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    Wyświetlanie 1-44 z 44
    Tytuł:
    Influence of magnetic fields on the activity of enzymes: alpha- and beta-amylase and glutathione S-transferase [GST] in wheat plants
    Autorzy:
    Rochalska, M.
    Grabowska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/25053.pdf
    Data publikacji:
    2007
    Wydawca:
    Polska Akademia Nauk. Instytut Agrofizyki PAN
    Tematy:
    wheat plant
    magnetic field
    glutathione S-transferase
    beta-amylase
    enzyme
    alpha-amylase
    magnetic biostimulation
    germination
    Źródło:
    International Agrophysics; 2007, 21, 2
    0236-8722
    Pojawia się w:
    International Agrophysics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Impact of feed supplement with alfa-amylase and beta-glucanase on ingestive-related biomarkers registered with real-time sensors
    Autorzy:
    Antanaitis, R.
    Anskienė, L.
    Televičius, M.
    Malašauskienė, D.
    Šertvytytė, G.
    Stoškus, R.
    Meškinytė, E.
    Rous, J.
    Helm, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/16626661.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    alfa-amylase
    beta-glucanase
    reticulorumen
    rumination
    Opis:
    The aim of this study was to investigate the impact of feed supplements with alfa-amylase and beta-glucanase (Optipartum C+ 200) on ingestive-related behaviour biomarkers registered with real-time sensors: rumination behaviours and reticulorumen parameters (pH and temperature). Cows (n=20) in the treatment group (TG) were fed with Optipartum C+ 200 (Enzymes feed supplement: Alfa-Amylase 57 Units; Beta-Glucanase 107 Units) from 21 days before calving until 30 days after calving with a feeding rate of 200 g/cow/day. Cows (n=22) in the control group (CG) were fed a feed ration without feed supplement. Measurements started from 6 days before calving and continued until 21 days after calving. The following indicators were registered: with the RumiWatch System: Rumination time; Eating time; Drinking time; Rumination chews; Eating chews; Drinking gulps; Bolus; Chews per minute; Chews per bolus. With the SmaXtec system: the temperature, pH of the contents of the cows’ reticulorumens, and cows’ walking activity. According to our results, feed supplementation with alfa-amylase and beta-glucanase (Optipartum C+ 200) in the TG group resulted in increases in the following parameters: 9% rumination time and eating time, 19% drinking time, 11% rumination chews, 16% eating chews, 13% number of boluses per rumination, 5% chews per minute and 16% chews per bolus. The rumination time showed a strong, positive relation with rumination chews and bolus indicators in both groups (TG and CG) (p<0.001); while the rumination time in both groups of cows showed an opposite direction and was negatively related to eating time and eating chews (p<0.05). We found a 1.28 % lower reticulorumen pH and a 0.64 % lower reticulorumen temperature in cows fed with the supplement compared with cows in the control group. Cows in TG were 8.80% more active than those in the CG group. For improvement of ingestive-related behaviour we suggest adding a feed supplement with alfa-amylase and beta-glucanase (Optipartum C+ 200).
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 4; 535-546
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of salivary alpha amylase activity in smokers with periodontitis, Khartoum state, 2023
    Autorzy:
    Abd Alwhab Awad Allah, Fatima
    Elrheima Ahmed, Hafsa Ahmed
    Powiązania:
    https://bibliotekanauki.pl/articles/29519597.pdf
    Data publikacji:
    2023-12-31
    Wydawca:
    Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
    Tematy:
    alpha-amylase
    periodontitis
    saliva
    smokers
    Opis:
    Introduction and aim. Smoking widely affect oral health, including its role in the development of periodontitis. Saliva contains an antioxidant system and various enzymes. The study was designed to evaluate the activity of salivary alpha amylase among individuals who smoke and have periodontitis. Material and methods. A total of 100 participants were included, with 50 cases (cigarette smokers with periodontitis) and the remaining 50 nonsmokers with healthy periodontium as the control group. Saliva samples were collected to measure salivary alpha amylase activity. Results. Smokers with periodontitis had significantly higher levels of salivary alpha amylase compared to the control group(177.96±14.5 vs 94.04±19.6 IU/mL, p<0.001). Additionally, there was a weak negative correlation between the level of alpha amylase and the age of the patients (p=0.01, r=0.376). However, no correlation was found between the level of alpha amylase and the duration of smoking (p=0.584, r=0.079). Conclusion. There is a significant increase in salivary alpha amylase levels among smokers with periodontitis. No correlation was found between age and salivary alpha amylase levels. However, a weak positive correlation was observed between the duration of smoking and salivary alpha amylase activity.
    Źródło:
    European Journal of Clinical and Experimental Medicine; 2023, 4; 692-695
    2544-2406
    2544-1361
    Pojawia się w:
    European Journal of Clinical and Experimental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Physical and chemical properties of sweet juice produced from hydrolysed acha (Digitaria exilis Stapf) starch using crude amylase from germinated maize
    Autorzy:
    Abdulquadri, O. Alaka
    Rahman, Akinoso
    Powiązania:
    https://bibliotekanauki.pl/articles/1179002.pdf
    Data publikacji:
    2017
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Maize amylase
    acha
    germinative capacity
    juice
    starch
    Opis:
    This study investigated the possibility of producing juice by hydrolyzing acha starch using crude amylase from germinated maize. Planted maize was monitored for seven days to determine germinative capacity and amylase activity of the seed. Extracted acha starch was hydrolysed using wet homogenised maize seedlings in an incubator at 62-68 °C for 6-8 hours. Colour, pH, titratable acidity (TTA), °Brix and sensory attribute of the juice were determined using standard method. A commercial malt beverage was used as reference. The maize sample had a mean capacity of 95% while its maximum amylase activity occurred at day 4. The pH, TTA, and °Brix of the juice were obtained as 4.5, 0.85 and 10 respectively. Colour values for L*, a*, and b* were 37.64, 7.6, and 15.3 respectively. Sensory analysis showed no significant difference between the juice and reference sample in terms of taste and colour. However, significant differences were observed in aroma and overall acceptability. Hence, malt beverages can be produced using this method but requires certain additives to impart prevalent aroma associated with malt beverages.
    Źródło:
    World Scientific News; 2017, 87; 125-135
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of steeping degree, malting temperature and addition of gibberellic acid on the accumulation of alpha-amylase in malt
    Wpływ stopnia namoczenia, temperatury słodowania i dodatku kwasu giberelinowego na nagromadzenie alfa-amylazy w słodzie
    Autorzy:
    Surmiński, J.
    Masior, S.
    Kuchciak, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/1398778.pdf
    Data publikacji:
    1986
    Wydawca:
    Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
    Tematy:
    malting harley
    a-amylase
    giberellic acid
    Opis:
    Malting on a microtechnological scale was performed with the use of brewer's barley of Trumpf and Polon varieties and with the feed variety Diva, all from the 1982 crop. The highest α-amylase content was found in the Polon variety; this content in the Trumpf variety was lower by about 7%, while in the feed variety Diva it was lower by about 20%. The following conditions were found to favour the accumulation of α-amylase in malt containing no addition of gibberellic acid (g.a.): malted grain humidity - 46%, malting temperature - 18°C, time of malting - 7 days. When gibberellic acid was added in doses of 0.2-0.3 mg/kg barley and when pH of the water with g.a. ranged from 6.5 to 7.5, the mean increase of α-amylase content in the studied malt varieties was 54% as compared to control samples without g.a. In the case of the variety Diva this increase was 57%. The conditions of malting with an addition of g.a. that were worked out allow the production of brewer's malt from Diva barley.
    Do słodowania w skali mikrotechnicznej użyto krajowe odmiany browarne jęczmienia: Trumpf i Polon oraz odmianę paszową Diva ze zbioru 1982 r. Określano wpływ zmienności warunków słodowania: namoczenie jęczmienia do 43 i 46%, czas słodowania 7 i 9 dni, pH wody z dodatkiem kwasu giberelinowego (K.G.) od 5,5 do 8,5, dawki K.G. w zakresie 0,2, 0,3 i 0,5 mg/kg. W doświadczalnych jęczmieniach oznaczano: wilgotność, ekstraktywność, białko ogółem, skrobię, wagę 1000 ziaren, celność ziarna i energię kiełkowania. Analiza słodów obejmowała: wilgotność, ekstraktywność w mące i śrucie, białko ogółem, białko taninowe, barwę brzeczki, liczbę Kolbacha, garbniki ogółem, lepkość brzeczki, azot α-aminokwasowy, zawartość α-amylazy (metodą maltozową Briggsa), siłę diastatyczną według Windisch-Kolbacha, zawartość endo-β-glukanazy i zawartość endopeptydaz. Najwyższe zawartości α-amylazy (tab. 2) dawała odmiana Polon (570 j. FS), niższe Trumpf (o ok. 7%), najniższe odmiana paszowa Diva (o ok. 20%). Za korzystne warunki nagromadzania α-amylazy w słodzie bez dodatku kwasu giberelinowego (K.G.) uznano: namoczenie ziarna do wilgotności ok. 46%, temperaturę słodowania ok. 18°C i czas słodowania 7 dni (tab. 2 i 3). Przy ilościach dodawanego kwasu giberelinowego od 0,2 do 0,3 mg/kg jęczmienia i pH wody z K.G. od 6,5 do 7,5 średni przyrost zawartości α-amylazy dla badanych odmian wynosił 54% w porównaniu z próbami kontrolnymi bez K.G. (tab. 5). Dla odmiany Diva przyrost ten wynosił 57%. Opracowane warunki słodowania z dodatkiem K.G. stwarzają możliwości produkcji z jęczmienia odmiany Diva słodu piwowarskiego.
