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    Wyświetlanie 1-9 z 9
    Tytuł:
    Isolation and sequence analysis of the complete VP2 gene of canine parvovirus from Chinese domestic pets and determination of the pathogenesis of these circulating strains in beagles
    Autorzy:
    Chen, M.R.
    Guo, X.Y.
    Wang, Z.Y.
    Jiang, Y.T.
    Yuan, W.F.
    Xin, T.
    Hou, S.H.
    Song, T.Q.
    Lin, W.D.
    Zhu, H.F.
    Jia, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087542.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    canine parvovirus
    molecular epidemiology
    phylogenetic analysis
    pathogenesis
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 2; 287-296
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    MGF360-12L of ASFV-SY18 is an immune-evasion protein that inhibits host type I IFN, NF-κB, and JAK/STAT pathways
    Autorzy:
    Chen, Q.
    Wang, X.X.
    Jiang, S.W.
    Gao, X.T.
    Huang, S.Y.
    Liang, Y.
    Jia, H.
    Zhu, H.F.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647496.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    African swine fever virus
    MGF360-12L
    type I IFN
    NF-κB
    JAK/STAT
    Opis:
    African swine fever virus (ASFV) causes feverous and hemorrhagic disease of domestic pigs and European wild boars with high mortality, yet no commercial vaccine is currently available. Several ASFV strains with natural deletion or gene-targeted knockout of multiple MGF360 and MGF505 genes are attenuated in vitro and in vivo, and can offer full protection against homologous challenge. However, the mechanisms underlying the protection are not fully understood. This study aims to investigate the effects of MGF360-12L of ASFV-SY18 on the cGAS-STING signaling pathway and explore the potential mechanisms. We identified that ASFV-SY18 MGF360-12L could inhibit cGAS-STING, TBK1, or IRF3-5D-stimulated IFN-β expression and ISRE activation. Specifically, MGF360-12L inhibits both the activation of PRD(III-I) in a dose-dependent manner, and suppresses the exogenous expression of TBK1 and IRF3-5D. MGF360-12L could block NF-κB activation induced by overexpression of cGAS-STING, TBK1, IKKβ. Downstream of the IFN-β signaling, MGF360-12L blocks the ISRE promoter activation by reducing total protein level of IRF9. Moreover, MGF360-12L protein can inhibit IFN-β-mediated antiviral effects. In conclusion, our findings suggest that MGF360-12L is a multifunctional immune-evasion protein that inhibits both the expression and effect of IFN-β, which could partially explain the attenuation of relevant gene-deleted ASFV strains, and shed light on the development of efficient ASFV live attenuated vaccines in the future.
    Źródło:
    Polish Journal of Veterinary Sciences; 2023, 26, 1; 119-130
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Use of fluorescence quantitative polymerase chain reaction (PCR) for the detection of Escherichia coli adhesion to pig intestinal epithelial cells
    Autorzy:
    Dai, C.H.
    Gan, L.N.
    Qin, W.U.
    Zi, C.
    Zhu, G.Q.
    Wu, S.L.
    Bao, W.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/31510.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    An efficient and accurate method to test Escherichia coli (E. coli) adhesion to intestinal epithelial cells will contribute to the study of bacterial pathogenesis and the function of genes that encode receptors related to adhesion. This study used the quantitative real-time polymerase chain reaction (qPCR) method. qPCR primers were designed from the PILIN gene of E. coli F18ab, F18ac, and K88ac, and the pig β-ACTIN gene. Total deoxyribonucleic acid (DNA) from E. coli and intestinal epithelial cells (IPEC-J2 cells) were used as templates for qPCR. The 2−ΔΔCt formula was used to calculate the relative number of bacteria in cultures of different areas. We found that the relative numbers of F18ab, F18ac, and K88ac that adhered to IPEC-J2 cells did not differ significantly in 6-, 12-, and 24-well culture plates. This finding indicated that there was no relationship between the relative adhesion number of E. coli and the area of cells, so the method of qPCR could accurately test the relative number of E. coli. This study provided a convenient and reliable testing method for experiments involving E. coli adhesion, and also provided innovative ideas for similar detection methods.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prediction of moisture content uniformity using hyperspectral imaging technology during the drying of maize kernel
    Autorzy:
    Huang, M.
