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Wyświetlanie 1-15 z 23
Tytuł:
Identification of a linear epitope in the capsid protein of goose astrovirus with monoclonal antibody
Autorzy:
Dai, G.
Huang, X.
Liu, Q.
Li, Y.
Zhang, L.
Han, K.
Yang, J.
Liu, Y.
Xue, F.
Zhao, D.
Powiązania:
https://bibliotekanauki.pl/articles/16647377.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
epitope
goose astrovirus
capsid protein
monoclonal antibody
Opis:
Goose astrovirus (GoAstV) is a novel avastrovirus that typically causes gosling gout and results in 2 to 20% mortality. GoAstV capsid protein is the sole structural protein, which is responsible for viral attachment, assembly, maturation as well as eliciting host antibodies. However, the epitopes within capsid protein have not been well studied. In this study, a monoclonal antibody, named 1D7, was generated against GoAstV capsid protein by hybridoma technology. Western blot results showed that this MAb could react with recombinant capsid protein expressed in E. coli. Also, it recognized the precursor of capsid protein, VP90 and VP70, in GoAstV-infected cells. Besides, excellent specificity of MAb 1D7 was further demonstrated in indirect immunofluorescence assay and immunohistochemical analysis. Epitope mapping results revealed that MAb 1D7 recognized the epitope 33QKVY 36 within Cap protein. Sequence alignment indicated that 33QKVY 36 is a conserved epitope among the isolates of goose astrovirus type 2 (GoAstV-2), suggesting the potential for its use in GoAstV-2 specific diagnostic assay. These findings may provide some insight into a function of the GoAstV capsid protein and further contribute to the development of diagnostic methods for GoAstV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 4; 579-587
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antibacterial spectrum of four compounds from yeasts in koumiss
Autorzy:
Chen, Y.J.
Du, C.G.
Guo, Y.Q.
Zhao, Y.F.
Aorigele, C.
Wang, C.J.
Simujide, H.
Aqima, W.
Zhang, X.Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087160.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Kluyveromyces marxianus
Saccharomyces cerevisiae
Escherichia coli
minimum inhibition concentration
minimum bactericidal concentration
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 2; 167-173
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Improvements in timber production of Chinese fir (Cunninghamia lanceolata) per unit forest area in China via tree breeding: Status and challenges
Autorzy:
Zhou, B.
Peng, D.
Zhao, Q.
Yangnan, S.
Yang, S.
Yang, F.
Qu, G.
Tang, W.
Ou, J.
Xiang, W.
Xiang, J.
Deng, X.
Powiązania:
https://bibliotekanauki.pl/articles/2077649.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Cunninghamia lanceolata
genetic improvement
timber volumes per unit forest area
wood properties
proposals
recommendations
Opis:
Chinese fir [Cunninghamia lanceolata (Lamb.) Hook] is a fast-growing tree species that is widely planted for timber production in southern China. Many high-quality breeding systems for Chinese fir have been developed using genetic methods. Here, we systematically review progress in increasing Chinese fir timber volumes per unit forest area in China, using genetic methods. We comprehensively assess the objec- tives, strategies, and primary accomplishments of the first to fourth generations of a Chinese fir breeding program. Furthermore, we propose a genetic improvement program and protocol for Chinese fir, and out- line several recommendations with respect to the main challenges associated with using genetic methods to increase timber yields. Finally, we present a series of proposals to increase Chinese fir timber production in China. This review offers insight into genetic improvements that have increased timber volumes of Chi- nese fir over four generations of breeding, and provides direction for future genetic improvements to wood properties.
Źródło:
Dendrobiology; 2020, 83; 43-51
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
n-sided polygonal hybrid finite elements involving element boundary integrals only for anisotropic thermal analysis
Autorzy:
Cao, R. F.
Zhao, X. J.
Lin, W. Q.
Wang, H.