    Źródło:
    Acta Alimentaria Polonica; 1986, 12, 2; 91-101
    0137-1495
    Pojawia się w:
    Acta Alimentaria Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    DIPSACUS FULLONUM L. LEAVES AND ROOTS - IDENTIFICATION OF THE COMPONENTS OF THE ESSENTIAL OILS AND ALPHA-AMYLASE INHIBITORY ACTIVITIES OF METHANOLIC EXTRACTS
    Autorzy:
    Witkowska-Banaszczak, Ewa
    Powiązania:
    https://bibliotekanauki.pl/articles/895320.pdf
    Data publikacji:
    2018-08-31
    Wydawca:
    Polskie Towarzystwo Farmaceutyczne
    Tematy:
    essential oil composition
    methanolic extract
    alpha-amylase inhibition
    Opis:
    Abstract: Dipsacus fullonum L. syn. D. sylvestris is a biennial plant from the Dipsacaceae family. It grows in Europe, North Africa and Asia Minor. It has been used in folk medicine mainly to treat Lyme disease. This study was undertaken to identify and compare the components of the essential oils from dried and fresh roots, which are usually applied as medicinal agents, as well as those of the essential oils from dried and fresh leaves, used less frequently. The essential oils from the Dipsacus fullonum leaves and roots were analysed by the GC and GC-MS analysis. 18 components were identified in the essential oil from the fresh leaves, whereas 37 were recognised in the oil from the dried leaves; 10 components were identified in the essential oil from the fresh roots and 40 in that from the dried roots. Phytol was the main component in the essential oil from the leaves, whereas n-hexadecanoic acid was predominant in the roots. The α -amylase inhibition has been proven for methanolic extracts of D. fullonum leaves and roots. The strongest activity was demonstrated by the extract from fresh leaves of D. fullonum and showed an effect about one hundred times weaker than acarbose.
    Źródło:
    Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 4; 951-957
    0001-6837
    2353-5288
    Pojawia się w:
    Acta Poloniae Pharmaceutica - Drug Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Polycarbonate biodegradation by newly isolated Bacillus strains
    Autorzy:
    Arefian, Mojgan
    Tahmourespour, Arezoo
    Zia, Mohammadali
    Powiązania:
    https://bibliotekanauki.pl/articles/205230.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    biodegradation
    Bacillus cereus
    amylase
    lipase
    Polycarbonate
    Bacillus megaterium
    Opis:
    As polycarbonate is frequently used in many products, its accumulation in landfills is absolutely harmful to the environment. The aims of this study were the screening and isolation of polycarbonate-degrading bacteria (PDB) and the assessment of their ability in the degradation of polycarbonate (PC) polymers. Nine-month buried- -PC films were used for PDB isolation and identification. The biodegradation ability of the isolates was determined by growth curve, clear zone formation, lipase and amylase production, AFM and FTIR. Bacillus cereus and Bacillus megaterium were identified and considered as PDB. The degradation ability of B. megaterium was significantly higher than that of B. cereus. Both were lipase and amylase positive. AFM and FTIR results showed the initiation of bacterial attachment. The PC biodegradation ability of isolates can be very efficient. Finding such efficient isolates (which was less studied before) will promise a decrease in plastic contamination in the future.
    Źródło:
    Archives of Environmental Protection; 2020, 46, 1; 14-20
    2083-4772
    2083-4810
    Pojawia się w:
    Archives of Environmental Protection
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Activity of glycogen depolymerizing enzymes in extracts from brain tumor tissue (anaplastic astrocytoma and glioblastoma multiforme).
    Autorzy:
    Kotoński, Bogusław
    Wilczek, Joanna
    Madej, Janusz
    Zarzycki, Andrzej
    Hutny, Jan
    Powiązania:
    https://bibliotekanauki.pl/articles/1044051.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    glioblastoma
    α-amylase
    α-glucosidase
    phosphorylase
    astrocytoma
    Opis:
    An approximately threefold increase in glycogenolytic activity of the neutral α-1,4-glucosidase and a twofold increase in the same activity of the acid isoform have been found in extracts of anaplastic astrocytoma and glioblastoma multiforme tumors of brain tissue. "Maltase activity" of the respective enzymes increased by 60-80% in both kinds of tumor extracts. However a significant decrease in α-amylase and almost complete disappearance of phosphorylase activities have also been found in both kinds of tumors.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 4; 1085-1090
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of post-harvest maturation on the amylolytic activity of wheat grain and the properties of bread
    Autorzy:
    Szafrańska, Anna
    Stępniewska, Sylwia
    Powiązania:
    https://bibliotekanauki.pl/articles/2148191.pdf
    Data publikacji:
    2021-12-30
    Wydawca:
    Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy
    Tematy:
    alpha-amylase
    bread
    mixolab
    technological value
    wheat
    Opis:
    The aim of this study was to determine the quantitative and qualitative changes in the starch complex and the amylolytic activity of wheat grain during its post-harvest maturity. This in- formation is necessary for the proper determination of the level of quality requirements for wheat grain purchased immediately after harvest. The common wheat cultivars: Kandela, Bamberka and KWS Ozon were tested during this study. Because of the variability of weather conditions, three wheat cultivars were tested (two winter wheat cultivars and one spring wheat cultivar) from two consecu- tive crop years. Immediately after grain harvest and during the next 12 weeks in two-weeks intervals the falling number, amylo- graph properties, rheological properties of dough and laboratory baking test was performed. Tested wheat cultivars were characterized by low amylolytic activity. Changes of the baking value of tested wheat cultivars during their post-harvest maturation were dependent on wheat cultivar and harvest year. Wheat from the 2016 harvest was char- acterized by significantly lower amylolytic activity compared to grain from 2015 harvest. Amylolytic activity decreased during post-harvest maturation, i.e. the falling number increased as well as the dough torque in point C4 and C5 of mixolab curve. There was also an increase in the volume of bread compared to the evaluation of the samples tested immediately after harvest. The highest values were obtained in the 4th week after harvest. The changes were dependent on wheat cultivar. However, in the case of the assessment of the rheological characteristics of the dough (except point C4) they were not statistically significant.
    Źródło:
    Polish Journal of Agronomy; 2021, 47; 58-67
    2081-2787
    Pojawia się w:
    Polish Journal of Agronomy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Content of glycogen and trehalose and activity of alpha-amylase and trehalase in Galleria mellonella larvae infected with entomopathogenic nematodes Steinernema affinis and S. feltiae
    Autorzy:
    Zoltowska, K
    Lopienska-Biernat, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/839414.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Galleria mellonella
    Steinernema feltiae
    insect host
    amylase
    trehalase
    host
    parasite
    trehalose
    glycogen
    parasitology
    alpha-amylase
    nematode
    larva
    Steinernema affinis
    entomopathogenic nematode
    Opis:
    Introduction. The influence of infection with two species of entomopathogenic nematodes of Steinernematidae family on metabolism of glycogen and trehalose of the host was studied. Material and methods. Last instar larvae (L₇) of Galleria mellonella were experimentally infected with Steinernema affinis and S. feltiae. At 6, 12, 18 and 24 h after infection concentrations of trehalose and glycogen as well as activity of trehalase and α-amylase were determined. Results. The content of glycogen was lower in insects infected with S. feltiae than in the controls and animals infected with S. affinis. The content of trehalose was higher in insects from both infected groups than in the controls. Its concentration was slightly higher in larvae infected with S. affinis than in those infected with S. feltiae. The activity of α-amylase after infection with S. affinis was low. It was significantly higher in insects infected with S. feltiae. In animals of both infected groups, following a significant reduction at 6 h, the activity of trehalase remained at a similar level, higher than in the controls. In the paper the effects of infection with (i) different species of entomopathogenic nematodes and (ii) the importance of the developmental stage of the insect-host for changes in its metabolism of glycogen and trehalose were discussed.
    Źródło:
    Annals of Parasitology; 2006, 52, 2
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Content of glycogen and trehalose and activity of alpha-amylase and trehalase in Galleria mellonella larvae infected with entomopathogenic nematodes Steinernema affinis and S. feltiae
    Autorzy:
    Żółtowska, K.
    Łopieńska-Biernat, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2144330.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Galleria mellonella
    Steinernema feltiae
    insect host
    amylase
    trehalase
    host
    parasite
    trehalose
    glycogen
    parasitology
    alpha-amylase
    nematode
    larva
    Steinernema affinis
    entomopathogenic nematode
    Opis:
    Introduction. The influence of infection with two species of entomopathogenic nematodes of Steinernematidae family on metabolism of glycogen and trehalose of the host was studied. Material and methods. Last instar larvae (L₇) of Galleria mellonella were experimentally infected with Steinernema affinis and S. feltiae. At 6, 12, 18 and 24 h after infection concentrations of trehalose and glycogen as well as activity of trehalase and α-amylase were determined. Results. The content of glycogen was lower in insects infected with S. feltiae than in the controls and animals infected with S. affinis. The content of trehalose was higher in insects from both infected groups than in the controls. Its concentration was slightly higher in larvae infected with S. affinis than in those infected with S. feltiae. The activity of α-amylase after infection with S. affinis was low. It was significantly higher in insects infected with S. feltiae. In animals of both infected groups, following a significant reduction at 6 h, the activity of trehalase remained at a similar level, higher than in the controls. In the paper the effects of infection with (i) different species of entomopathogenic nematodes and (ii) the importance of the developmental stage of the insect-host for changes in its metabolism of glycogen and trehalose were discussed.
    Źródło:
    Wiadomości Parazytologiczne; 2006, 52, 2; 103-107
    0043-5163
    Pojawia się w:
    Wiadomości Parazytologiczne
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Starch-iodine assay method underestimates alpha-amylase inhibitory potential of antioxidative compounds and extracts
    Autorzy:
    Ononamadu, C.J.
    Ezeigwe, O.C.
    Owolarafe, T.A.
    Ihegboro, G.O.
    Lawal, T.A.
    Salawu, K.
    Umeoguaju, U.F.
    Aminu, I.
