration of Enteric bacteria associated with food handlers and surfaces of food manufacturing industry located in Hub city, Pakistan
Autorzy:
Ali, Razim
Hayat, Amir
Fatima, Madiha
Noman, Muhammad
Powiązania:
https://bibliotekanauki.pl/articles/1182928.pdf
Data publikacji:
2016
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
enterobacteriaceae
foodborne microbes
hygiene
surface contamination
food handlers
e. coli
sanitation
Opis:
Foodborne microorganisms harbor and adheres itself to the food material and surrounding surfaces for a long time and influence the food quality and consumers health. Among these microbes the presence of Enteric indicator bacteria in food premises confers the indication of entero-pathogens, i.e. E. coli, Salmonella, Shigella and Campylobacters that could cause severe systemic infections in consumers. In this study, the hygienic status of confectionery and supplementary food processing facility was evaluated. A total of 10497 examinations were performed on 3499 swab samples collected from food premises and handlers for the analysis of Enteric indicator bacteria. From swabs, 1277 (12.2%) isolates were identified in which Enterobacteriaceae were found with higher frequency 604 (47.3%) followed by Coliforms 293 (30.8%) and Escherichia coli 280 (21.9%) respectively. The mean count (CFU/cm2) was found maximum for plain surfaces (floors, walls and door), while the lowest was for equipment and machinery. Overall isolates percent prevalence was determined where Enterobacteriaceae were 47%, Coliforms 31% and Escherichia coli 22%. Majority of the floor surfaces were highly contaminated, where washing and sanitation practices were observed to be inappropriate. Worker hygiene status was lacking essential food safety and hygiene standards. In general, the Enteric bacteria were found with higher ratio, that could affect the food quality and quantity both to a greater extent with some influences on consumers health.
Źródło:
World Scientific News; 2016, 49, 2; 192-203
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Design of small molecule inhibitors of type III secretion system ATPase EscN from enteropathogenic Escherichia coli
Autorzy:
Bzdzion, Lukasz
Krezel, Hanna
Wrzeszcz, Karol
Grzegorek, Irmina
Nowinska, Katarzyna
Chodaczek, Grzegorz
Swietnicki, Wieslaw
Powiązania:
https://bibliotekanauki.pl/articles/1038684.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Escherichia coli (E. coli)
type III secretion system (T3SS)
enzyme inhibitor
small molecule
Opis:
Enteropathogenic E. coli (EPEC) is a human pathogen using type III secretion system for delivery of proteins directly into the human host. The system contains a single ATPase, EscN, which is essential for uncoupling of proteins from their complexes with chaperones before the delivery. The structure of EscN ATPase (PDB code: 2obm) was used to screen computationally for small molecule inhibitors blocking its active site. Two lead candidates were examined but only one, Compound 54, was selected for further optimization. After extended QSAR optimization, two derivatives were found to be competitive inhibitors of EscN capable of blocking ATPase activity with a Ki below 50 µM. One candidate, WEN05-03, with a Ki=16±2 µM, was also minimally toxic to mammalian cells as determined by other assays. In the cell infection model of HeLa cells with EPEC, Compound WEN05-03 completely blocked actin cluster formation at 100 µM concentration, when analyzed by confocal microscopy. The second best inhibitor of EscN ATPase activity was WEN04-34 with a Ki=46±2 µM. However, the compound was highly toxic to the BALB/3T3 cell line. In summary, the work identifies a compound blocking bacterial ATPase in its active site without causing cellular toxicity to the host cells. It is the first report showing feasibility of using bacterial virulence system ATPase as a target for safe, non-toxic compounds and offering a proof-of-concept for non-antibiotic alternatives.
Źródło:
Acta Biochimica Polonica; 2017, 64, 1; 49-63
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of SOS-induced Pol II, Pol IV, and Pol V DNA polymerases on UV-induced mutagenesis and MFD repair in Escherichia coli cells.
Autorzy:
Wrzesiński, Michał
Nowosielska, Anetta
Nieminuszczy, Jadwiga
Grzesiuk, Elżbieta
Powiązania:
https://bibliotekanauki.pl/articles/1041471.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
MFD
polB
UV induced mutagenesis
E. coli
dinB
mutD5
umuDC
Opis:
Irradiation of organisms with UV light produces genotoxic and mutagenic lesions in DNA. Replication through these lesions (translesion DNA synthesis, TSL) in Escherichia coli requires polymerase V (Pol V) and polymerase III (Pol III) holoenzyme. However, some evidence indicates that in the absence of Pol V, and with Pol III inactivated in its proofreading activity by the mutD5 mutation, efficient TSL takes place. The aim of this work was to estimate the involvement of SOS-inducible DNA polymerases, Pol II, Pol IV and Pol V, in UV mutagenesis and in mutation frequency decline (MFD), a mechanism of repair of UV-induced damage to DNA under conditions of arrested protein synthesis. Using the argE3→Arg+ reversion to prototrophy system in E. coli AB1157, we found that the umuDC-encoded Pol V is the only SOS-inducible polymerase required for UV mutagenesis, since in its absence the level of Arg+ revertants is extremely low and independent of Pol II and/or Pol IV. The low level of UV-induced Arg+ revertants observed in the AB1157mutD5ΔumuDC strain indicates that under conditions of disturbed proofreading activity of Pol III and lack of Pol V, UV-induced lesions are bypassed without inducing mutations. The presented results also indicate that Pol V may provide substrates for MFD repair; moreover, we suggest that only those DNA lesions which result from umuDC-directed UV mutagenesis are subject to MFD repair.
