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Tytuł:
Particle Flow Code modeling of the mechanical behavior of layered rock under uniaxial compression
Modelowanie właściwości mechanicznych skał warstwowych w warunkach ściskania jednoosiowego przy zastosowaniu oprogramowania Particle Flow Code(PFC)
Autorzy:
Yao, Nan
Ye, Yi-Cheng
Hu, Bin
Wang, Wei-Qi
Wang, Qi-Hu
Powiązania:
https://bibliotekanauki.pl/articles/218969.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
skała warstwowa
wytrzymałość na ściskanie jednoosiowe
Particle Flow Code (PFC)
kąt uwarstwienia
mikropęknięcia
layered rock
uniaxial compression test
bedding angle
microcracks
Opis:
In this paper, the different mechanical behaviors of layered rocks with different bedding angles during uniaxial compression tests are studied. Numerical simulation models of layered rock are validated based on laboratory tests, and uniaxial compression tests are conducted by using Particle Flow Code (PFC). Using these simulations, the uniaxial compressive strength, failure patterns, development of micro-cracks, and displacement of meso particles are analyzed. When the bedding angle is similar to the failure angle, the macro failure planes develop directly along the beddings, the bedding behavior dictates the behavior of the layered rock, reducing the compressive strength.
W pracy badano właściwości mechaniczne skał warstwowych zalegających pod różnym kątem uwarstwienia w warunkach ściskania jednoosiowego. Walidację modeli symulacyjnych skał warstwowych przeprowadzono w oparciu o wyniki badań laboratoryjnych, zaś testy ściskania jednoosiowego prowadzono z użyciem pakietu Particle Flow Code (PFC). W oparciu o badania symulacyjne, analizowano wytrzy-małość skał na ściskanie jednoosiowe, modele pękania, powstawanie mikropęknięć i przemieszczenia mezo- cząstek. W przypadku gdy kąt płaszczyzny uwarstwienia ma wartość przybliżoną do kąta pęknięcia, płaszczyzny pękania w skali makro pojawiają się wzdłuż spękań, a układ warstw skalnych determinuje ich wytrzymałość na ściskanie, powodując jej obniżenie.
Źródło:
Archives of Mining Sciences; 2019, 64, 1; 181-196
0860-7001
Pojawia się w:
Archives of Mining Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A study of distortion correction algorithms based on aspheric fisheye lens design
Autorzy:
Li, Dan
Zhang, Baolong
Zhu, Jiawei
Wang, Qi
Zhu, Zhenwei
Powiązania:
https://bibliotekanauki.pl/articles/1835810.pdf
Data publikacji:
2020
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
fisheye lens
image processing
distortion correction
imaging optics
Opis:
A design method of aspheric fisheye lens has been proposed in this paper, based on the requirements of automobile surround view system. The study has designed a kind of ultra-wide-angle fisheye lens, which only consists of a spherical glass lens and three aspherical plastic lenses. The maximum diameter of imaging aperture is 15. 3 mm; the working distance behind is 2.158 mm; the total length of system is 11.44 mm; the focal length is 0.97 mm; the viewing angle is 210°, and the modulation transfer function (MTF) curve is 0.35 at 60 lp/mm. Furthermore, a kind of a distortion correction algorithm for fisheye lens has been created, which calculates the position of the ideal image point with the actual image point and the obtained distortion curve and distortion model. The algorithm can correct the distorted image taken by a fisheye lens to an image without distortion, which is suitable for the human eye. The algorithm, which is simple and effective, has been applied to the automobile surround view system. It has been verified to be accurate and reasonable, after the comparison is made between the real image taken by a fisheye lens and the corrected image.