Powiązania:
https://bibliotekanauki.pl/articles/38597074.pdf
Data publikacji:
2020
Wydawca:
Instytut Podstawowych Problemów Techniki PAN
Tematy:
hybrid finite element
polygon
non-conforming mesh
fundamental solution
anisotropic material
heat conduction
Opis:
As a combination of the traditional finite element method and boundary element method, the n-sided polygonal hybrid finite element method with fundamental solution kernels, named as HFS-FEM, is thoroughly studied in this work for two-dimensional heat conduction in fully anisotropic media. In this approach, the unknown temperature field within the polygon is represented by the linear combination of anisotropic fundamental solutions of problem to achieve the local satisfaction of the related governing equations, but not the specific boundary conditions and the continuity conditions across the element boundary. To tackle such a shortcoming, the frame temperature field is independently defined on the entire boundary of the polygonal element by means of the conventional one-dimensional shape function interpolation. Subsequently, by the hybrid functional with the assumed intra- and inter-element temperature fields, the stiffness equation can be obtained including the line integrals along the element boundary only, whose dimension is reduced by one compared to the domain integrals in the traditional finite elements. This means that the higher computing efficiency is expected. Moreover, any shaped polygonal elements can be constructed in a unified form with the same fundamental solution kernels, including convex and non-convex polygonal elements, to provide greater flexibility in meshing effort for complex geometries. Besides, the element boundary integrals endow the method higher versatility with a non-conforming mesh in the pre-processing stage of the analysis over the traditional FEM. No modification to the HFS-FEM formulation is needed for the non-conforming mesh and the element containing hanging nodes is treated normally as the one with more nodes. Finally, the accuracy, convergence, computing efficiency, stability of non-convex element, and straightforward treatment of non-conforming discretization are discussed for the present n-sided polygonal hybrid finite elements by a few applications in the context of anisotropic heat conduction.
Źródło:
Archives of Mechanics; 2020, 72, 2; 109-137
0373-2029
Pojawia się w:
Archives of Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ontogenetic stages of ceratopsian dinosaur Psittacosaurus in bone histology
Autorzy:
Zhao, Q.
Benton, M.J.
Hayashi, S.
Xu, X.
Powiązania:
https://bibliotekanauki.pl/articles/20498.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Opis:
The early ceratopsians Psittacosaurus and Protoceratops have provided important information on dinosaurian development because of abundant specimens of adults, subadults, juveniles, and even hatchlings. Here we present new data and methods for identifying key growth stages from bone histology. Previous studies on Psittacosaurus lujiatunensis from the Early Cretaceous Jehol Biota of China did not present in-depth analysis of growth patterns. Based on a histological study of 43 thin sections from 17 individuals of this species, we recognize four histological ontogenetic stages, i.e., hatchling, juvenile, sub-adult, and adult, but no fully-grown stage. We estimate life history and longevity from diaphyseal growth line counts and other features of histology. We show that P. lujiatunensis grew fast in early stages (hatchling, juvenile, and subadult), according to the density of vascular canals and the different type of bone tissue; the deposition of parallel fibred bone tissue in the outer cortex of the subadult stage indicates that growth rate was slowing down. We introduce a new graphical method to estimate the occurrence and volumes of vascular canals from thin sections more accurately than current two-dimensional approaches.
Źródło:
Acta Palaeontologica Polonica; 2019, 64, 2
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pharmacokinetics of the recombinant ovine interferon-tau in lambs
Autorzy:
Zhao, J.
Yu, H.Y.
Zhao, Y.
Li, S.Q.
Fu, X.L.
Zhou, W.
Xia, B.B.
Wang, M.L.
Chen, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087588.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bioavailability
ovine interferon-τ
cytopathic effect inhibition assay
pharmacokinetic study
Źródło:
Polish Journal of Veterinary Sciences; 2019, 1; 75-82
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A method for tracing exogenous DNA uptake in live spermatozoa and embryos
Autorzy:
Mu, Y.
Wang, J.
Liu, Z.
Zhao, Y.
Zhang, X.
Jiao, M.
Lv, J.
Hao, J.
Kong, Q.
Powiązania:
https://bibliotekanauki.pl/articles/2087751.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
spermatozoa
SMGT
exogenous DNA
IVF
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 193-202
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings
Autorzy:
Xu, T.
Huang, X.
Li, Y.
Liu, Q.
Liu, X.
Han, K.
Liu, Y.
Yang, J.
Zhao, D.
Bi, K.
Sun, W.
Powiązania:
https://bibliotekanauki.pl/articles/2087719.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
goose-derived
duck-derived
chicken-derived
Tembusu virus
pathogenicity
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 2; 389-396
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular cloning and expression analysis of CML27 in Brassica oleracea pollination process
Autorzy:
Lian, X.P.
Zeng, J.
Zhang, H.C.
Yang, X.H.
Zhao, L.
Zhu, L.Q.
Powiązania:
https://bibliotekanauki.pl/articles/19583.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2018, 60, 2
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimal expression and purification of sapelovirus A structural protein VP1, and its immunogenicity in mice
Autorzy:
Zhao, T.T.
Cui, L.
Chen, L.
Li, J.J.
Liang, Q.L.
Wu, P.J.
Yu, X.Q.
