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Wyszukujesz frazę "Zhao, J. S." wg kryterium: Autor


Tytuł:
Improvements in timber production of Chinese fir (Cunninghamia lanceolata) per unit forest area in China via tree breeding: Status and challenges
Autorzy:
Zhou, B.
Peng, D.
Zhao, Q.
Yangnan, S.
Yang, S.
Yang, F.
Qu, G.
Tang, W.
Ou, J.
Xiang, W.
Xiang, J.
Deng, X.
Powiązania:
https://bibliotekanauki.pl/articles/2077649.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Cunninghamia lanceolata
genetic improvement
timber volumes per unit forest area
wood properties
proposals
recommendations
Opis:
Chinese fir [Cunninghamia lanceolata (Lamb.) Hook] is a fast-growing tree species that is widely planted for timber production in southern China. Many high-quality breeding systems for Chinese fir have been developed using genetic methods. Here, we systematically review progress in increasing Chinese fir timber volumes per unit forest area in China, using genetic methods. We comprehensively assess the objec- tives, strategies, and primary accomplishments of the first to fourth generations of a Chinese fir breeding program. Furthermore, we propose a genetic improvement program and protocol for Chinese fir, and out- line several recommendations with respect to the main challenges associated with using genetic methods to increase timber yields. Finally, we present a series of proposals to increase Chinese fir timber production in China. This review offers insight into genetic improvements that have increased timber volumes of Chi- nese fir over four generations of breeding, and provides direction for future genetic improvements to wood properties.
Źródło:
Dendrobiology; 2020, 83; 43-51
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ontogenetic stages of ceratopsian dinosaur Psittacosaurus in bone histology
Autorzy:
Zhao, Q.
Benton, M.J.
Hayashi, S.
Xu, X.
Powiązania:
https://bibliotekanauki.pl/articles/20498.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Instytut Paleobiologii PAN
Opis:
The early ceratopsians Psittacosaurus and Protoceratops have provided important information on dinosaurian development because of abundant specimens of adults, subadults, juveniles, and even hatchlings. Here we present new data and methods for identifying key growth stages from bone histology. Previous studies on Psittacosaurus lujiatunensis from the Early Cretaceous Jehol Biota of China did not present in-depth analysis of growth patterns. Based on a histological study of 43 thin sections from 17 individuals of this species, we recognize four histological ontogenetic stages, i.e., hatchling, juvenile, sub-adult, and adult, but no fully-grown stage. We estimate life history and longevity from diaphyseal growth line counts and other features of histology. We show that P. lujiatunensis grew fast in early stages (hatchling, juvenile, and subadult), according to the density of vascular canals and the different type of bone tissue; the deposition of parallel fibred bone tissue in the outer cortex of the subadult stage indicates that growth rate was slowing down. We introduce a new graphical method to estimate the occurrence and volumes of vascular canals from thin sections more accurately than current two-dimensional approaches.
Źródło:
Acta Palaeontologica Polonica; 2019, 64, 2
0567-7920
Pojawia się w:
Acta Palaeontologica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development and application of isothermal amplification methods for rapid detection of F4 fimbriae producing Escherichia coli
Autorzy:
Zhao, L.Y.
Niu, J.H.
Gao, X.L.
Liu, C.N.
Liu, S.M.
Jiang, N.
Lv, X.P.
Zheng, S.M.
Powiązania:
https://bibliotekanauki.pl/articles/2087340.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Escherichia coli
F4 fimbriae
isothermal amplification
rapid detection
clinical samples
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 1; 143-152
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pharmacokinetics of the recombinant ovine interferon-tau in lambs
Autorzy:
Zhao, J.
Yu, H.Y.
Zhao, Y.
Li, S.Q.
Fu, X.L.
Zhou, W.
Xia, B.B.
Wang, M.L.
