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Tytuł:
Określenie pochodzenia wyłączonych drzewostanów nasiennych sosny rychtalskiej (Pinus sylvestris L.) z wykorzystaniem markerów mikrosatelitarnych
Determination of the origin of the rychtal Scots pine (Pinus sylvestris L.) seed tree stands using microsatellite markers
Autorzy:
Wójkiewicz, B.
Żukowska, W.B.
Urbaniak, L.
Kowalczyk, J.
Litkowiec, M.
Lewandowski, A.
Powiązania:
https://bibliotekanauki.pl/articles/985719.pdf
Data publikacji:
2019
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
hodowla lasu
sosna zwyczajna
Pinus sylvestris
sosna rychtalska
drzewostany nasienne
pochodzenie roslin
zmiennosc genetyczna
analiza DNA
markery mikrosatelitarne
scots pine
genetic variation
gene pool
genetic structure
ssr markers
Opis:
The rychtal pine is one of the most valuable ecotypes of Scots pine (Pinus sylvestris L.) approved for the breeding purposes in Poland. However, it occupies stands typical for oaks and beeches as shown by the compatibility analysis of species composition in relation to the habitat type in which they occur. Such result raises some doubts in terms of the naturalness of the rychtal pine and calls its history and origin into question. In the present study, we used the set of nuclear microsatellite markers to characterize and compare the gene pool composition of the selected seed tree stands of the rychtal pine with 200−year−old pine trees which grow at the Syców Forest District (SW Poland). We aimed to know to what extent the set of alleles specified for the group of the oldest trees from natural habitats is represented in the younger forest tree stands of the rychtal pine. The analysis of molecular variance (AMOVA) and clustering analysis showed that the gene pool of the studied pine populations was homogenous (FST=0,02%, K=1). The parameters of genetic variation were similar for all populations except for the mean number of alleles. On average, 25 new alleles were found in two rychtal pine seed tree stands as compared to the set of alleles found in the group of old pine trees. However, all alleles defined for old pines were also present in the gene pool of younger rychtal pine forest stands. The differences in the gene pool richness result most likely from quite high differences in the number of individuals analyzed from each population. In conclusion, our results indicate the common origin of the studied Scots pine populations.
Źródło:
Sylwan; 2019, 163, 08; 637-644
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Porównanie zmienności genetycznej pokolenia matecznego i sztucznie wyhodowanego potomstwa sosny zwyczajnej na podstawie analiz DNA
Comparison of the genetic variability of Scots pine trees and their progeny from nursery production based on DNA analyses
Autorzy:
Konecka, A.
Tereba, A.
Bieniek, J.
Nowakowska, J.A.
Powiązania:
https://bibliotekanauki.pl/articles/985843.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
hodowla lasu
sosna zwyczajna
Pinus sylvestris
zmiennosc genetyczna
analiza DNA
markery mikrosatelitarne
drzewa mateczne
drzewa potomne
sadzonki z zakrytym systemem korzeniowym
genetic diversity
ssr markers
forest nursery production
pinus sylvestris l.
Opis:
The production of forest tree species in forest nurseries is performed via two main breeding systems: i) the traditional (conventional) way with the seedlings grown in soil, and ii) plants cultivated in the containers. The aim of the study was to assess the level of genetic variability in the populations of the mother stands and the progeny populations of Scots pine cultured with traditional way (in soil) and in containers in two nurseries in Olsztynek (N Poland) and Oleszyce (S Poland) forest districts. Four polymorphic microsatellite markers (SPAG 7.14, SPAC 11.6, SPAC 12.5 and SsrPt_ctg4363) were used to evaluate the genetic variability of the studied populations. The basic hypothesis assumed that higher gene pool characterizes the seedlings grown in the containers comparing to the seedlings grown in the ground. The results confirmed that. Seedlings from containerized breeding had larger gene pool and were more diverse than plants with conventional breeding, both in Olsztynek and Oleszyce. Our study revealed a significant human impact on shaping the pool of forest genetic resources of Polish forests at the early stage of nursery production and showed the need for a broader study on further stages of cultivation of forests.
Źródło:
Sylwan; 2018, 162, 01; 32-40
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Stan zdrowotny a zróżnicowanie genetyczne wybranych drzewostanów świerkowych na terenie RDLP w Krośnie
Genetic variability and health of Norway spruce stands in the Regional Directorate of the State Forests in Krosno
Autorzy:
Gutkowska, J.
Borys, M.
Tereba, A.
Tkaczyk, M.
Oszako, T.
Nowakowska, J.A.