    Powiązania:
    https://bibliotekanauki.pl/articles/2096980.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    α-amylase inhibition
    starch-iodine
    diabetes mellitus
    limitations
    decolorization
    Opis:
    Starch-iodine assay method for the determination of α-amylase activity is also used in screening extracts for α-amylase inhibitors. However, there are indications that this method may not be appropriate for screening some classes of compounds or plant extracts. The present study investigated the limitation(s) of this method in screening plant extracts/compounds for α-amylase inhibition. A crude methanol extract (CR) of Dacryodes edulis, its solvent fractions (ethyl acetate (EA), aqueous methanol (AM), and hexane (HX)), quercetin (QC), and benzoic acid (BA) were used for this study. The phytochemical content and antioxidant activity were screened spectrophotometrically. α-Amylase inhibition (expressed in percentage and as IC50) was determined by starch-iodine method approach I and II (ST-ID I and ST-ID II, respectively) and dinitrosalicylic acid (DNSA) as the control method. The results showed that the extracts/compounds (AM, EA, and QC) with significantly high polyphenolic content, antioxidant activity, and starch-iodine complex decolorization effect yielded contrary results of α-amylase inhibition when the results of ST-ID I and II methods were compared to that of the DNSA method. The other test samples (CR, HX, and BA) yielded similar results for all the three methods. The result also showed the decolorization (%) of starch-iodine complex by the test samples correlated significantly (r = 0.877, P < 0.05) with DPPH reduction (%). In conclusion, the present study showed that the starch-iodine method is not appropriate for screening antioxidative extracts/compounds for α-amylase inhibitors – they decolorize the assay reagent in a manner similar to DPPH reduction and hence confound the result.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 1; 45-54
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Screening and characterization of thermo-active enzymes of biotechnological interest produced by thermophilic Bacillus isolated from hot springs in Tunisia
    Autorzy:
    Thebti, Wajdi
    Riahi, Yosra
    Gharsalli, Rawand
    Belhadj, Omrane
    Powiązania:
    https://bibliotekanauki.pl/articles/1038787.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    temperature
    amylase
    protease
    cellulase
    xylanase
    mannanase
    Geobacillus
    Tunisian hot springs
    Opis:
    As part of the contribution to the global efforts in research of thermostable enzymes being of industrial interest, we focus on the isolation of thermophilic bacteria from Tunisian hot springs. Among the collection of 161 strains of thermophilic Bacillus isolated from different samples of thermal water in Tunisia, 20% are capable of growing at 100°C and the rest grow at 70°C or above. Preliminary activity tests on media supplemented with enzyme-substrates confirmed that 35 strains produced amylases, 37 - proteases, 43 - cellulases, 31 - xylanases and 37 - mannanases. The study of the effect of temperature on enzyme activity led to determination of the optimal temperatures of activities that vary between 60 and 100°C. Several enzymes were active at high temperatures (80, 90 and 100°C) and kept their activity even at 110°C. Several isolated strains producing enzymes with high optimal temperatures of activity were described for the first time in this study. Both strains B62 and B120 are producers of amylase, protease, cellulase, xylanase, and mannanase. The sequencing of 16S DNA identified isolated strains as Geobacillus kaustophillus, Aeribacillus pallidus, Geobacillus galactosidasus and Geobacillus toebii.
    Źródło:
    Acta Biochimica Polonica; 2016, 63, 3; 581-587
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    DIAGNOSTIC POTENTIAL OF SELECTED SALIVARY PROTEOMICS FOR AUTONOMIC NERVOUS SYSTEM ACTIVITY ASSESSMENT
    Autorzy:
    Dobrek, Lukasz
    Powiązania:
    https://bibliotekanauki.pl/articles/2137800.pdf
    Data publikacji:
    2020-05-14
    Wydawca:
    Fundacja Edukacji Medycznej, Promocji Zdrowia, Sztuki i Kultury Ars Medica
    Tematy:
    saliva
    vasoactive intestinal peptide
    neuropeptide Y
    chromogranin A
    α-amylase
    Opis:
    The clinical assessment of autonomic nervous system (ANS) functioning, enabling the diagnosis of autonomic neuropathy present in the course of many diseases, is currently based on performing simple cardiovascular reflexes (Ewing tests), analyzing heart rate variability (HRV) or heart rate turbulence (HRT), examining skin sweating or recording neurophysiological tests (e.g. microneurography). Laboratory assessment of ANS function is very scarce and practically only includes the plasma assessment of noradrenaline as a surrogate for the biochemical indicator of sympathetic activity. Recently, the possibility of evaluation of selected compounds present in saliva as laboratory markers of not only oral diseases but also systemic diseases has been raised. This work focuses on a brief description of the anatomy and physiology of the salivary glands and describes the formation of saliva, its composition and the use of this bodily fluid in laboratory diagnostics. In addition, the paper specifically discusses the possibility of determining selected compounds that are considered to reflect autonomic activity. A review of the literature indicates primarily four proteomics: two neuropeptides (vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) that are co-transmitters in autonomic fibers, chromogranin A, a synaptic vesicle protein and α-amylase, a hydrolytic enzyme pre-digesting carbohydrates in the oral cavity. These are currently the most widely investigated agents for their usefulness as laboratory markers of ANS activity.
    Źródło:
    Acta Neuropsychologica; 2020, 18(2); 285-303
    1730-7503
    2084-4298
    Pojawia się w:
    Acta Neuropsychologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Core structure of flavonoids precursor as an antihyperglycemic and antihyperlipidemic agent: an in vivo study in rats
    Autorzy:
    Najafian, Mahmoud
    Ebrahim-Habibi, Azadeh
    Yaghmaei, Parichehreh
    Parivar, Kazem
    Larijani, Bagher
    Powiązania:
    https://bibliotekanauki.pl/articles/1040323.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    diabetes
    hyperglycemia
    trans-chalcone
    hyperlipidemia
    benzylideneacetophenone
    alpha-amylase inhibitor
    Opis:
    trans-Chalcone is the core structure of naringenin chalcone, located halfway in the biosynthesis pathway of flavonoids. Flavonoids have been reported as mammalian alpha-amylase inhibitors, a property which could be useful in the management of postprandial hyperglycemia in diabetes and related disorders. As a mammalian alpha-amylase inhibitor in vitro, the putative beneficial effect of trans-chalcone on diabetes was tested in a streptozotocin-induced rat model of diabetes type 1, and the results analyzed with commonly used statistical methods. Significant reduction of blood glucose levels and beneficial effect on dyslipidemia were observed in diabetic rats, as well as reduction of disturbing consequences of diabetes such as high urine volume and water intake. trans-chalcone was observed to have a weight loss-inductive effect, alongside with a reduction in food intake, which is suggestive of a therapeutic potential of this compound in overweight and obese patients.
    Źródło:
    Acta Biochimica Polonica; 2010, 57, 4; 553-560
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Purification and characterization of α-amylases from the intestine and muscle of Ascaris suum (Nematoda).
    Autorzy:
    Żółtowska, Krystyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1044107.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Ascaris suum
    α-amylase
    starch digestion
    glycogen metabolism
    nematode
    Opis:
    α-Amylase (EC 3.2.1.1) was purified from the muscle and intestine of the parasitic helminth of pigs Ascaris suum. The enzymes from the two sources differed in their properties. Isoelectric focusing revealed one form of α-amylase from muscles with pI of 5.0, and two forms of amylase from intestine with pI of 4.7 and 4.5. SDS/PAGE suggested a molecular mass of 83 kDa and 73 kDa for isoenzymes of α-amylases from intestine and 59 kDa for the muscle enzyme. α-Amylase from intestine showed maximum activity at pH 7.4, and the enzyme from muscle at pH 8.2. The muscle enzyme was more thermostabile than the intestinal α-amylase. Both the muscle and intestine amylase lost half of its activity after 15 min at 70°C and 50°C, respectively. The Km values were: for muscle amylase 0.22 μg/ml glycogen and 3.33 μg/ml starch, and for intestine amylase 1.77 μg/ml glycogen and 0.48 μg/ml starch. Both amylases were activated by Ca2+ and inhibited by EDTA, iodoacetic acid, p-chloromercuribenzoate and the inhibitor of α-amylase from wheat. No significant differences were found between the properties of α-amylases from parasites and from their hosts.
    Źródło:
    Acta Biochimica Polonica; 2001, 48, 3; 763-774
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Bioethanol Production from Chlorella Pyrenoidosa by Using Enzymatic Hydrolysis and Fermentation Method
    Autorzy:
    Yerizam, Muhammad
    Jannah, Asyeni Miftahul
    Aprianti, Nabila
    Powiązania:
    https://bibliotekanauki.pl/articles/2202327.pdf
    Data publikacji:
    2023
    Wydawca:
    Polskie Towarzystwo Inżynierii Ekologicznej
    Tematy:
    Chlorella pyrenoidosa
    hydrolysis
    enzyme
    alpha-amylase
    glucose
    fermentation
    bioethanol
    Opis:
    Starch can be found in microalgae, the raw material for the third generation of bioethanol production. One of them is C. pyrenoidosa. This study was conducted to analyze the effect of α-amylase enzyme concentration on the glucose contents produced and the effects of fermentation time on bioethanol contents produced. The hydrolysis process of this study was conducted using an α-amylase enzyme produced by A. niger. Several analyses in this research were carried out, including the analysis of enzyme activity using the Fuwa method, the analysis of glucose levels from enzymatic hydrolysis using the DNS method, and the analysis of bioethanol contents using the density method and GC-MS. The highest glucose content was 0.67 mg/mL, which was obtained from the addition of 40% (v/w) α-amylase enzyme, and the yield of bioethanol content from the sample treated 40% (v/w) α-amylase enzyme and fermented for 9 days was the optimum, which produced 28.07% of bioethanol content.
    Źródło:
    Journal of Ecological Engineering; 2023, 24, 1; 34--40
    2299-8993
    Pojawia się w:
    Journal of Ecological Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Production of amylase by the intestinal microflora of cultured freshwater fishes (Oreochromis niloticus and Clarias gariepinus) rared localy in Calabar, south Nigeria
    Autorzy:
    Martin, M. F.
    Okpo, E. A.
    Andy, I. E.
    Powiązania:
    https://bibliotekanauki.pl/articles/1076792.pdf
    Data publikacji:
    2019
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Amylase
    Clarias gariepinus
    Cultured Fresh Water Fishes
    Intestinal Microflora
    Oreochromis niloticus
    Opis:
    This study focused on determining the amylase-producing ability of the intestinal microbes in cultured fresh water fishes – Oreochromis niloticus and Clarias gariepinus. The bacterial isolates were identified on the basis of standard cultural, morphological and biochemical characteristics. The amylase production ability of the bacterial isolates was determined using starch agar. The mean viable count of the intestinal microbes ranged from 1.2 × 105 CFU/ml to 7.1 × 105 CFU/ml for tilapias (Oreochromis niloticus) and from 2.0 × 104 CFU/ml to 8.9 × 104 CFU/ml for catfishes (Clarias gariepinus). Staphylococcus and Micrococcus were predominant for both tilapias and catfishes. Out of 24 isolates, 21 were amylase producers. These included the following bacteria genera: Bacillus, Micrococcus, and Staphylococcus. These results strongly suggest that intestinal microbes play a pivotal role in the digestion of starch in cultured freshwater fishes and should be explored for industrial amylase production.