Chen, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087588.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bioavailability
ovine interferon-τ
cytopathic effect inhibition assay
pharmacokinetic study
Źródło:
Polish Journal of Veterinary Sciences; 2019, 1; 75-82
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Dynamic stability of a stepped drillstring conveying drilling fluid
Autorzy:
Zhao, G.-H.
Tang, S.
Liang, Z.
Li, J.
Powiązania:
https://bibliotekanauki.pl/articles/280899.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
Tematy:
drill string
stepped fluid-conveying pipe
complex frequency
stability
FEM
Opis:
Taking into account differences between a drill pipe (DP) and a drill collar (DC), the drillstring in a vertical well is modeled as a stepped pipe conveying a drilling fluid downwards to the bottom inside the string and then upwards to the ground from the annulus. An analytical model that describes lateral vibration of the drillstring and involves the drillstring gravity, weight on bit (WOB), hydrodynamic force and damping force of the drilling fluid is established. By analysis of complex frequencies, the influences of WOB, borehole diameter, DP length, velocity and density of the drilling fluid on the stability of the system are discussed.
Źródło:
Journal of Theoretical and Applied Mechanics; 2017, 55, 4; 1409-1422
1429-2955
Pojawia się w:
Journal of Theoretical and Applied Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fuzzy-PID control method for two-stage vibration isolation system
Połączenie sterowania rozmytego z regulatorem PID w dwustopniowym układzie wibroizolacji
Autorzy:
Zhao, C-S.
Zhu, S-J.
He, Q-W.
Powiązania:
https://bibliotekanauki.pl/articles/280755.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
Tematy:
two-stage vibration isolation system
fuzzy-PID control
simulation
Opis:
The arithmetic of PID control is simple and the stability of it is good. However, the dynamic characteristics of PID control are poor. The fuzzy logic control method is flexible and adaptive. It does not rely on a mathematical model of the system and can deal with the nonlinearity of parameters and uncertain problems. However, the stability of fuzzy logic control is poor. In this paper, fuzzy logic control combined with PID control is applied to a two-stage vibration isolation system to improve the vibration isolation effectiveness. Simulation results show that the method is effective.
Arytmetyka regulatora PID jest prosta, a jego stabilność dobra. Jednakże charakterystyki dynamiczne PID już tak korzystne nie są. Z drugiej strony, metody sterowania oparte na logice rozmytej są elastyczne i cechuje je duża zdolność adaptacyjna. Nie polegają one na ścisłym modelu matematycznym danego układu i dobrze sobie radzą z nieliniowościami parametrów i słabo zdefiniowanymi zagadnieniami. Niestety stabilność sterowania z logiką rozmytą jest niedostateczna. W pracy zaprezentowano rodzaj kombinacji metody logiki rozmytej z regulatorem PID zastosowanej do dwustopniowego układu izolacji drgań. Przeprowadzone symulacje pokazały efektywność takiego podejścia.
Źródło:
Journal of Theoretical and Applied Mechanics; 2007, 45, 1; 171-177
1429-2955
Pojawia się w:
Journal of Theoretical and Applied Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nano-sized micelles formed by self-assembling of polylactide(ethylene glycol) block copolymers in aqueous solutions
Autorzy:
Yang, L.
Zhao, Z.
Wei, J.
Li, S.
Powiązania:
https://bibliotekanauki.pl/articles/284952.pdf
Data publikacji:
2007
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
kopolimery
copolymers
Źródło:
Engineering of Biomaterials; 2007, 10, no. 63-64; 16-18
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Functional traits related to environmental divergence in combination with phylogenetic relationship of Picea species
Autorzy:
Wang, M.
Wang, J.
Zhang, A.
Zhang, X.
Sun, S.
Zhao, C.
