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    Tytuł:
    Polymorphism of microsatellite loci - a tool in studying biodiversity of paddlefish aquaculture broodstock
    Autorzy:
    Kaczmarczyk, D.
    Kohlmann, K.
    Kersten, P.
    Luczynski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363088.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    izolacja DNA
    DNA mikrosatelitarne
    amplifikacja PCR
    DNA extraction
    microsatellite DNA
    PCR amplification
    Opis:
    American paddlefish (Polyodon spathula) is a new species in Polish aquaculture, its broodstocks are few and small, and it is possible that all mature fish originated from only a few spawners. Studies on polymorphism of highly variable microsatellite DNA allow revealing genetic characteristics of individual spawners as well as estimation of genetic variation within and divergence between broodstocks. This paper describes optimised protocols for isolation of DNA from fin tissues, amplification of nine microsatellite loci using PCR technique, and for fish genotyping using automatic capillary DNA sequencer. Our technique was tested towards the fin samples taken from all paddlefish reared in Poland and approaching their sexual maturity; the study included also samples taken from 47 fish of the Ukrainian breeding center (Gorny Tykich).
    Źródło:
    Environmental Biotechnology; 2007, 3, 2; 44-48
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic identification of black caviar based on microsatellite DNA analysis
    Autorzy:
    Fopp-Bayat, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/363094.pdf
    Data publikacji:
    2007
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    identyfikacja genetyczna
    czarny kawior
    DNA mikrosatelitarne
    genetic identification
    black caviar
    microsatellite DNA
    Opis:
    Sturgeons (Acipenseridae) are producers of black caviar, which is sold world-wide. Black caviar differs widely in quality, availability, price and taste and for these reasons it is often the subject of commercial fraud. Identification of sturgeon species is frequently based on molecular methods such as analysis of nuclear DNA and mitochondrial DNA. In this study two fragments of microsatellite DNA: Afu-39 and Afu-68 were analyzed. In caviar samples, four alleles were observed in locus Afu-39, and six alleles in locus Afu-68. This paper describes the use of microsatellite DNA markers for species identification of black caviar.
    Źródło:
    Environmental Biotechnology; 2007, 3, 2; 57-60
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    New multiplex PCR assays for estimating genetic diversity in rainbow trout (Oncorhynchus mykiss) by polymorphism of microsatellite DNA
    Autorzy:
    Kaczmarczyk, D.
    Kaczor, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/363262.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    microsatellite DNA
    multiplex PCR
    population genetics
    rainbow trout
    DNA mikrosatelitarne
    genetyka populacyjna
    pstrąg tęczowy
    Opis:
    Multiplex PCR is a useful technique for estimating genetic diversity. This paper presents 3 new sets of primer pairs for effectively amplifying 10 microsatellite DNA loci from rainbow trout (Oncorhynchus mykiss). Unlike other sets of primer pairs that have been developed for amplifying rainbow trout microsatellite loci, ours do not require the hot-start PCR technique. In the paper, we describe the steps taken to choose the loci for each multiplex assay and to verify the genotyping results. We provide the compositions of the PCR mixture and the characteristics of the PCR thermal profile recommended for amplification.
    Źródło:
    Environmental Biotechnology; 2013, 9, 1; 19-24
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microsatellite DNA polymorphism in sturgeon species and their hybrids reared in Polish aquaculture farms
    Autorzy:
    Fopp-Bayat, D.
    Luczynski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/363194.pdf
    Data publikacji:
    2006
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    jesiotr
    identyfikacja gatunkowa
    DNA mikrosatelitarne
    hybrydy
    polimorfizm
    hybrids
    microsatellite DNA
    polymorphism
    PCR
    species identification
    sturgeons
    Opis:
    Highly variable microsatellite DNA loci show high levels of variation per locus and provide molecular markers for species and for populations of small effective size. In this study we applied microsatellite markers to identify specimens belonging to several sturgeon species and their interspecific hybrids. Nine microsatellite loci (Afu-19, Afu-22, Afu-34, Afu-39, Afu-54, Afu-57, Afu-58, Afu-68, Afu-69) were analysed for five species (beluga, Siberian sturgeon, Russian sturgeon, sterlet and paddlefish) and for their three interspecific hybrids: Siberian sturgeon x Russian sturgeon, beluga x sterlet (called bester) and for beluga x bester. Certain alleles at five loci: Afu-22, Afu-39, Afu-54, Afu-57 and Afu-68 were diagnostic for the examined sturgeon species and their interspecific hybrids.
