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Tytuł:
Selection of frost-tolerant cell lines from cell cultures of Solanum tuberosum L.
Autorzy:
Anjum, Muhammad Akbar
Powiązania:
https://bibliotekanauki.pl/articles/2198823.pdf
Data publikacji:
2001-06-21
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
potato
Solanum
cell culture
proline
hydoxyproline resistance
frost tolerance
Opis:
Fourteen hydroxyproline-resistant cell lines were selected by plating 7 days old cell suspensions of Solanum tuberosum L. cvs. Desiree and Maris Piper on a cell plating medium containing 5 or 10 mM hydroxyproline (hyp). Cell suspensions were either plated directly on selective media or after mutagenic treatment with gamma rays at a dose of 20 Gy or after freezing to –6°C. The frequency of resistant colonies varied from 0.15 to 0.35 x 10-6. Almost all the selected lines possessed increased levels of frost tolerance as compared to their non-selected controls except one indicating that hyp resistance and frost tolerance are not necessarily linked.
Źródło:
Plant Breeding and Seed Science; 2001, 45, 1; 3-10
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fractal morphology of Beta vulgaris L. cell suspension culture permeabilized with Triton X-100
Autorzy:
Arenas-Ocampo, M.
Alamilla-Beltran, L.
Vanegas-Espinoza, P.E.
Camacho-Diaz, B.H.
Campos-Mendiola, R.
Gutierrez-Lopez, G.
Jimenez-Aparicio, A.
Powiązania:
https://bibliotekanauki.pl/articles/25018.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
morphology
Beta vulgaris
cell suspension culture
Triton X-100
morphometry
cell culture
chemical agent
Opis:
n this work, morphology of Beta vulgaris L. cells permeabilized with 0.7 mM of Triton X-100® was evaluated using digital image processing and concepts of fractal dimension (perimeter- area relations). Important morphometric changes were found when the contact-time with chemical agent was increased.The size of cells decreased, the cells lost the roundness and their shape was more sinuous; this behaviour was a result of a probable shrinkage caused by the excess of exposure with the permeabili- zation agent. Morphology of B. vulgaris cells after permeabili- zation, exhibited a fractal nature since the slope of the ratio of the logarithm of the perimeter vs logarithm of the area was higher than unit. Fractal geometry of the cell morphology was affected as a re- sult of the exposure to Triton X-100®. Those changes can be attri- buted to the loss of turgor and structure of the cell wall.
Źródło:
International Agrophysics; 2012, 26, 1
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Trastuzumab Efficacy Quantified by Fluorine-19 Magnetic Resonance Imaging
Autorzy:
Bartusik-Aebisher, Dorota
Aebisher, David
Czmil, Mrs Anna
Mazur, Damian
Powiązania:
https://bibliotekanauki.pl/articles/895489.pdf
Data publikacji:
2020-06-29
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
trastuzumab
magnetic resonance imaging
breast cancer cells
three-dimensional cell culture
Trastuzumab conjugates
Opis:
The purpose of this study was to conjugate Trastuzumab with fluorine-bearing PAMAM dendrimer to compare activities in three-dimensional (3D) cultured breast cancer cells with parent Trastuzumab. An in vitro study was performed to determine cellular responses to fluorinated Trastuzumab conjugates by Magnetic Resonance Imaging (MRI). Breast cancer cells were cultured in 3D geometry. Proton (1H) MRI and Fluorine-19 (19F) MRI were used for visualization of cellular locations within a Hollow Fiber Bioreactor (HFBR) device and to monitor the cellular response to treatment. The results of this study confirm that cell growth is significantly decreased following treatment with Trastuzumab conjugates. The use of fluorinated Trastuzumab conjugates decreases breast cancer cell growth in 3D cultures and allows for tracking of drug delivery to cancer cells via 19F.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2020, 77, 3; 495-503
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Układ typu ciecz/ciecz jako alternatywna metoda hodowli przestrzennej komórek adherentnych
Liquid/liquid culture system as an alternative method of adherent cells 3-D culture
Autorzy:
Brzezińska, M.
