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Tytuł:
Analysis and experiments of embedded gripping mechanism used in large-scale tools holding up pile foundation in ocean
Autorzy:
Wang, Zhuo
Zhang, Bo
Wang, Tao
Powiązania:
https://bibliotekanauki.pl/articles/259156.pdf
Data publikacji:
2019
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
pile foundation
gripping
embedded mechanism
embedded depth
upending gripper
Opis:
With the rapid development of the marine economy and continuous improvement of the industry, the scale of the offshore engineering is increasing. This raises interest in studying, theoretically and experimentally, gripping and bearing mechanisms for large-scale holding and lifting tools used in foundation pile installations. In this paper, the embedded gripping mechanism is studied based on the theory of elastic-plastic mechanics. The embedded and bearing performance of the tooth is simulated and the influence factors are studied. In addition, the device used in the simplified embedded experiment on the tooth of the embedded block is designed. The relationship between embedded depth, load, and tooth profile angle is identified and validated. Meanwhile, the embedded performance of linear and ring type teeth is compared experimentally in order to select the suitable type of tooth for various situations. This comparison makes the basis for designing an upending gripper for the marine pile foundation, which can realize the operation of holding the pile to prevent its falling.
Źródło:
Polish Maritime Research; 2019, 3; 15-21
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of Annealing Treatment on Deep Drawing Behavior of Q235 Carbon Steel /410/304 Stainless Steels Three-Layer Composite Plate
Autorzy:
Lv, Zehua
Zhang, Zhixiong
Han, Jianchao
Wang, Tao
Powiązania:
https://bibliotekanauki.pl/articles/2106574.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
composite plate
deep drawing
annealing
microstructure
Opis:
Effect of annealing treatment on deep drawing behavior of hot-rolled Q235 carbon steel/410/304 stainless steel three-layer composite plate was investigated. Deep drawability of the unannealed composite plates exhibits a sharp difference for various contact surfaces with the die. The limit drawing ratio (LDR) of the composite plate with the carbon steel contacting the die is 1.75, while it is 1.83 with the stainless steel contacting the die due to the different mechanical responses to the tensile stress at the corner of the die. After annealing at 900°C for 2 h, however, the deep drawabilities of the composite plates both for various contact surfaces with the die are significantly improved and becomes almost identical, which are attributed to the stress relief, the enhanced ductility and the improved interface bonding strength of the hot-rolled component plates during annealing.
Źródło:
Archives of Metallurgy and Materials; 2022, 67, 2; 421--433
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Light waves scattering from an anisotropic semi-soft boundary medium with spectral dependence
Autorzy:
Huang, Zhiru
Dai, Qihang
Ji, Xiaoling
Wang, Tao
Powiązania:
https://bibliotekanauki.pl/articles/1835777.pdf
Data publikacji:
2021
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
light scattering
spectral dependent medium
spectral density
spectral degree of coherence
Opis:
The far-zone behavior of polychromatic light waves on scattering from an anisotrophic semi-soft boundary medium with spectral dependence was considered, and the spectral density and the spectral degree of coherence of the far-zone scattered field were investigated. It is shown that the distributions of the spectral density and the spectral degree of coherence of scattered field are closely related with the rms width, the center wavelength, and the maximum value of the center wavelength of the scattering potential of the scattering medium.
Źródło:
Optica Applicata; 2021, 51, 2; 271-280
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Manipulating far-field ring-shaped array according to the superposition of weight functions
Autorzy:
Yuan, Chenyuan
Yuan, Caifu
Xiang, Sufen
Ji, Xiaoling
Wang, Tao
Powiązania:
https://bibliotekanauki.pl/articles/2202770.pdf
Data publikacji:
2023
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
partially coherent source
optical manipulation
ring-shaped array
spectral density
Opis:
In order to control the distribution characteristics of the far-field ring-shaped array, we introduce a new light source to produce adjustable far-field distribution by the method of weight function superposition. It has been shown that, by changing the parameters of the light source, one can obtain far-field with various distribution, including distribution with decrease in spectral intensity of specified rings, distribution with disappearances of specified rings, distribution with different spectral intensity of part of lobes in the continuous rings, distribution with part of the lobes in specified rings disappearing and distribution with some lobes in specified rings being stronger. These results will produce some novel far-field distributions which may provide a new idea for further study concerning about the manipulations of far-field array distribution.
