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Wyświetlanie 1-15 z 27
Tytuł:
Development of an indirect enzyme-linked immunosorbent assay for the detection of novel chicken orthoreovirus
Autorzy:
Wei, Z.
Yang, J.
Hu, J.
Tang, Y.
Diao, Y.
Powiązania:
https://bibliotekanauki.pl/articles/16539093.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
novel avian orthoreovirus
σC gene
recombinant plasmid
indirect enzyme linked immunosorbent assay
epidemiological investigation
Opis:
A novel avian orthoreovirus (N-ARV) variant characterized with obvious arthritis and synovial inflammation, was isolated from Shandong, China in May 2016. It caused chicken poor growth and enormous economic losses to the poultry industry of China. However, there are few effective methods for detecting the antibody levels of N-ARV. In this study, a viral structural protein σC was expressed using the prokaryotic expression vector pET32a (+). The target protein was obtained by inducing for 6 hours at an IPTG concentration of 0.6mM. The optimal dilution of the coating antigen and serum antibody were determined to be 1000 fold and 10 fold respectively. A specificity test showed that there was no positive reactivity between N-ARV and other pathogens, and when the positive serum was diluted 100 times detection results were still checkable. The repeatability of this method was determined by the inter assay and intra assay tests with variability ranging from 4.85% to 7.93%. In conclusion, this indirect enzyme linked immunosorbent assay (ELISA) will be useful for large-scale serological surveys and monitoring antibody levels in N-ARV infection.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 109-118
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Protective effect of lithium chloride on pulmonary injury caused by Actinobacillus pleuropneumoniae via inhibition of GSK-3β-NF-κB-dependent pathway
Autorzy:
Zhang, Y.
Xu, W.
Tang, Y.
Huang, F.
Powiązania:
https://bibliotekanauki.pl/articles/16539075.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
porcine contagious pleuropneumonia
mouse model
lithium chloride
toll-likereceptor 4
GSK-3β-NF-κB-dependent pathways
Opis:
Porcine contagious pleuropneumonia (PCP) is a very serious respiratory disease which is difficult to prevent and treat. In this study, the therapeutic effects of lithium chloride (LiCl) on PCP were examined using a mouse model. A mouse model of PCP was established by intranasal infections with Actinobacillus pleuropneumoniae (App). Histopathological analysis was performed by routine paraffin sections and an H-E staining method. The inflammatory factors, TLR4 and CCL2 were analyzed by qPCR. The expression levels of p-p65 and pGSK-3ß were detected using the Western Blot Method. The death rates, clinical symptoms, lung injuries, and levels of TLR-4, IL-1ß, IL-6, TNF-α, and CCL2 were observed to decrease in the App-infected mice treated with LiCl. It was determined that the LiCl treatments had significantly reduced the mortality of the App-infected cells, as well as the expressions of p-p65 and pGSK-3ß. The results of this study indicated that LiCl could improve the pulmonary injuries of mice caused by App via the inhibition of the GSK-3β-NF-κB-dependent pathways, and may potentially become an effective drug for improving pulmonary injuries caused by PCP.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 35-44
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The expression profile of miR-222b-5p/MAPK10 in spleens of SPF chickens infected with REV-SNV at 28-42 dpi
Autorzy:
Jiang, H.
Gao, S.
Mao, M.
Diao, Y.
Tang, Y.
Hu, J.
Powiązania:
https://bibliotekanauki.pl/articles/2087117.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
reticuloendotheliosis virus strain SNV
RLRs signaling pathway
gga-miR-222b-5p
mitogen-activated protein kinase 10
a dual-luciferase reporter gene experiment
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 3; 439-443
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bacterial communities in PM2.5 and PM10 in broiler houses at different broiler growth stages in spring
Autorzy:
Zhang, J.
Li, Y.
Xu, E.
Jiang, L.
Tang, J.
Li, M.
Zhao, X.
Chen, G.
Zhu, H.
Yu, X.
Zhang, X.
Powiązania:
https://bibliotekanauki.pl/articles/2087465.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bacterial communities
broilers
high-throughput sequencing
particulate matter
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 495-504
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Catalytic oxidation of lignin to aromatics over salen-porphyrin complex as a biomimetic catalyst
Autorzy:
Lu, X.-J.
Zhou, X.-F.