Grabowska, I.
Dąbkowska, K.
Pilarek, M.
Powiązania:
https://bibliotekanauki.pl/articles/2071710.pdf
Data publikacji:
2012
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
układ hodowlany ciecz/ciecz
hodowla komórkowa
kule zarodkowe
mysie zarodkowe komórki macierzyste
liquid/liquid culture system
animal cell culture
cell spheres
Opis:
Porównano wzrost mysich zarodkowych komórek macierzystych w postaci kul zarodkowych w hodowli standardowej (stała powierzchnia pokryta żelatyną) oraz w układzie ciecz/ciecz (perfluorodekalina/pożywka). Wykazano, że hodowla kul zarodkowych w układzie dwóch niemieszających się faz ciekłych pozwala znacznie wydłużyć żywotność agregatów mysich komórek macierzystych oraz prawdopodobnie utrzymać je w stanie niezróżnicowanym.
The growth of mouse embryonic stem cell spheres cultured in the typical (on solid surface) and in liquid/liquid systems (perfluorodecalin/medium) has been compared. It has been showed that the liquid/liquid culture system enables one to extend the viability of cultured cells and probably maintains undifferentiated state of the mouse embryonic stem cell spheres.
Źródło:
Inżynieria i Aparatura Chemiczna; 2012, 4; 101-102
0368-0827
Pojawia się w:
Inżynieria i Aparatura Chemiczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Endothelial cells on pet vascular prostheses impregnated with polyester-based copolymers and coated with cell-adhesive protein assemblies
Autorzy:
Chlupac, J.
Filova, E.
Riedel, T.
Brynda, E.
Pamuła, E.
Lisa, V.
Bacakova, L.
Powiązania:
https://bibliotekanauki.pl/articles/284406.pdf
Data publikacji:
2008
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
vascular prostheses
polyethylene terephtalate
poly(glycolide-L-lactide)
poly(glycolide-L-lactide-(ε)caprolactone)
extracellular matrix
surface modification
collagen
laminin
fibronectin
fibrin
endothelial cells
static cell culture
Opis:
Arterial bypass surgery with synthetic vascular prostheses achieves poor patency rates compared to autogenous natural materials, and this is a challenge for tissue engineering research concerning small caliber vascular grafts. Modifications of the prosthetic surface followed by endothelial cell seeding may reduce thrombogenicity and intimal hyperplasia. Planar polyethylene terephthalate (PET) vascular prosthetic samples were impregnated with the copolymer poly(glycolide-L-lactide) (PGL) or with the terpolymer poly(glycolide-L-lactide-(e)caprolactone) (PGLCap) in order to lower the permeability of the knitted fabrics and ensure a less adhesive background. Subsequent modification with adhesive protein assemblies composed of collagen type I (Co) in conjunction with laminin (LM), fibronectin (FN) or fibrin (Fb) gel was performed to enhance cell adhesion. Bovine pulmonary artery endothelial cells (EC) of the CPAE line were seeded on to the coatings and subjected to static tissue culture conditions for 7 days. Impregnation of the PET prostheses decreased the initial adhesion and proliferation of the EC. After coating with the protein assemblies, the impregnated PET provided better substrates for cell culture than the protein-coated PET, on which the EC population started decreasing after 4 days of culture. The cells proliferated better on the CoFN, CoFb and CoFbFN coatings than on the Co and CoLM coatings. Impregnation type and adhesive matrix protein deposition may play an important role in successful endothelialization, healing and clinical performance of vascular grafts.
Źródło:
Engineering of Biomaterials; 2008, 11, no. 81-84; 108-111
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improvement of silymarin content in cell cultures of Silybum marianum by copper sulphate elicitor
Autorzy:
Elsharnouby, M.E.
Hassan, F.A.S.
