A total of 123 water samples were examined in parallel by culture and semi-nested PCR for the presence of Legionella. They
comprised: 35 samples of hot water distributed by the urban municipal water supply system (MWSS) taken in institutions,
45 samples of hot water distributed by urban MWSS taken in dwellings, 27 samples of cold water distributed by rural MWSS
taken in dwellings, and 16 samples of cold well water taken in rural areas. The greatest frequency of the isolation of Legionella
by culture (88.6%) was recorded in the samples of hot water from the urban institutions, having been greater compared to
all other sources (p<0.001). The frequency of Legionella isolation from hot water in urban dwellings (28.9%) was significantly
greater compared to the combined value (2.3%) for cold water from rural MWSS and wells (p<0.001). Strains belonging to
Legionella pneumophila serogroups 2-14 predominated in the examined samples, while strains of L. pneumophila serogroup
1 and strains of Legionella spp. (other than L. pneumophila) were 3-fold less numerous. The rates of positive findings in the
semi-nested PCR (stage 2) were greater than culture isolations in all kinds of samples, except for urban institutions. The
correlation between the culture and PCR results was positive for samples of hot water from urban MWSS (p<0.01), but not
for samples of cold water from rural MWSS and wells (p>0.5). A significant correlation was found between rates of PCRpositive
results and numbers of Legionella pneumophila serogroups 2-14 strains, but not for other Legionella serogroups or
species. In conclusion, our results support the opinion that though PCR cannot be a substitute for the isolation of Legionella
by culture, it could be regarded as an useful complementary method.
Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies
Informacja
SZANOWNI CZYTELNICY!
UPRZEJMIE INFORMUJEMY, ŻE BIBLIOTEKA FUNKCJONUJE W NASTĘPUJĄCYCH GODZINACH:
Wypożyczalnia i Czytelnia Główna: poniedziałek – piątek od 9.00 do 19.00