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    Wyświetlanie 1-51 z 51
    Tytuł:
    The occurrence of Toxoplasma gondii and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from East Poland with the use of PCR
    Autorzy:
    Sroka, J
    Szymanska, J.
    Wojcik-Fatla, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/51137.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    tick
    Ixodes ricinus
    Toxoplasma gondii
    Borrelia burgdorferi
    occurrence
    polymerase chain reaction
    genotyping
    B1 gene
    human population
    infection
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 313-319
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Knowledge, protection behaviours and seroprevalence of Lyme borreliosis in inhabitants of Lublin Province, eastern Poland – evaluation of a prophylaxis programme
    Autorzy:
    Zając, V.
    Sroka, J.
    Wójcik-Fatla, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/28763061.pdf
    Data publikacji:
    2023
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2023, 30, 3; 413-424
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Efficacy of the detection of Legionella in hot and cold water samples by culture and PCR. II. Examination of native samples from various sources
    Autorzy:
    Stojek, N.M.
    Wojcik-Fatla, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/50046.pdf
    Data publikacji:
    2012
    Wydawca:
    Instytut Medycyny Wsi
    Opis:
    A total of 123 water samples were examined in parallel by culture and semi-nested PCR for the presence of Legionella. They comprised: 35 samples of hot water distributed by the urban municipal water supply system (MWSS) taken in institutions, 45 samples of hot water distributed by urban MWSS taken in dwellings, 27 samples of cold water distributed by rural MWSS taken in dwellings, and 16 samples of cold well water taken in rural areas. The greatest frequency of the isolation of Legionella by culture (88.6%) was recorded in the samples of hot water from the urban institutions, having been greater compared to all other sources (p<0.001). The frequency of Legionella isolation from hot water in urban dwellings (28.9%) was significantly greater compared to the combined value (2.3%) for cold water from rural MWSS and wells (p<0.001). Strains belonging to Legionella pneumophila serogroups 2-14 predominated in the examined samples, while strains of L. pneumophila serogroup 1 and strains of Legionella spp. (other than L. pneumophila) were 3-fold less numerous. The rates of positive findings in the semi-nested PCR (stage 2) were greater than culture isolations in all kinds of samples, except for urban institutions. The correlation between the culture and PCR results was positive for samples of hot water from urban MWSS (p<0.01), but not for samples of cold water from rural MWSS and wells (p>0.5). A significant correlation was found between rates of PCRpositive results and numbers of Legionella pneumophila serogroups 2-14 strains, but not for other Legionella serogroups or species. In conclusion, our results support the opinion that though PCR cannot be a substitute for the isolation of Legionella by culture, it could be regarded as an useful complementary method.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2012, 19, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Efficacy of the detection of Legionella in hot and cold water samples by culture and PCR. I. Standardization of methods
    Autorzy:
    Wojcik-Fatla, A.
    Stojek, N.M.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/50884.pdf
    Data publikacji:
    2012
    Wydawca:
    Instytut Medycyny Wsi
    Opis:
    The aim of the present study was: – to compare methods for concentration and isolation of Legionella DNA from water; – to examine the efficacy of various modifications of PCR test (PCR, semi-nested PCR, and real-time PCR) for the detection of known numbers of Legionella pneumophila in water samples artificially contaminated with the strain of this bacterium and in randomly selected samples of environmental water, in parallel with examination by culture. It was found that filtration is much more effective than centrifugation for the concentration of DNA in water samples, and that the Qiamp DNA Mini-Kit is the most efficient for isolation of Legionella DNA from water. The semi-nested PCR and real-time PCR proved to be the most sensitive methods for detection of Legionella DNA in water samples. Both PCR modifications showed a high correlation with recovery of Legionella by culture (p<0.01), while no correlation occurred between the results of one-stage PCR and culture (p>0.1).
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2012, 19, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena wspolwystepowania Toxaplasma gondii i Borrelia burgdorferi w kleszczach Ixodes ricinus w oparciu o badania PCR
    Autorzy:
    Wojcik-Fatla, A.
    Sroka, J.
    Zajac, V.
    Cisiak, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/839306.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    pasozyty
    Toxoplasma gondii
    Borrelia burgdorferi
    wspolwystepowanie
    kleszcze
    Ixodes ricinus
    metody badan
    lancuchowa reakcja polimerazy
    Źródło:
    Annals of Parasitology; 2010, 56, 3; 207
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Risk of tick-borne diseases in various categories of employment among forestry workers in eastern Poland
    Autorzy:
    Cisak, E.
    Zajac, V.
    Wojcik-Fatla, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51019.pdf
    Data publikacji:
    2012
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2012, 19, 3
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Badania seroepidemiologiczne w kierunku Francisella tularensis wśród pracowników leśnictwa i rolników
    Seroepidemiological study on Francisella tularensis in forestry workers and farmers
    Autorzy:
    Cisak, E.
    Wojcik‑Fatla, A.
    Zajac, V.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/4445.pdf
    Data publikacji:
    2014
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Medycyna Ogólna i Nauki o Zdrowiu; 2014, 20(49), 4
    2083-4543
    Pojawia się w:
    Medycyna Ogólna i Nauki o Zdrowiu
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Repellents and acaricides as personal protection measures in the prevention of tick-borne diseases
    Autorzy:
    Cisak, E.
    Wojcik-Fatla, A.
    Zajac, V.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/50494.pdf
    Data publikacji:
    2012
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2012, 19, 4
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Hantavirus RNA not detected in Ixodes ricinus ticks
    Autorzy:
    Wojcik-Fatla, A.
    Zajac, V.
    Knap, J.P.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/49890.pdf
    Data publikacji:
    2011
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    hantavirus
    RNA
    Ixodes ricinus
    tick
    epidemiology
    polymerase chain reaction
    Polska
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2011, 18, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Hantavirus RNA was not detected in Dermacentor reticulatus ticks
    Autorzy:
    Wojcik-Fatla, A.
    Zajac, V.
    Knap, J.P.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51730.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Opis:
    A total of 190 Dermacentor reticulatus ticks (80 males, 110 females) collected on the territory of Ostrów Lubelski, Suchawa, Zalutyń and Kazimierz Dolny (Lublin Province, eastern Poland) were examined by reverse transcription PCR and nested PCR methods for the presence of hantavirus RNA. None of the examined Dermacentor reticulatus specimens showed the presence of the hantavirus-specific RNA in spite of using two pairs of primers and the clearly positive results obtained with the positive control. Thus, the hypothesis about the possible participation of ticks in the transmission of hantaviruses was not confirmed.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 3
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Seroepidemiological study of Lyme borreliosis among forestry workers in Southern Poland
    Autorzy:
    Buczek, A
    Rudek, A.
    Bartosik, K.
    Szymanska, J.
    Wojcik-Fatla, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/49580.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Lyme borreliosis
    borreliosis
    serology
    ELISA test
    forestry
    worker
    Polska
    forestry worker
    tick-borne disease
    diagnosis
    antibody
    treatment
    Borrelia burgdorferi
    tick
    immunoglobulin
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 257-261
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Coincidence of three pathogens [Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti] in Ixodes ricinus ticks in the Lublin macroregion
    Autorzy:
    Wojcik-Fatla, A
    Szymanska, J.
    Wdowiak, L.
    Buczek, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51180.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    tick
    Ixodes ricinus
    coincidence
    pathogen
    Borrelia burgdorferi
    Anaplasma phagocytophilum
    Babesia microti
    Lublin macroregion
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 151-158
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of fruit on the extracellular enzyme profiles of fungi
    Autorzy:
    Farian, E.