Powiązania:
https://bibliotekanauki.pl/articles/11893796.pdf
Data publikacji:
2018
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
plant cultivation
milk thistle
Silybum marianum
herbal plant
medicinal plant
cell culture
silymarin
copper sulphate
Opis:
Silybum marianum L. (Milk thistle) extracts are the main source of silymarin that is a mixture of various flavonolignan (silybin (silibinin), silydianin and silychristin). Silymarin of milk thistle has a hepatoprotective activity for liver cirrhosis and chronic inflammatory. Silybum marianum regeneration from hypocotyl explants and evaluation of their callogenesis, growth and total flavolignan (silymarin) upon copper sulphate (as abiotic elicitor) elicitation was targeted. Copper sulphate (CuSO4) was applied in concentrations of 0, 3, 5, 7 and 9 µM to elicit the silymarin production in cultures. The elicitation periods used in this study were 2, 4, 7, 14 and 28 days. Half-strength MS medium recorded better results relative to full-strength MS one and seed incubation in the darkness at room temperature resulted in rapid germination and reached to the gar lid after 10 days. Callus fresh and dry weights as well as growth index were gradually increased with increasing the copper sulphate concentration till 5 µM while decreased thereafter at any elicitation period. With the increase of the elicitation period, the increase of the previous parameters was observed. Flavonolignan (silymarin) was positively correlated with CuSO4 levels since all levels of copper sulphate significantly enhanced its content in relative to the control. Additionally, more silymarin was accumulated after 4 or 7 days and the accumulation significantly decreased when the elicitation period reached 14 days more. The highest silymarin (flavolignan) content (11.79 and 11.67 mg g–1 DW) was obtained when 5 or 7 µM copper sulphate levels were combined with 4 days elicitation period, being about five-fold of the control.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2018, 17, 2; 105-114
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Właściwości i kliniczne możliwości zastosowania ludzkich komórek nabłonka owodni (HAEC)
Properties and clinical application of human amniotic epithelial cells (HAEC)
Autorzy:
Gawryluk, Arkadiusz
Noszczyk, Bartłomiej
Powiązania:
https://bibliotekanauki.pl/articles/1030397.pdf
Data publikacji:
2015
Wydawca:
Medical Communications
Tematy:
amniotic epithelial cells
amniotic membrane
biological dressing
cell culture
tissue engineering
hodowle komórkowe
inżynieria tkankowa
komórki nabłonka owodni
opatrunki biologiczne
owodnia
Opis:
In the contemporary medicine, undifferentiated progenitor cells of various origin and various degree of plasticity have become highly promising. Their most abundant, renewable and uncontroversial sources are placental tissues and umbilical blood. The only epithelial cells in this group come from the amnion which is used as a whole as an allogenic biological dressing. They have a range of unusual properties, such as the relative lack of histocompatibility antigens, plasticity (enabling their differentiation into a number of epithelial and mesenchymal cells) and the lack of neoplastic capacity. Amniotic epithelial cells are the only epithelial cells of the placenta. It is believed that they retain their progenitor (pluripotent) properties even in term pregnancies. This probably results from the fact that they omit the differentiation that accompanies gastrulation. Such features are typical of all placental cells which differ from amniotic epithelial cells only in their non-epithelial origin. In culture conditions, amniotic epithelial cells are characterized by a considerable plasticity: they can be stimulated to differentiate into adipocytes, chondrocytes, osteocytes, myocytes, cardiomyocytes, neurocytes, pancreatic cells and hepatocytes. To date, however, the attempts to direct their development towards the epidermis have not been successful. Obtaining multilayer epidermis in amniotic epithelial culture would be of considerable importance for tissue engineering of biological dressings. Amniotic membranes have been used for this purpose for many years, but because of their complex structure and metabolic requirements, they do not heal but dry up when applied to the wound. Some reports, however, indicate that the epithelium isolated from the amnion could be able to heal thus being suitable for allogenic grafts.