Zhang, Z.H.
Hua, X.G.
Powiązania:
https://bibliotekanauki.pl/articles/2087686.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
sapelovirus A
prokaryotic expression
purification
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 573-579
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The DC-side active filter with dual-buck full-bridge inverter for wind generators
Autorzy:
Chen, X-Q.
Zhao, S-W.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/141358.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
multi-pulse ac-dc converter
auto-transformer
harmonic suppression
DC-side active filter
dual-buck full-bridge inverter
Opis:
This paper proposes a new dc-side active filter for wind generators that combines 12-pulse polygon auto-transformer rectifier with dc-side current injection method and dual-buck full-bridge inverter having not the “shoot-through” problem in conventional bridge-type inverters, and therefore this system with the character low harmonic distortion and high reliability. The proposed dc-side active filter is realized by using dual-buck full bridge converter, which directly injects compensation current at dc-side of two six-pulse diode bridges rectifiers. Compared with the conventional three-phase active power filter at ac-side, the system with the dc-side active filter draws nearly sinusoidal current by shaping the diode bridges output current to be triangular without using the instantaneous reactive power compensation technology, only using simple hysteretic current control, even though under load variation and unbalanced voltage disturbances, and while an acceptable linear approximation to the accurate waveform of injection current is recommended. The performance of the system was simulated using MATLAB/Simulink, and the possibility of the dc-side active filter eliminating current harmonics was confirmed in steady and transient states. The simulation results indicate, the system has a total harmonic distortion of current reduced closely to 1%, and a high power factor on the wind generator side.
Źródło:
Archives of Electrical Engineering; 2018, 67, 2; 263--277
1427-4221
2300-2506
Pojawia się w:
Archives of Electrical Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
VvWRKY13 enhances ABA biosynthesis in Vitis vinifera
Autorzy:
Hao, J.
Ma, Q.
Hou, L.
Zhao, F.
Liu, X.
Powiązania:
https://bibliotekanauki.pl/articles/58583.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Botaniczne
Opis:
Abscisic acid (ABA) plays critical roles in plant growth and development as well as in plants’ responses to abiotic stresses. We previously isolated VvWRKY13, a novel transcription factor, from Vitis vinifera (grapevine), and here we present evidence that VvWRKY13 may regulate ABA biosynthesis in plants. When VvWRKY13 was ectopically expressed in Arabidopsis, the transgenic lines showed delayed seed germination, smaller stomatal aperture size, and several other phenotypic changes, indicating elevated ABA levels in these plants. Sequence analysis of several genes that are involved in grapevine ABA synthetic pathway identified WRKY-specific binding elements (W-box or W-like box) in the promoter regions. Indeed, transient overexpression of VvWRKY13 in grapevine leaves significantly increased the transcript levels of ABA synthetic pathway genes. Taken together, we conclude that VvWRKY13 may promote ABA production by activating genes in the ABA synthetic pathway.
Źródło:
Acta Societatis Botanicorum Poloniae; 2017, 86, 2
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Acer truncatum seedlings are more plastic than Quercus variabilis seedlings in response to different light regimes
Autorzy:
Guo, X.
Wang, R.-Q.
Wang, C.D.
Xu, F.
Zhao, S.
Guo, W.-H.
Powiązania:
https://bibliotekanauki.pl/articles/41339.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Acer truncatum
seedling
Quercus variabilis
Chinese cork oak
plant response
light regime
irradiance acclimation
chlorophyll fluorescence
morphology
photoinhibition
Opis:
In this study, we investigated responses of the mid-successional species Acer truncatum Bunge and the late-successional species Quercus variabilis Blume to three solar illumination conditions: (1) constant low light (CL), (2) constant high light (CH) and (3) low light first and high light afterwards (LH). The last treatment was to simulate a canopy opening. Both species exhibited increases in biomass, totally and in part, and decreases in leaf water content, specific leaf area and chlorophyll concentrations in LH treatment compared to CL treatment. For A. truncatum, exposure to high light condition (LH) increased crown area, and decreased root to shoot ratio, stem mass ratio and leaf perimeter. However, for Q. variabilis, LH treatment increased stem diameter at ground height, effective quantum yield, photochemical quenching and decreased maximum photosystem II quantum yield. The biomass allocation pattern did not change in Q. variabilis among three light conditions. With respect to newly developed leaves, no significant differences were found in leaf size of Q. variabilis between LH treatment and CH treatment while that of A. truncatum decreased in LH treatment. All chlorophyll fluorescence parameters in newly developed oak leaves in LH treatment increased compared to those of CH treatment while no difference was found for A. truncatum between LH and CH treatment. A. truncatum displayed a greater overall plasticity than Q. variabilis although the oak seedlings have a greater plasticity with respect to chlorophyll concentration and chlorophyll fluorescence parameters. A. truncatum should be a better candidate for vegetation recovery, especially in places with heterogeneous light conditions.
