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    Wyświetlanie 1-9 z 9
    Tytuł:
    Newly identified transcripts of UL4 and UL5 genes of human cytomegalovirus
    Autorzy:
    Gao, Shuang
    Ruan, Shan
    Ma, Yanping
    Li, Mali
    Wang, Lin
    Zheng, Bo
    Qi, Ying
    Sun, Zhengrong
    Huang, Yujing
    Ruan, Qiang
    Powiązania:
    https://bibliotekanauki.pl/articles/1039141.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    HCMV
    UL4
    UL5
    transcript
    Opis:
    Human cytomegalovirus (HCMV) UL4 and UL5 genes are two members of the RL11 gene family. In an earlier study, three UL4 transcripts of about 1.7, 1.5 and 1.4 kb were found in early and late classes after infection by the Towne strain by nuclease protection and primer extension analyses. In the present study, two UL4 transcripts (1.5 and 1.7 kb) were found by cDNA library screening, Northern blot, 3' and 5' RACE analyses to appear initially in the immediate early phase and one UL4 transcript (1.4 kb) in the late phase in a low-passage clinical isolate. Furthermore, two novel low-abundance UL5 transcripts with the same 3' terminus as the identified UL4 transcripts in the UL4-UL5 gene region were found in late class RNAs.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 1; 97-101
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Research on communication emitter identification based on semi-supervised dimensionality reduction in complex electromagnetic environment
    Autorzy:
    Ge, Wei
    Qi, Lin
    Tong, Lin
    Zhu, Jun
    Zhang, Jing
    Zhao, Dongyang
    Li, Ke
    Powiązania:
    https://bibliotekanauki.pl/articles/27311449.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    communication emitter identification
    feature extraction
    dimensionality reduction
    VMD
    ESDA
    variational mode decomposition
    exponential semi-supervised discriminant analysis
    identyfikacja emitera komunikacyjnego
    ekstrakcja cech
    redukcja wymiarowości
    rozkład w trybie wariacyjnym
    analiza dyskryminacyjna wykładnicza półnadzorowana
    Opis:
    The individual identification of communication emitters is a process of identifying different emitters based on the radio frequency fingerprint features extracted from the received signals. Due to the inherent non-linearity of the emitter power amplifier, the fingerprints provide distinguishing features for emitter identification. In this study, approximate entropy is introduced into variational mode decomposition, whose features performed in each mode which is decomposed from the reconstructed signal are extracted while the local minimum removal method is used to filter out the noise mode to improve SNR. We proposed a semi-supervised dimensionality reduction method named exponential semi-supervised discriminant analysis in order to reduce the high-dimensional feature vectors of the signals, and LightGBM is applied to build a classifier for communication emitter identification. The experimental results show that the method performs better than the state-of-the-art individual communication emitter identification technology for the steady signal data set of radio stations with the same plant, batch and model.
    Źródło:
    Bulletin of the Polish Academy of Sciences. Technical Sciences; 2023, 71, 4; art. no. e145766
    0239-7528
    Pojawia się w:
    Bulletin of the Polish Academy of Sciences. Technical Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of Metal Oxides on the Thermal Decomposition Kinetics and Mechanisms of HAN/PVA Based Propellants
    Autorzy:
    Hu, Song-qi
    Liu, Xue-li
    Liu, Lin-Lin
    Kang, Bo
    Zhang, Yan
    Powiązania:
    https://bibliotekanauki.pl/articles/27787991.pdf
    Data publikacji:
    2021
    Wydawca:
    Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
    Tematy:
    HAN/PVA-based propellant
    metal oxides
    catalytic behaviour
    kinetic parameters
    Opis:
    The thermal decomposition processes of HAN/PVA-based propellants have been investigated using a simultaneous thermogravimetric analysis (TGA) – differential scanning calorimetry (DSC), coupled with Fourier-Transform Infrared Spectroscopy (FTIR) and Mass Spectrometry (MS) system. The activation energy (Ea), pre-exponential factor A and reaction mechanism function f(α) of the decomposition processes have been determined by non-isothermal and Malek methods. The results showed that the decomposition process of an HAN/PVA sample occurs mainly in the temperature range 202.2~220.1 °C, with a mass loss, heat release and Ea of about 84.8%, 1474.18 and 88.76 kJ·mol–1, respectively. Of the seven metal oxides studied as catalysts, Al2O3, V2O5 and Fe2O3 have significant catalytic effects on an HAN/PVA-based propellant, in lowering the decomposition temperature, with Ea changing from 88.8 to 83.7, 85.6 and 113.6 kJ·mol–1, respectively. The f(α) of both HAN/PVA and HAN/PVA/Al2O3 samples can be expressed as f(α) = (1 – α)2, whereas f(α) = α or f(α) = α/2 fit well for the HAN/PVA/V2O5 and HAN/PVA/Fe2O3 samples.