    Cholewa, G.
    Cholewa, A.
    Matczuk, M.
    Wójcik-Fatla, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/28762964.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 4; 562-567
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Fungal diversity of root vegetables and soil rhizosphere collected from organic and conventional farms in Eastern Poland
    Autorzy:
    Kłapeć, T.
    Cholewa, G.
    Cholewa, A.
    Dutkiewicz, J.
    Wójcik-Fatla, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081696.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    filamentous fungi
    vegetables
    rhizosphere soil
    organic and conventional farms
    Opis:
    Objective. Determination of the concentration and species composition of filamentous fungi in root vegetables (carrots and red beetroots), and the adjacent rhizosphere soil, collected on organic and conventional farms in Eastern Poland. Materials and method. During the period 2015–2016, a total number of 80 samples of vegetables and 40 samples of soil were examined. From each type of farm, 20 samples of vegetables and 20 samples of the adjacent soil were examined. In addition, the study included 20 samples of vegetables from organic farms and 20 samples of vegetables from conventional farms purchased on the markets in the city of Lublin in Eastern Poland. In order to determine the concentration and species composition of filamentous fungi, both in vegetables and soil, the method of plate dilutions on Malt Agar medium (Difco) with chloramphenicol was used. The study was conducted in two parallel repetitions. Inoculated media were incubated at the temperature of 30° C for 72 hours, then at room temperature for 72 hours. The species composition of fungal flora was determined using macroscopic and microscopic methods, with the help of keys and atlases. Results. It was found that the mean concentration of fungi was higher in vegetables and soil from conventional farms than in those from organic farms. In the case of carrots and soil from conventional farms, this concentration was 4.93 and 5.10 log10 CFU g-1, respectively, whereas from organic farms – respectively, 3.81 and 4.20 log10 CFU g-1. In the case of beetroots and soil from conventional farms, the mean concentrations were also higher compared to organic farms – 5.09 vs. 3.93 and 4.95 vs. 4.23 log10 CFU g-1. In the examined vegetables and rhizosphere soil, 61 species of filamentous fungi were found, of which 12 belonged to the genus Penicillium, 4 to the genus Fusarium, and 2 species each to the genera: Aspergillus, Cladosporium and Trichoderma. As many as 28 (45.9%) fungal species that occurred in vegetables and soil are regarded as pathogenic for humans.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 2; 374-381
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Profilaktyka chorób odkleszczowych
    Prevention of tick-borne diseases
    Autorzy:
    Dutkiewicz, J
    Cisak, E.
    Wójcik-Fatla, A.
    Zając, V.
    Sroka, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/179873.pdf
    Data publikacji:
    2014
    Wydawca:
    Centralny Instytut Ochrony Pracy
    Tematy:
    choroby odkleszczowe
    choroby zawodowe
    patogeny
    profilaktyka
    tick-borne diseases
    occupational
    pathogens
    prevention
    Opis:
    Chorobami odkleszczowymi nazywamy choroby zakaźne przenoszone za pośrednictwem drobnych pajęczaków, zwanych kleszczami (lxodida). W Polsce są one wywoływane przez co najmniej 5 gatunków (lub grup gatunków) mikroorganizmów (wirusów, bakterii, pierwotniaków). Spośród nich największe znaczenie ma krętek Borrelia burgdorferi wywołujący boreliozę z Lyme, który jest częstą przyczyną chorób zawodowych u leśników i rolników. Główne kierunki profilaktyki chorób odkleszczowych obejmują: szczepienia ochronne przeciwko kleszczowemu zapaleniu mózgu i opon mózgowo-rdzeniowych, unikanie spożywania surowego mleka, stosowanie odpowiedniej odzieży ochronnej podczas pracy w lesie lub na obrzeżach lasu, przegląd ciała i wzięcie natrysku po powrocie z pracy w lesie, szybkie i umiejętne usuwanie kleszczy przyssanych do ciała, stosowanie repelentów, edukację zdrowotną, opiekę medyczną nad zagrożonymi pracownikami leśnictwa i rolnictwa, działania prewencyjne ze strony pionu bhp lasów państwowych, redukcję liczebności kleszczy w środowisku naturalnym.
    Tick-borne diseases are infectious diseases transmitted by small arachnids called ticks (lxodida). In Poland, these diseases are caused by at least five species (or groups of species) of microorganisms (viruses, bacteria. protozoa). Of which, the most important is the spirochete Borrelia burgdorferi causing Lyme borreliosis, which is the frequent cause of occupational diseases in the forestry and agricultural workers. The main directions of the prevention of tick-borne diseases include: vaccination against tick-borne encephalitis, avoiding of the consumption of raw milk, use of the proper clothing during work in the forest, inspection of the body and taking shower after coming from forest. quick and proper removing of ticks adhered to body, use of repelltents, health education, providing medical care for endangered forestry and agricultural workers, preventive actions pursued by the management of the forest service, reduction of the tick number of natural environment.
    Źródło:
    Bezpieczeństwo Pracy : nauka i praktyka; 2014, 4; 21-23
    0137-7043
    Pojawia się w:
    Bezpieczeństwo Pracy : nauka i praktyka
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Risk of Lyme disease at various sites and workplaces of forestry workers in eastern Poland
    Autorzy:
    Cisak, E.
    Wojcik-Fatla, A.
    Zajac, V.
    Sroka, J.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/52094.pdf
    Data publikacji:
    2012
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2012, 19, 3
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optimization of flotation, DNA extraction and PCR methods for detection of Toxoplasma gondii oocysts in cat faeces
    Autorzy:
    Sroka, J.
    Karamon, J.
    Dutkiewicz, J.
    Wójcik-Fatla, A.
    Cencek, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081971.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    flotation
    Toxoplasma gondii
    faeces
    Real time PCR
    nested PCR
    cats
    oocysts detection
    Opis:
    Introduction and objective. The aim of the study was to compare the effectiveness of selected oocysts concentration methods, DNA extraction protocols and PCR assays targeting the B1 gene, for the development of procedures which would be effective and useful in laboratory practice for the detection of T. gondii in faecal samples from cats. Materials and method. In order to compare the influence of the flotation fluids on microscopy and PCR detection of T. gondii, saturated solutions of saccharose, MgSO4, ZnSO4 and NaNO3 were used. To determine the sensitivity of PCR tests used: Real time PCR (RT) and nested PCR, water samples spiked with T. gondii tachyzoites and oocysts were tested. DNA was extracted using DNeasy Blood and Tissue Kit (Qiagen) (K1). The same PCR tests were used to assess the efficacy of T. gondii DNA detection in samples of cat faeces spiked with oocysts, using DNA extraction by a K1 set and a K2 set (QIamp DNA Stool Mini Kit, (Qiagen). Results. The initial results showed that NaNO3 was most useful as a flotation fluid due to the lack negative effect on the oocysts and amplification efficacy in PCR. The level of detection for water samples (100 μl) was determined as 100 tachyzoites and 1–50 oocysts in RT, and 2–20 oocysts in nested PCR. The limit of detection (LD) for stool samples (250 mg) spiked with oocysts, where the K1 set was used, determined as 250 and 5 oocysts in RT and nested PCR, respectively. For samples extracted with the K2 set, LD in RT was determined as 1–50 oocysts (depending on the variant) and 50 oocysts in nested PCR. Conclusions. The most effective methods for detection of T. gondii in cat faeces seem to be centrifugal flotation with NaNO3, followed by DNA extraction with removing of inhibitors (K2 set) and Real Time PCR targeting B1 gene.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 680-685
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biological agents as occupational hazards - selected issues
    Autorzy:
    Dutkiewicz, J.