Zhu, Z.-L.
Sun, Y.
Tang, K.
Lei, F.-H.
Liu, Z.-G.
Wang, T.
Powiązania:
https://bibliotekanauki.pl/articles/52771.pdf
Data publikacji:
2019
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Technologii Drewna
Źródło:
Drewno. Prace Naukowe. Doniesienia. Komunikaty; 2019, 62, 203
1644-3985
Pojawia się w:
Drewno. Prace Naukowe. Doniesienia. Komunikaty
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biological characteristics of a new antibacterial peptide and its antibacterial mechanisms against Gram-negative bacteria
Autorzy:
Pei, Z.
Ying, X.
Tang, Y.
Liu, L.
Zhang, H.
Liu, S.
Zhang, D.
Wang, K.
Kong, L.
Gao, Y.
Ma, H.
Powiązania:
https://bibliotekanauki.pl/articles/2087656.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antimicrobial peptide
biological characteristics
antibacterial mechanism
Gram-negative bacteria
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 533-542
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation on Curing Properties and Kinetics of Isophthalonitrile Oxide
Autorzy:
Fan, Y.
Tang, C.
Hu, Q.
Lei, Y.
Huo, J.
Powiązania:
https://bibliotekanauki.pl/articles/780045.pdf
Data publikacji:
2018
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
N,N-dihydroxybenzene-1,3-dicarboximidoyl dichloride
kinetics
elastomer
synthesis
1,3-dipolar cycloaddition
Opis:
N,N-dihydroxybenzene-1,3-dicarboximidoyl dichloride was synthesized from benzene-1,3-dicarboxaldehyde and characterized by fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance  1H and 13C NMR). The elastomer was prepared through the 1,3-dipolar cycloaddition of reaction between liquid polybutadiene (LPB) and isophthalonitrile oxide in this work. The tensile strength of different elastomer was enhanced from 0.14 MPa to 0.33 MPa as the elongation at break decreased from 145% to 73%, and the modulus increased from 0.09 kPa to 0.47 kPa. The parameters of kinetic indicated that the curing reaction was first order reaction and the apparent activation energy of each curing system was less than 10.10 kJ/mol when the content of N,N-dihydroxybenzene-1,3- dicarboximidoyl dichloride was increased from 7% to 12%. These results suggested that nitrile oxides achieved curing of polymer binders at room temperature and this work had definite guiding significance for the application of nitrile oxides in polymer binders.
Źródło:
Polish Journal of Chemical Technology; 2018, 20, 3; 37-46
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Knitted silk mesh-like scaffold incorporated with sponge-like regenerated silk fibroin/collagen I and seeded with mesenchymal stem cells for repairing Achilles tendon in rabbits
Autorzy:
Tang, L.
Yang, Y.
Li, Y.
Yang, G.
Luo, T.
Xue, Y.
Zhang, W.
Powiązania:
https://bibliotekanauki.pl/articles/306523.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
ścięgno Achillesa
kolagen
komórki macierzyste
szpik kostny
Bombyx mori silk
regenerated silk fibroin
collagen I
bone marrow-derived mesenchymal stem cells
weft-knit
Achilles tendon action
Opis:
A scaffold knit with natural sericin-free silk fibroin fiber possesses desirable mechanical properties, biocompatibility, ease of fabrication, and slow degradability. However, regenerated silk fibroin degrades faster than natural silk. In this study, natural silk fibroin fiber mesh-like scaffolds were prepared by a weft-knitting method and the pores were filled with sponge-like regenerated silk fibroin-collagen I. The microporous sponge and mesh-like scaffolds were fused to achieve gradient degradation of the scaffolds, and rabbit bone marrow mesenchymal stem cells (BMSCs) were seeded onto the scaffolds to form scaffold–BMSCs composites. The composites were implanted into gap defects made in the rabbit Achilles tendon. Twenty weeks after implantation, histological observation showed that tendon-like tissue had formed, collagen I mRNA was expressed, abundant collagen was generated, and that there was no obvious degradation of silk. The maximum load of the neo-Achilles tendon was 62.14% that of the natural Achilles tendon. These outcomes were superior to those obtained in the group implanted with a scaffold without BMSCs. These findings suggest the feasibility of constructing tissue-engineered tendons using weft-knitted silk scaffolds incorporated with sponge-like regenerated silk fibroin/collagen I and seeded with BMSCs, and show potential of the scaffold–BMSCs composites to repair Achilles tendon defects.