Współczesna medycyna coraz większe nadzieje pokłada w niezróżnicowanych komórkach progenitorowych różnego pochodzenia i o różnym stopniu plastyczności. Ich najbardziej zasobnym, odnawialnym i niekontrowersyjnym źródłem wydają się tkanki łożyska i krew pępowinowa. Jedyne w tej grupie komórki nabłonkowe pochodzą z owodni, wykorzystywanej często w całości jako allogeniczny opatrunek biologiczny. Mają one szereg niezwykłych cech, takich jak względny brak ekspresji antygenów zgodności tkankowej, plastyczność (umożliwiająca różnicowanie w cały szereg komórek nabłonkowych i mezenchymalnych) oraz brak zdolności do nowotworzenia. Komórki nabłonka owodni są jedynymi nabłonkowymi komórkami łożyska. Uważa się, że nawet w donoszonej ciąży zachowują właściwości progenitorowe (pluripotencjalne). Wynika to prawdopodobnie z faktu, iż pomijają różnicowanie towarzyszące gastrulacji. Cechy te przejawiają zresztą wszystkie komórki łożyska, różniące się od komórek nabłonka owodni jedynie nienabłonkowym pochodzeniem. W hodowli komórki nabłonka owodni charakteryzują się dużą plastycznością: ulegają stymulacji do różnicowania w kierunku adypocytów, chondrocytów, osteocytów, miocytów, kardiomiocytów, neurocytów, komórek trzustki i hepatocytów. Dotychczas nie udało się jednak skierować ich rozwoju w kierunku naskórka. Uzyskanie nabłonka wielowarstwowego w hodowli komórek nabłonka owodni miałoby ogromne znaczenie dla inżynierii tkankowej opatrunków biologicznych. Błony owodniowe wykorzystywane są w tym celu od wielu lat, jednak wskutek złożonej struktury i wymagań metabolicznych nie ulegają wgajaniu – wysychają po położeniu na powierzchni rany. Niektóre badania wskazują natomiast, że nabłonek izolowany z owodni mógłby się wgajać, nadawałby się zatem do allogenicznych przeszczepów.
Źródło:
Current Gynecologic Oncology; 2015, 13, 2; 123-135
2451-0750
Pojawia się w:
Current Gynecologic Oncology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cryptic rearrangements of chromosome 12 in testicular germ cell tumors with or without the specific i[12p] marker
Autorzy:
Grygalewicz, B
Pienkowska-Grela, B.
Woroniecka, R.
Powiązania:
https://bibliotekanauki.pl/articles/2043149.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
aberration
in situ
isochromosome
cytogenetic analysis
microdissection
testicular germ cell tumour
karyotype
cell culture
nonseminoma
fluorescence
hybridization
seminoma
chromosome 12
DNA
Źródło:
Journal of Applied Genetics; 2000, 41, 2; 123-131
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An integrated gas removing system for microfluidic application. Design and evaluation
Autorzy:
Hofman, I. M.
Ziółkowska, K.
Dybko, A.
Brzózka, Z.
Powiązania:
https://bibliotekanauki.pl/articles/115931.pdf
Data publikacji:
2013
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
gas removing system
debubbler
debubbling system
lab-on-a-chip
microfluidics
long-term cell culture
cell spheroids
HT-29 cells
Opis:
Microfluidic systems are used in a wide range of applications, including medical diagnostics, cell engineering and bioanalytics. In this work we focused on “Lab-on-a-chip” microsystems for cell cultivation. A troublesome problem of gas bubbles entering microdevices causing signal interferences and cells damage was emphasized. A novel, integrated debubbler in the form of cylindrical traps covered with thin PDMS membrane was designed and manufactured. Demonstrated debubbler was successfully applied in a long-lasting culture of HT-29 cell aggregates.
Źródło:
Challenges of Modern Technology; 2013, 4, 3; 10-15
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biological and biochemical characteristics of Spodoptera exigua nuclear polyhedrosis virus [SeMNPV] [Baculoviridae] stored for a long time at different formulations
Autorzy:
Jakubowska, A.
Ziemnicka, J.
Powiązania:
https://bibliotekanauki.pl/articles/66562.pdf
Data publikacji:
2003
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
insect population
Baculoviridae
commercial product
baculovirus
Spodoptera exigua
storage stability
cell culture
nuclear polyhedrosis virus
Źródło:
Journal of Plant Protection Research; 2003, 43, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Microfluidic devices — application in anticancer studies
Autorzy:
Jędrych, E.