Źródło:
Optica Applicata; 2023, 53, 1; 87--98
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ultrasonic Assisted Active-Passive Filling Friction Stir Repairing to Eliminate Volume Defects
Autorzy:
Wang, Tao
Gong, Xue
Ji, Shude
Xue, Gang
Lv, Zan
Powiązania:
https://bibliotekanauki.pl/articles/2049431.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
friction stir repairing
magnesium alloys
defects
ultrasonic
mechanical properties
Opis:
Ultrasonic assisted active-passive filling friction stir repairing (A-PFFSR) was proposed to repair volume defects in the metallic parts. Sound joints without interfacial defects could be achieved. Firstly, the ultrasonic was beneficial to improving material flow and atom diffusion, and then eliminated kissing bond defects compared to conventional A-PFFSR joints. Secondly, the equiaxed grains were refined by ultrasonic vibration. Lastly, the repairing passes were reduced due to the ultrasonic, which decreased softening degree of the repaired joints. The maximum tensile strength of 150 MPa was achieved. Therefore, this strategy to repair the volume defects is feasibility and potential in the remanufacturing fields of aerospace and transportation.
Źródło:
Archives of Metallurgy and Materials; 2021, 66, 1; 217-222
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The far-zone behaviors of light waves on scattering from particulate medium with various distribution
Autorzy:
Dai, Qihang
Xie, Zhongyuan
Pan, Xiaoning
Ji, Xiaoling
Wang, Tao
Powiązania:
https://bibliotekanauki.pl/articles/2086763.pdf
Data publikacji:
2022
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
weak scattering
particulate collection
spectral density
Opis:
The far-zone spectral density of light waves scattered on a particulate medium was discussed, and the influence of characteristics of the medium on the far-zone scattered spectral density was investigated. It is shown that the normalized spectral density of the scattered field is closely related with the structural characteristics of the particles collection, including the relative size of particles and the distribution information of particles in the collection. These results may provide potential application in the reconstruction of the structure information of particulate medium.
Źródło:
Optica Applicata; 2022, 52, 2; 273--282
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of the influence of warning sounds in expressway tunnels on the mental state and attention of the driver
Autorzy:
Xiao, Yao
Liang, Bo
Wang, Tao
Niu, Jiaan
He, Shiyong
Powiązania:
https://bibliotekanauki.pl/articles/27312133.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
dźwięk ostrzegawczy
tunel
droga ekspresowa
kierowca
stan psychiczny
charakterystyka wizualna
warning sound
expressway
tunnel
driver
mental state
visual characteristic
Opis:
In recent years, some expressway tunnels have started broadcasting warning sounds, such as fire alarms, to improve driver attention and traffic safety. However, there is few research on it, and in contrast to previous studies, we have considered different evaluation indices and through field measurement to determine the effectiveness of this practice. The characteristics of three warning sound signals, i.e., fire alarm, dynamic music, and voice command, in a tunnel were analyzed using MATLAB. Considering pupil diameter and blink duration as evaluation indexes, the change in the mental state of the driver after hearing a warning sound was analyzed. Based on Markov chain theory, the change in the gaze region and gaze shift of the driver under the condition of a warning sound was analyzed. Results shows fire alarms and voice commands can increase the mental load of drivers, but the degree of impact was not determined. Dynamic music does not affect the mental load of the driver. The fire alarm and dynamic music attracted the attention of the driver; conversely, as the voice command warns the driver to focus on safety, it did not impact the attention of the driver. The research results provide a scientific reference for the selection of warning sounds in expressway tunnels and new research ideas for the prevention of traffic injuries in expressway tunnels.