Źródło:
Dendrobiology; 2016, 76
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein
Autorzy:
Han, K.
Zhao, D.
Liu, Y.
Liu, Q.
Huang, X.
Yang, J.
Bi, K.
Xu, T.
Li, Y.
Powiązania:
https://bibliotekanauki.pl/articles/31495.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused massive economic losses to the duck industry in China. Envelope (E) protein of DTMUV is an important structural protein, which is able to induce protective immune response in target animals and can be used as specific serological diagnosis tool. In this study, a novel monoclonal antibody, designated mAb 3E9, was generated against DTMUV E protein. It is positive in indirect ELISA against both His-E protein and the purified whole viral antigen. Also, this mAb showed positive reaction with DTMUV in Western blot and indirect immunofluorescence assay, and the isotype was IgG1. End-point neutralizing assay performed in BHK-21 cells revealed that the neutralization titer of 3E9 against DTMUV JS804 strain reached 1:50. Furthermore, functional studies revealed that 3E9 blocks infection of DTMUV at a step on viral attachment. The anti-E mAbs produced in the present work may be valuable in developing an antigen-capture ELISA test for antigen detection or a competitive ELISA test for antibody detection or therapeutic medicine for DTMUV in poultry.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Heavy Metal Contamination of Surface Water and Groundwater in and Around Gejiu Tin Mine, Southwest China
Zanieczyszczenie wód powierzchniowych i podziemnych metalami ciężkimi w kopalni Gejiu Tin, południowo-zachodnie Chiny i jej okolicach
Autorzy:
Cheng, X.
Qi, W.
Danek, T.
Matysek, D.
Huang, Q.
Zhao, X.
Zhou, Z.
Fang, R.
Zou, L.
Xu, J.
Powiązania:
https://bibliotekanauki.pl/articles/317873.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Przeróbki Kopalin
Tematy:
metale ciężkie
zanieczyszczenie
kopalnia cyny Gejiu
wody powierzchniowe
wody kopalniane
heavy metals
contamination
Gejiu tin mine
surface water
mine water
Opis:
Heavy metal contamination due to mining activity is a global major concern because of its potential health risks to local inhabitants. The heavy metal contamination of surface water and ground water by mining activities in Gejiu tin-polymetallic mining area, Southwest China, was studied. Surface water and ground water were sampled and analyzed using AAS for Cr6+, Cd, As, Hg, Cu, Pb, Zn, Se, Fe and Mn. Analysis of HCO3–, Cl–, SO42–, F– and NO3– in water samples was also undertaken by ion chromatography. It was shown that none of water samples exceeded the guideline of Cr6+, Se and Hg, while the contamination degree of heavy metals was Mn > Fe > Cd > Zn > Pb > As > Cu, all of which were serious contamination except mild contamination for Cu. The ground waters were polluted much worse than surface water.
Zanieczyszczenie metalami ciężkimi spowodowane działalnością kopalń jest światowym problemem z powodu ryzyka utraty zdrowia przez okolicznych mieszkańców. Zbadano zanieczyszczenie wód powierzchniowych oraz wód podziemnych wywołane działalnością kopalni cyny w rejonie górniczym Gejiu, południowo-zachodnie Chiny. Pobrano próbki wody powierzchniowej oraz podziemnej i zanalizowano je ze względu na obecność Cd, As, Hg, Cu, Pb, Zn, Se, Fe oraz Mn. Analizy HCO3–, Cl–, SO42–, F– oraz NO3– w próbkach wody również zostały przeprowadzone za pomocą chromatografii jonowej. Ukazano, że żadna z badanych wód nie przekroczyła dopuszczalnych poziomów dla Cr6+, Se oraz Hg. Jednakże poziom zanieczyszczenia metali ciężkimi tj. Mn > Fe > Cd > Zn > Pb > As > Cu był wysoki, za wyjątkiem niewielkiego przekroczenia norm dla Cu. Wody podziemne były znacznie bardziej zanieczyszczone niż wody powierzchniowe.
Źródło:
Inżynieria Mineralna; 2016, R. 17, nr 1, 1; 93-98
1640-4920
Pojawia się w:
Inżynieria Mineralna
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 23

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