    Źródło:
    Central European Journal of Energetic Materials; 2021, 18, 3; 322-338
    1733-7178
    Pojawia się w:
    Central European Journal of Energetic Materials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Polymorphisms in the p53 pathway genes and micronucleus occurrence in Chinese vinyl chloride-exposed workers
    Autorzy:
    Li, Yong
    Feng, Nan-Nan
    Zhang, Guang-Hui
    Wang, Qi
    Hao, Yan-Hui
    Nanzhang, Ya
    Long, Changxu
    Li, Yongliang
    Brandt-Rauf, Paul W.
    Xia, Zhao-Lin
    Powiązania:
    https://bibliotekanauki.pl/articles/2179059.pdf
    Data publikacji:
    2013-12-01
    Wydawca:
    Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
    Tematy:
    VC
    occupational exposure
    p53 pathway genes
    genetic polymorphism
    Opis:
    Objectives: To investigate the association between polymorphisms in the p53 pathway genes and chromosomal damage in vinyl chloride (VC)-exposed workers. Materials and Methods: Cytokinesis block micronucleus test was performed in 310 VC-exposed workers and 149 non-exposed workers to determine chromosomal damage. The polymerase chain reaction and restriction fragment length polymorphism technique were used to detect six SNPs in the p53 pathway genes involved in the cell cycle. Results: There was a highly significant dose-response relationship between VC exposure and chromosomal damage. Individuals carrying the variant genotypes were at higher risk for chromosomal damage compared with their wild type genotype: p53rs1042522, MDM2 Del1518rs3730485, MDM2rs2279744 and GADD45Ars532446. On the other hand, individuals possessing the variant genotype of CDKN2A rs3088440 had significantly decreased risk compared with the corresponding wild-type. Conclusions: Genetic polymorphisms in P53 pathway genes may have an impact on VC-induced chromosomal damage.
    Źródło:
    International Journal of Occupational Medicine and Environmental Health; 2013, 26, 6; 825-836
    1232-1087
    1896-494X
    Pojawia się w:
    International Journal of Occupational Medicine and Environmental Health
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of Neuromedin S on the proliferation of splenic lymphocytes and the cytokine secretion by pulmonary alveolar macrophages in pigs in vitro
    Autorzy:
    Lin, R.
    Wang, Q.
    Qi, B.
    Huang, Y.