    Źródło:
    Environmental Biotechnology; 2006, 2, 1; 11-19
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Tracing the genetic origin of brown trout (Salmo trutta) re-colonizing the Ecker reservoir in the Hartz National Park, Germany
    Autorzy:
    Kohlmann, K.
    Wüstemann, O.
    Powiązania:
    https://bibliotekanauki.pl/articles/363212.pdf
    Data publikacji:
    2012
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    pstrąg potokowy
    Salmo trutta
    mikrosatelitarne DNA
    DNA
    PCR
    brown trout
    microsatellite
    Opis:
    The Ecker reservoir and its main tributary had been free of brown trout (Salmo trutta) for several decades due to cumulative effects of natural and anthropogenic acidification. However, after the decline of emissions in the 1990s and the resulting rise of water pH to suitable for brown trout values, the species began to recolonize its original habitats. In the main tributary first brown trout individuals were caught in 2008 and in the reservoir in later years as well. Stocking could be excluded in both areas. Therefore, the present study was aimed to trace the genetic origin of these brown trout by genotyping eight microsatellite loci in samples collected in the reservoir, its main tributary, potential refugia and - for comparison - from two areas downstream of the dam being physically isolated for about 70 years. Genetic variability within populations (average number of alleles per locus), genetic differentiation between populations (FST values and genetic distances), occurrence of certain alleles and results of assignment tests indicated that the Ecker reservoir was re-colonized from two sources: the Große Peseke, a small direct inflow into the reservoir, and the Fuhler Lohnsbach, a parallel flowing brook connected to the reservoir by a pipe. Genetic data also supported recolonization of the main tributary from the reservoir but not in the opposite direction. Moreover, bottleneck effects were evident in brown trout populations upstream of the dam compared to the two populations downstream of the dam.
    Źródło:
    Environmental Biotechnology; 2012, 8, 2; 39-44
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sekwencje mikrosatelitarne i ich wykorzystanie w diagnostyce medycznej
    Microsatellites and their application in diagnostics
    Autorzy:
    Korytko, Magdalena
    Łaczmańska, Izabela
    Powiązania:
    https://bibliotekanauki.pl/articles/1034710.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Przyrodników im. Kopernika
    Tematy:
    diagnostyka genetyczna
    DNA międzygenowy
    sekwencje mikrosatelitarne
    STR
    QF-PCR
    Opis:
    Sekwencje mikrosatelitarne są to proste, tandemowe powtórzenia, zbudowane z jednego do sześciu nukleotydów, przy czym najczęściej identyfikowane mikrosatelity to sekwencje o dwunukleotydowym motywie powtórzeń (CA)n. Mikrosatelity występują w sekwencjach kodujących i w niekodujących fragmentach genów, jak i w obszarach pozagenowych i dziedziczone są zgodnie z prawami Mendla. W genomie człowieka sekwencje te pojawiają się średnio co sześć tysięcy par zasad. Ich duża zmienność polega na różnej liczbie powtórzeń motywu podstawowego w określonym miejscu (locus). Ze względu na wysoki stopień polimorfizmu, równomierne i częste rozmieszczenie w genomie są one doskonałymi markerami genetycznymi. Wykorzystywane są w konstruowaniu map genetycznych, kryminalistyce oraz analizie pokrewieństwa między osobnikami. W diagnostyce genetycznej polimorfizm sekwencji mikrosatelitarnych jest wykorzystywany głównie w tzw. diagnostyce pośredniej, w której analizuje się markery sprzężone z genem odpowiedzialnym za określoną jednostkę chorobową. Mikrosatelitarny DNA znalazł również zastosowanie w cytogenetyce molekularnej umożliwiając szybką diagnostykę najczęstszych aneuploidii w badaniach prenatalnych.
    Microsatellite sequences or Short Tandem Repeats (STR) are simple, tandem (consecutive) repeats consisting of two to six nucleotides. Most often identified microsatellites are sequences with two-nucleotide repetition motif (CAn). Microsatellites occur in coding sequences, within non-coding gene fragments, as well as in outside gene areas, and are inherited according to Mendel's laws. In human genome these sequences occur at a frequency of one per six thousand base pairs. Their vast diversity consists of a various number of base motif repetitions in a given locus. Because of their high degree of polymorphism, regular and frequent occurrence in the genome, they make excellent genetic markers. They are used in the construction of genetic maps, forensics and in the analysis of relations between individuals. In molecular diagnostics the STR polymorphism is applied mainly in the so-called indirect diagnostics where STR markers connected with the gene responsible for a given disease are analyzed. Microsatellite DNA is also used in molecular cytogenetics making a rapid diagnosis of the most common aneuploidies in prenatal testing possible.