    Cisak, E.
    Sroka, J.
    Wojcik-Fatla, A.
    Zajac, V.
    Powiązania:
    https://bibliotekanauki.pl/articles/50867.pdf
    Data publikacji:
    2011
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    biological agent
    occupational hazard
    health hazard
    bioaerosol
    allergy
    human disease
    toxin
    virus
    bacteria
    fungi
    infectious disease
    zoonosis
    tick-borne disease
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2011, 18, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on Giardia duodenalis and Cryptosporidium spp. infection in veterinarians in Poland
    Autorzy:
    Wójcik-Fatla, A.
    Sroka, J.
    Zając, V.
    Zwoliński, J.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081928.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Giardia duodenalis
    Cryptosporidium spp.
    veterinarians
    Polska
    Opis:
    The main aim of the study was to determine the risk of two selected zoonosis infections caused by Giardia duodenalis and Cryptosporidium spp. among an occupational group of veterinarians in Poland. Two hundred and ninety-seven samples of stool were tested for the presence of Giardia cysts and Cryptosporidium oocysts using Direct Fluorescent Assay (DFA). There were no positive results for Cryptosporidium. The presence of Giardia cysts was found in two samples of faeces (0.67%). The risk with regard to the parasites Giardia duodenalis and Cryptosporidium spp. seems to be low among the group of veterinarians.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 732-733
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Missions of the Helicopter Emergency Medical Service in rural and urban areas in Poland - a comparative retrospective analysis
    Autorzy:
    Rzońca, P.
    Gałązkowski, R.
    Wójcik-Fatla, A.
    Panasiuk, L.
    Gotlib, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2083142.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Helicopter Emergency Medical Service
    rural and urban areas
    prehospital care
    Opis:
    Introduction. Mortality due to various causes, despite continuous efforts to improve the quality of medical services, is a serious problem for modern healthcare systems. Ischaemic heart disease and stroke are the cause of over 15 million deaths annually, and are therefore known as the world’s number one killer. Objective. The aim of this study is to characterise the missions and the most common reasons for dispatching Helicopter Emergency Medical Service (HEMS) crews, with special emphasis on the differences between urban and rural areas. Materials and method. The study was conducted using a retrospective analysis of HEMS missions, including flights to accidents and diseases carried out by HEMS crews in Poland from January 2014 – December 2018. The final analysis included 35,213 cases of HEMS missions. Results. The study group consisted mainly of male patients (66.40%), aged 50–64 (22.06%), mean age of the entire analysed group – 47.71 (SD: 25.96). The main reason for HEMS missions were strokes (21.63%). Analysis of patients’ clinical status revealed that the clinical status of patients treated in rural areas was more severe, which was indicated by the Glasgow Coma Scale – GCS (12.03 vs 12.35) and the Revised Trauma Scale – RTS (10.14 vs 10.60) scores. When assessed by the National Advisory Committee for Aeronautics (NACA) score, body injuries and fatal diseases were observed more often in patients in rural areas (NACA 7 6.12% vs 3.46%) (p<0.05). Conclusions. Multi-organ injuries, head traumas, sudden cardiac arrest, traumatic brain injuries, collapse and epilepsy, were more frequent reasons for HEMS missions in rural areas than in urban areas.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2019, 26, 2; 355-360
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Profilaktyka chorób odzwierzęcych innych niż kleszczowe
    Prevention of zoonotic diseases other than tick-borne
    Autorzy:
    Dutkiewicz, J
    Wójcik-Fatla, A.
    Zając, V.
    Sroka, J.
    Cisak, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/179719.pdf
    Data publikacji:
    2014
    Wydawca:
    Centralny Instytut Ochrony Pracy
    Tematy:
    choroby odzwierzęce
    patogeny
    drogi przenoszenia
    profilaktyka
    zoonoses
    occupational
    pathogens
    transmission routes
    prevention
    Opis:
    W Polsce występuje co najmniej 31 ważnych patogenów (wirusy, bakterie, grzyby, pierwotniaki, robaki), wywołujących zawodowe choroby odzwierzęce inne niż odkleszczowe. Spośród tych patogenów 9 przekazywanych jest głównie drogą powietrzną, 9 - drogą pokarmową i 13 - drogą kontaktu bezpośredniego przez skórę i/lub błony śluzowe. Najważniejsze kierunki profilaktyki chorób odzwierzęcych obejmują: utrzymywanie czystości w pomieszczeniach dla zwierząt i dokonywanie okresowych dezynfekcji, stosowanie odzieży ochronnej i sprzętu ochrony osobistej, profilaktykę medyczną, wybijanie zagrożonych stad, tępienie gryzoni, szczepienia ochronne ludzi i zwierząt, zachowanie szczególnej ostrożności przy odbieraniu porodów u przeżuwaczy, przestrzeganie zasad higieny przy spożywaniu pokarmów i wody i edukację zdrowotną.
    In Poland, there are at least 31 important pathogens (viruses, bacteria, fungi, protozoa, helminths) causing occupational zoonotic diseases other than tick-borne. Of that number, 9 are transmitted mainly by airborne route, 9 by alimentary route and 13 by direct contact through the skin and/or mucous membranes.The main directions using protective clothing and equipment, medical prophylaxis, eradicating endangered flocks, combating rodents, vaccinating humans and animals, taking precautions at parturitions of ruminants, maintaining hygiene at food and water consumption, and health education.
    Źródło:
    Bezpieczeństwo Pracy : nauka i praktyka; 2014, 6; 12-14
    0137-7043
    Pojawia się w:
    Bezpieczeństwo Pracy : nauka i praktyka
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of tick-borne encephalitis virus [TBEV] in samples of raw milk taken randomly from cows, goats and sheep in Eastern Poland
    Autorzy:
    Cisak, E
    Wojcik-Fatla, A.
    Zajac, V.
    Sroka, J.
    Buczek, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/50068.pdf
    Data publikacji:
    2010
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    tick-borne encephalitis virus
    prevalence
    raw milk
    milk
    milk sample
    cow
    goat
    sheep
    Polska
    epidemiology
    antibody
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2010, 17, 2; 283-286
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on tick-borne rickettsiae in eastern Poland. I. Prevalence in Dermacentor reticulatus (Acari: Amblyommidae)
    Autorzy:
    Wojcik-Fatla, A.
    Cisak, E.
    Zajac, V.
    Sroka, J.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/50634.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Rickettsia
    Rickettsia raoultii
    Polska
    prevalence
    Dermacentor reticulatus
    tick
    Acari
    Amblyommidae
    Opis:
    Rickettsia spp. transmitted by ticks are classified mostly in the Spotted Fever Group Rickettsiae (SFGR). Numerous species of this group have been identified in Eurasia as emerging pathogens, but still little is known about their occurrence, effects on human health, and co-incidence with other tick-borne pathogens. The aim of the presented study was to determine the prevalence of Rickettsia spp. in adult Dermacentor reticulatus (Acari: Amblyommidae) ticks collected in Lublin province of eastern Poland using the PCR method. The infection rate of D. reticulatus with Rickettsia spp. was 53.0%. All except one rickettsial isolates showed 100% homology with Rickettsia raoultii. A high prevalence of R. raoultii in Dermacentor reticulatus ticks from eastern Poland suggests that the SFGR species should be considered as potential causative agents of tick-borne diseases in this area.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Leptospirosis as a tick-borne disease? Detection of Leptospira spp. in Ixodes ricinus ticks in eastern Poland
    Autorzy:
    Wojcik-Fatla, A.