    Źródło:
    World News of Natural Sciences; 2019, 23; 13-23
    2543-5426
    Pojawia się w:
    World News of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The relationship of biochemical parameters and radiological parameters in the evaluation of the clinical severity of acute pancreatitis in the emergency department – a retrospective analysis
    Autorzy:
    Tortum, Fatma
    Tekin, Erdal
    Aydın, Fahri
    Özdal, Emine
    Tatlısu, Kübra
    Powiązania:
    https://bibliotekanauki.pl/articles/25102374.pdf
    Data publikacji:
    2023-06-30
    Wydawca:
    Uniwersytet Rzeszowski. Wydawnictwo Uniwersytetu Rzeszowskiego
    Tematy:
    acute pancreatitis
    amylase
    computed tomography severity index
    emergency department
    lipase
    Opis:
    Introduction and aim. Computed tomography severity index (CTSI) and Balthazar score are among the most frequently used scorings in the determination of severe acute pancreatitis. The primary purpose of this study is evaluation of the effects of biochemical parameters, Balthazar score and CTSI on mortality in acute pancreatitis. At the same time, correlations with biochemical parameters, CTSI and Balthazar score were evaluated in patients with AP. Material and methods. In this study, the amylase, lipase, CRP, and procalcitonin values of patients diagnosed with acute pancreatitis were retrospectively recorded. Contrast-enhanced computed tomography (CECT) images obtained at the time of presentation to the emergency department or within seven days of admission were re-evaluated by two radiologists. The CTSI scores and Balthazar scores of the patients were calculated. Results. The study included 240 patients. The amylase level of the patients was positively correlated with the Balthazar score at a statistically significant level (R=0.189, p=0.003). In addition, the relationship between pancreatic scoring systems and mortality, the AUC value for CTSI was 0.9 (95% CI: 0.826-0.973) and was higher than other scoring systems. Conclusion. CTSI had better performance in the prediction of mortality in patients with acute pancreatitis.
    Źródło:
    European Journal of Clinical and Experimental Medicine; 2023, 2; 277-282
    2544-2406
    2544-1361
    Pojawia się w:
    European Journal of Clinical and Experimental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Physical factors affecting the production of amylase from Bacillus species isolated from natural environment
    Wpływ czynników fizycznych na aktywność amylolityczną Bacillus sp. wyizolowanych ze środowiska naturalnego
    Autorzy:
    Grata, K.
    Nabrdalik, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/389748.pdf
    Data publikacji:
    2014
    Wydawca:
    Towarzystwo Chemii i Inżynierii Ekologicznej
    Tematy:
    Bacillus cereus
    Bacillus mycoides
    amylase
    physical factor
    amylazy
    czynniki fizyczne
    Opis:
    The influence of different factors on amylolytic activity Bacillus cereus (2 strains) and Bacillus mycoides (2 strains), isolated from soil samples and water of Turawa Lake has been studied. Effect of physiological (pH of reaction mixture in the range of 4.0–8.0, temperature of reaction from 30 to 60 oC) and nutritional parameters (the carbon sources and they concentration in medium) were examined spectophotometricaly for their effect on amylase production. The results obtained showed, that in view of all studied factors the least favorable value of pH was 5.0, while in most cases the most favourable for the process of amylase production were following values 7.0 and 8.0. Moreover, it has been stated that the lowest amount of amylase was noted at 30 oC, and the highest at 60 oC. In case of amylolytic activity, in view of all temperatures the most effective strain was B. mycoides A134 whereas the least B. mycoides G3. The best results of amylase production have been achieved for all remaining strains in two cases – on maltose and glucose medium at all tested temperatures. Additionally, soil occurring strains preferred lower concentration of potato starch (ie 1 %) when compared with water strains which favoured higher concentration (5 %).
    Celem badań była ocena wpływu różnych czynników na aktywność amylolityczną Bacillus cereus (2 szczepy) i Bacillus mycoides (2 szczepy) wyizolowanych z gleby i Jeziora Turawskiego. Metodą spektrofotometryczną badano wpływ pH mieszaniny reakcyjnej w zakresie od 5.0 do 8.0 i temperatury w zakresie od 30 oC do 60 oC oraz źródła węgla i jego koncentracji w podłożu na poziom produkowanych amylaz. Stwierdzono, iż najgorszą wartością pH do syntezy amylaz było 5.0, natomiast najlepszą, w większości przypadków pH 7.0. Uwzględniając zakres badanych temperatur, najwyższą aktywność uzyskano w temp. 60 oC, natomiast najniższą w temp. 30 oC dla wszystkich testowanych szczepów. Najbardziej aktywnym szczepem był B. mycoides A 134, natomiast najmniej B. mycoides G3.
    Źródło:
    Ecological Chemistry and Engineering. A; 2014, 21, 1; 51-60
    1898-6188
    2084-4530
    Pojawia się w:
    Ecological Chemistry and Engineering. A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effectiveness of hemoperfusion in treatment of patients with non-biliary moderately severe pancreatitis
    Skuteczność zastosowania hemoperfuzji w leczeniu pacjentów z ostrym martwiczym zapaleniem trzustki
    Autorzy:
    Oliynyk, O.
    Ślifirczyk, A.
    Pereviznyk, B.
    Kushnarov, W.
    Powiązania:
    https://bibliotekanauki.pl/articles/2052946.pdf
    Data publikacji:
    2018
    Wydawca:
    Akademia Bialska Nauk Stosowanych im. Jana Pawła II w Białej Podlaskiej
    Tematy:
    acute pancreatitis
    hemoperfusion
    amylase
    procalcitonin
    ostre zapalenie trzustki
    hemoperfuzja
    amylaza
    prokalcytonina
    Opis:
    Background. Acute pancreatitis morbidity has been rising annually all around the world. In case of acute necrotic pancreatitis, the mortality reaches 40% in the majority of hospitals. The therapy is effective if the efferent methods involve complex therapy of this disease. One such method is hemoperfusion, i.e. extracorporeal blood purification, which is widely used in toxicology. The authors of the article used hemoperfusion in a complex therapy in patients with severe acute pancreatitis. The following research presents an evaluation of the results of hemoperfusion used in a complex therapy of patients with acute pancreatitis. Material and methods. The study involved 38 patients with acute non-biliary moderately severe pancreatitis who were treated at an intensive care unit of Ternopil University Hospital in Ukraine. 18 patients were treated following the protocol for treatment of acute pancreatitis. In 20 patients, this treatment was additionally combined with hemoperfusion. We determined the levels of amylase, diastase, procalcitonin, bilirubin, malonic dialdehyde, and diene conjugates in blood serum and the level of leukocytes in the blood. Results. The levels of procalcitonin, amylase, bilirubin, leukocytes number, malonic dialdehyde, and diene conjugates were stabilised in patients of both groups, but in those who received hemoperfusion demonstrated much better results. These were manifested by significantly better levels of the amylase, bilirubin, creatinine, urea, procalcitonin, malonic dialdehyde, and diene conjugates in patients who received hemoperfusion (p< 0.005) than in the other group undergoing standard drug therapy. Conclusions. Hemoperfusion can be used as an effective method in the complex treatment of patients with acute pancreatitis.
    Wprowadzenie. Corocznie na całym świecie obserwuje się w zrost zachorowalności na ostre zapalenie trzustki. W przypadku ostrego martwiczego zapalenia trzustki śmiertelność w większości szpitali osiąga 40%. Terapia jest skuteczna, jeśli metody odprowadzające obejmują kompleksową terapię tej choroby. Jedną z takich metod jest hemoperfuzja, tj. pozaustrojowa hemodializa krwi, szeroko stosowane w toksykologii. Autorzy artykułu zastosowali hemoperfuzję w kompleksowej terapii u pacjentów z ciężkim ostrym zapaleniem trzustki. Poniższe badania przedstawiają ocenę wyników hemoperfuzji stosowanej w kompleksowej terapii pacjentów z ostrym zapaleniem trzustki. Materiał i metody. W badaniu wzięło udział 38 pacjentów z ostrym, niezłośliwym i umiarkowanie ciężkim zapaleniem trzustki; leczonych w oddziale intensywnej opieki medycznej szpitala uniwersyteckiego na Ukrainie. Zgodnie z protokołem leczenia ostrego zapalenia trzustki leczono18 pacjentów. U 20 pacjentów leczenie to połączono dodatkowo z hemoperfuzją. Określono poziom amylazy, diastazy, prokalcytoniny, bilirubiny, dialdehydu malonowego i koniugatów dienowych w surowicy krwi oraz poziom leukocytów we krwi. Wyniki. Poziom prokalcytoniny, amylazy, bilirubiny, liczby leukocytów, dialdehydu malonowego i koniugatów dienowych były stabilizowane u pacjentów z obu grup, ale u tych pacjentów, u których włączono hemoperfuzję, wyniki były zdecydowanie lepsze. Przejawiały się one znacznie wyższym poziomem amylazy, bilirubiny, kreatyniny, mocznika, prokalcytoniny, dialdehydu malonowego i dienowych koniugatów u pacjentów, którym włączono hemoperfuzję hemoperfuzję (p <0,005) niż w drugiej grupie poddawanej standardowej terapii lekowej. Wnioski. Hemoperfuzja może być stosowana jako skuteczna metoda w kompleksowym leczeniu pacjentów z ostrym zapaleniem trzustki.
    Źródło:
    Health Problems of Civilization; 2018, 12, 3; 204-208
    2353-6942
    2354-0265
    Pojawia się w:
    Health Problems of Civilization
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Gullo’s syndrome – case report
    Autorzy:
    Rycyk, A.
    Furtak, P.
    Madro, A.
    Kasztelan-Szczerbinska, B.
    Cichoz-Lach, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/2098199.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Gullo's syndrome
    pancreatic enzyme
    lipase
    amylase
    carcinoembryonic antigen
    carbohydrate antigen
    serine protease
    ultrasonography
    Opis:
    Benign pancreatic hyperenzymemia (BPH) or Gullo’s Syndrome is a persistent elevation of pancreatic enzymes activity, observed for at least one year, with no pancreatic disorder. This diagnosis is extremely important because it allows us to avoid many unnecessary examinations performed during the diagnostic process. We present a case of a 25-year-old man who was admitted for recurrent elevated lipase and amylase serum values over a time period of 2 years who presented with non-specific abdominal complaints. Interestingly, his routine tests showed sustained elevated serum amylase and lipase activity. He was intensively diagnosed due to pancreatic hyperenzymemia, but no pancreatic disease was detected. The observation lasted two years. The serum lipase and serum amylase levels continued to increase after that time. This diagnosis requires attention because BPH can be the first symptom of pancreatic cancer.