    Źródło:
    Kosmos; 2016, 65, 1; 11-16
    0023-4249
    Pojawia się w:
    Kosmos
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Określenie pochodzenia wyłączonych drzewostanów nasiennych sosny rychtalskiej (Pinus sylvestris L.) z wykorzystaniem markerów mikrosatelitarnych
    Determination of the origin of the rychtal Scots pine (Pinus sylvestris L.) seed tree stands using microsatellite markers
    Autorzy:
    Wójkiewicz, B.
    Żukowska, W.B.
    Urbaniak, L.
    Kowalczyk, J.
    Litkowiec, M.
    Lewandowski, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/985719.pdf
    Data publikacji:
    2019
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    hodowla lasu
    sosna zwyczajna
    Pinus sylvestris
    sosna rychtalska
    drzewostany nasienne
    pochodzenie roslin
    zmiennosc genetyczna
    analiza DNA
    markery mikrosatelitarne
    scots pine
    genetic variation
    gene pool
    genetic structure
    ssr markers
    Opis:
    The rychtal pine is one of the most valuable ecotypes of Scots pine (Pinus sylvestris L.) approved for the breeding purposes in Poland. However, it occupies stands typical for oaks and beeches as shown by the compatibility analysis of species composition in relation to the habitat type in which they occur. Such result raises some doubts in terms of the naturalness of the rychtal pine and calls its history and origin into question. In the present study, we used the set of nuclear microsatellite markers to characterize and compare the gene pool composition of the selected seed tree stands of the rychtal pine with 200−year−old pine trees which grow at the Syców Forest District (SW Poland). We aimed to know to what extent the set of alleles specified for the group of the oldest trees from natural habitats is represented in the younger forest tree stands of the rychtal pine. The analysis of molecular variance (AMOVA) and clustering analysis showed that the gene pool of the studied pine populations was homogenous (FST=0,02%, K=1). The parameters of genetic variation were similar for all populations except for the mean number of alleles. On average, 25 new alleles were found in two rychtal pine seed tree stands as compared to the set of alleles found in the group of old pine trees. However, all alleles defined for old pines were also present in the gene pool of younger rychtal pine forest stands. The differences in the gene pool richness result most likely from quite high differences in the number of individuals analyzed from each population. In conclusion, our results indicate the common origin of the studied Scots pine populations.
    Źródło:
    Sylwan; 2019, 163, 08; 637-644
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Stan zdrowotny a zróżnicowanie genetyczne wybranych drzewostanów świerkowych na terenie RDLP w Krośnie
    Genetic variability and health of Norway spruce stands in the Regional Directorate of the State Forests in Krosno
    Autorzy:
    Gutkowska, J.
    Borys, M.
    Tereba, A.
    Tkaczyk, M.
    Oszako, T.
    Nowakowska, J.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/1293265.pdf
    Data publikacji:
    2017
    Wydawca:
    Instytut Badawczy Leśnictwa
    Tematy:
    lesnictwo
    RDLP Krosno
    drzewostany swierkowe
    stan zdrowotny lasu
    drzewa lesne
    swierk pospolity
    Picea abies
    zroznicowanie genetyczne
    markery molekularne
    markery mikrosatelitarne
    DNA mitochondrialny
    Norway spruce
    health state
    SSR markers
    mtDNA
    genetic diversity
    Opis:
    The study was conducted in 2015 in six spruce stands situated in different forest districts administratively belonging to the Regional Directorate of Forests State in Krosno. Each spruce population was represented by 30 trees and assessed in terms of their current health status. Genetic analyses were performed based on shoot samples from each tree using nine nuclear DNA markers and one mitochondrial DNA marker (nad1). The health status of the trees was described according to the classification developed by Szczepkowski and Tarasiuk (2005) and the correlation between health classes and the level of genetic variability was computed with STATISTICA (α = 0.05). Nuclear DNA analyses revealed a low level of genetic variability among spruce populations (only 3% of the total genetic variation (FST = 0.028) and a high variability within populations (97%). The total heterozygosity in all stands (HT) was calculated as 0.646. Based on UPGMA analysis, the most genetically similar populations are spruce stands in the Bieszczady National Park and the Ustrzyki Dolne Forest District, which have the smallest genetic divergence of all populations (DN = 0.0165). Our analysis of the mitochondrial gene nad1 revealed the presence of six different haplotypes “a”, “a1”, “b”, “c”, “d” and “d1”. Comprising 56% of all haplotypes, “a” was the most common showing a predominant impact on spruce migration from the Carpathian area. The analysis based on mitochondrial markers (by Nei) revealed a heterozygosity of 0.525. Based on the observations of disease symptoms, 29% of the trees belong to health class 1,30% to class 2,28% to class 3 and class 4 contains 13% of trees. The comparison between health status and the level of genetic variation in the analyzed stands showed a positive correlation. Spruce stands with better health were also characterized by a greater degree of genetic variability. Since most of the investigated spruce populations shared the mitochondrial haplotype “a”, we have ascertained their Hercynian- Carpathian origin. Only one stand (Cisna) had a high frequency (43.3%) of the Nordic haplotype “c” suggesting that this provenance is derived from the Baltic post-glacial refugium of P. abies in Europe.