    Zajac, V.
    Cisak, E.
    Sroka, J.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51864.pdf
    Data publikacji:
    2012
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2012, 19, 4
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on tick-borne rickettsiae in eastern Poland. II. Serological response of occupationally exposed populations
    Autorzy:
    Zajac, V.
    Wojcik-Fatla, A.
    Cisak, E.
    Sroka, J.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51448.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    tick
    Rickettsia
    Polska
    serological response
    occupational exposure
    population
    serology
    ELISA test
    forestry worker
    agricultural worker
    Opis:
    A group of 150 persons living in the Lublin province of eastern Poland and occupationally exposed to tick bite were examined by the immunoenzymatic ELISA test for the presence of antibodies against tick-borne Spotted Fever Group (SFG) rickettsiae. The group consisted of 75 forestry workers employed in 3 forest inspectorates and 75 agricultural workers living in 2 villages. As a control group, 43 urban dwellers living in the city of Lublin and not occupationally exposed to tick bite were examined. Among 150 persons occupationally exposed to tick bite, the presence of antibodies against SFG rickettsiae was found in 54 (36.0% of the total). In the control group, the frequency of positive findings was only 4.7%, being significantly smaller compared to the exposed group (p=0.0001). Within the exposed group, the percentage of positive results in forestry workers (50.7%) was greater than in agricultural workers (21.3%); the difference was statistically significant (p=0.0002). Also within this group, the frequency of positive findings in males(46.5%) was significantly greater than in females (21.9%) (p=0.0029). In the exposed group, the positive results tended to increase with the age of the examined persons. However, a significant relationship between age and positive findings was found only in forestry workers (c2=14.207, p=0.00264), but not in agricultural workers and total exposed workers. The frequencies of positive results in forestry workers varied significantly depending on place of work (c2=11.271, p=0.00357). Similarly, the difference between the positive reactions in agricultural workers living in 2 villages proved to be significant (34.2% vs. 8.1%; p=0.0074). The obtained results indicate that people occupationally exposed to tick bite and living in the area of eastern Poland where over half of Dermacentor reticulatus ticks harbour SFG rickettsiae, are under significantly increased risk of infection with these rickettsiae.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The occurrence of Toxoplasma gondii infection in people and animals from rural environment of Lublin region - estimate of potential role of water as a source of infection
    Autorzy:
    Sroka, J
    Wojcik-Fatla, A.
    Szymanska, J.
    Dutkiewicz, J.
    Zajac, V.
    Zwolinski, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/49789.pdf
    Data publikacji:
    2010
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    parasite
    protozoan parasite
    Toxoplasma gondii
    occurrence
    people
    animal
    domestic animal
    rural environment
    Lublin region
    epidemiology
    serological examination
    farm inhabitant
    potable water
    polymerase chain reaction
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2010, 17, 1; 125-132
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Cognitive functions, lipid profile, and Apolipoprotein E gene polymorphism in postmenopausal women
    Autorzy:
    Bojar, I.
    Owoc, J.
    Wojcik-Fatla, A.
    Raszewski, G.
    Stanciak, J.
    Raczkiewicz, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/51620.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2015, 22, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of Toxoplasma gondii infection in cats in southwestern Poland
    Autorzy:
    Sroka, J.
    Karamon, J.
    Dutkiewicz, J.
    Wójcik-Fatla, A.
    Zając, V.
    Cencek, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081746.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    PCR
    seroprevalence
    Toxoplasma gondii
    IFAT
    cats
    oocysts
    coproscopy
    Opis:
    Objective. An assessment of the prevalence of Toxoplasma gondii infection in cats from southwestern Poland using serology, coproscopy and PCR methods. Materials and method. In total, 208 cats (139 females and 68 males), aged 0.5–12 years (mean=2.6) from 25 localities in southwestern Poland were examined by indirect immunofluorescence assay (IFAT) to estimate the T. gondii serological status. Faecal samples of 41 cats were examined for the presence of oocysts/DNA T. gondii by microscopy and Real-time/nested PCR. After flotation (with NaNO3), pellets from faecal samples were disrupted by 10 cycles of freezing (liquid nitrogen) and warming. DNA was extracted using QIamp DNA Stool Mini Kit (Qiagen), according to the manufacturer’s instruction. Results. The positive results in IFAT for anti-T. gondii IgG and IgM antibodies were found in 143 of 208 tested cats (68.8%). Among positive results, 14.5%, 34.1% and 51.4% were detected in titre ranges 128–512, 1,000–2,000 and ≥ 4,000, respectively. In 23.1% of cat sera anti- T. gondii IgM antibodies were found. The prevalence of anti-Toxoplasma antibodies was significantly greater in older cats (>1 year) (83.5%) than in younger cats (48.3%) (P<0.05), in females (74.1%) than in males (58.8%) (P<0.05), and in cats kept outdoors than indoors (69.7% vs. 16.7%) (P<0.01). Among the 41 faecal samples examined, the presence of structures resembling T. gondii oocysts was found in 2 samples (4.9%), and for one of these samples (2.4% of the total) the result was also confirmed by PCR. Conclusions. T. gondii infection in domestic cats is highly prevalent in southwestern Poland. Information on the prevalence of infection in cats can be useful for assessing T. gondii environmental contamination and the risk for public health.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 3; 1-5
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Preliminary study on the occurrence of Toxoplasma gondii in Ixodes ricinus ticks from north-western Poland with the use of PCR
    Autorzy:
    Sroka, J
    Wojcik-Fatla, A.
    Zwolinski, J.
    Zajac, V.
    Sawczuk, M.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51615.pdf
    Data publikacji:
    2008
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    polymerase chain reaction
    parasite
    genotyping
    Polska
    tick
    toxoplasmosis
    Ixodes ricinus
    B1 gene
    zoonotic disease
    occurrence
    Toxoplasma gondii
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2008, 15, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A small scale survey of Leptospira in mammals from eastern Poland
    Autorzy:
    Wojcik-Fatla, A.
    Zajac, V.
    Sroka, J.
    Piskorski, M.
    Cisak, E.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51664.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 4
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Environmental conditioning of incidence of tick-borne encephalitis in the South Eastern Poland in 1996-2006
    Autorzy:
    Bartosik, K.
    Lachowska-Kotowska, P.
    Szymanska, J.
    Wojcik-Fatla, A.
    Pabis, A.
    Buczek, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/49308.pdf
    Data publikacji:
    2011
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2011, 18, 1
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks determined by polymerase chain reaction with two pairs of primers detecting 16S rRNA and ankA genes
    Autorzy:
    Chmielewska-Badora, J
    Zwolinski, J.
    Cisak, E.
    Wojcik-Fatla, A.