Powiązania:
https://bibliotekanauki.pl/articles/1293265.pdf
Data publikacji:
2017
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
lesnictwo
RDLP Krosno
drzewostany swierkowe
stan zdrowotny lasu
drzewa lesne
swierk pospolity
Picea abies
zroznicowanie genetyczne
markery molekularne
markery mikrosatelitarne
DNA mitochondrialny
Norway spruce
health state
SSR markers
mtDNA
genetic diversity
Opis:
The study was conducted in 2015 in six spruce stands situated in different forest districts administratively belonging to the Regional Directorate of Forests State in Krosno. Each spruce population was represented by 30 trees and assessed in terms of their current health status. Genetic analyses were performed based on shoot samples from each tree using nine nuclear DNA markers and one mitochondrial DNA marker (nad1). The health status of the trees was described according to the classification developed by Szczepkowski and Tarasiuk (2005) and the correlation between health classes and the level of genetic variability was computed with STATISTICA (α = 0.05). Nuclear DNA analyses revealed a low level of genetic variability among spruce populations (only 3% of the total genetic variation (FST = 0.028) and a high variability within populations (97%). The total heterozygosity in all stands (HT) was calculated as 0.646. Based on UPGMA analysis, the most genetically similar populations are spruce stands in the Bieszczady National Park and the Ustrzyki Dolne Forest District, which have the smallest genetic divergence of all populations (DN = 0.0165). Our analysis of the mitochondrial gene nad1 revealed the presence of six different haplotypes “a”, “a1”, “b”, “c”, “d” and “d1”. Comprising 56% of all haplotypes, “a” was the most common showing a predominant impact on spruce migration from the Carpathian area. The analysis based on mitochondrial markers (by Nei) revealed a heterozygosity of 0.525. Based on the observations of disease symptoms, 29% of the trees belong to health class 1,30% to class 2,28% to class 3 and class 4 contains 13% of trees. The comparison between health status and the level of genetic variation in the analyzed stands showed a positive correlation. Spruce stands with better health were also characterized by a greater degree of genetic variability. Since most of the investigated spruce populations shared the mitochondrial haplotype “a”, we have ascertained their Hercynian- Carpathian origin. Only one stand (Cisna) had a high frequency (43.3%) of the Nordic haplotype “c” suggesting that this provenance is derived from the Baltic post-glacial refugium of P. abies in Europe.
Źródło:
Leśne Prace Badawcze; 2017, 78, 1
1732-9442
2082-8926
Pojawia się w:
Leśne Prace Badawcze
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zróżnicowanie fenotypowe i zmienność tła genetycznego polskich proweniencji sosny zwyczajnej
Phenotypic differentiation and genetic background variation of Polish provenances of Pinus sylvestris L.
Autorzy:
Hebda, A.
Skrzyszewski, J.
Wachowiak, W.
Powiązania:
https://bibliotekanauki.pl/articles/987201.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
hodowla lasu
drzewa lesne
sosna zwyczajna
Pinus sylvestris
proweniencje krajowe
zmiennosc fenotypowa
zmiennosc genetyczna
markery mikrosatelitarne
DNA mikrosatelitarny
polimorfizm loci
zmiennosc adaptacyjna
scots pine
phenotypic differentiation
neutral variation
microsatellite markers
environment correlation
local adaptation
Opis:
We looked at the patterns of differentiation at a set of phenotypic traits of Scots pine populations derived from different environmental conditions of the species distribution range in Poland. The growth traits were tested over 47 years at the provenance trial experiment in the Carpathians. The phenotypic differentiation of the populations was compared to diversity estimates at a set of ten nuclear microsatellite markers. Scots pine populations were highly differentiated concerning diameter at breast high, stand volume, stem straightness and crown width according to the prove− nance and climatic zones. At the same time a low genetic diversity for microsatellite markers and no evidence of population structure was found among proveniences. Local populations from the Carpathian Mountains had lower genetic variation and weaker growth compared to lowland provenances. Among the tested populations Scots pine from lowlands were characterized by the great growth potential and good adaptation to the severe climate of the Carpathians. Populations that showed high quantitative traits differentiation were genetically homogenous at the neutral loci. The results indicate that there are some regions in the genome under selection that drive species adaptive variation.