Źródło:
Archives of Civil Engineering; 2023, 69, 2; 623--636
1230-2945
Pojawia się w:
Archives of Civil Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Generalized PI observer design for descriptor linear system
Autorzy:
Wang, Xin-Tao
Yu, Hai-Hua
Powiązania:
https://bibliotekanauki.pl/articles/229300.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
generalized proportional-integral observer
Sylvester matrix equation
regularity
impulse-freeness
Opis:
A kind of generalized proportional-integral (GPI) observer for descriptor linear systems is introduced. We first propose two complete parametric solutions to generalized Sylvester matrix equation corresponding to the left eigenvector matrices in the case of Jordan form. Then a parametric design approach for the observer is presented. The proposed method provides all parametric expression of the gain matrices and the corresponding finite left eigenvector matrix and guarantees the regularity and impulse-freeness of the expanded error system. Two numerical examples are given to explain the design procedure and illustrate the effectiveness of the proposed method.
Źródło:
Archives of Control Sciences; 2019, 29, 4; 585-601
1230-2384
Pojawia się w:
Archives of Control Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
NEW BETULIN NITRATES: SYNTHESIS, CYTOTOXIC ACTIVITY AND MOLECULAR DOCKING EVALUATION
Autorzy:
Feng, GaiLi
Wang, Tao
Zhang, Rong
Luo, Jin
Xiao, MinJie
He, BaoEn
Liu, YongQian
Wu, JunXiao
Powiązania:
https://bibliotekanauki.pl/articles/895411.pdf
Data publikacji:
2018-10-31
Wydawca:
Polskie Towarzystwo Farmaceutyczne
Tematy:
Molecular docking
anti-tumor
Betulin nitrates
NO donors
Opis:
Betulin and its derivatives have been reported to affect several key genes of cell-cycle regulators. However, their specific targets haven’t yet been discovered. As an important cell cycle regulator, Cyclin-dependent kinase-2 (CDK2) has become a potential target for cancer therapy. Here we describe the design, synthesis and antitumor activities in vitro of eleven new betulin nitrates. The results revealed that compound (20) possesses potent antitumor activity against MCF-7 cell lines (IC50 < 10 μM). In order to investigate potential protein target, betulin nitrates were subjected to docking studies with CDK2. Compound (20) showed very good binding affinity for CDK2 via hydrogen bonding interactions. Thus, the CDK2 inhibitory potential could make compound (20) possible candidate as antitumor agent.
Źródło:
Acta Poloniae Pharmaceutica - Drug Research; 2018, 75, 5; 1135-1145
0001-6837
2353-5288
Pojawia się w:
Acta Poloniae Pharmaceutica - Drug Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Simulation studies on the influence of other combustible gases on the characteristics of methane explosions at constant volume and high temperature
Autorzy:
Luo, Zhenmin
Liu, Litao
Gao, Shuaishuai
Wang, Tao
Su, Bin
Wang, Lei
Yang, Yong
Li, Xiufang
Powiązania:
https://bibliotekanauki.pl/articles/1853863.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
gaz palny
ciśnienie wybuchu
temperatura płomienia
constant volume
volume fraction of combustible gas
initial temperature
adiabatic flame temperature
maximum explosion pressure
Opis:
Gas explosions are major disasters in coal mining, and they typically cause a large number of deaths, injuries and property losses. An appropriate understanding of the effects of combustible gases on the characteristics of methane explosions is essential to prevent and control methane explosions. FLACS software was used to simulate an explosion of a mixture of CH4 and combustible gases (C2H4, C2H6, H2, and CO) at various mixing concentrations and different temperatures (25, 60, 100, 140 and 180℃). After adding combustible gases to methane at a constant volume and atmospheric pressure, the adiabatic flame temperature linearly increases as the initial temperature increases. Under stoichiometric conditions (9.5% CH4-air mixture), the addition of C2H4 and C2H6 has a greater effect on the adiabatic flame temperature of methane than H2 and CO at different initial temperatures. Under the fuel-lean CH4-air mixture (7% CH4-air mixture) and fuel-rich mixture (11% CH4-air mixture), the addition of H2 and CO has a greater effect on the adiabatic flame temperature of methane. In contrast, the addition of combustible gases negatively affected the maximum explosion pressure of the CH4-air mixture, exhibiting a linearly decreasing trend with increasing initial temperature. As the volume fraction of the mixed gas increases, the adiabatic flame temperature and maximum explosion pressure of the stoichiometric conditions increase. In contrast, under the fuel-rich mixture, the combustible gas slightly lowered the adiabatic flame temperature and the maximum explosion pressure. When the initial temperature was 140℃, the fuel consumption time was approximately 8-10 ms earlier than that at the initial temperature of 25℃. When the volume fraction of the combustible gas was 2.0%, the consumption time of fuel reduced by approximately 10 ms compared with that observed when the volume fraction of flammable gas was 0.4%.