Powiązania:
https://bibliotekanauki.pl/articles/2077949.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
δ13C
P50
Picea
seeva
quantitative convergence index
Opis:
Background: Plants have adapted to fine-scale environmental heterogeneity through ecologically important traits, leading to new lineages. This suggests that differentiation of important traits has been beneficial to habitat partitioning among closely related species. However, the pattern of ecological divergences and differentiation of functional traits in combination with phylogenetic relationships have not been widely examined. Material and methods: To illustrate the pattern of ecological divergences of nine Picea species, spatial evolutionary and ecological vicariance analysis (seeva) was used to quantify and test the divergence between sister lineages. Five functional traits were measured in a common garden experiment. For all traits the quantitative convergence index (QVI) was determined. Furthermore, their relationships with divergent environmental variables were analyzed in the context of phylogenetic relationships. Results: Temperature variables (maximum temperature of warmest month and temperature annual range) split at basal nodes and precipitation variables (annual precipitation and precipitation of driest quarter) split at terminal nodes. Conservative traits were leaf mass per area (LMA) and net photosynthetic rate (Pn), which reflected selective retention when ancestors suffered cold environmental separation. Meanwhile, linear stomatal density (LSD), carbon-13 isotope ratio (δ13C) and water potential at 50% loss of hydraulic conductivity (P50) were convergent between species, but only P50 exhibited adaption to different precipitation conditions. The nine Picea species exhibit a distinct environmental divergence pattern. LMA and Pn were selectively retained when their ancestors were subjected to cold environmental separation. P50 was an important trait with respect to adaptation to precipitation differences. This research provided a new way of expounding the correlation between environment, functional traits and phylogeny, deepening our understanding of environmental divergence, trait differentiation and speciation.
Źródło:
Dendrobiology; 2018, 80; 131-142
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on radiation characteristics of plasma Yagi antenna based on AIS base station in ships’ routeing waters
Autorzy:
Sun, Y.
Chen, Y.
Kong, F.
Wei, Y.
Zhan, F.
Zhao, J. S.
Powiązania:
https://bibliotekanauki.pl/articles/117021.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
radio navigation
radiation characteristics
plasma Yagi antenna
ships routeing
AIS base station
AIS based shipping routes
ships routeing waters
Automatic Identification System (AIS)
Opis:
A Yagi plasma antenna model was established by HFSS according to the relationship between plasma dielectric constant and electron density. The patterns were simulated by changing plasma parameters and the number of director dipoles. Results show that when the passive vibrators were switched off, the antenna is omnidirectional antenna. The directionality increases with the increase of the number of passive dipole and the main lobe of which narrows down. Then the plasma Yagi antenna model is established by plasma tube, the gain changed by changing the number of passive dipoles, so the plasma Yagi antenna has a very good reconfigurability. Results prove that the feasibility of the plasma Yagi antenna can be used on AIS base station of Ships’ Routeing waters. It can promote the communication and capability of maritime supervision in Ships’ Routeing waters.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2020, 14, 1; 179-184
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research of Fluorinated Acrylate Copolymer on Flax Fabric
Badania wpływu emulsji kopolimeru akrylanowego zawierającej fluor na właściwości tkanin lnianych
Autorzy:
Sui, Z.
Yang, K.
Chen, J.
Zhao, X.
Gao, S.
Powiązania:
https://bibliotekanauki.pl/articles/234076.pdf
Data publikacji:
2017
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
Tematy:
fluorine-containing acrylate
core-shell polymerisation
linen fabric
finishing agent
hydrophobicity
lipophobicity
fluor
akrylan
polimeryzacja rdzeń-otoczka
tkanina lniana
hydrofobowość
lipofobowość
Opis:
A fluorine-containing acrylate copolymer emulsion was prepared in this study, which was applied to finishing linen fabric. Dodecafluloroheptry methacrylate (G04) was used as a modified monomer, butylacrylate (BA) and methylacrylate (MMA) as soft and hard monomers.respectively, and acrylic acid as a functional monomer. Structure and properties of the products were characterised and analyzed by FTIR, TEM, PSD and CA measurements, respectively. The water and air permeability as well as the breaking force of the fabric treated were also investigated. The results showed that the fluorine-containing acrylate copolymer emulsion obtained had a core-shell structure, and the microsphere was of nanoscale size. Because of the latex film on the fabric surface, it became smooth and flat. And the increase in G04 was beneficial to the mechanical properties, waterproofness and antifouling ability of the linen fabric. When the content of G04 were increased from 0% to 20%, the water in the linen sample and hexadecane contact angles to the copolymer film increased from 113.02° to 136.08° and from 65.1° to 87.6°,respectively. Furthermore the breaking force of the linen samples was enhanced from 648 N to 721N. However, the increasing content of G04 had negative effects on the flexural rigidity as well as water and air permeability of the fabric, which were essential requirements for the fabric’s application. However, the wash resistance was excellent and the wear-resistance of the finished linen fabric was better than the unfinished linen fabric.