    Zhao, W.
    Wang, Q.
    Zhang, M.
    Zhu, Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/24685.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Instytut Agrofizyki PAN
    Tematy:
    prediction
    moisture content
    uniformity
    hyperspectral imaging
    drying
    maize kernel
    Opis:
    Moisture content uniformity is one of critical parameters to evaluate the quality of dried products and the drying technique. The potential of the hyperspectral imaging technique for evaluating the moisture content uniformity of maize kernels during the drying process was investigated. Predicting models were established using the partial least squares regression method. Two methods, using the prediction value of moisture content to calculate the uniformity (indirect) and predicting the moisture content uniformity directly, were investigated. Better prediction results were achieved using the direct method (with correlation coefficients RP = 0.848 and root-mean-square error of prediction RMSEP = 2.73) than the indirect method (RP = 0.521 and RMSEP = 10.96). The hyperspectral imaging technique showed significant potential in evaluating moisture content uniformity of maize kernels during the drying process.
    Źródło:
    International Agrophysics; 2015, 29, 1
    0236-8722
    Pojawia się w:
    International Agrophysics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Personal identification based on brain networks of EEG signals
    Autorzy:
    Kong, W.
    Jiang, B.
    Fan, Q.
    Zhu, L.
    Wei, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/329856.pdf
    Data publikacji:
    2018
    Wydawca:
    Uniwersytet Zielonogórski. Oficyna Wydawnicza
    Tematy:
    electroencephalogram signal
    personal identification
    brain network
    phase synchronization
    elektroencefalogram
    identyfikacja osobowa
    sieć mózgowa
    synchronizacja fazy
    Opis:
    Personal identification is particularly important in information security. There are numerous advantages of using electroencephalogram (EEG) signals for personal identification, such as uniqueness and anti-deceptiveness. Currently, many researchers focus on single-dataset personal identification, instead of the cross-dataset. In this paper, we propose a method for cross-dataset personal identification based on a brain network of EEG signals. First, brain functional networks are constructed from the phase synchronization values between EEG channels. Then, some attributes of the brain networks including the degree of a node, the clustering coefficient and global efficiency are computed to form a new feature vector. Lastly, we utilize linear discriminant analysis (LDA) to classify the extracted features for personal identification. The performance of the method is quantitatively evaluated on four datasets involving different cognitive tasks: (i) a four-class motor imagery task dataset in BCI Competition IV (2008), (ii) a two-class motor imagery dataset in the BNCI Horizon 2020 project, (iii) a neuromarketing dataset recorded by our laboratory, (iv) a fatigue driving dataset recorded by our laboratory. Empirical results of this paper show that the average identification accuracy of each data set was higher than 0.95 and the best one achieved was 0.99, indicating a promising application in personal identification.
    Źródło:
    International Journal of Applied Mathematics and Computer Science; 2018, 28, 4; 745-757
    1641-876X
    2083-8492
    Pojawia się w:
    International Journal of Applied Mathematics and Computer Science
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Differential expression of Toll - like receptor 4 signaling pathway genes in Escherichia coli F18 - resistant and - sensitive Meishan piglets
    Autorzy:
    Liu, Y.
    Gan, L.N.
    Qin, W.Y.
    Sun, S.Y.
    Zhu, G.Q.
    Wu, S.L.
