- Tytuł:
- Graphene oxide flake activation via divinylsulfone – a procedure for efficient β-galactosidase immobilization
- Autorzy:
- Trusek, Anna
- Powiązania:
- https://bibliotekanauki.pl/articles/779087.pdf
- Data publikacji:
- 2019
- Wydawca:
- Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
- Tematy:
-
graphene oxide
enzyme chemical immobilization
divinylsulfone
β-galactosidase
lactose-free product - Opis:
- Flaky graphene oxide was activated with divinylsulfone followed by immobilization of the β-galactosidase enzyme. An active and stable preparation was obtained. β-galactosidase stability after immobilization was much higher than with the native enzyme. The half-life time of the immobilized enzyme was estimated as 165 hours, while for the native form, the estimate was only 5 hours. The developed procedure for the preparation of flaked graphene and its use in the chemical immobilization of enzymes can be used for any enzyme. A processing solution for continuous operation was proposed and verified using cow’s milk, with lactose as the hydrolysed substrate, as a dosing stream. Lactose, a milk sugar, was effectively hydrolysed. Product for allergy sufferers who cannot digest lactose has been obtained in this way.
- Źródło:
-
Polish Journal of Chemical Technology; 2019, 21, 1; 27-32
1509-8117
1899-4741 - Pojawia się w:
- Polish Journal of Chemical Technology
- Dostawca treści:
- Biblioteka Nauki
Artykuł