W pracy przygotowano emulsję kopolimeru akrylanowego zawierającą fluor, którą następnie zastosowano do wykańczania tkanin lnianych. Jako modyfikowany monomer zastosowano metakrylan dodekafluoroheptan (G04), a jako miękkie i twarde monomery użyto akrylan butylu (BA) i akrylan metylu (MMA), a kwas akrylowy jako monomer funkcjonalny. Strukturę i właściwości produktów scharakteryzowano i przeanalizowano za pomocą pomiarów FTIR, TEM, PSD i CA. Przebadano również przepuszczalność wody i powietrza oraz siłę zrywania tkaniny poddanej obróbce. Wyniki wykazały, że otrzymana fluorowa emulsja kopolimeru akrylanowego miała strukturę rdzeń-otoczka, a mikrosfera miała rozmiar w nanometrach. Ze lateksowej powłoce tkanina stała się gładka i matowa. Stwierdzono, że wzrost G04 wpływa korzystnie na właściwości mechaniczne, wodoodporność i zdolność przeciwporostową tkaniny lnianej. Zwiększenie zawartości G04 spowodowało wzrost kąta zwilżania, a siła zrywania próbek zwiększyła się z 648 do 721 N. Jednak wzrastająca zawartość G04 miała negatywny wpływ na sztywność zginania, a także przepuszczalność wody i powietrza tkaniny, które były zasadniczymi cechami wymaganymi dla aplikacji tkaniny. Jednakże odporność na pranie i zużycie była lepsza w przypadku próbek powleczonych.
Źródło:
Fibres & Textiles in Eastern Europe; 2017, 6 (126); 83-88
1230-3666
2300-7354
Pojawia się w:
Fibres & Textiles in Eastern Europe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The implementation of the Balanced Critical Factor Index methodology in the strategy redevelopment process
Autorzy:
Rymaszewska, A.
Koskinen, J.
Takala, J.
Zhao, S.
Chen, Y.
Powiązania:
https://bibliotekanauki.pl/articles/407087.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Balanced Critical Factor Index
BCFI
decision-making
strategy
Opis:
Strategic planning and development is a critical yet often overlooked issue in company's operations. The planning of development as well as addressing the distribution of available resources should be done systematically rather than instinctively however, many companies still do not have any reliable method that would facilitate the decision making processes at their disposal. This article aims at presenting the innovative analytical method- Balanced Critical Factor Index (BCFI) for addressing the need for change in company's operational strategy according to changes in available technology and knowledge. By examining a wide array of indexes, the presented method is expected to suggest the directions of development. The presented method also addresses the challenges of the complex process of decision-making. Moreover, the empirical evidence gathered in the studied case company serves as a source of important feedback regarding the further improvement of the BCFI method.
Źródło:
Management and Production Engineering Review; 2013, 4, 1; 50-56
2080-8208
2082-1344
Pojawia się w:
Management and Production Engineering Review
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Thermal aging of hydrogenated nitrile rubber – loss of additives and its influence on elasticity maintenance
Starzenie termiczne uwodornionego kauczuku nitrylowego – ubytek dodatków i jego wpływ na zachowanie elastyczności
Autorzy:
Liu, X.
Zhao, J.
Yang, R.
Iervolino, R.
Barbera, S.