    Buczek, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51023.pdf
    Data publikacji:
    2007
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Lublin region
    Polska
    pathogen
    Ixodes ricinus
    Eastern Poland
    tick
    determination
    animal pathogen
    human pathogen
    ankA gene
    16S rRNA gene
    granulocytic anaplasmosis
    bacteria
    Anaplasma phagocytophilum
    polymerase chain reaction
    Opis:
    A total of 684 Ixodes ricinus ticks (321 nymphs, 184 males, and 179 females) were collected by fl agging lower vegetation in 6 forest districts located on the territory of Lublin province (eastern Poland). Ticks were examined by polymerase chain reaction (PCR) method for the presence of Anaplasma phagocytophilum DNA with two pairs of primers: EHR521/EHR747 for detecting 16S rRNA gene, and LA6/LA1 for detecting ankA gene. To study the relationship between infection in ticks and people occupationally exposed to tick bite, blood serum samples of 261 forestry workers employed in the same forest districts were examined by immunofl uorescence method for the presence of specifi c antibodies against A. phagocytophilum. A total of 70 ticks out of 684 examined (10.2%) showed the presence of A. phagocytophilum 16S rRNA gene. The prevalence of infection was signifi cantly dependent on tick’s stage (χ2=49.2, p<0.00001) and geographical locality (χ2=34.4, p<0.00001). The percentage of I. ricinus females infected with A. phagocytophilum (24.6%) was signifi cantly greater compared to males (6.5%) and nymphs (4.4%) (p<0.00001). Only 19 ticks out of 684 examined (2.8%) showed the presence of A. phagocytophilum ankA gene, signifi cantly less compared to 16S rRNA gene (p<0.00001). The prevalence of infection demonstrated by the presence of ankA gene was also signifi cantly dependent on tick’s stage (χ2=23.6, p<0.00001) but not on locality (χ2=9.8, p=0.082). A signifi cant correlation was found between the presence of A. phagocytophilum 16S rRNA gene in I. ricinus female ticks from the particular forest districts and the serologic response to A. phagocytophilum of forestry workers employed in the same districts (p<0.05). No signifi cant correlation was found between the presence of A. phagocytophilum ankA gene in I. ricinus ticks and serologic response of exposed workers. In conclusion, detection of A. phagocytophilum infection in ticks by PCR with the use of EHR521/EHR747 primers detecting 16S rRNA gene is signifi cantly more sensitive compared to LA6/LA1 primers detecting ankA gene.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2007, 14, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occurrence of Leptospira DNA in water and soil samples collected in eastern Poland
    Autorzy:
    Wojcik-Fatla, A.
    Zajac, V.
    Wasinski, B.
    Sroka, J.
    Cisak, E.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51299.pdf
    Data publikacji:
    2014
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2014, 21, 4
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mycobiota and mycotoxins in various kinds of vegetables and fruits as potential health risk factors for consumers – summary of a multiyear study
    Autorzy:
    Kłapeć, T.
    Wójcik-Fatla, A.
    Farian, E.
    Kowalczyk, K.
    Cholewa, G.
    Cholewa, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/28761819.pdf
    Data publikacji:
    2022
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2022, 29, 2; 316-320
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mycobiota of berry fruits - levels of filamentous fungi and mycotoxins, composition of fungi, and analysis of potential health risk for consumers
    Autorzy:
    Kłapeć, T.
    Wójcik-Fatla, A.
    Farian, E.
    Kowalczyk, K.
    Cholewa, G.
    Cholewa, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/28761878.pdf
    Data publikacji:
    2022
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2022, 29, 1; 28-37
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Levels of filamentous fungi and selected mycotoxins in leafy and fruit vegetables and analysis of their potential health risk for consumers
    Autorzy:
    Kłapeć, T.
    Wójcik-Fatla, A.
    Farian, E.
    Kowalczyk, K.
    Cholewa, G.
    Cholewa, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/28762517.pdf
    Data publikacji:
    2021
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2021, 28, 4; 585-594
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A small-scale survey of hantavirus in mammals from eastern Poland
    Autorzy:
    Wojcik-Fatla, A.
    Zajac, V.
    Knap, J.P.
    Sroka, J.
    Cisak, E.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51577.pdf
    Data publikacji:
    2013
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    hantavirus
    Bunyaviridae
    mammal
    small mammal
    Microtus agrestis
    Myodes glareolus
    Sorex araneus
    epidemiology
    polymerase chain reaction
    flood
    Polska
    Opis:
    Samples of 30 dead small mammals each were collected on area ‘A’ located in eastern Poland which is exposed to flooding by the Vistula river, and on the area ‘B’, also located in eastern Poland but not exposed to flooding. Kidneys and livers of the mammals were examined by the PCR and nested PCR methods for the presence of hantavirus RNA. Out of 7 species of small mammals examined, the presence of hantaviruses was detected in 4 of them. Hantavirus prevalence was low in Apodemus agrarius (2.6%), the most numerous mammal species, whereas in the remaining 3 positive species (Microtus agrestis, Myodes glareolus, Sorex araneus) this was 12.5–100%. The presence of hantaviruses was detected only in the animals found on area ‘A’ exposed to flooding, and their prevalence was statistically greater compared to area ‘B’ not exposed to flooding (16.7% vs. 0%, p=0.0345). The overall positivity of the examined small mammals population from the areas ‘A’ and ‘B’ was 8.3%. The sequence analysis of the samples positive for hantavirus proved that the amplified products showed 77–86% homology with the L segment sequence of hantavirus Fusong-Mf-731 isolated from Microtus fortis in China. The presented study is the first to demonstrate the occurrence of hantavirus infection in small mammals from eastern Poland, and the first to demonstrate the significant relationship between flooding and the prevalence of hantaviruses in small mammals.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2013, 20, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on Lyme borreliosis focus in the Lublin region [eastern Poland]
    Autorzy:
    Cisak, E
    Chmielewska-Badora, J.
    Zwolinski, J.
    Wojcik-Fatla, A.
    Zajac, V.
    Skorska, C.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/51535.pdf
    Data publikacji:
    2008
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    borreliosis
    Polska
    rural inhabitant
    Lyme borreliosis
    tick
    seroepidemiology
    Ixodes ricinus
    Borrelia burgdorferi
    clinical case
    Lublin region
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2008, 15, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Associations between overweight and obesity and health enhancing behaviours among female nurses in Poland
    Autorzy:
    Woynarowska-Sołdan, M.
    Panczyk, M.
    Iwanow, L.
    Gałązkowski, R.
    Wójcik-Fatla, A.
    Panasiuk, L.
    Gotlib, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081941.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    health behaviours
    lifestyle
    obesity
    overweigh
    nurses
    Opis:
    Introduction and objective. The nursing profession entails many negative factors and high risk of chronic diseases, including overweight and obesity. The aim of the study is to investigate the prevalence of overweight and obesity and health enhancing behaviours in Polish female nurses, and associations between overweight and 4 groups of such behaviours, age and shift work. Materials and method. The analysis covered data obtained through cross-sectional survey carried out in a group of 994 nurses with an average age of 43. The analysis covered answers about 29 health-enhancing behaviours divided into 4 categories (subscales): 1) nutrition, 2) physical activity, 3) sleep, rest and behaviours related to mental health, 4) preventive behaviours. They were analysed through the validated Positive Health Behaviours Scale for adults. The analysis also covered answers about avoiding drinking large amounts of alcohol in one go, not smoking, avoiding passive smoking, not abusing unprescribed drugs. Results. The prevalence of overweight and obesity was 44%. Of 29 health behaviours concerned with nutrition, physical activity, sleep, rest, and mental health, preventive behaviours, 3 were exhibited always or almost always by over a half of the nurses. Health behaviours were more common in nurses whose BMI was normal than in nurses with BMI ≥ 25.0. Age and low physical activity levels were related to overweight and obesity in nurses. Conclusions. The study revealed a high prevalence of overweight and obesity and many deficits in health behaviours. Obesity and health behaviour deficits pose risks to the health of nurses, limit their involvement in prevention and treatment of patient obesity and impact as health educators. It is essential to engage in actions for health promotion among nurses.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 714-719
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Streptococcus suis: a re-emerging pathogen associated with occupational exposure to pigs or pork products. Part II – pathogenesis
    Autorzy:
    Dutkiewicz, J.