    Źródło:
    Journal of Pre-Clinical and Clinical Research; 2020, 14, 4; 117-119
    1898-2395
    Pojawia się w:
    Journal of Pre-Clinical and Clinical Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Germination, alpha-, beta-amylase and total dehydrogenase activities of Amaranthus caudatus seeds under water stress in the presence of ethephon or gibberellin A3
    Autorzy:
    Bialecka, B
    Kepczynski, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/19888.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Amaranthus caudatus
    seed
    germination
    alpha-amylase activity
    beta-amylase activity
    dehydrogenase activity
    water stress
    ethylene
    gibberellin A3
    polyethylene glycol
    ethephon
    water deficit
    laboratory condition
    plant
    environmental stress
    Opis:
    Amaranthus caudatus L. seed germination was studied under different levels of water deficit induced by PEG 6000 in laboratory conditions. PEG at osmotic potentials -0.2 to -0.3 MPa at 24°C in darkness delayed germination and reduced final germination percentage. PEG solutions at osmotic potential lower than -0.3 MPa almost totally blocked seed germination. Ethephon was much more effective than GA3 in reversing PEG-caused inhibition of A. caudatus seed germination. PEG decreased α-amylase activity after 14 h incubation. It decreased β-amylase activity after 14 and 20 h, and caused an increase in total dehydrogenase activity only after 20 h of incubation. Unlike GA3, ethephon increased α-amylase activity in seeds after 12 and 14 h of incubation under water deficit. After 20 h of incubation there was no difference in α-amylase activity in any of the treatments. Neither ethephon nor GA3 affected the activity of β-amylase and dehydrogenase.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2010, 52, 1; 7-12
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Involvement of ROS and its scavenging enzymes in the response of dormant Avena fatua L. caryopses to karrikinolide (KAR1)
    Autorzy:
    Cembrowska-Lech, D.
    Kepczynski, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/80050.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    reactive oxygen species
    scavenging enzyme
    Avena fatua
    caryopsis
    germination
    dehydrogenase activity
    amylase
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro α-glucosidase and α-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide
    Autorzy:
    Subramanian, Rammohan
    Asmawi, M
    Sadikun, Amirin
    Powiązania:
    https://bibliotekanauki.pl/articles/1040761.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    α-glucosidase
    α-amylase
    Andrographis paniculata
    andrographolide
    peak blood glucose
    post prandial hyperglycaemia.
    Opis:
    There has been an enormous interest in the development of alternative medicines for type 2 diabetes, specifically screening for phytochemicals with the ability to delay or prevent glucose absorption. The goal of the present study was to provide in vitro evidence for potential inhibition of α-glucosidase and α-amylase enzymes, followed by a confirmatory in vivo study on rats to generate a stronger biochemical rationale for further studies on the ethanolic extract of Andrographis paniculata and andrographolide. The extract showed appreciable α-glucosidase inhibitory effect in a concentration-dependent manner (IC50=17.2±0.15 mg/ml) and a weak α-amylase inhibitory activity (IC50=50.9±0.17 mg/ml). Andrographolide demonstrated a similar (IC50=11.0±0.28 mg/ml) α-glucosidase and α-amylase inhibitory activity (IC50=11.3±0.29 mg/ml). The positive in vitro enzyme inhibition tests paved way for confirmatory in vivo studies. The in vivo studies demonstrated that A. paniculata extract significantly (P<0.05) reduced peak blood glucose and area under curve in diabetic rats when challenged with oral administration of starch and sucrose. Further, andrographolide also caused a significant (P<0.05) reduction in peak blood glucose and area under the curve in diabetic rats. Hence α-glucosidase inhibition may possibly be one of the mechanisms for the A. paniculata extract to exert antidiabetic activity and indicates that AP extract can be considered as a potential candidate for the management of type 2 diabetes mellitus.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 2; 391-398
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Interaction between larval alpha-amylase of the tomato leaf miner, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) and proteinaceous extracts from plant seeds
    Autorzy:
    Esmaeily, M.
    Bandani, A.R.
    Powiązania:
    https://bibliotekanauki.pl/articles/65574.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    interaction
    larva
    alpha-amylase
    tomato leafminer
    Tuta absoluta
    Lepidoptera
    Gelechiidae
    plant extract
    seed
    plant seed
    Opis:
    The tomato leaf miner, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae), is one of the most destructive pest of solanaceae and it prefers tomato (Solanum lycopersicum L.). The aim of the current study was to investigate the effects of a wide range of seed proteinaceous extracts from different plant families against T. absoluta α-amylase activity. The effect of pH on the inhibitory activity of seed extracts showed that seed extracts of amaranth along with a wheat cultivar (Alvand, Aflak, Sarvdasht, Alborz, and Kavir) produced more than a 50% inhibition of the insect amylase. Aflak wheat seed extract at 10 μg, inhibited 81% of the insect amylase. This percent was the highest inhibition achieved. The other proteinaceous seed extracts had a lower effect on the enzymatic activity. Probit analysis showed that Aflak, Kavir, Alborz, Alvand, Sarvdasht, and amaranth inhibited the amylase activity with an I50 of 1.94, 3.24, 3.46, 3.31, 4.97, and 15.39 μg, respectively. The effect of pH on the inhibition of the α-amylase showed the highest inhibition of Amaranth and wheat, at a pH value of 8.0, which corresponds to the pH of the insect’s gut. Gel electrophoresis assays confirmed the spectrophotometric assays showing that the α-amylase of the insect gut was affected by the presence of the seed extracts. In the gel assay, a high concentration (14 μg protein) of amaranth proteinaceous seed extract greatly decreased the intensity of the α-amylase band. A high concentration of the Aflak wheat cultivar (10 μg protein) caused the disappearance of the amylase band in the gel. Thus, it is concluded that the physiochemical environment of the insect gut affects the interaction between digestive α-amylase and the metabolites. The experiments showed that seed proteinaceous extracts from non-host plant species, produced more inhibition of the insect amylase when compared to the host plant species. It appears that with evolution, adaptation took place so that insect/s could overcome the plant metabolites.
    Źródło:
    Journal of Plant Protection Research; 2015, 55, 3
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wheat seedlings as affected by Mg(NO3)2 and ZnSO4 priming treatments
    Autorzy:
    Singhal, Rajesh Kumar
    Bose, Bandana
    Powiązania:
    https://bibliotekanauki.pl/articles/1030251.pdf
    Data publikacji:
    2020
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Mean germination time
    Seed priming
    Seedling vigour
    Soluble and insoluble sugar content
    Triticum aestivum
    α-amylase activity
    Opis:
    Seed is the most crucial and prime input in agriculture, and the seed must be of high quality. Seed priming is one of the reliable and cost-effective strategies to enhance seed germination and seedling establishment in crops. This study was conducted to determine the effects of seed priming with Mg(NO3)2 (S1) and ZnSO4 (S2) in two wheat (Triticum aestivum L.) varieties (HUW-468 and HUW-510) on germination and seedling growth. Varied levels of Mg(NO3)2 and ZnSO4 from C0 to C11 were used for the present study and number of seed germinated, seedling establishment and biochemical parameters were recorded. From the study of germination %, mean germination time (MGT), mean germination rate (MGR), coefficient of velocity of germination (CVG), germination rate index (GRI) (% / day), germination index (GI), seedling vigor (SV), shoot, root and seedling length, α-amylase activity, soluble and insoluble sugar content and protein content, it was revealed that 7.5 mM of Mg(NO3)2 and 0.05 mM of ZnSO4 were the best treatments in improving seedling establishment, performance and biochemical status of wheat seed. Further, this analysis also reveals a strong correlation between the seedling establishment and the activity of -amylase and protein content.
    Źródło:
    World Scientific News; 2020, 144; 13-29
    2392-2192
    Pojawia się w:
    World Scientific News
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wybrane czynniki psychiczne a częstość wymiotów w przebiegu żarłoczności psychicznej oraz ich związek ze zmianami stężeń amylazy w surowicy i w moczu
    Correlation of selected mental factors with frequency of vomiting in the course of bulimia nervosa and their association with altered amylase level in serum and urine
    Autorzy:
    Kostulski, Adrian
    Pawełczyk, Tomasz
    Rabe-Jabłońska, Jolanta
    Powiązania:
    https://bibliotekanauki.pl/articles/945503.pdf
    Data publikacji:
    2008
    Wydawca:
    Medical Communications
    Tematy:
    amylase
    amylaza
    anxiety
    body image
    bulimia nervosa
    depresja
    depression
    hostility
    lęk
    obraz ciała
    wrogość
    żarłoczność psychiczna
    Opis:
    One of the most health- and sometimes also life-threatening symptoms of bulimia nervosa is vomiting. It may lead to severe complications, e.g. electrolyte imbalance, metabolic alkalosis, hyperchloremia, hypokalemia, metabolic disturbances, heart arrhythmia, vitamin deficit, damage of posterior wall of pharynx and oesophagus, erosion of dental enamel. Detection of a specific predictor of unfavourable consequences of the disease might help to define a group of patients more at risk of developing uncontrollable vomiting in the course of bulimia nervosa, as well as to provide a means of monitoring frequency of vomiting in non-cooperative patients. The aim of this study was to explore correlations between frequency of vomiting in the course of bulimia nervosa and selected variables, e.g. symptoms of depression, anxiety, hostility, dissatisfaction with own body image and elevated levels of amylase in serum and urine. Using a specially designed questionnaire, Hamilton Depression Scale, Hamilton Anxiety Scale, Buss-Durkee Hostility Inventory and Body Image Questionnaire, we studied 55 patients (54 females and 1 male) with a diagnosis of bulimia nervosa (acc. to ICD-10), treated at the out-patients clinic (52 persons) and institutionalised (3 persons) since 2003 thru 2005. Each participant of the study was tested for serum and urine amylase level. Study protocol has been approved by Bioethical Committee. In the study group we noticed a significant positive correlation between frequency of vomiting and severity of anxiety and hostility and amylase level in serum and urine. Furthermore, a significant positive correlation was noticed between amplitude of body mass fluctuations, severity of hostility and amylase level in serum and urine. No associations were found between frequency of vomiting, presence of depression and dissatisfaction with own body.
