The irp2 and fyuA genes in High Pathogenicity Islands are involved in the pathogenesis of infections caused by avian pathogenic Escherichia coli (APEC)
The irp2 and fyuA genes in High Pathogenicity Islands are involved in the pathogenesis of infections caused by avian pathogenic Escherichia coli (APEC)
Avian pathogenic Escherichia coli (APEC) is a major bacterial infectious disease that may lead to
local or systemic infections in chickens with clinical manifestations. The irp2-fyuA gene cluster has
been confirmed to be the main genes involved in the synthesis of HPI. The objective of this study was
to determine the influence of the irp2 and fyuA genes in the high pathogenicity island (HPI) of avian
pathogenic Escherichia coli (APEC) on its pathogenicity by knocking out these genes. The ΔAE17
(lacking irp2) and ΔΔAE17 (lacking irp2 and fyuA) strains of APEC were constructed. The ΔAE17
and ΔΔAE17 strains showed significantly impaired capacity to adhere onto DF-1 cells. The LD50
results indicated that the virulence of the ΔAE17 and ΔΔAE17 strains was decreased in comparison
with that of the AE17 strain. We concluded that the knock-out of the core HPI genes weakened
APEC adhesion onto DF-1 cells, inhibited transcription of virulence genes, and reduced pathogenicity
in chicks. The effects of genetic deletion of irp2 and fyuA on APEC were more severe than those
produced by deletion of irp2 only, indicating that irp2 and fyuA co-regulate APEC pathogenicity.
Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies
Informacja
SZANOWNI CZYTELNICY!
UPRZEJMIE INFORMUJEMY, ŻE BIBLIOTEKA FUNKCJONUJE W NASTĘPUJĄCYCH GODZINACH:
Wypożyczalnia i Czytelnia Główna: poniedziałek – piątek od 9.00 do 19.00