    Zając, V.
    Sroka, J.
    Wasiński, B.
    Cisak, E.
    Sawczyn, A.
    Kloc, A.
    Wojcik-Fatla, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081593.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Streptococcus suis
    pathogenesis
    infection stages
    interaction with host cells and tissues
    virulence factors
    genomic
    determinants of pathogenicity
    biofilm formation
    Opis:
    Abstract Streptococcus suis is a re-emerging zoonotic pathogen that may cause severe disease, mostly meningitis, in pigs and in humans having occupational contact with pigs and pork, such as farmers, slaughterhose workers and butchers. The first stage of the pathogenic process, similar in pigs and humans, is adherence to and colonisation of mucosal and/or epithelial surface(s) of the host. The second stage is invasion into deeper tissue and extracellular translocation of bacterium in the bloodstream, either free in circulation or attached to the surface of monocytes. If S. suis present in blood fails to cause fatal septicaemia, it is able to progress into the third stage comprising penetration into host’s organs, mostly by crossing the blood-brain barrier and/or blood–cerebrospinal fluid barrier to gain access to the central nervous system (CNS) and cause meningitis. The fourth stage is inflammation that plays a key role in the pathogenesis of both systemic and CNS infections caused by S. suis. The pathogen may induce the overproduction of pro-inflammatory cytokines that cause septic shock and/or the recruitment and activation of different leukocyte populations, causing acute inflammation of the CNS. Streptococcus suis can also evoke – through activation of microglial cells, astrocytes and possibly other cell types – a fulminant inflammatory reaction of the brain which leads to intracranial complications, including brain oedema, increased intracranial pressure, cerebrovascular insults, and deafness, as a result of cochlear sepsis. In all stages of the pathogenic process, S. suis interacts with many types of immunocompetent host’s cells, such as polymorphonuclear leukocytes, mononuclear macrophages, lymphocytes, dendritic cells and microglia, using a range of versatile virulence factors for evasion of the innate and adaptive immune defence of the host, and for overcoming environmental stress. It is estimated that S. suis produces more than 100 different virulence factors that could be classified into 4 groups: surface components or secreted elements, enzymes, transcription factors or regulatory systems and transporter factors or secretion systems. A major virulence factor is capsular polysaccharide (CPS) that protects bacteria from phagocytosis. However, it hampers adhesion to and invasion of host’s cells, release of inflammatory cytokines and formation of the resistant biofilm which, in many cases, is vital for the persistence of bacteria. It has been demonstrated that the arising by mutation unencapsulated S. suis clones, which are more successful in penetration to and propagation within the host’s cells, may coexist in the organism of a single host together with those that are encapsulated. Both ‘complementary’ clones assist each other in the successful colonization of host’s tissues and persistence therein. S. suis has an open pan-genome characterized by a frequent gene transfer and a large diversity. Of the genetic determinants of S. suis pathogenicity, the most important are pathogenicity islands (PAI), in particular, a novel DNA segment of 89 kb length with evident pathogenic traits that has been designated as 89K PAI. It has been estimated that more than one-third of the S. suis virulence factors is associated with this PAI. It has been proved that the virulent S. suis strains possess smaller genomes, compared to avirulent ones, but more genes associated with virulence. Overall, the evolution of the species most probably aims towards increased pathogenicity, and hence the most significant task of the current research is an elaboration of a vaccine, efficient both for humans and pigs.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 1; 186-203
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Microbial contamination of money sorting facilities
    Autorzy:
    Górny, R.L.
    Gołofit-Szymczak, M.
    Wójcik-Fatla, A.
    Cyprowski, M.
    Stobnicka-Kupiec, A.
    Ławniczek-Wałczyk, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/28762870.pdf
    Data publikacji:
    2021
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2021, 28, 1; 61-71
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Seroprevalence of leptospirosis in rural populations inhabiting areas exposed andnot exposed to floods in eastern Poland
    Autorzy:
    Wasinski, B.
    Sroka, J.
    Wojcik-Fatla, A.
    Zajac, V.
    Cisak, E.
    Knap, J.P.
    Sawczyn, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/50328.pdf
    Data publikacji:
    2012
    Wydawca:
    Instytut Medycyny Wsi
    Opis:
    Blood serum samples collected from randomly selected groups of 100 persons inhabiting rural community ‘A’ located in eastern Poland and exposed to floods by the Vistula river, and 98 persons inhabiting rural community ‘B’, also located in eastern Poland, but in the area not exposed to floods were examined by the microscopic agglutination test (MAT) for the presence of antibodies against 18 Leptospira serovars. Positive results showed 3% of serum samples collected from community ‘A’, while the seroprevalence in community ‘B’ was 9.2%, being insignificantly higher than in community ‘A’. For both examined communities (n=198), a significant positive correlation was found between the prevalence and titer of seropositive response and age of examined people (r=0.145, p=0.042). No significant differences were found between the prevalence of positive reactions in males and females (p>0.05). The reactions with 10 serovars of Leptospira (Australis, Autumnalis, Hebdomadis, Hardjo, Sejroe, Zanoni, Bataviae, Bratislava, Canicola and Grippotyphosa), belonging to 3 species (L. interrogans, L. borgpetersenii, L. kirschneri), were found in the examined communities. From both communities, of 12 persons demonstrating positive results in MAT, 9 showed reaction with one serovar, 2 with two serovars and 1 with three serovars. The highest titers found during the examination did not exceeded 800. In conclusion, our results suggest that there is only a slight, if any, hazard of an leptospirosis epidemic after the flood that afflicted eastern Poland in the year 2010 and the general epidemiological situation of leptospirosis in eastern Poland. Although there does not seem to be any cause for concern, further research is needed.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2012, 19, 2
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Ocena narażenia populacji wiejskiej Lubelszczyzny na zakażenie krętkami z rodzaju Leptospira, ze szczególnym uwzględnieniem terenów popowodziowych
    Assessment of risk of infection with Leptospira spirochetes among rural population in the Lublin Region, with particular consideration of areas exposed to flooding
    Autorzy:
    Dutkiewicz, J.
    Wojcik-Fatla, A.
    Zajac, V.
    Wasinski, B.
    Knap, P.J.
    Sroka, J.
    Cisak, E.