Źródło:
Archives of Mining Sciences; 2021, 66, 2; 279-295
0860-7001
Pojawia się w:
Archives of Mining Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of surfactants on the flotation performance of low-rank coal by particle sliding process measurements
Wpływ surfaktantów na sprawność flotacji węgla niskiej jakości w pomiarach procesu przemieszczania się cząstek
Autorzy:
Wang, S.
Tao, X.
Powiązania:
https://bibliotekanauki.pl/articles/216970.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Instytut Gospodarki Surowcami Mineralnymi i Energią PAN
Tematy:
węgiel o niskiej wartości
prędkość kątowa poślizgu
hydrofobowość
środek powierzchniowo czynny
wynik XPS
low rank coal
slip angle velocity
hydrophobicity
surfactant
XPS result
Opis:
In this paper, flotation performances of low-rank coal were investigated in the 2-ethyl hexanol, DAH (dodecyl amine hydrochloride), and SDS (sodium dodecyl sulfate) solutions, respectively. In order to reduce the heterogeneity and hydrodynamic effects on the hydrophobicity and movement trajectory of low-rank coal particles, respectively, experimental coal samples with low ash content and 0.500–0.250 mm in size fraction were adopted. The XPS result demonstrated that the total silicium and aluminum content of 0.500–0.250 mm size fraction was 1.58%. It was also found that the ash content of the 0.500–0.250 mm size fraction was 1.91%. Therefore, it demonstrated that there were few hydrophilic mineral particles on the coal sample surface. Thus, the heterogeneity effect of hydrophilic mineral particles during sliding process measurements can be ignored. The XPS result also indicated that after the grinding process, the mineral content on the low-rank coal surface was very small, which would play a small role in the hydrophobicity of low-rank coal samples. The flotation results indicated that the hydrophobicity of the low-rank coal particles could be improved by nonionic 2-ethyl hexanol and cation DAH surfactants. Moreover, from the analysis of slip angle velocity, it demonstrated that the flotation responses of low-rank coal were depressed by anionic SDS. Furthermore, it was observed that the slip angle velocity can be used to evaluate the effect of surfactant agents on the flotation performance of low-rank coal while the surfactant concentration was more than 10–6 mol/L.