    Yang, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/30295.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Neuromedin S (NMS), a 36-amino acid neuropeptide, has been found to be involved in the regulation of the endocrine activity. It has been also detected in immune tissues in mammals, what suggests that NMS may play an important role in the regulation of immune response. The aim of this study was to demonstrate the presence of NMS receptor 1 (NMU1R) and effect of NMS in pig splenic lymphocytes (SPLs) and pulmonary alveolar macrophages (PAMs). The presence of NMU1R in pig SPLs and PAMs was respectively confirmed by reverse transcription-polymerase chain reaction (RT-PCR), western blot analysis and immunocytochemical methods. Furthermore, SPL proliferation was analyzed using the 3-(4,5)-dimethyl-thiahiazo-(-2-yl)-3,5-di-phenytetrazoliumromide (MTT) method. Additionally, the secretion of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α) in PAMs was all measured by enzyme-linked immunosorbent assay (ELISA) kits. In the present study, the results of RT-PCR and western blot analysis revealed that NMU1R mRNA and protein were both expressed in pig SPLs and PAMs, and the immunocytochemical investigations further revealed that the positive signal of NMU1R immunoreactivity was observed in plasma membranes of both SPLs and PAMs. In the in vitro study, we found that at concentrations of 0.001-1000 nM NMS alone or combined with lipopolysaccharide or phytohemagglutinin significantly increased SPL proliferation. Application of ELISA method showed that NMS could induce the secretion of the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α in PAMs. These results suggest that NMS can act as a potently positive pro-inflammatory factor and immunomodulatory agent that affects the immune response of immune cells by combining with its receptor NMU1R.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sp100 interacts with phage ΦC31 integrase to inhibit its recombination activity
    Autorzy:
    Lin, Yun
    Li, Zhi-Hui
    Wang, Jing-Jing
    Xu, Gua-Lan
    Shen, Qi
    Tian, Lin
    Xue, Jin-Lun
    Chen, Jin-Zhong
    Powiązania:
    https://bibliotekanauki.pl/articles/1039951.pdf
    Data publikacji:
    2011
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Sp100
    ΦC31 integrase
    recombination
    Opis:
    Phage ΦC31 integrase is a potential vector for the insertion of therapeutic genes into specific sites in the human genome. To understand the mechanism involved in ΦC31 integrase-mediated recombination, it is important to understand the interaction between the integrase and cellular proteins. Using a yeast two-hybrid system with pLexA-ΦC31 integrase as bait, we screened a pB42AD human fetal brain cDNA library for potential interacting cellular proteins. From the 106 independent clones that were screened, 11 potential interacting clones were isolated, of which one encoded C-terminal fragment of Sp100. The interaction between Sp100 and ΦC31 integrase was further confirmed by yeast mating and co-immunoprecipitation assays. The hybridization between a ΦC31 integrase peptide array and an HEK293 cell extract revealed that residues 81RILN84 in the N-terminus of ΦC31 integrase are responsible for the interaction with Sp100. Knocking down endogenous Sp100 with Sp100-specific siRNA increased ΦC31 integrase-mediated recombination but did not impact reporter gene expression. Therefore, endogenous Sp100 may interact with ΦC31 integrase and inhibit the efficiency of ΦC31 integrase-mediated recombination.
    Źródło:
    Acta Biochimica Polonica; 2011, 58, 1; 67-73
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A dynamic model for the heat transfer behavior of a cooling system
    Autorzy:
    Liu, Wen
    Zhang, Chi
    Hu, Xiao-Xiong
    Cao, Jian-Bo
    Liu, Li-Jiao
    Ye, Xin-Y
    Lin, Li
    Qi, Xun-Ji
    Powiązania:
    https://bibliotekanauki.pl/articles/2050060.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Instytut Badań Systemowych PAN
    Tematy:
    adsorption system
    refrigerating system
    desorption
    simulation
    Opis:
    For purposes of studying the heat transfer behavior of various fluids in a refrigeration system, a dynamic model is established, obtained on the basis of analysis concerning the effects of adsorption velocity, adsorbent bed temperature, condensing temperature, and heat transfer fluids, as well as changes of external conditions. It is demonstrated that adsorption velocity increases sharply in the initial phase of adsorption process and gradually declines after reaching a peak value, whereas condensing temperature increases sharply in the initial phase of desorption process and decreases after reaching a peak value with the desorption quantity decreasing. Furthermore, the increase of heat source temperature and the decrease of cooling water temperature can advance the adsorption process. The present study therefore suggests some ways of improving the performance of such a refrigeration system by increasing heat source temperature, decreasing ambient air temperature, increasing return air temperature and decreasing cooling water temperature.