Źródło:
Optica Applicata; 2020, 50, 4; 579-592
0078-5466
1899-7015
Pojawia się w:
Optica Applicata
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polymorphisms in the p53 pathway genes and micronucleus occurrence in Chinese vinyl chloride-exposed workers
Autorzy:
Li, Yong
Feng, Nan-Nan
Zhang, Guang-Hui
Wang, Qi
Hao, Yan-Hui
Nanzhang, Ya
Long, Changxu
Li, Yongliang
Brandt-Rauf, Paul W.
Xia, Zhao-Lin
Powiązania:
https://bibliotekanauki.pl/articles/2179059.pdf
Data publikacji:
2013-12-01
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
VC
occupational exposure
p53 pathway genes
genetic polymorphism
Opis:
Objectives: To investigate the association between polymorphisms in the p53 pathway genes and chromosomal damage in vinyl chloride (VC)-exposed workers. Materials and Methods: Cytokinesis block micronucleus test was performed in 310 VC-exposed workers and 149 non-exposed workers to determine chromosomal damage. The polymerase chain reaction and restriction fragment length polymorphism technique were used to detect six SNPs in the p53 pathway genes involved in the cell cycle. Results: There was a highly significant dose-response relationship between VC exposure and chromosomal damage. Individuals carrying the variant genotypes were at higher risk for chromosomal damage compared with their wild type genotype: p53rs1042522, MDM2 Del1518rs3730485, MDM2rs2279744 and GADD45Ars532446. On the other hand, individuals possessing the variant genotype of CDKN2A rs3088440 had significantly decreased risk compared with the corresponding wild-type. Conclusions: Genetic polymorphisms in P53 pathway genes may have an impact on VC-induced chromosomal damage.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2013, 26, 6; 825-836
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Selective extraction of vanadium from vanadium-titanium magnetite concentrates by non-salt roasting of pellets-H2SO4 leaching process
Autorzy:
Luo, Yi
Che, Xiaokui
Wang, Haixia
Zheng, Qi
Wang, Lei
Powiązania:
https://bibliotekanauki.pl/articles/1445902.pdf
Data publikacji:
2021
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
vanadium-titanium magnetite
pellets
selective extraction
sulfuric acid leaching
Opis:
In this work, a novel process of pellet non-salt roasting and H2SO4 leaching was proposed for the selective extraction of vanadium from vanadium–titanium magnetite concentrate. Vanadium can be leached but the iron impurity was maintained in the pellets. Moreover, the leached pellets can meet the quality requirements of the iron-making process after secondary roasting, realizing comprehensive utilization. The maximal vanadium leaching efficiency was up to 60.3%, whereas 0.17% of the iron impurity was leached. The optimum conditions of pellet roasting and leaching were obtained by single-factor experiments. The X-ray diffraction and scanning electron microscopy–energy disperse X-ray spectrometry analyses showed that the vanadium iron spinel can be oxidized and decomposed into Fe2O3 and vanadate during the roasting process. Given that dilute sulfuric acid can react with vanadate without reacting with Fe2O3 in the leaching process, selective vanadium extraction was realized. This work provides new insights into the industrial production of vanadium–titanium magnetite concentrate involving the direct extraction of vanadium.
Źródło:
Physicochemical Problems of Mineral Processing; 2021, 57, 4; 36-47
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of Asymmetrical Bending Pipe with Different Gating Systems on Low-Pressure Metal Mold Casting
Autorzy:
Wang, Ning
Li, Rong
Zhang, ZiQi
Zeng, Qi
Powiązania:
https://bibliotekanauki.pl/articles/27314123.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
pipe of aluminum
asymmetrical bending
metal mould
low-pressure casting
casting simulation
rura aluminiowa
forma asymetryczna
odlewanie niskociśnieniowe
symulacja odlewania
Opis:
To prepare a high-quality asymmetrical bending pipe of aluminum alloy by casting, the parting surfaces of the asymmetrical parts were determined based on the characteristics of the parts. Also, the forming process was designed and calculated. After that, the different types of gating systems were designed and the casting process was calculated by ProCAST, and then the influence of different casting gating systems on asymmetrical bending pipes was analyzed. The simulation results show that in the solidification process, although the filling speed of the single runner was slow, but the filling was stable. The gating system with a single runner-round flange filling system would lead to being more uniform for filling flow field and be sequential solidification of temperature field distribution, and stronger of the feeding ability. During the solidification process, the solid phase ratio of the single runner-round flange casting system is larger, and the shrinkage volume is smaller, which made the quality of castings better. Finally, a metal mold and core were made to cast a perfect asymmetric bending pipe of aluminum alloy product in a die casting machine. So the single runner-round flange filling system is suitable for asymmetrical bending pipe casting.