Chudy, M.
Dybko, A.
Brzózka, Z.
Powiązania:
https://bibliotekanauki.pl/articles/115929.pdf
Data publikacji:
2012
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
microfluidic system
PDMS
adherent cell culture
concentration gradient generator (CGG)
cytotoxicity tests
photodynamic therapy (PDT) procedures
Opis:
A rapidly growing pharmaceutical industry requires faster and more efficient ways to find and test new drugs. One of the new method for cell culture and examining the toxic effects of drugs is application of microfluidic systems. They provide new types of microenvironments and new methods for investigation of anticancer therapy. The use of microsystems is a solution that gives the opportunity to reduce not only cost and time, but also a number of tests on animals. In this paper we present designed and fabricated hybrid microfluidic systems which are applicable for cell culture, cell based cytotoxicity assays and photodynamic therapy procedures. Polydimethylsiloxane (PDMS) and sodium glass were used for fabrication of microdevices. The designed geometry of the microdevices includes cell culture microchambers and a concentration gradient generator (CGG). The CGG enables to obtain diff erent concentrations of tested drugs in a single step, which is a significant simplification of cytotoxicity assay procedure. In the designed microsystems three various cell lines (normal and carcinoma) were cultured and analyzed.
Źródło:
Challenges of Modern Technology; 2012, 3, 2; 3-5
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New microfluidic device for lactate dehydrogenase (LDH) activity analysis
Autorzy:
Jędrych, E.
Mazur, M.
Grabowska-Jadach, I.
Brzózka, Z.
Powiązania:
https://bibliotekanauki.pl/articles/115778.pdf
Data publikacji:
2012
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
lactate dehydrogenase (LDH) activity
microfluidic system
PDMS
adherent cell culture
cytotoxicity analysis
Opis:
In this paper, we present cytotoxicity analysis (determination of lactate dehydrogenase — LDH activity performed in a designed and fabricated microfl uidic system. This method allowed for analysis of a supernatant collected from A549 (human lung cancer) and HT-29 (human colon cancer epithelial) cells, which were incubated for 24 h with selected compounds. LDH is an intracellular enzyme present in tissues, which is released into the supernatant caused by membrane damage or cell lyses. In our tests, LDH-Cytotoxicity Assay Kit (BioVision) was used. The toxic eff ect of drugs was measured in the developed microsystem made of PDMS (poly(dimethylsiloxane)). Analytical reaction took place in the special designed microchannel geometry. Then, the LDH activity was measured at 490 nm using spectrophotometer. In subsequent experiments, appropriate conditions for measurements using a microfl uidic system were chosen. It was found that the selected reagent is sensitive to temperature changes and light exposure. Reaction time in the microsystem was determined by changes of fl ow rates of reagents. Afterwards, for the various reaction time, the toxic eff ect of 5-fl uorouracil, celecoxib and 1,4-dioxane was performed. The obtained results were compared with the results carried out in 96-well plates. Based on these results, it was noted that the enzymatic reaction time in the microsystem is shorter than in 96-well plate. Moreover, the advantage of using microsystem is also the small amount of reagents.
Źródło:
Challenges of Modern Technology; 2012, 3, 4; 3-8
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Photodynamic therapy procedures in the microfluidic system
Autorzy:
Jędrych, E.
Pawlicka, Z.
Chudy, M.
Dybko, A.
Brzózka, Z.
Powiązania:
https://bibliotekanauki.pl/articles/115635.pdf
Data publikacji:
2010
Wydawca:
Fundacja na Rzecz Młodych Naukowców
Tematy:
photodynamic therapy (PDT)
microfl uidic culture system
PDMS
adherent cell culture
concentration gradient generator (CGG)
5-aminolevulinic acid (ALA)
Opis:
Evaluation of the effi ciency of photodynamic therapy (PDT) in a hybrid microfl uidic culture system was studied. The geometry of the utilized microsystem for PDT procedures consists microchambers for cell culture and microchannels, which create a concentration gradient generator (CGG). 5-aminolevulinic acid (ALA) as a precursor of the photosensitizer was used. The geometry of the microchip allowed to test diff erent concentrations of ALA in a single assay. Evaluation of the effi ciency of photodynamic therapy was determined 24 hours after PDT procedure (irradiation with light which induced accumulated in carcinoma cells). The performed microsystem contained two independent micropatterns, that enables examination simultaneously various cell lines (carcinoma and normal) or various photosensitizers.