    Bao, W.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/31946.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The Toll-like receptor 4 (TLR4) signaling pathway is an important inflammatory pathways associated with the progression of numerous diseases. The aim of the present study was to investigate the relationship between TLR4 signaling and resistance to Escherichia coli F18 in locally weaned Meishan piglets. Using a real-time PCR approach, expression profiles were determined for key TLR4 signaling pathway genes TLR4, MyD88, CD14, IFN-α, IL-1β and TNF-α in the spleen, thymus, lymph nodes, duodenum and jejunum of E. coli F18-resistant and -sensitive animals. TLR4 signaling pathway genes were expressed in all the immune organs and intestinal tissues, and the expression was generally higher in the spleen and lymph nodes. TLR4 transcription was higher in the spleen of sensitive piglets (p<0.05), but there was no significant difference in TLR4 mRNA levels in other tissues. Similarly, CD14 transcription was higher in lymph nodes of sensitive animals (p<0.05) but not in other tissues. IL-1β expression was higher in the spleen and in the duodenum of resistant piglets (p<0.05, p<0.01, respectively), and there were no significant differences in other tissues. There were also no significant differences in the expression of MyD88, TNF-α and IFN-α between sensitive and resistant piglets (p>0.05). These results further confirm the involvement of the TLR4 signaling pathway in resistance to E. coli F18 in Meishan weaned piglets. The resistance appeared to be mediated via downregulation of TLR4 and CD14, and upregulation of MyD88 that may promote the release of cytokines TNF-α, IL-1β, IFN-α and other inflammatory mediators which help to fight against E. coli F18 infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Stochastic hopf bifurcation of quasi-integrable hamiltonian systems with time-delayed feedback control
    Stochastyczna bifurkacja Hopfa w quasi-całkowalnych układach Hamiltonowskich sterowanych w pętli sprzężenia zwrotnego z opóźnieniem
    Autorzy:
    Liu, Z. H.
    Zhu, W. Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/280917.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
    Tematy:
    stochastyczna bifurkacja Hopfa
    quasi-całkowalny układ Hamiltonowski
    sprzężenie zwrotne z opóźnieniem
    stochastic Hopf bifurcation
    quasi-integrable Hamiltonian system
    stochastic averaging
    time-delayed feedback control
    Opis:
    The stochastic Hopf bifurcation of multi-degree-of-freedom (MDOF) quasi-integrable Hamiltonian systems with time-delayed feedback control subject to Gaussian white noise excitations is studied. First, the time-delayed feedback control forces are approximately expressed in terms of the system state variables without time delay, and the system is converted into anordinary quasi-integrable Hamiltonian system. The averaged It�o stochastic differential equations are derived by using the stochastic averaging method for quasi-integrable Hamiltonian systems. Then, an expression for the average bifurcation parameter of the averaged system is obtained approximately, and a criterion for determining the stochastic Hopf bifurcation caused by the time-delayed feedback control forces in the original system as the value of the average bifurcation parameter changing is proposed. An example is worked out in detail to illustrate the above criterion and its validity, and to show the effect of the time delay in the feedback control on the stochastic Hopf bifurcation of the system.
    W pracy zajęto się problemem stochastycznej bifurkacji Hopfa quasi-całkowalnych układów Hamiltonowskich o wielu stopniach swobody poddanych wymuszeniu białym szumem z układem sterowania opartym na pętli sprzężenia zwrotnego z opóźnie- niem. Najpierw znaleziono przybliżone wyrażenia na siły sterujące w funkcji zmiennych stanu układu bez opóźnienia, a następnie przetransformowano go postaci quasi- całkowalnej, Hamiltonowskiej. Wyprowadzono stochastyczne równania różniczkowe It^o za pomocą metody uśredniania układów quasi-całkowalnych. Znaleziono przybliżoną postać wyrażenia na parametr bifurkacyjny uśrednionego układu i zaproponowano kryterium stwierdzające obecność stochastycznej bifurkacji Hopfa wywołanej siłami sterującymi z opóźnieniem na podstawie wartości zmiany tego parametru. Opracowa- no szczegółowo przykład do ilustracji działania tego kryterium i zakresu jego stosowalności oraz do prezentacji wpływu opóźnienia w pętli sterownia na stochastyczną bifurkację Hopfa badanego układu.