Źródło:
Sylwan; 2017, 161, 04; 277-286
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sekwencje mikrosatelitarne i ich wykorzystanie w diagnostyce medycznej
Microsatellites and their application in diagnostics
Autorzy:
Korytko, Magdalena
Łaczmańska, Izabela
Powiązania:
https://bibliotekanauki.pl/articles/1034710.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Przyrodników im. Kopernika
Tematy:
diagnostyka genetyczna
DNA międzygenowy
sekwencje mikrosatelitarne
STR
QF-PCR
Opis:
Sekwencje mikrosatelitarne są to proste, tandemowe powtórzenia, zbudowane z jednego do sześciu nukleotydów, przy czym najczęściej identyfikowane mikrosatelity to sekwencje o dwunukleotydowym motywie powtórzeń (CA)n. Mikrosatelity występują w sekwencjach kodujących i w niekodujących fragmentach genów, jak i w obszarach pozagenowych i dziedziczone są zgodnie z prawami Mendla. W genomie człowieka sekwencje te pojawiają się średnio co sześć tysięcy par zasad. Ich duża zmienność polega na różnej liczbie powtórzeń motywu podstawowego w określonym miejscu (locus). Ze względu na wysoki stopień polimorfizmu, równomierne i częste rozmieszczenie w genomie są one doskonałymi markerami genetycznymi. Wykorzystywane są w konstruowaniu map genetycznych, kryminalistyce oraz analizie pokrewieństwa między osobnikami. W diagnostyce genetycznej polimorfizm sekwencji mikrosatelitarnych jest wykorzystywany głównie w tzw. diagnostyce pośredniej, w której analizuje się markery sprzężone z genem odpowiedzialnym za określoną jednostkę chorobową. Mikrosatelitarny DNA znalazł również zastosowanie w cytogenetyce molekularnej umożliwiając szybką diagnostykę najczęstszych aneuploidii w badaniach prenatalnych.
Microsatellite sequences or Short Tandem Repeats (STR) are simple, tandem (consecutive) repeats consisting of two to six nucleotides. Most often identified microsatellites are sequences with two-nucleotide repetition motif (CAn). Microsatellites occur in coding sequences, within non-coding gene fragments, as well as in outside gene areas, and are inherited according to Mendel's laws. In human genome these sequences occur at a frequency of one per six thousand base pairs. Their vast diversity consists of a various number of base motif repetitions in a given locus. Because of their high degree of polymorphism, regular and frequent occurrence in the genome, they make excellent genetic markers. They are used in the construction of genetic maps, forensics and in the analysis of relations between individuals. In molecular diagnostics the STR polymorphism is applied mainly in the so-called indirect diagnostics where STR markers connected with the gene responsible for a given disease are analyzed. Microsatellite DNA is also used in molecular cytogenetics making a rapid diagnosis of the most common aneuploidies in prenatal testing possible.
Źródło:
Kosmos; 2016, 65, 1; 11-16
0023-4249
Pojawia się w:
Kosmos
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New multiplex PCR assays for estimating genetic diversity in rainbow trout (Oncorhynchus mykiss) by polymorphism of microsatellite DNA
Autorzy:
Kaczmarczyk, D.
Kaczor, A.
Powiązania:
https://bibliotekanauki.pl/articles/363262.pdf
Data publikacji:
2013
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
microsatellite DNA
multiplex PCR
population genetics
rainbow trout
DNA mikrosatelitarne
genetyka populacyjna
pstrąg tęczowy
Opis:
Multiplex PCR is a useful technique for estimating genetic diversity. This paper presents 3 new sets of primer pairs for effectively amplifying 10 microsatellite DNA loci from rainbow trout (Oncorhynchus mykiss). Unlike other sets of primer pairs that have been developed for amplifying rainbow trout microsatellite loci, ours do not require the hot-start PCR technique. In the paper, we describe the steps taken to choose the loci for each multiplex assay and to verify the genotyping results. We provide the compositions of the PCR mixture and the characteristics of the PCR thermal profile recommended for amplification.
Źródło:
Environmental Biotechnology; 2013, 9, 1; 19-24
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Tracing the genetic origin of brown trout (Salmo trutta) re-colonizing the Ecker reservoir in the Hartz National Park, Germany
Autorzy:
Kohlmann, K.
Wüstemann, O.