Źródło:
Challenges of Modern Technology; 2010, 1, 1; 30-33
2082-2863
2353-4419
Pojawia się w:
Challenges of Modern Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cardiac endothelial cells isolated from mouse heart - a novel model for radiobiology
Autorzy:
Jelonek, Karol
Walaszczyk, Anna
Gabryś, Dorota
Pietrowska, Monika
Kanthou, Chryso
Widłak, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1039895.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cardiac endothelial cells
radiation
cardiotoxicity
primary cell culture
stress response
Opis:
Cardiovascular disease is recognized as an important clinical problem in radiotherapy and radiation protection. However, only few radiobiological models relevant for assessment of cardiotoxic effects of ionizing radiation are available. Here we describe the isolation of mouse primary cardiac endothelial cells, a possible target for cardiotoxic effects of radiation. Cells isolated from hearts of juvenile mice were cultured and irradiated in vitro. In addition, cells isolated from hearts of locally irradiated adult animals (up to 6 days after irradiation) were tested. A dose-dependent formation of histone γH2A.X foci was observed after in vitro irradiation of cultured cells. However, such cells were resistant to radiation-induced apoptosis. Increased levels of actin stress fibres were observed in the cytoplasm of cardiac endothelial cells irradiated in vitro or isolated from irradiated animals. A high dose of 16 Gy did not increase permeability to Dextran in monolayers formed by endothelial cells. Up-regulated expression of Vcam1, Sele and Hsp70i genes was detected after irradiation in vitro and in cells isolated few days after irradiation in vivo. The increased level of actin stress fibres and enhanced expression of stress-response genes in irradiated endothelial cells are potentially involved in cardiotoxic effects of ionizing radiation.
Źródło:
Acta Biochimica Polonica; 2011, 58, 3; 397-404
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In situ-formed bacterial exopolysaccharide (EPS) as a potential carrier for anchorage-dependent cell cultures
Autorzy:
Komorowski, Piotr
Kołodziejczyk, Agnieszka
Makowski, Krzysztof
Kotarba, Sylwia
Walkowiak, Bogdan
Powiązania:
https://bibliotekanauki.pl/articles/1844871.pdf
Data publikacji:
2021
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
bacterial exopolysaccharides
dextran- -based “microcarriers”
scanning electron microscopy
atomic force microscopy
roughness parameters
three-dimensional cell culture
Opis:
The study involved the use of a bacterial strain isolated from environmental samples which produce the biopolymer in the form of pellets in the submerged culture. This material (bacterial exopolysaccharide) is produced by bacteria of the Komogateibacter xylinus which are prevalent in the environment. The aim of this study was to characterize bacterial exopolysaccharides and commercial dextran-based “microcarriers” in terms of their roughness and cell culture effects, including the morphology and viability of the human hybridoma vascular endothelial cell line EA.hy926. The pellets were characterized using scanning electron microscopy (SEM) and atomic for¬ce microscopy (AFM). The resulting structures were used for cell culture of adherent cells (anchorage¬-dependent cells). At the same time, the cultures with commercial, dextran-based “microcarriers” were carried out for comparative purposes. After com¬pletion of the cell culture (24 hours of culture), the cellulose and commercial “carriers” were analyzed using SEM and AFM. Finally, the obtained cell dens¬ities (fluorescence labelling) and their morphological characteristics (SEM) were compared. The obtained results strongly support the applicability of bacterial exopolysaccharide (EPS) in tissue engineering to build innovative 3D scaffolds for cell culture, the more so that it is technologically possible to produce EPS as spatially complex structure
Źródło:
Engineering of Biomaterials; 2021, 24, 159; 18-23
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 34

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