Źródło:
Acta Biochimica Polonica; 2005, 52, 1; 139-147
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Using multitype branching models to analyze bacterial pathogenicity
Autorzy:
Tahir, Daniah
Kaj, Ingemar
Bartoszek, Krzysztof
Majchrzak, Marta
Parniewski, Pawel
Sakowski, Sebastian
Powiązania:
https://bibliotekanauki.pl/articles/747904.pdf
Data publikacji:
2020
Wydawca:
Polskie Towarzystwo Matematyczne
Tematy:
Markov models
branching processes
limit theorems
virulence factors
E. coli strains
Opis:
We apply multitype, continuous time, Markov branching models to study pathogenicity in E. coli, a bacterium belonging to the genus Escherichia. First, we examine briefly, the properties of multitype branching processes and we also survey some fundamental limit theorems regarding the behavior of such models under various conditions. These theorems are then applied to discrete, state dependent models, in order to analyze pathogenicity in a published clinical data set consisting of 251 strains of E. coli. We use well established methods, incorporating maximum likelihood techniques, to estimate speciation rates as well as the rates of transition between different states of the models. From the analysis, we not only derive new results, but we also verify some preexisting notions about virulent behavior in bacterial strains.
Źródło:
Mathematica Applicanda; 2020, 48
1730-2668
2299-4009
Pojawia się w:
Mathematica Applicanda
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of microbiological quality of water in the Nowohucki Reservoir with particular regard to microorganisms potentially dangerous to humans
Autorzy:
Wolny-Koładka, Katarzyna
Powiązania:
https://bibliotekanauki.pl/articles/1035482.pdf
Data publikacji:
2016
Wydawca:
Instytut Medycyny Wsi
Tematy:
"E. coli"
"E. faecalis"
"Nowohucki Reservoir"
"mesophilic bacteria"
"microbiological contamination of water"
"surface water"
Opis:
Introduction. This study was aimed to assess the microbiological quality of water in the Nowohucki Reservoir (Kraków, Poland) as well as to determine whether its waters contain microorganisms potentially dangerous from an epidemiological point of view. Material and methods. Microbiological analyses included the determination of the number of mesophilic and psychrophilic bacteria, coliforms, fecal E. coli, as well as E. faecalis, C. perfringens, Staphylococcus spp. and Salmonella spp.. Water samples were collected 4 times per year on April 27th 2015 (spring), July 10th 2015 (summer), October 12th 2015 (autumn) and December 29th 2015 (winter) at 5 points within the area of the reservoir. Water and air temperature was measured onsite. Results. It was found that the prevalence of the analyzed microorganisms was affected by changing water and air temperature as well as by using this reservoir during holiday season for swimming purposes by local residents. All analyzed microbiological indicators of poor water quality were found in the analyzed water samples, which may pose a potential health risk to people swimming in the considered reservoir. Conclusions. From an epidemiological point of view, it is reasonable to include the Nowohucki Reservoir into a constant sanitary monitoring programme.
Wstęp. Badania miały na celu ocenę stanu mikrobiologicznego wód Zalewu w Nowej Hucie (Kraków, Polska) a także określenie, czy w zbiorniku występują drobnoustroje potencjalnie niebezpieczne z epidemiologicznego punktu widzenia. Materiał i metody. Analizy mikrobiologiczne obejmowały określenie liczebności bakterii mezofilnych, psychrofilnych, bakterii grupy coli, form kałowych E. coli, a także E. faecalis, C. perfringens, Staphylococcus spp. i Salmonella spp.. Próbki wód pobierane były 4 razy w roku w następujących terminach: 27.04.2015 r. (wiosna), 10.07.2015 r. (lato), 12.10.2015 r. (jesień) i 29.12.2015 r. (zima) w 5 punktach na terenie zalewu. Ponadto, w każdym punkcie pomiarowym mierzono temperaturę wody oraz powietrza. Wyniki. Stwierdzono, że na liczebność badanych drobnoustrojów wpływała zmieniająca się temperatura wody i powietrza oraz użytkowanie w sezonie wakacyjnym zalewu w charakterze kąpieliska przez okolicznych mieszkańców. W pobranych próbkach zidentyfikowano wszystkie badane wskaźniki czystości mikrobiologicznej wód, które stanowią potencjalne zagrożenie dla kąpiących się w zbiorniku ludzi. Wnioski. Z epidemiologicznego punktu widzenia zasadnym jest objęcie wód Zalewu w Nowej Hucie stałym monitoringiem sanitarnym.
Źródło:
Medycyna Środowiskowa - Environmental Medicine; 2016, 19, 4; 19-26
1505-7054
2084-6312
Pojawia się w:
Medycyna Środowiskowa - Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 31

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