Źródło:
Acta of Bioengineering and Biomechanics; 2018, 20, 3; 77-87
1509-409X
2450-6303
Pojawia się w:
Acta of Bioengineering and Biomechanics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of polyacrylic acid on the surface properties of calcite and fluorite aiming at their selective flotation
Autorzy:
Zhang, C.
Gao, Z.
Hu, Y.
Sun, W.
Tang, H.
Yin, Z.
He, J.
Guan, Q.
Zhu, Y.
Powiązania:
https://bibliotekanauki.pl/articles/110577.pdf
Data publikacji:
2018
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
calcite
fluorite
selective flotation
polyacrylic acid
Opis:
In this study, the polyacrylic acid (PAA) was studied as a selective depressant for calcite in the selective flotation of fluorite and calcite, and the implications of this process for the separation of fluorite ore were studied using micro-flotation tests, and the results were analyzed with Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) analyses. The flotation tests indicated that the preferential adsorption of PAA onto calcite rather than sodium oleate (NaOl) could selectively depress the flotation of calcite, allowing its separation from fluorite at pH 7. The zeta potential of calcite became more negative with the addition of PAA rather than with NaOl. However, the characteristic features of PAA adsorption were not observed for fluorite, suggesting that NaOl preferentially adsorbed on the surface of fluorite, or that PAA could be replaced by NaOl on the fluorite surface. FT-IR and XPS analysis were utilized to obtain a better understanding of the mechanism by which PAA was more strongly adsorbed on the calcite surface than NaOl. This was revealed to occur through chemical bonding between the carboxyl group of PAA and the hydroxyl groups of the Ca species on the calcite surface, modifying the structure of the adsorbed layer. A possible adsorption mechanism, along with a postulated adsorption mode for the surface interaction between PAA and calcite, is proposed.
Źródło:
Physicochemical Problems of Mineral Processing; 2018, 54, 3; 868-877
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Abnormal prediction of dense crowd videos by a purpose-driven lattice Boltzmann model
Autorzy:
Xue, Y.
Liu, P.
Tao, Y.
Tang, X.
Powiązania:
https://bibliotekanauki.pl/articles/329703.pdf
Data publikacji:
2017
Wydawca:
Uniwersytet Zielonogórski. Oficyna Wydawnicza
Tematy:
video surveillance
crowd analysis
abnormal events
lattice Boltzmann model
purpose driven strategy
monitoring wizyjny
analiza tłumu
zdarzenie nieprawidłowe
Opis:
In the field of intelligent crowd video analysis, the prediction of abnormal events in dense crowds is a well-known and challenging problem. By analysing crowd particle collisions and characteristics of individuals in a crowd to follow the general trend of motion, a purpose-driven lattice Boltzmann model (LBM) is proposed. The collision effect in the proposed method is measured according to the variation in crowd particle numbers in the image nodes; characteristics of the crowd following a general trend are incorporated by adjusting the particle directions. The model predicts dense crowd abnormal events in different intervals through iterations of simultaneous streaming and collision steps. Few initial frames of a video are needed to initialize the proposed model and no training procedure is required. Experimental results show that our purpose-driven LBM performs better than most state-of-the-art methods.
Źródło:
International Journal of Applied Mathematics and Computer Science; 2017, 27, 1; 181-194
1641-876X
2083-8492
Pojawia się w:
International Journal of Applied Mathematics and Computer Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Crystallization and melting behavior of poly(L-lactic acid) modified with salicyloyl hydrazide derivative
Krystalizacja i topnienie poli(kwasu L-mlekowego) modyfikowanego salicyloilową pochodną hydrazydu
Autorzy:
Cai, Y.-H.
Tian, L.