    Jednym z najbardziej niebezpiecznych dla zdrowia, a często i dla życia objawów żarłoczności psychicznej (ZP) są wymioty. Mogą one prowadzić do poważnych powikłań, takich jak: zaburzenia elektrolitowe, zasadowica metaboliczna, hiperchloremia, hipokaliemia, zaburzenia metaboliczne, zaburzenia rytmu serca, niedobory witamin, uszkodzenie tylnej ściany gardła i przełyku, erozja szkliwa zębów. Znalezienie predyktora niekorzystnych konsekwencji choroby mogłoby pomóc w wyodrębnieniu pacjentek bardziej zagrożonych ryzykiem wymiotów w przebiegu ZP, a także dałoby szansę monitorowania częstości wymiotów u pacjentek niewspółpracujących. Celem badania było ustalenie związku pomiędzy częstością wymiotów w przebiegu ZP a wybranymi zmiennymi, takimi jak: obecność objawów depresji, lęku, wrogości, niezadowolenia z obrazu własnego ciała oraz podwyższonych stężeń amylazy w surowicy krwi i moczu. Za pomocą specjalnie opracowanego kwestionariusza oraz Skali Depresji Hamiltona, Skali Lęku Hamiltona, Kwestionariusza Wrogości Bussa-Durkee, Kwestionariusza Obrazu Ciała przebadano 55 pacjentów (54 kobiety i 1 mężczyznę) z rozpoznaniem ZP (wg ICD-10), leczonych w poradni (52 osób) oraz w oddziale (3 osoby) w latach 2003-2005. U każdego uczestnika badania oznaczono stężenia amylazy we krwi i w moczu. Na przeprowadzenie badania uzyskano zgodę Komisji Bioetycznej. W grupie badanej stwierdzono istotną dodatnią korelację pomiędzy częstością wymiotów a nasileniem objawów lęku oraz wrogości oraz stężeniem amylazy w surowicy i moczu. Ustalono także występowanie istotnej dodatniej korelacji pomiędzy wielkością zmian/wahań masy ciała a nasileniem wrogości oraz stężeniem amylazy w surowicy i moczu. Nie obserwowano związku między częstością wymiotów a obecnością depresji oraz niezadowolenia z własnego ciała.
    Źródło:
    Psychiatria i Psychologia Kliniczna; 2008, 8, 2; 104-111
    1644-6313
    2451-0645
    Pojawia się w:
    Psychiatria i Psychologia Kliniczna
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic polymorphism and quantitative variation of alpha-amylases from rye endosperm
    Autorzy:
    Masojc, P
    Lapinski, M.
    Larsson-Raznikiewicz, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2048192.pdf
    Data publikacji:
    1995
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    chromosome
    rye
    Secale cereale
    colorimetric assay
    isoenzyme
    alpha-amylase
    electrophoresis
    endosperm
    isoelectric focusing
    immunoelectrophoresis
    cereal cultivar
    genetic polymorphism
    Opis:
    α-Amylase isozymes from rye endosperm were analysed by means of isoelectric focusing, polyacrylamide gel electrophoresis, immunoelectrophoresis and colorimetric assay. Α-AMY 1 (high pI) group was separated into 13 IEF bands, whereas in group α-AMY2 (low pI) 2 intensive and 6-8 faint bands were found. Two linked (2±1 cM) polymorphic loci and a single locus with two alleles encoding for α-AMY 1 and α-AMY 2 groups, respectively, were identified after genetic analysis of the IEF patterns. All α-amylase isozymes developed on PAGE, were shown to belong to α-AMY 1 group. It was demonstrated that a single PAGE isozyme corresponds to 2-4 separate IEF bands and that most of the IEF bands can be attributed to more than one PAGE isozyme. The activity of α-amylases from PAGE zone I was 2.3 times higher than the activity of zone II isozymes. A strong correlation between the activity and protein amount of particular α-AMY 1 isozymes (r=0.94) was found.
    Źródło:
    Journal of Applied Genetics; 1995, 36, 3; 197-204
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic regulation of alpha-amylase synthesis in rye [Secale cereale L.] grain
    Autorzy:
    Masojc, P
    Stojalowski, S
    Lapinski, M
    Miazga, D
    Powiązania:
    https://bibliotekanauki.pl/articles/2047279.pdf
    Data publikacji:
    1996
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    enzyme synthesis
    activity
    chromosome
    rye
    Secale cereale
    crossing
    alpha-amylase
    sprouting line
    duplicate gene
    addition line
    genetic regulation
    Opis:
    Genetic analysis of two rye interline crosses and a set of wheat/rye chromosomal addition lines was performed to reveal the mechanism underlying wide variation range of alpha-amylase activity in sound grain. The long arm of chromosome 6R was found to be responsible for increased enzyme synthesis during late stages of triticale grain maturation. Only nuclear genes seemed to control alpha-amylase activity, as reciprocal crosses between rye lines showed no maternal effects. Low enzyme activity showed complete dominance over high level of its synthesis. Segregation ratios, observed in F₂ and BC₁ crosses, indicated that recessive alleles at two independent duplicative loci underlie intensive alpha-amylase production.
    Źródło:
    Journal of Applied Genetics; 1996, 37, 2; 141-152
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Assessment of chemical composition of flour and its effect on baking characteristics of wheat
    Autorzy:
    Subda, H
    Powiązania:
    https://bibliotekanauki.pl/articles/1371393.pdf
    Data publikacji:
    1992
    Wydawca:
    Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
    Tematy:
    flour
    wheat flour
    glutenin
    food
    gliadin
    chemical composition
    high-molecular glutenin
    proteolytic enzyme
    wheat
    alpha-amylase
    baking
    protein
    total protein
    Źródło:
    Polish Journal of Food and Nutrition Sciences; 1992, 01, 2; 15-21
    1230-0322
    2083-6007
    Pojawia się w:
    Polish Journal of Food and Nutrition Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biochemical indexes of the liver functioning at the acute stage of opisthorchiasis
    Autorzy:
    Bakshtanovskaya, I.
    Stepanova, T.
    Shonin, A.
    Ponomareva, O.
    Terekhina, V.
    Powiązania:
    https://bibliotekanauki.pl/articles/837329.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    bilirubin
    gamma-glutamyltransferase
    glucose
    alanine aminotransferase
    aspartate aminotransferase
    concentration
    alkaline phosphatase
    opistchorchiasis
    alpha-amylase
    cholesterol
    liver function
    serum activity
    Źródło:
    Annals of Parasitology; 1998, 44, 3
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of thyroxin induction on enzymatic and biochemical levels in albino rats
    Autorzy:
    Hanchinalmath, J.V.
    Shivasharanappa, K.
    Roopa, T.S.
    Saisundeep, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/11503.pdf
    Data publikacji:
    2014
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    thyroxine
    alpha-amylase
    enzymatic level
    biochemical level
    albino rat
    rat
    kidney
    liver
    blood serum
    creatine phosphokinase activity
    cholesterol
    citric acid
    triglyceride
    Opis:
    The albino Wistar rats were used to investigate the effect of thyroxine administration on the activity of citric acid, α-Amylase, creatine phosphokinase (CPK), creatinine, cholesterol and triglycerides level in blood serum and citric acid in liver and kidney. Two groups of rats were taken for the experiment. One group was injected subcutaneously with 200 μl of thyroxine/50 g of body weight, the other group served as control. The animals were vivisected with anesthesia after intervals of 24, 48, 72 and 96 hours α-Amylase and CPK levels in blood; citric acid level in liver and kidney of control and thyroxine treated rats. The results showed that the α-Amylase activity remained unaltered (100 g/h/l) throughout the experiment. However, citric acid level found to be 460 mg in the liver and 415 mg in kidney of control rats. The values slightly increased to 480 and 680 mg in respective tissues of thyroxine treated animals for 24 hr. The values decreased in 48 and 72 hours of treatment. Further the values found to be increased after 96 hours of treatment. Although the thyroxine treatment altered citric acid, Creatinine, Cholesterol, Triglycerides and CPK levels in the tissues, the serum α-Amylase was not influenced by the thyroxine. The study suggests that the thyroxine acts as a regulator all the above parameters in the cellular level.
    Źródło:
    International Letters of Natural Sciences; 2014, 08, 2
    2300-9675
    Pojawia się w:
    International Letters of Natural Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Isolation and preliminary sequence characterization of beta-amylase gene promoters in rye [Secale cereale L.]
    Autorzy:
    Sadowski, J
    Rorat, T
    Cooke, R
    Delseny, M
    Powiązania:
    https://bibliotekanauki.pl/articles/2046684.pdf
    Data publikacji:
    1997
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    beta-amylase
    rye
    gene promoter
    homology
    amino acid
    DNA cloning
    prolamin
    Secale cereale
    mRNA
    embryogenesis
    endosperm
    cDNA
    inverse polymerase chain reaction
    Opis:
    The isolation of rye ß-Amy1 and ß-Amy2 gene promoters from nuclear DNA using the inverse polymerase chain reaction (IPCR) technique and characterization of their sequences are presented. The conservation of ß-amylasc coding sequences allowed for simultaneous IPCR amplification of two different promoters with primers designed on the basis of the single known cDNA sequence. Two ß-amylasc gene promoters display a low sequence similarity (47%). Beside consensus TATA and CCAAT boxes, other sequence motives common to both promoters were found. In addition, the homology of amino acid sequences of plant ß-amylases available in the database is discussed.
    Źródło:
    Journal of Applied Genetics; 1997, 38, 3; 241-251
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Plant height and yield components of inbred isogenic and F1 hybrid Rht dwarf wheats
    Autorzy:
    Flintham, J E
    Gale, M.D.
    Powiązania:
    https://bibliotekanauki.pl/articles/2044459.pdf
    Data publikacji:
    1998
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    chemical hybridizing agent
    F1 hybrid
    wheat
    plant height
    grain yield
    hybrid genotype
    heterosis
    alpha-amylase
    enzyme activity
    breeding programme
    yield component
    Rht allele
    Opis:
    Single and double-gene Rhtl, Rht2, Rht3, Rht1 + Rht2 and Rht3 + Rht2 isogenic lines of wheat in four parental rht varieties were grown in drilled yield trials at four sites in 1989. The same lines were also grown in 1988 together with hybrid genotypes from CHA (chemical hybridising agent) F₁ production plots. In the inbred lines shorter than one metre, Rht alleles reduced total shoot biomass by shortening the straw; mass of straw per unit plant height was unaffected. Highest grain yield was obtained from plant heights between 70 and 100 cm. The Rht genotype achieving this stature varied according to parent varietal height. The hybrids grown allowed comparisons between intra- and inter-varietal crosses over a range of Rht genotypes. In F₁ hybrids positive heterosis was observed for plant height, grain yield and mean grain weight. Highest yields were obtained from inter-varietal hybrids carrying one, two or three doses of Rht1 or Rht2 or one dose of Rht3. An Rht3lrht hybrid showed resistance to premature α-amylase production during grain ripening.