    Źródło:
    Control and Cybernetics; 2020, 49, 3; 315--332
    0324-8569
    Pojawia się w:
    Control and Cybernetics
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Lipopolysaccharide accelerates fine particulate matter-induced cell apoptosis in human lung bronchial epithelial cells
    Autorzy:
    Ru, Qin
    Xiong, Qi
    Chen, Lin
    Tian, Xiang
    Yue, Kai
    Ma, Baomiao
    Liu, Lu
    Wu, Rihui
    Xu, Congyue
    Pi, Mingshan
    Li, Chaoying
    Powiązania:
    https://bibliotekanauki.pl/articles/2161782.pdf
    Data publikacji:
    2017-11-03
    Wydawca:
    Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
    Tematy:
    Apoptosis
    cytotoxicity
    inflammation
    fine particulate matter
    free radicals
    bronchial epithelial cells
    Opis:
    Objectives The aim of the study has been to investigate the effect of the Standard Reference Material of fine particulate matter (SRM 2786) on cytotoxicity and apoptosis in human lung bronchial epithelial cells (16HBE cells). Whether the lipopolysaccharide (LPS)-induced inflammation could further accelerate cell apoptosis induced by SRM 2786 stimulation has also been determined. Material and Methods 16HBE cells were exposed to various doses of SRM 2786 with or without LPS. The following parameters: cytotoxicity, apoptotic rate, Bax/Bcl-2 expression, nitric oxide (NO) production, and reactive oxygen species (ROS) generation were measured. Results The results have shown that SRM 2786 induces cell damage and apoptosis of 16HBE cells as demonstrated by significant decrease in expression of Bcl-2 and increase in expression of Bax. When compared with the control cells, the apoptotic rate of cells treated by 500 μg/ml of SRM 2786 increased from 2.43±0.21% to 43.96±2.95% (p < 0.01). Further, there was an elevated production of NO and ROS post SRM 2786 treatment. The level of NO in cells treated with 500 μg/ml of SRM 2786 was 18.33±1.02 μmol/l whereas that of control cells was 1.58±0.31 μmol/l (p < 0.01). When compared with the control group, the level of intracellular ROS increased by 24% after treatment with 500 μg/ml of SRM 2786 (p < 0.05). In addition, LPS pre-treatment may accelerate cell apoptosis by increasing generation of NO and ROS followed by SRM 2786 stimulation. When compared to cells treated with 125 μg/ml of SRM 2786 alone, the levels of NO and ROS in cells pretreated with LPS increased by 28% and 11.6%, respectively (p < 0.05), and the apoptotic rate increased from 34.62±4.44% to 54.11±3.34% (p < 0.01). Conclusions These findings have suggested that in vitro exposure to SRM 2786 could induce 16HBE cells apoptosis probably by means of the mechanism involving the generation of free radicals, while the degree of apoptosis would be further aggravated under inflammation condition. Int J Occup Med Environ Health 2018;31(2):173–183
    Źródło:
    International Journal of Occupational Medicine and Environmental Health; 2018, 31, 2; 173-183
    1232-1087
    1896-494X
    Pojawia się w:
    International Journal of Occupational Medicine and Environmental Health
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Analysis of the factors affecting the realization of lambda transition temperature of 4He
    Autorzy:
    Yin, L.
    Lin, P.
    Zhao, J. J.
    Qi, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/221031.pdf
    Data publikacji:
    2011
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    4He
    lambda transition temperature
    sealed-cell
    realization
    Opis:
    Owing to the dramatic change in the thermal conductivity of 4He when its temperature crosses the transition of superfluid (HeI) and normalfluid (HeII), a sealed-cell with a capillary is used to realize the lambda transition temperature, Tλ. A small heat flow is controlled through the capillary of the sealed-cell so as to realize the coexistence of HeI and HeII and maintain the stay of HeI/HeII interface in the capillary. A stable and flat lambda transition temperature "plateau" is obtained. Because there is a depression effect of Tλ caused by the heat flow through the capillary, a series of heat flows and several temperature plateaus are made and an extrapolation is applied to determine Tλ with zero heat flow. A rhodium-iron resistance thermometer with series number A34 (RIRT A34) has been used in 24 Tλ -realization experiments to derive Tλ with a standard deviation of 0.022mK, which proves the stability and reproducibility of Tλ.
    Źródło:
    Metrology and Measurement Systems; 2011, 18, 1; 13-23
    0860-8229
    Pojawia się w:
    Metrology and Measurement Systems
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-9 z 9

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