Powiązania:
https://bibliotekanauki.pl/articles/363212.pdf
Data publikacji:
2012
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
pstrąg potokowy
Salmo trutta
mikrosatelitarne DNA
DNA
PCR
brown trout
microsatellite
Opis:
The Ecker reservoir and its main tributary had been free of brown trout (Salmo trutta) for several decades due to cumulative effects of natural and anthropogenic acidification. However, after the decline of emissions in the 1990s and the resulting rise of water pH to suitable for brown trout values, the species began to recolonize its original habitats. In the main tributary first brown trout individuals were caught in 2008 and in the reservoir in later years as well. Stocking could be excluded in both areas. Therefore, the present study was aimed to trace the genetic origin of these brown trout by genotyping eight microsatellite loci in samples collected in the reservoir, its main tributary, potential refugia and - for comparison - from two areas downstream of the dam being physically isolated for about 70 years. Genetic variability within populations (average number of alleles per locus), genetic differentiation between populations (FST values and genetic distances), occurrence of certain alleles and results of assignment tests indicated that the Ecker reservoir was re-colonized from two sources: the Große Peseke, a small direct inflow into the reservoir, and the Fuhler Lohnsbach, a parallel flowing brook connected to the reservoir by a pipe. Genetic data also supported recolonization of the main tributary from the reservoir but not in the opposite direction. Moreover, bottleneck effects were evident in brown trout populations upstream of the dam compared to the two populations downstream of the dam.
Źródło:
Environmental Biotechnology; 2012, 8, 2; 39-44
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Genetic identification of black caviar based on microsatellite DNA analysis
Autorzy:
Fopp-Bayat, D.
Powiązania:
https://bibliotekanauki.pl/articles/363094.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
identyfikacja genetyczna
czarny kawior
DNA mikrosatelitarne
genetic identification
black caviar
microsatellite DNA
Opis:
Sturgeons (Acipenseridae) are producers of black caviar, which is sold world-wide. Black caviar differs widely in quality, availability, price and taste and for these reasons it is often the subject of commercial fraud. Identification of sturgeon species is frequently based on molecular methods such as analysis of nuclear DNA and mitochondrial DNA. In this study two fragments of microsatellite DNA: Afu-39 and Afu-68 were analyzed. In caviar samples, four alleles were observed in locus Afu-39, and six alleles in locus Afu-68. This paper describes the use of microsatellite DNA markers for species identification of black caviar.
Źródło:
Environmental Biotechnology; 2007, 3, 2; 57-60
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polymorphism of microsatellite loci - a tool in studying biodiversity of paddlefish aquaculture broodstock
Autorzy:
Kaczmarczyk, D.
Kohlmann, K.
Kersten, P.
Luczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/363088.pdf
Data publikacji:
2007
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
izolacja DNA
DNA mikrosatelitarne
amplifikacja PCR
DNA extraction
microsatellite DNA
PCR amplification
Opis:
American paddlefish (Polyodon spathula) is a new species in Polish aquaculture, its broodstocks are few and small, and it is possible that all mature fish originated from only a few spawners. Studies on polymorphism of highly variable microsatellite DNA allow revealing genetic characteristics of individual spawners as well as estimation of genetic variation within and divergence between broodstocks. This paper describes optimised protocols for isolation of DNA from fin tissues, amplification of nine microsatellite loci using PCR technique, and for fish genotyping using automatic capillary DNA sequencer. Our technique was tested towards the fin samples taken from all paddlefish reared in Poland and approaching their sexual maturity; the study included also samples taken from 47 fish of the Ukrainian breeding center (Gorny Tykich).
Źródło:
Environmental Biotechnology; 2007, 3, 2; 44-48
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microsatellite DNA polymorphism in sturgeon species and their hybrids reared in Polish aquaculture farms
Autorzy:
Fopp-Bayat, D.
Luczynski, M.
Powiązania:
https://bibliotekanauki.pl/articles/363194.pdf
Data publikacji:
2006
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:
jesiotr
identyfikacja gatunkowa
DNA mikrosatelitarne
hybrydy
polimorfizm
hybrids
microsatellite DNA
polymorphism
PCR
species identification
sturgeons
Opis:
Highly variable microsatellite DNA loci show high levels of variation per locus and provide molecular markers for species and for populations of small effective size. In this study we applied microsatellite markers to identify specimens belonging to several sturgeon species and their interspecific hybrids. Nine microsatellite loci (Afu-19, Afu-22, Afu-34, Afu-39, Afu-54, Afu-57, Afu-58, Afu-68, Afu-69) were analysed for five species (beluga, Siberian sturgeon, Russian sturgeon, sterlet and paddlefish) and for their three interspecific hybrids: Siberian sturgeon x Russian sturgeon, beluga x sterlet (called bester) and for beluga x bester. Certain alleles at five loci: Afu-22, Afu-39, Afu-54, Afu-57 and Afu-68 were diagnostic for the examined sturgeon species and their interspecific hybrids.
Źródło:
Environmental Biotechnology; 2006, 2, 1; 11-19
1734-4964
Pojawia się w:
Environmental Biotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
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