    Źródło:
    Journal of Applied Genetics; 1998, 39, 1; 73-83
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Oznaczanie zawartości alfa-amylazy w ziarnie pszenicy zwyczajnej na podstawie liczby opadania
    Designation of contents alpha-amylase in the grain of the normal wheat on the basis of falling the number
    Autorzy:
    Rut, J.
    Szwedziak, A.
    Kuczek, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/228905.pdf
    Data publikacji:
    2007
    Wydawca:
    Wyższa Szkoła Menedżerska w Warszawie
    Tematy:
    liczba opadania
    alfa-amylaza
    ziarno pszenicy
    enzymy
    cele chlebowe
    wartość wypiekowa
    number of falling
    alpha-amylase
    grain of wheat
    enzymes
    of bread cells
    baking value
    Opis:
    W artykule przedstawiono aktywność alfa-amylazy w ziarnie pszenicy za pomocą pomiaru liczby opadania. Pomiar liczby opadania służy do określenia stanu aktywności enzymów w ziarnie, określa przydatność do wypieku - mąki uzyskanej z tego ziarna, a jednocześnie stan fizjologiczny ziarna, informujący o jego trwałości w przechowywaniu.
    In the article an activity was introduced alpha-amylase In a grain of wheat behind the help of falling the measurement of the number. The measurement of falling the number is serving for defining the state of the activity of enzymes in the grain, a usefulness for the baking is determining - of flour gotten of this grain, and at the same time the physiological state of the modicum, informing of its permanence in keeping.
    Źródło:
    Postępy Techniki Przetwórstwa Spożywczego; 2007, 2; 23-25
    0867-793X
    2719-3691
    Pojawia się w:
    Postępy Techniki Przetwórstwa Spożywczego
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zmiany właściwości wypiekowych mąki żytniej pod wpływem dodatku alfa-amylazy
    Changes in baking properties of rye flour impacted by alpha-amylase addition
    Autorzy:
    Szafranska, A.
    Slowik, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/35734.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Instytut Agrofizyki PAN
    Tematy:
    piekarstwo
    maka zytnia
    wartosc wypiekowa
    dodatki do zywnosci
    alfa-amylazy
    ciasto
    chleb
    pieczywo
    jakosc
    baking
    rye flour
    baking value
    food additive
    alpha-amylase
    dough
    bread
    quality
    Opis:
    Celem podjętych badań było określenie wpływu dodatku alfa-amylazy do mąki żytniej na właściwości ciasta i jakość pieczywa. Materiał badawczy stanowiły trzy próbki mąki żytniej typu 720 o niskiej i bardzo niskiej aktywności enzymów amylolitycznych oraz alfaamylaza, którą dodawano w ilości 15, 20 i 40 g·(100 kg mąki)-1. Aktywność amylolityczną próbek mąki wyjściowej i z dodatkiem alfaamylazy oceniano na podstawie badań amylograficznych i liczby opadania. Przeprowadzono badania reologiczne ciasta za pomocą mixolabu. Wykonano wypieki laboratoryjne. Stwierdzono, że dodatek alfa-amylazy do mąki żytniej powodował obniżenie wysokich wartości parametrów amylograficznych mąki: maksymalnej lepkości i temperatury końcowej kleikowania. Ciasto z mąki żytniej o większym dodatku alfa-amylazy cechowało się mniejszym oporem w punktach wykresu uzyskanego za pomocą mixolabu charakteryzującym właściwości skrobi: kleikowanie (opór ciasta w punkcie C3), podatność na działanie enzymów amylolitycznych (opór ciasta w punkcie C4) i retrogradację (opór ciasta w punkcie C5). Stwierdzono, że wraz ze zmniejszeniem oporu ciasta w punkcie C2, w którym rozpoczyna się kleikowanie skrobi i w punkcie C3 wykresu oraz tempa kleikowania skrobi (wskaźnik β) zwiększała się objętość chleba (współczynniki korelacji odpowiednio r = –0,670; r = –0,726 i r = –0,651). Jakość chleba pod wpływem dodatku alfa-amylazy ulegała poprawie – wzrastała objętość chleba i kwasowość miękiszu a zmniejszała się jego twardość.
    The aim of this study was to determine the effect of alpha-amylase addition on the properties of dough and on the quality of rye bread. The experimental material was three samples of rye flour type 720 with low and very low amylolytic activity. Alpha-amylase was added in the amounts of 15, 20 and 40 g (100 kg flour)-1. Amylolytic activity of tested rye flour without and with alpha-amylase addition was assessed by the amylograph properties and falling number method. The rheological properties of dough were tested by mixolab. The laboratory test baking was performed. Alpha-amylase addition to rye flour reduced high values of amylograph properties: maximum vis-cosity and final temperature of gelatinisation. Rye dough obtained from flour with higher alpha-amylase addition was characterised by lower mixolab torque in points which characterise starch gelatinisation (torque in point C3), enzymatic activity (torque in point C4) and retrogradation (torque in point C5). The rye dough with lower torque in point C2 in which gelatinization starts and in point C3 and lower slope β was characterised by higher bread volume (correlation coefficients: r = –0,670; r = –0,726; r = –0,651 respectively). Better quality of bread obtained from rye flour with alpha-amylase addition was observed – the bread volume and titrable acidity increased, and crumb hardness decreased.
    Źródło:
    Acta Agrophysica; 2014, 21, 2
    1234-4125
    Pojawia się w:
    Acta Agrophysica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zmiany chemiczne w procesie psucia się pieczywa natury mikrobiologicznej
    Khimicheskie prevrashhenija v periode porchi khlebnykh izdelijj svjazanykh s mikrobiologicheskimi processami
    Chemical changes in bread with microbial spoilage
    Autorzy:
    Bojankiewicz, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/877793.pdf
    Data publikacji:
    1963
    Wydawca:
    Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
    Tematy:
    pieczywo
    psucie sie zywnosci
    pieczywo pszenne
    enzymy amylolityczne
    Bacillus subtilis
    alfa-amylazy
    dekstryny
    zmiany chemiczne
    bread
    food spoilage
    wheat bread
    amylolytic enzyme
    alpha-amylase
    dextrin
    chemical change
    Źródło:
    Roczniki Państwowego Zakładu Higieny; 1963, 14, 3
    0035-7715
    Pojawia się w:
    Roczniki Państwowego Zakładu Higieny
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zbozowe bialkowe inhibitory enzymow hydrolitycznych i ich znaczenie. Czesc I. Bialkowe inhibitory alfa-amylaz
    Autorzy:
    Piasecka-Kwiatkowska, D
    Warchalewski, J.R.
    Powiązania:
    https://bibliotekanauki.pl/articles/828813.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Technologów Żywności
    Tematy:
    inhibitory enzymow
    alfa-amylazy
    enzymy hydrolityczne
    zboza
    enzymy amylolityczne
    ziarno
    bialka roslinne
    zywienie czlowieka
    enzyme inhibitor
    alpha-amylase
    hydrolytic enzyme
    cereal
    amylolytic enzyme
    seed
    vegetable protein
    human nutrition
    Opis:
    W ziarnie zbóż występują w znacznych ilościach białkowe substancje o charakterze inhibitorów enzymów hydrolitycznych. Pomimo, iż występowanie tych substancji w ziarnie zbóż stwierdzono już w latach trzydziestych, to do tej pory nie udało się dokładnie ich poznać. W pracy tej scharakteryzowano inhibitory enzymów amylolitycznych występujące w ziarnie pszenicy, żyta i pszenżyta oraz przedstawiono współczesne poglądy na temat ich znaczenia.
    Cereal seeds contain a lot of protein substances which are inhibitors of hydrolytic enzymes. Although, they were discovered in the early 1930's till today remain a number of question to be answer. In this paper protein nature alpha-amylase inhibitors found in grain of wheat, rye and triticale were discussed on the grounds of the present-day knowledge.
    Źródło:
    Żywność Nauka Technologia Jakość; 2000, 07, 2; 110-119
    1425-6959
    Pojawia się w:
    Żywność Nauka Technologia Jakość
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Some examples of the use of electrophoretic protein analysis in taxonomic investigations of leguminous plants
    Autorzy:
    Przybylska, J
    Powiązania:
    https://bibliotekanauki.pl/articles/2048196.pdf
    Data publikacji:
    1995
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Pisum
    genetic resource
    isoenzyme
    plant genetics
    electrophoresis
    leguminous plant
    dehydrogenase
    taxonomy
    albumin
    Lupinus
    globulin
    Phaseolus
    peroxidase
    Leguminosae
    legumin fraction
    phaseolin
    Papilionoideae
    amylase
    seed protein
    taxonomic investigation
    transaminase
    protein
    aminopeptidase
    phenotype
    esterase
    Opis:
    The article reviews the work of the author and coworkers on the use of electrophoretic protein analysis in taxonomie studies and classification of genetic resources of some leguminous plants: the genus Pisum, the Old-World Lupinus species and the cultivated Phaseolus species. On the example of the genus Pisum, the object of many years' investigations, the usefulness of electrophoretic analysis of different protein types - seed globulins, seed albumins and enzyme systems - is critically discussed. It has been concluded that variation in seed albumins, proteins often ignored in comparative analyses, should be more widely considered in taxonomie investigations. The conclusion was supported by the results obtained for Lupinus and Phaseolus species.