    Sawczyn, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/4449.pdf
    Data publikacji:
    2015
    Wydawca:
    Instytut Medycyny Wsi
    Opis:
    Wprowadzenie. Leptospiroza, wywoływana przez krętki z rodzaju Leptospira, jest uważana za najbardziej rozprzestrzenioną chorobę odzwierzęcą na świecie. Zapadalność może być zwiększona przez klęski żywiołowe takie jak powodzie i tajfuny. Cel pracy. Celem pracy było zbadanie sytuacji epidemiologicznej leptospirozy na Lubelszczyźnie (wschodnia Polska), z uwzględnieniem wpływu powodzi Wisły, poprzez badania populacji ludzkiej i różnych elementów środowiska na obecność Leptospira w dwóch rejonach: rejonie „A” nawiedzanym przez powodzie i rejonie „B” nienawiedzanym przez powodzie. Materiał i metody. W rejonach „A” i „B” przebadano surowice, odpowiednio 100 i 98 mieszkańców, surowice 32 i 41 świń oraz surowice 41 i 40 krów – na obecność przeciwciał anty- Leptospira, wykorzystując test aglutynacji mikroskopowej (MAT). We wskazanych rejonach przebadano również, odpowiednio 40 i 64 próbki wody, 40 i 68 próbek gleby, próbki organów 30 i 30 drobnych ssaków należących do 5 i 6 gatunków, a także 540 i 296 kleszczy Ixodes ricinus – na obecność DNA Leptospira; badanie przeprowadzono testem nested-PCR. Wyniki. Obecność przeciwciał anty-Leptospira stwierdzono u 3% mieszkańców rejonu „A” i u 9,2% mieszkańców rejonu „B”; różnica ta nie była statystycznie znamienna. Częstość występowania przeciwciał anty-Leptospira była większa u świń i krów z rejonu „A” w porównaniu z rejonem „B” (odpowiednio 34,4% wobec 4,9%, i 26,8% wobec 15,0%), a w przypadku świń różnica okazała się znamienna (P=0,0015). Także miana dodatnich reakcji były wyższe w rejonie „A” w porównaniu z „B”, a w przypadku krów różnica okazała się znamienna (P=0,0128). Obecność DNA Leptospira spp. stwierdzono u 20% drobnych ssaków z rejonu „A” i u 30% z rejonu „B”; różnica ta nie była znamienna, chociaż częstość dodatnich wyników była w obu przypadkach wysoka w porównaniu z danymi z piśmiennictwa. Przeważającą część wyników dodatnich uzyskano u myszy polnych (Apodemus agrarius). Obecność DNA Leptospira spp. wykryto u 15,6% kleszczy I. ricinus z rejonu „A” w porównaniu z 1,4% w rejonie „B”, a różnica ta okazała się wysoce znamienna (P<0,0001). DNA Leptospira spp. zawierało 5% próbek wody z rejonu „A”, podczas gdy wszystkie próbki wody z rejonu „B” oraz wszystkie próbki gleby z rejonów „A” i „B” były ujemne. Wnioski. • Zakażenia krętkami Leptospira występujące u ludności wiejskiej zamieszkującej tereny Lubelszczyzny nawiedzane przez powodzie Wisły nie są częstsze w porównaniu z ludnością zamieszkującą inne części regionu nienawiedzane przez powodzie. • Potencjalnym źródłem zakażeń leptospirami na terenach nawiedzanych przez powodzie są zwierzęta: kleszcze Ixodes ricinus, zwierzęta hodowlane (świnie, krowy) i myszy polne (Apodemus agrarius). • Wykrycie DNA Leptospira spp. w 5% próbek wody z terenów popowodziowych, przy braku pozytywnych wyników z terenu kontrolnego, wydaje się potwierdzać możliwą rolę wody w szerzeniu leptospirozy. • Gleba nie stanowi istotnego zagrożenia na terenach nawiedzanych przez powodzie.
    Background. Leptospirosis, caused by Leptospira spirochetes, is considered the most widespread zoonosis worldwide. Morbidity may be increased by natural disasters such as floods or typhoons. Aim of the study. The aim of the present study was to investigate the epidemiological situation of leptospirosis in the Lublin Region (Eastern Poland) with consideration of the effects of flooding by the Vistula River, by investigating human population and various elements of the environment for the presence of Leptospira in two areas: ‘Area A’ exposed to flooding, and ‘Area B’ not exposed to flooding. Material and Methods. In the Areas A and B, sera of 100 and 98 inhabitants, sera of 32 and 41 pigs and sera of 41 and 40 cows were examined, respectively, for the presence of anti-Leptospira antibodies by the microscopic agglutination test (MAT), as well as 40 and 64 samples of water, 40 and 68 samples of soil, organ samples of 30 and 30 small mammals from 5 and 6 species, and 540 and 296 Ixodes ricinus ticks, respectively – for the presence of Leptospira DNA by the nested-PCR test. Results. The presence of anti-Leptospira antibodies was found in 3% of inhabitants of Area A and in 9.2% of Area B; this difference was statistically insignificant. The frequency of anti-Leptospira antibodies was higher in pigs and cows from Area A, compared to area B (34.4% vs. 4.9%, and 26.8% vs. 15.0%, respectively), while in the case of pigs the difference was significant (P=0.0015). Also, the titers of positive reactions were higher in Area A, compared to Area B, and for cows the difference was significant (P=0.0128). The presence of Leptospira DNA was found in 20% of small mammals from Area A, and in 30% from Area B; this difference being insignificant; however, in both cases the frequency of positive results was high, compared to the data from literature. The great majority of positive results were obtained in striped field mice (Apodemus agrarius). The presence of Leptospira DNA was detected in 15.6% of I. ricinus ticks from Area A, compared to 1.4% in Area B, and the difference was highly significant (P<0.0001). 5% of water samples from Area A contained Leptospira DNA, whereas all water samples from Area B, and all soil samples from Areas A and B were negative. Conclusions. • The infections with Leptospira spirochetes among the rural population inhabiting the areas of the Lublin Region exposed to floods by the Vistula River do not occur with a higher frequency, compared to the areas not exposed to floods. • The potential sources of infection with Leptospira in the areas exposed to flooding are animals: ticks Ixodes ricinus, domestic animals (pigs, cows), and striped field mice (Apodemus agrarius). • Detection of Leptospira spp. DNA in 5% of water samples in the areas exposed to floods, with negative results in control areas, seems to confirm a possible role of water in spreading leptospirosis. • Soil does not constitute any significant risk of infection in the areas exposed to flooding.
    Źródło:
    Medycyna Ogólna i Nauki o Zdrowiu; 2015, 21(50), 1
    2083-4543
    Pojawia się w:
    Medycyna Ogólna i Nauki o Zdrowiu
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Is job seniority a protective factor against anxiety among midwives during the SARS-CoV-2 pandemic?
    Autorzy:
    Gotlib, J.
    Rzońca, E.
    Baranowska, B.
    Tataj-Puzyna, U.
    Pawlicka, P.
    Jaworski, M.
    Wójcik-Fatla, A.
    Panczyk, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/28762756.pdf
    Data publikacji:
    2021
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2021, 28, 2; 352-357
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Toxoplasma gondii infection in selected species of free-living animals in Poland
    Autorzy:
    Sroka, J.
    Karamon, J.
    Wójcik-Fatla, A.
    Dutkiewicz, J.
    Bilska-Zając, E.
    Zając, V.
    Piotrowska, W.
    Cencek, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085130.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Polska
    Toxoplasma gondii
    nested PCR
    genotyping
    free-living animals
    Opis:
    Introduction and objective. Free-living animals can play an important role as a reservoir of Toxoplasma gondi;, however, data concerning this issue in Poland are still limited.The aim of study was to assess the occurrence of T. gondii infection by using molecular methods in free-living animals in selected regions of Poland. Materials and method. Tissues samples of 396 animals (foxes, muskrats, birds, martens, badgers, polecats, raccoons, minks, raccoon dogs, otters, small rodents and insectivores, and grass snakes were collected from various regions of Poland. After samples digestion, DNA was isolated using QIAmp DNA Mini Kit (Qiagen). DNA extraction from small rodents and insectivores samples was performed without digestion. Next, nested PCR (B1 gene) and, for a part of nested PCR positive amplicons, RFLP PCR, were performed according to the method by Grigg and Boothroyd (2001). The other part of nested PCR positive DNA isolates were genotyped using 5 genetic markers: SAG1, SAG2 (5’- and 3’), SAG3, BTUB and GRA6, based on the method by Dubey et al. (2006). These PCR products were sequenced and compared with the NCBI database using Blast. Results. In total, in 50 of the 396 examined animals DNA of T. gondii was detected (12.6%). The highest percentages of positive results in PCR was obtained in martens (40.9%) and badgers (38.5%), lower in birds (27.3%) and the lowest in foxes (7.4%). The RFLP and multilocus PCR analysis showed the dominance of T. gondii clonal type II (or II/III). Conclusions. The results of this study indicate the frequent T. gondii infection among free-living animals in Poland, especially martens and badgers, which may indirectly indicate that these animals contribute to the spread of the parasite in the sylvatic environment in Poland. The genotyping analysis showed the dominance of T. gondii clonal type II (or II/III).