Źródło:
Archives of Foundry Engineering; 2023, 23, 1; 83--102
1897-3310
2299-2944
Pojawia się w:
Archives of Foundry Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on chaotic features of a flow field over a plunging airfoil based on dynamic mode decomposition
Autorzy:
Qi, Yuhang
Yang, Wenqing
Wang, Yue
Song, Bifeng
Powiązania:
https://bibliotekanauki.pl/articles/1839716.pdf
Data publikacji:
2021
Wydawca:
Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
Tematy:
plunging airfoil
chaotic flow
dynamic mode decomposition
thrust characteristic
irregularity
Opis:
Periodic motion of a plunging airfoil causes continuous changes in the surrounding flow field. The time-dependent thrust coefficient depends entirely on unsteady characteristics of the flow field. On the contrary, the time-dependent thrust coefficient may also reflect the unsteady characteristics of the corresponding flow field. With the fast Fourier transform (FFT) and dynamic mode decomposition (DMD), unsteady aerodynamic forces can be correlated with the flow field characteristics in the frequency domain. In the present paper, DMD is performed to analyze the unsteady characteristics of the flow field around a plunging NACA0012 airfoil at the Reynolds number of 20 000.
Źródło:
Journal of Theoretical and Applied Mechanics; 2021, 59, 2; 293-305
1429-2955
Pojawia się w:
Journal of Theoretical and Applied Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effects of Neuromedin S on the proliferation of splenic lymphocytes and the cytokine secretion by pulmonary alveolar macrophages in pigs in vitro
Autorzy:
Lin, R.
Wang, Q.
Qi, B.
Huang, Y.
Yang, G.
Powiązania:
https://bibliotekanauki.pl/articles/30295.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Neuromedin S (NMS), a 36-amino acid neuropeptide, has been found to be involved in the regulation of the endocrine activity. It has been also detected in immune tissues in mammals, what suggests that NMS may play an important role in the regulation of immune response. The aim of this study was to demonstrate the presence of NMS receptor 1 (NMU1R) and effect of NMS in pig splenic lymphocytes (SPLs) and pulmonary alveolar macrophages (PAMs). The presence of NMU1R in pig SPLs and PAMs was respectively confirmed by reverse transcription-polymerase chain reaction (RT-PCR), western blot analysis and immunocytochemical methods. Furthermore, SPL proliferation was analyzed using the 3-(4,5)-dimethyl-thiahiazo-(-2-yl)-3,5-di-phenytetrazoliumromide (MTT) method. Additionally, the secretion of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) in PAMs was all measured by enzyme-linked immunosorbent assay (ELISA) kits. In the present study, the results of RT-PCR and western blot analysis revealed that NMU1R mRNA and protein were both expressed in pig SPLs and PAMs, and the immunocytochemical investigations further revealed that the positive signal of NMU1R immunoreactivity was observed in plasma membranes of both SPLs and PAMs. In the in vitro study, we found that at concentrations of 0.001-1000 nM NMS alone or combined with lipopolysaccharide or phytohemagglutinin significantly increased SPL proliferation. Application of ELISA method showed that NMS could induce the secretion of the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in PAMs. These results suggest that NMS can act as a potently positive pro-inflammatory factor and immunomodulatory agent that affects the immune response of immune cells by combining with its receptor NMU1R.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Self-catalysed hydrogenation of heavy oil and coal mixtures
Autorzy:
Li, Suan
Sun, Zhenguang
Liu, Qi
Ye, Hang
Wang, Kunpeng
Powiązania:
https://bibliotekanauki.pl/articles/27315628.pdf
Data publikacji:
2023
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
self-catalysed
heavy oil
hydrogenation
coal liquefaction
co-processing technology
Opis:
Coal liquefaction and heavy oil processing have become the urgent need for national energy strategic technology reserves in China. However, the inactivation of solid catalysts in these processes is an inevitable problem. Therefore,a self-catalysed method was proposed. The properties of raw oil could be changed by adding a modifier, as it has the function of self-catalysis, and the additional catalyst is no longer needed. The effect of 200 ppm modifier onthe hydrogenation of heavy oil and 500 ppm on the hydrogenation of coal and oil were investigated. The results showed that modifiers could be miscible with heavy oil at 50~100 °C and could change the properties of oil. When the temperature exceeded 250 °C, the sulfur element in the heavy oil combined with the metal element broughtin by the modifier to form a particle with the size of 2–8 nm, which could interact with the hydrogen molecule toactivate the hydrogen molecule. Activated hydrogen atoms further formed the complexes with nickel, vanadium,calcium, iron, and other elements in heavy oil to achieve the purpose of purifying and lightening the oil phase.Therefore, the self-catalysed method could be widely used in oil re fining and would greatly promote the development of the oil refining and catalysis industry.
Źródło:
Polish Journal of Chemical Technology; 2023, 25, 2; 8--14
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Research on creep-fatigue model of anchored jointed rock mass
Autorzy:
Song, Yang
Fan, Bo
Li, Yong-Qi
Wang, He-Ping
Powiązania:
https://bibliotekanauki.pl/articles/2200878.pdf
Data publikacji:
2023
Wydawca:
Polskie Towarzystwo Mechaniki Teoretycznej i Stosowanej
Tematy:
shear creep
anchored jointed rock mass
fractional order
fatigue load
damage
Opis:
To explore the mechanical response of anchored rock mass with an interaction of fatigue and creep, this paper selects the fatigue amplitude and fatigue frequency as influencing factors to carry out a fatigue-creep experiment of the anchored rock mass. Based on fractional order theory and binary perturbation theory, a new fractional order creep model is constructed. The results show that compared with the Burgers model, the newly proposed creep model can better describe the whole process of creep deformation of the anchored jointed rock mass under fatigue loading.
Źródło:
Journal of Theoretical and Applied Mechanics; 2023, 61, 1; 163--173
1429-2955
Pojawia się w:
Journal of Theoretical and Applied Mechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Language and Cultural Peculiarities of Gender Realisation
Autorzy:
Baghana, Jerome
Slobodova Novakova, Katarina
Voloshina, Tatiana
Qi, Wang
Pavlikova, Martina
Powiązania:
https://bibliotekanauki.pl/articles/18105015.pdf
Data publikacji:
2022-09-27
Wydawca:
Fundacja Pro Scientia Publica
Tematy:
linguistic and cultural gender realisation
core of gender stereotypes
adverts
social and language environment
magazines
Opis:
Aim. The aim of the article is to reveal gender realisation peculiarities in English and German advertising texts. The concept of the research work is focused on the idea that representatives of different languages and cultures make unique world environment. The work proves the concept validity of the unique language nature in the distinctive idea of its cognitive base formed by an ethnic group. Methods. The concepts of universality and uniqueness of the linguistic and cultural features of gender perception are revealed using the methods of analysis and generalisation. Results. The result of the research work is in the necessity for advertising texts to have special gender language markers which are the kind of signal influencing the conscience of men and women while making purchases. The authors stress that social characteristics of men and women, such as age, social or family status expressed in the form of various degrees of comparison of adjectives are used mainly in English magazines; while German advertising texts put the use of adjectives and articles on the first place. Advertising for women have the set of a special expressive and emotional language tools complex while for men the use of vocabulary stressing the main type of activity of a man - energy is of the top priority. Conclusion. The research work presents cognitive value in theory and practical recommendations to gender linguistics specialising in advertising texts structure illustrating peculiar features of men and women as consumers being influenced by language, cultural, psychological characteristics.