Tang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/945863.pdf
Data publikacji:
2017
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Chemii Przemysłowej
Tematy:
poly(L-lactic acid)
salicyloyl hydrazide derivatives
crystallization
melting
nucleation
poli(kwas L-mlekowy)
salicyloilowe pochodne hydrazydu
krystalizacja
topnienie
enukleacja
Opis:
This work evaluated the influence of a novel organic nucleating agent, salicyloyl hydrazide derivative (BS), on the crystallization and melting behavior of poly(L-lactic acid) (PLLA). Differential scanning calorimetry revealed that the incorporation of BS (0.2 to 3 wt %) significantly promoted the crystallization of PLLA on cooling at 1 °C/min. At BS content of 1.5 wt % in PLLA matrix, the highest effectiveness of crystallization process was observed, this result was further demonstrated by X-ray diffraction analysis of non-isothermal crystallization behavior at different cooling rate and measurements after isothermal crystallization. X-ray diffraction studies showed also that the addition of BS did not change the crystalline structure of PLLA. Based on the effect of the predetermined melting temperature on the crystallization behavior of PLLA/BS, the optimum process temperature of 190 °C was found. The comparative study on the melting behavior of PLLA/BS composite under different conditions (cooling rate, melting temperature) also confirmed the ability of BS to promote the nucleation of PLLA crystallization. The crystallization of PLLA/BS composite depends on the concentration of BS additive, crystallization temperature and time as well as heating rate.
Badano wpływ nowego organicznego czynnika nukleującego, salicyloilowej pochodnej hydrazydu (BS), na krystalizację i topnienie poli(kwasu L-mlekowego) (PLLA). Na podstawie badań metodą różnicowej kalorymetrii skaningowej stwierdzono, że wprowadzenie dodatku BS (0,2–3,0 % mas.) wyraźnie promuje krystalizację PLLA w warunkach chłodzenia z szybkością 1 °C/min. Zawartość 1,5 % mas. BS w osnowie PLLA przyczyniała się do zwiększenia efektywności procesu krystalizacji, co potwierdziły wyniki badań metodą dyfrakcji rentgenowskiej krystalizacji nieizotermicznej w warunkach zmiennej szybkości chłodzenia oraz krystalizacji izotermicznej. Ponadto badania te wykazały, że dodatek BS do PLLA nie zmienia struktury krystalicznej poli(kwasu L-mlekowego). Na podstawie wpływu ustalonych warunków topnienia na przebieg krystalizacji PLLA/BS stwierdzono też, że optymalna temperatura tego procesu wynosi 190 °C. Badania porównawcze topnienia kompozytu PLLA/BS w różnych warunkach (szybkości chłodzenia, temperatury topnienia) również wskazują na zdolność BS do zarodkowania krystalizacji PLLA. Stwierdzono, że krystalizacja kompozytu PLLA/BS zależy od stężenia dodatku BS, temperatury krystalizacji, czasu krystalizacji i szybkości ogrzewania próbki.
Źródło:
Polimery; 2017, 62, 10; 734-742
0032-2725
Pojawia się w:
Polimery
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental study on fracturing coal seams using CaO demolition materials to improve permeability
Autorzy:
Tang, Y.
Yuan, L.
Xue, J
Duan, C.
Powiązania:
https://bibliotekanauki.pl/articles/92132.pdf
Data publikacji:
2017
Wydawca:
Główny Instytut Górnictwa
Tematy:
soundless chemical demolition
coal seam
gas drainage
CaO
pokład węgla
odmetanowanie
Opis:
Currently, methane gas disasters in underground coal mines are a major problem which seriously threatens safe mining. This study employed soundless chemical demolition for fracturing coal seams with low permeability. Additionally, the fracturing theory and failure mechanisms of soundless chemical demolition agents were investigated. Materials such as CaO, naphthalene-based water reducer, sodium gluconate and silicate cement were used to prepare the novel soundless chemical demolition agent, whose optimum proportion was discovered to be 90:3:5:7 by carrying out orthogonal experiments. The innovative demolition agent cracked briquettes and the maximum width of cracks reached 16.33 mm, showing significant potential for improving the permeability of coal seams.
Źródło:
Journal of Sustainable Mining; 2017, 16, 2; 47-54
2300-1364
2300-3960
Pojawia się w:
Journal of Sustainable Mining
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
On the Interaction of Resonance And Bragg Scattering Effects for the Locally Resonant Phononic Crystal with Alternating Elastic and Fluid Matrices
Autorzy:
Yuan, B.
Chen, Y.
Jiang, M.
Tang, S.
He, M.
Tu, M.