W niniejszym artykule zbadano właściwości flotacyjne węgla niskiej jakości w roztworach 2-etyloheksanolu, DAH (chlorowodorku dodecylu) i SDS (dodecylosiarczanu sodu). W celu zmniejszenia niejednorodności i skutków hydrodynamicznych hydrofobowości i trajektorii ruchu cząstek węgla niskiej jakości przyjęto do doświadczeń próbki węgla o niskiej zawartości popiołu z klasy ziarnowej 0,500–0,250 mm. Wynik XPS wykazał, że całkowita zawartość krzemu i glinu w klasie ziarnowej 0,500–0,250 mm wynosiła 1,58%. Stwierdzono również, że zawartość popiołu w klasie ziarnowej 0,500–0,250 mm wynosiła 1,91%. W związku z tym wykazano, że na powierzchni próbki węgla znajduje się niewiele hydrofilowych cząstek mineralnych. W ten sposób można pominąć efekt heterogeniczności hydrofilowych cząstek mineralnych podczas pomiarów kąta poślizgu. Wynik XPS wskazał również, że po procesie mielenia zawartość minerałów na powierzchni węgla niskiej jakości była bardzo mała, dlatego też występuje słabe oddziaływanie na hydrofobowość tych próbek. Wyniki flotacji wskazują, że hydrofobowość cząstek węgla niskiej jakości można poprawić za pomocą niejonowego 2-etyloheksanolu i kationowych środków powierzchniowo czynnych DAH. Analiza prędkości kąta poślizgu wykazuje, że flotacja węgla niskiej jakości została obniżona przez SDS. Ponadto zaobserwowano, że prędkość kąta poślizgu może być wykorzystana do oceny wpływu środków powierzchniowo czynnych na sprawność flotacji węgla niskiej jakości, gdy ich stężenie jest większe niż 10–6 mol/L.
Źródło:
Gospodarka Surowcami Mineralnymi; 2018, 34, 1; 69-82
0860-0953
Pojawia się w:
Gospodarka Surowcami Mineralnymi
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Boundedness of singular integral operators with holomorphic kernels on star-shaped closed Lipschitz curves
Autorzy:
Gaudry, Garth
Qian, Tao
Wang, Silei
Powiązania:
https://bibliotekanauki.pl/articles/966841.pdf
Data publikacji:
1996
Wydawca:
Polska Akademia Nauk. Instytut Matematyczny PAN
Opis:
The aim of this paper is to study singular integrals T generated by holomorphic kernels defined on a natural neighbourhood of the set ${zζ^{-1}: z, ζ ∈ , z ≠ ζ}$, where is a star-shaped Lipschitz curve, $ ={ exp(iz) : z = x+iA(x), A' ∈ L^{∞}[-π,π], A(-π ) =A(π)}$. Under suitable conditions on F and z, the operators are given by (1) $TF(z)= p.v. ∫_ (zη^{-1})F(η)(dη/η).$ We identify a class of kernels of the stated type that give rise to bounded operators on $L^{2} (,|d|)$. We establish also transference results relating the boundedness of kernels on closed Lipschitz curves to corresponding results on periodic, unbounded curves.
Źródło:
Colloquium Mathematicum; 1996, 70, 1; 133-150
0010-1354
Pojawia się w:
Colloquium Mathematicum
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on the Origins of Textile Materials on the Basis of Analysis of Related Hard Relics from Chinese Excavations
Badanie początków zastosowania materiałów włóknistych na podstawie twardych artefaktów z chińskich wykopalisk
Autorzy:
Tao, Y.
Wang, Q.
Yu, W.
Powiązania:
https://bibliotekanauki.pl/articles/231582.pdf
Data publikacji:
2017
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Biopolimerów i Włókien Chemicznych
Tematy:
textile materials
origins
indirect evidence
hard relics
Chinese excavations
materiały włókniste
artefakt
wykopaliska chińskie
Opis:
Textile materials are widely available in nature, and the original time when humans started to use them must have been very early. However, they also easily aged, and most of the ancient ones had decomposed and disappeared thoroughly before we could find them, resulting that the remaining textile relics cannot reflect entirely the origins of textile materials. Fortunately large numbers of indirect evidence related to ancient textile materials can be found in many unearthed culture relics belonging to the Stone Age. Starting from the basic survival needs of primitive man, in this article, we analysed related hard relics from Chinese excavations made of stone, wood, bone, and so on. And based on this, the time of origin of the textile materials was further speculated upon.