Źródło:
Journal of Education Culture and Society; 2022, 13, 2; 531-548
2081-1640
Pojawia się w:
Journal of Education Culture and Society
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seismic damage behaviour of aeolian sand reinforced concrete columns with I-shaped structural steel
Autorzy:
Wang, Y. H.
Wang, H. Y.
Wang, Y. P.
Zhao, M.
Qi, J.
Huo, G. Z.
Liu, P. Q.
Powiązania:
https://bibliotekanauki.pl/articles/1849772.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
piasek eoliczny
słup żelbetowy
stal konstrukcyjna
przekrój I
zachowanie
uszkodzenie sejsmiczne
model uszkodzenia
aeolian sand
column
I-shaped steel
structural steel
seismic damage
behaviour
damage model
Opis:
To promote the application of aeolian sand resources for steel-concrete composite structures, an aeolian sand reinforced concrete column with I-shaped structural steel is proposed in this study. Four specimens are designed and manufactured with different replacement rates of aeolian sand. The seismic behaviour and damage evolution process of the specimens are studied by low-cycle repeated loading tests. Based on the test results, the mechanical characteristics, failure modes, hysteresis curves, skeleton curves, energy dissipation capacity, displacement ductility, and stiffness degradation of the specimens with different replacement rates of aeolian sand are analysed. In addition, the effects of the design parameters on the seismic behaviour of the specimens are also studied. The results show that the indexes of the seismic behaviour can be significantly improved by adding steel. Moreover, a revised damage model is proposed, to better reflect the evolution law of seismic damage of aeolian sand reinforced concrete columns with steel. The proposed model can provide an important reference for seismic damage assessment of the columns.
Źródło:
Archives of Civil Engineering; 2020, 66, 4; 633-650
1230-2945
Pojawia się w:
Archives of Civil Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of recombinant expression of Bombyx mori bidensovirus ns1 using a modified vector
Autorzy:
Li, Guohui
Li, Mangmang
Wang, Peng
Hu, Zhaoyang
Yao, Qin
Tang, Qi
Chen, Keping
Powiązania:
https://bibliotekanauki.pl/articles/1039216.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
BmBDV
NS1
baculovirus expression vector
Sf9
egfp
Opis:
ns1 gene of Bombyx mori bidensovirus (BmBDV) consisted of 951 nucleotides encoding a deduced 316-amino aicd protein. In this study, the gene was cloned and fused in frame with a N-terminal 6×His tag under control of the polyhedrin promoter, which was transposed into the mini-attTn7 locus of a modified baculovirus vector. Transfection of Sf-9 cells with the resulting recombinant DNA was performed to prepare recombinant virus and the resultant supernatant of transfection with fluorescent signal was harvested. Western blot analysis revealed that NS1 protein was successfully expressed in Sf9 cells infected with the recombinant virus and was confirmed by LC-MS/MS analysis. Moreover, the expressed NS1 is a phosphorylated protein and the phosphorylation site is Thr-184. These results showed that the activity of BmBDV NS1 may be regulated by phosphorylation.
Źródło:
Acta Biochimica Polonica; 2014, 61, 4; 787-794
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The irp2 and fyuA genes in High Pathogenicity Islands are involved in the pathogenesis of infections caused by avian pathogenic Escherichia coli (APEC)
Autorzy:
Tu, J.
Hue, T.
Qi, K.
Shao, Y.
Huang, B.
Wang, X.
Zhou, X.