    Źródło:
    Leśne Prace Badawcze; 2017, 78, 1
    1732-9442
    2082-8926
    Pojawia się w:
    Leśne Prace Badawcze
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Porównanie zmienności genetycznej pokolenia matecznego i sztucznie wyhodowanego potomstwa sosny zwyczajnej na podstawie analiz DNA
    Comparison of the genetic variability of Scots pine trees and their progeny from nursery production based on DNA analyses
    Autorzy:
    Konecka, A.
    Tereba, A.
    Bieniek, J.
    Nowakowska, J.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/985843.pdf
    Data publikacji:
    2018
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    hodowla lasu
    sosna zwyczajna
    Pinus sylvestris
    zmiennosc genetyczna
    analiza DNA
    markery mikrosatelitarne
    drzewa mateczne
    drzewa potomne
    sadzonki z zakrytym systemem korzeniowym
    genetic diversity
    ssr markers
    forest nursery production
    pinus sylvestris l.
    Opis:
    The production of forest tree species in forest nurseries is performed via two main breeding systems: i) the traditional (conventional) way with the seedlings grown in soil, and ii) plants cultivated in the containers. The aim of the study was to assess the level of genetic variability in the populations of the mother stands and the progeny populations of Scots pine cultured with traditional way (in soil) and in containers in two nurseries in Olsztynek (N Poland) and Oleszyce (S Poland) forest districts. Four polymorphic microsatellite markers (SPAG 7.14, SPAC 11.6, SPAC 12.5 and SsrPt_ctg4363) were used to evaluate the genetic variability of the studied populations. The basic hypothesis assumed that higher gene pool characterizes the seedlings grown in the containers comparing to the seedlings grown in the ground. The results confirmed that. Seedlings from containerized breeding had larger gene pool and were more diverse than plants with conventional breeding, both in Olsztynek and Oleszyce. Our study revealed a significant human impact on shaping the pool of forest genetic resources of Polish forests at the early stage of nursery production and showed the need for a broader study on further stages of cultivation of forests.
    Źródło:
    Sylwan; 2018, 162, 01; 32-40
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zróżnicowanie fenotypowe i zmienność tła genetycznego polskich proweniencji sosny zwyczajnej
    Phenotypic differentiation and genetic background variation of Polish provenances of Pinus sylvestris L.
    Autorzy:
    Hebda, A.
    Skrzyszewski, J.
    Wachowiak, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/987201.pdf
    Data publikacji:
    2017
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    hodowla lasu
    drzewa lesne
    sosna zwyczajna
    Pinus sylvestris
    proweniencje krajowe
    zmiennosc fenotypowa
    zmiennosc genetyczna
    markery mikrosatelitarne
    DNA mikrosatelitarny
    polimorfizm loci
    zmiennosc adaptacyjna
    scots pine
    phenotypic differentiation
    neutral variation
    microsatellite markers
    environment correlation
    local adaptation
    Opis:
    We looked at the patterns of differentiation at a set of phenotypic traits of Scots pine populations derived from different environmental conditions of the species distribution range in Poland. The growth traits were tested over 47 years at the provenance trial experiment in the Carpathians. The phenotypic differentiation of the populations was compared to diversity estimates at a set of ten nuclear microsatellite markers. Scots pine populations were highly differentiated concerning diameter at breast high, stand volume, stem straightness and crown width according to the prove− nance and climatic zones. At the same time a low genetic diversity for microsatellite markers and no evidence of population structure was found among proveniences. Local populations from the Carpathian Mountains had lower genetic variation and weaker growth compared to lowland provenances. Among the tested populations Scots pine from lowlands were characterized by the great growth potential and good adaptation to the severe climate of the Carpathians. Populations that showed high quantitative traits differentiation were genetically homogenous at the neutral loci. The results indicate that there are some regions in the genome under selection that drive species adaptive variation.
    Źródło:
    Sylwan; 2017, 161, 04; 277-286
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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