    Źródło:
    Journal of Applied Genetics; 1995, 36, 3; 255-271
    1234-1983
    Pojawia się w:
    Journal of Applied Genetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optymalizacja parametrow hydrolizy enzymatycznej skrobi ziemniaczanej polaczonej z procesem ekstruzji
    Optimization of the enzymatic hydrolysis of potato starch combined with extrusion
    Autorzy:
    Krzyzaniak, W
    Jankowski, T
    Grajek, W
    Powiązania:
    https://bibliotekanauki.pl/articles/827859.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Technologów Żywności
    Tematy:
    skrobia ziemniaczana
    hydroliza enzymatyczna
    ekstruzja
    alfa-amylazy
    preparaty enzymatyczne
    preparat Termamyl S
    preparat Ban 480 L
    potato starch
    enzymatic hydrolysis
    extrusion
    alpha-amylase
    enzymatic preparation
    Termamyl S preparation
    Ban 480 L preparation
    Opis:
    Celem pracy była optymalizacja parametrów hydrolizy enzymatycznej skrobi ziemniaczanej połączonej z procesem ekstruzji. Do hydrolizy użyto następujących preparatów enzymatycznych: α-amylazy bakteryjnej Termamyl S, Ban 480L oraz α-amylazy grzybowej Fungamyl 800L. Początkowo wykonano doświadczenia mające na celu dobranie parametrów pracy ekstrudera zapewniających dostateczne skleikowanie skrobi. Regulowano temperaturę w poszczególnych sekcjach ekstrudera oraz obroty elementów roboczych. Stwierdzono, że dopiero temperatura powyżej 100°C zapewniała dostateczne skleikowanie skrobi. Sprawdzono, czy w zastosowanych warunkach ekstruzji enzymy zachowują swoją hydrolityczną aktywność. Stwierdzono wzrost wielkości współczynnika DE wraz ze wzrostem dawki enzymu dodawanego do skrobi. Do dalszych ekstruzji wybrano enzymy o największej termoodporności czyli Termamyl S oraz Ban 480 L. Określono wpływ szybkości obrotów ślimaków, w zakresie 40–60 obr./min, oraz wilgotności początkowej skrobi, od 32 do 40%, na równoważnik glukozowy (DE) i ciśnienie osmotyczne badanych preparatów. Określono także skład chemiczny tych preparatów. Badania wykazały, że zastosowane warunki ekstruzji były wystarczające do częściowej hydrolizy skrobi ziemniaczanej i do uzyskania produktów o niskim stopniu scukrzenia.
    The objective of this paper was to optimize the process of simultaneous gelatinisation and liquefaction of potato starch with α-amylases in a co-rotating twin-screw extruder. Bacterial α-amylases, such as Termamyl S and Ban 480L, and fungal α-amylase Fungamyl 800L, were used. The first step was to determine the process parameters owing to which the gelatinisation of starch was possible. The temperature of the barrel sections and the screw speed of the extruder varied, and they were controlled, while the moisture content of starch was constant, and equal to 35% (w/w). It was stated that only a temperature higher than 100°C could produce a satisfactory gelatinisation of starch. It was also verified whether or not enzymes maintained their hydrolytic activity under the extrusion conditions. It was stated an increase in the dextrose equivalent (DE) ensuing parallel to the increasing doses of enzymes added to starch. For continuing extrusions, two enzymes: Termamyl S and Ban 480 L were selected since they showed the highest thermal resistance. The impact of the extruder’s screw speed (40–60 rpm) and of the initial moisture content in starch (32–40%) on DE and on the osmotic pressure of products under investigations was investigated. Additionally, chemical composition of these preparations was determined. The research performed showed that the conditions applied to carry out extrusion processes were sufficient for a partial hydrolysis of potato starch to occur products and to obtain products with a low DEvalue.
    Źródło:
    Żywność Nauka Technologia Jakość; 2005, 12, 1; 48-62
    1425-6959
    Pojawia się w:
    Żywność Nauka Technologia Jakość
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zbozowe, bialkowe inhibitory enzymow hydrolitycznych i ich znaczenie. Czesc II: Bialkowe inhibitory proteinaz
    Autorzy:
    Piasecka-Kwiatkowska, D
    Warchalewski, J.R.
    Powiązania:
    https://bibliotekanauki.pl/articles/826620.pdf
    Data publikacji:
    2000
    Wydawca:
    Polskie Towarzystwo Technologów Żywności
    Tematy:
    inhibitory enzymow
    alfa-amylazy
    enzymy hydrolityczne
    zboza
    enzymy amylolityczne
    ziarno
    bialka roslinne
    proteinazy
    inhibitory proteinaz
    zywienie czlowieka
    enzyme inhibitor
    alpha-amylase
    hydrolytic enzyme
    cereal
    amylolytic enzyme
    grain
    vegetable protein
    proteinase
    proteinase inhibitor
    human nutrition
    Opis:
    W pracy przedstawiono przegląd danych literaturowych na temat zbożowych, białkowych inhibitorów enzymów proteolitycznych. Szczególną uwagę zwrócono na ich właściwości, formy wielorakie, a także ich znaczenie biochemiczne, fizjologiczne i żywieniowe.
    The present state of knowledge on cereal proteinacious inhibitors of proteolytic enzymes is reviewed. Particular attention was given on their properties, multiple forms as well as significance from nutritional, physiological and biochemical point of view.
    Źródło:
    Żywność Nauka Technologia Jakość; 2000, 07, 3; 33-38
    1425-6959
    Pojawia się w:
    Żywność Nauka Technologia Jakość
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The influence of varying level of wheat proteinaceous inhibitors in wheat-based artificial feed on development parameters of selected stored product insects
    Autorzy:
    Warchalewski, J R
    Gralik, J.
    Winiecki, Z.
    Nawrot, J.
    Piasecka-Kwiatkowska, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/65768.pdf
    Data publikacji:
    2001
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    wheat starch
    varying level
    weevil
    plant protection
    wheat grain
    flour moth
    Sitophilus granarius
    insect
    Tribolium confusum
    feed
    insect foraging
    development parameter
    Anagasta kuehniella
    feeding intensity
    alpha-amylase inhibitor
    artificial wheat-based feed
    wheat
    larval development
    flour beetle
    artificial feed
    granary weevil
    Opis:
    The artificially made kernels from ground wheat grain, commercial wheat starch and wheat proteinaceous α-amylase inhibitors in different proportions were used as feed for adults of the granary weevil (Sitophilus granarius L.). In the case of larvae of the confused flour beetle (Tribolium confusum Duv.) and the Mediterranean flour moth (Anagasta kuehniella Zell.) the friable feed mixture were used. The survival of S. granarius adults has not been correlated with the soluble proteins extracted from wheat and amylolytic activity located in this protein fraction. On the other hand the weight of dust (the index of feeding intensity) produced during feeding has depended on the presence of α-amylase and trypsin inhibitors in wheat-based feed. A. kuehniella larvae have not developed at all on feed consisted of 50% wheat starch and 50% of crude a-amylase inhibitors from wheat. The same feed has caused 15.1 days of extension in development time of T. confusum larvae. lt attests to specific native enzymatic apparatus existing in alimentary canals of three damaging grain species which can overcome some obstacles even if extremely highly active insect α-amylase inhibitors were present in feed. However, the sufficient nutrient should be available in feed compounds. Nevertheless, some reduction of insects population can be expected.
    Sztuczne ziarna zrobione z handlowej skrobii pszennej i białkowych inhibitorów α-amylaz wymieszanych w różnych proporcjach oraz czyste ziarno pszenicy stanowiły pokarm wołka zbożowego (Sitophilus granaris L.). Dla larw trojszyka ulca (Trbolium confusum Duv.) i mklika mącznego (Anagasta kuchniella Zell.) te same produkty podawano w postaci sypkiej. Procent przeżywalności chrząszczy wołka zbożowego nie zależał od zawartości białka rozpuszczalnego ani od aktywności amylolitycznej pokarmu. Z drugiej strony ilość pyłu wytworzonego w czasie żerowania (wskaźnik intensywności żerowania) zależał od obecności inhibitorów α-amylazy i trypsyny. Larwy mklika mącznego nie kończyły rozwoju na pokarmie zawierającym 50% skrobii pszennej i 50 inhibitorów α-amylazy. Ten sam pokarm powodował przedłużenie rozwoju larw trojszyka ulca o 15,1 dnia. To świadczy o istnieniu wrodzonego, specyficznego aparatu enzymatycznego w przewodzie pokarmowym testowanych owadów, który może pokonać nawet ekstremalnie wysoką aktywność inhibitorów α-amylazy obecnych w pokarmie. Efektem działania inhibitorów może być tylko redukcja liczebności populacji.
    Źródło:
    Journal of Plant Protection Research; 2001, 41, 3
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena chemiczna i biochemiczna maki wybranych odmian pszenicy ozimej
    Autorzy:
    Czubaszek, A
    Subda, H
    Kowalska, M
    Korczak, B
    Zmijewski, M
    Karolini-Skaradzinska, Z
    Powiązania:
    https://bibliotekanauki.pl/articles/827286.pdf
    Data publikacji:
    2001
    Wydawca:
    Polskie Towarzystwo Technologów Żywności
    Tematy:
    analiza zywnosci
    enzymy proteolityczne
    alfa-amylazy
    pszenica ozima
    zawartosc skrobi
    maka pszenna
    zawartosc bialka
    pentozany
    bialko ogolne
    bialko rozpuszczalne
    odmiany roslin
    food analysis
    proteolytic enzyme
    alpha-amylase
    winter wheat
    starch content
    wheat flour
    protein content
    pentosan
    total protein
    soluble protein
    plant cultivar
    Opis:
    Materiał badawczy stanowiło 11 odmian pszenicy ozimej (Begra, Panda, Olcha, Almari, Gama, Wilga, Emika, Kobra, Roma, Jawa, Juma), ze Stacji Oceny Odmian w Tarnowie Śląskim, pochodzących ze zbioru w latach 1994-1996. Wykazano zmienność zawartości białka ogółem, białka rozpuszczalnego w soli sodowej siarczanu dodecylu (SDS), gluteniny wysokocząsteczkowej oraz białka nierozpuszczalnego. Stwierdzono, że mąka badanych odmian różniła się aktywnością enzymów proteolitycznych i a-amylazy, mierzonej liczbą opadania. Wykazano skorelowanie liczby opadania z zawartością białka ogółem i zawartością pentozanów rozpuszczalnych. Pentozany rozpuszczalne w większym stopniu różnicowały odmiany pszenicy niż pentozany nierozpuszczalne i zawartość pentozanów ogółem. Odmiany pszenicy różniły się zawartością skrobi w mące. Zawartość skrobi była istotnie skorelowana z zawartością białka ogółem i białka nierozpuszczalnego.
    he test material were 11 cultivars of winter wheat harvested in the years 1994-1996 (Begra, Panda, Olcha, Almari, Gama, Wilga, Emika, Kobra, Roma, Jawa, Juma), coming from the Strain Test Station at Tarnów Śląski. In the cultivars tested there was found a variability in the contents of total protein, SDS - soluble and insoluble protein, as well as of high-molecular glutenin. Their flour appeared to differ in the activity of proteolytic enzymes and alpha-amylase measured by the falling number. The falling number was proved to have been correlated with the contents of total protein and soluble pentosans. The wheat cultivars were more differentiated by soluble pentosans than by the insoluble and total ones. Different, too, was the content of starch in them, the latter having been significantly correlated with total and insoluble protein contents.
    Źródło:
    Żywność Nauka Technologia Jakość; 2001, 08, 1; 76-84
    1425-6959
    Pojawia się w:
    Żywność Nauka Technologia Jakość
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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