Materiały włókniste są stosowane od bardzo dawna. Jednakże tekstylia szybko się starzeją i większość starożytnych materiałów uległo rozkładowi całkowicie znikając zanim je odnaleziono, co powoduje błędy w datowaniu początku stosowania tekstyliów. Dużą liczbę dowodów pośrednich związanych z dawnymi tekstyliami można znaleźć w wielu odnalezionych reliktach kultury pochodzących z różnych epok, w tym epoki kamiennej. W artykule przeanalizowano artefakty pochodzące z chińskich wykopalisk wykonane z kamienia, drewna, kości, ceramiki itp. Na podstawie tychże analiz można określić, z którego okresu pochodzą tekstylia.
Źródło:
Fibres & Textiles in Eastern Europe; 2017, 1 (121); 108-112
1230-3666
2300-7354
Pojawia się w:
Fibres & Textiles in Eastern Europe
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on Railway Track Edge Detection Based on BM3D and Zernike Moments
Autorzy:
Wang, Nan
Huo, Tao
Zhang, Tianming
Powiązania:
https://bibliotekanauki.pl/articles/32378338.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
rail track
edge detection
BM3D
Zernike moments
Canny's operator
tor kolejowy
krawędzie
moment Zernika
Opis:
With the rapid development of intelligent rail transportation, the realization of intelligent detection of railroad foreign body intrusion has become an important topic of current research. Accurate detection of rail edge location, and then delineate the danger area is the premise and basis for railroad track foreign object intrusion detection. The application of a single edge detection algorithm in the process of rail identification is likely to cause the problem of missing important edges and weak gradient change edges of railroad tracks. It will affect the subsequent detection of track foreign objects. A combined global and local edge detection method is proposed to detect the edges of railroad tracks. In the global pixel-level edge detection, an improved blok-matching and 3D filtering (BM3D) algorithm combined with bilateral filtering is used for denoising to eliminate the interference information in the complex environment. Then the gradient direction is added to the Canny operator, the computational template is increased to achieve non-extreme value suppression, and the Otsu thresholding segmentation algorithm is used for thresholding improvement. It can effectively suppress noise while preserving image details, and improve the accuracy and efficiency of detection at the pixel level. For local subpixel-level edge detection, the improved Zernike moment algorithm is used to extract the edges of the obtained pixel-level images and obtain the corresponding subpixel-level images. It can enhance the extraction of tiny feature edges, effectively reduce the computational effort and obtain the subpixel edges of the orbit images. The experimental results show that compared with other improved algorithms, the method proposed in this paper can effectively extract the track edges of the detected images with higher accuracy, better preserve the track edge features, reduce the appearance of pseudo-edges, and shorten the edge detection time with certain noise immunity, which provides a reliable basis for subsequent track detection and analysis.
Źródło:
Archives of Transport; 2023, 68, 4; 7-20
0866-9546
2300-8830
Pojawia się w:
Archives of Transport
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pinellia ternata agglutinin produced in Bombyx mori cells exhibits bioactivity
Autorzy:
Xu, Tao
Wang, Bo
Wang, Liya
Zhang, Yaozhou
Lv, Zhengbing
Powiązania:
https://bibliotekanauki.pl/articles/1039738.pdf
Data publikacji:
2012
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
Pinellia ternata agglutinin
silkworm bioreactor
plant lectin
Bombyx mori cells
Opis:
Pinellia ternata agglutinin (PTA) is highly homologous to many other monocot mannose-binding lectins which reportedly possess antitumor activities. Its production in silkworm cells has great application potential because the baculovirus expression system can produce post-translationally modified proteins at low cost. In the current study, the pta gene was cloned and expressed in silkworm cells, and the expressed protein was analyzed using a hemagglutination assay. A preliminary in vitro study on its anti-proliferative activity was performed. The results show that the recombinant PTA with an apparent molecular mass of 29 kDa can hemagglutinate rabbit erythrocytes and this activity can be inhibited by D-mannan at a low concentration. In addition, the recombinant hemagglutinin exhibited a dose-dependent anti-proliferative activity on hepatoma cells. The results of the current study suggest that PTA and other important bioactive proteins could be produced by silkworm bioreactor for biomedicine research and application.
Źródło:
Acta Biochimica Polonica; 2012, 59, 2; 231-236
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 45

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