Powiązania:
https://bibliotekanauki.pl/articles/945800.pdf
Data publikacji:
2017
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Chemii Przemysłowej
Tematy:
hydrogenated nitrile rubber
thermal aging
additives
elasticity
uwodorniony kauczuk nitrylowy
starzenie termiczne
dodatki
elastyczność
Opis:
The thermal aging of hydrogenated nitrile rubber (HNBR) at elevated temperatures 125 and 150 °C was investigated. The changes of chemical structures, including functional groups and crosslinking density, and the changes of mechanical properties, including recovery from bending (RFB), tensile strength, elongation at break and Young's modulus, were determined. In addition, the loss of additives was analyzed by pyrolysis gas chromatography-mass spectrometry (Py-GC/MS). The aging procedure of HNBR exhibited two stages: loss of additives along with the deterioration of elasticity; obvious oxidation and extra crosslinking with further hardening and embrittlement. The relationship between the deterioration of elasticity and the loss of plasticizers was established. It was demonstrated that the failure of HNBR happened long before obvious oxidation and crosslinking of the rubber chains, and the loss of additives, especially the loss of plasticizers, was the main reason for the failure. Therefore, the elasticity recovery of aged HNBR immersed in the plasticizer (n-propyl palmitate as a model) was carried out. This treatment was demonstrated to be effective before obvious oxidation and crosslinking of HNBR occurred during the thermal aging.
Badano starzenie termiczne uwodornionego kauczuku nitrylowego (HNBR) w temperaturze 125 i 150 °C. Określono zmiany struktur chemicznych, w tym grup funkcyjnych i gęstości usieciowania oraz zmiany właściwości mechanicznych, w tym powrotu poodkształceniowego po zginaniu (RFB), wytrzymałości na rozciąganie, wydłużenia przy zerwaniu i modułu Younga. Ponadto metodą pirolitycznej chromatografii gazowej z detekcją mas (Py-GC/MS) analizowano straty dodatków obecnych w kauczuku. Procedura starzenia się HNBR wykazywała dwa etapy: ubytek dodatków wraz z pogorszeniem elastyczności; wyraźne utlenianie i dodatkowe sieciowanie związane z dalszym utwardzaniem i zwiększaniem kruchości. Określono zależność pomiędzy zmniejszaniem się elastyczności a ubytkiem dodatków zmiękczających. Wykazano, że pogorszenie właściwości HNBR następowało znacznie wcześniej niż utlenianie i sieciowanie oraz było spowodowane utratą dodatków, a zwłaszcza plastyfikatorów. W związku z tym przeprowadzono próbę odtworzenia elastyczności starzonych próbek HNBR przez zanurzenie w plastyfikatorze (palmitynian n-propylu jako model). Obróbka ta okazała się skuteczna, pod warunkiem przeprowadzenia jej zanim podczas starzenia termicznego nastąpiło utlenianie i sieciowanie HNBR.
Źródło:
Polimery; 2017, 62, 7-8; 588-598
0032-2725
Pojawia się w:
Polimery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
On the topological properties of the certain neural networks
Autorzy:
Liu, J.-B.
Zhao, J.
Wang, S.
Javaid, M.
Cao, J.
Powiązania:
https://bibliotekanauki.pl/articles/91804.pdf
Data publikacji:
2018
Wydawca:
Społeczna Akademia Nauk w Łodzi. Polskie Towarzystwo Sieci Neuronowych
Tematy:
neural network
topological indices
graph theory
Opis:
A topological index is a numeric quantity associated with a network or a graph that characterizes its whole structural properties. In [Javaid and Cao, Neural Computing and Applications, DOI 10.1007/s00521-017-2972-1], the various degree-based topological indices for the probabilistic neural networks are studied. We extend this study by considering the calculations of the other topological indices, and derive the analytical closed formulas for these new topological indices of the probabilistic neural network. Moreover, a comparative study using computer-based graphs has been carried out first time to clarify the nature of the computed topological descriptors for the probabilistic neural networks. Our results extend some known conclusions.