    Źródło:
    Journal of Theoretical and Applied Mechanics; 2008, 46, 3; 531-550
    1429-2955
    Pojawia się w:
    Journal of Theoretical and Applied Mechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of polyacrylic acid on the surface properties of calcite and fluorite aiming at their selective flotation
    Autorzy:
    Zhang, C.
    Gao, Z.
    Hu, Y.
    Sun, W.
    Tang, H.
    Yin, Z.
    He, J.
    Guan, Q.
    Zhu, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/110577.pdf
    Data publikacji:
    2018
    Wydawca:
    Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
    Tematy:
    calcite
    fluorite
    selective flotation
    polyacrylic acid
    Opis:
    In this study, the polyacrylic acid (PAA) was studied as a selective depressant for calcite in the selective flotation of fluorite and calcite, and the implications of this process for the separation of fluorite ore were studied using micro-flotation tests, and the results were analyzed with Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) analyses. The flotation tests indicated that the preferential adsorption of PAA onto calcite rather than sodium oleate (NaOl) could selectively depress the flotation of calcite, allowing its separation from fluorite at pH 7. The zeta potential of calcite became more negative with the addition of PAA rather than with NaOl. However, the characteristic features of PAA adsorption were not observed for fluorite, suggesting that NaOl preferentially adsorbed on the surface of fluorite, or that PAA could be replaced by NaOl on the fluorite surface. FT-IR and XPS analysis were utilized to obtain a better understanding of the mechanism by which PAA was more strongly adsorbed on the calcite surface than NaOl. This was revealed to occur through chemical bonding between the carboxyl group of PAA and the hydroxyl groups of the Ca species on the calcite surface, modifying the structure of the adsorbed layer. A possible adsorption mechanism, along with a postulated adsorption mode for the surface interaction between PAA and calcite, is proposed.
    Źródło:
    Physicochemical Problems of Mineral Processing; 2018, 54, 3; 868-877
    1643-1049
    2084-4735
    Pojawia się w:
    Physicochemical Problems of Mineral Processing
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Fuzzy-PID control method for two-stage vibration isolation system
    Połączenie sterowania rozmytego z regulatorem PID w dwustopniowym układzie wibroizolacji
    Autorzy:
    Zhao, C-S.
    Zhu, S-J.
    He, Q-W.
    Powiązania:
    https://bibliotekanauki.pl/articles/280755.pdf
    Data publikacji:
    2007
    Wydawca:
    Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
    Tematy:
    two-stage vibration isolation system
    fuzzy-PID control
    simulation
    Opis:
    The arithmetic of PID control is simple and the stability of it is good. However, the dynamic characteristics of PID control are poor. The fuzzy logic control method is flexible and adaptive. It does not rely on a mathematical model of the system and can deal with the nonlinearity of parameters and uncertain problems. However, the stability of fuzzy logic control is poor. In this paper, fuzzy logic control combined with PID control is applied to a two-stage vibration isolation system to improve the vibration isolation effectiveness. Simulation results show that the method is effective.
    Arytmetyka regulatora PID jest prosta, a jego stabilność dobra. Jednakże charakterystyki dynamiczne PID już tak korzystne nie są. Z drugiej strony, metody sterowania oparte na logice rozmytej są elastyczne i cechuje je duża zdolność adaptacyjna. Nie polegają one na ścisłym modelu matematycznym danego układu i dobrze sobie radzą z nieliniowościami parametrów i słabo zdefiniowanymi zagadnieniami. Niestety stabilność sterowania z logiką rozmytą jest niedostateczna. W pracy zaprezentowano rodzaj kombinacji metody logiki rozmytej z regulatorem PID zastosowanej do dwustopniowego układu izolacji drgań. Przeprowadzone symulacje pokazały efektywność takiego podejścia.
    Źródło:
    Journal of Theoretical and Applied Mechanics; 2007, 45, 1; 171-177
    1429-2955
    Pojawia się w:
    Journal of Theoretical and Applied Mechanics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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