Powiązania:
https://bibliotekanauki.pl/articles/177103.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
locally resonant phononic crystals
effective speed of sound
coupling band gaps
underwater acoustic materials
Opis:
Three-dimensional (3D) locally resonant phononic crystals (LRPCs) are studied with the aim of optimising the sub-wavelength band gaps of such composites. By analysing their effective acoustic properties, it has been found that the effective acoustic speed of the composite will drop to zero when local resonance arise, and will increase monotonically when Bragg scattering effects occur. Moreover, if the matrix is a low-shear-speed medium, local resonators can significantly reduce the effective acoustic speed of the composite and, therefore, lower the frequency where Bragg scattering effects occur. Hence, a specific LRPC with alternating elastic and fluid matrices is proposed, whose resonance and Bragg gaps are already close in frequency. The fluid matrix behaves as a wave filter, which prevents the shear waves from propagating in the composite. By using the layer-multiple-scattering theory, the coupling behaviour of local resonance and Bragg scattering band gaps has been investigated. Both gaps are enhanced when they move closer to each other. Finally, a gap-coupled case is obtained that displays a broad sub-wavelength band gap. Such proposal excels at the application of underwater acoustic materials since the arrangement of structure can be handily adjusted for tuning the frequency of coupled gap.
Źródło:
Archives of Acoustics; 2017, 42, 4; 725-733
0137-5075
Pojawia się w:
Archives of Acoustics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pre-concentration of vanadium-bearing mica from stone coal by roasting-flotation
Autorzy:
Tang, J.
Zhang, Y.
Bao, S.
Liu, C.
Powiązania:
https://bibliotekanauki.pl/articles/109373.pdf
Data publikacji:
2017
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
roasting
flotation
vanadium bearing mica
stone coal
Opis:
In China, stone coal is an important source of vanadium. The use of roasting–floatation for the pre-concentration of vanadium bearing mica from vanadium bearing stone coal was investigated based on its mineralogical characteristics. The results showed that the vanadium occurred in mica minerals and the main gangue minerals were coal, calcite, and quartz. The pre-concentration process comprises three key steps: roasting, desliming, and flotation. The coal was completely removed by roasting at 700 °C for an hour. Slime was concentrated and the subsequent flotation pulp was improved by desliming. Calcite was removed by reverse flotation and mica was concentrated by positive floatation. During the process, the grade of V2O5 was increased from 0.71% to 1.14%, and 46.18% of the tailings were rejected. The leaching rate of vanadium was increased from 30.49% of raw ore to 69.15% of the concentrate which was an increase of about 40% at the same leaching process. This technique may promote the efficient utilization of stone coal resources.
Źródło:
Physicochemical Problems of Mineral Processing; 2017, 53, 1; 402-412
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of the start-up of natural bio-film colonization of constructed rapid infiltration system
Autorzy:
Xu, W.
Yang, J.
Wang, J.
Jian, Y.
Tang, M.
Powiązania:
https://bibliotekanauki.pl/articles/259259.pdf
Data publikacji:
2016
Wydawca:
Politechnika Gdańska. Wydział Inżynierii Mechanicznej i Okrętownictwa
Tematy:
Constructed Rapid Infiltration System
start-up of natural bio-film colonization
removal rate
COD
Opis:
Abstract: this paper studied the start-up of natural bio-film colonization under the conditions where wet-to-dry ratio was 1:6, hydraulic load was 0.8m3/m2·d, and water temperature was 100C-150C, it took 25d in total to become mature, after the success of bio-film colonization, the removal rate of COD and NH4+-N can be stabilized at 80%, and the removal rate of NH4+-N can reach 90% above. The microorganism was divided into the following stages during bio-film colonization:1. Reversible adhesion of cell on the surface of the carrier, 2.Irreversible adhesion of cell on the surface of the carrier, 3.Division of cell adhering to the surface of the carrier and formation of microcolony adhering to the surface of the carrier, 4.Growth of microcolony adhering to the surface of the carrier to be mature bio-film with three-dimensional structure. The process of natural bio-film colonization can be judged comprehensively according to removal rate of NH4+-N and COD, effluent pH as well as color of microbial film.
Źródło:
Polish Maritime Research; 2016, S 1; 32-35
1233-2585
Pojawia się w:
Polish Maritime Research
Dostawca treści:
Biblioteka Nauki
Artykuł
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