- Tytuł:
- The evaluation of topography Borrelia burgdorferi by atomic force microscope (AFM)
- Autorzy:
-
Tokarska-Rodak, M.
Kozioł-Montewka, M. - Powiązania:
- https://bibliotekanauki.pl/articles/2052368.pdf
- Data publikacji:
- 2015
- Wydawca:
- Akademia Bialska Nauk Stosowanych im. Jana Pawła II w Białej Podlaskiej
- Tematy:
-
Borrelia burgdorferi s.s B31
AFM
spirochetes’ cells - Opis:
- The aim of the present work was to evaluate the topography of spirochetes’ cells Borrelia burgdorferi s.s. B31 in atomic force microscope (AFM). Results: The length of spirochetes B. burgdorferi has ranged between 15.38-22.68μm. The cells of spirochetes do not constitute structures of a fixed diameter and height. Thus, in order to identify real parameters of cells, the horizontal distance and vertical distance have been used in the measurements. The average value of a spirochetes’ diameter has been estimated by taking series of measures and it is 0.40 μm. The average value of a spirochetes’ height has been estimated by taking series of measures and it is 70.14 nm. The analysis of a relation between measured parameters of spirochetes: diameter and height revealed that along with the growth of diameter of a bacteria cell, its height also grows. The average value of a fibers’ diameter has been estimated by taking series of measurements and it is 0.09 μm and the average height of fibers was 7.91 nm. Conclusions: The atomic force microscope (AFM) is a modern tool with a broad spectrum of observatory and measure abilities and is a technique which has been used in biology and microbiology to investigate the topography of surface and in the evaluation properties of cells.
- Źródło:
-
Health Problems of Civilization; 2015, 09, 3; 12-15
2353-6942
2354-0265 - Pojawia się w:
- Health Problems of Civilization
- Dostawca treści:
- Biblioteka Nauki
Artykuł