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 656-660
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Study on prevalence of Toxoplasma gondii infection in free-living animals in Poland
    Autorzy:
    Sroka, J.
    Karamon, J.
    Bilska-Zajac, E.
    Stojecki, K.
    Rozycki, M.
    Kusyk, P.
    Wojcik-Fatla, A.
    Zajac, V.
    Cencek, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/6474.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Parazytologiczne
    Tematy:
    Toxoplasma gondii
    animal infection
    prevalence
    free living animal
    Polska
    Źródło:
    Annals of Parasitology; 2016, 62, Suppl.
    0043-5163
    Pojawia się w:
    Annals of Parasitology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Potential sources of infection with selected zoonotic agents in the veterinary work environment - pilot studies
    Autorzy:
    Wójcik-Fatla, A.
    Sroka, J.
    Zając, V.
    Sawczyn-Domańska, A.
    Kloc, A.
    Zwoliński, J.
    Kłapeć, T.
    Studzińska, M.B.
    Chmura, R.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085448.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    environment
    Coxiella burnetii
    Toxoplasma gondii
    veterinarians
    Leptospira spp
    Cryptosporidium spp
    Giardia duodenalis
    Opis:
    Introduction and Objective. The problem of occupational biohazards is very important, especially in the field of agriculture and in human and veterinary medicine. The aim of the study was to determine the potential sources of infection in veterinary professionals with selected zoonotic agents, including: Toxoplasma gondii, Giardia duodenalis, Leptospira spp., Cryptosporidium spp. and Coxiella burnetii. Materials and Method. A total of 50 air samples from barns, piggeries and veterinary surgeries were examined for the presence of Leptospira spp. and C. burnetii DNA. Serum samples of 86 pigs and 80 cows were tested for the presence of antibodies to Leptospira spp. and to phase I and II C. burnetii antigens. Serum of 70 cats were tested for the presence of antibodies to T. gondii and 65 samples of cat faeces for the presence of T. gondii oocysts. The presence of G. duodenalis and Cryptosporidium spp. were examined in 50 of dog faeces and 50 of bovine faeces samples. Results. DNA of Leptospira spp. was detected in 2 air samples from the piggeries (4%). C. burnetii DNA was not found in any sample. Anti-Leptospira spp. antibodies were detected in 51 (59.3%) of examined pigs. Neither anti-Leptospira spp. nor anti-C. burnetii antibodies were found among samples of bovine serum. Anti-T. gondii antibodies was found in 52 cat serum samples (74.3%). Among samples of cat faeces, no T. gondii oocysts were detected. In one sample of cattle stool (2%), G. duodenalis was detected and in another (2%) – Cryptosporidium spp. G. duodenalis was detected in 7 samples (14%) and Cryptosporidium spp. in 2 samples (2%) of dog faeces. Conclusions. The results of this study demonstrate the potential risk of infection with Leptospira spp. in veterinarians working with pigs. Veterinarians could be also be at risk of infection with T. gondii and G. duodenalis.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2020, 27, 1; 146-150
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in Poland
    Autorzy:
    Gora, A
    Mackiewicz, B.
    Krawczyk, P.
    Golec, M.
    Skorska, C.
    Sitkowska, J.
    Cholewa, G.
    Larsson, L.
    Jarosz, M.
    Wojcik-Fatla, A.
    Dutkiewicz, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/50063.pdf
    Data publikacji:
    2009
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    occupational exposure
    organic dust
    dust
    microorganism
    endotoxin
    plant processing
    worker
    Polska
    peptidoglycan
    bioaerosol
    agricultural industry
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 143-150
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparison of the effectiveness of various parasitological methods in detecting nematode eggs in different types of soil
    Autorzy:
    Zdybel, J.M.
    Sroka, J.
    Karamon, J.
    Bilska-Zając, E.
    Wójcik-Fatla, A.
    Kłapeć, T.
    Skowron, P.
    Siebielec, G.
    Jadczyszyn, T.
    Cencek, T.
    Powiązania:
    https://bibliotekanauki.pl/articles/28763034.pdf
    Data publikacji:
    2023
    Wydawca:
    Instytut Medycyny Wsi
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2023, 30, 3; 425-431
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Acute hypersensitivity pneumonitis in woodworkers caused by inhalation of birch dust contaminated with Pantoea agglomerans and Microbacterium barkeri
    Autorzy:
    Mackiewicz, B.
    Dutkiewicz, J.
    Siwiec, J.
    Kucharczyk, T.
    Siek, E.
    Wójcik-Fatla, A.
    Cholewa, G.
    Cholewa, A.
    Paściak, M.
    Pawlik, K.
    Szponar, B.
    Milanowski, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085105.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    hypersensitivity pneumonitis
    wood dust
    bacteria
    Pantoea agglomerans
    Microbacterium barkeri
    inhalation challenge
    occupational exposure
    Opis:
    Case description. Five workers (2 males and 3 females) employed in a furniture factory located in eastern Poland developed hypersensitivity pneumonitis (HP) after the pine wood used for furniture production was replaced by birch wood. All of them reported onset of respiratory and general symptoms (cough, shortness of breath, general malaise) after inhalation exposure to birch dust, showed crackles at auscultation, ground-glass attenuations in HRCT examination, and lymphocytosis in the BAL examination. The diagnosis of acute HP was set in 4 persons and the diagnosis of subacute HP in one. Identification of specific allergen. Samples of birch wood associated with evoking disease symptoms were subjected to microbiological analysis with the conventional and molecular methods. Two bacterial isolates were found to occur in large quantities (of the order 108 CFU/g) in examined samples: Gram-negative bacterium of the species Pantoea agglomerans and a non-filamentous Gram-positive actinobacterium of the species Microbacterium barkeri. In the test for inhibition of leukocyte migration, 4 out of 5 examined patients showed a positive reaction in the presence of P. agglomerans and 2 in the presence of M. barkeri. Only one person showed the presence of precipitins to P. agglomerans and none to M. barkeri. In the inhalation challenge, which is the most relevant allergological test in the HP diagnostics, all patients reacted positively to P. agglomerans and only one to M. barkeri. The results indicate that P. agglomerans developing in birch wood was the main agent causing HP in the workers exposed to the inhalation of dust from this wood, while the etiologic role of M. barkeri is probably secondary. Conclusion. The results demonstrate that apart from fungi and filamentous actinobacteria, regarded until recently as causative agents of HP in woodworkers, Gram-negative bacteria and non-filamentous actinobacteria may also elicit disease symptoms in the workers processing wood infected with large amounts of these microorganisms. The results obtained also seem to indicate that cellular-mediated reactions are more significant for causing disease symptoms compared to those that are precipitin-mediated.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 644-655
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-51 z 51

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