Powiązania:
https://bibliotekanauki.pl/articles/30327.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Avian pathogenic Escherichia coli (APEC) is a major bacterial infectious disease that may lead to local or systemic infections in chickens with clinical manifestations. The irp2-fyuA gene cluster has been confirmed to be the main genes involved in the synthesis of HPI. The objective of this study was to determine the influence of the irp2 and fyuA genes in the high pathogenicity island (HPI) of avian pathogenic Escherichia coli (APEC) on its pathogenicity by knocking out these genes. The ΔAE17 (lacking irp2) and ΔΔAE17 (lacking irp2 and fyuA) strains of APEC were constructed. The ΔAE17 and ΔΔAE17 strains showed significantly impaired capacity to adhere onto DF-1 cells. The LD50 results indicated that the virulence of the ΔAE17 and ΔΔAE17 strains was decreased in comparison with that of the AE17 strain. We concluded that the knock-out of the core HPI genes weakened APEC adhesion onto DF-1 cells, inhibited transcription of virulence genes, and reduced pathogenicity in chicks. The effects of genetic deletion of irp2 and fyuA on APEC were more severe than those produced by deletion of irp2 only, indicating that irp2 and fyuA co-regulate APEC pathogenicity.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Error analysis of the three-phase electrical energy calculation method in the case of voltage-loss failure
Autorzy:
Cheng, Han-miao
Wang, Zhong-dong
Cai, Qi-xin
Lu, Xiao-quan
Gao, Yu-xiang
Song, Rui-peng
Tian, Zheng-qi
Mu, Xiao-xing
Powiązania:
https://bibliotekanauki.pl/articles/221096.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
three-phase electrical energy meter
voltage-loss failure
voltage substitution
three-phase voltage unbalance
calculation error
Opis:
The single-phase voltage loss is a common fault. Once the voltage-loss failure occurs, the amount of electrical energy will not be measured, but it is to be calculated so as to protect the interest of the power supplier. Two automatic calculation methods, the power substitution and the voltage substitution, are introduced in this paper. Considering the lack of quantitative analysis of the calculation error of the voltage substitution method, the grid traversal method and MATLAB tool are applied to solve the problem. The theoretical analysis indicates that the calculation error is closely related to the voltage unbalance factor and the power factor, and the maximum calculation error is about 6% when the power system operates normally. To verify the theoretical analysis, two three-phase electrical energy metering devices have been developed, and verification tests have been carried out in both the lab and field conditions. The lab testing results are consistent with the theoretical ones, and the field testing results show that the calculation errors are generally below 0.2%, that is correct in most cases.
Źródło:
Metrology and Measurement Systems; 2019, 26, 3; 505-516
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Newly identified transcripts of UL4 and UL5 genes of human cytomegalovirus
Autorzy:
Gao, Shuang
Ruan, Shan
Ma, Yanping
Li, Mali
Wang, Lin
Zheng, Bo
Qi, Ying
Sun, Zhengrong
Huang, Yujing
Ruan, Qiang
Powiązania:
https://bibliotekanauki.pl/articles/1039141.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
HCMV
UL4
UL5
transcript
Opis:
Human cytomegalovirus (HCMV) UL4 and UL5 genes are two members of the RL11 gene family. In an earlier study, three UL4 transcripts of about 1.7, 1.5 and 1.4 kb were found in early and late classes after infection by the Towne strain by nuclease protection and primer extension analyses. In the present study, two UL4 transcripts (1.5 and 1.7 kb) were found by cDNA library screening, Northern blot, 3' and 5' RACE analyses to appear initially in the immediate early phase and one UL4 transcript (1.4 kb) in the late phase in a low-passage clinical isolate. Furthermore, two novel low-abundance UL5 transcripts with the same 3' terminus as the identified UL4 transcripts in the UL4-UL5 gene region were found in late class RNAs.
Źródło:
Acta Biochimica Polonica; 2015, 62, 1; 97-101
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
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