Źródło:
Journal of Artificial Intelligence and Soft Computing Research; 2018, 8, 4; 257-268
2083-2567
2449-6499
Pojawia się w:
Journal of Artificial Intelligence and Soft Computing Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ship recognition and tracking system for intelligent ship based on deep learning framework
Autorzy:
Liu, B.
Wang, S. Z.
Xie, Z. X.
Zhao, J. S.
Li, M. F.
Powiązania:
https://bibliotekanauki.pl/articles/117419.pdf
Data publikacji:
2019
Wydawca:
Uniwersytet Morski w Gdyni. Wydział Nawigacyjny
Tematy:
intelligent ship
deep learning framework
ship recognition system
ship tracking system
ship recognition and tracking system
intelligent navigation
autonomous ship
maritime safety
Opis:
Automatically recognizing and tracking dynamic targets on the sea is an important task for intelligent navigation, which is the prerequisite and foundation of the realization of autonomous ships. Nowadays, the radar is a typical perception system which is used to detect targets, but the radar echo cannot depict the target’s shape and appearance, which affects the decision-making ability of the ship collision avoidance. Therefore, visual perception system based on camera video is very useful for further supporting the autonomous ship navigational system. However, ship’s recognition and tracking has been a challenge task in the navigational application field due to the long distance detection and the ship itself motion. An effective and stable approach is required to resolve this problem. In this paper, a novel ship recognition and tracking system is proposed by using the deep learning framework. In this framework, the deep residual network and cross-layer jump connection policy are employed to extract the advanced ship features which help enhance the classification accuracy, thus improves the performance of the object recognition. Experimentally, the superiority of the proposed ship recognition and tracking system was confirmed by comparing it with state of-the-art algorithms on a large number of ship video datasets.
Źródło:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation; 2019, 13, 4; 699-705
2083-6473
2083-6481
Pojawia się w:
TransNav : International Journal on Marine Navigation and Safety of Sea Transportation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The impacts of BSMV on vegetative growth and water status in hulless barley (Hordeum vulgare var. nudum) in VIGS study
Autorzy:
Liang, J.
Chen, X.
Zhao, H.
Yu, S.
Long, H.
Deng, G.
Pan, Z.
Yu, M.
Powiązania:
https://bibliotekanauki.pl/articles/57548.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Hordeum vulgare var.nudum
hulled barley
barley
BSMV zob.barley stripe mosaic virus
barley stripe mosaic virus
vegetative growth
water status
VIGS zob.virus induced gene silencing method
virus induced gene silencing method
Opis:
Barley stripe mosaic virus (BSMV) is an established and extensively used virus-induced gene silencing (VIGS) vector for gene function analysis in monocots. However, the phenotypes generated by targeted gene silencing may be affected or masked by symptoms of BSMV infection. To better understand the potential effects of BSMV-VIGS in hulless barley (Hordeum vulgare var. nudum), the accumulation pattern of BSMV and its impacts on vegetative growth and water status were investigated. The results indicated that the vegetative growth of infected plants was significantly and continuously impacted by BSMV from 10 to 40 days post inoculation (dpi). When the accumulation of BSMV was extremely high (7 to 11 dpi), infected plants displayed twisted leaf tips with an increased water lose rate (WLR) and decreased water content (WC). Virus accumulation declined and stabilized after 25 dpi, at this stage, the WLR and WC were unaffected in the infected plants. The efficiency of VIGS was tested by the silencing of Phytoene desaturase (PDS). RT-qPCR indicated that BSMV-VIGS can be sustained with good efficiency for up to 40 dpi under an altered condition with lower temperature (22 ±1°C) and higher relative humidity (70 ±10%). It was concluded that 25 to 40 dpi was the appropriate time zone for drought-related gene analysis by BSMV-VIGS under such condition.
Źródło:
Acta Societatis Botanicorum Poloniae; 2015, 84, 1
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł

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